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Paswan, Sunil K., and Anant K. Singh. "Analysis of finishing performance in rotating magnetorheological honing process with the effect of particles motion." Proceedings of the Institution of Mechanical Engineers, Part E: Journal of Process Mechanical Engineering 235, no. 4 (January 26, 2021): 1104–18. http://dx.doi.org/10.1177/0954408921990132.
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The particles used in magnetorheological polishing (MRP) fluid are the key components of the magnetorheological (MR) finishing processes. The rotational magnetorheological honing (R-MRH) process is recently developed as a highly productive MR finishing process which is used for finishing the internal surface of the industrial cylindric components. The involvement of micron-sized abrasive particles of MRP fluid in the finishing operation results in the invisible observation of the finishing mechanism which enables the urge of analyzing the motion of the particles during the present R-MRH process. Therefore, the effect of motions of the MRP-fluid’s particles is analyzed for nano-finishing performance on the inside surface of the cylindric workpieces. The motions performed by active abrasive particles on the inside surface of the rotating hollow cylindric workpiece cause a higher finishing rate. The effects of particle motions on the reduction in surface roughness and improvement in surface morphology confirm the usefulness of the R-MRH process. The surface finish with the effect of the particles' motions of the MRP-fluid in the R-MRH process on the stationary workpiece’s inner surface is achieved upto 100 nm from 420 nm of the initial ground surface in 60 min of finishing. Whereas, the same aforementioned surface of the rotating workpiece is finished upto 50 nm from the same initial ground surface in only 40 min of finishing with the effect of the particles' motions of the MRP-fluid. The improvement in the surface finish is also noticed through the scanning electron micrographs in this work. The significant change in surface finish obtained in experimentations confirms the integrity of the analytical study conducted for understanding the effects of motions of particles while finishing with the R-MRH process.
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Smits, Katrien, Yannick Gansemans, Laurentijn Tilleman, Filip Van Nieuwerburgh, Margot Van De Velde, Ilse Gerits, Cyrillus Ververs, et al. "Maternal Recognition of Pregnancy in the Horse: Are MicroRNAs the Secret Messengers?" International Journal of Molecular Sciences 21, no. 2 (January 9, 2020): 419. http://dx.doi.org/10.3390/ijms21020419.
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The signal for maternal recognition of pregnancy (MRP) has still not been identified in the horse. High-throughput molecular biology at the embryo–maternal interface has substantially contributed to the knowledge on pathways affected during MRP, but an integrated study in which proteomics, transcriptomics and miRNA expression can be linked directly is currently lacking. The aim of this study was to provide such analysis. Endometrial biopsies, uterine fluid, embryonic tissues, and yolk sac fluid were collected 13 days after ovulation during pregnant and control cycles from the same mares. Micro-RNA-Sequencing was performed on all collected samples, mRNA-Sequencing on the same tissue samples and mass spectrometry was conducted previously on the same fluid samples. Differential expression of miRNA, mRNA and proteins showed high conformity with literature and confirmed involvement in pregnancy establishment, embryo quality, steroid synthesis and prostaglandin regulation, but the link between differential miRNAs and their targets was limited and did not indicate the identity of an unequivocal signal for MRP in the horse. Differential expression at the embryo–maternal interface was prominent, highlighting a potential role of miRNAs in embryo–maternal communication during early pregnancy in the horse. These data provide a strong basis for future targeted studies.
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Jayant and V. K. Jain. "Analysis of finishing forces and surface finish during magnetorheological abrasive flow finishing of asymmetric workpieces." Journal of Micromanufacturing 2, no. 2 (April 9, 2019): 133–51. http://dx.doi.org/10.1177/2516598418818260.
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Magnetorheological abrasive flow finishing (MRAFF) is an advanced hybrid process for producing ultrafine finished surfaces. Such surfaces reduce frictional forces and thereby minimize wear and tear to increase functional lifetime of the components. In the present research work, a model has been developed for simulating the results of MRAFF process. First, magnetic field is simulated and then a detailed study on the rheology of the magnetorheological polishing (MRP) fluid is conducted to develop a viscosity model for the flow of non-Newtonian shear thinning fluid. To calculate the forces acting in the process of material removal, the flow of MRP fluid around an asymmetric workpiece (knee joint) in a spatially varying magnetic field is simulated. Finishing forces exerted by the abrasive particles on the workpiece surface are analysed to develop a model for predicting surface roughness. A methodology has been proposed to evolve a variable correction factor to determine active abrasive particles at different locations on the workpiece surface for accurate simulation of surface finish operation. It is found that the magnetic field greatly influences the process performance by governing the viscosity of the MRP fluid and the distribution of the abrasive particles in the medium. During finishing of an asymmetric workpiece, the surface finish obtained at different locations on the workpiece surface is different. The developed model is capable to predict final surface finish within the acceptable accuracy when compared with the experimental results.
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KORDONSKI, W. I., and S. D. JACOBS. "MAGNETORHEOLOGICAL FINISHING." International Journal of Modern Physics B 10, no. 23n24 (October 30, 1996): 2837–48. http://dx.doi.org/10.1142/s0217979296001288.
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The technology of finishing for optics, ceramics, and semiconductors is one of the most promising uses of the magnetorheological effect. It perfectly coupled with computer control, allowing in quantity production the unique accuracy and quality of a polished surface to be achieved. The polishing process may appear as follows. A part rotating on the spindle is brought into contact with an magnetorheological polishing (MRP) fluid which is set in motion by the moving wall. In the region where the part and the MRP fluid are brought into contact, the applied magnetic field creates the conditions necessary for the material removal from the part surface. The material removal takes place in a certain region contacting the surface of the part which can be called the polishing spot or zone. The polishing process comes to the program-simulated movement of the polishing spot over the part surface. The mechanism of the material removal in the contact zone is considered as a process governed by the particularities of the Bingham flow in the contact zone. The problem like the hydrodynamic theory of lubrication is treated for plastic film. As this takes place the shear stresses distribution in the film is obtained from the experimental measurements of the pressure distribution in the contact spot. Reasonable correlation between calculated and experimental magnitudes of the material removal rate for glass polishing lends support to the validity of the approach.
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Michot, Jean-Michel, Marie F. Heremans, Nancy E. Caceres, Marie-Paule Mingeot-Leclercq, Paul M. Tulkens, and Françoise Van Bambeke. "Cellular Accumulation and Activity of Quinolones in Ciprofloxacin-Resistant J774 Macrophages." Antimicrobial Agents and Chemotherapy 50, no. 5 (May 2006): 1689–95. http://dx.doi.org/10.1128/aac.50.5.1689-1695.2006.
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ABSTRACT Ciprofloxacin is the substrate for a multidrug resistance-related protein (MRP)-like multidrug transporter in J774 mouse macrophages, which also modestly affects levofloxacin but only marginally affects garenoxacin and moxifloxacin (J.-M. Michot et al., Antimicrob. Agents Chemother. 49:2429-2437, 2005). Two clones of ciprofloxacin-resistant cells were obtained by a stepwise increase in drug concentration (from 34 to 51 to 68 mg/liter) in the culture fluid. Compared to wild-type cells, ciprofloxacin-resistant cells showed (i) a markedly reduced ciprofloxacin accumulation (12% of control) and (ii) a two- to threefold lower sensitivity to the enhancing effect exerted by MRP-inhibitors (probenecid and MK571) on ciprofloxacin accumulation or by ciprofloxacin itself. ATP-depletion brought ciprofloxacin accumulation to similarly high levels in both wild-type and ciprofloxacin-resistant cells. Garenoxacin and moxifloxacin accumulation remained unaffected, and levofloxacin showed an intermediate behavior. DNA and protein synthesis were not impaired in ciprofloxacin-resistant cells for ciprofloxacin concentrations up to 100 mg/liter (∼85 and 55% inhibition, respectively, in wild-type cells). In Listeria monocytogenes-infected ciprofloxacin-resistant cells, 12-fold higher extracellular concentrations of ciprofloxacin were needed to show a bacteriostatic effect in comparison with wild-type cells. The data suggest that the resistance mechanism is mediated by an overexpression and/or increased activity of the MRP-like ciprofloxacin transporter expressed at a basal level in wild-type J774 macrophages, which modulates both the intracellular pharmacokinetics and activity of ciprofloxacin.
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Zheng, Cheng, Bingsan Chen, Xiaoyu Yan, Yongchao Xu, and Shangchao Hung. "The three-point eccentric magnetorheological polishing technology for hard brittle alumina ceramics." Mechanical Sciences 13, no. 1 (May 20, 2022): 473–83. http://dx.doi.org/10.5194/ms-13-473-2022.
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Abstract. This work presents the design of a pressurised three-point eccentric magnetorheological polishing (MRP) device, for alumina ceramics' hard and brittle characteristics, and a carrier disc magnetic field generator and a single closed-loop uniform magnetic field generator for a more uniform and increased magnetic field distribution. When compared with the traditional gap type, this device considerably enhances polishing efficiency. This apparatus has also been used to explore the mechanism of MRP. Static magnetic field simulations were conducted, and the fundamentals of the three-point eccentric magnetorheological process were addressed. Alumina ceramics were polished with a three-point eccentric wheel MRP equipment. Polishing tests were conducted to explore the effects of rotational speed, working pressure, abrasive type, abrasive particle size and polishing duration on polishing properties, and optimised polishing parameters were established. The surface roughness (Ra) of the samples was dramatically reduced from 500 to 22.41 nm using the three-point eccentric MRP device. The pit markings on the alumina ceramics' surface vanished after polishing. Therefore, the approach has considerable polishing potential for hard and brittle materials that can be nanofabricated with minimal surface sub-damage.
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Lundy, Fionnuala T., Rod Chalk, Philip-John Lamey, Christopher Shaw, and Gerard J. Linden. "Quantitative analysis of MRP-8 in gingival crevicular fluid in periodontal health and disease using microbore HPLC." Journal of Clinical Periodontology 28, no. 12 (July 7, 2008): 1172–77. http://dx.doi.org/10.1111/j.1600-051x.2001.281213.x.
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Lundy, Fionnuala T., Rod Chalk, Philip-John Lamey, Christopher Shaw, and Gerard J. Linden. "Quantitative analysis of MRP-8 in gingival crevicular fluid in periodontal health and disease using microbore HPLC." Journal of Clinical Periodontology 28, no. 12 (December 2001): 1172–77. http://dx.doi.org/10.1034/j.1600-051x.2001.281213.x.
