Relevant bibliographies by topics / Microspectrophotometry

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Microspectrophotometry'

Author: Grafiati
Published: 4 June 2021
Last updated: 4 May 2024

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Journal articles on the topic "Microspectrophotometry"

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Hulting, A. L., U. Askensten, B. Tribukait, J. Wersäll, G. Auer, L. Grimelius, S. Falkmer, and S. Werner. "DNA evaluation in growth hormone producing pituitary adenomas: flow cytometry versus single cell analysis." Acta Endocrinologica 121, no. 3 (September 1989): 317–21. http://dx.doi.org/10.1530/acta.0.1210317.

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Abstract. DNA patterns were analysed in 26 GH-producing pituitary adenomas by flow cytometry as well as by microspectrophotometry. Twelve tumours (46%) were diploid according to both methods, whereas 5 tumours (19%) showed aneuploid DNA patterns. Nine tumours were classified differently by the two methods: flow cytometry resulted in diploidy in 2 and aneuploidy in 7 patients, whereas microspectrophotometry showed diploidy in 5 tumours, tetraploidy in 3 and aneuploidy in 1. Methodological limitations may explain the discrepancy in the results obtained by the two methods. However, both the flow cytometry and the microspectrophotometry method show the presence of aneuploid DNA patterns in GH-producing pituitary adenomas despite their benign growth characteristics and the clinically benign course of the disease. This comparative study with two methods measuring DNA content, shows that depending on the criteria used for diploidy-aneuploidy, the freqency of aneuploidy will vary. In this material of 26 GH-producing adenomas, 46% were aneuploid according to flow cytometry and 23% according to microspectrophotometric. However, no correlation to tumour size or GH levels was found with either method when patients with aneuploid and diploid tumours were compared. Therefore, no clinial significance can so far be drawn from these results.
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Bourgeois, Dominique, Xavier Vernede, Virgile Adam, Emanuela Fioravanti, and Thomas Ursby. "A microspectrophotometer for UV–visible absorption and fluorescence studies of protein crystals." Journal of Applied Crystallography 35, no. 3 (May 16, 2002): 319–26. http://dx.doi.org/10.1107/s0021889802003837.

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Absorption microspectrophotometry has been shown to be of considerable help to probe crystalline proteins containing chromophores, metal centres, or coloured substrates/co-factors. Absorption spectra contribute to the proper interpretation of crystallographic structures, especially when transient intermediate states are studied. Here it is shown that fluorescence microspectrophotometry might also be used for such purposes if endogenous fluorophores are present in the macromolecule or when exogenous fluorophores are added and either bind to the protein or reside in the solvent channels. An off-line microspectrophotometer that is able to perform low-temperature absorption and fluorescence spectroscopy on crystals mounted in cryo-loops is described. One-shot steady-state emission spectra of outstanding quality were routinely collected from several samples. In some cases, crystals with optical densities that are too low or too high for absorption studies can still be tackled with fluorescence microspectrophotometry. The technique may be used for simple controls such as checking the presence, absence or redox state of a fluorescent substrate/co-factor. Potential applications in the field of kinetic crystallography are numerous. In addition, the possibility to probe key physico-chemical parameters of the crystal, such as temperature, pH or solvent viscosity, could trigger new studies in protein dynamics.
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Bergvinson, D. J., J. T. Arnason, and L. N. Pietrzak. "Localization and quantification of cell wall phenolics in European corn borer resistant and susceptible maize inbreds." Canadian Journal of Botany 72, no. 9 (September 1, 1994): 1243–49. http://dx.doi.org/10.1139/b94-152.

