Relevant bibliographies by topics / Microsporea

Academic literature on the topic 'Microsporea'

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Published: 26 July 2024

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Journal articles on the topic "Microsporea"

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Devi Bunga Pagalla. "Stages of Microspore Development in Eggplant (Solanum melongena L.)." BIOEDUSCIENCE 7, no. 1 (April 30, 2023): 68–72. http://dx.doi.org/10.22236/jbes/7111357.

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Background: Microspores are small haploid spores that develop into the male gametophyte. Microsporocytes undergo meiotic division to form microspores. Microspores can be found in seedless and seed plants. The microspores in each flowering plant are different. This study aims to observe microspores on eggplant flowers. Method: Microspore observations were carried out on different flower bud sizes until the flower buds bloomed. Result: The results showed microspores in eggplant had different stages of development for each flower bud size. The stages of microspore development observed were Early uninucleate (Young microspore), Mid-uninucleate, Late uninucleate (Vacuolate microspore), early binucleate (Young bicellular pollen), mid-binucleate, and mature pollen. Conclusion: In eggplant microspore culture, anther length is a strong parameter to predict the stage of microspore development contained there in.
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Weidner, E., and A. Findley. "Extracellular Survival of an Intracellular Parasite (Spraguea lophii, Microsporea)." Biological Bulletin 197, no. 2 (October 1999): 270–71. http://dx.doi.org/10.2307/1542645.

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Kisera, Y. V., Y. V. Martyniv, and B. V. Gutyj. "Dynamics of morphological, immunological and histological changes in microsporіа in guinea pigs." Regulatory Mechanisms in Biosystems 12, no. 2 (May 22, 2021): 206–11. http://dx.doi.org/10.15421/022129.

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Microsporіа affect different species of animals and humans. The high contagiousness of the pathogen determines the relevance of research into this disease. Microsporum canis is the pathogen that most often causes microsporia. Weakened functions of the immune system and violation of the epithelial barrier of the skin are a favourable factor that causes microspores. The main source of infection is cats, which are involved in the storage and transmission of the pathogen. To clarify the dynamics of morphological, immunological and histological changes in microsporia, blood and skin studies of guinea pigs infected with M. canis were carried out. The animals were divided into two groups of 6 guinea pigs (healthy and sick). Test material (blood and skin) was taken from clinically healthy and sick animals 21 and 42 days after infection. The number of erythrocytes and leukocytes was determined by counting them in the Goryaev chamber, the hemoglobin content – by the method of cyanide hemoglobin. The leukogram was derived based on the counting and differentiation of 200 leukocyte cells in blood smears. Material for histological examination (pieces of skin) was fixed in 10–12% cooled solution of neutral formalin, followed by pouring in paraffin according to the scheme proposed by G. A. Merkulov. The obtained results demonstrated that leukocytosis developed in guinea pigs with microsporia on the 21st and 42nd days; the number of rod-shaped neutrophils increased, that of segmental neutrophils decreased, and that of ESR increased. The immune response to the course of microsporia was manifested in an increase in the percentage of T-lymphocytes, T-suppressors and a decrease in T-helper cells and an increase in T-killers compared with healthy animals. Histological examination showed that on the 21st day after infection, hyphae and spores of the fungus M. canis were localized in the skin. There is swelling of the dermis, stratification of collagen fibers and the accumulation of inflammatory infiltrates around the hair follicles. On the 42nd day, the infiltration spread and dystrophic changes in the skin occurred in the form of desquamation of the epidermis and the formation of acanthosis and hyperkeratosis on the surface of the dermis. The conducted research will allow further assessment of the course of microsporia under the action of various drugs and help establish the most effective method of treatment.
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Shaw, R. W., M. L. Kent, M. F. Docker, A. M. V. Brown, R. H. Devlin, and M. L. Adamson. "A New Species of Loma (Microsporea) in Shiner Perch (Cymatogaster aggregata)." Journal of Parasitology 83, no. 2 (April 1997): 296. http://dx.doi.org/10.2307/3284459.

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Pagalla, Devi Bunga, Ari Indrianto, Maryani Maryani, and Endang Semiarti. "Induction of Microspore Embryogenesis of Eggplant (Solanum melongena L.) ‘Gelatik’." Journal of Tropical Biodiversity and Biotechnology 5, no. 2 (August 15, 2020): 124. http://dx.doi.org/10.22146/jtbb.53677.

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The haploid or double haploid plant of eggplants could be produced from microspore culture (embryogenesis of microspores). In the breeding programs, microspore can be developed into an embryo directly after exposure to stress treatment during cultured. Stress (temperature and starvation medium) is an important factor in the induction of embryogenesis microspore. This study aims to induced embryogenic microspores from eggplant CV. Gelatik. The stage late-uninucleate microspore (Vacuolate Microspore/VM) and early binucleate (Young Bicellular Pollen/YBP) are the suitable stages to induce multinucleate structure. There are 3 methods used in this research; 1) Determination of the stage development of microspore based on flower buds length and anther length. 2) Induction of embryogenic microspore on the pre-treatment and starvation medium. 3) After giving pre-treatment for 4 days, micropores were transferred to culture medium A2 at 28oC in dark conditions to induce the multicellular structures. This study reported that 50-68.51% of the VM+YBP stage obtained in the range of flower bud lengths of 10-17 mm, and 5.0-6.9 mm, the range of anther length containing VM+YBP of 50-77.48%. The pre-treatment heat shock at 33oC in the medium B for 2 days, produced embryogenic microspores with a high percentage, that is about 50.19%, while microspores at 25oC and 4oC respectively 46.17% and 49.28%. Pre-treatment for 4 days at 4 oC, 25 oC, and 33oC with the percentage of embryogenic microspores apiece 32.87%, 27.45%, and 37.34%. The multicellular (starlike) structure begins forming on the fifth day of incubation in culture medium (A2) after pre-treatment in B medium at 33oC.
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Lavrushko, S. I., and V. I. Stepanenko. "Modern diagnostics of microsporia." Ukrainian Journal of Dermatology, Venerology, Cosmetology, no. 2 (June 29, 2021): 16–24. http://dx.doi.org/10.30978/ujdvk2021-2-16.

