Relevant bibliographies by topics / Monoclonal antibodies

Academic literature on the topic 'Monoclonal antibodies'

Author: Grafiati
Published: 4 June 2021
Last updated: 8 June 2024

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Monoclonal antibodies.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Journal articles on the topic "Monoclonal antibodies":

1

Rieger, Paula Trahan. "Monoclonal Antibodies." American Journal of Nursing 87, no. 4 (April 1987): 469. http://dx.doi.org/10.2307/3470440.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Kosmas, C., H. Kalofonos, and A. A. Epenetos. "Monoclonal Antibodies." Drugs 38, no. 5 (November 1989): 645–57. http://dx.doi.org/10.2165/00003495-198938050-00001.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

&NA;. "Monoclonal antibodies." Reactions Weekly &NA;, no. 1293 (March 2010): 36. http://dx.doi.org/10.2165/00128415-201012930-00100.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

&NA;. "Monoclonal Antibodies." Journal of Pediatric Hematology/Oncology 25, no. 4 (April 2003): S5—S6. http://dx.doi.org/10.1097/00043426-200304000-00025.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

&NA;. "Monoclonal Antibodies." Journal of Pediatric Hematology/Oncology 25, no. 4 (April 2003): S17—S18. http://dx.doi.org/10.1097/00043426-200304000-00036.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Anderson, Philip O. "Monoclonal Antibodies." Breastfeeding Medicine 11, no. 3 (April 2016): 100–101. http://dx.doi.org/10.1089/bfm.2016.0026.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Nowak, Thomas P. "Monoclonal Antibodies." American Journal of Clinical Oncology 10, no. 4 (August 1987): 278–80. http://dx.doi.org/10.1097/00000421-198708000-00002.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Ghobrial, Rafik M., Ronald W. Busuttil, and Jerzy W. Kupiec-Weglinski. "Monoclonal antibodies." Current Opinion in Organ Transplantation 2, no. 1 (October 1997): 82–88. http://dx.doi.org/10.1097/00075200-199710000-00015.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Rosen, Steven T., Elyse A. Lambiase, Yixing Ma, James A. Radosevich, and Alan L. Epstein. "Monoclonal antibodies." Postgraduate Medicine 77, no. 4 (March 1985): 129–34. http://dx.doi.org/10.1080/00325481.1985.11698922.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Rabin, Brace S. "Monoclonal antibodies." Postgraduate Medicine 79, no. 1 (January 1986): 293–303. http://dx.doi.org/10.1080/00325481.1986.11699254.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources
You might also be interested in the extended bibliographies on the topic 'Monoclonal antibodies' for particular source types:
Journal articles Dissertations / Theses Books

Dissertations / Theses on the topic "Monoclonal antibodies":

1

Austin, Eric B. "Human monoclonal antibodies." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.276187.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Plumpton, Christopher. "Monoclonal antibodies against phytochrome." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.358677.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Benjamin, Richard John. "Tolerance induction with monoclonal antibodies." Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.253988.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Qin, Shi-Xin. "Transplantation tolerance with monoclonal antibodies." Thesis, University of Cambridge, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305697.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Heron, Andrew David. "The stability of monoclonal antibodies." Thesis, University of Glasgow, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.252169.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Isaacs, John Dudley. "Improving serotherapy with monoclonal antibodies." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386115.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Paudel, Subhash. "Shear thinning in monoclonal antibodies." Thesis, Kansas State University, 2016. http://hdl.handle.net/2097/32833.

Full text
APA, Harvard, Vancouver, ISO, and other styles
Abstract:
Master of Science
Department of Physics
Jeremy D. Schmit
Antibodies are large Y-shaped proteins which are used by immune system to identify and neutralize pathogens. Monoclonal antibody therapy is used to treat different patient conditions. There are problems associated with the manufacturability and deliverability of mAb solutions due to the viscous nature of the protein. The viscosity of antibody solutions increases with the increase in concentration and decreases with applied shear. We want to know why these behaviours are seen and to address this problem we have developed a theory describing the rapid viscosity increase with increasing concentration. We use the polymer theory to explain this behaviour. Here antibodies are treated as polymers. The length of the polymer depend on the aggregation. The reptation time increases approximately as the cubic power of size of aggregate (N³ ). We see the shear thinning behaviour is dependent on the Ab-Ab binding energy and find the relationship between the size of the aggregate and the binding energy. We find aggregate size and morphology using several models for Ab-Ab interaction sites. We use the head to head binding (fAb-fAb binding) model to describe aggregation state in our viscosity theory. The size of the aggregate and hence the reptation time is captured by the binding energy. When the binding energy increases the zero shear viscosity increases and the reptation time decreases. Likewise when the binding energy decreases the zero shear viscosity decreases and the reptation time increases. We have yet to find the correct exponents for the shear thinning behaviour of different mAbs which would be our future work.
8

