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Journal articles on the topic 'Microspectrophotometry'

Author: Grafiati

Published: 4 June 2021

Last updated: 4 May 2024

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1

Hulting, A. L., U. Askensten, B. Tribukait, J. Wersäll, G. Auer, L. Grimelius, S. Falkmer, and S. Werner. "DNA evaluation in growth hormone producing pituitary adenomas: flow cytometry versus single cell analysis." Acta Endocrinologica 121, no. 3 (September 1989): 317–21. http://dx.doi.org/10.1530/acta.0.1210317.

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Abstract. DNA patterns were analysed in 26 GH-producing pituitary adenomas by flow cytometry as well as by microspectrophotometry. Twelve tumours (46%) were diploid according to both methods, whereas 5 tumours (19%) showed aneuploid DNA patterns. Nine tumours were classified differently by the two methods: flow cytometry resulted in diploidy in 2 and aneuploidy in 7 patients, whereas microspectrophotometry showed diploidy in 5 tumours, tetraploidy in 3 and aneuploidy in 1. Methodological limitations may explain the discrepancy in the results obtained by the two methods. However, both the flow cytometry and the microspectrophotometry method show the presence of aneuploid DNA patterns in GH-producing pituitary adenomas despite their benign growth characteristics and the clinically benign course of the disease. This comparative study with two methods measuring DNA content, shows that depending on the criteria used for diploidy-aneuploidy, the freqency of aneuploidy will vary. In this material of 26 GH-producing adenomas, 46% were aneuploid according to flow cytometry and 23% according to microspectrophotometric. However, no correlation to tumour size or GH levels was found with either method when patients with aneuploid and diploid tumours were compared. Therefore, no clinial significance can so far be drawn from these results.

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2

Bourgeois, Dominique, Xavier Vernede, Virgile Adam, Emanuela Fioravanti, and Thomas Ursby. "A microspectrophotometer for UV–visible absorption and fluorescence studies of protein crystals." Journal of Applied Crystallography 35, no. 3 (May 16, 2002): 319–26. http://dx.doi.org/10.1107/s0021889802003837.

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Absorption microspectrophotometry has been shown to be of considerable help to probe crystalline proteins containing chromophores, metal centres, or coloured substrates/co-factors. Absorption spectra contribute to the proper interpretation of crystallographic structures, especially when transient intermediate states are studied. Here it is shown that fluorescence microspectrophotometry might also be used for such purposes if endogenous fluorophores are present in the macromolecule or when exogenous fluorophores are added and either bind to the protein or reside in the solvent channels. An off-line microspectrophotometer that is able to perform low-temperature absorption and fluorescence spectroscopy on crystals mounted in cryo-loops is described. One-shot steady-state emission spectra of outstanding quality were routinely collected from several samples. In some cases, crystals with optical densities that are too low or too high for absorption studies can still be tackled with fluorescence microspectrophotometry. The technique may be used for simple controls such as checking the presence, absence or redox state of a fluorescent substrate/co-factor. Potential applications in the field of kinetic crystallography are numerous. In addition, the possibility to probe key physico-chemical parameters of the crystal, such as temperature, pH or solvent viscosity, could trigger new studies in protein dynamics.

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3

Bergvinson, D. J., J. T. Arnason, and L. N. Pietrzak. "Localization and quantification of cell wall phenolics in European corn borer resistant and susceptible maize inbreds." Canadian Journal of Botany 72, no. 9 (September 1, 1994): 1243–49. http://dx.doi.org/10.1139/b94-152.