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Objective — to develop a method of modern molecular genetic diagnosis of microsporia in children based on polymerase chain reaction (PCR), which will allow identification of the pathogen of Microsporum canis at the DNA level. Materials and methods. The study included 40 patients with microsporia of smooth skin, scalp, scalp and smooth skin. The biological materials for the research were scales from the smooth skin and scalp, hair from the scalp of patients with microsporia. A study of 40 samples of biological material was carried out in patients with microsporia of smooth skin, microsporia of the scalp, microsporia of the scalp and smooth skin. At the first stage, DNA isolation of Microsporum canis was carried out. Then PCR was carried out to increase the copies of the DNA region using specific primers. The final step was typing 40 samples of clinical material of patients. Results and discussion. PCR diagnostics made it possible to identify the DNA of Microsporum canis in all 40 samples of biological material of patients with microsporia. In our study, we developed a PCR-based method for diagnosing microsporia, which uses a set of two MC primers (regions of the beta tubulin gene of Microsporum canis). For internal control of the course of amplification and the quality of biomaterial sampling, specific primers of APOE (a region of the human apolipoprotein E gene) were also used. Conclusions. In order to improve the precise specific diagnosis of microsporia in children, a method of modern molecular genetic diagnostics based on polymerase chain reaction (PCR) has been developed, which allows identification of the Microsporum canis pathogen at the DNA level. Analysis of the molecular structure of the genome of Microsporum canis proved that the most objective diagnosis of microorganisms is the PCR method. The developed method of DNA diagnostics based on PCR using specific primers can be included in the algorithm for detecting Microsporum canis in humans.
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Desser, Sherwin S., and John R. Barta. "Nosema jirivavrai n.sp. (Microsporea; Protozoa) from the leech Batracobdella picta in Ontario." Canadian Journal of Zoology 67, no. 11 (November 1, 1989): 2640–45. http://dx.doi.org/10.1139/z89-373.

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A new microsporidian species was described from the muscle and connective tissue of the glossophoniid leech Batracobdella picta. Subepithelial xenomas contained sporonts, sporoblasts, and spores. Merogonic stages were not observed. All sporogonic stages were diplokaryotic. Mature spores were ovoid, 3.0–3.6 × 1.8–2.2 μm. The spore wall was 190–240 nm thick, with a distinct exospore, endospore, and underlying plasmalemma. The polar filament had 12–13 coils and measured 105 nm in diameter. The angle of tilt of the anterior coils of the filament to the vertical axis of the spore was about 63°. This newly discovered parasite was named Nosema jirivavrai.
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Zhao, Z. Y., and D. F. Weber. "Analysis of Nondisjunction Induced by the R-X1 Deficiency during Microsporogenesis in Zea Mays L." Genetics 119, no. 4 (August 1, 1988): 975–80. http://dx.doi.org/10.1093/genetics/119.4.975.

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Abstract The r-X1 deficiency in maize induces nondisjunction at the second mitotic division during embryo sac formation. However, it was not known if this deficiency also induces nondisjunction during the microspore divisions. Microsporogenesis in plants lacking or containing this deficiency was compared using two approaches. First, chromosome numbers were determined in generative nuclei. Many (8.3%) of the generative nuclei in r-X1-containing plants were aneuploid; however, those from control plants were all haploid. Thus, this deficiency induces nondisjunction during the first microspore division. Second, nucleoli were analyzed in microspores. The only nucleolar organizing region in maize is on chromosome 6. If chromosome 6 underwent nondisjunction during the first microspore division, one nucleus in binucleate microspores would contain no nucleolus and the other would contain two nucleoli (or one nucleolus if the nucleoli fused). Only one (0.03%) microspore of this type was observed in control plants while 1.12% were found in r-X1-containing plants. Thus, the r-X1 deficiency induces nondisjunction of chromosome 6 during the first microspore division. However, both of the sperm nuclei in trinucleate microspores contained one nucleolus in r-X1-containing and control plants; thus, this deficiency does not induce nondisjunction of chromosome 6 (and presumably other chromosomes) during the second microspore division.
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Martyniv, Yu V., and Ia V. Kisera. "Changes of hematological parameters of in blood in cats ill with microsporium." Scientific Messenger of LNU of Veterinary Medicine and Biotechnology 21, no. 93 (April 2, 2019): 70–73. http://dx.doi.org/10.32718/nvlvet9313.

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There has been a massive tendency for cats to be kept as pets in Ukraine in recent years. The frequency of their diseases has also increased at the same time. Cats most often come into the homes of people from the street, from volunteers, rarely from nurseries. Due to this, Doctors often receive cats ill for microsporia, which is caused by fungi of the genus Microsporum and is one of the most common anthropozoonous diseases. The treatment process is carried out by a complex method. Analysis of the recommendations of various authors on the treatment of microsporia indicates the lack of immunostimulants in the conduct of a complex of therapeutic and preventive measures. Hematological studies were performed in order to find out the immune reactivity of the cats' organism during microsporia. The research was conducted on clinically healthy and cats ill for microspores. The obtained results of research showed that in cats with microsporia changes in morphological composition of blood were characterized by signs of anemia, leukopenia and lymphocytopenia. Changes in the structure of neutrophils were found in the type of vacuum and toxigenic grains in cats ill for microsporia. The toxic grains of neutrophils occur inside the cell as a result of physicochemical changes in the protein structure of the cytoplasm. Such cells can not provide phagocytosis of foreign agents and thus reduce the immune activity of the organism in cats ill for microsporium. A marked change in the shape of erythrocytes, which is characteristic of anemia, that is, erythrocytes-octantocytes with corneas by Jolly inclusions. Jolly's bodies are the remnants of the nucleus that have survived in erythrocytes because of the broken destruction of the normoblast nucleus. The obtained results indicate that the course and manifestation of microsporia in cats affects the immune status of the organism.
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Nguyen, Minh Ly, and Ton Nu Bao Tien Huyen. "Effects of culture conditions on isolated microspore culture of melon (Cucumis melo L.)." Ministry of Science and Technology, Vietnam 65, no. 2 (June 15, 2023): 30–36. http://dx.doi.org/10.31276/vjste.65(2).30-36.