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Three maize inbreds (MBR 6796-15, B86, and CI31A) resistant to leaf feeding by the European corn borer Ostrinia nubilalis, and one susceptible inbred (MS72), were evaluated for insect resistance and phytochemical composition to gain a better understanding of maize-resistance mechanisms. Insect resistance was evaluated using laboratory bioassays that demonstrated that immature leaf tissue is the preferred feeding substrate. Phytochemical analyses were conducted for leaf protein, hydroxamic acid content, and hydroxycinnamic acids bound to the cell wall for both immature and mature leaf tissue. Hydroxycinnamic acid distribution in cell walls was examined using five stains, autofluorescence, and microspectrophotometry. Phloroglucinol, azure B, diazotized salts of p-nitroaniline and sulfanilic acid, and chlorine sulfite allowed visualization of phenolic localization but were not quantitative. Microspectrophotometer readings of epidermal, phloem, and xylem cell walls confirmed staining results, showing extremely low cell wall hydroxycinnamic acid levels in epidermal cell walls of immature leaf tissue. Foliar nitrogen content was not related to insect feeding preference. Hydroxycinnamic acid fortification of epidermal cell walls appears to correlate best with corn borer feeding preference, accounting for differential resistance between inbred lines and between tissue maturities. Microspectrophotometry may be useful as a technique for monitoring phytochemical resistance mechanisms in breeding programs. Key words: maize, Ostrinia nubilalis, phenolic, hydroxycinnamic acids, cell wall, microspectrophotometry, resistance.
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Carpentier, Philippe, Antoine Royant, Jérémy Ohana, and Dominique Bourgeois. "Advances in spectroscopic methods for biological crystals. 2. Raman spectroscopy." Journal of Applied Crystallography 40, no. 6 (November 10, 2007): 1113–22. http://dx.doi.org/10.1107/s0021889807044202.

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A Raman microspectrophotometer is described that allows the spectroscopic investigation of protein crystals under exactly the same conditions as those used for X-ray data collection. The concept is based on the integration of the Raman excitation/collection optics into a microspectrophotometer built around a single-axis diffractometer and a cooling device. It is shown that Raman spectra of outstanding quality can be recorded from crystallized macromolecules under non-resonant conditions. It is proposed that equipment developed in the context of macromolecular cryocrystallography, such as commonly used cryoloops, can be advantageously used to improve the quality of Raman spectra. In a few examples, it is shown that Raman microspectrophotometry provides crucial complementary information to X-ray crystallography,e.g.identifying the chemical nature of unknown features discovered in electron-density maps, or following ligand-binding kinetics in biological crystals. The feasibility of `online' Raman measurements performed directly on the ESRF macromolecular crystallography beamlines has been investigated and constitutes a promising perspective for the routine implementation of combined spectroscopic and crystallographic methods.In crystalloRaman spectroscopy efficiently complements absorption/fluorescence microspectrophotometry for the study of biological crystals and opens up new avenues for difficult structural projects with mechanistic perspectives in the field of protein crystallography.
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Ronda, Luca, Stefano Bruno, Stefano Bettati, and Andrea Mozzarelli. "Protein crystal microspectrophotometry." Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1814, no. 6 (June 2011): 734–41. http://dx.doi.org/10.1016/j.bbapap.2010.12.008.

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Schmidt, Werner, Paul Galland, Horst Senger, and Masaki Furuya. "Microspectrophotometry ofEuglena gracilis." Planta 182, no. 3 (October 1990): 375–81. http://dx.doi.org/10.1007/bf02411388.

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Pearson, Arwen R., Andrea Mozzarelli, and Gian Luigi Rossi. "Microspectrophotometry for structural enzymology." Current Opinion in Structural Biology 14, no. 6 (December 2004): 656–62. http://dx.doi.org/10.1016/j.sbi.2004.10.007.

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Ghoshal, Amitabh, Daniel G. DeMartini, Elizabeth Eck, and Daniel E. Morse. "Optical parameters of the tunable Bragg reflectors in squid." Journal of The Royal Society Interface 10, no. 85 (August 6, 2013): 20130386. http://dx.doi.org/10.1098/rsif.2013.0386.

