Dissertations / Theses on the topic 'Monoclonial antibodies'
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Chen, Desheng, and chen desheng@deakin edu au. "Development of monoclonal antibodies against Vibrio pathogens." Deakin University. Department of Biological Science, 1991. http://tux.lib.deakin.edu.au./adt-VDU/public/adt-VDU20080626.140825.
Full textAustin, Eric B. "Human monoclonal antibodies." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.276187.
Full textPlumpton, Christopher. "Monoclonal antibodies against phytochrome." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358677.
Full textRibeiro, Maricy Alves. ""Contribuição ao imunodiagnóstico da leptospirose humana: ênfase ao uso de anticorpos monoclonais"." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-15032004-161427/.
Full textThe best serological test for leptospirosis laboratory diagnosis remains the microscopic agglutination test (MAT). Because of the complexity of MAT, we have been developed some rapid screening tests for leptospiral antibodies detection in the acute phase of infection. In the decade of 80, a passive hemagglutination test employing polysaccharide fractions of leptospires was considered appropriate for early diagnosis, but its antigen preparation included common antigens recognized by antibodies from 4% of healthy individuals. A new ELISA (enzyme-linked immunosorbent assay) employing proteinase K resistant immunodominant antigens was developed and its potential diagnosis evaluated. This technique, the PK-ELISA, presented 89.9% sensitivity and 97.4% specificity, and satisfied the requeriments needed for serological screening tests of human leptospirosis. However, some of the reagents used in its antigen preparation are imported and very unstable. So, it was proposed, in a Cooperative Research Accordance between Instituto Adolfo Lutz and Laboratório Fleury, to try new approaches with monoclonal antibodies. Two hibridomas secreting specific monoclonal antibodies (MAb) were selected: one, against an epitope detected in 16 of 23 members of the genus Leptospira (clone A12P4) and the other, specific to the icterohaemorragiae serogroup (clone H7P1). The MAb A12P4, a G2 (IgG2B) immunoglobulin, reacted with an epitope present in the 16-18 kDa components of icterohaemorragiae serogroup and with the 75-84 kDa components of serovars copenhageni and canicola, after whole-cell lysates of the leptospires were separated by sodium dodecyl sulfate- polyacrylamide gel electrophoresis. The MAb H7P1, which is an IgG, reacted with an epitope common to several fractions of molecular weight above 21 kDa of strain RGA and with the 21-22 kDa and the 75-82 kDa components of strain M-20. Both monoclonal antibodies were employed in enzyme immunoassays for detecting specific antibodies in serum samples serially colleted from 52 patients with leptospirosis, and from the control group, which consisted of sera from 57 patients with other diseases included in the differential diagnosis, and from 68 healthy individuals. These tests, however, were not satisfactory. A new ELISA was developed in the present study employing an antigen suspension AgMc, purified by affinity chromatography with CNBr-activated Sepharose 4B coupled to the monoclonal antibodies described above. The results obtained with this test were compared to the MAT and to the classical IgM ELISA (ELISA c). The new method, AgMc ELISA, presented serological indices, relatively to reference test MAT, of 80.70 % and 83.33 % of sensitivity and specificity, respectively; positive and negative predictive values of 69.70 % and 90.10 %, respectively, and general agreement index of 82.49 %. So, this test was not considered a promising approach to rapid diagnosis of human leptospirosis. Moreover, the proportion of patients diagnosed as having leptospirosis by the AgMc ELISA and the MAT differ significantly. The possible explanations for the results obtained are discussed.
Qin, Shi-Xin. "Transplantation tolerance with monoclonal antibodies." Thesis, University of Cambridge, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305697.
Full textHeron, Andrew David. "The stability of monoclonal antibodies." Thesis, University of Glasgow, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.252169.
Full textIsaacs, John Dudley. "Improving serotherapy with monoclonal antibodies." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386115.
Full textBenjamin, Richard John. "Tolerance induction with monoclonal antibodies." Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.253988.
Full textPaudel, Subhash. "Shear thinning in monoclonal antibodies." Thesis, Kansas State University, 2016. http://hdl.handle.net/2097/32833.
