Academic literature on the topic 'Molecular markers; Plant genetics; DNA'

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Journal articles on the topic "Molecular markers; Plant genetics; DNA"

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Szczechura, Wojciech, Mirosława Staniaszek, and Hanna Habdas. "Tomato Molecular Markers." Vegetable Crops Research Bulletin 74, no. 1 (January 1, 2011): 5–23. http://dx.doi.org/10.2478/v10032-011-0001-y.

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Tomato Molecular MarkersTomato (Solanum lycopersicumL.) is one of the most popular vegetable grown in many regions of the world. Due to its high taste quality and nutritional value increase interest in the cultivation of this species and its consumption. Using the latest achievements in fields of genetics, molecular biology and biotechnology, breeders can create new varieties with improved useful traits. Introduction of DNA markers, especially those based on the polymerase chain reaction (PCR) has led to breakthrough in the plants genetic research, including tomato. They are successfully used for plant genomes mapping, phylogenetics studies, selection of parental forms in plant breeding, and above all to identify the genes of important traits. For tomato have been identified and mapped 9309 molecular markers. High-density genetic maps development gives an opportunity to use them in genetic research and breeding programs. Identification of DNA markers closely linked to studied gene can significantly facilitate the identification of desirable traits in material breeding, or accelerate the plants selection for elimination of genotypes with undesirable genes. Material breeding selection using molecular markers, defined as MAS (marker-assisted-selection) is increasingly being used in tomato breeding programs, contributing to facilitated identification of genes or QTL and their transfer into the cultivated species from wild form.
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Nam, Vu Tuan, Pham Le Bich Hang, Nguyen Nhat Linh, Luu Han Ly, Huynh Thi Thu Hue, Nguyen Hai Ha, Ha Hong Hanh, and Le Thi Thu Hien. "Molecular markers for analysis of plant genetic diversity." Vietnam Journal of Biotechnology 18, no. 4 (May 24, 2021): 589–608. http://dx.doi.org/10.15625/1811-4989/18/4/15326.

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Genetic diversity plays an important role in diversity conservation at multiple levels and supports to monitor and assess genetic variation. In plants, genetic diversity provides the ability to adapt and respond to environmental conditions that helps plants to survive through changing environments. Genetic diversity analyses based on molecular genetic markers are effective tools for conservation and reintroduction of rare and endangered species. In recent years, the development of various chemical and molecular techniques for studying genetic diversity has received great attention. While biochemical markers are primarily used in the diagnosis of pathogens, DNA markers have been developed and widely applied for identification of species and population based on the genotype of an organism that is more stable and not easily affected by the environmental factors. PCR-based molecular marker tools, such as restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNA (RAPD), simple sequence repeats (SSRs) are used for analysing the difference in the targeted DNA sequences. With the rapid and robust development of genomic sequencing technology it is now possible to obtain and analyse DNA sequences of the whole genome of studied organisms. However, each type of DNA markers has different principles, as well as the pros and cons of specificity. In this article, we review methods and point out DNA markers, which are considered as reliable and widely used tools for the detection of genetic variation. In addition, we present the application of DNA marker in analysing genetic diversity of wild, domestic and medicinal plants, as well as some perspectives on the future of DNA marker’s application in the analysis of genetic diversity.
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Chesnokov, Yu V. "GENETIC MARKERS: COMPARATIVE CLASSIFICATION OF MOLECULAR MARKERS." Vegetable crops of Russia, no. 3 (July 25, 2018): 11–15. http://dx.doi.org/10.18619/2072-9146-2018-3-11-15.