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Cephalopods (e.g. octopus, squid and cuttlefish) dynamically tune the colour and brightness of their skin for camouflage and communication using specialized skin cells called iridocytes. We use high-resolution microspectrophotometry to investigate individual tunable Bragg structures (consisting of alternating reflectin protein-containing, high-refractive index lamellae and low-refractive index inter-lamellar spaces) in live and chemically fixed iridocytes of the California market squid, Doryteuthis opalescens . This subcellular, single-stack microspectrophotometry allows for spectral normalization, permitting use of a transfer-matrix model of Bragg reflectance to calculate all the parameters of the Bragg stack—the refractive indices, dimensions and numbers of the lamellae and inter-lamellar spaces. Results of the fitting analyses show that eight or nine pairs of low- and high-index layers typically contribute to the observed reflectivity in live cells, whereas six or seven pairs of low- and high-index layers typically contribute to the reflectivity in chemically fixed cells. The reflectin-containing, high-index lamellae of live cells have a refractive index proportional to the peak reflectivity, with an average of 1.405 ± 0.012 and a maximum around 1.44, while the reflectin-containing lamellae in fixed tissue have a refractive index of 1.413 ± 0.015 suggesting a slight increase of refractive index in the process of fixation. As expected, incremental changes in refractive index contribute to the greatest incremental changes in reflectivity for those Bragg stacks with the most layers. The excursions in dimensions required to tune the measured reflected wavelength from 675 (red) to 425 nm (blue) are a decrease from ca 150 to 80 nm for the high-index lamellae and from ca 120 to 50 nm for the low-index inter-lamellar spaces. Fixation-induced dimensional changes also are quantified, leading us to suggest that further microspectrophotometric analyses of this iridocyte system can be used as a model system to quantify the effects of various methods of tissue fixation. The microspectrophotometry technique described can be expected to provide deeper insights into the molecular and physical mechanisms governing other biophotonically active cells and structures.
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Boudreaux, Mary K., and Shirley Ebbe. "Evaluation of ploidy of mature canine megakaryocytes, using Feulgen staining and microspectrophotometry." American Journal of Veterinary Research 57, no. 10 (October 1, 1996): 1434–37. http://dx.doi.org/10.2460/ajvr.1996.57.10.1434.

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Abstract Objective To evaluate megakaryocyte size and ploidy, using Feulgen staining and microspectrophotometry, in adult dogs with normal platelet count. Animals Group A contained 8 and group B contained 11 adult dogs. Procedure Megakaryocytes were evaluated by light microscopy and staged according to maturation status. Stage-III megakaryocytes were measured and mapped for future relocation. Bone marrow aspirates were destained and restained, using the Feulgen method. Previously identified stage-III megakaryocytes were measured for DNA content, using microspectrophotometry. Results Megakaryocyte size correlated with ploidy values, and mean sizes within ploidy groups were significantly (P < 0.05) different from each other for both groups. The modal ploidy value of stage-III megakaryocytes, which represented 18% of the total megakaryocyte population of the combined groups, was 32N. This is in contrast to results of flow cytometric studies, which indicated that the modal ploidy value for all canine megakaryocytes was 16N. Conclusions Reasons for the disparate results between microspectrophotometric techniques and flow cytometry include maturation stage of the megakaryocyte population evaluated and percentage of megakaryocytes within that maturation stage. Flow cytometric methods, which evaluate all megakaryocytes detectable by antibody, may include cells still capable of DNA synthesis, resulting in a shift in the observed modal ploidy value. Recognition of the difference between canine and human megakaryocyte ploidy distribution is important, particularly in studies in which the dog is used as an animal model for human megakaryocytopoiesis. (Am J Vet Res 1996;57:1434–1437)
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Willmann, Stefan, Hans-Joachim Schwarzmaier, Albert Terenji, Ilya V. Yaroslavsky, and Peter Hering. "Quantitative microspectrophotometry in turbid media." Applied Optics 38, no. 22 (August 1, 1999): 4904. http://dx.doi.org/10.1364/ao.38.004904.

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Dissertations / Theses on the topic "Microspectrophotometry"

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Partridge, J. C. "Microspectrophotometry of vertebrate photoreceptors." Thesis, University of Bristol, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373843.

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Hartshorne, A. W. "The characterisation of single fibres in forensic science by microspectrophotometry." Thesis, Heriot-Watt University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380723.

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Chan, Gordon H. "Beta and electron dose imaging using a microspectrophotometer system and radiochromic film /." *McMaster only, 1999.

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Kent, Jeremy. "The visual pigments of deep-sea crustaceans." Thesis, University of Bristol, 1997. http://hdl.handle.net/1983/c15eaa2a-756c-43dc-bf73-c30c09e5d5f2.

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Hart, Nathan Scott. "Avian photoreceptors." Thesis, University of Bristol, 1998. http://hdl.handle.net/1983/f35814d2-b726-4d74-b05d-6fb30c89f894.

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Mosk, Virginia Jan. "The visual system of seahorses and pipefish : a study of visual pigments and other characteristics." University of Western Australia. School of Animal Biology, 2005. http://theses.library.uwa.edu.au/adt-WU2005.0081.

