Academic literature on the topic 'Microscopic System'

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Journal articles on the topic "Microscopic System"

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Reffner, John A., and William T. Wihlborg. "FR-IR Molecular Microanalysis System." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 2 (August 12, 1990): 270–71. http://dx.doi.org/10.1017/s0424820100134958.

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The IRμs™ is the first fully integrated system for Fourier transform infrared (FT-IR) microscopy. FT-IR microscopy combines light microscopy for morphological examination with infrared spectroscopy for chemical identification of microscopic samples or domains. Because the IRμs system is a new tool for molecular microanalysis, its optical, mechanical and system design are described to illustrate the state of development of molecular microanalysis. Applications of infrared microspectroscopy are reviewed by Messerschmidt and Harthcock.Infrared spectral analysis of microscopic samples is not a new idea, it dates back to 1949, with the first commercial instrument being offered by Perkin-Elmer Co. Inc. in 1953. These early efforts showed promise but failed the test of practically. It was not until the advances in computer science were applied did infrared microspectroscopy emerge as a useful technique. Microscopes designed as accessories for Fourier transform infrared spectrometers have been commercially available since 1983. These accessory microscopes provide the best means for analytical spectroscopists to analyze microscopic samples, while not interfering with the FT-IR spectrometer’s normal functions.
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ENGELHARDT, Eliasz. "Marcello Malpighi: the nervous system under a microscope." Arquivos de Neuro-Psiquiatria 79, no. 4 (April 2021): 346–49. http://dx.doi.org/10.1590/0004-282x-anp-2020-0309.

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ABSTRACT The longstanding study of gross anatomy experienced a considerable improvement with the advent of the microscope in the early 17th century. The representative personality of this new era certainly was Marcello Malpighi, seen as “founder of microscopic anatomy”. He studied, with a rudimentary compound microscope, numerous tissues and organs of several classes of animals, as well as plants. He described, for the first time, the microscopic structure of the nervous system, identifying in the gray matter of its various levels minute elements he took as “glands”. It should be reminded that the concept of “cell” (and “nerve cell”) was unknown at his time. Many researchers followed, performing microscopic studies, but without better results, and Malpighi’s view was maintained until the beginning of the 19th century, when new histological processing and staining techniques appeared, as well as improved microscopes.
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EBISAWA, Mizue, Yukitoshi OTANI, and Norihiro UMEDA. "Microscopic System for Birefringence Mapping." Journal of the Japan Society for Precision Engineering, Contributed Papers 70, no. 6 (2004): 828–32. http://dx.doi.org/10.2493/jspe.70.828.

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Abbott, Alison. "Microscopic marvels: Seeing the system." Nature 459, no. 7247 (June 2009): 630–31. http://dx.doi.org/10.1038/459630a.

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Oku, Hiromasa, Idaku Ishii, and Masatoshi Ishikawa. "A microscopic visual feedback system." Systems and Computers in Japan 35, no. 13 (2004): 71–79. http://dx.doi.org/10.1002/scj.10056.

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Ryan, John P. "Microscopic Anatomy of the Immune System." Journal of Histotechnology 8, no. 1 (March 1985): 27–29. http://dx.doi.org/10.1179/his.1985.8.1.27.

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Miranda, E. N. "Microscopic description of a nonequilibrium system." European Journal of Physics 26, no. 5 (July 29, 2005): 935–38. http://dx.doi.org/10.1088/0143-0807/26/5/025.

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Hofmann, H. M., and G. M. Hale. "Microscopic calculation of the 4He system." Nuclear Physics A 613, no. 1-2 (January 1997): 69–106. http://dx.doi.org/10.1016/s0375-9474(96)00418-6.

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Song, Changjiang, Na Xing, and Gang Wu. "Microscopic Three-Dimensional Measurement System Design." AASRI Procedia 3 (2012): 540–45. http://dx.doi.org/10.1016/j.aasri.2012.11.085.