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With the creation of the molecular markers allowing to carry out analysis of genotypes on the level initial genetic information – DNA, onset one of the most multifarious and one of the most large in number class of markers at the present day. It is concerned with that each separate nucleic acid sequence is unique on its structure. Set of molecular and genetic methods, named as DNA-fingerprinting, most wide used in modern investigations for solving different problems in different biological areas. In this connection, necessity in comparative classification of modern molecular and genetic markers is actual. Based on published literature material it shown data on different classifications of molecular markers. Determined definition of term “marker” in genetics and breeding. Gave the characters and distinctive features of genetic markers. It given the definition what is “good” genetic marker as well as kinds, categories, variations and types on heredity of molecular markers. Manifested by means of molecular markers polymorphisms can classified on polymorphism of sequence itself (including nucleotide substitution and insertion-deletion) and polymorphism the number of tandem repeat sequences in repeated regions. Moreover, molecular markers can classify on two variations: anonymous, for which nucleotide acid sequence unknown and for manifestation of the molecular marker its detection not necessary (for example, RAPD, AFLP, RFLP), and announce (or determined), for which nucleic acid sequence is known or can be detect during analysis (for example, SNP, CAPS, STS). However, in independence on using of molecular markers the choice of method of investigation will be depend on investigated plant species as well. The next influence of molecular and genetic methods on genetics and practical breeding of plants will be depend on results, which will be obtain, in particular, on revealing the possibility or not possibility of genotyping of individual on single genetic marker as wel as on economic price of obtain informative data.
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Pourmohammad, Alireza. "Application of molecular markers in medicinal plant studies." Acta Universitatis Sapientiae, Agriculture and Environment 5, no. 1 (December 1, 2013): 80–90. http://dx.doi.org/10.2478/ausae-2014-0006.

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Abstract The World Health Organization has estimated that more than 80% of the world’s population in developing countries depends primarily on herbal medicine for basic healthcare needs. Approximately two thirds of the 50 000 different medicinal plant species in use are collected from the wild and only 10% of medicinal species used commercially are cultivated. DNA-based molecular markers have utility in the fields like taxonomy, physiology, embryology, genetics, etc. DNA-based techniques have been widely used for authentication of plant species of medicinal importance. The geographical conditions affect the active constituents of the medicinal plant and hence their activity profiles. Many researchers have studied geographical variation at the genetic level. Estimates of genetic diversity are also important in designing crop improvement programmes for the management of germplasm and evolving conservation strategies. The DNA-based molecular marker helps in the improvement of medicinal plant species. DNA markers are more reliable because the genetic information is unique for each species and is independent of age, physiological conditions and environmental factors.
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Xuan, Zhou, Hong Dao Zhang, Zheng Hong Li, Cheng Zhang, Ji Lin Li, and Yan Ming Zhang. "The Role of Molecular Marker in Development of Plant Genetic Resources." Advanced Materials Research 955-959 (June 2014): 855–58. http://dx.doi.org/10.4028/www.scientific.net/amr.955-959.855.

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Plants are fundamental to life, being the basis of our food production and an essential part of the global ecosystem on which life on earth depends. Plant genetic resources include primitive forms of cultivated plant species and landraces, modern cultivars, breeding lines and genetic stocks, weedy types and related wild species, which provide the building blocks that, allow classical plant breeders and biotechnologists to develop new commercial varieties and other biological products. Detection and analysis of genetic variation can help us to understand the molecular basis of various biological phenomena in plants. Molecular markers for the detection and exploitation of DNA polymorphism is one of the most significant developments in the field of molecular genetics. The presence of various types of molecular markers, and differences in their principles, methodologies, and applications require careful consideration in choosing one or more of such methods. This article describes the advances of molecular marker in present, introduces the molecular basis in development of plant genetic resources and perspectives the important role of molecular marker in development of plant genetic resources in the future.
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Zambelli, A. "THE IMPACT OF MOLECULAR GENETICS IN PLANT BREEDING: REALITIES AND PERSPECTIVES." Journal of Basic and Applied Genetics 30, no. 1 (July 2019): 11–15. http://dx.doi.org/10.35407/bag.2019.xxx.01.02.

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Even when conventional breeding was effective in achieving a continuous improvement in yield, Molecular Genetics tools applied in plant breeding contributed to maximize genetic gain. Thus, the use of DNA technology applied in agronomic improvement gave rise to Molecular Breeding, discipline which groups the different breeding strategies where genotypic selection, based on DNA markers, are used in combination with or in replacement of phenotypic selection. These strategies can be listed as: marker-assisted selection; marker-assisted backcrossing; marker assisted recurrent selection; and genomic selection. Strong arguments have been made about the potential advantages that Molecular Breeding brings, although little has been devoted to discussing its feasibility in practical applications. The consequence of the lack of a deep analysis when implementing a strategy of Molecular Breeding is its failure, leading to many undesirable outcomes and discouraging breeders from using the technology. The aim of this work is to trigger a debate about the convenience of the use of Molecular Breeding strategies in a breeding program considering the DNA technology of choice, the complexity of the trait of agronomic interest to be improved, the expected accuracy in the selection, and the demanded resources. Key words: DNA marker, selection, plant improvement.
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Chinnappareddy, L. R. D., K. Khandagale, A. Chennareddy, and V. G. Ramappa. "Molecular markers in the improvement of Allium crops." Czech Journal of Genetics and Plant Breeding 49, No. 4 (November 26, 2013): 131–39. http://dx.doi.org/10.17221/111/2013-cjgpb.