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Syngnathidae (seahorse, pipefish, pipehorses & seadragons) are highly visual feeders with different species feeding on specific types of prey, a behaviour that has been related to snout length. Worldwide, many species have become threatened by habitat destruction, collection for the aquarium trade and exploitation for traditional medicine, as well as recreational and commercial bycatch. Attempts to establish aquaculture programs have been of limited success. Little is known about their visual capabilities in detail. The visual systems of fishes are known to have evolved specific adaptations that can be related to the colour of water in which they live and specific visual tasks such as predator detection and acquisition of food. This study examined the ocular and retinal morphology, photoreceptor structure and spectral sensitivity of adult individuals of a local pipefish (S. argus), local seahorse (Hippocampus subelongatus) which both inhabit green water seagrass beds, and a tropical species of seahorse (Hippocampus barbouri) from blue water coral reefs. Some juveniles were also investigated. Accordingly, we developed an understanding of the features that are common to all syngnathids and those that have evolved for specific environments. Cryosections of the eyes were taken to determine morphological distinctions of this group. Lens characteristics measured using a spectrophotometer determined 50% cut-off wavelengths below 408nm for all 3 species, hence no transmission of UV light to the retina. Histological examination determined a cone dominated fovea in the ventro-temporal retina and very large rods concentrated in the peripheral retina and adjacent to the optic nerve. Microspectrophotometry measured the absorption characteristics of the visual pigments within the photoreceptors showing the presence and maximum sensitivity (λmax) of rods, SWS single cones, and a broad, complex array of LWS double/twin cones. The results are discussed in relation to the light environment inhabited by each species and their feeding requirements. The implications for the design of suitable light environments for aquarium and aquaculture programs for the Syngnathidae are also discussed. Rearing success of this family of fish, for both the aquarium trade and re-stocking programs, would be advised to take lighting regimes and specifics of the animals’ vision into account
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Russo, Rachel. "Hanging by a Thread: Enhancing the Forensic Value of Dyed Cotton Trace Evidence through the Application of Novel Techniques in Fiber Discrimination." Honors in the Major Thesis, University of Central Florida, 2005. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/801.

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This item is only available in print in the UCF Libraries. If this is your Honors Thesis, you can help us make it available online for use by researchers around the world by following the instructions on the distribution consent form at http://library.ucf
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Carvalho, Fernanda Machado Mendes. "Caracterização ultraestrutural e hidrólise enzimática de cana-de-açúcar e bagaço pré-tratado quimio-mecanicamente." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/97/97132/tde-07112014-155359/.