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Achouiti, Ahmed, Thomas Vogl, Anne J. Van der Meer, Ingrid Stroo, Sandrine Florquin, Onno J. de Boer, Johannes Roth, et al. "Myeloid-related protein-14 deficiency promotes inflammation in staphylococcal pneumonia." European Respiratory Journal 46, no. 2 (March 18, 2015): 464–73. http://dx.doi.org/10.1183/09031936.00183814.
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Staphylococcus aureus has evolved as an important cause of pneumonia in both hospital and community settings. Staphylococcal lung infection can lead to overwhelming pulmonary inflammation. During infection, neutrophils release complexes of myeloid-related protein (MRP)8 and MRP14 (MRP8/14). MRP8/14 has been shown to exert pro-inflammatory and chemotactic activity, and to assist in the killing of S. aureus. In the current study we sought to determine the role of MRP8/14 in the host response during S. aureus pneumonia.Pneumonia was induced in wildtype and MRP14-deficient mice (mice unable to form MRP8/14) by intranasal inoculation of 1×107 CFU of S. aureus USA300. Mice were sacrificed at 6, 24, 48 or 72 h after infection for analyses.S. aureus pneumonia was associated with a strong rise in MRP8/14 in bronchoalveolar lavage fluid and lung tissue. Surprisingly, MRP14 deficiency had a limited effect on bacterial clearance and was associated with increased cytokine levels in bronchoalveolar lavage fluid and aggravated lung histopathology. MRP14 deficiency in addition was associated with a diminished transmigration of neutrophils into bronchoalveolar lavage fluid at late time-points after infection together with reduced release of nucleosomes.MRP8/14 serves in an unexpected protective role for the lung in staphylococcal pneumonia.
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Kukreja, Rishabh, Pratyush Tanwar, Vatsal Verma, and Niranjan M S. "Design and Analysis of a Polishing Tool for Different MRP Fluid in the Magnetic Field Assisted Finishing (MFAF) Process." International Journal of Engineering Trends and Technology 68, no. 5 (May 25, 2020): 54–61. http://dx.doi.org/10.14445/22315381/ijett-v68i5p210s.
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Hitomi, J., K. Yamaguchi, Y. Kikuchi, T. Kimura, K. Maruyama, and K. Nagasaki. "A novel calcium-binding protein in amniotic fluid, CAAF1: its molecular cloning and tissue distribution." Journal of Cell Science 109, no. 4 (April 1, 1996): 805–15. http://dx.doi.org/10.1242/jcs.109.4.805.
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We found by using a 45Ca2+ overlay technique a large amount of Ca(2+)-binding activity in bovine amniotic fluid from which a novel calcium-binding protein (CaBP) was purified and is referred to as CAAF1 (calcium-binding protein in amniotic fluid-1), with an apparent molecular mass of 8 kDa determined by N-tris(hydroxymethyl)-methylglycine/ SDS-PAGE. It was structurally homologous with MRP/calgranulin proteins (MRP8/calgranulin A and MRP14/calgranulin B), members of the S100 protein family, which are abundantly found in the cytoplasm of granulocytes and macrophages. CAAF1 lacked the predicted signal peptide sequence, which is consistent with other CaBPs. The tissue and cellular distribution of CAAF1 was determined by monoclonal antibodies developed against this protein. Its immunoreactivity was found in squamous epithelial cells, neutrophils, and some macrophages throughout the fetal body. An especially characteristic staining pattern was obtained in the squamous epithelium, including that of the esophagus, skin and amnion: CAAF1 was detected in the suprabasal squamous epithelial cells undergoing differentiation, but not in the cells in the proliferating basal layer. Northern blot analysis also showed that CAAF1 mRNA was highly expressed in bovine fetal esophagus and skin. On the other hand, our ELISA studies showed that CAAF1 protein was present in amniotic fluid at a concentration of about 120 nM, which was over 30 times as high as that in the fetal serum. These results suggested that CAAF1 is one of the stage-specific proteins in the differentiation of squamous epithelial cells, and that CAAF1 is preferentially produced by fetal squamous epithelial cells, including epidermal keratinocytes and amniotic epithelial cells, and it is stored in the amniotic fluid during embryogenesis.
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Chen, Zhijun, Jisheng Pan, Qiusheng Yan, Zhanliang Huang, Fenglin Zhang, and Shumei Chen. "Study on the rheological and polishing properties of electromagnetic two-phase composite particles with abrasive characteristics." Smart Materials and Structures 31, no. 4 (March 3, 2022): 045012. http://dx.doi.org/10.1088/1361-665x/ac5478.
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Abstract Electromagnetic composite rheological polishing (EMRP) is a new ultra-precision machining technology that combines electro-rheological polishing (ERP) and magneto-rheological polishing (MRP). The key technology of the polishing method is the preparation of electromagnetic composite rheological fluid (EMRF) with both ERP and MRP, especially the preparation of composite particles with electro- and magneto-rheological effects. In this study, the EMRF was prepared by using electromagnetic two-phase particles with abrasive characteristics. The electromagnetic two-phase composite particles are synthesized in two steps: the coupling method and sol-gel method. The two-step method successfully prepared the electromagnetic two-phase composite particles with nano-diamond particles embedded randomly on the surface. The electro-rheological shear stress of EMRF can reach 160.7 Pa when the test parameter voltage is 2.5 kV, and magneto-rheological shear stress of EMRF can reach 4076 Pa when the electromagnet excitation current is 3 A. When a fused silica glass is polished under a single magnetic field, the material removal depth reaches a maximum of 2.7 μm at a radius of 13.5 mm. Under the action of the electromagnetic compound field, the removal profile of the work-piece is smoother, and the material removal depth reaches the maximum value of 2.1 μm at a radius of 10.5 mm. This proves that the stiffness distribution of the polishing pad under the electromagnetic composite field is more dispersed than that under a single field. Therefore, the distribution of electromagnetic two-phase composite particles can be controlled by applying an electromagnetic composite field, which provides a good foundation for the abrasive control technology of EMRP.
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French, Jacqueline A. "The Role of Drug-Resistance Proteins in Medically Refractory Epilepsy." Epilepsy Currents 2, no. 5 (September 2002): 166. http://dx.doi.org/10.1111/j.1535-7597.2002.00062.x.
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Drug Resistance in Epilepsy: Expression of Drug-resistance Proteins in Common Causes of Refractory Epilepsy Sisodiya SM, Lin WR, Harding BN, Squier MV, Thom M. Brain 2002;125(Pt 1):22–31 Epilepsy is resistant to drug treatment in about one third of cases, but the mechanisms underlying this drug resistance are not understood. In cancer, drug resistance has been studied extensively. Among the various resistance mechanisms, overexpression of drug-resistance proteins, such as multidrug resistance gene-1 P-glycoprotein (MDR1) and multidrug resistance–associated protein 1 (MRP1), has been shown to correlate with cellular resistance to anticancer drugs. Previous studies in human epilepsy have shown that MDR1 and MRP1 also may be overexpressed in brain tissue from patients with refractory epilepsy; expression has been shown in glia and neurons, which do not normally express these proteins. We examined expression of MDR1 and MRP1 in refractory epilepsy from three common causes, dysembryoplastic neuroepithelial tumors (DNTs; eight cases), focal cortical dysplasia (FCD; 14 cases), and hippocampal sclerosis (HS; eight cases). Expression was studied immunohistochemically in lesional tissue from therapeutic resections and compared with expression in histologically normal adjacent tissue. With the most sensitive antibodies, in all eight DNT cases, reactive astrocytes within tumor nodules expressed MDR1 and MRP1. In five of eight HS cases, reactive astrocytes within the gliotic hippocampus expressed MDR1 and MRP1. Of 14 cases of FCD, MDR1 and MRP1 expression was noted in reactive astrocytes in all cases. In five FCD cases, MRP1 expression also was noted in dysplastic neurons. In FCD and DNTs, accentuation of reactivity was noted around lesional vessels. Immunoreactivity was always more frequent and intense in lesional reactive astrocytes than in glial fibrillary acidic protein–positive reactive astrocytes in adjacent histologically normal tissue. MDR1 is able to transport some antiepileptic drugs (AEDs), and MRP1 also may do so. The overexpression of these drug-resistance proteins in tissue from patients with refractory epilepsy suggests one possible mechanism for drug resistance in patients with these pathologies. We propose that overexpressed resistance proteins reduce the interstitial concentration of AEDs in the vicinity of the epileptogenic pathology and thereby render the epilepsy caused by these pathologies resistant to treatment with AEDs. P-Glycoprotein and Multidrug Resistance–associated Protein Are Involved in the Regulation of Extracellular Levels of the Major Antiepileptic Drug Carbamazepine in the Brain Potschka H, Fedrowitz M, Loscher W. Neuroreport 2001;12:3557–3560 Despite considerable advances in the pharmacotherapy of epilepsy, about 30% of epilepsy patients are refractory to antiepileptic drugs (AEDs). In most cases, a patient who is resistant to one major AED also is refractory to other AEDs, although these drugs act by different mechanisms. The mechanisms that lead to drug resistance in epilepsy are not known. Recently, overexpression of multidrug transporters, such as P-glycoprotein (PGP) and multidrug resistance–associated protein (MRP), has been reported in surgically resected epileptogenic human brain tissue and suggested to contribute to the drug resistance of epilepsy. However, it is not known to what extent multidrug transporters such as PGP or MRP are involved in transport of AEDs. In the present study, we used in vivo microdialysis in rats to study whether the concentration of carbamazepine in the extracellular fluid of the cerebral cortex can be enhanced by inhibition of PGP or MRP, using the PGP inhibitor verapamil and the MRP inhibitor probenecid. Local perfusion with verapamil or probenecid via the microdialysis probe increased the extracellular concentration of carbamazepine. The data indicate that both PGP and MRP participate in the regulation of extracellular brain concentrations of the major AED carbamazepine.
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Ye, Dongping, Weiguo Liang, Libing Dai, and Yicun Yao. "Moderate Fluid Shear Stress Could Regulate the Cytoskeleton of Nucleus Pulposus and Surrounding Inflammatory Mediators by Activating the FAK-MEK5-ERK5-cFos-AP1 Signaling Pathway." Disease Markers 2018 (June 12, 2018): 1–9. http://dx.doi.org/10.1155/2018/9405738.