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The genus Allium (Family: Alliaceae) is the most important among the bulbous vegetable crops. characterization of Alliums based on phenotypic traits is influenced by the environment and leads to biased diversity estimates. Recognizing the potential of DNA markers in plant breeding, researchers have adopted the molecular markers for marker-assisted selection (MAS), quantitative trait loci (QTL) mapping and characterization of different quality traits in Alliums. This review presents details about the use of DNA markers in Alliums for cultivar identification, diversity studies, SSR development, colour improvement, total soluble solids (TSS), cytoplasmic male sterility (CMS) and efforts of DNA sequencing. As there are no such reports to describe the above work under a single heading, we decided to mine literature for those who are working in onion, garlic, chives and leek improvement to generate new insights in the subject.
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Rasmussen, Søren K. "Molecular Genetics, Genomics, and Biotechnology in Crop Plant Breeding." Agronomy 10, no. 3 (March 23, 2020): 439. http://dx.doi.org/10.3390/agronomy10030439.

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A diverse set of molecular markers techniques have been developed over the last almost 40 years and used with success for breeding a number of major crops. These have been narrowed down to a few preferred DNA based marker types, and emphasis is now on adapting the technologies to a wide range of crop plants and trees. In this Special Issue, the strength of molecular breeding is revealed through research and review papers that use a combination of molecular markers with other classic breeding techniques to obtain quality improvement of the crop. The constant improvement and maintenance of quality by breeding is crucial and challenged by a changing climate and molecular markers can support the direct introgression of traits into elite breeding lines. All the papers in this Special Issue “Molecular genetics, Genomics, and Biotechnology in Crop Plant Breeding” have attracted significant attention, as can be witnessed by the graphs for each paper on the Journal’s homepage. It is the hope that it will encourage others to use these tools in developing an even wider range of crop plants and trees.
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Lācis, Gunārs. "Characterisation of Latvia Fruit Crop Genetic Resources by Application of Molecular Genetics Methods." Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences 67, no. 2 (August 1, 2013): 84–93. http://dx.doi.org/10.2478/prolas-2013-0014.

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A large diversity of fruit crop accessions is maintained at the Latvia State Institute of Fruit- Growing, which consists of modern cultivars, landraces and selections from local breeding programmes, as well as germplasm that has resulted from scientific exchange and co-operation with other institutes. Presently, the germplasm collection comprises 2509 accessions of 17 fruit crops; 676 accessions are designated as national genetic resources. Conservation of germplasm itself has little value without characterisation and further utilisation of the stored plant material. To intensify these activities, DNA-based technologies have been implemented in the characterisation of germplasm. Two main groups of molecular markers have been utilised: non-specific markers and gene-specific (functional) markers, subsequently applicable for Marker Assisted Selection (MAS). Genotyping protocols based on SSR, RAPD and Methylation-sensitive amplification polymorphism (MSAP) markers have been developed for twelve fruit crops for use in plant material identification, True-to-Type verification and evaluation of genetic diversity and internal collection structure. In total, 790 accessions have been genotyped using any of the mentioned markers. These markers have been harmonised with the European cooperative programme for plant genetic resources working group (ECPGR WG) recommended sets to ensure international data exchange. Gene specific molecular markers have been applied to apple and pear (resistance to scab), strawberry (resistance to Gnomonia fragariae), sweet cherries and plums (self-incompatibility).
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McDonald, B. A., R. E. Pettway, R. S. Chen, J. M. Boeger, and J. P. Martinez. "The population genetics of Septoria tritici (teleomorph Mycosphaerella graminicola)." Canadian Journal of Botany 73, S1 (December 31, 1995): 292–301. http://dx.doi.org/10.1139/b95-259.