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O presente trabalho tem como objetivo estudar as modificações ocorridas na cana-de-açúcar, com diferentes composições químicas e estruturais, pelo pré-tratamento sulfito alcalino. A remoção de lignina e hemicelulose, bem como a introdução de grupos sulfônicos em cana-de-açúcar que ocorrem durante o pré-tratamento sulfito alcalino tornam mais fácil a hidrólise da celulose. A compreensão das modificações químicas e físicas em materiais lignocelulósicos que ocorrem durante este pré-tratamento é fundamental para a geração de processos mais eficazes. Neste trabalho, bagaço e entrenós de cana-de-açúcar, selecionados de plantas híbridas com composição química variada, foram pré-tratados em condições brandas com 10% de sulfito e 5% de hidróxido de sódio por diferentes tempos. No início do pré-tratamento, a deslignificação aumentou rapidamente, o mesmo não aconteceu com a hemicelulose. Nos primeiros 30 min de pré-tratamento do bagaço de cana-de-açúcar houve remoção de 50% da lignina inicial e 30% da hemicelulose, o que ocasionou uma melhora significativa na conversão de celulose, atingindo 64%. Mesmo sem remoção adicional de lignina e hemicelulose, o processo continuou a introduzir os grupos ácidos, o que contribuiu para o inchamento da fibra. A largura da fibra do bagaço não tratado aumentou de 10,4 ?m para 30 ?m no material pré-tratado com 120 min. Estas modificações na fibra foram responsáveis pelo aumento na eficiência da hidrólise enzimática da celulose, a qual atingiu 92%. Híbridos experimentais com teores reduzidos de lignina apresentaram taxas iniciais de hidrólise mais elevadas e um menor tempo de pré-tratamento para alcançar a conversão total de celulose do que a cana de referência. Diferentes regiões (medula, interface, córtex e fração externa) dos entrenós das canas foram hidrolisadas por celulases. O pré-tratamento da interface, córtex e fração externa com sulfito-alcalino produziu substratos menos recalcitrantes com o aumento do tempo de reação e resultou na melhora da hidrólise enzimática. Foram utilizadas várias técnicas para avaliar as mudanças que ocorreram durante o pré-tratamento, as quais foram capazes de estudar a morfologia da superfície e as características químicas das amostras. O tratamento químico ocasionou uma intensa deslignificação e alterações morfológicas nas superfícies das fibras da cana-de-açúcar. A redução na absorção a 285 nm e 315 nm das paredes celulares das fibras, parênquima e dos vasos aumentou substancialmente os valores de conversão enzimática da celulose e da hemicelulose. Microscopia eletrônica de varredura por emissão de campo (FE-SEM) revelou que as fibras da região do córtex e, especialmente, da interface mostrou paredes celulares colapsadas após a parcial deslignificação. Após o tratamento sulfito alcalino, os dados de espectroscopia fotoelétrica de raio-X (XPS) e espectrometria de massa de íons secundários por tempo de vôo (TOF-SIMS) apresentaram um aumento das intensidades dos sinais nas superfícies das fibras, os quais foram atribuídos à presença de carboidratos em algumas amostras. Em conformidade, os sinais de lignina diminuíram nas superfícies das fibras das mesmas amostras.
The present work aims to study the changes occurring in sugar cane, with different in structure and chemical compositions, by sulfite-alkaline pre-treatment. Removing lignin and hemicellulose as well as introducing sulfonic groups in sugar cane pretreated with alkaline sulfite made cellulose hydrolysis easier. Understanding the chemical and physical alterations occurring during this pretreatment of lignocellulosic materials is fundamental for the generation of effective pretreatment methods. In the present work, sugarcane bagasse and also sugar cane internodes, selected from experimental hybrid plants, were pretreated with the alkaline-sulfite process under mild conditions with varied cooking times. The first 30 min of pretreatment of sugar cane bagasse, which removed approximately half of the initial lignin and 30% of hemicellulose seemed responsible for a significant enhancement of the cellulose conversion level, which reached 64%. After the first 30 min of pretreatment, delignification increased slightly and hemicellulose removal was not enhanced. However, the process continued to introduce acid groups into the residual lignin that enhanced the fiber swelling up to 120 min of cooking. The fiber widths increased from 10,4 ?m in the untreated bagasse to 30 ?m in the 120 min-pretreated material. These changes were responsible for an additional increase in the efficiency of enzymatic hydrolysis of the cellulose, which reached 92%. Experimental hybrids with less original lignin presented higher initial hydrolysis rates than reference sugar cane and required lower time of pretreatment to achieve the total cellulose conversion. Different regions (pith, interface, rind and outermost fraction) of the internodes of types of sugarcanes were hydrolyzed by cellulases. The pretreatment of the interface, rind and outermost fraction with alkaline sulfite produced less recalcitrant substrates with increasing reaction time and resulted in improvement enzymatic hydrolysis. Several techniques enabling the study of surface morphological and chemical characteristics were used to evaluate the changes occurring during the pretreatment step. The chemical treatment caused intense delignification and morphological changes on the sugar cane fiber surfaces. The reduction in the absorption at 285 nm and 315 nm of the cell walls of the fibers, parenchyma and vessel, substantially increased the values of enzymatic conversion of cellulose and hemicellulose. Field emission scanning electron microscopy (FE-SEM) indicated that the fibers from rind regions and especially from the interface showed collapsed cell walls after partial delignification. After the alkaline sulfite treatment, X-ray photoelectrom spectroscopy (XPS) and time-of-flight-secondary ion mass spectrometry (ToF-SIMS) data showed increased signal intensities on the fibers surfaces assigned to carbohydrates of some samples. In accordance, the lignin signals diminished on the fiber surfaces of the same samples.
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Adam, Virgile. "Études mécanistiques des protéines fluorescentes photoactivables : une approche combinée par cristallographie et spectroscopie." Grenoble 1, 2009. http://www.theses.fr/2009GRE10059.