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Three maize inbreds (MBR 6796-15, B86, and CI31A) resistant to leaf feeding by the European corn borer Ostrinia nubilalis, and one susceptible inbred (MS72), were evaluated for insect resistance and phytochemical composition to gain a better understanding of maize-resistance mechanisms. Insect resistance was evaluated using laboratory bioassays that demonstrated that immature leaf tissue is the preferred feeding substrate. Phytochemical analyses were conducted for leaf protein, hydroxamic acid content, and hydroxycinnamic acids bound to the cell wall for both immature and mature leaf tissue. Hydroxycinnamic acid distribution in cell walls was examined using five stains, autofluorescence, and microspectrophotometry. Phloroglucinol, azure B, diazotized salts of p-nitroaniline and sulfanilic acid, and chlorine sulfite allowed visualization of phenolic localization but were not quantitative. Microspectrophotometer readings of epidermal, phloem, and xylem cell walls confirmed staining results, showing extremely low cell wall hydroxycinnamic acid levels in epidermal cell walls of immature leaf tissue. Foliar nitrogen content was not related to insect feeding preference. Hydroxycinnamic acid fortification of epidermal cell walls appears to correlate best with corn borer feeding preference, accounting for differential resistance between inbred lines and between tissue maturities. Microspectrophotometry may be useful as a technique for monitoring phytochemical resistance mechanisms in breeding programs. Key words: maize, Ostrinia nubilalis, phenolic, hydroxycinnamic acids, cell wall, microspectrophotometry, resistance.

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Carpentier, Philippe, Antoine Royant, Jérémy Ohana, and Dominique Bourgeois. "Advances in spectroscopic methods for biological crystals. 2. Raman spectroscopy." Journal of Applied Crystallography 40, no. 6 (November 10, 2007): 1113–22. http://dx.doi.org/10.1107/s0021889807044202.

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A Raman microspectrophotometer is described that allows the spectroscopic investigation of protein crystals under exactly the same conditions as those used for X-ray data collection. The concept is based on the integration of the Raman excitation/collection optics into a microspectrophotometer built around a single-axis diffractometer and a cooling device. It is shown that Raman spectra of outstanding quality can be recorded from crystallized macromolecules under non-resonant conditions. It is proposed that equipment developed in the context of macromolecular cryocrystallography, such as commonly used cryoloops, can be advantageously used to improve the quality of Raman spectra. In a few examples, it is shown that Raman microspectrophotometry provides crucial complementary information to X-ray crystallography,e.g.identifying the chemical nature of unknown features discovered in electron-density maps, or following ligand-binding kinetics in biological crystals. The feasibility of `online' Raman measurements performed directly on the ESRF macromolecular crystallography beamlines has been investigated and constitutes a promising perspective for the routine implementation of combined spectroscopic and crystallographic methods.In crystalloRaman spectroscopy efficiently complements absorption/fluorescence microspectrophotometry for the study of biological crystals and opens up new avenues for difficult structural projects with mechanistic perspectives in the field of protein crystallography.

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5

Ronda, Luca, Stefano Bruno, Stefano Bettati, and Andrea Mozzarelli. "Protein crystal microspectrophotometry." Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1814, no. 6 (June 2011): 734–41. http://dx.doi.org/10.1016/j.bbapap.2010.12.008.

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6

Schmidt, Werner, Paul Galland, Horst Senger, and Masaki Furuya. "Microspectrophotometry ofEuglena gracilis." Planta 182, no. 3 (October 1990): 375–81. http://dx.doi.org/10.1007/bf02411388.

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7

Pearson, Arwen R., Andrea Mozzarelli, and Gian Luigi Rossi. "Microspectrophotometry for structural enzymology." Current Opinion in Structural Biology 14, no. 6 (December 2004): 656–62. http://dx.doi.org/10.1016/j.sbi.2004.10.007.

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8

Ghoshal, Amitabh, Daniel G. DeMartini, Elizabeth Eck, and Daniel E. Morse. "Optical parameters of the tunable Bragg reflectors in squid." Journal of The Royal Society Interface 10, no. 85 (August 6, 2013): 20130386. http://dx.doi.org/10.1098/rsif.2013.0386.