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Isolated microspore culture is a useful tool to produce pure, fully homozygous parental lines in a short time. This study evaluated factors including the microspore developmental stage, cell culture density, and heat treatment influencing callus formation from melon microspores (Cucumis melo L.). The results showed that the obtained number of calli induced was highest (11.00±1.02) when cultivating flower buds with sizes 7.0-7.9 mm that contained microspores at middle-to-late uninucleate stages. The optimal microspore density for culture is4×104 cells. The cultured medium was NLN, containing 130 g/l of sucrose at pH 5.8. Heat treatment at 40ºC for 48 hours was best suited for callus induction of all flower bud sizes. The survival rate of microspores after 7 days of culture was lower than before inoculation and was only 75.6%. The development of the microspores and the arising of calli and embryos have been observed and evaluated for morphological cell characteristics. However, in this study, no mature embryo formation or seedling regeneration was observed.
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Dissertations / Theses on the topic "Microsporea"

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Sato, Vanessa Sayuri [UNESP]. "Produção de fitase por Rhizopus microsporus var. microsporus: purificação, caracterização bioquímica e aplicação." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/124519.

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A investigação biotecnológica acompanhada da aplicação das enzimas, combinada com o uso da engenharia genética tem sido realizada em micro-organismos para a produção de enzimas para fins industriais. Entre estas enzimas, as fitases microbianas, que catalisam a hidrólise do fitato (mio-inositol hexaquisfosfato) a mio-inositol e fosfato inorgânico, têm sido amplamente utilizadas na alimentação animal. Neste contexto, o fungo R. microsporus var. microsporus foi selecionado como bom produtor de fitases, com maiores níveis de produção encontrados na Fermentação por Biofilmes (FB) (261,30 U/mg), utilizando bagaço de cana de açúcar como fonte de carbono adicional. A morfologia do biofilme sobre suporte inerte foi analisada por MEV observando-se múltiplas hifas interligadas formando um conjunto ordenado na presença de inúmeros canais que permitem a troca eficiente de nutrientes e oxigenação. A fitase extracelular obtida foi purificada 4,18 vezes com recuperação de 4,78%, obtendo-se uma única banda de 34 kDa em SDS PAGE 12%. A temperatura ótima de atividade para a enzima purificada foi de 55ºC e o pH ótimo 4,5, sendo estável entre 30 °C e 40 °C por 120 min. Apresentou baixa estabilidade ao pH com atividade residual acima de 50% entre pH 3,0 e 5,0 por 60 min. A fitase foi ativada por íons Ca2+ e inibida por K+. A enzima foi capaz de hidrolisar fitato de sódio (Km de 0,72 mM e Vmax de 94,55 U/mg). O extrato bruto contendo fitase foi seco em Spray Dryer com diferentes adjuvantes (farelo de milho, farelo de soja, fubá, amido e maltodextrina). O farelo de soja possibilitou a maior recuperação da atividade fitásica (60%), assim como o fubá (59,5%). Considerando o uso destes dois adjuvantes, o pH ótimo de atividade para a fitase contida no extrato seco foi 4,5 e 8,5, respectivamente e a temperatura ótima de atividade de 45-50 °C. Quando utilizado fubá, a estabilidade foi mantida...
Biotechnological research, and the application of enzymes in combination with the use of genetic engineering has been carried out in microorganisms for the production of enzymes for industrial purposes. Among these enzymes, microbial phytases, which catalyze the hydrolysis of phytate (myo-inositol hexakisphosphate) to myo-inositol and inorganic phosphate, have been widely used in animal feed. In this context, the fungus R. microsporus var. microsporus was selected as a good producer of the phytase with higher production levels when grown on Biofilm Fermentation (FB) (261,30 U/mg), using sugarcane bagasse as carbon additional source. The morphology of biofilms on inert support was examined by SEM observing interconnected hyphae forming an ordered array in the presence of many channels which enables efficient exchange of nutrients and oxygen. The extracellular phytase was purified 4.18 fold with 4.78% recovery. A single protein band was obtained in 6% PAGE and confirmed by 12% SDS-PAGE as a single protein band with 34 kDa. The optimum temperature for activity was 55 °C and the optimum pH 4.5. It was completely stable at temperatures between 30 °C and 40 °C for 120 min had low pH with a residual activity of over 50% between pH 3.0 and 5.0 for 60 min. The enzyme was activated by Ca2+ and inhibited by Zn2+. The enzyme was able to hydrolyze sodium phytate with a Km=0.72 mM, Vmax= 94.55 U/mg of protein. The crude extract containing phytase was dried using Spray Dryer with different adjuvants. Soybean meal and corn meal allowed the best recovery of phytase activity 60% and 59.5%, respectively. Considering the use of these two adjuvants, the optimum pH of activity for phytase in the dry extract was 4.5 and 8.5, respectively, and the optimum of temperature of 45-50 °C. When used corn meal, the stability was maintained above 90% at pH 2.5-10.0 for 60 min. The dry phytase (corn meal) was applied to the feed...
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Sato, Vanessa Sayuri. "Produção de fitase por Rhizopus microsporus var. microsporus : purificação, caracterização bioquímica e aplicação /." Araraquara, 2015. http://hdl.handle.net/11449/124519.