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Maekaku, K., and Z. Yoshida. "Hierarchical foliation of one-dimensional Vlasov–Poisson system." Physics of Plasmas 29, no. 8 (August 2022): 082303. http://dx.doi.org/10.1063/5.0089574.

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We elucidate the intermediate of the macroscopic fluid model and the microscopic kinetic model by studying the Poisson algebraic structure of the one-dimensional Vlasov–Poisson system. The water-bag model helps formulating the hierarchy of sub-algebras, which interpolates the gap between the fluid and kinetic models. By analyzing the embedding of the sub-manifold of an intermediate hierarchy in a more microscopic hierarchy, we characterize the microscopic effect as the symmetry breaking pertinent to a macroscopic invariant.
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Dissertations / Theses on the topic "Microscopic System"

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Brougher, Jeremy Adam. "Development of a microscopic moiré interferometry system." Connect to this title online, 2007. http://etd.lib.clemson.edu/documents/1193080056/.

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Cuneo, David J. (David Joseph) 1972. "A system-wide evaluation of a traffic control system using microscopic simulation." Thesis, Massachusetts Institute of Technology, 1998. http://hdl.handle.net/1721.1/10104.

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Sadat, Nabi S. Hasan. "Microscopic origin of magnetism in the Hematite-Ilmenite system." Diss., lmu, 2010. http://nbn-resolving.de/urn:nbn:de:bvb:19-117570.

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Morgan, Daniel J. (Daniel John) 1977. "A microscopic simulation laboratory for advanced public transportation system evaluation." Thesis, Massachusetts Institute of Technology, 2002. http://hdl.handle.net/1721.1/84807.

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Anwar, Zubair. "Enabling microscopic simulators to perform system-level analysis of viscoelastic flows." Thesis, Massachusetts Institute of Technology, 2008. http://hdl.handle.net/1721.1/42943.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2008.
This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Includes bibliographical references (p. 249-261).
State-of-the-art methods for simulating viscoelastic flows couple the conservation equations for mass and momentum with a model from kinetic theory that describes the microstructural state of the polymer. Introduction of appropriate numerical discretization and boundary conditions for these equations leads to a hybrid simulation for studying the dynamic behavior of polymeric liquids in complex geometries. This approach represents a rare example of a successful multiscale solution of a physical problem, as it allows investigation of arbitrary models of kinetic theory. The simulations, however, are not amenable to standard numerical techniques for system-level stability, bifurcation, and control analysis as this requires closed form equations. These simulation either use stochastic descriptions for the polymer microstructure that cannot be reduced to closed form, or involve equations for the evolution of a distribution of polymer conformations, which can only be written in closed form by invoking mathematical closure approximations that can have a significant qualitative impact on the predictive ability of these simulations. The focus of this thesis was to develop a novel numerical method that can enable hybrid simulations to perform system-level analysis of polymeric flows. This numerical approach has been applied directly to kinetic theory models and hybrid simulations to obtain stationary states and associated bifurcations and stability information. The method is general in its applicability in that it treats kinetic theory models and hybrid simulations as black boxes that are then used to obtain system-level information without any modification. The methods developed here are illustrated in a variety of problems.
(cont) Steady state results have been obtained for the non-interacting rigid dumbbell model in steady shear, and for the free-draining bead-spring chain model in both steady shear and uniaxial elongation that are in excellent agreement with previous studies and steady state computed from direct integration. The method is also applied to a hybrid simulation for the pressure-driven flow of non-interacting rigid dumbbells in a planar channel with a linear array of equally spaced cylinders. The computed steady state is in agreement with direct integration and qualitatively matches previous computations with closed models. Bifurcation analysis has been performed for the Doi model at equilibrium with the Onsager excluded volume potential. This analysis agrees with previous studies and accurately predicts the isotropic-nematic transition and turning point for the unstable to stable transition on the prolate solution branch. Bifurcation analysis has also been performed for the Doi model in the weak shear flow limit for the Maier-Saupe excluded volume potential. It is found that stable stationary solutions are lost at a limit point beyond which time-periodic tumbling orbits are the only stable solution. This transition occurs via an infinite period global bifurcation, while the limit point approaches a threshold value as the shear rate approaches zero. This result matches a recently published scaling analysis and demonstrates the ability of the method to provide general bifurcation analysis of kinetic theory models. Stability analysis of the fiber-spinning process for polymeric fluids has also been performed by using a hybrid simulation that couples the one-dimensional conservation equations for mass and momentum with a stochastic description for the configuration fields of the Hookean dumbbell model. The steady-state velocity profiles are in good agreement with previous studies with the Oldroyd-B model.
(cont) The analysis predicts onset of the draw resonance instability via a Hopf bifurcation and subsequent stabilization via second Hopf bifurcation in draw ratio parameter space. This result is in good agreement with experimentally observed behavior during polymer fiber-spinning.
by Zubair Anwar.
Ph.D.
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WANG, JIAN. "MICROSCOPIC STUDY OF PHOSPHOROUS REMOVAL PROCESS IN AN ACTIVATED SLUDGE SYSTEM." University of Cincinnati / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1131120207.