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Cephalopods (e.g. octopus, squid and cuttlefish) dynamically tune the colour and brightness of their skin for camouflage and communication using specialized skin cells called iridocytes. We use high-resolution microspectrophotometry to investigate individual tunable Bragg structures (consisting of alternating reflectin protein-containing, high-refractive index lamellae and low-refractive index inter-lamellar spaces) in live and chemically fixed iridocytes of the California market squid, Doryteuthis opalescens . This subcellular, single-stack microspectrophotometry allows for spectral normalization, permitting use of a transfer-matrix model of Bragg reflectance to calculate all the parameters of the Bragg stack—the refractive indices, dimensions and numbers of the lamellae and inter-lamellar spaces. Results of the fitting analyses show that eight or nine pairs of low- and high-index layers typically contribute to the observed reflectivity in live cells, whereas six or seven pairs of low- and high-index layers typically contribute to the reflectivity in chemically fixed cells. The reflectin-containing, high-index lamellae of live cells have a refractive index proportional to the peak reflectivity, with an average of 1.405 ± 0.012 and a maximum around 1.44, while the reflectin-containing lamellae in fixed tissue have a refractive index of 1.413 ± 0.015 suggesting a slight increase of refractive index in the process of fixation. As expected, incremental changes in refractive index contribute to the greatest incremental changes in reflectivity for those Bragg stacks with the most layers. The excursions in dimensions required to tune the measured reflected wavelength from 675 (red) to 425 nm (blue) are a decrease from ca 150 to 80 nm for the high-index lamellae and from ca 120 to 50 nm for the low-index inter-lamellar spaces. Fixation-induced dimensional changes also are quantified, leading us to suggest that further microspectrophotometric analyses of this iridocyte system can be used as a model system to quantify the effects of various methods of tissue fixation. The microspectrophotometry technique described can be expected to provide deeper insights into the molecular and physical mechanisms governing other biophotonically active cells and structures.

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9

Boudreaux, Mary K., and Shirley Ebbe. "Evaluation of ploidy of mature canine megakaryocytes, using Feulgen staining and microspectrophotometry." American Journal of Veterinary Research 57, no. 10 (October 1, 1996): 1434–37. http://dx.doi.org/10.2460/ajvr.1996.57.10.1434.

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Abstract Objective To evaluate megakaryocyte size and ploidy, using Feulgen staining and microspectrophotometry, in adult dogs with normal platelet count. Animals Group A contained 8 and group B contained 11 adult dogs. Procedure Megakaryocytes were evaluated by light microscopy and staged according to maturation status. Stage-III megakaryocytes were measured and mapped for future relocation. Bone marrow aspirates were destained and restained, using the Feulgen method. Previously identified stage-III megakaryocytes were measured for DNA content, using microspectrophotometry. Results Megakaryocyte size correlated with ploidy values, and mean sizes within ploidy groups were significantly (P < 0.05) different from each other for both groups. The modal ploidy value of stage-III megakaryocytes, which represented 18% of the total megakaryocyte population of the combined groups, was 32N. This is in contrast to results of flow cytometric studies, which indicated that the modal ploidy value for all canine megakaryocytes was 16N. Conclusions Reasons for the disparate results between microspectrophotometric techniques and flow cytometry include maturation stage of the megakaryocyte population evaluated and percentage of megakaryocytes within that maturation stage. Flow cytometric methods, which evaluate all megakaryocytes detectable by antibody, may include cells still capable of DNA synthesis, resulting in a shift in the observed modal ploidy value. Recognition of the difference between canine and human megakaryocyte ploidy distribution is important, particularly in studies in which the dog is used as an animal model for human megakaryocytopoiesis. (Am J Vet Res 1996;57:1434–1437)

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10

Willmann, Stefan, Hans-Joachim Schwarzmaier, Albert Terenji, Ilya V. Yaroslavsky, and Peter Hering. "Quantitative microspectrophotometry in turbid media." Applied Optics 38, no. 22 (August 1, 1999): 4904. http://dx.doi.org/10.1364/ao.38.004904.

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11

PETRY, HEYWOOD M., and FERENC I. HÁROSI. "Tree Shrew Visual Pigments by Microspectrophotometry." Annals of the New York Academy of Sciences 494, no. 1 Third Colloqu (May 1987): 250–52. http://dx.doi.org/10.1111/j.1749-6632.1987.tb29538.x.

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12

Zeichner, Arie, Nadav Levin, Asne Klein, and Yehuda Novoselsky. "Transmission and Reflectance Microspectrophotometry of Inks." Journal of Forensic Sciences 33, no. 5 (September 1, 1988): 12551J. http://dx.doi.org/10.1520/jfs12551j.