Full textDepartment of Physics
Jeremy D. Schmit
Antibodies are large Y-shaped proteins which are used by immune system to identify and neutralize pathogens. Monoclonal antibody therapy is used to treat different patient conditions. There are problems associated with the manufacturability and deliverability of mAb solutions due to the viscous nature of the protein. The viscosity of antibody solutions increases with the increase in concentration and decreases with applied shear. We want to know why these behaviours are seen and to address this problem we have developed a theory describing the rapid viscosity increase with increasing concentration. We use the polymer theory to explain this behaviour. Here antibodies are treated as polymers. The length of the polymer depend on the aggregation. The reptation time increases approximately as the cubic power of size of aggregate (N³ ). We see the shear thinning behaviour is dependent on the Ab-Ab binding energy and find the relationship between the size of the aggregate and the binding energy. We find aggregate size and morphology using several models for Ab-Ab interaction sites. We use the head to head binding (fAb-fAb binding) model to describe aggregation state in our viscosity theory. The size of the aggregate and hence the reptation time is captured by the binding energy. When the binding energy increases the zero shear viscosity increases and the reptation time decreases. Likewise when the binding energy decreases the zero shear viscosity decreases and the reptation time increases. We have yet to find the correct exponents for the shear thinning behaviour of different mAbs which would be our future work.
Ueda, Yasuji. "MONOCLONAL ANTIBODIES TO CHICK CRYSTALLINS." 京都大学 (Kyoto University), 1989. http://hdl.handle.net/2433/86412.
Full textOhlin, Mats. "Human monoclonal antibody technology a tool to investigate human antibody repertoires /." Lund : Dept. of Immunotechnology, Lund University, 1992. http://catalog.hathitrust.org/api/volumes/oclc/39693827.html.
Full textYeung, Douglas Edward. "Characterization of six monoclonal antibodies against the Minute Virus of Mice NS-1 protein, and the use of one in the immunoaffinity purification of NS-1 expressed in insect cells." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/29405.
Full textMedicine, Faculty of
Biochemistry and Molecular Biology, Department of
Graduate
Mirza, Myriam. "Characterization of new CFTR monoclonal antibodies." Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=66882.
Full textA l'heure actuelle les anticorps dirigés contre la protéine CFTR ne sont pas suffisamment sensibles pour détecter cette protéine de facon endogène rendant ainsi l'étude de cette protéine difficile dans les tissus. Notre laboratoire a fabriqué deux anticorps monoclonaux , nommés 22E8 et 23C5, dirigés contre le domaine R de la protéine CFTR. L'abilité de ces anticorps à détecter l'expression de CFTR que ce soit de façon endogène ou lorsque la protéine est surexprimée a été testée à l'aide des techniques d'immunoblotting, d'immunoprécipitation et d'immunofluorescence. Afin de verifier leur sensibilité et leur capacité à détecter la protéine CFTR, ces anticorps ont été comparés aux anticorps M3A7 et 24-1 qui sont disponibles dans le commerce et connus pour détecter de facon optimale la protéine CFTR. Les resultats obtenus dans les lignées cellulaires à l'aide de la technique d'immunoblotting ont permis de montrer que les anticorps 23C5 et 22E8 sont plus sensibles que les anticorps commerciaux, de plus ils sont capables de détecter à la fois les protéines endogènes et sur-exprimées. Bien que l'anticorps 23C5 soit capable d'immunoprécipiter la protéine CFTR, aucun des deux anticorps n'a permis la detection de la protéine CFTR par immunoblotting dans les cellules de culture primaire. De plus, ces anticorps n'ont pas permis la detection de la protéine CFTR par immunofluorescence. Ainsi l'utilisation de ces anticorps nous donnera l'opportunité d'étudier la protéine CFTR dans les cellules l'exprimant de facon endogène afin de mieux comprendre sa regulation et son traffic.
Giorno, Caterina [Verfasser]. "Glycoengineering of Monoclonal Antibodies / Caterina Giorno." Konstanz : Bibliothek der Universität Konstanz, 2010. http://d-nb.info/1020366117/34.