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We first applied moderate fluid shear stress to nucleus pulposus cells. The correlation of AP-1 with type II collagen, proteoglycan, Cytokeratin 8 protein, MAP-1, MAP-2, and MAP-4 and the correlation of AP-1 with IL-1β, TNF-α, IL-6, IL-8, MIP-1, MCP-1, and NO were detected. Our results document that moderate fluid shear stress could activate the FAK-MEK5-ERK5-cFos-AP1 signaling pathway. AP1 could downregulate the construct factors of cytoskeleton such as type II collagen, proteoglycan, Cytokeratin 8 protein, MAP-1, MAP-2, and MAP-4 in nucleus pulposus cell after the fluid shear stress was loaded. AP1 could upregulate the inflammatory factors such as IL-1β, TNF-α, IL-6, IL-8, MIP-1, MCP-1, and NO in nucleus pulposus cell after the fluid shear stress was loaded. Taken together, our data suggested that moderate fluid shear stress may play an important role in the cytoskeleton of nucleus pulposus and surrounding inflammatory mediators by activating the FAK-MEK5-ERK5-cFos-AP1 signaling pathway, thereby affecting cell degeneration.
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Tran, Thi Le Quyen, Hussein Anani, Ho Tinh Trinh, Thi Phuong Thao Pham, Van Khoa Dang, Viet My Ho, Nguyen Hai Linh Bui, et al. "Chitinophaga vietnamensis sp. nov., a multi-drug resistant bacterium infecting humans." International Journal of Systematic and Evolutionary Microbiology 70, no. 3 (March 1, 2020): 1758–68. http://dx.doi.org/10.1099/ijsem.0.003968.
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We describe a new multidrug resistant Chitinophaga species that was isolated from patients with type 2 diabetes in Vietnam. Strain BD 01T was cultivated in 2017 from a blood sample of a patient suffering from bacteremia. Strain VP 7442 was isolated in 2018 from a pleural fluid sample of a patient who had presented with lung abscess and pleural effusion. Both strains are aerobic, Gram-negative, non-motile and non-spore-forming. The 16S rRNA gene sequences of both strains are 100 % similar and share a highest 16S sequence identity with Chitinophaga polysaccharea MRP-15T of 97.42 %. Their predominant fatty acid is iso-C15 : 0 (73.8 % for strain BD 01T and 79.8 % for strain VP 7442). The draft genome sizes of strains BD 01T and VP 7442 are 6 308 408 and 6 308 579 bp, respectively. They are resistant to beta-lactams, aminoglycosides, fluoroquinolones, metronidazole, fosfomycin, vancomycin and macrolides, and exhibit 20 and 18 antimicrobial resistance-related genes, respectively. Using the multiphasic taxonogenomic approach, we propose that strains BD 01T (=CSUR P9622=VTCC 70981) and VP 7442 (=CSUR P9623=VTCC 70982) represent a new species, for which we propose the name Chitinophaga vietnamensis sp. nov. Strain BD 01T was chosen as type strain of C. vietnamensis sp. nov.
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Sharma, Vasundhara, Pallavi Chitrans, Mazher Maqusood, Umar Farooq, Sudhir Singh, Shweta R. Sharma, and Imran Ahmad. "Bacteriological profile and their antibiogram from Endotracheal aspirate and Bronchoalveolar lavage fluid samples from intensive care unit patients." IP International Journal of Medical Microbiology and Tropical Diseases 7, no. 4 (November 15, 2021): 257–61. http://dx.doi.org/10.18231/j.ijmmtd.2021.053.
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Bacterial respiratory infections are most commonly causes of illness for all age group patients in ICU. Most of the patients suffer from urosepsis, postoperative disease and lower respiratory infection when admitted in ICU’s. The main purpose of this study is control in ICU’s infection & determine their antibiotic sensitivity pattern for better treatment of patients. Bacteriological profile and their antibiogram from endotracheal aspirate and bronchoalveolar lavage fluid samples from intensive care unit patients.We performed manual culture of Endotracheal aspirate & BAL fluid specimens collected from ICU’s patients. Samples were cultured on Blood agar and Mac-Conkey agar then incubated at 37C for 24 hours. Any growth were further confirmed by Gram stain and appropriate biochemical tests, and then Antibiotic sensitivity test was done. Among the 123 mechanically ventilated patients and 68 of them developed positive samples. In this study 55.3% (68) pathogenic isolates, 4.1% (5) were non pathogenic isolates and 40.6 (50) were no growth isolates in BAL and endotracheal aspirate samples. In which 67.6% (46) male and 32.4% (22) female patients. Older population falling in the 40-50 years of age group contributes the majority (27.64%) of the infection undergoing treatment for LRT infection in ICU’s patients in TMMC & RC, Moradabad, and in 70-80 years of age group (4.06%) was less contributes of this infection. Among the causative agents, were most common among gram negatives and was common among the gram positive isolates. In our study more resistance gram positive antibiotics were CIP, E, LE, and most sensitive antibiotics were VA, LZ, GEN and TEI. And gram negative bacteria most resistant antibiotics were OF, CPM, CAZ, CIP, CTR, MRP, LE, IPM, PIT and most sensitive antibiotics were AK, GEN, CL, TGC. In this study we conclude that Gram-negative bacilli were predominant in BAL fluid and endotracheal aspirates of ICU patients. Out of 68 multidrug resistance isolates, 1.47% were MRSA, 41.17% were ESBL and 57.36% were MBL isolates higher rate of ESBL production seen in spp., and higher rate of MBL production in spp.High incidence of VAP and the potential MDR pathogens are a real threat in our ICU’s. Combined approach of judicious antibiotic usage and training programs to health care personnel might be of help in combatting high incidence of antibiotic resistance in our institute.
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Wu, Yapeng, Min Yang, Linfeng He, Qiang Lin, Meimei Wu, Zhengyao Li, Yuqing Li, and Xiaoguang Liu. "Sparse-View Neutron CT Reconstruction Using a Modified Weighted Total Difference Minimization Method." Applied Sciences 11, no. 22 (November 19, 2021): 10942. http://dx.doi.org/10.3390/app112210942.
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Indirect neutron imaging is an effective method for nondestructive testing of spent nuclear fuel elements. Considering the difficulty of obtaining experimental data in a high-radiation environment and the characteristic of high noise of neutron images, it is difficult to use the traditional FBP algorithm to recover the complete information of the sample based on the limited projection data. Therefore, it is necessary to develop the sparse-view CT reconstruction algorithm for indirect neutron imaging. In order to improve the quality of the reconstruction image, an iterative reconstruction method combining SIRT, MRP, and WTDM regularization is proposed. The reconstruction results obtained by using the proposed method on simulated data and actual neutron projection data are compared with the results of four other algorithms (FBP, SIRT, SIRT-TV, and SIRT-WTDM). The experimental results show that the SIRT-MWTDM algorithm has great advantages in both objective evaluation index and subjective observation in the reconstruction image of simulated data and neutron projection data.
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Whitney, Darryl, David Klein, Tiffany Rodriguez, Lena Kenny, Eric Strauss, and Matthew Kingery. "Synovial Fluid Biomarkers in the Osteoarthritic Knee: Differences in Concentration with Progression of Radiographic Severity." Orthopaedic Journal of Sports Medicine 8, no. 7_suppl6 (July 1, 2020): 2325967120S0044. http://dx.doi.org/10.1177/2325967120s00446.
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Objectives: The purpose of the current study was to analyze the synovial fluid concentrations of known inflammatory biomarkers in the setting of symptomatic osteoarthritis (OA) of the knee and assess for any differences in biomarker concentrations based on the extent of radiographic disease. Methods: Patients presenting with knee complaints were invited to provide synovial fluid samples from the symptomatic knee during their initial office visit. For this study, a subset of patients with OA was analyzed. The concentration of 16 synovial fluid biomarkers was measured, including TIMP-1, TIMP-2, TIMP-3, MMP-13, IL-6, MCP-1, MIP-1β, VEGF, bFGF, eotaxin, IL-1Ra, MMP-1, MMP-3, MMP-9, RANTES, and TSG-6. Samples were analyzed using a multiplex magnetic bead immunoassay. Patients were divided into a low-grade OA group (K-L ≤ 2 or OARSI ≤ 1) or a high-grade cartilage OA group (K-L ≥ 3 or OARSI ≥ 2). Results: 101 patients were included in this analysis. There was a significant difference in MIP-1β (p=0.025) and bFGF (p=0.015) concentrations between OARSI grade groups. Patients with high-grade joint space narrowing had significantly greater concentrations of MIP-1β (p=0.022) and bFGF (p=0.003). There was a significant difference in MIP-1β concentration between K-L grade groups (p=0.013). Patients in the high-grade K-L group had a significantly greater concentration of MIP-1β (p=0.020). Conclusions: The synovial fluid concentrations of two synovial fluid biomarkers were found to differ significantly based on the extent of radiographic OA present. MIP-1β is a pro-inflammatory growth factor known to induce the synthesis of other inflammatory factors including interleukins and TNF-α. bFGF is a growth factor that is known to promote chondrogenesis, angiogenesis, wound healing, and granulation tissue formation. Continued study of synovial fluid biomarkers in the setting of symptomatic OA may improve our understanding of the pathogenesis of disease and identify treatment targets in an attempt to halt disease progression.
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Ogata, Ryouji, Nobuhiko Hiramatsu, Kunihiro Hayakawa, Shotaro Nakajima, Jian Yao, Tetsuro Kobayashi, and Masanori Kitamura. "Impairment of MCP-1 Expression in Mesothelial Cells Exposed to Peritoneal Dialysis Fluid by Osmotic Stress and Acidic Stress." Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis 31, no. 1 (January 2011): 80–89. http://dx.doi.org/10.3747/pdi.2009.00159.
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BackgroundBacterial peritonitis is one of the most frequent complications in patients on peritoneal dialysis. In the present study, we investigated effects of peritoneal dialysis fluid (PDF) on mesothelial cell recruitment of macrophages, focusing especially on unphysiological properties of PDF.MethodsHuman and murine mesothelial cells were exposed to PDF or individual properties of PDF (low pH, high glucose concentration, hyperosmolality, high lactate concentration) in vitro and in vivo, treated with inflammatory stimuli, and subjected to analyses of monocyte chemo-attractant protein-1 (MCP-1), nuclear factor-κB (NF-κB), mitogen-activated protein (MAP) kinases, and MAP kinase phosphatase-1 (MKP-1).ResultsWe found that intraperitoneal administration of PDF suppressed expression of MCP-1 and infiltration of mononuclear cells in the peritoneum of mice following injection with lipopolysaccharide. Among the unphysiological properties of PDF, low pH and hyperosmolality caused blunted induction of MCP-1 in cytokine-stimulated mesothelial cells. The attenuated response was ascribed to suppression of NF-κB by low pH and inhibition of p38 MAP kinase by hyperosmolality. Furthermore, the attenuated phosphorylation of p38 MAP kinase by osmotic stress was associated with induction of MKP-1.ConclusionThese results suggest a possibility that mesothelial cells exposed to PDF exhibit attenuated MCP-1 expression and consequent impairment of macrophage recruitment through dual mechanisms, that is, inhibition of NF-κB by acidic stress and blunted activation of p38 MAP kinase by osmotic stress. In patients on peritoneal dialysis, blunted expression of chemokines may lead to perturbation of bacterial clearance by macrophages in the peritoneal cavity.