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Depuis la découverte de la protéine fluorescente verte (GFP), celle des protéines fluorescentes photoactivables (PAFPs) a initié une révolution dans le domaine de la technologie des FP. Certaines PAFPs sont capables d'être irréversiblement photo converties d'une couleur à une autre alors que d'autres peuvent être réversiblement commutées entre des formes allumées ou éteintes. Ces protéines son intensivement employées dans les techniques de microscopie optique, particulièrement en "nanoscopie" qui permet d'atteindre une résolution optique 1 0 fois meilleure que la limite d'Abbe. Afin de développer plus en avant ces techniques, la nécessité d'obtenir des sondes fluorescentes plus lumineuses pouvant se photoconvertir ou se photocommuter efficacement est cruciale. En même temps, les marqueurs fluorescents doivent être monomériques et photostables. Pour mieux comprendre les mécanismes des phototransformations des PAFPs, trois membres de la famille ont été étudiés: EosFP, Dendra2 et IrisFP. Le phénomène de photoconversion du vert au rouge de photocommutation réversible et de photoblanchiment ont été étudiés grâce à une combinaison de cristallographie des rayons X et dl microspectrophotométrie, en utilisant le laboratoire Cryobench de l'ESRF/IBS. Les résultats nous ont permis de proposer un mécanisme de photoconversion pour EosFP et Dendra2 et de découvrir et caractériser IrisFP, première PAFP combinant à la fois les propriétés de photoconversion et de photocommutation. Les modifications structurales du chromophore associées à la formation d'un état radicalaire induit par les rayons X, probablement impliqué dans la voie de photoblanchiment des P AFPs, ont aussi été caracterisées
Since the discovery of the green fluorescent protein (GFP), the one of photoactivatable fluorescent proteins (P AFPs), notably from Anthozoan species, triggered a revolution in the field of FP technology. Sorne PAFPs are capable of being irreversibly photoconverted from a green- to a red-emitting form while other ones can be reversibly switched on and off, depending on specific excitation wavelengths. These proteins are being extensively used in optical microscopy techniques, particularly in "nanoscopy", which pro vides optical resolution 10 fold beyond the Abbe limit. Ln order to further develop these techniques, notably in term of time-resolution, the need to obtain brighter fluorescent probes that photoconvert or photoswitch efficiently is crucial. At the same time, fluorescent highlighters generally need to be monomeric and photostable. Ln order to better understand the mechanisms of phototransformations in PAFPs, three members of the family have been studied: EosFP, Dendra2 and IrisFP. The phenomena of green-to-red photoconversion, reversible photoswitching and non-reversible photobleaching have been studied by a combination of X-ray crystallography and microspectrophotometry using the Cryobench laboratory of the ESRF/IBS. Together, the results have a\lowed us to propose a mechanism for the photo conversion of EosFP and Dendra2 and to discover and characterize IrisFP, the first PAFP combining both properties of photoconversion and photoswitching. The structural modifications of the chromophore associated with an X-ray induced radical state, likely to be involved in thé photobleaching pathway of PAFPs, were also characterized
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Chan, Gordon Ho-Chi. "Beta and electron dose imaging using a microspectrophotometer system and radiochromic film." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0030/NQ66259.pdf.

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Books on the topic "Microspectrophotometry"

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Koenig, Jack L. Microspectroscopic imaging of polymers. Washington, DC: American Chemical Society, 1998.

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Microspectroscopy and imaging of polymers. Washington, D.C: American ChemicalSociety, 1997.

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McKittrick, Philip T. The adaptation of selected analytical techniques to vibrational microspectroscopy. 1990.

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Bouffard, Steven P. Novel applications of molecular microspectroscopy. 1994.

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Burlak, Christopher. Analysis of rice blast appressoria using Raman microspectroscopy. 1998.

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Book chapters on the topic "Microspectrophotometry"

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Weigmann, H. D., Y. K. Kamath, and S. B. Ruetsch. "Microspectrophotometry." In Analytical Chemistry of Synthetic Colorants, 133–70. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-1358-8_6.

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Griesbach, R. J. "Applications of microspectrophotometry." In Research Instrumentation for the 21st Century, 175–84. Dordrecht: Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-009-2748-3_9.