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13

McGeehan, John, Raimond B. G. Ravelli, James W. Murray, Robin Leslie Owen, Florent Cipriani, Sean McSweeney, Martin Weik, and Elspeth F. Garman. "Colouring cryo-cooled crystals: online microspectrophotometry." Journal of Synchrotron Radiation 16, no. 2 (February 25, 2009): 163–72. http://dx.doi.org/10.1107/s0909049509001629.

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X-rays can produce a high concentration of radicals within cryo-cooled macromolecular crystals. Some radicals have large extinction coefficients in the visible (VIS) range of the electromagnetic spectrum, and can be observed optically and spectrally. An online microspectrophotometer with high temporal resolution has been constructed that is capable of measuring UV/VIS absorption spectra (200–1100 nm) during X-ray data collection. The typical X-ray-induced blue colour that is characteristic of a wide range of cryo-conditions has been identified as trapped solvated electrons. Disulphide-containing proteins are shown to form disulphide radicals at millimolar concentrations, with absorption maxima around 400 nm. The solvated electrons and the disulphide radicals seem to have a lifetime in the range of seconds up to minutes at 100 K. The temperature dependence of the kinetics of X-ray-induced radical formation is different for the solvated electrons compared with the disulphide radicals. The online microspectrophotometer provides a technique complementary to X-ray diffraction for analysing and characterizing intermediates and redox states of proteins and enzymes.

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14

Almer, Johanne, Eleanor Mcansh, and Barbara Doupe. "Forensic Fibre Analysis by UV-Visible Microspectrophotometry." Canadian Society of Forensic Science Journal 43, no. 1 (January 2010): 16–30. http://dx.doi.org/10.1080/00085030.2010.10757617.

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15

Hu, Can, Hongcheng Mei, Hongling Guo, and Jun Zhu. "Color analysis of textile fibers by microspectrophotometry." Forensic Chemistry 18 (May 2020): 100221. http://dx.doi.org/10.1016/j.forc.2020.100221.

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16

Olson, Larry A. "Color Comparison in Questioned Document Examination Using Microspectrophotometry." Journal of Forensic Sciences 31, no. 4 (October 1, 1986): 11910J. http://dx.doi.org/10.1520/jfs11910j.

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17

Wasiluk, Karen R., R. Gary Fulcher, Robert J. Jones, and Burle G. Gengenbach. "Characterization of Starch Granules in Maize Using Microspectrophotometry." Starch - Stärke 46, no. 10 (1994): 369–73. http://dx.doi.org/10.1002/star.19940461002.

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18

Sano, Yuzou, and Ryogo Nakada. "Time Course of the Secondary Deposition of Incrusting Materials on Bordered Pit Membranes in Cryptomeria Japonica." IAWA Journal 19, no. 3 (1998): 285–99. http://dx.doi.org/10.1163/22941932-90001533.

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Bordered pit membranes of Cryptomeria japonica were examined successively from the outermost sapwood to the heartwood by scanning electron microscopy and by ultraviolet microspectrophotometry in an attempt to evaluate the time course of the secondary deposition of incrusting materials and to gain clues to their chemical composition. Scanning electron microscopy revealed that the bordered pit membranes were covered by incrusting materials from the middle layer of the sapwood to the heartwood. Both the amount and the appearance of the deposited incrusting materials differed among four regions of the wood, namely, the middle to inner layer of the sapwood, the innermost layer of the sapwood, the intermediate wood and the heartwood. From our results it appears that, in C. japonica, incrusting materials are deposited on bordered pit membranes by stages over several years. Apparent absorption of ultraviolet light by the bordered pit membranes was detected in the analysis of the innermost layer of the sapwood, the intermediate wood and the heartwood. The incrusting materials deposited in these zones were probably phenolic compounds. However, differences in the manner and extent of the absorption of ultraviolet light were found between these three regions of the wood. The results of microspectrophotometric analysis also suggested the phased deposition of incrusting materials at the bordered pit membranes of C. japonica.

