Relevant bibliographies by topics / Molecular virology

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Reports

Academic literature on the topic 'Molecular virology'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 August 2024

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Journal articles on the topic "Molecular virology"

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Doorbar, John. "Molecular virology." Trends in Microbiology 3, no. 2 (February 1995): 80. http://dx.doi.org/10.1016/s0966-842x(00)88883-6.

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Stephenson, Iain. "Influenza: Molecular Virology." Expert Review of Vaccines 9, no. 7 (July 2010): 719–20. http://dx.doi.org/10.1586/erv.10.71.

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Whitley, Richard. "Alphaherpesviruses: Molecular Virology." Antiviral Therapy 17, no. 2 (2011): 409. http://dx.doi.org/10.3851/imp1929.

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Bangham, C. R. M. "Practical Molecular Virology." Journal of Medical Genetics 30, no. 6 (June 1, 1993): 536. http://dx.doi.org/10.1136/jmg.30.6.536-a.

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Netesov, S. V., and N. A. Markovich. "Introduction to molecular virology." Russian Journal of Genetics: Applied Research 4, no. 4 (July 2014): 325–39. http://dx.doi.org/10.1134/s2079059714040078.

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Cane, P. "Molecular Virology, 2nd edn." Journal of Antimicrobial Chemotherapy 43, no. 1 (January 1, 1999): 168. http://dx.doi.org/10.1093/jac/43.1.168.

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Vernet, Guy. "Molecular diagnostics in virology." Journal of Clinical Virology 31, no. 4 (December 2004): 239–47. http://dx.doi.org/10.1016/j.jcv.2004.06.003.

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Lachman, Robin. "Molecular virology in brief." Molecular Medicine Today 5, no. 2 (February 1999): 55. http://dx.doi.org/10.1016/s1357-4310(98)01412-9.

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Elliott, Richard M. "Molecular virology made simple." Trends in Microbiology 2, no. 8 (August 1994): 300–301. http://dx.doi.org/10.1016/0966-842x(94)90011-6.

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Brierley, Ian. "Principles of molecular virology." Trends in Biochemical Sciences 19, no. 10 (October 1994): 433. http://dx.doi.org/10.1016/0968-0004(94)90095-7.

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Dissertations / Theses on the topic "Molecular virology"

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Fowotade, Adeola. "Molecular virology of KSHV : elucidating vIRF2 and vIRF4 function." Thesis, University of Surrey, 2017. http://epubs.surrey.ac.uk/813233/.

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The innate type I interferon antiviral response is the first line of defence invoked to limit the spread of viral infections. Hence a number of viruses including Kaposi's sarcoma-associated herpesvirus (KSHV) have evolved defence strategies against this antiviral response. KSHV is the aetiologic agent of KS and almost one quarter of the KSHV genes specify either demonstrated or potential immunomodulatory activity including the four viral interferon regulatory factors (vIRFs). vIRFs 1, 2 and 3 have previously been shown to inhibit type I IFN signalling, whereas the inhibitory role of vIRF4 is yet to be reported. A previous stable isotope labelling of amino acids in cell culture (SILAC) study coupled to LC-MS/MS identified USP7 and ribosomal proteins as binding partners of both vIRF2 and vIRF4. The aim of the present study was to investigate the role of these binding partners in type I IFN signalling and to determine the regulatory mechanisms behind the effects of these partner proteins on the functions of the vIRF2 and vIRF4 proteins. Polysome profiling and microarray studies were carried out on vIRF4 expressing cells and suggested a novel regulatory role for vIRF4 in translation. USP7 was also characterised as a positive regulator of IFN signalling and the mechanism behind this effect was explored.
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Sanfilippo, Luiz Francisco. "Epidemiologia e caracterização molecular do vírus da Influenza em quatro espécies de pinguins na Região Antártica." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-11082011-105843/.