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Linke, Sebastian. "Laser scanning system for microscopic and macroscopic investigations of chemical semiconductor-sensors." Thesis, Queen Mary, University of London, 2011. http://qmro.qmul.ac.uk/xmlui/handle/123456789/8836.

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The aim of this thesis was to develop laterally resolved measurement systems for analysing Metal Insulator Semiconductor (MIS)-based potentiometric chemical sensors. Therefore, the Light-Addressable Potentiometric Sensors (LAPS) principle was used to provide chemical images. Two main system variants were under investigation. The first was a high-resolution so called LAPS-Microscope suitable for life science applications such as the investigation of pH changes of living cells. The second was developed for the analysis of the gas response of alloys on large scale semiconductor samples (25x25 mm2). Therefore, the system was called a LAPS-Macroscope. The LAPS-Microscope resolution depends on the optical focus and semiconductor properties. To reduce the semiconductor dependent resolution, 18 different types of samples were prepared varying the boron and carbon doping in thin silicon films. To analyse the LAPS resolution, different techniques were developed. A resolution improvement down to 3 μm compared to bulk silicon was achieved. A sputter target configuration was developed to produce ternary alloys on semiconductor samples with a continuous gradient in metal concentration. Different analysis methods such as EDX and AES were used to characterise the thin alloy films. Using the LAPS-Macroscope, the gas sensitivity of more than 106 different alloy compositions at a single sample can be investigated. As a result, metal concentration differences down to 0.03% can be distinguished. Typically, the sensor response of 625 different alloy compositions to changes in the hydrogen concentration was investigated within 16 min. The ternary alloy system PdxNiyCo1-x-y was analysed with the LAPS-Macroscope showing that nickel in the range up to 24 atom% reduces the hydrogen sensitivity. There was no significant influence of cobalt in the concentration range tested. Further, poisoning experiments with H2S showed improved behaviour of palladium alloys with nickel in the range of ~5-10 atom%. It was shown that an effective high-throughput method for the characterisation of ternary alloys was established, which is called a Continuous Gradient High Throughput Screening Macroscope CG-HTSM.
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Park, Mi-Kyung Huang Tung-Shi. "Development of microscopic imaging system for rapid detection of salmonella in raw chicken." Auburn, Ala, 2009. http://hdl.handle.net/10415/1856.

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Geiger, Dietrich Horst. "Immunoelectron microscopic characterization of glial intermediate filaments in human gliomas." Thesis, Stellenbosch : University of Stellenbosch, 1993. http://hdl.handle.net/10019.1/3386.