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Orientador: Luis Henrique Souza Guimarães
Banca: Daniela Alonso Bocchini Martins
Banca: José Roberto Ernandes
Banca: João Cláudio Thoméo
Banca: Patricia Gomes Cardoso
Resumo: A investigação biotecnológica acompanhada da aplicação das enzimas, combinada com o uso da engenharia genética tem sido realizada em micro-organismos para a produção de enzimas para fins industriais. Entre estas enzimas, as fitases microbianas, que catalisam a hidrólise do fitato (mio-inositol hexaquisfosfato) a mio-inositol e fosfato inorgânico, têm sido amplamente utilizadas na alimentação animal. Neste contexto, o fungo R. microsporus var. microsporus foi selecionado como bom produtor de fitases, com maiores níveis de produção encontrados na Fermentação por Biofilmes (FB) (261,30 U/mg), utilizando bagaço de cana de açúcar como fonte de carbono adicional. A morfologia do biofilme sobre suporte inerte foi analisada por MEV observando-se múltiplas hifas interligadas formando um conjunto ordenado na presença de inúmeros canais que permitem a troca eficiente de nutrientes e oxigenação. A fitase extracelular obtida foi purificada 4,18 vezes com recuperação de 4,78%, obtendo-se uma única banda de 34 kDa em SDS PAGE 12%. A temperatura ótima de atividade para a enzima purificada foi de 55ºC e o pH ótimo 4,5, sendo estável entre 30 °C e 40 °C por 120 min. Apresentou baixa estabilidade ao pH com atividade residual acima de 50% entre pH 3,0 e 5,0 por 60 min. A fitase foi ativada por íons Ca2+ e inibida por K+. A enzima foi capaz de hidrolisar fitato de sódio (Km de 0,72 mM e Vmax de 94,55 U/mg). O extrato bruto contendo fitase foi seco em Spray Dryer com diferentes adjuvantes (farelo de milho, farelo de soja, fubá, amido e maltodextrina). O farelo de soja possibilitou a maior recuperação da atividade fitásica (60%), assim como o fubá (59,5%). Considerando o uso destes dois adjuvantes, o pH ótimo de atividade para a fitase contida no extrato seco foi 4,5 e 8,5, respectivamente e a temperatura ótima de atividade de 45-50 °C. Quando utilizado fubá, a estabilidade foi mantida...
Abstract: Biotechnological research, and the application of enzymes in combination with the use of genetic engineering has been carried out in microorganisms for the production of enzymes for industrial purposes. Among these enzymes, microbial phytases, which catalyze the hydrolysis of phytate (myo-inositol hexakisphosphate) to myo-inositol and inorganic phosphate, have been widely used in animal feed. In this context, the fungus R. microsporus var. microsporus was selected as a good producer of the phytase with higher production levels when grown on Biofilm Fermentation (FB) (261,30 U/mg), using sugarcane bagasse as carbon additional source. The morphology of biofilms on inert support was examined by SEM observing interconnected hyphae forming an ordered array in the presence of many channels which enables efficient exchange of nutrients and oxygen. The extracellular phytase was purified 4.18 fold with 4.78% recovery. A single protein band was obtained in 6% PAGE and confirmed by 12% SDS-PAGE as a single protein band with 34 kDa. The optimum temperature for activity was 55 °C and the optimum pH 4.5. It was completely stable at temperatures between 30 °C and 40 °C for 120 min had low pH with a residual activity of over 50% between pH 3.0 and 5.0 for 60 min. The enzyme was activated by Ca2+ and inhibited by Zn2+. The enzyme was able to hydrolyze sodium phytate with a Km=0.72 mM, Vmax= 94.55 U/mg of protein. The crude extract containing phytase was dried using Spray Dryer with different adjuvants. Soybean meal and corn meal allowed the best recovery of phytase activity 60% and 59.5%, respectively. Considering the use of these two adjuvants, the optimum pH of activity for phytase in the dry extract was 4.5 and 8.5, respectively, and the optimum of temperature of 45-50 °C. When used corn meal, the stability was maintained above 90% at pH 2.5-10.0 for 60 min. The dry phytase (corn meal) was applied to the feed...
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Липовська, Вікторія Вікторівна, Виктория Викторовна Липовская, Viktoriia Viktorivna Lypovska, and М. О. Крамар. "Епідеміологічна ситуація по захворюваності дітей на мікроспорію у північно-східному регіоні України." Thesis, Сумський державний університет, 2015. http://essuir.sumdu.edu.ua/handle/123456789/41994.

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Мікроспорія відноситься до найбільш поширених захворювань мікотичної етіології у педіатричній практиці, займаючи друге місце за розповсюдженістю в світі та Україні після мікозів стоп. Мікоз характеризується високою контагіозністю та швидким поширенням. Для запобігання розповсюдження захворювання серед дітей необхідно володіння лікарями інформацією про епідеміологічну ситуацію щодо захворюваності на мікотичні інфекції, зокрема на мікроспорію.
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Морозова, О. О., and В. Е. Коваленко. "Порівняльна характеристика захворювання на мікроспорію у деяких країнах світу та в Україні." Thesis, Сумський державний університет, 2015. http://essuir.sumdu.edu.ua/handle/123456789/42211.

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У сучасній дерматологічній практиці грибкові захворювання посідають одне з провідних місць, не поступаючись за кількістю жодному дерматозу. За різними оцінками, на частку мікозів припадає від 37 до 42% від усіх хвороб шкіри. Найбільш актуальною серед грибкових хвороб є мікроспорія, яка зустрічається на всіх континентах (за вийнятком Антарктиди).
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Guyon, Virginie Noelle Veronique. "Molecular study of microspore development in Brassica napus." Thesis, University of Reading, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387037.

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Naktinskaitė, Lina. "Dermatofito microsporum canis jautrumas dezinfekcinėms medžiagoms." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140305_140350-34848.

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Lietuvoje registruotų dezinfekcinių priemonių: baliklio, TH4+, Safe 4, Ecocid S, formalino, buvo atliktas tyrimas, nustatyti Microsporum canis jautrumą, dezinfektantui. Patogeninio mikromiceto M. canis kolonijos išskirtos iš dermatofitija sergančių kačių.
Experiment was done to determine Microsporum canis sensitivity for disinfectant, using detergents which are registered in Lithuania: bleach , TH4 + , Safe 4 , S ecocide , formalin. Micromycetes pathogenic M. canis colonies isolated from cats which infected by dermatophytosis.
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Hunter, Clifford Paul. "Plant regeneration from microspores of barley Hordeum vulgare L." Thesis, Imperial College London, 1988. http://hdl.handle.net/10044/1/7765.

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Scott, Peter. "The metabolism of sucrose and maltose by barley microspores." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.239199.

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Ornela, Pedro Henrique de Oliveira. "Co-purificação e caracterização das fosfatase e fitase alcalinas de Rhizopus microsporus var. microsporus produzidas em fermentação submersa /." Araraquara, 2017. http://hdl.handle.net/11449/151602.