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19

Fujimura, Yoshihiko, Hidetoshi Tsuboi, Tomoe Katoh, Kimikazu Hamano, Kazuhiro Suzuki, and Kensuke Esato. "Quantitative Histochemical Analysis of Arterial Grafts Measured by Microspectrophotometry." Japanese Journal of Cardiovascular Surgery 25, no. 1 (1996): 31–35. http://dx.doi.org/10.4326/jjcvs.25.31.

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20

Nymark, S., R. Frederiksen, M. L. Woodruff, M. C. Cornwall, and G. L. Fain. "Bleaching of mouse rods: microspectrophotometry and suction-electrode recording." Journal of Physiology 590, no. 10 (May 1, 2012): 2353–64. http://dx.doi.org/10.1113/jphysiol.2012.228627.

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21

HIRAOKA, TOSHISUKE, KEI-ICHI HIRAI, and TADASHI UEDA. "Cobalt acetylacetonate-diaminobenzidine reaction for microspectrophotometry of cytochrome oxidase." Acta Histochemica et Cytochemica 19, no. 4 (1986): 429–36. http://dx.doi.org/10.1267/ahc.19.429.

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22

Akin, D. E. "UV Absorption Microspectrophotometry and Histochemistry of Flax and Kenaf." Microscopy and Microanalysis 4, S2 (July 1998): 846–47. http://dx.doi.org/10.1017/s1431927600024351.

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Flax (Linum usitatissimum L.) and kenaf (Hibiscus cannabinus L.) are the sources of fibers used for textiles and other industrial applications. Both flax and kenaf produce fibers in the bast region (Fig. 1, 2) which must be separated from other tissues by retting. Although both flax and kenaf are bast fibers, their properties are vastly different. UV absorption microspectrophotometry and histochemistry elucidate their chemistry and structure related to enzymatic retting.Aromatics such as lignins are produced by plants for protection and strength, but their presence inhibits microbial degradation, which is necessary in retting. Histochemical tests indicated variations in the site and type of aromatics within these two plants (1,2). In flax, acid phloroglucinol but not chlorine-sulfite gave positive reactions occasionally in fiber cell walls in the bast. The other cell walls in the bast did not contain aromatics by these tests, although aromatics occurred in the cuticle.

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23

Hooker, R. H., K. E. Creer, and J. S. Brennan. "Microspectrophotometry in the development and photography of fluorescing marks." Forensic Science International 51, no. 2 (October 1991): 297–304. http://dx.doi.org/10.1016/0379-0738(91)90195-o.

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24

Akin, D. E., L. L. Rigsby, W. H. Morrison, A. Sethuraman, and K. E. L. Eriksson. "EM and microspectrophotometric analysis of biological delignification of plants." Proceedings, annual meeting, Electron Microscopy Society of America 53 (August 13, 1995): 986–87. http://dx.doi.org/10.1017/s0424820100141305.

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Aromatic constituents such as lignin bind to carbohydrates within plant cell walls and thus render the plant carbohydrates less utilizable as food and energy. Chemical methods used to upgrade the quality of plant biomass are costly, expensive, and unsafe. White rot fungi, which are the only known microorganisms that, to any extent, can remove lignin from plant cell walls, offer a biological solution to upgrading plant quality. Microscopic analyses provide information on the site of delignification that is strategically important in improving use of plant biomass.Stems of grasses and a legume were treated with the white rot fungi Ceriporiopsis subvermispora and Cyathus stercoreus for 6 weeks. Treated residues were analyzed for structural modifications using scanning and transmission electron microscopy and for aromatic constituents using ultraviolet (UV) absorption microspectrophotometry and gas chromatography. These modifications were related to improved utilization of cell walls by rumen microorganisms.UV absorption microspectrophotometry, in conjunction with gas-chromatography of alkali-treated plants, indicated that ester-linked ferulic and ρ-coumaric acids were particularly susceptible to removal by both fungal species.

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25

Pearson, A. R., T. De la Mora Rey, K. T. Watts, E. Hoeffner, and C. M. Wilmot. "Tracking X-ray derived redox changes using single crystal microspectrophotometry." Acta Crystallographica Section A Foundations of Crystallography 61, a1 (August 23, 2005): c195. http://dx.doi.org/10.1107/s0108767305091683.