Ueda, Yasuji. "MONOCLONAL ANTIBODIES TO CHICK CRYSTALLINS." 京都大学 (Kyoto University), 1989. http://hdl.handle.net/2433/86412.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Pathan, N. "Catalytic monoclonal antibodies: a review." Thesis(M.Phil.), CSIR-National Chemical Laboratory, Pune, 1990. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/2017.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Alexandrovich, Susan K. "Characterization of monoclonal antibodies against digoxin /." Online version of thesis, 1987. http://hdl.handle.net/1850/10681.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "Monoclonal antibodies":

1

Ossipow, Vincent, and Nicolas Fischer, eds. Monoclonal Antibodies. Totowa, NJ: Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-992-5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Albitar, Maher, ed. Monoclonal Antibodies. Totowa, NJ: Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-323-3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Peters, Johann Hinrich, and Horst Baumgarten, eds. Monoclonal Antibodies. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Peter, Beverley, ed. Monoclonal antibodies. Edinburgh: Churchill Livingstone, 1986.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
5

JoAnn, Edwards-Moulds, and Masouredis Serafeim P, eds. Monoclonal antibodies. Arlington, Va: American Association of Blood Banks, 1989.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
6

L, Beverley Peter C., ed. Monoclonal antibodies. Edinburgh: Churchill Livingstone, 1986.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
7

P, Weizer William, Martineau William D, and Freedonia Group, eds. Monoclonal antibodies. Cleveland, Ohio: Freedonia Group, Inc., 1991.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
8

Borrebaeck, Carl A. K., and James W. Larrick, eds. Therapeutic Monoclonal Antibodies. London: Palgrave Macmillan UK, 1990. http://dx.doi.org/10.1007/978-1-349-11894-6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Steinitz, Michael, ed. Human Monoclonal Antibodies. Totowa, NJ: Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-586-6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

An, Zhiqiang, ed. Therapeutic Monoclonal Antibodies. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2009. http://dx.doi.org/10.1002/9780470485408.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "Monoclonal antibodies":

1

Peters, J. H., and D. Baron. "Introduction." In Monoclonal Antibodies, 1–17. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4_1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Peters, J. H., M. Schulze, M. Grol, S. Schiefer, H. Baumgarten, J. Endl, H. Xu, et al. "Demonstration of Monoclonal Antibodies." In Monoclonal Antibodies, 316–461. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4_10.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Baron, D. "Safety Precautions at Work." In Monoclonal Antibodies, 463–65. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4_11.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Peters, Johann Hinrich, and Horst Baumgarten. "Appendix." In Monoclonal Antibodies, 466–74. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4_12.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Wiggenhauser, A., J. H. Peters, and H. Baumgarten. "Preconditions for Hybridoma Technology." In Monoclonal Antibodies, 18–38. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4_2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Baumgarten, H., M. Schulze, J. H. Peters, and T. Hebell. "Immunization." In Monoclonal Antibodies, 39–70. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4_3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Wiggenhauser, A., J. H. Peters, H. Baumgarten, and A. Borgya. "Taking Blood and Isolating Cells." In Monoclonal Antibodies, 71–87. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4_4.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Peters, J. H., E. Debus, H. Baumgarten, R. Würzner, M. Schulze, and Helga Gerlach. "Cell Culture." In Monoclonal Antibodies, 88–136. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4_5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Baron, D., J. H. Peters, R. K. H. Gieseler, S. Lenzner, H. Baumgarten, R. Würzner, B. Goller, and Th Werfel. "Production of Hybridomas." In Monoclonal Antibodies, 137–222. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4_6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Baumgarten, H., R. Franze, J. H. Peters, A. Borgya, D. Baron, E. Debus, and M. Kubbies. "Mass Production of Monoclonal Antibodies." In Monoclonal Antibodies, 223–57. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4_7.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Monoclonal antibodies":

1

Kiefel, V., S. Santoso, and C. Mueller-Eckhardt. "ANALYSIS OF PLATELET REACTIVE ANTIBODIES USING MONOCLONAL ANTIBODIES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643929.