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Thesis (MMed (Biomedical Sciences. Anatomy and Histology))--University of Stellenbosch, 1993.
Glial fibrillary acidic protein (GFAP) is found in varying amounts in the cytoplasm of most normal and neoplastic cells of astroglial origin. Though not glial specific, immunoelectron microscopy has shown that vimentin and GFAP are coexpressed as monomers of glial intermediate filaments. These structures display irreversible assembly and a slow metabolic turnover. Although currently applied as astroglial markers, these intermediate filament proteins may reflect the functional and developmental differentiation status of the cells in which they are expressed. Some authors have tried to apply these aspects as diagnostic parameters for grades of malignancy and anaplasia whilst other workers have indicated variable concentrations of GFAP in different astroglial cell types and entities. Different processing protocols, including the use of epoxy and acrylic resins, omission of osmium tetroxide and variations in concentration and incubation time of primary fixatives, were evaluated to find a compromise between antigen availability and acceptable ultrastructure. Thin sections were labelled on grid for GFAP (Dako A561) and vimentin (Dako M725) by means of the indirect immunogold method. For semi- quantification of relative antigen concentrations, a novel method was devised to calculate the labelling density, percentage heterogeneity of the particle distribution and the surface area investigated. This allowed expression of labelling results as a three figure unit. Standardized post-embedding immunoelectron microscopy was performed on 11 normal and neoplastic human tissue specimens. The tissue was exposed to conventional immersion fixation in glutaraldehyde and osmium tetroxide prior to modified embedding in LR White resin. The validity of these results was verified by correlation with conventional histopathological, immunohistochemical and clinical data obtained for each specimen. The presence of epoxy resin in thin sections was shown to reduce antigen availability to such an extent that very low to negative labelling was encountered. Acrylic LR White resin allowed more acceptable immunodetection, but at the cost of inferior ultrastructure and greater instability of thin sections in the electron beam. This masked the effects of glutaraldehyde fixation on the density of the tissuefixative matrix which included destruction of the vimentin and some GFAP associated epitopes. Although osmium tetroxide was required for acceptable ultrastructure, it reduced the labelling sensitivity by 20% and was responsible for premature curing of acrylic resin during impregnation of tissue. Despite superior resolution gained by electron microscopy and the advantage of semi-quantification of labeling results, the labelling sensitivity of this technique is lesser than that of light microscopical immunohistochemistry. Immunoelectron microscopy confirmed the association between GFAP and glial intermediate filaments in almost all the glial tumours studied, correlating well with GFAP expression in matching specimens demonstrated at light microscopical level. In the absence of intermediate filaments, no positivity for GFAP or vimentin was found in oligodendroglial components of mixed tumours. GFAP positivity in astrocytomas was demonstrated by between 17 and 126 particles / µm2, whilst lower figures were obtained for the glioblastoma (PD = 8) and some of the mixed gliomas (Pd = 6). Rosenthal fibres showed both peripheral and central positive labelling for GFAP, thus providing more evidence for their hypothetical degenerative, astroglial nature. The meningioma studied, was GFAP negative, but produced low density positivity for vimentin. Coexpression of GFAP and vimentin was demonstrated in an astroblastoma and degenerative infant brain tissue, thus supporting the presence of both these proteins development of glial structures. Although sites of likely glial intermediate filament synthesis were found, the antigen availability for vimentin was too low to allow a reliable assessment of specific vimentin localization and determination of the GFAP : vimentin ratio in individual intermediate filaments and/or astroglial fibres. Variations in particle densities (PD) which demonstrated GFAP in the various astroglial entities studied, were considered to be a result of variable technical and tissue processing factors rather than truly significant differences in expression of GFAP in individual intermediate filaments. This lead to the conclusion that the GFAP concentration / glial intermediate filament area is likely to be constant for mature glial intermediate filaments and therefore cannot be used to distinguish between different astroglial cells or entities. Whether each cell has a different number of glial intermediate filaments, has not been established satisfactorily. Following complementary conventional immunohistochemistry and careful orientation of biopsy material, the procedure can be applied to suitable specimens for the electron microscopical localization of high concentrations of aldehyde resistant, cytoplasmic antigens.
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Schmitt, Miriam. "Microscopic description of magnetic model compounds." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-110282.