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Clair, Andrew J., Matthew T. Kingery, Utkarsh Anil, Lena Kenny, Thorsten Kirsch, and Eric J. Strauss. "Alterations in Synovial Fluid Biomarker Levels in Knees With Meniscal Injury as Compared With Asymptomatic Contralateral Knees." American Journal of Sports Medicine 47, no. 4 (February 20, 2019): 847–56. http://dx.doi.org/10.1177/0363546519825498.
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Background: Changes in the joint microenvironment after an injury to the articular surface of the knee have been implicated in the pathogenesis of osteoarthritis. While prior studies focused on changes in this microenvironment after anterior cruciate ligament ruptures, few have explored the biomarker changes that occur in the setting of meniscal injuries. Purpose: To determine whether meniscal injury results in significant alterations to synovial fluid biomarker concentrations as compared with noninjured contralateral knees. Additionally, to explore the relationship between synovial fluid biomarkers and the degree of cartilage injury seen in these patients. Study Design: Cross-sectional study; Level of evidence, 3. Methods: Patients undergoing surgery for unilateral meniscal injury were prospectively enrolled from October 2011 to December 2016, forming a cohort that had synovial fluid samples collected from both the injured knee and the contralateral uninjured knee at the time of meniscal surgery. Synovial fluid samples were collected just before incision, and the concentrations of 10 biomarkers of interest were determined with a multiplex magnetic bead immunoassay. The concentrations of synovial fluid biomarkers from the operative and contralateral knees were compared. Additionally, the synovial fluid biomarker concentrations of operative knees from patients with associated high-grade cartilage lesions were compared with those with low-grade lesions. Results: The current analysis included synovial fluid samples from 82 knees (41 operative and 41 contralateral) from 41 patients undergoing arthroscopic surgery to treat a symptomatic meniscal injury. The mean ± SD age of patients was 49.86 ± 11.75 years. There were significantly greater concentrations of 4 of the 5 proinflammatory biomarkers (IL-6, MCP-1, MIP-1β, and MMP-3) in symptomatic knees as compared with asymptomatic knees when controlling for the duration of symptoms, body mass index, age, and the random effects of by-patient variability. In the injured knees, associated high-grade cartilage lesions were predictive of elevated MCP-1, MIP-1β, and VEGF levels. Low synovial fluid concentration of TIMP-1 or a greater ratio of MMP-3 to TIMP-1 was associated with the presence of synovitis. Increasing age was found to be an independent predictor of increased IL-6, MCP-1, and VEGF concentrations in the setting of symptomatic meniscal injury. Conclusion: The authors identified 4 proinflammatory synovial fluid biomarkers whose concentrations were significantly different after meniscal injury as compared with uninjured contralateral knees. Furthermore, they describe the effects of associated cartilage damage, synovitis, and patient age on biomarker concentrations.
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Gallego-García, Diego, Sergio Gallego-García, and Manuel García-García. "An Optimized System to Reduce Procurement Risks and Stock-Outs: A Simulation Case Study for a Component Manufacturer." Applied Sciences 11, no. 21 (November 4, 2021): 10374. http://dx.doi.org/10.3390/app112110374.
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In the current global system; supply chains are at risk due to increasing procurement shortages, supply disruptions, and the reliability of on-time deliveries with the original order quantities. As a result, an anticipated management model is of vital importance to provide companies with the productive flexibility necessary to adapt quickly to supply changes, in order to ensure the quality and delivery time through efficient management of stocks and supply costs. In this context, this research aims to develop a system to complement classical procurement planning based on inventory management methods and MRP (material requirements planning) systems by considering suppliers’ behavior regarding procurement risks. For this purpose, a system is developed that seeks to simulate the impacts of procurement shortages of different natures. Moreover, the research investigates the development of a system that performs procurement planning of a component manufacturer to determine the supply orders necessary to meet the master production schedule. The system is analyzed based on a set of indicators in the event that the supplier of a material needed for production does not supply on time or has short-term problems. Several scenarios are simulated, and the results are quantified by changing the procurement order quantities, which may or may not follow the economic order quantity (EOQ) model, and the potential procurement disruptions or shortages. The results show how the simulation and anticipation of potential suppliers’ procurement behavior concerning potential shortages and their probability are key for successful procurement within a joint strategy with classical procurement methods.
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Antony, V. B., S. W. Godbey, S. L. Kunkel, J. W. Hott, D. L. Hartman, M. D. Burdick, and R. M. Strieter. "Recruitment of inflammatory cells to the pleural space. Chemotactic cytokines, IL-8, and monocyte chemotactic peptide-1 in human pleural fluids." Journal of Immunology 151, no. 12 (December 15, 1993): 7216–23. http://dx.doi.org/10.4049/jimmunol.151.12.7216.
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Abstract Pleural effusions secondary to various diseases are associated with the presence of different inflammatory cells. The role of selective chemotactic cytokines in the recruitment of phagocytes to the pleural space is unclear. IL-8 and monocyte chemotactic peptide-1 (MCP-1) are recently described cytokines that are chemotactic for neutrophils and monocytes, respectively. We prospectively studied 63 patients, using strictly defined criteria for their selection. IL-8 concentrations were elevated in both empyema fluid (9.15 +/- 0.89 ng/ml) and parapneumonic effusions (4.7 +/- 0.697 ng/ml) when compared with pleural effusions secondary to other diseases. IL-8 levels were higher in empyema fluid than in parapneumonic effusions (p = 0.01). There was a significant correlation between IL-8 levels and the total numbers of neutrophils in empyema fluids (r = 0.80). Chemotactic activity for neutrophils was elevated in empyema fluid and the addition of IL-8 neutralizing serum decreased bioactivity by 32.22%. Malignant pleural effusions had the highest levels of MCP-1 (12.0 +/- 3.7 ng/ml) when compared with others. Cytology-positive pleural fluids (n = 10) had a higher level of MCP-1 than cytology-negative effusions (p = < 0.05). Malignant pleural fluid MCP-1 levels correlated (r = 0.70) with the absolute number of monocytes in the pleural fluid. Neutralization of monocyte chemotactic activity of malignant pleural fluid by specific neutralizing serum caused a 70.3% inhibition of bioactivity. Immunohistochemical staining of malignant pleural fluid localized antigenic MCP-1 to malignant cells. We conclude that both IL-8 and MCP-1 play major but not exclusive roles in the recruitment of neutrophils and monocytes from the vascular compartment to the pleural space.
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Choi, Seung Ah, Saehim Ha, Hee-Young Lee, Joo Whan Kim, Jin Chul Paeng, and Ji Hoon Phi. "Abstract 2468: Overcoming the leptomeningeal seeding of medulloblastoma by targeting HSP70." Cancer Research 83, no. 7_Supplement (April 4, 2023): 2468. http://dx.doi.org/10.1158/1538-7445.am2023-2468.
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Abstract Background: Medulloblastoma (MB) is the most common malignant pediatric brain tumor and the development of leptomeningeal dissemination (LM seeding) through the cerebrospinal fluid (CSF) circulation is a major cause of treatment failure. We have previously established the MB LM seeding mouse model. In this study, we aimed to determine molecules that can target seeding (metastatic) cells by analyzing their specific genetic and functional characteristics. Methods: Seeding and non-seeding cells were isolated and cultured, respectively, and microarray analysis was carried out. Proliferation and wound-healing assays were performed through the HCS system, and viability, migration and adhesion assays were accomplished. In addition, the therapeutic effect of the selected inhibitor was analyzed by region of interest (ROI) analysis of bioluminescence in vivo. Results: The gene expression and functional characteristics of the successfully obtained seed cells and non-seed cells were compared and analyzed. Differentially expressed genes (DEGs) analysis indicated that heat shock protein 70 (Hsp70) was in the highest rank in seeding cells, and pathway enrichment analysis revealed that the metabolism was the main pathway enriched in seeding cells. Proliferation of seeding cells was slower than that of non-seeding cells, but there was no significant difference in viability. Notably, the wound-healing of seeding cells was delayed, but the adhesion ability on collagen was higher. Among the six HSP70 inhibitors, VER155008 was efficiently sensitive to seeding cells, and suppression of expression of MRP proteins along with HSP70 was also confirmed. In addition, it was observed that the treatment of VER155008 reduced seeding through the spinal cord in vivo. Conclusion: Our study suggests that VER15008 can be a promising treatment option, targeting LM seeding of MB. Citation Format: Seung Ah Choi, Saehim Ha, Hee-Young Lee, Joo Whan Kim, Jin Chul Paeng, Ji Hoon Phi. Overcoming the leptomeningeal seeding of medulloblastoma by targeting HSP70 [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 2468.
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Whitaker, John N. "Myelin basic protein in cerebrospinal fluid and other body fluids." Multiple Sclerosis Journal 4, no. 1 (February 1998): 16–21. http://dx.doi.org/10.1177/135245859800400105.
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Myelin basic protein (MBP) or a fragment thereof may enter cerebrospinal fluid (CSF) and other body fluids in an etiologically nonspecific fashion to provide information about the status of central nervous system (CNS) myelin damage. MBP immunochemically detected is referred to as MBP-like material (MBPLM). The clinical utility of the assay for MBPLM in CSF is to document the presence, continuation, or resolution of CNS myelin injury. The analysis of CSF for MBPLM is subject to many variables, among which are the antisera and the form of the assay utilized. The dominant epitope of CSF MBPLM is in the decapeptide of 80-89 from the intact MBP molecule of 170 residues. Normally, CSF has no detected MBPLM. Following an acute relapse of MS, MBPLM rises quickly in the range of ng/ml and rapidly declines and disappears. The presence of MBPLM in CSF in chronic and progressive phases of the disease is unusual, but it may sometimes be detected in low levels, depending on the assay used for detection. The level of CSF MBPLM is related to both the mass of CNS myelin damage and how recently it occurred. The level of CSF MBPLM rarely is elevated in optic neuritis. The level of CSF MBPLM is unrelated to CSF protein level, level of IgG, presence of oligoclonal bands or pleocytosis. CSF MBPLM has the potential of serving as a marker of therapeutic effectiveness in MS and does have predictive value for response to glucocorticoids given for worsening of disease. The detection of MBPLM in body fluids other than CSF would be of great value because of the resulting improved feasibility for objectively monitoring the natural history of MS and response to therapy. Studies on blood have yet to produce a valid assay of MBPLM. Urinary MBPLM, though different in its features from that in CSF, may provide a correlate, not with acute demyelination in MS as is the case for CSF, but with progression of disease.