Full textNoble, Philip W. "Characterisation of anti-glycan monoclonal antibodies." Thesis, University of Nottingham, 2011. http://eprints.nottingham.ac.uk/12071/.
Full textHoldsworth, Mary Louise. "Characterisation of phytochrome using monoclonal antibodies." Thesis, University of Leicester, 1987. http://hdl.handle.net/2381/35466.
Full textThanh, Le Thiet. "Exon-specific monoclonal antibodies against dystrophin." Thesis, University of Salford, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261661.
Full textWatson, Nigel. "Monoclonal antibodies to human immunoglobulin allotypes." Thesis, University of Strathclyde, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304897.
Full textOrtlepp, Susan. "Leucocyte integrin activation by monoclonal antibodies." Thesis, Open University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.359976.
Full textAlexandrovich, Susan K. "Characterization of monoclonal antibodies against digoxin /." Online version of thesis, 1987. http://hdl.handle.net/1850/10681.
Full textMachado, Marta Santos Serafim. "Estudo da imunogenicidade de antígenos de Neisseria lactamica: utilização de anticorpos monoclonais." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-14012009-102548/.
Full textImmunological and epidemiological evidences suggest that the development of natural immunity to meningogoccal disease may be associated with crossreactive antigens together with Neisseria meningitidis and other commensal bacteria, like Neisseria lactamica. The present study aimed to investigate the immunogenicity of antigens of outer membrane vesicles (OMV) of N. lactamica with or without the presence of Bordetella pertussis (BP) used as an adjuvant. Groups of neonate BALB/c mice were immunized intranasally antigens of N. lactamica. The results of our studies showed the predominance of high titers of antibodies of IgG and IgM isotipes with high and intermediate avidity after intranasal immunization with N. lactamica. Immunoblot analysis of serum showed cross-reactivity proteins between the species of the gender Neisseria and the monoclonal antibodies used in this study. These results suggest that antigens of N. lactamica and N. meningitidis in common may be important in natural immunity against meningogoccal disease and in the development of vaccine.
Telles, Andréia Elisa Rodrigues. "Clonagem e expressão de fragmentos de anticorpo de cadeia única (scFv) anti-LDL eletronegativa em Pichia pastoris." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-29092017-110802/.
Full textOxidative modification of low-density lipoproteins (LDL) is an essential step in atherogenesis, generating electronegative LDL [LDL(-)], which has chemotactic cytotoxic, immunogenic and proinflammatory properties. The aim of this study was the generation of anti-LDL(-) mAbs, the cloning of the genes that code for their variable domains and their expression as single-chain Fv (scFv). LDL(-) was isolated from human blood plasma and used as an antigen for immunization of Balb/c mice. Upon screening, two different mAbs were selected based on their ability to recognize LDL(-) and not native LDL: 1A3H2 (1A3) e 2C705F10 (2C7). The cDNAs that code for VH and VL were obtained by RT-PCR using specific immunoglobulin primer libraries wich recognize all VH and VL murine families. The VH and VL genes were cloned in pGEM-T Easy (Promega®) and sequenced. The anti-LDL(-) 1A3 uses a VH segment from J558.84 and a JH2 segment, while VL uses a 8.24/Jk5 segments. The anti-LDL(-) 2C7 uses a VH segment from Vmu 3.2 (J558) and a JH4 segment, while VL uses a 8.24/Jk5 segments. Oligonucleotides were synthetized and those gene segments were cloned in pPIGLE a Pichia pastoris immunoglobulin expression vector. We obtained three scFv constructions: scFV 1A3, scFv 2C7 and a husk hybrid, harboring 1A3 VH and 2C7 VL. Among those, we expressed the scFv anti¬-LDL(-) 2C7 that are able to recognize the antigen. The recombinant protein has a great potential for clinicai diagnostic applications, including in vitro immunoassays and as imaging reagents.