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Thomas, E. J., and M. D. Gayeski. "Cryogenic Microspectrophotometry of Myoglobin." In Advances in Experimental Medicine and Biology, 572. Boston, MA: Springer US, 1994. http://dx.doi.org/10.1007/978-1-4615-1875-4_98.

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Bensley, David F., and John C. Crelling. "In-Situ Microspectrophotometry of Coal Macerals." In Advances in Coal Spectroscopy, 119–39. Boston, MA: Springer US, 1992. http://dx.doi.org/10.1007/978-1-4899-3671-4_6.

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Eyring, Michael B. "Fundamentals of Visible Microspectrophotometry in Forensic Science." In Forensic Science Handbook, 245–99. Third edition. | Boca Raton, FL : CRC Press, 2019-: CRC Press, 2020. http://dx.doi.org/10.4324/9781315119939-5.

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Gál, T., T. Veress, and I. Ambrus. "Sample Preparation of Illicit Drugs for FT-IR Microspectrophotometry." In Progress in Fourier Transform Spectroscopy, 377–79. Vienna: Springer Vienna, 1997. http://dx.doi.org/10.1007/978-3-7091-6840-0_87.

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Mozzarelli, Andrea, and Stefano Bettati. "Protein structure-function relationship studied by single crystal polarized absorption microspectrophotometry." In Spectroscopy of Biological Molecules: New Directions, 3–6. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4479-7_1.

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Mozzarelli, Andrea, Barbara Campanini, Stefano Bettati, and Alessio Peracchi. "Functional properties of immobilized pyridoxal 5’-phosphate-dependent enzymes probed by absorption microspectrophotometry." In Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent Proteins, 349–54. Basel: Birkhäuser Basel, 2000. http://dx.doi.org/10.1007/978-3-0348-8397-9_57.

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Shiraishi, Takuo, Kazuhiro Tsujita, and Katsuko Kakinuma. "Microspectrophotometry of Nitric Oxide-Dependent Changes in Hemoglobin in Single Red Blood Cells Incubated with Stimulated Macrophages." In Oxygen Homeostasis and Its Dynamics, 550–56. Tokyo: Springer Japan, 1998. http://dx.doi.org/10.1007/978-4-431-68476-3_69.

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Zade, Anil B., and Kailash N. Munshi. "Sensitive Microspectrophotometric Determination of Some Lanthanides with Bromopyrogallol Red in Presence of Micelle Forming Cationic Surfactants." In Surfactants in Solution, 261–75. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4613-0839-3_20.

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Conference papers on the topic "Microspectrophotometry"

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Lin, Warren, Vincent J. Coates, and Bhanwar Singh. "Measurement of multilayer film and reflectivity on wafers using ultraviolet-visible microspectrophotometry." In Micro - DL Tentative, edited by Michael T. Postek, Jr. SPIE, 1992. http://dx.doi.org/10.1117/12.59833.

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Zimnyakov, Dmitry A., Georgy V. Simonenko, Alexey N. Bashkatov, Elina A. Genina, Nina A. Lakodina, Valery V. Tuchin, and Gregory B. Altshuler. "Spatially resolved microspectrophotometry for hair optical properties and geometry studies: CCD hair tester." In BiOS 2001 The International Symposium on Biomedical Optics, edited by R. Rox Anderson, Kenneth E. Bartels, Lawrence S. Bass, C. Gaelyn Garrett, Kenton W. Gregory, Abraham Katzir, Nikiforos Kollias, et al. SPIE, 2001. http://dx.doi.org/10.1117/12.427783.

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Yang, Yuanlong, Cheng-zhi Jin, Yimin Shen Shen, Paozhong Mao, Yong Xu, and Guoyu Zhou. "Microspectrophotometry of photosensitizer in single living cell of squamous epithelium carcinoma of human tongue." In International Conference on Photodynamic Therapy and Laser Medicine, edited by Junheng Li. SPIE, 1993. http://dx.doi.org/10.1117/12.137036.

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Magunov, Alexander N., and A. Y. Gasilov. "Spatial scanning microspectrophotometer." In Photometry: Selected Papers from the 8th and 9th CIS Conferences, edited by Leonid S. Ushakov. SPIE, 1993. http://dx.doi.org/10.1117/12.166355.