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O Vírus da influenza, apesar de todas as epidemias e pandemias referirem-se a infecções em seres humanos, não está restrita a espécie humana e é capaz de causar debilidade ou mortalidade em várias outras espécies, incluindo cavalos, suínos, mamíferos marinhos e aves, entre outros. Estudos ecológicos das viroses de influenza conduziram a hipótese que todas as que acometem mamíferos derivam de reservatórios destes vírus em aves. Mesmo com programas de monitoramento contínuo de aves silvestres em alguns países do mundo que possuem casos originados pelos vírus aviário H5N1, pouco foi feito na Antártica e por isso, o presente trabalho foi realizado nas estações de verão antártico de 2006, 2007 e 2008 em duas localidades no território Antártico, a Península Keller, localizada na Ilha Rei George e na ilha Elefante 61°08S, 55°07W, a primeira onde está situada a Estação Antártica Comandante Ferraz-EACF e a segunda onde está localizada uma base de apoio a estudos avançados. Para este estudo foi realizada a coleta de 283 amostras de quatro diferentes espécies de pinguins: Pygoscelis adeliae; P. papua; P. antarctica; Aptenodytes patagonicus. Para o diagnóstico das amostras colhidas, foi aplicada a detecção direta dos produtos amplificados pelo método de RT-PCR em gel de agarose confirmados pelo método de Real-Time PCR (Applied Biosystems) e pelo RT-PCR-GeneScan no laboratório de Virologia Clínica e Molecular, do Departamento de Microbiologia, da Universidade de São Paulo. Os resultados obtidos em nosso estudo foram 8 amostras positivas em pinguins para o vírus Influenza A. As amostras positivas por RT-PCR foram encaminhadas para o laboratório de Influenza do Department of Infectious Diseases, St. Jude Children\'s Research Hospital, Memphis, TN, USA, para isolamento em ovos embrionados, não havendo crescimento de vírus da influenza A. Quatro destas amostras positivas puderam ser sequenciadas e comparadas com sequências de Influenza A depositadas no Genbank apresentando uma identidade de 96,8 % a 100 % entre elas e o controle tendo este último uma identidade de 100% com as do banco de dados, confirmando a presença do vírus nestas aves.
Epidemics and pandemics of influenza usually refer to infections in human beings. The influenza virus is not, however, restricted to humans and can cause infirmity and death in other species including horses, swine, marine mammals, birds, and others. Ecological studies of viral infections have led to the hypothesis that the influenza viruses that attack mammals have their origin in the accumulation of these viruses in birds (avian flu). In some countries with influenza cases caused by the avian H5N1 virus, there was monitoring of wild birds but little had been done in Antarctica. The present work was therefore carried out during the Antarctic summer seasons of 2006, 2007, and 2008 in two Antarctic locations: The Commander Ferraz Antarctic Station, on the Keller Peninsula of King George Island, and at the Base of Advanced Studies located on Elephant Island (61°08S, 55°07W). Two hundred eighty-three (283) samples from four different penguin species Pygoscelis adeliae, Pygoscelis papua, Pygoscelis antarctica; and Aptenodytes patagonicus were collected for this study. Diagnoses of the samples were performed not only by application of direct detection and amplification according to the RT-PCR method in agar-gel, but also by Real-Time PCR (Applied Biosystems), and by RT-PCR gene scan at the Laboratory of Clinical and Molecular Virology of the Department of Microbiology of the University of Sao Paulo. Eight of the penguin samples tested positive for the Influenza-A virus. The positive samples, as determined by RT-PCR, were sent to the Influenza Laboratory of the Department of Infectious Diseases of the St. Jude Research Hospital in Memphis, Tennessee, USA, to be isolated in egg embryos where no further growth of the Influenza-A virus took place. Four of these positive samples could be sequenced and compared with those of Influenza-A on deposit at the Gene Bank and ranged from 96.85 to 100% when compared with the control samples (100% positive), thus confirming the presence of the virus in the tested birds.
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Hughes, Fiona Lesley. "Molecular investigations of subgroup I geminiviruses." Doctoral thesis, University of Cape Town, 1991. http://hdl.handle.net/11427/21979.