Scuro, Loren Semionatto. "Obtenção e estudo das propriedades de hibridomas produtores de anticorpos monoclonais anti-IL6 humana." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317407.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: O objetivo do presente trabalho foi a obtenção e caracterização de anticorpos monoclonais contra a IL6 humana recombinante (hr) para serem empregados em ensaios imunoenzimáticos do tipo ELISA (Enzyme linked immunosorbent assay) de detecção da IL6 humana nativa, presente em fluídos biológicos de pacientes portadores de quadros onde os níveis de IL6 encontram-se elevados, ou então produzida por monócitos humanos e murinos ativados in vitro. Dois grupos de hibridomas (1A6 e 3B1) secretores de anticorpos monoclonais anti-IL6 foram obtidos pela fusão de células de mieloma da linhagem SP2 Ag14/0 com esplenócitos de camundongos BALB/c, previamente imunizados com a IL6 humana recombinante. Esses hibridomas foram selecionados com base em sua reatividade com a hrIL6, através de ensaios do tipo ELISA indireto. As imunoglobulinas (Igs) monoclonais produzidas pelos hibridomas dos dois grupos são do isotipo IgG1 Kappa e foram purificadas de líquidos ascíticos e dos sobrenadantes de cultura por cromatografia de afinidade. As proteínas purificadas foram conjugadas com biotina para uso em ensaios de ELISA de captura da hrIL6, de modo a se identificar um ou mais pares de anticorpos adequados a esse tipo de teste, bem como para definir a sensibilidade de detecção da citocina. O par de anticorpos monoclonais 3B1E4 x 1A6F10-biotinilado se mostrou mais promissor nos ensaios de ELISA, detectando a citocina recombinante entre 8 e 512 ng/mL, liberando densidades óticas mais elevadas. Todos os anticorpos monoclonais (AcMos) anti IL6 estudados foram capazes de neutralizar a atividade biológica da citocina em ensaios empregando o hibridoma B13.9, uma célula dependente de IL-6 para seu crescimento. Finalmente, o par de hibridomas anti IL6 3B1E4 e 1A6F10 foi estudado quanto às suas principais características de cultivo: crescimento, produção in vitro dos anticorpos, consumo de glicose e produção de lactato e amônia. O seqüênciamento da porção N-terminal do par 3B1E4 e 1A6F10 revelou que as cadeias leves dos dois anticorpos apresentam seqüência idêntica de aminoácidos. Porém, a análise dos dez resíduos de aminoácidos presentes na região variável das cadeias pesadas resultou em seqüências completamente distintas nos dois monoclonais, sendo um forte indício de diferença nos sítios de ligação ao antígeno dos anticorpos estudados
Abstract: In the present study we have developed monoclonal antibodies against human recombinant (hr) IL6, for the use in ELISA assays to detect the human native IL6 present in biological fluid of patients or in supernatant of activated human and rodent monocytes. Two families of hibridomas (1A6 and 3B1) secreting MAb against-IL6 were obtained from the fusion of mieloma cells from SP2 Ag14/0 lineage with spleen cells from BALB/c mice, previously immunized with human recombinant IL6. Those hibridomas were selected on the basis of their reactivity with the hrIL6, through an ELISA indirect assay. The monoclonal immunoglobulins (Igs) produced by these hibridomas are from IgG1 Kappa isotype and were purified from ascitic fluid and culture supernatant by affinity chromatography. The purified proteins from the ascitic fluid were conjugated with biotin for the use in ELISA hrIL6 capture assay, to identify one or more pairs of antibodies appropriated to this kind of test, as well to define the sensibility of cytokine detection. The pair of MAbs 3B1E4 x 1A6F10-biotinilates was shown promising in ELISA assays, detecting the recombinant cytokine between 8 and 512 ng/mL, liberating elevated optical densities. All of the a-IL6 MAbs studied were capable to neutralize the biological activity of the cytokine in attempt employing the hibridoma B13.9 IL-6 dependent. Finally, the pair of hibridomas a-IL6 3B1E4 and 1A6F10 was studied as regards his main cultivation characteristics: growth, MAb production in vitro, lactate and ammonia production and glucose consumption. The N-Terminal portion sequence of 3B1E4 and 1A6F10 revealed that both light-chains present identical amino acid sequence. However, the analysis of the ten amino acid residues present in variable region of heavy-chains resulted in completely distinct sequences in both antibodies, being a strong indication of difference in their ability to recognize the antigen
Mestrado
Imunologia
Mestre em Genética e Biologia Molecular
Chen, Jianqing. "Radiolabeling and biotinylation of internalizing monoclonal antibody chimeric BR96 potential use for extracorporeal immunoadsorption with enhanced tumor radioactivity retention of iodine, indium and rhenium /." Lund : Lund University, the Jubileum Institute, Dept. of Radiation Physics, 1997. http://catalog.hathitrust.org/api/volumes/oclc/39725797.html.