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Orientador: Luis Henrique Souza Guimarães
Banca: Ariela Veloso de Paula
Banca: Hamilton Cabral
Resumo: A investigação biotecnológica, acompanhada da aplicação das enzimas, tem sido realizada em microrganismos para a produção de enzimas para fins industriais. Entre estas enzimas, as fosfatases, responsáveis por hidrolisar ésteres e anidridos de ácido fosfórico, e as fitases microbianas, que catalisam a hidrólise do fitato (mio-inositol hexaquisfosfato) em mio-inositol e fosfato inorgânico, têm sido amplamente utilizadas em diferentes setores como, por exemplo, em experimentos de biologia molecular e na alimentação animal. De acordo com o pH ótimo de reação, as fosfatases são divididas em alcalinas (EC 3.1.3.1) e ácidas (EC 3.1.3.2). As fitases são enzimas que também pertencem à classe das fosfatases, hidrolisando, no entanto, de forma específica, o ácido fítico. Em recentes trabalhos, o fungo filamentoso Rhizopus microsporus var. microsporus apresentou potencialidade na produção de fosfatases e fitases. Diante disto, este estudo visou a produção, a purificação e caracterização da fosfatase e da fitase alcalina produzidas por R. microsporus var. microsporus. No processo de otimização em Fermentação Submersa (FSbm), a maior produção enzimática foi em meio Khanna com 0,4 mM de KH2PO3 e adicionado de 0,5% de farinha de centeio por 76 h, 32ºC, pH 6,3, a 100 rpm. Em colunas cromatográficas, a fosfatase alcalina foi purificada 10 vezes e com recuperação de 13%, e a fitase alcalina foi purificada 86 vezes com recuperação de 167%. A massa molecular nativa da fosfatase e da fitase alcali... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Biotechnological research, accompanied by the application of enzymes, has been carried out in microorganisms for production of enzymes for industrial purposes. Among these enzymes, microbial phosphatases, responsible for hydrolyzing phosphoric acid esters and anhydrides, and phytases, which catalyzes the hydrolysis of phytate (myo-inositol hexaquisphosphate) in myo-inositol and inorganic phosphate, have been widely used in different sectors as, for example, in molecular biology experiments and in animal feed. According to the optimum reaction pH, phosphatases are divided into alkaline (EC 3.1.3.1) and acidic (EC 3.1.3.2). Phytases are enzymes that also belong to the class of phosphatases, however, hydrolyzing phytic acid. In recent works, the filamentous fungus Rhizopus microsporus var. microsporus presented potential for production of phosphatases and phytases. In view of this, this study aimed at the production, purification and characterization of phosphatase and alkaline phytase produced by R. microsporus var. microsporus. In the optimization of Submerged Fermentation (FSbm), the highest enzymatic production was in Khanna medium with 0.4 mM KH2PO3 and added with 0.5% rye flour for 76 h, 32ºC, pH 6.3, at 100 rpm. In chromatographic columns, alkaline phosphatase was purified 10 folds and recovered at 13%, and alkaline phytase was purified 86 folds with recovery of 167%. The native molecular mass of alkaline phosphatase and phytase produced by R. microsporus var. microsporus... (Complete abstract click electronic access below)
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Zhao, Jiping. "Induction and mechanism of Brassica napus cv. Topas microspore embryogenesis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq20600.pdf.

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Books on the topic "Microsporea"

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Murray, Wittner, and Weiss Louis M, eds. The microsporidia and microsporidiosis. Washington, D.C: ASM Press, 1999.

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1938-, Margulis Lynn, McKhann Heather I, and Olendzenski Lorraine, eds. Illustrated glossary of protoctista: Vocabulary of the algae, apicomplexa, ciliates, foraminifera, microspora, water molds, slime molds, and the other protoctists. Boston: Jones and Bartlett Publishers, 1993.

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Wilson, G. G. A comparison of the effects of Nosema fumiferanae and a Nosema sp. (microsporida) on Choristoneura fumiferana (Clem.) and Choristoneura pinus pinus Free. Sault Ste. Marie, Ont: Forest Pest Management Institute, 1986.

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Wilson, G. G. Observations on the level of infection and intensity of Nosema fumiferanae (microsporida) in two different field populations of the spruce budworm, Choristoneura fumiferana. Sault Ste. Marie, Ontario: Forest Pest Management Institute, 1987.

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Weiss, Louis M., and Murray Wittner. Microsporidia and Microsporidiosis. Wiley & Sons, Limited, John, 2014.

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Cryptosporidiosis and Microsporidiosis (Contributions to Microbiology). S. Karger AG (Switzerland), 2000.

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Stephen, Blackmore, and Knox R. Bruce, eds. Microspores: Revolution and ontogeny. London: Academic, 1990.

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Microspores Evolution and Ontogeny. Elsevier, 1990. http://dx.doi.org/10.1016/c2009-0-03229-2.

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Blackmore, S. Microspores: Evolution and Ontogeny. Academic Pr, 1991.

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Blackmore, S., and R. B. Knox. Microspores Evolution and Ontogeny: Evolution and Ontogeny. Elsevier Science & Technology Books, 2016.

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Book chapters on the topic "Microsporea"

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Olmedilla, A. "Microspore Embryogenesis." In Plant Developmental Biology - Biotechnological Perspectives, 27–44. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-04670-4_2.

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Tashpulatov, Alisher, Ari Indrianto, Ioulia Barinova, Heidrun Katholnigg, Svetlana Akimcheva, Erwin Heberle-Bors, and Alisher Touraev. "Microspore Embryogenesis." In Plant Biotechnology 2002 and Beyond, 529–35. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-2679-5_109.

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Dunwell, J. M. "Microspore culture." In In Vitro Haploid Production in Higher Plants, 205–16. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-017-1860-8_12.

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Dickinson, H. G. "Microspore Derived Embryogenesis." In Sexual Plant Reproduction, 1–15. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-77677-9_1.

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Kretschmar, Marianne. "Microsporum audouinii." In Lexikon der Infektionskrankheiten des Menschen, 519–21. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-39026-8_688.

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Kretschmar, Marianne. "Microsporum canis." In Lexikon der Infektionskrankheiten des Menschen, 521–24. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-39026-8_689.