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Bibliography: pages 139-150.
The diversity of Subgroup I geminiviruses causing streak disease in maize, sugarcane, and indigenous wild grasses was investigated. The virus. isolates studied originated from maize (several southern African isolates), two sugarcane cultivars (from Natal province, South Africa, and from Mauritius), wheat, and three grasses (Panicum, Setaria, and Eleusine spp. from South Africa). The following methods were used: analysis of restriction fragment length polymorphisms (RFLPs) between viral genomes in individual infected plants; DNA cross-hybridization between virus isolates; restriction endonuclease mapping of whole virus genomes; and nucleic acid sequencing. The complete genome of the Natal sugarcane streak virus isolate was sequenced. Partial sequences were obtained for other isolates, either by sequencing the ends of cloned viral genomes, or by sequencing a 250 base pair fragment of a highly conserved open reading frame that had been amplified using the polymerase chain reaction technique. The viruses being studied were compared both among themselves and with other Subgroup I geminiviruses of known DNA sequence, on the basis of sequence (nucleotide and amino acid) and restriction map data. Distance matrix methods were used to infer phylogenetic relationships between Subgroup I geminiviruses from restriction map and sequence data. Phylogenies deduced from sequence data were considered to be more accurate than those deduced from map data. Regardless of the method of analysis used, however, the relationships between the Subgroup I geminiviruses studied here remained constant. Thus, three strains of MSV (maize, Setaria, and Eleusine strains) were distinguished. Streak viruses distinct from MSV were also identified: panicum streak virus (PanSV), and two distantly related strains (Natal and Mauritius) of sugarcane streak virus (SSV). Restriction mapping of different geographical isolates of the maize strain of MSV demonstrated that variation existed within a single strain of virus. RFLP analysis indicated that minor variation existed between virus genomes within single diseased plants. Methods used to. type Subgroup I geminiviruses were evaluated, and discrepancies in the serological typing of geminiviruses from Subgroups I and III were pointed out. A unified scheme was proposed for distinguishing between distinct Subgroup I geminiviruses and strains of geminiviruses. The origins of maize and sugarcane streak viruses were speculated upon.
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Rizotto, Laís Santos. "Metapneumovírus aviários em aves silvestres." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-17052017-125020/.