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The DNA-based markers of molecular genetics were combined with the analytical tools of population genetics to learn about the population biology of the wheat pathogen Mycosphaerella graminicola. DNA-based genetic markers, including restriction fragment length polymorphisms in nuclear and mitochondrial DNA, DNA fingerprints, and electrophoretic karyotypes were used in combination to show that the amount and distribution of genetic variation within and among field populations of M. graminicola is similar around the world. Measures of gametic disequilibrium suggested that the sexual stage of reproduction has a more significant impact on the genetic structure of M. graminicola populations than asexual reproduction. A field experiment conducted over a 3-year period showed that populations had a high degree of genetic stability over time. The potential effects of selection were quantified in a cultivar mixture experiment with four wheat cultivars that varied in resistance to M. graminicola. In combination, these experiments demonstrated the utility of selectively neutral genetic markers to elucidate the population genetics of fungi. Key words: genetic diversity, wheat, gene flow, RFLPs, DNA fingerprinting.
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Dissertations / Theses on the topic "Molecular markers; Plant genetics; DNA"

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Rendell, Sarah. "Population genetic structure of Faidherbia albida (Del.) A. Chev. (Leguminosae, Mimosoideae)." Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299160.

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Hochschartner, Gerald. "Revealing the past : the potential of a novel small nucleolar RNA (snoRNA) marker system for studying plant evolution." Thesis, University of St Andrews, 2011. http://hdl.handle.net/10023/1695.

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Despite the existence of various molecular marker systems there are still limitations in distinguishing between closely related species based on molecular divergence, especially when hybridization events have occurred in the past. The characterisation of plant small nucleolar RNA (snoRNA) genes and their organisation into multigene clusters provides a potential nuclear marker system which could help in resolving the phylogenetic history of plants and might be applicable in DNA barcoding. Using closely and distantly related Senecio species, I investigated a combination of fragment length and sequence variation of snoRNA genes/snoRNA gene clusters to assess the utility of this marker system for barcoding and resolving species relationships. SnoRNA gene and gene cluster sequences identified in Arabidopsis thaliana were used to find homologues in other species and subsequently used for the design of universal primers. Most of the universal primer pairs designed were successful in amplifying snoRNA fragments in most Senecio species and fragment length variation between and within species could be detected. Furthermore, the combination of some fragment length datasets produced by different primer pairs enabled the separation of species and the detection of reticulate evolution indicating a high potential of snoRNA gene/gene cluster fragment length polymorphisms (SRFLPs) for phylogenetic reconstructions in Senecio and other plant genera. Most of the examined gene clusters showed a similar gene order in Senecio and Arabidopsis. However, the majority of these clusters appeared to exhibit more copies in Senecio, some of which were distinguishable by a combined sequencing/fragment profiling approach, and shown to be putative single copy regions with the potential to be used as co-dominant markers. However, a high number of paralogues and possible differences in copy number between species excludes these regions from being used in DNA barcoding. This is because specific primers would have to be developed for specific copies which would preclude development of a universal application for barcoding. None of the regions showed enough sequence variation to delimit distinctly closely related Senecio species and were therefore also considered to be unsuitable for DNA barcoding. Although most snoRNA genes and gene clusters might be inapplicable for DNA barcoding, they are likely to be valuable for phylogenetic studies of species groups, genera and families. On this scale, specific primers might act universally and the number of paralogous copies is likely to be equal across the species group of interest.
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Bitalo, Daphne Nyachaki. "Implementation of molecular markers for triticale cultivar identification and marker-assisted selection." Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/71670.