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Solid state physics comprises many interesting physical phenomena driven by the complex interplay of the crystal structure, magnetic and orbital degrees of freedom, quantum fluctuations and correlation. The discovery of materials which exhibit exotic phenomena like low dimensional magnetism, superconductivity, thermoelectricity or multiferroic behavior leads to various applications which even directly influence our daily live. For such technical applications and the purposive modification of materials, the understanding of the underlying mechanisms in solids is a precondition. Nowadays DFT based band structure programs become broadly available with the possibility to calculate systems with several hundreds of atoms in reasonable time scales and high accuracy using standard computers due to the rapid technical and conceptional development in the last decades. These improvements allow to study physical properties of solids from their crystal structure and support the search for underlying mechanisms of different phenomena from microscopic grounds. This thesis focuses on the theoretical description of low dimensional magnets and intermetallic compounds. We combine DFT based electronic structure and model calculations to develop the magnetic properties of the compounds from microscopic grounds. The developed, intuitive pictures were challenged by model simulations with various experiments, probing microscopic and macroscopic properties, such as thermodynamic measurements, high field magnetization, nuclear magnetic resonance or electron spin resonance experiments. This combined approach allows to investigate the close interplay of the crystal structure and the magnetic properties of complex materials in close collaboration with experimentalists. In turn, the systematic variation of intrinsic parameters by substitution or of extrinsic factors, like magnetic field, temperature or pressure is an efficient way to probe the derived models. Especially pressure allows a continuous change of the crystal structure on a rather large energy scale without the chemical complexity of substitution, thus being an ideal tool to consistently alter the electronic structure in a controlled way. Our theoretical results not only provide reliable descriptions of real materials, exhibiting disorder, partial site occupation and/or strong correlations, but also predict fascinating phenomena upon extreme conditions. In parts this theoretical predictions were already confirmed by own experiments on large scale facilities. Whereas in the first part of this work the main purpose was to develop reliable magnetic models of low dimensional magnets, in the second part we unraveled the underlying mechanism for different phase transitions upon pressure. In more detail, the first part of this thesis is focused on the magnetic ground states of spin 1/2 transition metal compounds which show fascinating phase diagrams with many unusual ground states, including various types of magnetic order, like helical states exhibiting different pitch angles, driven by the intimate interplay of structural details and quantum fluctuations. The exact arrangement and the connection of the magnetically active building blocks within these materials determine the hybridization, orbital occupation, and orbital orientation, this way altering the exchange paths and strengths of magnetic interaction within the system and consequently being crucial for the formation of the respective ground states. The spin 1/2 transition metal compounds, which have been investigated in this work, illustrate the great variety of exciting phenomena fueling the huge interest in this class of materials. We focused on cuprates with magnetically active CuO4 plaquettes, mainly arranged into edge sharing geometries. The influence of structural peculiarities, as distortion, folding, changed bonding angles, substitution or exchanged ligands has been studied with respect to their relevance for the magnetic ground state. Besides the detailed description of the magnetic ground states of selected compounds, we attempted to unravel the origin for the formation of a particular magnetic ground state by deriving general trends and relations for this class of compounds. The details of the treatment of the correlation and influence of structural peculiarities like distortion or the bond angles are evaluated carefully. In the second part of this work we presented the results of joint theoretical and experimental studies for intermetallic compounds, all exhibiting an isostructural phase transition upon pressure. Many different driving forces for such phase transitions are known like quantum fluctuations, valence instabilities or magnetic ordering. The combination of extensive computational studies and high pressure XRD, XAS and XMCD experiments using synchrotron radiation reveals completely different underlying mechanism for the onset of the phase transitions in YCo5, SrFe2As2 and EuPd3Bx. This thesis demonstrates on a series of complex compounds that the combination of ab-initio electronic structure calculations with numerical simulations and with various experimental techniques is an extremely powerful tool for a successful description of the intriguing quantum phenomena in solids. This approach is able to reduce the complex behavior of real materials to simple but appropriate models, this way providing a deep understanding for the underlying mechanisms and an intuitive picture for many phenomena. In addition, the close interaction of theory and experiment stimulates the improvement and refinement of the methods in both areas, pioneering the grounds for more and more precise descriptions. Further pushing the limits of these mighty techniques will not only be a precondition for the success of fundamental research at the frontier between physics and chemistry, but also enables an advanced material design on computational grounds.
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Books on the topic "Microscopic System"

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R, Hudec, ed. The Paraboloid-paraboloid microscopic optical X-ray system: First experience. Ondřejov, Czechoslovakia: Astronomical Institute of the Czechoslovak Academy of Sciences, 1987.