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Metapneumovírus Aviário (aMPV), família Pneumoviridae, gênero Metapneumovírus, é o agente etiológico responsável pela rinotraqueíte dos perus e também está associada à síndrome da cabeça Inchada em galinhas, duas importantes doenças respiratórias que acometem aves comerciais e levam a grandes perdas econômicas. O objetivo deste estudo foi detectar a presença de aMPV em amostras de aves silvestres e realizar a caracterização molecular dos isolados encontrados, com o intuito maior de contribuir para o entendimento da epidemiologia desse vírus. Para isso foram coletados no total, 448 suabes orofaringeanos (OP) e cloacais (C) oriundos de 234 aves silvestres em quatro locais do estado de São Paulo. Três tipos de amostras foram processadas e testadas: 1) 266 suabes agrupados de um ou até cinco animais que estavam no mesmo recinto e respeitando o mesmo tipo de suabe (OP ou C); 2)188 suabes foram agrupados na forma de pools de até dois animais, contendo os suabes OP e C de cada animal, formando então 48 pools; 3) amostras de tecido traqueal e pulmonar de três Egretta thula também foram coletados e testados. A purificação de RNA viral foi realizada utilizando o QIAmp RNA Mini Kit Kit (Qiagen). Para o teste de RT-PCR foi utilizado o kit OneStep RT-PCR (Qiagen) com primers baseados no gene N, previamente descritos, com fragmento esperado de 115 bp. As amostras foram também testadas por RT-PCR em tempo real (RRT-PCR) com primers específicos previamente descritos, para os subtipos A e B, com base no gene G com fragmentos de 116 e 135 bp, respectivamente. Os fragmentos das amostras positivas foram purificados e sequenciados pelo sequenciamento tipo Sanger para caracterização por análises filogenéticas. Das 126 amostras testadas pelo teste de RT-PCR baseado no gene N, quatorze foram positivas: oito amostras de Anseriformes (Aix sponsa, Aix galericulata, Dendrocygna viduata), três de Columbiformes (Columba livia), um de Falconiformes (Falco sparverius), um de Psittaciformes (Psittacara leucophthalma) e um de Pelecaniformes (Egretta thula). Das quatorze amostras positivas, treze eram provenientes de suabes, e a décima quarta era oriunda de tecido traqueal de Egretta thula. Pelo teste de RRT-PCR baseado no gene G nenhuma das 184 amostras testadas foi positiva. As análises filogenéticas realizadas com fragmentos de 44 e 54 bp do gene N de duas amostras positivas, que se agruparam com isolados pertencentes ao aMPV de subtipo A. Estes vírus apresentaram alta identidade com as estirpes derivadas de vacina e com estirpes vacinais, o que mostra uma possível ocorrência de escapes vacinais de aves de produção para as aves silvestres.
Avian metapneumovirus (aMPV), family Pneumoviridae, genus Metapneumovirus, it is the etiologic agent responsible for turkey rhinotracheitis and is also associated with swollen head syndrome in chickens, two important respiratory diseases in poultry which leads to large economic losses. The aim of this study was detect the presence of aMPV in wild birds samples, to perform the phylogenetic analysis of the isolates found with the major objective of contributing to the understanding of the epidemiology of this virus in poultry farms. In total, 448 oropharyngeal (OP) and cloacal (C) swabs from 234 wild birds collected in four different locations within the state of São Paulo. The samples were processed and tested in three different ways: 1) 266 swabs were in the form of pools of one up to five animals that were in the same enclosure and respecting the same type of swab (OP or C); 2) 188 remaining swabs were grouped into pools of up to two animals, containing the oropharyngeal and cloacal swabs of each animal; 3) tracheal and pulmonary tissue samples were also collected and tested. Purification was performed using the QIAmp RNA Mini Kit Kit (Qiagen). Viral detection was performed by conventional RT-PCR technique using the OneStep RT-PCR kit (Qiagen) with primers based on the N gene, previously described with expected fragment of 115 bp. The samples were also tested by a real time RT-PCR (RRT-PCR) with specific primers previously described, for subtypes A and B, based on the G gene with fragments of 116 and 135 bp, respectively. Of the 126 samples tested by the RT-PCR N gene based, fourteen were positive: eight samples of Anseriformes (Aix sponsa, Aix galericulata, Dendrocygna viduata), three Columbiformes (Columba livia), one Falconiformes (Falco sparverius), one Psittaciformes (Psittacara leucophtalma) and one Pelecaniformes (Egretta thula). Of the swab samples, five were derived from oropharyngeal swabs and four from cloacal swabs, the other four samples were detected in the samples processed in pools of up to two animals, which contained the oropharyngeal and cloacal swabs of each bird. The positive Egretta thula sample was from a tracheal tissue sample. Based on the RRT-PCR G gene based, none of the 184 samples tested were detected. Phylogenetic analyzes were performed on two positive samples that proved to belong to aMPV subtype A, showing high similarity with the strains derived from the vaccine and with vaccine strains.
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Kapoor, Sanjay. "Molecular determinants of rotavirus virulence." Thesis, University of Warwick, 1995. http://wrap.warwick.ac.uk/4250/.