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26

Mahnert, K. C., S. Adamopoulos, G. Koch, and H. Militz. "UV-microspectrophotometry: A method to prove wood modification with MMF?" International Wood Products Journal 6, no. 1 (November 13, 2014): 27–30. http://dx.doi.org/10.1179/2042645314y.0000000087.

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27

Kotowski, Thomas M., and Michael C. Grieve. "The Use of Microspectrophotometry to Characterize Microscopic Amounts of Blood." Journal of Forensic Sciences 31, no. 3 (July 1, 1986): 11115J. http://dx.doi.org/10.1520/jfs11115j.

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28

Reed, Hayley, and Suzanne V. Smith. "Microspectrophotometry for detection of metal ion concentration of radioisotopic solutions." Nuclear Medicine and Biology 41, no. 7 (August 2014): 649–50. http://dx.doi.org/10.1016/j.nucmedbio.2014.05.119.

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29

Kopchick, Kristin A., and Christopher R. Bommarito. "Color Analysis of Apparently Achromatic Automotive Paints by Visible Microspectrophotometry." Journal of Forensic Sciences 51, no. 2 (March 2006): 340–43. http://dx.doi.org/10.1111/j.1556-4029.2006.00050.x.

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30

Barrett, Julie A., Jay A. Siegel, and John V. Goodpaster. "Forensic Discrimination of Dyed Hair Color: I. UV-Visible Microspectrophotometry." Journal of Forensic Sciences 55, no. 2 (March 2010): 323–33. http://dx.doi.org/10.1111/j.1556-4029.2009.01306.x.

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31

Marsden, N. V. B. "Red Blood Cells, Phase Contrast, Interference Contrast Microscopy and Microspectrophotometry." Upsala Journal of Medical Sciences 100, no. 1 (January 1995): 33–40. http://dx.doi.org/10.3109/03009739509178894.

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32

Li, Biao. "An Examination of the Sequence of Intersecting Lines using Microspectrophotometry." Journal of Forensic Sciences 61, no. 3 (January 6, 2016): 809–14. http://dx.doi.org/10.1111/1556-4029.13022.

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33

Martyna, Agnieszka, David Lucy, Grzegorz Zadora, Beata M. Trzcinska, Daniel Ramos, and Andrzej Parczewski. "The evidential value of microspectrophotometry measurements made for pen inks." Analytical Methods 5, no. 23 (2013): 6788. http://dx.doi.org/10.1039/c3ay41622d.

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34

Hawryshyn, Craig W., and Ferenc I. Harosi. "Ultraviolet photoreception in carp: Microspectrophotometry and behaviorally determined action spectra." Vision Research 31, no. 3 (January 1991): 567–76. http://dx.doi.org/10.1016/0042-6989(91)90107-g.

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35

Hu, Can, Hongling Guo, Hongcheng Mei, and Jun Zhu. "Prediction of iron content in soil based on microspectrophotometry analysis." Forensic Science International 318 (January 2021): 110600. http://dx.doi.org/10.1016/j.forsciint.2020.110600.

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36

Hartshorne, A. W., and D. K. Laing. "The definition of colour for single textile fibres by microspectrophotometry." Forensic Science International 34, no. 1-2 (May 1987): 107–29. http://dx.doi.org/10.1016/0379-0738(87)90090-9.

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37

Hartshorne, A. W., and D. K. Laing. "An absorption standard for microspectrophotometry: Results of a collaborative exercise." Forensic Science International 51, no. 2 (October 1991): 263–72. http://dx.doi.org/10.1016/0379-0738(91)90191-k.

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38

Kuzuhara, A., and T. Hori. "Diffusion behavior of reducing agents into keratin fibers using microspectrophotometry." Journal of Applied Polymer Science 94, no. 3 (2004): 1131–38. http://dx.doi.org/10.1002/app.21019.