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G, Toner P., ed. Subcellular taxonomy: An ultrastructural classification system with diagnostic applications. Washington: Hemisphere Pub. Corp., 1985.

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Tumours of the nervous system: An ultrastructural atlas. London: Springer-Verlag, 1986.

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P, Liberski P., ed. Light and electron microscopic neuropathology of slow virus disorders. Boca Raton, Fla: CRC Press, 1993.

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Dvorak, Ann M. Diagnostic ultrastructural pathology III: A text-atlas of case studies emphasizing endocrine and hematopoietic systems. Boca Raton: CRC Press, 1995.

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Dvorak, Ann M. Diagnostic ultrastructural pathology II: A text-atlas of case studies with emphasis on respiratory and nervous systems. Boca Raton: CRC Press, 1995.

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Jean-Michel, Vallat, ed. Ultrastructural study of the human diseased peripheral nerve. 2nd ed. New York: Elsevier, 1987.

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deF, Webster Henry, ed. The early development of the neopallial wall and area choroidea in fetal rats: A light and electron microscopic study. Berlin: Springer-Verlag, 1991.

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Chezar, Hank. Underwater microscope system. [Reston, Va.]: U.S. Dept. of the Interior, U.S. Geological Survey, 2001.

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Chezar, Hank. Underwater microscope system. [Reston, Va.]: U.S. Dept. of the Interior, U.S. Geological Survey, 2002.

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Book chapters on the topic "Microscopic System"

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Krstić, R. V. "Integumentary System." In Human Microscopic Anatomy, 447–81. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-662-02676-2_11.

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Krstić, R. V. "Nervous System." In Human Microscopic Anatomy, 483–503. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-662-02676-2_12.

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Krstić, R. V. "Cardiovascular System." In Human Microscopic Anatomy, 39–67. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-662-02676-2_4.

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Krstić, R. V. "Respiratory System." In Human Microscopic Anatomy, 123–59. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-662-02676-2_6.

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Krstić, R. V. "Endocrine System." In Human Microscopic Anatomy, 257–93. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-662-02676-2_8.

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Krstić, R. V. "Lymphatic or Immune System." In Human Microscopic Anatomy, 69–121. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-662-02676-2_5.

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Sinico, Renato Alberto, Filippo Maria Sala, Maria Rosa Pozzi, Paolo Fabbrini, and Federico Pieruzzi. "Microscopic Polyangiitis." In Rare Diseases of the Immune System, 131–44. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-02239-6_8.

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Sator, Nicolas, Nicolas Pavloff, and Lénaïc Couëdel. "Microscopic Description of a Macroscopic System." In Statistical Physics, 1–30. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003272427-1.

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Popp, James A., and Nancy A. Monteiro-Riviere. "Macroscopic, Microscopic, and Ultrastructural Anatomy of the Nasal Cavity, Rat." In Respiratory System, 3–10. Berlin, Heidelberg: Springer Berlin Heidelberg, 1985. http://dx.doi.org/10.1007/978-3-642-96846-4_1.

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Bioulac-Sage, P., J. Saric, and C. Balabaud. "Microscopic Anatomy of the Intrahepatic Circulatory System." In Portal Hypertension, 13–26. Tokyo: Springer Japan, 1991. http://dx.doi.org/10.1007/978-4-431-68361-2_2.

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Conference papers on the topic "Microscopic System"

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Wang, Yuezong, Chao Long, and Yundong Sun. "System design based on microscopic vision with stereo light microscope for gripping microscopic objects." In 2017 IEEE International Conference on Mechatronics and Automation (ICMA). IEEE, 2017. http://dx.doi.org/10.1109/icma.2017.8015962.