Full text
APA, Harvard, Vancouver, ISO, and other styles
Abstract:
The characterization of platelet reactive alloantibodies and autoantibodies is mandatory for the diagnosis of posttransfusion purpura, neonatal alloimmune thrombocytopenia, autoimmune thrombocytopenia and for the selection of platelet donors prior to platelet transfusions in immunized polytransfused patients. The platelet immunofluorescence test is suitable for the detection of platelet reactive antibodies. In many cases, however, mixtures containing different platelet reactive antibodies have to be dissected.In order to analyze these sera, we have developed a novel enzyme immunoassay based upon monoclonal antibody specific immobilization of platelet antigens (MAIPA). In brief, platelets are incubated simultaneously with the (human) serum to be investigated and a monoclonal (mouse) antibody directed against an epitope on the same platelet membrane glycoprotein (GP). Platelets are then washed and solubilized in TRIS buffered saline containing NP40. The lysed platelets are then pipetted into the wells of microtiter plates, coated with goat anti mouse IgG where mouse anti GP-complexes are immobilized. Human platelet reactive antibodies on the same GP are detected using enzyme labelled goat anti human IgG, IgM, or IgA, respectively. Using mab Gi5, mab FMC25, mab w6.32 directed against epitopes on the glycoprotein complex IIb/IIIa, glycoprotein Ib and HLA class I molecule, respectively, and a panel of typed platelet donors, even sera containing different platelet reactive antibodies are readily analyzed. Results of experiments with platelet specific alloantibodies (anti P1A1, anti P1A2 and anti Bak(a)), autoantibodies (against the GP Ilb/IIIa complex and GP Ib) and a drug dependent antibody show that this assay allows to discriminate all these different platelet reactive antibodies.
2

Sun, Yanjie, Zhuoyun Wan, and Zixiao Zhang. "Therapeutic Monoclonal Antibodies: Clinical Applications." In International Conference on Biotechnology and Biomedicine. SCITEPRESS - Science and Technology Publications, 2022. http://dx.doi.org/10.5220/0012020800003633.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Yang, Wu, Li-ming Wang, Zhao Wei, and Yuan Junlin. "Preliminary Production of Anti- Glufosinate Monoclonal Antibodies." In 2007 1st International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2007. http://dx.doi.org/10.1109/icbbe.2007.18.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Young, Colin R., Alice Lee, and Larry H. Stanker. "Detection of Campylobacter species using monoclonal antibodies." In Photonics East (ISAM, VVDC, IEMB), edited by Yud-Ren Chen. SPIE, 1999. http://dx.doi.org/10.1117/12.335779.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Ray, Jason C., Penelope Allen, Ann Bacsi, Julian Bosco, Luke Chen, Michael Eller, Lyndell Lim, et al. "076 Inflammatory complications of CGRP monoclonal antibodies." In ANZAN Annual Scientific Meeting 2021 Abstracts. BMJ Publishing Group Ltd, 2021. http://dx.doi.org/10.1136/bmjno-2021-anzan.76.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Metzelaar, M. J., H. K. Nieuwenhuis, and J. J. Sixma. "DETECTION OF ACTIVATED PLATELETS WITH MONOCLONAL ANTIBODIES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643829.

Full text
APA, Harvard, Vancouver, ISO, and other styles
Abstract:
Blood tests reflecting in-vivo activation of platelets are potentially useful in evaluating patients with thrombotic diseases. Recently monoclonal antibodies have been described that react preferentially with activated platelets. We prepared an IgG2b antibody, designated RUU-AP 2.28, that reacted with a 53.000 MW protein that is located in a special subclass of platelet granules in unstimulated platelets and that is exposed on the surface of activated platelets. Increased numbers of platelets that expressed the 2.28 antigen on their surface were observed in patients undergoing cardiopulmonary bypass and in patients with acute deep venous thrombosis. The percentage of RUU-AP 2.28 positive platelets in the circulation was 3,9 ± 2.7 (SD)% in the controls, (n = 20), 24.6 ± 13.5% in patients after cardiopulmonary surgery (n = 10) and 8.5% in patients with acute deep venous thrombosis (n = 2).In order to detect also earlier stages of platelet activation, such as secretion-independent phenomena, we produced new monoclonal antibodies by fusing spleen cells from Balb/c mice, immunized with thrombin stimulated, paraformaldehyde fixed platelets, with Ag 8653 myeloma cells. As a screening assay we used an ELISA with freshly fixed platelets or fixed thrombin-activated platelets. We detected six monoclonal antibodies (RUU-AP 1-6) specific for thrombin-activated platelets. The results of the ELISA were confirmed by flow cytofluorometry.None of the antibodies inhibited platelet aggregation induced by ADP, collagen or ristocetin. Ascites of IgGl antibody RUU-AP 3 reacted with normal thrombin-activated platelets but did not react with thrombin-activated platelets from a patient with Glanzmann’s disease. In addition antibody RUU-AP 3 reacted with normal platelets stimulated with 1 pM of ADP. These data suggest that antibody RUU-AP 3 detects a secretion-independent conformational change in the platelet membrane glycoprotein IIb-IIIa complex.
7