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Rotaviruses are the single most important etiological agent of severe diarrhoea in infants and young children in both developed and developing countries. The World Health Organisation has identified the development of a rotavirus vaccine as a priority area for routine childhood immunisation to control rotavirus infections. However, the candidate vaccine strains have not been very successful. The main aim of this project was to map rotavirus virulence to its gene segments. Such studies can help in developing better vaccines for the control of rotavirus induced diarrhoea. A three step approach was undertaken (i) development of an animal model, (ii) construction and characterisation of reassortants between rotavirus strains of different virulence, (iii) mapping virulence to rotavirus gene segments. The mouse model developed revealed that the outcome of rotavirus infection was influenced by viral dose and viral strain as well as by host age and host strain. Homologous murine rotavirus strain was found to be most virulent. Among the heterologous strains studied, the OSU strain was found to be most virulent and UKtc strain the least virulent. The CD- 1 strain of mouse was found to be the most susceptible to virus infection and C57/BL the least susceptible. A very simple and rapid nucleic acid extraction method has been developed that requires only one centrifugation step and circumvents the use of any hazardous organic chemicals, which can be applied to very large numbers of samples saving time and labour. Rotavirus reassortants were constructed in a variety of ways and their genotype determined from relative mobility of their gene segments on polyacrylamide gels and restriction enzyme digestion of PCR amplified products. Twenty two reassortants (2%) were identified out of more than 1100 progeny clones examined and these reassortants belonged to 15 different genotypes. Possible reasons for obtaining this low number of reassortants are discussed. No reassortant could be identified between a murine rotavirus and other heterologous rotavirus strains. Preliminary sequence of VP7 gene of murine rotavirus strains, EDIM and EBR, was found to be different to the published rotavirus sequences including the recently published five murine rotavirus strains. The virulence mapping studies conducted in mice with some of the 22 reassortants obtained in the present study showed that gene 4 of the OSU and UKtc strains was involved in virulence. Segment 5 of OSU strain and segments 5, and 8 of UKtc strain may also be involved in virulence.
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Janowicz, Anna Agata. "Molecular determinants of bluetongue virus virulence." Thesis, University of Glasgow, 2015. http://theses.gla.ac.uk/6959/.

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Bluetongue virus (BTV) is an arbovirus and the cause of “bluetongue”, a major infectious disease of ruminants. Whilst the BTV structure and replication strategies are well elucidated, less is known on the genetic variability of BTV and the molecular determinants affecting virus-host interactions. In order to investigate the determinants of BTV virulence, in this study, we compared the phenotype and genotype of a highly virulent strain of BTV-8 isolated in the Netherlands a passaged minimally in tissue culture (BTV8L), with a strain passaged extensively in tissue culture (BTV8H). BTV8L was shown to be highly pathogenic in sheep and in a mouse model of bluetongue, while BTV8H was attenuated in both hosts. Full genome sequencing revealed differences in 16 amino acid residues between these two strains. Using reverse genetics, we rescued both viruses, in order two further dissect their biological features. Rescued viruses retained the phenotype of the parental viruses in vivo and in vitro. Reassortants between BTV8L and BTV8H showed that mutations in several segments contributed to attenuation of the high passage virus. The major determinants of BTV8 virulence in IFNAR-/- mice were shown to be located in segments 1, 2, 6 and 10. In vitro studies of selected reassortants showed that through extensive passage in tissue culture BTV8H acquired increased affinity for glycosaminoglycans. This property was conferred by mutations in segment 2 and resulted in increased yields of the virus in vitro and attenuation in vivo. Additionally, BTV8H was unable to replicate in IFN competent primary sheep endothelial cells. Our data showed that multiple segments were involved in decreased efficiency of BTV8H replication in cells in an IFN-induced antiviral state. Moreover, we examined changes in viral population diversity that occured after BTV-8 isolation in insect cells (Culicoides, KC) and after passage in mammalian cells and linked decreased diversity with BTV virulence in vivo. We found, that in general, the number of genetic variants was higher in BTV-8 before cell passaging, or after one passage in KC cells, compared to the number observed after even a single passage in BHK-21 cells. The highest total number of variants was found in virus passaged in KC cells, which suggests that insect vector might serve as an amplifier of quasispecies diversity of BTV. Together, these findings suggest that the virulence of BTV is a multifactorial phenomenon involving many aspects of virus-host interactions and it is not only affected by changes in the viral proteins selected at the consensus level, but also by the genetic variability of the population as a whole.
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Slack, Gillian Sinclair. "Molecular and biological characterisation of orthobunyaviruses." Thesis, University of Glasgow, 2016. http://theses.gla.ac.uk/7303/.