Full textEvans, Rachael Yvonne. "The production of anti-idiotopic antibodies to monoclonal anti-RhD antibodies." Thesis, Lancaster University, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274194.
Full textMarques, Carlos Humberto. "Aspectos fundamentais à implantação da tecnologia de produção de anticorpos monoclonais humanizados com potencial aplicação terapêutica." Instituto de Tecnologia em Imunobiológicos, 2005. https://www.arca.fiocruz.br/handle/icict/5781.
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Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Os anticorpos monoclonais possuem diversasaplicações em transplantes, na composição de conjuntosde reativos para diagnóstico, grande variedade de doenças auto-imunes e, principalmente, na terapia do câncer. A tecnologia de produção de anticorpos monoclonais recombinantes revolucionou a geração de imunoglobulinas, possibilitando a obtenção de anticorpos humanizados dirigidos a uma grande variedade de antígenos específicos. A baixa seletividade das metodologias atuais para diagnóstico e terapia de neoplasiasconstitui um dos principais empecilhospara a prática oncológica. Nesse particular, a utilização de imunoglobulinas submetidas à engenharia genética já é uma realidade e significa um avanço estratégico, abrangendo cerca de 25% do mercado biofarmacêutico global de proteínas terapêuticas. Este trabalho aponta os aspectos fundamentais à concretização da metodologia de humanização de anticorpos por transplante das regiões determinantes de complementaridade – CDR, com ênfase em uma proposta de produção do anticorpo anti – CD20 contrao Linfoma Não-Hodgkin. A introdução do Instituto de Tecnologia emImunobiológicos - Bio-Manguinhos nesse promissor e importante mercado de biofármacos através da implantação da metodologia de humanização do anticorpo monoclonal murino anti-CD20 é objeto desta dissertação. Viabilizar sua produção torna-se extremamente importante, tanto para a identificação precisa e precoce da enfermidade, quanto para atender um segmento do mercado brasileiro ainda desprovido de tratamento abrangente e eficaz. A apresentação do estudo dos anticorpos, sua estrutura e características, o estudo dosdiferentes sistemas de expressão, cultivo, purificação,bem como a proposta de reestruturação e redimensionamento do Laboratório de Tecnologia de Anticorpos Monoclonais, parcerias, colaborações, recursos humanos necessários e aspectos de mercado, são aqui considerados.
Monoclonal antibodies (Mabs) have several applications in transplants, reagents for diagnosis, a great variety ofauto-immune diseases and mainly, in cancer therapy. Mabs production employing recombinant echnologymade a revolution in immunoglobulinsgeneration, enabling the production of humanized antibodiesthat recognize specific antigens. The low selectivity of the current techniquesfor neoplasm diagnosis and therapy is one of the major impediments for oncology practice. In this regard, the use of eneticallyengineered immunoglobulins has become a reality and meansa strategic development comprising around 25% of the global biopharmaceutical market. This work shows the most important aspects in Mabs humanization through complementary determining regions (CDR) graft methodology, emphasizing a proposal ofanti-CD20 Mab production against non-Hodgkin lymphoma. Introducing the Instituto de Tecnologia emImunobiológicos – Bio-Manguinhos in this important and promising biopharmaceutical market through the establishment of humanization methodology is the main object of this dissertation. Making humanized Mabs production feasible is veryimportant not only for the earlyand precise identification of illnesses, but also to meet a demand of the Brazilian market that still lacks comprehensive and efficient treatment. The study of Mabs, their structureand properties, expression systems, cultivation, purification, new dimensions and structure of the laboratory, partnerships, cooperation,human resources and market analysis are considered herein.