Brown, Michael C., Ross Chambers, Dale V. Onisk, Tony R. Joaquim, Lewis J. Stafford, Klaus Lindpaintner, Daniel Keter, and James W. Stave. "Abstract 4325: Monoclonal antibodies to transmembrane proteins." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-4325.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Hessing, Martin, Joost C. M. Meijers, Jan A. van Mourik, and Bonno N. Bouma. "MONOCLONAL ANTIBODIES TO HUMAN PROTEIN S AND C4b-BINDING PROTEIN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644291.

Full text
APA, Harvard, Vancouver, ISO, and other styles
Abstract:
Protein S (PS) circulates in plasma both free and in reversible association with the complement component C4b-binding protein (C4bp). Only free PS is functional as a cofactor for activated protein C (APC). Cleavage of PS by thrombin at a site near the r-carboxyglutamic acid domain is associated with a loss of cofactor activity. This may be a control mechanism for the anticoagulant activity of APC. These observations led us to investigate the role of C4bp and thrombin in the regulation of PS. Complex formation between purified PS and C4bp was studied in plasma and in a system with purified components. 125I-labeled PS was first incubated with either C4bp or citrated plasma and then subjected to polyacrylamide gelelectrophoresis in the absence of SDS. The formation of the C4bp-PS complex in plasma and in the purified system was demonstrated by autoradiography. Crossed immuno-electrophoresis using an antiserum against PS was performed in the presence of 8 mM EDTA. Human citrated plasma showed two precipitin peaks. Free PS migrated rapidly in the first dimension, whereas the C4bp-PS complex was just anodal to the application slot. The addition of C4bp to either plasma or purified PS resulted in the disappearance of the free PS peak and an increase of the slower migrating peak. The effect of purified C4bp on the PS-cofactor function of APC was studied in citrated plasma. The prolongation of the APTT induced by the addition of APC could be inhibited by the addition of increasing amounts of C4bp. Monoclonal antibodies to PS and C4bp were prepared and characterized. The monoclonal antibodies to either PS or C4bp did not block the complex formation between and PS, as was demonstrated by dot blotting of C4bp with 125I-PS and agarose gelelectrophoresis followed by Western blotting. Three out of 7 monoclonal antibodies to PS did not detect PS after thrombin cleavage on an immunoblot after non-reduced SDS polyacrylamide gelelectrophoresis. These 3 antibodies gave a significant shortening of the prolonged APTT induced by the addition of APC to normal plasma, indicating that these monoclonals inhibited the cofactor function of PS. The other 4 monoclonals to PS that did detect PS after thrombin cleavage on an immunoblot, gave only a minor inhibition of the PS cofactor function.
9

Chumak, N. S., and Y. I. Melnikova. "OBTAINING AND IMMUNOCHEMICAL TESTING OF APOFERRITIN." In SAKHAROV READINGS 2022: ENVIRONMENTAL PROBLEMS OF THE XXI CENTURY. International Sakharov Environmental Institute of Belarusian State University, 2022. http://dx.doi.org/10.46646/sakh-2022-1-289-293.

Full text
APA, Harvard, Vancouver, ISO, and other styles
Abstract:
The process of direct binding of monoclonal antibodies to apoferritin immobilized on polystyrene, as well as the method of competitive interaction of monoclonal antibodies with apoferritin in solution, have been experimentally studied. It was found that the immobilization of apoferritin on a polystyrene surface leads to a change in the epitope structure of this protein and to the elimination of reactive epitopes of binding of monoclonal antibodies. The soluble form of apoferritin effectively binds to monoclonal antibodies in a competitive assay, which confirms the conformational nature of the clusters of determinants on the surface of apoferritin.
10

Clemetson, K. J., R. Weber, and J. L. McGregor. "TOPOLOGY OF PLATELET GPIb INVESTIGATED BY LOCATION OF MONOCLONAL ANTIBODY EPITOPES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643625.