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Orthobunyaviruses are the largest genus within the Bunyaviridae family, with over 170 named viruses classified into 18 serogroups (Elliott and Blakqori, 2001; Plyusnin et al., 2012). Orthobunyaviruses are transmitted by arthropods and have a tripartite negative sense RNA genome, which encodes 4 structural proteins and 2 non-structural proteins. The non-structural protein NSs is the primary virulence factor of orthobunyaviruses and potent antagonist of the type I interferon (IFN) response. However, sequencing studies have identified pathogenic viruses that lack the NSs protein (Mohamed et al., 2009; Gauci et al., 2010). The work presented in this thesis describes the molecular and biological characterisation of divergent orthobunyaviruses. Data on plaque morphology, growth kinetics, protein profiles, sensitivity to IFN and activation of the type I IFN system are presented for viruses in the Anopheles A, Anopheles B, Capim, Gamboa, Guama, Minatitlan, Nyando, Tete and Turlock serogroups. These are complemented with complete genome sequencing and phylogenetic analysis. Low activation of IFN by Tete serogroup viruses, which naturally lack an NSs protein, was also further investigated by the development of a reverse genetics system for Batama virus (BMAV). Recombinant viruses with mutations in the virus nucleocapsid protein amino terminus showed higher activation of type I IFN in vitro and data suggests that low levels of IFN are due to lower activation rather than active antagonism. The anti-orthobunyavirus activity of IFN-stimulated genes IFI44, IFITMs and human and ovine BST2 were also studied, revealing that activity varies not only within the orthobunyavirus genus and virus serogroups but also within virus species. Furthermore, there was evidence of active antagonism of the type I IFN response and ISGs by non-NSs viruses. In summary, the results show that pathogenicity in man and antagonism of the type I IFN response in vitro cannot be predicted by the presence, or absence, of an NSs ORF. They also highlight problems in orthobunyavirus classification with discordance between classical antigen based data and phylogenetic analysis.
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Fernandez, Llenalia Garcia. "Statistical modelling of performance data for molecular amplification methods in diagnostic virology." Thesis, University of Abertay Dundee, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.650529.

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Nucleic Acid Technology (NAT), introduced in the late 90s, is a molecular amplification method that can be used for the diagnosis and management of patients with infectious diseases. NAT test results are obtained quicker and are quantified, providing great.er information than the positive/negative results available from traditional techniques. However, NATs arc technically demanding, susceptible to contamination and hence results from associated diagnostic tests may be inaccurate. External Quality Assessment (EQA) services are programmes developed to assess and advance the quality performance of laboratories that use NAT kits to diagnose, manage and control human diseases. Quality Control for Molecular Diagnostics (QCMD) , an organisation that provides EQA, uses proficiency panels designed with samples containing no , weak, medium and strong microbial loads. The panels are distributed to participating laboratories who analyse them knowing the pathogen but blind to the microbial load.
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Hunt, Nicholas. "Molecular analysis of the Friend virus complex." Thesis, University of Warwick, 1989. http://wrap.warwick.ac.uk/108015/.