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Thesis (MSc)--Stellenbosch University, 2012.
Triticale is an amphidiploid that consists of wheat (A and B) and rye (R) genomes. This cereal is fast becoming important on a commercial basis and warrants further assessment for the better management and breeding of the hybrid. The assessment of the genetic diversity among the wheat and rye genomes within triticale can be obtained by using molecular markers developed in both donor genomes. Simple sequence repeats markers (SSRs) and amplified fragment length markers (AFLPs) have been previously used to assess the genetic diversity among triticale lines. SSRs are highly polymorphic markers that are abundant and which have been shown to be highly transferable between species in previous studies while AFLP markers are known to generate plenty of data as they cover so many loci. Thus, the aim of this study was to develop a marker system suitable to assess the genetic diversity and relationships of advanced breeding material (and cultivars) of the Stellenbosch University’s Plant Breeding Laboratory (SU-PBL). Therefore, both AFLP and SSR markers were initially analysed using eight triticale cultivars (with known pedigrees) to facilitate cultivar identification. Fourty-two AFLP primer combinations and 86 SSR markers were used to assess the genetic diversity among the Elite triticale cultivars. The AFLP primer combinations generated under average polymorphism information content (PIC) values. Furthermore, these markers generated neighbour-joining (NJ) and unweighted pair group method with arithmetic average (UPGMA) dendograms that displayed relationships that did not correspond with the available pedigree information. Therefore, this marker system was found not to be suitable. A set of 86 SSRs previously identified in both wheat and rye, was used to test the genetic diversity among the eight cultivars. The markers developed in wheat achieved 84% transferability while those developed in rye achieved 79.3% transferability. A subset of SSR markers was able to distinguish the cultivars, and correctly identify them by generating NJ and UPGMA dendograms that exhibited relationships that corroborated the available pedigree data. This panel of markers was therefore chosen as the most suitable for the assessment of the advanced breeding material. The panel of seven SSR markers was optimised for semi-automated analysis and was used to screen and detect the genetic diversity among 306 triticale entries in the F6, Senior and Elite phases of the SU-PBL triticale breeding programme. An average PIC value of 0.65 was detected and moderate genetic variation was observed. NJ and UPGMA dendograms generated showed no clear groupings. However, the panel of markers managed to accurately identify all cultivars within the breeding program. The marker panel developed in this study is being used to routinely distinguish among the advanced breeding material within the SU-PBL triticale breeding programme and as a tool in molecular-assisted backcross.
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Kisiel, John B. "Discovery and validation of aberrantly methylated DNA markers of pancreatic cancer." Thesis, College of Medicine - Mayo Clinic, 2015. http://pqdtopen.proquest.com/#viewpdf?dispub=1584252.

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Introduction: Pancreatic adenocarcinoma is anticipated to become the most fatal gastrointestinal neoplasm by 2017. Because patients who present with symptoms uniformly have advanced disease, 5-year survival is poor. Surveillance programs in the highest risk patients have produced disappointing results and use invasive tests that would not be safe or practical at the population level. Molecular markers, specifically aberrant DNA methylation, show promise in early studies. We aimed to: 1) measure the sensitivity and specificity of aberrant DNA methylation in stools as a minimally invasive way to detect pancreatic cancer; and, 2) identify novel methylation candidate markers highly sensitive and specific for pancreatic cancer.

Methods: To select candidate markers among those reported in the literature, DNA was extracted from unmatched tissue samples of pancreatic cancers and of normal colonic epithelia and assayed for aberrant methylation by methylation specific PCR. The most discriminant candidates and mutant KRAS were then assayed from matched archival stools of patients with pancreatic cancer in comparison to stools from healthy controls. Sensitivity and specificity for pancreatic cancer were determined from multivariate logistic regression models. To identify novel candidate markers, DNA was extracted from matched, archival tissues of pancreas cancer and two control groups (normal pancreas and normal colon) and sequenced using the reduced representation bisulfite technique. Among all mapped regions, those with the highest variance in methylation differences between cases and controls were filtered prior to analysis. Significant regions were then blindly assayed by methylation specific PCR in an independent, matched sample set, where sensitivity and specificity were measured using univariate logistic regression.

Results: In tissues, methylated BMP3, EYA4, UCHL1 and MDFI were highly discriminant for pancreatic cancer in comparison to normal colon samples. However, when assayed in stools, only BMP3 remained significant. In combination with mutant KRAS, the area under the receiver operating characteristics curve for BMP3 in detection of pancreatic cancer, was 0.85, indicating strong association. Results were not significantly influenced by tumor location or stage. Reduced representation bisulfite sequencing identified over 500 differentially methylated regions which met a priori significance thresholds. The top 25 novel candidates were validated in independent samples, showing both strong association with cases, compared to controls and high signal to noise ratio.

Conclusions: We report the first demonstration of feasibility for the detection of pancreatic cancer using assay of aberrantly methylated DNA markers from stool. This is a critical first step in the long-term goal of developing a minimally invasive screening tool to curb the mortality rate of this devastating disease. Because the discriminant candidate markers, methylated BMP3 and mutant KRAS, are not unique to pancreatic cancer, we developed a marker discovery strategy which yielded dozens of highly discriminant, validated, novel candidates, many of which have never before been reported in association with cancer. Further studies are indicated to measure the site-specificity of these markers for pancreatic cancer, compared to other gastrointestinal neoplasms and to study the clinical utility of these novel candidates in distant biologic media, such as blood, stool or pancreatic juice.