Storey, E. "Monoclonal antibodies to merozoites of Plasmodium falciparum." Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.371577.
Full textBanbury, David N. "New monoclonal antibodies to visualise vesicular compartments." Thesis, University of Oxford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.259782.
Full textKlutz, Stephan [Verfasser]. "Continuous processing of monoclonal antibodies / Stephan Klutz." München : Verlag Dr. Hut, 2016. http://d-nb.info/1115549731/34.
Full textHoney, C. R. "Immunosuppression with monoclonal antibodies in neural transplantation." Thesis, University of Oxford, 1990. http://ora.ox.ac.uk/objects/uuid:ea39dc7a-4ada-4c21-8cef-4649cb322646.
Full textHuang, Ling. "Investigation of soya globulins using monoclonal antibodies." Thesis, University of East Anglia, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302022.
Full textAnderson, J. "Investigations on bluetongue virus using monoclonal antibodies." Thesis, Open University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.374892.
Full textBell, Ian Martin. "Monoclonal antibodies as catalysts for cationic cyclisations." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387041.
Full textJones, Christine Ann. "Monoclonal antibodies in the study of neuropeptides." Thesis, Imperial College London, 1991. http://hdl.handle.net/10044/1/46848.
Full textCoswig, Lia Treptow. "Metapneumovirus aviario : suscetibilidade em diferentes sistemas celulares e produção de anticorpos monoclonais." [s.n.], 2008. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316628.
Full textTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: o Metapneumovírus Aviário (AMPV), também denominado vírus da rinotraqueíte dos perus (TRT), é um vírus que acomete e causa infecção no trato aéreo superior das galinhas e perus. Além da infecção respiratória, em poedeiras e matrizes está associado com uma queda significativa na produção de ovos. Em galinhas o vírus está relacionado com a Síndrome da Cabeça Inchada (SCI), uma enfermidade multifatorial, e por este motivo é importante o diagnóstico diferencial. Testes realizados com anticorpos monoclonais (Mabs) e técnicas moleculares são capazes de detectar diferenças entre os subtipos do vírus. Os métodos de diagnóstico incluem isolamento ou detecção da partícula viral ou testes sorológicos. O isolamento das amostras virais SHS-BR-121 (subtipo A) e STG-SHS-1439 (subtipo B) foi realizado em cultura de anel de traquéia, em fibroblasto de embrião de galinha (FEG) e em células chicken embryo related (CER). A comparação das médias dos títulos obtidos para as duas amostras virais, em célula CER, apresentou diferença estatisticamente significativa (P=0,014) com p< 0,05. Neste projeto foi avaliada a suscetibilidades de seis sistemas celulares (CER, Vero, BHK-21, HEp-2, MDBK e ED) para a multiplicação das duas amostras virais (subtipos A e B). Destes sistemas as células CER, Vero e BHK-21 demonstraram ser apropriadas para a replicação vira!. Os títulos nestas células variaram de 105.5 a 107,o/mL DICC50 , para o vírus SHS-BR-121, e 105,5 a 106.0/mL DICC50 para o vírus STG-SHS-1439. As diferenças entre as médias dos títulos nos diferentes sistemas celulares foi estatisticamente significativa para a amostra SHS-BR-121 inoculada em CER em relação as células Vero e BHK-21 (P= 0,01 e P=0,004, respectivamente) com p< 0,05. Para a amostra STG-SHS-1439 não houve diferença estatística significativa, com p<0,05. A curva da cinética viral foi realizada para as duas amostras virais, em três sistemas celulares, CER, Vero e BHK-21, demonstrando estas diferenças. Foram produzidos anticorpos monoclonais contra o AMPV isolado no Brasil, sendo obtidos cinco anticorpos monoclonais para o antígeno viral através da fusão celular que apresentaram os isotipos IgG1, IgG2a e IgG2b quando da isotipagem. Dos cinco anticorpos monoclonais, três possuíam atividade neutralizante e quatro deles inibiram a fusão invitro. No teste de soroneutralização cruzada foram utilizadas três amostras virais para a análise, sendo elas SHS-Br-121, STG 854/88 e TRT-SHS-1439. Todos os anticorpos monoclonais apresentaram resultado positivo em relação à amostra homóloga, sendo que três apresentaram resultados positivos também para as amostras heterólogas. Os resultados confirmam que os dois anticorpos monoclonais descritos podem ser utilizados com importante ferramenta nos estudos epizootiológicos e para o diagnóstico específico dos subtipos na infecção pelo Metapneumovírus Aviário