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39

Röder, Thomas, Gerald Koch, and Herbert Sixta. "Application of confocal Raman spectroscopy for the topochemical distribution of lignin and cellulose in plant cell walls of beech wood (Fagus sylvatica L.) compared to UV microspectrophotometry." Holzforschung 58, no. 5 (August 1, 2004): 480–82. http://dx.doi.org/10.1515/hf.2004.072.

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Abstract In a comparative study, the topochemical distribution of lignin in individual cell wall layers of beech wood tissue was determined by confocal Raman spectroscopy and scanning UV microspectrophotometry. The Raman technique was additionally applied to the determination of cellulose content in individual wall layers. In good agreement, the two methods showed maxima of lignin distribution in middle lamellae and cell corners, along with a minimum of cellulose content.

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40

Śmigiel-Kamińska, Daria, Jolanta Wąs-Gubała, and Jolanta Kumirska. "Comparative Studies of Changes in Cotton Fabrics and Fibers under the Influence of Disinfection, Sterilization, and DNA Degradation Agents." Fibers 11, no. 12 (November 22, 2023): 100. http://dx.doi.org/10.3390/fib11120100.

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The purpose of this study was to detect changes in the structure and chemical composition of undyed and dyed cotton fabrics under the influence of six popular agents for disinfection, sterilization, and DNA degradation with different chemical compositions. The original and exposed fabrics and their constituent fibers were subjected to comparative analysis using various optical microscopy methods, infrared spectroscopy, and UV–Vis microspectrophotometry in order to differentiate the exanimated material due to the agents applied. Differences in color, from a slight change to complete discoloration, and in the structure of the tested fabrics, which became more rigid, brittle, or, for example, compact, were noticed. With the use of ATR FTIR, it was possible to identify the presence in the exposed fabrics of residues of these agents that contained quaternary ammonium salts. Bright-field microscopy made it possible to show, above all, changes or lack thereof in the fluorescence properties of single exposed fibers in relation to control ones. With the use of UV–Vis microspectrophotometry, changes in colored fibers following the action of a specific agent on the examined fabrics were monitored. A case study was presented as an application aspect of the research, in which the use of concrete disinfectants was recognized based on changes observed in cotton clothing.

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41

Golemis, Dean. "Editorial | The Microscopy of Degradation." Microscope 69, no. 1 (2022): ii. http://dx.doi.org/10.59082/kldd3811.

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Forensic casework often involves the examination of dyed man-made fabrics and fibers, which are often degraded by environmental conditions, especially prolonged exposure to ultraviolet (UV) radiation from sunlight and artificial light. But little is known about how the dyed fibers are affected by UV light sources. Enter UV-visible microspectrophotometry (UV-Vis MSP), the preferred microanalytical technique to study small sections of these fibers, preferably without inflicting further damage to them.

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42

Peracchi, Alessio, Andrea Mozzarelli, Gian Luigi Rossi, Paola Dominicit, and Carla Borri Voltattornit. "Single Crystal Polarized Absorption Microspectrophotometry of Aromatic L-Amino Acid Decarboxylase." Protein & Peptide Letters 1, no. 2 (September 1994): 98–105. http://dx.doi.org/10.2174/0929866501666220424132804.

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Microspectrophotometric measurements on single crystals of aromatic amino acid decarboxylase from pig kidney show that the enzyme -bound pyridoxal-5'-phosphate exhibits the same spectrum in the crystal as it does in solution. Substrates and substrate analogs diffuse into the crystal and bind at the active site giving rise to spectral changes similar to those previously observed for the enzyme in solution. These studies indicate that the crystallization of pig kidney AADC does not alter the properties of the active site.

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Stark, William S., Kent D. Walker, and J. Marc Eidel. "Ultraviolet and blue light induced damage to theDrosophilaretina: Microspectrophotometry and electrophysiology." Current Eye Research 4, no. 10 (January 1985): 1059–75. http://dx.doi.org/10.3109/02713688509003351.

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Tokuhisa, T., M. Murakami, and T. Kouyama. "3H1345 structural analysis of reaction intermediates of bacteriorhodopsin by cryogenic microspectrophotometry." Seibutsu Butsuri 42, supplement2 (2002): S170. http://dx.doi.org/10.2142/biophys.42.s170_2.