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Helgason, Thorunn. "Molecular markers in conservation genetics : chlorolast DNA variation in natural Scottish Pinus sylvestris L." Thesis, University of Edinburgh, 1993. http://hdl.handle.net/1842/14044.

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Scottish P. sylvestris populations have been extensively researched, using monoterpene and isozyme markers, but differentiation among populations is too low to identify origins or gene flow. Protocols were developed for the analysis of chloroplast DNA (cpDNA) Restriction Fragment Length Polymorphisms (RFLP) in P. sylvestris to determine whether cpDNA markers could be used for these purposes. Nine populations from throughout the range of pine in Scotland were sampled. Two individuals from each of these populations were surveyed using seven restriction enzymes and 13 probes from P. contorta, a total of 91 probe/enzyme combinations. The cpDNA genome of P. sylvestris was found to be about 119 kilobase pairs in length. 100% of the length of the genome was sampled, and 0.52% of the sequence length. No variation was found in any individual. These results were compared with a survey of P. sylvestris from China, Sweden and Turkey. There is no evidence that the cpDNA genotype of Scottish pine differs in any way from these varieties, suggesting that the cpDNA genome of P. sylvestris is homogeneous over a large part of the species' range. A survey of 191 individuals for 1 probe/enzyme combination revealed one variant individual, which appeared to be heteroplasmic for two cpDNA haplotypes. It was not possible to determine whether this was due to biparental inheritance of somatic mutation within that individual. The implications of these results for further research on the structure and inheritance of cpDNA in gymnosperms, the use of cpDNA as a genetic market in P. sylvestris, and the conservation of Scottish populations of this species are discussed. Finally, it is suggested that an increased collaboration between molecular biologists and ecologists is the best way to approach studies using sophisticated molecular techniques to measure genetic diversity in natural populations. In this way, the potential of this approach to improve the management of genetic resources can be fully realised.
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Pharmawati, Made. "DNA-based approaches for development of markers to assist Grevillea and Leucadendron breeding." University of Western Australia. School of Plant Biology, 2006. http://theses.library.uwa.edu.au/adt-WU2006.0110.

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[Truncated abstract] Grevillea and Leucadendron belong to Proteaceae and both have economic importance to the floriculture industry. Grevillea is a highly diverse genus endemic to Australia and very attractive for landscaping. Leucadendron is a South African Proteaceae but is cultivated in Australia and is well known as a cut flower. This thesis focuses on the application of DNA-based molecular markers to these genera. Several groupings within Grevillea were suggested by previous researchers based on morphological characteristics. In this thesis the monophyly of the groupings among 12 Grevillea species from New South Wales was tested using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses. To test the robustness of the data, UPGMA using Jaccard similarity, Neighbor Joining using total character difference and Wagner parsimony analyses were undertaken. The relationship trees generated supported monophyly of the groupings. Chloroplast DNA (cpDNA) was used to develop phylogenetic relationships among Leucadendron species. Inheritance and variation of cpDNA were evaluated using PCR-RFLP. The study demonstrated that cpDNA was inherited maternally and a phylogenetic tree of Leucadendron species using parsimony analysis was constructed. ... A fingerprinting study conducted using ISSR, produced a dendrogram showing the relationships among 30 cultivars. From the results, i a fingerprinting key was developed. Three examples of synonymous cultivar pairs were identified. In Leucadendron the male and female flowers develop on separate plants, and sex identification is only possible at time of flowering. ISSR, suppression subtractive hybridisation (SSH), and SSH combined with mirror orientation selection (MOS) were used in attempts of identifying sex-dependent DNA fragments at earlier stages of plant development. Neither of these techniques was able to identify sex-specific markers in Leucadendron. Nevertheless, the results did indicate that cpDNA copy number may differentiate male and female plants. Also, it was demonstrated that the genomes of male and female plants are quite homologous, which increases the difficulty in identifying sex-specific sequences. This thesis highlights the potential of DNA-based markers to determine species relationships in Grevillea and Leucadendron, as well as to identify Leucadendron cultivars. The information produced during the research for this thesis provides a basis for Grevillea and Leucadendron variety development and may be used to assist the design of interspecific crosses, to identify cultivars and the parents of hybrids. In addition, the results offer insights into the likelihood, problems and strategies of finding sex-specific markers for genes controlling sex in Leucadendron. ii
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Stenson, Andrew. "Use of molecular markers at different taxonomic levels : evolution of the northern lesser Antillean anole radiation." Thesis, Bangor University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.327466.