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Neroev, V. V., N. V. Balatskaya, A. Yu Novikova, M. V. Ryabina, and P. A. Ilyukhin. "Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma." Medical Immunology (Russia) 22, no. 5 (December 1, 2020): 943–56. http://dx.doi.org/10.15789/1563-0625-pal-2002.
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Pathogenesis of retinal capillary hemangioma has not been sufficiently studied at the present time. Therefore, the study of cytokine levels in biological fluids seems to be very relevant in order to increase knowledge about the mechanisms of the disease development and searching for targeted therapies. The content of hematopoietic and vasoactive growth factors in blood serum, lacrimal fluid, and vitreous body was studied in patients with retinal capillary hemangioma. A total of 26 patients with retinal angiomatosis were examined. The samples of blood serum (n = 23) and lacrimal fluid (n = 10) from practically healthy people aged 22 to 46 (27.4±1.4 years) were used as a control. To perform comparative assessment of cytokine concentrations in the vitreous body of patients with retinal capillary hemangioma, were used samples of the vitreous body from 6 patients (average age 33±4.7 years; from 21 to 49 years) with rhegmatogenous retinal detachment. To measure the cytokine concentrations, we applied multiplex analysis technique using the xMAP platform with LuminexxPONENT 3.1 program and ProcartaPlex sets (eBioscience, Austria). A detailed characteristic of vasoactive factors in capillary retinal hemangioma was obtained as a result of this work. Some disorders in chemokine regulation were identified. There was a significant increase in serum concentrations of three vasoactive factors, i.e., PDGF-BB, HGF, and PIGF-1, with a decrease in chemokines (MCP-1, MIP-1α, and MIP-1β). The frequencies of PIGF-1 and MIP-1α detection also significantly differed from the control group. SCF was significantly more often determined in patients with retinal angiomatosis only at the systemic level. Correlations between PDGF-BB and PIGF-1, as well as PIGF-1 and MIP-1β were shown. A significant increase in VEGF-A, HGF, VEGF-D, as well as MCP-1 concentrations was shown in the lacrimal fluid. The inversion of PDGF-BB concentrations in serum and lacrimal fluid was noted. Analysis of intraocular cytokine levels revealed a significant increase in VEGF-A and HGF concentrations, with marked decrease in MIP-1α and MIP-1β. PDGF-BB in 100% of cases was determined only in vitreous body of patients with retinal angiomatosis. With respect to the revealed characteristic shifts of HGF/SF intraocular production in retinal capillary hemangioma, it seems relevant to search ways for its inhibition, thus providing potential basis for a new therapeutic strategy in treatment of retinal angiomatosis.
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Wang, Xiaolin, Ken Kamrin, and Chris H. Rycroft. "An incompressible Eulerian method for fluid–structure interaction with mixed soft and rigid solids." Physics of Fluids 34, no. 3 (March 2022): 033604. http://dx.doi.org/10.1063/5.0082233.
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We present a general simulation approach for incompressible fluid–structure interactions in a fully Eulerian framework using the reference map technique. The approach is suitable for modeling one or more rigid or finitely deformable objects or soft objects with rigid components interacting with the fluid and with each other. It is also extended to control the kinematics of structures in fluids. The model is based on our previous Eulerian fluid–soft solver [Rycroft et al., “Reference map technique for incompressible fluid–structure interaction,” J. Fluid Mech. 898, A9 (2020)] and generalized to rigid structures by constraining the deformation-rate tensor in a projection framework. Several numerical examples are presented to illustrate the capability of the method.
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Zheng, Yaoyang. "The Most Suitable Clearance in MCF (Magnetic Compound Fluid) Polishing." Journal of Advance Research in Mechanical & Civil Engineering (ISSN: 2208-2379) 3, no. 3 (March 31, 2016): 13–18. http://dx.doi.org/10.53555/nnmce.v3i3.322.
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Prof. Shimada has demonstrated experimentally that the polishing effect using our developed intelligent fluid, magnetic compound fluid (MCF), is greater than that using previously developed intelligent fluids, i.e., magnetic fluid (MF) and magneto-rheological fluid (MRF). Shimada succeeded in float polishing with a large clearance utilizing a newly developed magnetic responsive fluid, MCF, which was improved by the addition of a-cellulose, thereby achieving a clearance as great as 8mm. He also clarified the mechanism of the new polishing technique. A comparison of the MCF polishing effect with those of techniques using the ordinary magnetic responsive fluids, MF and MRF, showed the polishing effect of MCF to be greater than those of MF and MRF. The MCF polishing technique is applicable to many types of polishing, widening the variety of the uses of magnetic float polishing (MFP). However, while clearance to8mm is possible, this may not be the most suitable value. In this study, we clarified the most suitable value for MCF abrasion experimentally.
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Yoshimura, T., and E. J. Leonard. "Secretion by human fibroblasts of monocyte chemoattractant protein-1, the product of gene JE." Journal of Immunology 144, no. 6 (March 15, 1990): 2377–83. http://dx.doi.org/10.4049/jimmunol.144.6.2377.
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Abstract We recently purified human monocyte chemoattractant protein-1 (MCP-1) from culture fluids of either human glioma cell lines or mitogen-stimulated human peripheral blood mononuclear leukocytes. It has now been shown that MCP-1 is the product of the gene JE, which was first recognized by its expression in fibroblasts stimulated with platelet-derived growth factor (PDGF). We therefore studied secretion of MCP-1 by three human fibroblast cell lines. Monocyte chemotactic activity was found in culture fluids of all three lines after growth to confluence in DMEM-10% FCS, and the amounts secreted per cell were comparable for the three lines. The MRC-5 line was chosen for further study. Monocyte chemotactic activity secretion by confluent MRC-5 cultures continued after a switch to serum-free medium and was not inhibited by anti-PDGF antibody, indicating that secretion may not have been caused by autocrine release of PDGF. When concentrated serum-free MRC-5 culture fluid was injected into an HPLC gel filtration column, only one chemotactic activity peak was observed, which was in the same location as glioma-derived MCP-1. The activity was completely absorbed out by an anti-MCP-1 affinity column, which indicates that all the chemotactic activity in MRC-5 culture fluid was accounted for by MCP-1. PDGF caused a marked increase in chemotactic activity over that found in serum-free culture fluid of MRC-5 or 501T cells. Immunoprecipitation by anti-human MCP-1 showed two bands, corresponding to the two forms of MCP-1 previously described (MCP-1 alpha and beta); and the amounts increased in response to PDGF stimulation. Thus, the reported increase in human fibroblast JE mRNA in response to PDGF-containing serum stimulation is reflected in increased secretion of the MCP-1 gene product.
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Gopichandran, Nadia, Uma V. Ekbote, James J. Walker, David Brooke, and Nicolas M. Orsi. "Multiplex determination of murine seminal fluid cytokine profiles." Reproduction 131, no. 3 (March 2006): 613–21. http://dx.doi.org/10.1530/rep.1.00959.
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Seminal fluid is known to be responsible for orchestrating mating-induced immunomodulation. Central to this process are numerous cytokines that modulate uterine leukocyte recruitment and trafficking. Despite this, a comprehensive analysis of the cytokine profile of murine seminal fluid is lacking. This study addressed this issue by using multiplex immunoassays to characterise the profile of interleukin (IL)-1α , IL-1β , IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-17, eotaxin, granulocyte-colony stimulating factor (G-CSF), granulocyte macrophage-colony stimulating factor (GM-CSF), interferon (IFN)-γ, keratinocyte-derived chemokine (KC), monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-1α , MIP-1β , regulated upon activation normal T-cell expressed and secreted (RANTES), and tumour necrosis factor (TNF)-α in fluid drawn from the seminal vesicles of single mice (n = 18). Their levels and ratios were compared with those found in serum. IL-1α , IL-1β , IL-2, IL-5, IL-9, IL-12 (p40), IL-12 (p70), IL-13, IL-17, GM-CSF, IFN-γ, MCP-1 and TNF-α levels were significantly higher in serum; IL-4, G-CSF, eotaxin, KC and RANTES exhibited the opposite trend. Based on these findings, we propose a model of mating-induced immunomodulation that implicates seminal eotaxin, RANTES and MIP-1α in the relocation and concentration of extravasated migrating endometrial eosinophils to the luminal epithelium. Furthermore, KC may participate in uterine neutrophil chemotaxis and activation. Eotaxin and MIP-α , together with IL-1β and IL-9, may also enhance further cytokine synthesis for endometrial antigen-presenting cell recruitment for processing paternal ejaculate antigens. IL-4 and G-CSF could also minimise deleterious cell-mediated immunity and modulate IFN-γ production, thereby supporting the establishment of pregnancy.
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Sidiq, Rendi, Bastian Lubis, and Yutu Solihat. "Comparison of Pre-Loading Fluid With Norepinephrine Toward Mean Arterial Pressure (MAP) In Sepsis Patients In Intensive Care Unit (ICU) of Haji Adam Malik General Hospital, Medan." Journal of Society Medicine 1, no. 2 (November 30, 2022): 36–43. http://dx.doi.org/10.47353/jsocmed.v1i2.7.
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Introduction: Sepsis is a life-threatening organ dysfunction caused by dysregulation of the host response to infection. Sepsis and septic shock are major health problems, affecting millions of people worldwide each year and killing one in six people affected. Early identification and appropriate management in the early hours after the development of sepsis improves the patient's prognosis. Surviving Sepsis Campaign (SSC) 2021 recommends a fluid dose of 30mL/kgBW, but there are many studies stating that there is no difference in patient outcomes when we resuscitate patients with 10 or 20 mL/kgBW fluids. Norepinephrine is considered as the safest and most potent vasopressor agents than others.
Methods: This study used a double-blind randomized clinical trial (RCT) design to assess MAP in sepsis patients in intensive care unit (ICU) of Haji Adam Malik General Hospital ,Medan.
Results: There were more male (56.5%), than female (43.5%). In our study, administration of 10mL/kg and 20mL/kg fluid bolus with vasopressor resulted in increased MAP, and the differences were statistically significant (p < 0.05). Administration of 10 mL/kg fluid gave higher MAP values than the other groups, could be a consideration in choosing fluid in order to avoid fluid overload.