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Gewirtz, A., C. L. shepiro, R. Bovd, and R. W. Colman. "REGULATION OF FACTOR V (FV) EXPRESSION IN HUMAN MEGAKARYOCYTES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643280.

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Abstract:
We have suggested that synthesis of FY by human megakaryocytes (MEGS), is a maturation relrted event. This is based on our finding that when MEGS are cloned from progenitor cells in vitro in FY depleted medium, FY is immunochemically detectable only in morphologically recognizable (mature) cells (Blood 67:1639, '86). The stimuli which induce FY synthesis in MEGS are unknown, but if they are related or linked to factors which regulate terminal maturation processes cellular FY levels might increase as the maturation routine proceeds. To test this hypothesis, mature human MEGS were isolated from normal bone marrow by counterflow centrifugel elutrietion, deposited onto gloss slides by cytocentri-fugation, and then fixed in methanol:acetone. Individual cells were then staged, geometric meon cell diometer (size) determined with on opticol fylor, end FY end DNA levels measured. FV expression in a large area of the MEG (38 urn in diameter) was semi-quantitated by microspectrophotometry (MSPEC) using a sensitive Texas Red labeled streptavidin-biotin detection system and a monoclonal antibody probe (B10) directed against the non-binding FY activation peptide (150,000 kd). Cells were then reacted with Chromomycin A3 to allow for simultaneous DNA quantitation by MSPEC in the same cell. Correlation coefficients (r) and coefficient of determination [r2] were computer generated to discern potential relationships between FY expression, and MEG maturation stage, size or ploidy level in a total of 532 MEGS. Characteristics of the population, and r/r2 vs. MEG FV content ere shown below:r/r2 values did not significantly change when total MEG FY content was measured in 100 additional MEGS. Therefore, MEG FY levels varied independently with cell size and ploidy, and did not appear to correlate with degree of mature MEG maturation as determined by standard critera. In two of four preliminary experiments, low dose (8nM ql2h × 4 doses) tetradecanoyl phorbol acetate augmented both the number of MEGS expressing FV, and the level of FY expressed per individual cell. We conclude from these data that once FV synthesis is initiated, FY synthesis and events relating to final cell size and ploidy development are regulated independently. Our data also suggest that MEG FV synthesis may be regulated in part by the phosphoinositide-protein kinase C system.
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Weng, Chun-Jen, Ken-Yuh Hsu, Yung-Fu Chen, Shian-Wen Chang, Wen-Hao Cho, and Fong-Zhi Chen. "Broadband microspectrophotometer with tablet PC control." In 2012 IEEE International Instrumentation and Measurement Technology Conference (I2MTC). IEEE, 2012. http://dx.doi.org/10.1109/i2mtc.2012.6229131.

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Denisov, Igor G., Anatoly I. Bakhtin, Alexey S. Makarov, and Sergey D. Kozlov. "Wide-range microspectrophotometer for monitoring environmental hydrocarbon pollution." In Nondestructive Evaluation Techniques for Aging Infrastructures & Manufacturing, edited by Walter G. Reuter. SPIE, 1999. http://dx.doi.org/10.1117/12.339948.

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Zeng, Haishan, Calum E. MacAulay, Branko Palcic, and David I. McLean. "Autofluorescence distribution in skin tissue revealed by microspectrophotometer measurements." In OE/LASE'93: Optics, Electro-Optics, & Laser Applications in Science& Engineering, edited by R. Rox Anderson, Lawrence S. Bass, Stanley M. Shapshay, John V. White, and Rodney A. White. SPIE, 1993. http://dx.doi.org/10.1117/12.147020.

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Huang, Xiyong, Michael D. Protheroe, Ahmed M. Al-Jumaily, Sharad P. Paul, Andrew N. Chalmers, and Xiang Fu. "Characterising UV transmission property of red hair using microspectrophotometer." In Translation of Lasers and Biophotonics Technologies and Procedures: Toward the Clinic, edited by Lothar D. Lilge and Carsten M. Philipp. SPIE, 2019. http://dx.doi.org/10.1117/12.2522208.

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Niederwald, Hansjörg, Lothar Deisenroth, and Sebastian Nunnendorf. "Off axis microspectrophotometer for optical coating characterization on complex surfaces." In Optical Systems Design 2005. SPIE, 2005. http://dx.doi.org/10.1117/12.625126.

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