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This work was undertaken to molecularly clone the Nirand strain of the polycythaemia inducing Friend spleen focus forming virus (F-SFFVp) together with its replication competent helper virus. Friend murine leukaemia virus (F-MuLV). To this aim viral extrachromosomal DNA molecules (of both linear and circular nature corresponding to both of these viruses) which could be induced in Friend cell lines were molecularly characterised with respect to their quantitative increase during differentiation and subcellular location. One cell line, F4-6 in which considerable amounts of these extrachromosomal DNAs could be detected was utilised for the large scale production and purification of both SFFVp and F-MuLV extrachromosomal DNA molecules. Restriction enzyme analysis of such molecules in combination with Southern blotting enabled the construction of primary restriction enzyme maps which allowed the selection of a molecular cloning strategy. Both of these viruses were subsequently molecularly cloned and were subsequently shown to be biologically active after transfection into recipient cells. A further aspect of this work was to construct a biologically active SFFVp containing a dominant selectable marker gene. A selectable construct was generated by joining different regions of the genome of the myeloproliferative sarcoma virus (MPSV) and SFFVp. A construct with the U3 region from the long terminal repeat (LTR) of SFFVp and the envelope gene region (gp55) of SFFVp (designated neo2 SFFVp) was found to be fully active as a selectable retroviral vector with identical biological properties to the wild type SFFVp neo2 SFFVp induced erythroid differentiation in vivo, with infected cells no longer requiring erythropoietin for differentiation. Furthermore neo2 SFFVp infected spleen cells could be used to generate immortal Friend leukaemia cells which were selectable with geneticin (neo2). A further construct, neo2 SFFV-M, which had a U3 region originating from NPSV was able to cause erythropoietin independent erythroid differentiation. However as compared to neo2 SFFVp and indeed the wild type SFFVp, neo2 SFFV-M was able to induce the erythroproliferative disease with a different kinetics. All attempts to isolate transformed Friend cells with this construct failed.
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Yadav, Sarita. "Isolation and molecular characterization of bluetongue virus from Southern India." Thesis, University of Glasgow, 2018. http://theses.gla.ac.uk/30807/.

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BT is endemic in India, particularly in the South and BTV strains belonging to twelve BTV-serotypes (BTV-1, 2, 3, 4, 5, 9, 10, 12, 16, 21, 23 and 24) have been isolated since 2001. A pentavalent inactivated BTV vaccine containing serotypes 1, 2, 10, 16 and 23, is currently being used in the South. This study focusses on the isolation and the typing of BTV strains from Southern India, 2014-15, then generation of full length sequence data, primarily for BTV genome Seg-2 to provide molecular epidemiology information concerning the strains circulating in the region. Suspected field outbreaks of BTV from Telangana, Andhra Pradesh, Karnataka and Tamil Nadu states in Southern India during 2014-2015 were attended to collect well documented blood and necropsy clinical samples from mixed population of small ruminants and cattle. Of the 447 field samples (EDTA blood, spleen, lymph node and saliva), 236 gave positive results for BTV RNA by serogroup-specific real-time RT-PCR targeting Seg-9 and Seg-10 for 2014 and 2015 samples respectively. A total of 141 BTV virus isolations were made from 141 serogroup-specific qRT-PCR positive samples, by infecting KC and BHK cells. The isolated 63 BTVs were serotyped by qRT-PCR assay targeting Seg-2 of the BTV-1 to BTV-24, BTV-26, BTV-27 and BTV-29, confirming the current co-circulation of 7 BTV serotypes, including BTV-1, BTV-2, BTV-4, BTV-5, BTV-9, BTV-10 and BTV-12 in 62 BTV isolates. Dual and triple BTV co-infections with these serotypes were detected in 22 and 4 BTV isolates, respectively. None of the serotypes present in the pentavalent vaccine were identified in any of the 22 BTV isolates from vaccinated animals, suggesting that the vaccine was indeed effective against the homologous serotype field strains. Among the 7 serotypes identified by Seg-2 based ORF sequence analysis of 46 BTV isolates (BTV-1, BTV-2, BTV-4, BTV-5, BTV-9, BTV-10 and BTV-12), 4 serotypes BTV-1, BTV-2, BTV-4 and BTV-9, belonged to eastern topotypes, whereas 3 serotypes, BTV-5, BTV-10 and BTV-12 were identified as western topotypes. For 4 BTV isolates, the serotype detected by qRT-PCR was different of that detected by NGS. The full genome sequencing and analyses is still ongoing in Glasgow. This data will assist in understand the epidemiology of the BT in India and implementation of effective BTV control to ensure the use of appropriate BTV serotype and strains in the available BT vaccine in India.
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Books on the topic "Molecular virology"

1

R, Kinchington Paul, ed. Molecular virology. 2nd ed. Oxford: BIOS Scientific Publishers, 1998.

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Harper, D. R. Molecular virology. 2nd ed. New York: Springer, 1998.

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Collins, Mary K. Practical Molecular Virology. New Jersey: Humana Press, 1991. http://dx.doi.org/10.1385/0896031918.