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Kretschmar, Marianne. "Microsporum ferrugineum." In Lexikon der Infektionskrankheiten des Menschen, 524–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-39026-8_690.

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Kretschmar, Marianne. "Microsporum gypseum." In Lexikon der Infektionskrankheiten des Menschen, 525–27. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-39026-8_691.

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Kretschmar, Marianne. "Microsporum persicolor." In Lexikon der Infektionskrankheiten des Menschen, 528–29. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-39026-8_692.

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Pulli, S., and Y. D. Guo. "Microspore culture of rye." In Doubled Haploid Production in Crop Plants, 151–54. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-1293-4_23.

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Conference papers on the topic "Microsporea"

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Skogoreva, A. M., and E. S. Klimkina. "IMPROVING MICROSPORIA THERAPY IN CATS." In Современные проблемы общей и прикладной паразитологии. Воронеж: Цифровая полиграфия, 2022. http://dx.doi.org/10.57007/9785907283979_2022_16_169-173.

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Bobkov, S. V. "Reprogramming pea microspores on a sporophytic path of development." In IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future". Kazan University Press, 2019. http://dx.doi.org/10.26907/978-5-00130-204-9-2019-71.

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Jiean, Liao, Hu Can, Wang Xufeng, Wang Wei, and Liu Xinying. "Study on Ultrasonic Vibration Deep Microspore Drilling Bit Dynamic State." In 2014 Fifth International Conference on Intelligent Systems Design and Engineering Applications (ISDEA). IEEE, 2014. http://dx.doi.org/10.1109/isdea.2014.216.

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Galinari, Camila Barros, TIAGO DE PAULA BIANCHI, POLLYANNA CRISTINA VICENZI CONRADO, PATRÍCIA DE SOUZA BONFIM DE MENDONÇA, and TEREZINHA INEZ ESTIVALET SVIDZINSKI. "INTERNALIZAÇÃO DA HIPERICINA NANOENCAPSULADA NO FUNGO MICROSPORUM CANIS." In II Congresso Nacional de Microbiologia Clínica On-line. Revista Multidisciplinar em Saúde, 2022. http://dx.doi.org/10.51161/ii-conamic/26.

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Introdução: Infecções originadas por Microsporum canis são caracterizadas por recorrência e o fracasso no tratamento varia entre 25-40% dos pacientes tratados. Esta lacuna, promove a busca por novas alternativas terapêuticas como a Inativação Fotodinâmica (IFD). Neste contexto, vem se destacado a utilização da hipericina nanoencapsulada em P123 (Hip-P123), como fotossensibilizador (FS). A Hip-P123 quando excitada em um comprimento de onda específico, na presença de oxigênio, promove reações fotoquímicas, desenvolvendo danos celulares e inativação de microrganismos. Neste sentido, o tratamento com IFD e Hip-123 poderia ser uma alternativa para a inativação do M. canis. Objetivo: Avaliar a absorção intracelular da Hip-P123 em células planctônicas de M. canis, para posterior IFT. Materiais e Métodos: M. canis foi suspenso a concentração de 1.105 conídios/mL. Posteriormente, 100 µl de Hip-P123 (12,5; 6,25 e 3,125 µMol) foram adicionados aos poços e levado a incubação por 2h no escuro à 25°C. Após a incubação, a suspenção foi lavada 2x e ressuspensa em salina estéril. A ligação e internalização da Hip-P123 aos microconídios foi avaliada por citometro de fluxo (BD FACSCaliburTM), emitindo um espectro de excitação na faixa de 550-750 nm. Para detectar a fluorescência do FS foram contados 10.000 eventos. Microconídios somente com salina foram utilizados como controle não-tratado. Resultados: Na análise por citometria de fluxo, a intensidade de fluorescência está correlacionada a internalização do FS. Nossos resultados demonstram que intensidade de fluorescência apresentada pelos microconídios tratados com Hip-P123 foi maior quando comparada as células não tratadas. Sendo que o tratamento com 12,5 e 6,25 µMol de Hip-P123 apresentaram fluorescência similar, corroborando com os resultados preliminares de atividade antifúngica. Conclusão: Estes resultados demonstram a absorção intracelular da Hip-P123 no M. canis, indicando a atividade do FS durante a inativação fotodinâmica.
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Ali, Nashwan Mohammad, and Shaimaa Nabhan Yassein. "Isolation and identification of Microsporum canis from pet animals in Baghdad province." In PROCEEDING OF THE 1ST INTERNATIONAL CONFERENCE ON ADVANCED RESEARCH IN PURE AND APPLIED SCIENCE (ICARPAS2021): Third Annual Conference of Al-Muthanna University/College of Science. AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0106913.

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Santos, Edgleidson Silva dos, Valeria Bentes Ferreira, Nicollas Tomás De Aquino Motta, Fabricia Duarte Omena, Cintia Da Silva Luiz, and Rodrigo Antonio Torres Matos. "ETIOLOGIA DAS INFECÇÕES FÚNGICAS DE CÃES E GATOS." In I Congresso On-line Nacional de Clínica Veterinária de Pequenos Animais. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1864.