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Zhelnov, Vladislav A., Nikita V. Chernomyrdin, Anna S. Kucheryavenko, Irina N. Dolganova, Gleb M. Katyba, and Kirill I. Zaytsev. "Characterizing solid immersion focusing system using numerical modeling." In Advances in Microscopic Imaging, edited by Emmanuel Beaurepaire, Adela Ben-Yakar, and YongKeun Park. SPIE, 2021. http://dx.doi.org/10.1117/12.2615816.

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Lu, Sheng-Huei, and Hong Hua. "Multifunctional Three-dimensional Microscopic System." In 3D Image Acquisition and Display: Technology, Perception and Applications. Washington, D.C.: OSA, 2016. http://dx.doi.org/10.1364/3d.2016.tth2a.2.

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Schneckenburger, Herbert, Petra Weber, Michael Wagner, Sandra Enderle, Julian Weghuber, and Peter Lanzerstorfer. "Combining TIR and FRET: from fluorescence microscopy to a multi-well reader system." In Advances in Microscopic Imaging, edited by Francesco S. Pavone, Emmanuel Beaurepaire, and Peter T. So. SPIE, 2019. http://dx.doi.org/10.1117/12.2526416.

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Aoyama, Tadayoshi, Mamoru Kaneishi, Takeshi Takaki, Idaku Ishii, Sarau Takeno, Masaru Takeuchi, Jun Nakanishi, and Yasuhisa Hasegawa. "Real-time microscopic video shooting using a view-expanded microscope system." In 2017 International Symposium on Micro-NanoMechatronics and Human Science (MHS). IEEE, 2017. http://dx.doi.org/10.1109/mhs.2017.8305257.

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Kim, Minkyung, and Hyun-Joon Shin. "Development of all-optical imaging system for studying cerebral blood flow regulation using optogenetics." In Advances in Microscopic Imaging, edited by Francesco S. Pavone, Emmanuel Beaurepaire, and Peter T. So. SPIE, 2019. http://dx.doi.org/10.1117/12.2534721.

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Lightley, J., F. Gorlitz, S. Kumar, R. Kalita, A. Kolbeinsson, E. Garcia, Y. Alexandrov, et al. "Robust optical autofocus system utilizing neural networks applied to automated multiwell plate STORM microscopy." In Advances in Microscopic Imaging, edited by Emmanuel Beaurepaire, Adela Ben-Yakar, and YongKeun Park. SPIE, 2021. http://dx.doi.org/10.1117/12.2615663.

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Xing, Fangjian, Hongwei Chen, Minghua Chen, Sigang Yang, and Shizhong Xie. "Wavelength division ultrafast microscopic imaging system." In 2013 IEEE International Topical Meeting on Microwave Photonics (MWP 2013). IEEE, 2013. http://dx.doi.org/10.1109/mwp.2013.6724046.

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Nanai, Laszlo, Cs Beleznai, Ferenc Ignacz, and Vince Orova. "Economic microscopic image analysis/processing system." In OPTIKA '98: Fifth Congress on Modern Optics, edited by Gyorgy Akos, Gabor Lupkovics, and Andras Podmaniczky. SPIE, 1998. http://dx.doi.org/10.1117/12.324560.

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Jin, Lu, Binyu Li, Yucong Wu, Shun Wang, Huaxu Tang, and Yueshu Feng. "System design of microscopic ghost imaging." In 3rd International Conference on Laser, Optics and Optoelectronic Technology (LOPET 2023), edited by Xiaotian Li and Manuel Filipe Costa. SPIE, 2023. http://dx.doi.org/10.1117/12.2690358.

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Reports on the topic "Microscopic System"

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Sadot, Einat, Christopher Staiger, and Zvi Kam Weizmann. functional genomic screen for new plant cytoskeletal proteins and the determination of their role in actin mediated functions and guard cells regulation. United States Department of Agriculture, January 2003. http://dx.doi.org/10.32747/2003.7587725.bard.