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Akin, D. E., L. L. Rigsby, W. S. Borneman, and R. D. Hartley. "UV absorption microspectrophotometry of plant cell walls in relation to biodegradation." Proceedings, annual meeting, Electron Microscopy Society of America 50, no. 1 (August 1992): 864–65. http://dx.doi.org/10.1017/s0424820100124720.

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Abstract:

A major limitation to the biodegradation of plant cell walls is the presence of phenolic compounds, which covalently link to carbohydrates and render these potentially digestible components unavailable for microbial utilization. A more detailed understanding of phenolic compounds and their association within specific cell types is required for development of strategies to enhance biodegradation of plant fiber for efficient utilization of foods and feeds. UV absorption microspectrophotometry was employed to characterize the phenolics within cell walls of a series of plants with different biodegradabilities. Scanning electron microscopy was used to assess the degradation of specific cell types incubated with fiber-digesting microorganisms from the rumen ecosystem.

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Akin, Danny E., and Roy D. Hartley. "Microspectrophotometry and Digestibility of Alkali‐Treated Walls in Bermudagrass Cell Types." Crop Science 32, no. 5 (September 1992): 1116–22. http://dx.doi.org/10.2135/cropsci1992.0011183x003200050009x.

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Sillman, A. J., S. J. Ronan, and E. R. Loew. "Scanning electron microscopy and microspectrophotometry of the photoreceptors of ictalurid catfishes." Journal of Comparative Physiology A 173, no. 6 (December 1993): 801–7. http://dx.doi.org/10.1007/bf02451910.

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Aitken, Colin, Ya-Ting Chang, Patrick Buzzini, Grzegorz Zadora, and Genevieve Massonnet. "The evaluation of evidence for microspectrophotometry data using functional data analysis." Forensic Science International 305 (December 2019): 110007. http://dx.doi.org/10.1016/j.forsciint.2019.110007.

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Kuzuhara, A., and T. Hori. "Diffusion behavior of poly(ethylene imine) into keratin fibers using microspectrophotometry." Journal of Applied Polymer Science 97, no. 1 (2005): 65–71. http://dx.doi.org/10.1002/app.21731.

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50

COLLIN, SHAUN P., NATHAN S. HART, JULIA SHAND, and IAN C. POTTER. "Morphology and spectral absorption characteristics of retinal photoreceptors in the southern hemisphere lamprey (Geotria australis)." Visual Neuroscience 20, no. 2 (March 2003): 119–30. http://dx.doi.org/10.1017/s0952523803202030.

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The morphology and spectral absorption characteristics of the retinal photoreceptors in the southern hemisphere lamprey Geotria australis (Agnatha) were studied using light and electron microscopy and microspectrophotometry. The retinae of both downstream and upstream migrants of Geotria contained two types of cone photoreceptor and one type of rod photoreceptor. Visual pigments contained in the outer segments of these three photoreceptor types had absorbance spectra typical of porphyropsins and with wavelengths of maximum absorbance (downstream/ upstream) at 610/616 nm (long-wavelength-sensitive cone, LWS), 515/515 nm (medium-wavelength-sensitive cone, MWS), and 506/500 nm (medium-wavelength-sensitive rod). A “yellow” photostable pigment was present in the myoid region of all three types of photoreceptor in the downstream migrant. The same short-wavelength-absorbing pigment, which prevents photostimulation of the beta band of the visual pigment in the outer segment, was present in the rods and LWS cones of the upstream migrant, but was replaced by a large transparent ellipsosome in the MWS cones. Using microspectrophotometric and anatomical data, the quantal spectral sensitivity of each photoreceptor type was calculated. Our results provide the first evidence of a jawless vertebrate, represented today solely by the lampreys and hagfishes, with two morphologically and physiologically distinct types of cone photoreceptors, in addition to a rod-like photoreceptor containing a colored filter (a cone-like characteristic). In contrast, all other lampreys studied thus far have either (1) one type of cone and one type of rod, or (2) a single type of rod-like photoreceptor.

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