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Introdução: As infecções fúngicas ocorrem com grande frequência na rotina clínica de pequenos animais, devido a fatores como resposta imune do hospedeiro e ampla distribuição do agente no ambiente. Estas infecções são conhecidas como micoses, sendo classificadas como superficiais, subcutâneas e sistêmicas. Objetivo: Objetivou-se com presente trabalho identificar os fungos isolados de amostras clínicas de cães e gatos com infecções fúngicas. Material e métodos: No período de setembro/2020 a março/2021, realizou-se o levantamento das fichas e laudos dos animais relacionados aos casos de infecções fúngicas, obtidos no Centro de Diagnostico Veterinário (VETLAB). A partir das análises dos arquivos, obteve-se dados como raça, sexo e idade. Além disso, foi possível identificar os micro-organismos isolados por meio de amostras colhidas. Resultados: Foram avaliados 52 animais, sendo 57,6% (30/52) cães e 42,4% (22/52) felinos. Os cães possuíam idades entre três meses a 15 anos e os felinos de um mês a oito anos. Em relação aos cães os de maiores incidências no período foram das raças Yorkshire e SRD com 16,66% (5/30), Spitz e Pug com 13% ( 4/30), Pinscher e Chiuahua 6,6% ( 2/30) e os da raça Pastor Maremano, Bulldog Francês, Jack Russell Terrier, Branco, Maltês, Lulu da pomerânia, Dachshund e Bulldog Inglês com 3,33% (1/30) cada. Tiveram predominância nos felinos, SRD com 86,37% (19/22) e Persa com 13,63% (3/22). Destes 52 animais, foram colhidas amostras de pele, pelo e conduto auditivo. Houve maior ocorrência de Penicillium sp. 23,07% (12/52), Microsporum sp. 13,4% (7/52), Microsporum canis sp. 11,5% (6/52), Aspergillus niger 7,69% (4/52), Dermatófito e Malassezia sp. com 5,76% (3/52), Aspergillus sp. 3,84% (2/52), Trichophyton sp. 1,92% (1/52) e em 25% (13/52) das amostras não houve o crescimento fúngico. Conclusão: Diante dos resultados, observou-se que os fungos do gênero Penicillium sp. e Microsporum sp., foram mais prevalentes nas afecções fúngicas. Devido ao aumento das infeções, ressalta-se a importância da realização de cultura fúngicas e o antifungigrama, permitindo um melhor diagnóstico e adequação da alternativa terapêutica.
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COSTA, GRACIELE PEREIRA, DANIELLE PEREIRA COSTA SILVA, DIANA DE OLIVEIRA AZEVEDO CARVALHO ROCHA, HÉLEN LARISSA DA COSTA MENDES, and QUÉZIA AUANE SILVA DONATO. "MICROSPOROSE CANINA – UM RELATO DE CASO." In I Congresso Nacional de Especialidades Veterinárias On-line. Revista Multidisciplinar em Saúde, 2022. http://dx.doi.org/10.51161/convesp/6493.

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Introdução: As dermatofitoses estão distribuídas em todo o mundo, principalmente em ambientes tropicais e temperados. São causadas por várias espécies de dermatófitos, sendo classificadas pelos gêneros Microsporum, Trichophyton e Epidermophyton, sendo que o Microsporum canis é o mais comumente encontrado na clínica de pequenos animais. A microscoporose se trata de uma zoonose, podendo ser transmitido ao homem por animais domésticos. Objetivos: Objetivou-se com a realização deste estudo relatar o caso clínico de um cão com microsporose e analisar as alterações clínicas e laboratoriais encontradas. Metodologia: Trata-se de um relato de caso de um paciente canino com microsporose. Relato de caso: canino, não castrado, shih tzu, com 3 anos de idade, pesando 5,7 Kg, foi atendido em um hospital veterinário em Guanambi/ Bahia, apresentando coceira, feridas próximas ao saco escrotal, de cor avermelhada. Durante o atendimento foi realizada coleta sanguínea para hemograma completo, teste rápido de leishmaniose, raspado de pele e de pêlos para cultura de fungos. O exame de raspado de pele foi confeccionado com hidróxido de potássio e levado ao microscópio para análise, e a cultura foi realizada em meio, e aguardou-se para o resultado da mesma. Discussão: No exame de hemograma não houve alterações significativas, o teste rápido de leishmaniose foi negativo, no raspado de pele foi constatado presença de hifas, e a cultura foi positiva para dermatofitose, sendo confirmado presença de Microsporum canis através da técnica de coloração de gram. O diagnóstico foi então concluído de acordo com o histórico, anamnese e exame complementar. O tratamento instituído foi o itraconazol (10mg/kg, sid, durante 30 dias), hepvet (1 comp, sid, durante 50 dias), banhos com cetoconazol shampoo (durante 30 dias). O animal se manteve estável durante o tratamento, havendo melhora constante e se obtendo ao final do tratamento cura da enfermidade. Conclusão: O prognóstico para dermatofitoses é favorável quando realizado o tratamento de modo adequado, sendo de suma importância uma boa coleta de locais estratégicos por parte do veterinário para que não ocorra falsos negativos, e a realização de exame de microscopia direta e principalmente cultura fúngica para diagnóstico e consequentemente um tratamento de sucesso.
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Vishnyakova, A. V., and A. A. Alexandrova. "Study of the influence of various factors on the regenerative capacity of spring rape embryoids obtained in culture of microspores." In Agrobiotechnology-2021. Publishing house of RGAU - MSHA, 2021. http://dx.doi.org/10.26897/978-5-9675-1855-3-2021-135.

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The article presents the results of a research the effect of acidity of the environment, cold treatment and cultivation on a medium with half the content of macro- and microelements (1/2 B5) on the regeneration of spring rape embryoids.
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Prameswari, F., A. Oetari, and I. Santoso. "Growth of Rhizopus microsporus UICC 500, UICC 531, and UICC 539 on the palm oil processing waste." In PROCEEDINGS OF THE 3RD INTERNATIONAL SYMPOSIUM ON CURRENT PROGRESS IN MATHEMATICS AND SCIENCES 2017 (ISCPMS2017). Author(s), 2018. http://dx.doi.org/10.1063/1.5064144.

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Waleska Rodrigues de Araújo Cavalcanti, Alydyanny, Alicia Kelly Mucarbel dos Santos, Lucas Valeriano Marques, Karollainy Vasconcelos Cavalcanti, Renata de Barros Ferraz, and Raquel Desenzi Pessoa. "A dermatofitose na clínica médica de pequenos animais e sua característica zoonótica." In Congresso Online Acadêmico de Medicina Veterinária. Congresse.me, 2022. http://dx.doi.org/10.54265/hvmb8670.