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Drager, Robert Gray. "Molecular cloning of spinach chloroplast DNA isolated by alkaline lysis." PDXScholar, 1987. https://pdxscholar.library.pdx.edu/open_access_etds/3747.

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Chloroplast genomes of land plants show conservation of structure and gene arrangement. The spinach chloroplast genome is comprised of a covalently closed. circular DNA molecule of 150 kilobases and is typical of these plants. Approximately 20% of the proteins found in the spinach chloroplast are encoded by the chloroplast genome and translated on chloroplast ribosomes. The remainder are encoded on chromosomes in the nucleus, translated on cytoplasmic ribosomes and transported into the chloroplast. Spinach chloroplast DNA was isolated from crude 2 chloroplast preparations by a new method. Chloroplasts were lysed with alkaline sodium dodecyl sulfate, contaminating macromolecules precipitated with acidified potassium acetate and plastid DNA was purified by phenol:chloroform extraction and ethanol:ammonium acetate precipitation. The yield was approximately 50 ug chloroplast DNA per 100 grams leaf material. The DNA consisted of 10% circular molecules and 90% linear molecules. The chloroplast DNA was digested with restriction enzyme PstI and the fragments were cloned into the plasmid vector pUC9. Several recombinant plasmids were isolated and the chloroplast DNA inserts identified. The recombinant plasmid pRD105 containing the PstI #5 fragment was subjected to further investigation. The ClaI restriction sites of the PstI #5 fragment were mapped and the insert was subcloned into the plasmid vector pGEM4, which bears bacteriophage SP6 and T7 RNA polymerase promoter sequences.
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Vasemägi, Anti. "Evolutionary genetics of Atlantic salmon (Salmo salar L.) : molecular markers and applications /." Umeå : Dept. of Aquaculture, Swedish Univ. of Agricultural Sciences, 2004. http://epsilon.slu.se/s324.pdf.

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Fotinos, Tonya D. "Genetic Structure of the Florida Key Tree Cactus, Pilosocereus robinii, using Restriction Site associated DNA (RAD) markers." FIU Digital Commons, 2013. http://digitalcommons.fiu.edu/etd/914.

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Rare plant conservation efforts must utilize current genetic methods to ensure the evolutionary potential of populations is preserved. One such effort involves the Key Tree Cactus, Pilosocereus robinii, which is an endangered columnar cactus native to the Florida Keys. The populations have precipitously declined over the past decade because of habitat loss and increasing soil salinity from rising sea levels and storm surge. Next-generation DNA sequencing was used to assess the genetic structure of the populations. Twenty individuals representative of both wild and extirpated cacti were chosen for Restriction Site Associated DNA (RAD) analysis. Samples processed using the HindIII and NotIII restriction enzymes produced 82,382,440 high quality reads used for genetic mapping, from which 5,265 Single Nucleotide Polymorphisms (SNPs) were discovered. The analysis revealed that the Keys’ populations are closely related with little population differentiation. In addition, the populations display evidence of inbreeding and low genetic diversity.
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Books on the topic "Molecular markers; Plant genetics; DNA"

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Henry, Robert J. Molecular markers in plant improvement. Hoboken, N.J: John Wiley & Sons, 2013.

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Wisconsin-Madison), International Symposium on Plant Transposable Elements (1987 University of. Plant transposable elements. New York: Plenum Press, 1988.

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Sozinov, A. A. Molekuli︠a︡rno-geneticheskie markery i selekt︠s︡ii︠a︡ rasteniĭ: Materialy konferent︠s︡ii, Kiev, 10-13 mai︠a︡ 1994 g. Kiev: "Agrarna nauka", 1994.

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Boronnikova, S. V. Molekuli︠a︡rno-geneticheskai︠a︡ identifikat︠s︡ii︠a︡ i pasportizat︠s︡ii︠a︡ redkikh i nakhodi︠a︡shchikhsi︠a︡ pod ugrozoĭ ischeznovenii︠a︡ vidov rasteniĭ. Permʹ: Permskiĭ gos. universitet, 2009.

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Crop breeding: Methods and protocols. New York: Humana Press, 2014.