Full text
APA, Harvard, Vancouver, ISO, and other styles
Abstract:
A large number of monoclonal antibodies to platelet membrane glycoprotein lb (GPIb) have been described but for most of these the position of the epitope is not known. Since many of these influence platelet function, a better understanding of struc-ture-function relationships requires this knowledge. The position of the epitopes for the monoclonal antibodies API (Dr. T.J. Kunicki), AN51 and SZ-2 (Dr. C-G. Ruan), WM23 (Dr. M.C. Berndt) and PI were determined by analysis of proteolytic cleavage fragments of glycocalicin via affinity chromatography on the monoclonal antibodies coupled to Sepharose, elution with diethyl ami ne solution, separation on SDS-gel electrophoresis and detection by silver-staining. First, intact glycocalicin was examined and was found to bind to all monoclonals with the exception of PI. All monoclonals bound intact GPIb. WM23 bound a 70 kDa glycopeptide from the highly-glycosylated 90 kDa tryptic fragment of glycocalicin. API, AN51 and SZ-2 all bound to 45 kDa and 40 kDa, poorly glycosylated tryptic fragments. The 40 kDa fragment is derived from the 45 kDa fragment and has been shown to be the N-terminal region of GPIb. All these monoclonals have been shown to inhibit von Willebrand factor induced platelet agglutination. Platelets were treated with either elastase or calcium activated protease and monoclonal binding checked by immunofluorescence. The immunofluorescence with API, AN51 and SZ-2 was minimal compared to control platelets whereas that of PI remained as strong as the controls. This indicates that the epitope for PI lies on GPIb in a region other than glycocalicin and its absence from glycocalicin is not simply due to conformational changes in that fragment. Since PI inhibits platelet activation by thrombin and ADP it must act via conformational effects and not by blocking the thrombin receptor which lies on the 45 kDa region of glycocalicin. These results support a more complex role for GPIb in platelet activation.

Reports on the topic "Monoclonal antibodies":

1

Snyder, Christopher M., and Lawrence J. Wysocki. Dissecting Immunogenicity of Monoclonal Antibodies. Fort Belvoir, VA: Defense Technical Information Center, June 2002. http://dx.doi.org/10.21236/ada407659.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Snyder, Christopher M., and Lawrence J. Wysocki. Dissecting Immunogenicity of Monoclonal Antibodies. Fort Belvoir, VA: Defense Technical Information Center, June 2003. http://dx.doi.org/10.21236/ada417364.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Jaszczak, R. J. SPECT assay of radiolabeled monoclonal antibodies. Office of Scientific and Technical Information (OSTI), February 1992. http://dx.doi.org/10.2172/7197646.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Jaszczak, R. J. SPECT assay of radiolabeled monoclonal antibodies. Office of Scientific and Technical Information (OSTI), February 1992. http://dx.doi.org/10.2172/7288347.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Chia, John K. Polymyxin B(PMB)-Specific Monoclonal Antibodies. Fort Belvoir, VA: Defense Technical Information Center, January 1991. http://dx.doi.org/10.21236/ada231817.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Ivy, John M. Production of Anti-Ferret IgA Antibodies; and production of monoclonal antibodies. Fort Belvoir, VA: Defense Technical Information Center, April 1994. http://dx.doi.org/10.21236/ada279534.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Sato, J. D. Receptor Monoclonal Antibodies that Inhibit Tumor Angiogenesis. Fort Belvoir, VA: Defense Technical Information Center, August 2001. http://dx.doi.org/10.21236/ada398146.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Sato, J. D. Receptor Monoclonal Antibodies that Inhibit Tumor Angiogenesis. Fort Belvoir, VA: Defense Technical Information Center, August 1999. http://dx.doi.org/10.21236/ada383129.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Glassy, Mark C. Neutralizing Monoclonal Antibodies against Biological Toxins. Phase 1. Fort Belvoir, VA: Defense Technical Information Center, August 1993. http://dx.doi.org/10.21236/adb176298.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Jaszczak, Ronald, J. Final Progress Report: SPECT Assay of Radiolabeled Monoclonal Antibodies. Office of Scientific and Technical Information (OSTI), September 2004. http://dx.doi.org/10.2172/886018.

Full text
APA, Harvard, Vancouver, ISO, and other styles

To the bibliography