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Introdução: Na rotina dermatológica de pequenos animais são recorrentes os casos de dermatofitose, que merecem uma maior atenção por se tratar de uma zoonose e também uma antropozoonose. A dermatofitose é uma infecção que ocorre na região superficial da pele, podendo acometer cães, gatos e outros animais domésticos. Essa infecção é causada por um grupo de fungos chamados de dermatófitos, no Brasil temos o Microsporum canis, Microsporum gypseum e Trichophyton mentagrophytes, contudo, os casos de maior incidência são causados pelo Microsporum canis. Esse grupo de fungos colonizam tecidos queratinizados como pelos e unhas, utilizando esta queratina como nutriente. Os casos mais frequentes ocorrem em filhotes e esse fator pode estar relacionado com a saúde do animal e com o local onde ele vive. A transmissão ocorre por contato direto com outro animal infectado, humanos, fômites ou por esporos fúngicos que ficam no ambiente. No diagnóstico, a anamnese e o exame físico são de suma importância, tendo também exames como o tricograma, cultura fúngica, histopatológico e a lâmpada de wood. Objetivo: Devido ao fato de que todos os fungos relatados neste trabalho possuem a capacidade de transmissão para os seres humanos, a dermatofitose torna-se um caso de saúde pública. Estes fungos possuem distribuição mundial, todavia, há uma predileção por regiões de clima mais quente e úmido, como o Brasil. O controle dessa dermatopatia, sendo necessário o conhecimento sobre a sua etiologia, epidemiologia, sinais clínicos e os meios de diagnósticos disponíveis, para que se consiga um tratamento adequado, prevenção e controle. Animais acometidos podem apresentar áreas de alopecia circulares, sendo elas isoladas ou multifocais, com descamação, podendo apresentar eritema, com prurido ou não. Os gatos são mais susceptíveis a carrear o fungo de maneira assintomática pois na superfície de sua pele há a presença de um emulsificado lipídico que consegue inibir a patogenicidade, porém, também podem ter sintomatologia, principalmente quando o animal é imunossuprimido, como nos casos dos portadores de FIV e FELV. Métodos: Estudo realizado por meio de artigos científicos já publicados com a finalidade de melhor compreensão, possibilitando discorrer sobre o tema. Resultados: Os estudos mostram que a manifestação sintomatológica difere em cães e gatos, porém ambos podem ser reservatórios da doença. Os gatos por possuírem, em sua grande maioria, um maior acesso a rua, podem se tornar o principal carreador. O fator predisponente para a incidência dos casos está diretamente ligado com o clima que o Brasil possui. Conclusão: Dado o exposto, é possível compreender que a dermatofitose possui um meio vasto de proliferação, pois acomete diversas espécies e em alguns casos, como o dos gatos, a forma assintomática de manifestação facilita a disseminação. Todavia, todos os animais podem funcionar como reservatórios, e com o aumento da integração dos animais de companhia no âmbito familiar, de uma maneira cada vez mais afetiva, pode aumentar o índice de infecções nos seres humanos. PALAVRAS-CHAVE: Dermatopatia, Dermatofitose, Zoonose
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Reports on the topic "Microsporea"

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Firon, Nurit, Prem Chourey, Etan Pressman, Allen Hartwell, and Kenneth J. Boote. Molecular Identification and Characterization of Heat-Stress-Responsive Microgametogenesis Genes in Tomato and Sorghum - A Feasibility Study. United States Department of Agriculture, October 2007. http://dx.doi.org/10.32747/2007.7591741.bard.

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Exposure to higher than optimal temperatures - heat-stress (HS) - is becoming increasingly common to all crop plants worldwide. Heat stress coinciding with microgametogenesis, especially during the post-meiotic phase that is marked by starch biosynthesis, is often associated with starch-deficient pollen and male sterility and ultimately, greatly reduced crop yields. The molecular basis for the high sensitivity of developing pollen grains, on one hand, and factors involved in pollen heat-tolerance, on the other, is poorly understood. The long-term goal of this project is to provide a better understanding of the genes that control pollen quality under heat-stress conditions. The specific objectives of this project were: (1) Determination of the threshold heat stress temperature(s) that affects tomato and sorghum pollen quality whether: a) Chronic mild heat stress conditions (CMHS), or b) Acute heat stress (AHS). (2) Isolation of heat-responsive, microgametogenesis-specific sequences. During our one-year feasibility project, we have accomplished the proposed objectives as follows: Objectrive 1: We have determined the threshold HS conditions in tomato and sorghum. This was essential for achieving the 2nd objective, since our accumulated experience (both Israeli and US labs) indicate that when temperature is raised too high above "threshold HS levels" it may cause massive death of the developing pollen grains. Above-threshold conditions have additional major disadvantages including the "noise" caused by induced expression of genes involved in cell death and masking of the differences between heatsensitive and heat-tolerant pollen grains. Two different types of HS conditions were determined: a) Season-long CMHS conditions: 32/26°C day/night temperatures confirmed in tomato and 36/26°C day maximum/night minimum temperatures in sorghum. b) Short-term AHS: In tomato, 2 hour exposure to 42-45°C (at 7 to 3 days before anthesis) followed by transfer to 28/22±2oC day/night temperatures until flower opening and pollen maturation, caused 50% reduced germinating pollen in the heat-sensitive 3017 cv.. In sorghum, 36/26°C day/night temperatures 10 to 5 days prior to panicle emergence, occurring at 35 days after sowing (DAS) in cv. DeKalb28E, produced starch-deficient and sterile pollen. Objective 2: We have established protocols for the high throughput transcriptomic approach, cDNA-AFLP, for identifying and isolating genes exhibiting differential expression in developing microspores exposed to either ambient or HS conditions and created a databank of HS-responsivemicrogametogenesis-expressed genes. A subset of differentially displayed Transcript-Derived Fragments (TDFs) that were cloned and sequenced (35 & 23 TDFs in tomato and sorghum, respectively) show close sequence similarities with metabolic genes, genes involved in regulation of carbohydrate metabolism, genes implicated in thermotolerance (heat shock proteins), genes involved in long chain fatty acids elongation, genes involved in proteolysis, in oxidation-reduction, vesicle-mediated transport, cell division and transcription factors. T-DNA-tagged Arabidopsis mutants for part of these genes were obtained to be used for their functional analysis. These studies are planned for a continuation project. Following functional analyses of these genes under HS – a valuable resource of genes, engaged in the HS-response of developing pollen grains, that could be modulated for the improvement of pollen quality under HS in both dicots and monocots and/or used to look for natural variability of such genes for selecting heat-tolerant germplasm - is expected.
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