Conclusion: There is a significant comparison in the ratio of norepinephrine pre-loading fluid toward MAP in sepsis patients. Comparison of the mean MAP value at 15, 20, 25 minutes was the highest in the 10 mL/KgBW group. Meanwhile, the lowest MAP was found in 30mL/KgBW the group.
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Capelli, A., A. Di Stefano, I. Gnemmi, P. Balbo, C. G. Cerutti, B. Balbi, M. Lusuardi, and C. F. Donner. "Increased MCP-1 and MIP-1β in bronchoalveolar lavage fluid of chronic bronchitics." European Respiratory Journal 14, no. 1 (July 1999): 160. http://dx.doi.org/10.1034/j.1399-3003.1999.14a27.x.
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WANG, LINXIANG, YURUN FAN, and YING CHEN. "ANIMATION OF CHAOTIC MIXING BY A BACKWARD POINCARE CELL-MAP METHOD." International Journal of Bifurcation and Chaos 11, no. 07 (July 2001): 1953–60. http://dx.doi.org/10.1142/s0218127401003139.
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A Backward Poincare cell-mapping (BPCM) method has been developed for animating chaotic fluid mixing. The chaotic mixing field considered is induced by periodically rotating the secondary flow of incompressible fluids in a curved pipe. The pipe's cross-section is transformed into a cell space where each cell is initially assigned with a color code and mapped by integrating the velocity field forward in time. The mixing process is thus animated efficiently with each cell being painted with its color on a computer screen. We propose the backward Poincare cell-mapping instead of direct Poincare cell-mapping as a useful tool for probing the chaotic fluid mixing and for animating the phase deformation of nonlinear dynamical systems.
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Huffnagle, G. B., R. M. Strieter, L. K. McNeil, R. A. McDonald, M. D. Burdick, S. L. Kunkel, and G. B. Toews. "Macrophage inflammatory protein-1alpha (MIP-1alpha) is required for the efferent phase of pulmonary cell-mediated immunity to a Cryptococcus neoformans infection." Journal of Immunology 159, no. 1 (July 1, 1997): 318–27. http://dx.doi.org/10.4049/jimmunol.159.1.318.
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Abstract Our objective was to define the role of the chemokine macrophage-inflammatory protein-1alpha (MIP-1alpha) in the efferent phase of pulmonary cell-mediated immunity (CMI) against Cryptococcus neoformans. Following intratracheal inoculation of C. neoformans (24067) into CBA/J mice, the development of CMI was required for leukocyte recruitment into the lungs at 2 wk postinfection. MIP-1alpha mRNA was expressed by day 6 postinfection, and MIP-1alpha protein in bronchoalveolar lavage fluid was detectable at day 6, but significantly elevated at days 19 and 33. Administration of neutralizing anti-MIP-1alpha Abs from days 7 to 13 blocked the increase in bronchoalveolar lavage fluid MIP-1alpha and resulted in a 37% decrease in total leukocytes in the lungs at day 16. There were 66% fewer macrophages/monocytes and 42% fewer neutrophils in the lungs of anti-MIP-1alpha-treated mice, and the pulmonary burden of C. neoformans was threefold higher. There was no significant difference in the number of eosinophils, CD4+, CD8+, or B220+ lymphocytes between the two groups of mice. Neutralization of MIP-1alpha did not significantly decrease the levels of monocyte chemotactic protein-1 (MCP-1); however, neutralization of MCP-1 significantly decreased MIP-1alpha levels, demonstrating that induction of MIP-1alpha was largely dependent on MCP-1 production. Neutralization of MIP-1alpha also blocked the cellular recruitment phase of a recall response to cryptococcal Ag in the lungs of immunized mice. Thus, in both the contexts of active cryptococcal infection or rechallenge with cryptococcal Ag, MIP-1alpha was required during the efferent phase of CMI for maximal leukocyte recruitment into the lungs, most notably the recruitment of phagocytic effector cells (neutrophils and macrophages).
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Imre, Attila, Réka Kustán, and Axel Groniewsky. "Mapping of the Temperature–Entropy Diagrams of van der Waals Fluids." Energies 13, no. 6 (March 23, 2020): 1519. http://dx.doi.org/10.3390/en13061519.
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The shape of the temperature vs. specific entropy diagram of a working fluid is very important to understanding the behavior of fluid during the expansion phase of the organic Rankine cycle or similar processes. Traditional wet-dry-isentropic classifications of these materials are not sufficient; several materials remain unclassified or misclassified, while materials listed in the same class might show crucial differences. A novel classification, based on the characteristic points of the T–s diagrams was introduced recently, listing eight different classes. In this paper, we present a map of these classes for a model material, namely, the van der Waals fluid in reduced temperature (i.e., reduced molecular degree of freedom) space; the latter quantity is related to the molar isochoric specific heat. Although van der Waals fluid cannot be used to predict material properties quantitatively, the model gives a very good and proper qualitative description. Using this map, some peculiarities related to T–s diagrams of working fluids can be understood.
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Al-Majid, Ahmed, Saeed Alassiri, Nilminie Rathnayake, Taina Tervahartiala, Dirk-Rolf Gieselmann, and Timo Sorsa. "Matrix Metalloproteinase-8 as an Inflammatory and Prevention Biomarker in Periodontal and Peri-Implant Diseases." International Journal of Dentistry 2018 (September 16, 2018): 1–27. http://dx.doi.org/10.1155/2018/7891323.
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Levels of and especially the degree of activation of matrix metalloproteinase (MMP-8) in oral fluids (i.e., saliva, mouth rinse, gingival crevicular fluid (GCF) and peri-implantitis sulcular fluid (PISF)) increase to pathologically elevated levels in the periodontal and peri-implant diseases. This study aimed at collecting and collating data from previously published studies and determining whether active MMP-8 (aMMP-8) could serve as a biomarker for the diagnosis and prevention of periodontal and peri-implant diseases. The literature search identified a total of 284 articles. Out of 284 articles, 61 articles were found to be relevant. Data obtained from the selected studies were combined, and it indicated that aMMP-8 in oral fluids exerts the strong potential to serve as a useful adjunctive diagnostic and preventive biotechnological tool in periodontal and peri-implant diseases. aMMP-8 can be used alone or in combination with other proinflammatory and/or microbiological biomarkers.
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Takahashi, Shizuka, Kobu Adachi, Yukihiko Suzuki, Atsuko Maeno, and Mitsuru Nakazawa. "Profiles of Inflammatory Cytokines in the Vitreous Fluid from Patients with Rhegmatogenous Retinal Detachment and Their Correlations with Clinical Features." BioMed Research International 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/4256183.
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Purpose. To characterize the profiles for inflammatory cytokines in the vitreous fluid from patients with rhegmatogenous retinal detachment (RRD) by comparing those of other vitreoretinal diseases and to analyze the correlation between intravitreal cytokines and clinical features.Materials and Methods. Vitreous fluid was obtained at the time of surgery from 28 RRD eyes. Vitreous fluid was similarly collected from patients with macular hole (MH), epiretinal membrane, proliferative diabetic retinopathy (PDR), and retinal vein occlusion as controls. Twenty-seven cytokines were measured. Intravitreal cytokine profiles in RRD were characterized by comparing these with those in other vitreoretinal diseases. We also analyzed the correlations between vitreous cytokines and clinical features.Results. There were statistical differences in the MCP-1, MIP-1β, and IP-10 between the RRD and MH, while the IL-6 and IL-8 exhibited levels that were between those for the PDR and MH. MIP-1βwas significantly correlated to both the extent and duration of the RRD, while IL-8 was significantly correlated to the extent of the RRD.Conclusions. MCP-1, MIP-1β, and IP-10 may modify the pathologic features of RRD. The levels of these cytokines are related in part to the clinical features and the level of photoreceptor damage.
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Zhao, Qiyu, Leigh G. Simpson, Kevin E. Driscoll, and George D. Leikauf. "Chemokine regulation of ozone-induced neutrophil and monocyte inflammation." American Journal of Physiology-Lung Cellular and Molecular Physiology 274, no. 1 (January 1, 1998): L39—L46. http://dx.doi.org/10.1152/ajplung.1998.274.1.l39.
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Pulmonary inflammation has been observed in humans and in many animal species after ozone exposure. Inflammatory cell accumulation involves local synthesis of chemokines, including neutrophil chemoattractants such as macrophage inflammatory protein-2 (MIP-2), and monocyte chemoattractants, such as monocyte chemoattractant protein-1 (MCP-1). To better understand the mechanism of ozone-induced inflammation, we exposed mice and rats to ozone for 3 h and measured MIP-2 and MCP-1 gene expression. In C57BL/6 mice, steady-state mRNA levels for MCP-1 in the lung increased at 0.6 parts/million (ppm) ozone and were maximal at 2.0 ppm ozone. After exposure to 2 ppm ozone, MIP-2 mRNA levels peaked at 4 h postexposure, whereas MCP-1 mRNA levels peaked at 24 h postexposure. Neutrophils and monocytes recovered in bronchoalveolar lavage fluid peaked at 24 and 72 h, respectively. The accumulation of monocytes was thus delayed relative to that of neutrophils, consistent with the sequential expression of the corresponding chemokines. The role of MCP-1 in monocyte accumulation was evaluated in greater detail in rats. Ozone caused an increase in monocyte chemotactic activity in bronchoalveolar fluid that was inhibited by an antibody directed against MCP-1. Ozone-induced MCP-1 mRNA levels were higher in lavage cells than in whole lung tissue, indicating that lavage cells are an important source of MCP-1. In these cells, nuclear factor-κB, a nuclear transcription factor implicated in MCP-1 gene regulation, was also activated 20–24 h after ozone exposure. These findings indicate that monocyte accumulation subsequent to acute lung injury can be mediated through MCP-1 and that nuclear factor-κB may play a role in ozone-induced MCP-1 gene expression.
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AKAGAMI, Y., and M. MURAOKA. "MEP-01: A Novel Polishing Method using Abrasive Dispersion Typed Functional Fluid(MEP-I: MECHANICAL PROCECCING)." Proceedings of the JSME Materials and Processing Conference (M&P) 2005 (2005): 7. http://dx.doi.org/10.1299/jsmeintmp.2005.7_3.
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Heijmans-Antonissen, Claudia, Feikje Wesseldijk, Renate JM Munnikes, Frank JPM Huygen, Patrick van der Meijden, Wim C. J. Hop, Herbert Hooijkaas, and Freek J. Zijlstra. "Multiplex Bead Array Assay for Detection of 25 Soluble Cytokines in Blister Fluid of Patients with Complex Regional Pain Syndrome Type 1." Mediators of Inflammation 2006 (2006): 1–8. http://dx.doi.org/10.1155/mi/2006/28398.