Abstract: Avian Metapneumovirus (AM PV) , also denominated virus of the rhinotracheitis of the turkeys, it is a virus that attacks and it causes infection in the upper respirator) tract of chickens and turkeys. Besides the respiratory infection, in breeders and layers is associated with a significant fall in the production of eggs. In chickens the virus is related with the Swollen Head Syndrome (SHS), an illness multifactorial and for this reason it is very important the differential diagnosis. Tests accomplished with monoclonal antibodies (Mabs) and molecular techniques are capable to detect differences among the subtypes of the virus. Methods for the diagnosis of AMPV infections include detection or isolation of the virus itself, demonstration of a specific antibody response to the virus. For the primary isolation of the two samples of AMPV SHS-BR-121 (subtype A) and STG-SHS-1439 (subtype B) was it accomplished ir chicken embryo tracheal" organ culture (TOC), in chicken embryo fibroblast cell culture (CEF) and was it accomplished in cell line chicken embryo related (CER). Done the comparison of the averages of the titers obtain for the two strains, in cellline CER, did present statistically significant difference (P=0,014) with p< 0,05. The growth of SHS121-BR and STG-SHS-1439 was evaluated in six different celllines (CER, Vero, BHK21, HEp-2, MDBK and ED). CER, Vero and BHK-21 showed to be the most appropriate for virus multiplication. The titers in these cells varied from 105.5 to 107,o/mL !CID50, for the virus SHS-BR-121, and 105,5 to 106,o/mL TCID50 for the virus STG-SHS71439. The differences among the averages of the titers in the different cell lines was statistically significant differences (P=0,01) with p<0,05 for the strain SHS-BR-121 in CER cellline. One-step growth curves of the strains SHS-BR-121 and STG-SHS-1439 in CER, Vero and BHK-21 showed that there was not statistically significant difference in the infectious virus titers from 0 to 60 hours after infection. Five monoclonal antibodies were obtained for the viral antigen through the cell fusion that showed the isotypes IgG1, IgG2a and IgG2b, when of the characterization of Mabs. Three of them showed neutralizing activity and four inhibited the fusion in vitro. These MAbs were used to investigate antigenic relationship among three strains (SHS-BR-121, STG 854/8o/and TRT1439/91) of a MPV subtypes A and B using cross-neutralization test. When the five hybridomas were analyzed showed result positive for the homologus virus. In relation t4 two samples of heterologus AMPV three Mabs were positive to heterologus AMPV. Th4 results confirm that the two monoclonal antibodies described can be used as a valuable tool in the epizootiological and serological studies, and also for the specific diagnosis o the subtypes in the infection for Avian Metapneumovirus
Doutorado
Microbiologia
Doutor em Genetica e Biologia Molecular
Hills, Anna E. "Control of monoclonal antibody N-glycosylation." Thesis, University of Kent, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.344101.
Full textEastwood, David Geoffrey Douglas. "Immunotoxicology of the therapeutic monoclonal antibody TGN1412." Thesis, St George's, University of London, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.676898.
Full textWatkins, I. D. "Monoclonal antibodies to Legionella pneumophila and related organisms." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.354879.