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Weller, Sandra K. Alphaherpesviruses: Molecular virology. Norfolk, UK: Caister Academic Press, 2011.

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W, Davies Jeffrey, ed. Molecular plant virology. Boca Raton, Fla: CRC Press, 1985.

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W, Adolph Kenneth, ed. Molecular virology techniques. San Diego: Academic Press, 1994.

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1944-, Adolph Kenneth W., ed. Molecular virology techniques. San Diego: Academic Press, 1994.

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Cann, Alan. Principles of molecular virology. San Diego, CA: Academic Press, 1993.

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Jane, Flint S., and American Society for Microbiology, eds. Principles of virology. 3rd ed. Washington, DC: ASM Press, 2009.

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J, Davison Andrew, and Elliott Richard M, eds. Molecular virology: A practical approach. Oxford: IRL Press, 1993.

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Book chapters on the topic "Molecular virology"

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Boland, G. J. "Virology." In Molecular Diagnostics, 33–50. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-4511-0_3.

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Wu, Josephine, Mona Sharaan, and David Y. Zhang. "Molecular Virology." In Molecular Genetic Pathology, 533–80. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-405-6_21.

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Ye, Fei, Miao Cui, Rame H. Khasawneh, Robert Shibata, Josephine Wu, Mona Sharaan, and David Y. Zhang. "Molecular Virology." In Molecular Genetic Pathology, 655–731. New York, NY: Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4614-4800-6_25.

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zu Siederdissen, Christoph Höner, Markus Cornberg, and Michael P. Manns. "Clinical Virology: Diagnosis and Virologic Monitoring." In Molecular and Translational Medicine, 205–16. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-22330-8_10.

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Modrow, Susanne, Dietrich Falke, Uwe Truyen, and Hermann Schätzl. "Historical Overview." In Molecular Virology, 3–15. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-20718-1_1.

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Modrow, Susanne, Dietrich Falke, Uwe Truyen, and Hermann Schätzl. "Vaccines." In Molecular Virology, 135–46. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-20718-1_10.

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Modrow, Susanne, Dietrich Falke, Uwe Truyen, and Hermann Schätzl. "Epidemiology." In Molecular Virology, 147–54. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-20718-1_11.

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Modrow, Susanne, Dietrich Falke, Uwe Truyen, and Hermann Schätzl. "Viral Evolution." In Molecular Virology, 155–61. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-20718-1_12.

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Modrow, Susanne, Dietrich Falke, Uwe Truyen, and Hermann Schätzl. "Laboratory Methods for Detecting Viral Infections." In Molecular Virology, 163–81. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-20718-1_13.

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Modrow, Susanne, Dietrich Falke, Uwe Truyen, and Hermann Schätzl. "Viruses with Single-Stranded, Positive-Sense RNA Genomes." In Molecular Virology, 185–349. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-20718-1_14.

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Reports on the topic "Molecular virology"

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Comunicación de las Ciencias, Centro. Pandemia de COVID-19: Lecciones desde España. Universidad Autónoma de Chile, March 2020. http://dx.doi.org/10.32457/2050012728/9607202041.

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Abstract:
Tanto Chile como España están enfrentando junto con la Pandemia de COVID-19, una “infodemia”, término utilizado por la Organización Mundial de la Salud (OMS) para referirse a la sobreabundancia de información falsa y su rápida propagación. En medios españoles el neurovirólogo José Antonio López Guerrero (JAL), divulgador y Profesor Titular del Departamento de Biología Molecular de la Universidad Autónoma de Madrid (UAM), es un invitado frecuente para aclarar las dudas de la ciudadanía y combatir las “Fakes News”, porque además de investigar antivirales contra coronavirus en su laboratorio, es uno de los responsables de comunicación de la Sociedad Española de Virología. En entrevista con el Centro de Comunicación de las Ciencias, el científico español nos aclaró algunas dudas respecto al nuevo coronavirus o como él le llama “el pequeño jinete del apocalipsis”.
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