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Inflammatory processes are known to be involved at least in the early phase of complex regional pain syndrome type 1 (CRPS1). Blister fluid obtained from the involved extremities displayed increased amounts of proinflammatory cytokines IL-6 and TNFαcompared with the noninvolved extremities. The aim of this paper is to investigate the involvement of mediators by measurement of several other cytokines using new detection techniques that enable multiple cytokine measurement in small samples. The use of a multiplex-25 bead array cytokine assay and Luminex technology enabled simultaneous measurement of representative (1) proinflammatory cytokines such as GM-CSF, IL-1β, IL-1RA, IL-6, IL-8, and TNF-α; (2) Th1/Th2 distinguishing cytokines IFN-γ, IL-2, IL-2R, IL-4, IL-5, and IL-10; (3) nonspecific acting cytokines IFN-α, IL-7, IL-12p40/p70, IL-13, IL-15, and IL-17; and (4) chemokines eotaxin, IP-10, MCP-1, MIP-1α, MIP-1β, MIG, and RANTES. Although minimal detection levels are significantly higher in the bead array system than those in common ELISA assays, in blister fluid, IL-1RA, IL-6, IL-8, TNF-α, IL-12p40/p70, MCP-1, and MIP-1βwere detectable and increased in CRPS1 affected extremities. Levels of IL-6 and TNF-αsimultaneously measured by ELISA (Sanquin Compact kit) and by multiplex-25 bead array assay (Biosource) were highly correlated (r=0.85,P<.001for IL-6 andr=0.88,P<.001for TNF-α). Furthermore, IP-10 and eotaxin were detectable but diminished in CRPS1, whereas detectable amounts of IL-10 were similar in involved and noninvolved extremities. Multiplex bead array assays are useful systems to establish the involvement of cytokines in inflammatory processes by measurements in blister fluids of CRPS1. Ten representative cytokines were detectable. However, detection levels and amounts measured are at least 3 times higher in the multiplex-25 array assay than in the ELISA assays used simultaneously for the measurement of cytokines.
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Karzai, W., J. M. Reilly, W. D. Hoffman, R. E. Cunnion, R. L. Danner, S. M. Banks, J. E. Parrillo, and C. Natanson. "Hemodynamic effects of dopamine, norepinephrine, and fluids in a dog model of sepsis." American Journal of Physiology-Heart and Circulatory Physiology 268, no. 2 (February 1, 1995): H692—H702. http://dx.doi.org/10.1152/ajpheart.1995.268.2.h692.
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To study how sepsis affects hemodynamic responses to catecholamines and fluids, either Escherichia coli-infected (septic, n = 8) or sterile (controls, n = 6) fibrin clots were implanted intraperitoneally into 2-yr-old beagles. Hemodynamics were measured at each of four doses of dopamine (0, 5, 10, and 20 micrograms.kg-1.min-1) and norepinephrine (0, 10, 20, and 40 micrograms.min-1), before and after infusion of fluid (Ringer 40 ml.kg-1). Septic animals had lower mean arterial pressure (MAP, P = 0.04), stroke volume index (SVI, P = 0.0001), and left ventricular (LV) ejection fraction (LVEF) (P = 0.0001) than controls. During this time, increasing doses of dopamine and norepinephrine produced corresponding increases (P < 0.001) in LVEF, SVI, and MAP. However, during sepsis, the ability of dopamine to increase MAP diminished, while its ability to increase LVEF and SVI was maintained. Conversely, the ability of norepinephrine to increase LVEF and SVI diminished, but its ability to increase MAP was maintained. During sepsis, fluids alone increased (P < 0.05) MAP, LVEF, SVI, and cardiac index (CI). Fluids with catecholamines also significantly increased (P < 0.05) MAP with only minimal increases in LVEF, SVI, and CI. These data demonstrate that during sepsis without catecholamines, fluids improve cardiac performance and systemic pressures, but with catecholamines, fluids have minimal effects on cardiac performance and augment MAP. Furthermore, during sepsis dopamine is more effective than norepinephrine in increasing LV performance, but norepinephrine is more effective than dopamine in increasing systemic pressures.
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Evans, C. R., and K. L. Johnson. "Regimes of Traction in Elastohydrodynamic Lubrication." Proceedings of the Institution of Mechanical Engineers, Part C: Journal of Mechanical Engineering Science 200, no. 5 (September 1986): 313–24. http://dx.doi.org/10.1243/pime_proc_1986_200_135_02.
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The rheological behaviour of the lubricant in an elastohydrodynamic contact depends upon the properties of the fluid and the imposed conditions of load, speed and temperature. For a lubricant of known rheological properties, it is shown how a ‘map’ can be constructed in which different areas of the map correspond to different regimes of behaviour. The coordinates chosen for the map are non-dimensional pressure and a parameter which is closely related to the film thickness. Given the load, speed and temperature, the operating conditions can be located as a point in the map. Maps have been constructed for three fluids: a mineral oil, polyphenyl ether 5P4E and a traction fluid. In general the maps display four regimes: (a) Newtonian, (b) Eyring, (c) viscoelastic and (d) elastic-plastic. Appropriate constitutive equations are proposed for each regime from which the traction forces can be predicted. The maximum traction coefficients calculated in this way are well supported by experiment.
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Wang, Boli, Xisen Zhao, Wen Zhou, Bin Chang, and Hao Xu. "Quantitative Characterization of Pore Connectivity and Movable Fluid Distribution of Tight Sandstones: A Case Study of the Upper Triassic Chang 7 Member, Yanchang Formation in Ordos Basin, China." Geofluids 2020 (January 23, 2020): 1–13. http://dx.doi.org/10.1155/2020/5295490.
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The pore connectivity and distribution of moveable fluids, which determines fluid movability and recoverable reserves, are critical for enhancing oil/gas recovery in tight sandstone reservoirs. In this paper, multiple techniques including high-pressure mercury intrusion porosimetry (MIP), nuclear magnetic resonance (NMR), scanning electron microscopy (SEM), and microcomputer tomography scanning (micro-CT) were used for the quantitative characterization of pore structure, pore connectivity, and movable fluid distribution. Firstly, sample porosity and permeability were obtained. Pore morphology and the 3D distribution of the pore structures were analyzed using SEM and micro-CT, respectively. The pore-size distribution (PSD) from NMR was generally broader than that from MIP because this technique simply characterized the connected pore volume, whereas NMR showed the total pore volume. Therefore, an attempt was made to calculate pore connectivity percentages of pores with different radii (<50 nm, 50 nm–0.1 μm, and 0.1 μm–1 μm) using the difference between the PSD obtained from MIP and NMR. It was found that small pores (r<0.05 μm) contributed 5.02%–18.00% to connectivity, which is less than large pores (r>0.05 μm) with contribution of 36.60%–92.00%, although small pores had greater pore volumes. In addition, a new parameter, effective movable fluid saturation, was proposed based on the initial movable fluid saturation from NMR and the pore connectivity percentage from MIP and NMR. The results demonstrated that the initial movable fluid saturation decreased by 14.16% on average when disconnected pores were excluded. It was concluded that the effective movable fluid saturation has a higher accuracy in evaluating the recovery of tight sandstone reservoirs.
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Simpson, Joelle N., and Stephen J. Teach. "Pediatric rapid fluid resuscitation." Current Opinion in Pediatrics 23, no. 3 (June 2011): 286–92. http://dx.doi.org/10.1097/mop.0b013e3283460599.
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SANDY, John D., and Christie VERSCHAREN. "Analysis of aggrecan in human knee cartilage and synovial fluid indicates that aggrecanase (ADAMTS) activity is responsible for the catabolic turnover and loss of whole aggrecan whereas other protease activity is required for C-terminal processing in vivo." Biochemical Journal 358, no. 3 (September 10, 2001): 615–26. http://dx.doi.org/10.1042/bj3580615.
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Studies of aggrecan proteolysis in human joints have implicated both the aggrecanase [ADAMTS, a disintegrin-like and metalloprotease (reprolysin-type) with thrombospondin type 1 motif] and matrix metalloproteinase (MMP) families. We have analysed the aggrecan core protein species present in vivo in both articular cartilage and synovial fluids from normal, acutely injured and osteoarthritic joints. Normal cartilage contains at least seven major G1 domain (the N-terminal globular domain of aggrecan)-bearing species, of which three (full-length core, G1-NITEGE373 and G1-VDIPEN341) have been identified. The C-terminals of the others are unknown but digestion of fetal human aggrecan with MMP-3 and crude aggrecanase suggests that they are products of MMP-like activity in vivo. Normal synovial fluids contain at least 10 species, of which nine result from ADAMTS-dependent cleavage, and this cleavage occurs at all of the five known aggrecanase sites. Aggrecan fragments in the cartilage and synovial fluids of acutely injured joints are generally similar to normal, but all contain a markedly increased ratio of G1-NITEGE to G1-VDIPEN. Aggrecan from the cartilage of late-stage osteoarthritis patients is remarkably similar to normal, whereas the synovial fluid aggrecan is more fragmented than that from normal or injured knees. The analyses suggest that the role of the ADAMTS and these MMP-like activities in human cartilage are distinctly different. Excessive ADAMTS activity in vivo is destructive to cartilage matrix, since the bulk of the glycosaminoglycan (GAG)-bearing products are released from the tissue into the synovial fluid following cleavage of the Glu373–Ala374 bond. In contrast, the MMP-like activity appears to be essentially non-destructive, since much of the GAG-bearing product is retained in the tissue following cleavages that are in the more C-terminal regions of the molecule.
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Cai, Mingyu, Yuliang Su, Lei Li, Yongmao Hao, and Xiaogang Gao. "CO2-Fluid-Rock Interactions and the Coupled Geomechanical Response during CCUS Processes in Unconventional Reservoirs." Geofluids 2021 (February 26, 2021): 1–22. http://dx.doi.org/10.1155/2021/6671871.
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The difficulty of deploying remaining oil from unconventional reservoirs and the increasing CO2 emissions has prompted researchers to delve into carbon emissions through Carbon Capture, Utilization, and Storage (CCUS) technologies. Under the confinement of nanopore in unconventional formation, CO2 and hydrocarbon molecules show different density distribution from in the bulk phase, which leads to a unique phase state and interface behavior that affects fluid migration. At the same time, mineral reactions, asphaltene deposition, and CO2 pressurization will cause the change of porous media geometry, which will affect the multiphase flow. This review highlights the physical and chemical effects of CO2 injection into unconventional reservoirs containing a large number of micro-nanopores. The interactions between CO2 and in situ fluids and the resulting unique fluid phase behavior, gas-liquid equilibrium calculation, CO2 adsorption/desorption, interfacial tension, and minimum miscible pressure (MMP) are reviewed. The pore structure changes and stress distribution caused by the interactions between CO2, in situ fluids, and rock surface are discussed. The experimental and theoretical approaches of these fluid-fluid and fluid-solid reactions are summarized. Besides, deficiencies in the application and safety assessment of CCUS in unconventional reservoirs are described, which will help improve the design and operation of CCUS.