Full textTellides, George. "Immunosupression monoclonal antibodies to rat lymphocyte activation antigens." Thesis, University of Oxford, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236271.
Full textChia, T. W. H. "The use of monoclonal antibodies for glycoside hydrolysis." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.597597.
Full textMason, Caroline Margaret. "Characterisation of intestinal M cells using monoclonal antibodies." Thesis, University of Newcastle Upon Tyne, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260952.
Full textPerera, W. Shermal. "Binding and molecular characterisation of monoclonal RhD antibodies." Thesis, University of Aberdeen, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342706.
Full textDuguid, I. G. M. "Prevention of corneal graft rejection with monoclonal antibodies." Thesis, University of Aberdeen, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387460.
Full textDe, Silva M. G. "Human monoclonal antibodies in the study of diabetes." Thesis, University of Surrey, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233146.
Full textTsim, Karl Wah-Keung. "Chick acetylcholinesterase : purification, molecular properties and monoclonal antibodies." Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236054.
Full textGilliland, Lisa Kim. "The development of bispecific monoclonal antibodies for therapy." Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278214.
Full textBuffetto, Fanny. "Generation of new monoclonal antibodies to rhamnogalacturonan fragments." Nantes, 2014. https://archive.bu.univ-nantes.fr/pollux/show/show?id=85b86558-be8b-4c38-ae82-702e14160fa0.
Full textPectins are widely present in middle lamellae and primary cell walls of dicots. They are composed of different covalently linked structural domains. Rhamnogalacturonan II and rhamnogalacturonan I are the most complex pectic domains. Rhamnogalacturonan II is composed of a homogalacturonan backbone, which carries five different side chains containing rare sugars. Rhamnogalacturonan I has a heterobackbone of rhamnoses and galacturonic acids. This domain contains linear or branched arabinose ‐ and galactose ‐ rich chains. To localize these structures in planta, immunofluorescence microscopy is a sensitive technique. Immunolabelling is performed with antibodies, recognizing specific structures. Some antibodies, which label pectins are already available. However, the production of new probes is required. To generate new antibodies, oligosaccharides from rhamnogalacturonan II and rhamnogalacturonan I were purified. Structural analyses revealed the high diversity of motifs present in rhamnogalacturonan I. Immunizations carried out with the different oligosaccharides have enabled the generation of a new monoclonal antibody binding to rhamnogalacturonan I side chains. This antibody recognizes a molecular motif present in galactan rich sources, which has been poorly described in literature. This structure was localized in planta, in potato tuber and in roots of seedling Arabidopsis. However, function of this structure remains to be determined
Bell, Graham Thomas. "Studies on the production of human monoclonal antibodies." Thesis, University of Edinburgh, 1988. http://hdl.handle.net/1842/19241.
Full textSun, Ping. "Studies of sperm antigens using specific monoclonal antibodies." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/28405.
Full textMedicine, Faculty of
Obstetrics and Gynaecology, Department of
Graduate
Antonialli, Renan. "Efeito de ligantes de receptores semelhantes a Toll na resposta imune induzidas por antígenos direcionados ao DEC205 e DCIR2." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/42/42135/tde-03062014-160754/.
Full textDendritic cells (DCs) express receptors that recognize pathogen-associated molecular patterns (PAMPs), and are able to capture, process and present antigens to lymphocytes. In recent years, a immunization strategy that directs antigens to DCs in vivo has been developing. This consists in the use of monoclonal antibodies fused with the antigen of interest. However, effective immune activation occurs mainly when the chimeric antibodies anti-DEC or anti-DCIR2 are administered in the presence of a DC maturation stimulus. We immunized wild type and knockout mice for TLR3, 5 and 9 with adjuvants (poly I:C, CpG ODN 1826 and flagellin from S. typhimurium) and chimeric anti-DEC205, anti-DCIR2 or isotype control fused to the Plasmodium vivax MSP119 protein. After the administration of two doses, we evaluated the humoral and cellular immune responses in the different groups. Our results indicate that, irrespectively of the adjuvant, the majority of parameters analyzed points out to the superiority of antigen targeting to the DEC205 receptor.