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Vasil, Indra K., and R. L. Phillips. DNA-based markers in plants. Springer, 2012.

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Phillips, R. L. Dna-Based Markers in Plants. Springer, 2010.

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L, Phillips Ronald, and Vasil I. K, eds. DNA-based markers in plants. 2nd ed. Dordrecht: Kluwer Academic Publishers, 2001.

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L, Phillips Ronald, and Vasil I. K, eds. DNA-based markers in plants. Dordrecht: Kluwer Academic, 1994.

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DNA-Based Markers in Plants (Advances in Cellular and Molecular Biology of Plants). 2nd ed. Springer, 2001.

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Book chapters on the topic "Molecular markers; Plant genetics; DNA"

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Young, Nevin Dale. "Constructing a plant genetic linkage map with DNA markers." In Advances in Cellular and Molecular Biology of Plants, 39–57. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-1104-1_3.

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Young, Nevin Dale. "Constructing a plant genetic linkage map with DNA markers." In Advances in Cellular and Molecular Biology of Plants, 31–47. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-015-9815-6_3.

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Koebner, Robert M. D., Wayne Powell, and Paolo Donini. "Contributions of DNA Molecular Marker Technologies to the Genetics and Breeding of Wheat and Barley." In Plant Breeding Reviews, 181–220. Oxford, UK: John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470650196.ch5.

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Rafalski, Antoni, Scott Tingey, and John G. K. Williams. "Random amplified polymorphic DNA (RAPD) markers." In Plant Molecular Biology, 55–63. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-017-6951-8_3.

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Rafalski, Antoni, Scott Tingey, and John G. K. Williams. "Random amplified polymorphic DNA (RAPD) markers." In Plant Molecular Biology Manual, 423–29. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0511-8_27.

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Hahn, William J., and Francesca T. Grifo. "Molecular Markers in Plant Conservation Genetics." In The Impact of Plant Molecular Genetics, 113–36. Boston, MA: Birkhäuser Boston, 1996. http://dx.doi.org/10.1007/978-1-4615-9855-8_7.

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Boopathi, N. Manikanda, and M. Raveendran. "Molecular Markers and DNA Barcoding in Moringa." In Compendium of Plant Genomes, 85–100. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-80956-0_9.

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Funke, Harald, Arnold von Eckardstein, and Gerd Assmann. "Molecular Genetics Approach to Polygenic Disease—Initial Results from Atherosclerosis Research." In DNA Polymorphisms as Disease Markers, 15–25. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-3690-1_3.

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O’Malley, David M. "Complex Trait Dissection in Forest Trees Using Molecular Markers." In The Impact of Plant Molecular Genetics, 49–70. Boston, MA: Birkhäuser Boston, 1996. http://dx.doi.org/10.1007/978-1-4615-9855-8_3.

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Bridges, William C., and B. W. S. Sobral. "Validation Strategies for Analysis of Quantitative Trait Loci Using Markers." In The Impact of Plant Molecular Genetics, 39–48. Boston, MA: Birkhäuser Boston, 1996. http://dx.doi.org/10.1007/978-1-4615-9855-8_2.

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Conference papers on the topic "Molecular markers; Plant genetics; DNA"

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"Transfer of rice resistance genes to blast using DNA markers." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-109.

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"Molecular analysis of BC1F1 and BC2F1 cotton hybrids using SSR markers." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-022.

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"Identification of DNA markers associated with starch granules morphology of Solanum tuberosum L." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-054.

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"Identification of the molecular markers linked to the chosen genes in cereals." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-091.

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"Development of DNA markers for the selection of spring barley varieties that are resistant to spot blotch." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-171.

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"Markers of somatic embryogenesis of Pinaceae species in in vitro culture (cytohistological, physiological and molecular genetic)." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-203.

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"Molecular-genetic analysis of DNA plasmotype of rye-wheat secalotriticum amphidiploids (RRAABB, 2n = 42)." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-109.

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"Breeding of potato resistant to late blight using genetic resources and DNA markers." In Current Challenges in Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences Novosibirsk State University, 2019. http://dx.doi.org/10.18699/icg-plantgen2019-69.

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"Identification of the stem rust resistance genes in the introgression lines of spring bread wheat using molecular markers." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-019.

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"Molecular markers of the SKr gene in the evaluation of bread wheat genotypes with different crossability with rye." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-160.

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