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Yuan, Hua, Russell T. Johns, Azubuike M. Egwuenu, and Birol Dindoruk. "Improved MMP Correlation for CO2 Floods Using Analytical Theory." SPE Reservoir Evaluation & Engineering 8, no. 05 (October 1, 2005): 418–25. http://dx.doi.org/10.2118/89359-pa.
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Summary Local displacement efficiency from CO2 gas injection is highly dependent on the minimum miscibility pressure (MMP). Correlations are sometimes used to estimate the MMP where the injected fluid may or may not contain impurities such as methane. These correlations, however, are based on a limited set of experimental data and, as such, are not widely applicable. They also do not account accurately for the more complex condensing/vaporizing (CV) displacement process. This paper presents new MMP correlations for the displacement of multicomponent oil by CO2 and impure CO2. The approach is to use recently developed analytical theory for MMP calculations from equations of state (EOSs)to generate MMP correlations for displacements by pure and impure CO2. The advantage of this approach is that MMPs for a wide range of temperatures and reservoir fluids can be calculated quickly and accurately without introducing uncertainties associated with slimtube MMPs and other numerical methods. The improved MMP correlations are based solely on the reservoir temperature, the molecular weight of C7+, and the percentage of intermediates (C2-C6) in the oil. The MMPs from the improved correlations are compared to currently used correlations and 41 experimentally measured MMPs. Correlations are also developed for impure-CO2 floods, in which the injection stream may contain upto 40% methane. The new correlations are more accurate for a wider range of conditions than the currently used correlations. Introduction Whorton et al. received a patent in 1952 to improve oil recovery by the injection of CO2. CO2 injection has been ongoing ever since, primarily because CO2 develops multicontact miscibility (MCM) with reservoir fluids at low pressures. There are also potential environmental benefits of CO2 injection in that subsurface sequestration of greenhouse gases has become an important U.S. priority. The MMP is an important optimization parameter in CO2 floods. Recoveries from slimtube experiments often give a slope change at the MMP. Above the MMP, slimtube recoveries (or local displacement efficiencies) typically do not increase significantly with enrichment. Thus, the accurate determination of MMP is important in gasflood design. Pseudoternary diagrams traditionally have been used to explain the behavior of multicontact miscible (MCM) gas-drive processes. Real oil displacements by CO2, however, have recently been shown to have features of both vaporizing and condensing drives. The 2D nature of pseudoternary diagrams often leads to incorrect interpretations, especially for CV drives. Analytical theory has no such restrictions and can be applied for any number of components. The CV process greatly complicates the accurate estimation of MMP in that miscibility is developed not at the leading edge (condensing region) or trailing edge(vaporizing region) of the displacement, but in between the condensing and vaporizing regions. Four primary methods have been used in recent years to determine MMPs for specific fluid displacements: slimtube experiments,10 compositional simulation,12 mixing-cell models, and analytical methods. Each of these methods has advantages and disadvantages. Slimtube experiments use real fluids but are expensive and time consuming to perform and can give misleading results depending on the level of physical dispersion present. Fine-grid compositional simulations and mixing-cell models can suffer from numerical-dispersion effects and are also time consuming to perform. Dispersion-free analytical methods are often very fast, but like simulation and mixing-cell models, they rely on an accurate fluid characterization by an EOS. A variety of correlations for the estimation of the MMP have been developed from regressions of slimtube data. Although less accurate, correlations are quick and easy to use and generally require only a few input parameters. Hence, they are very useful for fast screening of reservoirs for potential CO2flooding. They are also useful when detailed fluid characterizations are not available. One significant disadvantage of current MMP correlations is that the regressions use MMPs from slimtube data, which are themselves uncertain. Some MMP correlations require only the input of reservoir temperature and the API gravity of the reservoir fluid. Other, more-accurate correlations require reservoir temperature and the total C2-C6 content of the reservoir fluid. A few require detailed EOS characterizations. In nearly all of the correlations, the methane content of the oil is assumed to not affect the MMP significantly. Orr et al. show why this is true using analytical theory.
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Alam, R., J. York, M. Boyars, S. Stafford, J. A. Grant, J. Lee, P. Forsythe, T. Sim, and N. Ida. "Increased MCP-1, RANTES, and MIP-1alpha in bronchoalveolar lavage fluid of allergic asthmatic patients." American Journal of Respiratory and Critical Care Medicine 153, no. 4 (April 1996): 1398–404. http://dx.doi.org/10.1164/ajrccm.153.4.8616572.
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Cutuli, Salvatore L., Laurent Bitker, Eduardo A. Osawa, Zachary O’Brien, Emmanuel Canet, Fumitaka Yanase, Paolo Ancona, et al. "Haemodynamic effect of a 20% albumin fluid bolus in post-cardiac surgery patients." Critical Care and Resuscitation 22, no. 1 (March 2, 2020): 15–25. http://dx.doi.org/10.51893/2020.1.oa2.
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Objective: To study the cardiovascular effect over 30 minutes following the end of fluid bolus therapy (FBT) with 20% albumin in patients after cardiac surgery. Design: Prospective observational study. Setting: Intensive care unit of a tertiary university-affiliated hospital. Participants: Twenty post-cardiac surgery mechanically ventilated patients with a clinical decision to administer FBT. Intervention: FBT with a 100 mL bolus of 20% albumin. Main outcome measures: Cardiac index (CI) response was defined by a 15% increase, while mean arterial pressure (MAP) response was defined by a 10% increase. Results: The most common indication for FBT was hypotension (40%). Median duration of infusion was 7 minutes (interquartile range [IQR], 3–9 min). At the end of FBT, five patients (25%) showed a CI response, which increased to almost half in the following 30 minutes and dissipated in one patient. MAP response occurred in 11 patients (55%) and dissipated in five patients (45%) by a median of 6 minutes (IQR, 6–10 min). CI and MAP responses coexisted in four patients (20%). An intrabolus MAP response occurred in 17 patients (85%) but dissipated in 11 patients (65%) within a median of 7 minutes (IQR, 2–11 min). On regression analysis, faster fluid bolus administration predicted MAP increase at the end of the bolus. Conclusion: In post-cardiac surgery patients, CI response to 20% albumin FBT was not congruous with MAP response over 30 minutes. Although hypotension was the main indication for FBT and a MAP response occurred in most of patients, such response was maximal during the bolus, dissipated in a few minutes, and was dissociated from the CI response.
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Clair, Andrew, Matthew T. Kingery, Utkarsh Anil, Lena Kenny, and Eric Jason Strauss. "Synovial Fluid Biomarker Concentrations in Knees with Symptomatic Meniscus Injury Compared to Asymptomatic Contralateral Knees." Orthopaedic Journal of Sports Medicine 7, no. 7_suppl5 (July 2019): 2325967119S0035. http://dx.doi.org/10.1177/2325967119s00357.
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Objectives: Changes in the joint microenvironment following an intra-articular injury have been implicated in the pathogenesis of knee osteoarthritis. Few studies have evaluated alterations in the joint microenvironment in the setting of meniscus injury. The purpose of the current study was to determine the changes in synovial fluid biomarker concentrations caused by meniscus pathology by comparing samples from injured, symptomatic knees to samples from asymptomatic contralateral knees. Methods: Patients undergoing surgery for unilateral meniscus injury were prospectively enrolled in this institutional review board approved study from October 2011 to December 2016. A cohort was formed consisting of patients that had synovial fluid samples collected from both the injured and contralateral uninjured knee at the time of arthroscopic surgery. Patients with ligamentous injury of the knee were excluded from the current analysis. Synovial fluid samples were collected just prior to incision and the concentrations of 10 biomarkers of interest were determined using a multiplex magnetic bread immunoassay. Results: The current analysis included synovial fluid samples from 82 knees (41 operative and 41 contralateral knees) from 41 patients undergoing arthroscopic surgery to treat a symptomatic meniscus injury. The mean age of patients was 49.86 +/- 11.75 years. Based on linear mixed effects models, there were significantly greater concentrations of 4 of the 5 pro-inflammatory biomarkers in symptomatic knees compared to asymptomatic knees when controlling for the duration of symptoms, BMI, age, and the random effects of by-patient variability. Knees with symptomatic meniscus injuries had 126.8 times greater concentration of IL-6, 2.7 times greater concentration of MCP-1, 2.0 times greater concentration of MIP-1beta, and 5.4 times greater concentration of MMP-3 compared to the contralateral, asymptomatic knee (Table 1). When controlling for the chronicity of the injury, presence of synovitis, and age of the patient, knees with concomitant high-grade cartilage lesions (ICRS 3 or 4) were associated with 2.1 times greater concentration of MCP-1, 1.9 times greater concentration of MIP-1beta, and 3.4 times greater concentration of VEGF compared to knees with concomitant low-grade cartilage lesions (ICRS 1 or 2). When controlling for the other variables, the presence of synovitis was associated with an 89.5% lower concentration of TIMP-1 compared to operative knees without synovitis. The age of the patient was found to affect the concentrations of IL-6, MCP-1, and VEGF. For all knees included in the study, each 1 year increase in age was associated with a 6% increase in IL-6, 3% increase in MCP-1, and 4% increase in VEGF (Figure 1). Conclusion: This study is the first that examines the synovial fluid biomarker concentrations in the setting of a symptomatic isolated meniscus injury. We demonstrated that 4 of the 5 proinflammatory biomarkers that were tested are found in greater concentration in the symptomatic knee. Furthermore, we described the effects of associated cartilage damage, synovitis, and patient age on biomarker concentrations. Understanding the implication of these alterations in the intra-articular microenvironment in the setting of meniscal pathology may hold the key to identifying treatment targets in an effort to prevent the onset of post-meniscectomy osteoarthritis. [Table: see text]
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Lonappan, Anil, Vinu Thomas, G. Bindu, C. Rajasekaran, and K. T. Mathew. "Synovial fluid at microwave frequencies." Microwave and Optical Technology Letters 49, no. 7 (2007): 1700–1702. http://dx.doi.org/10.1002/mop.22552.
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