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The original objectives of the approved proposal were: 1. To construct a YFP fused Arabidopsis cDNA library in a mammalian expression vector. 2. To infect the library into a host fibroblast cell line and to screen for new cytoskeletal associated proteins using an automated microscope. 3. Isolate the new genes. 4. Characterize their role in plants. The project was approved as a feasibility study to allow proof of concept that would entail building the YFP library and picking up a couple of positive clones using the fluorescent screen. We report here on the construction of the YFP library, the development of the automatic microscope, the establishment of the screen and the isolation of positive clones that are plant cDNAs encoding cytoskeleton associated proteins. The rational underling a screen of plant library in fibroblasts is based on the high conservation of the cytoskeleton building blocks, actin and tubulin, between the two kingdoms (80-90% homology at the level of amino acids sequence). In addition, several publications demonstrated the recognition of mammalian cytoskeleton by plant cytoskeletal binding proteins and vice versa. The major achievements described here are: 1. The development of an automated microscope equipped with fast laser auto-focusing for high magnification and a software controlling 6 dimensions; X, Y position, auto focus, time, color, and the distribution and density of the fields acquired. This system is essential for the high throughput screen. 2. The construction of an extremely competent YFP library efficiently cloned (tens of thousands of clones collected, no empty vectors detected) with all inserts oriented 5't03'. These parameters render it well representative of the whole transcriptome and efficient in "in-frame" fusion to YFP. 3. The strategy developed for the screen allowing the isolation of individual positive cDNA clones following three rounds of microscopic scans. The major conclusion accomplished from the work described here is that the concept of using mammalian host cells for fishing new plant cytoskeletal proteins is feasible and that screening system developed is complete for addressing one of the major bottlenecks of the plant cytoskeleton field: the need for high throughput identification of functionally active cytoskeletal proteins. The new identified plant cytoskeletal proteins isolated in the pilot screen and additional new proteins which will be isolated in a comprehensive screen will shed light on cytoskeletal mediated processes playing a major role in cellular activities such as cell division, morphogenesis, and functioning such as chloroplast positioning, pollen tube and root hair elongation and the movement of guard cells. Therefore, in the long run the screen described here has clear agricultural implications.
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Freeman, Stanley, and Russell J. Rodriguez. The Interaction Between Nonpathogenic Mutants of Colletotrichum and Fusarium, and the Plant Host Defense System. United States Department of Agriculture, September 2000. http://dx.doi.org/10.32747/2000.7573069.bard.

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The intent of this proposal was to study the interaction between nonpathogenic mutants of Colletotrichum magna and Fusarium oxysporum, and the cucurbit host defense system. We had shown previously that a nonpathogenic endophytic mutant path- 1 of C. magna, caused no visible disease symptoms but protected watermelon seedlings from disease caused by the wildtype isolate and F. o. niveum. Objectives were: 1) Determine the microscopic, biochemical and molecular genetic interaction between "protected" (path- 1 colonized) cucurbit hosts and wildtype isolates of C. magna; 2) Isolate non-pathogenic mutants of F.o. melonis and test feasibility for protecting plants against fungal diseases. We found that path-1 caused no visible disease symptoms in cucurbit seedlings but conferred disease resistance against pathogenic isolates of C. magna, C. orbiculare, and F. oxysporum. Disease resistance conferred by path-1 correlated to a decrease in the time of activation of host defense systems after exposure of path-1 colonized plants to virulent pathogens. This was determined by monitoring the biochemical activity of PAL and peroxidase, and the deposition of lignin. It appears that path-1-conferred disease resistance is a multigenic phenomenon which should be more difficult for pathogen to overcome than single gene conferred resistance. Based on the benefits conferred by path-1, we have defined this mutant as expressing a mutualistic lifestyle. REMI (restriction enzyme-mediated integration) nonpathogenic mutants were also isolated using pHA1.3 plasmid linearized with Hind III and transformed into wildtype C. magna. The integrated vector and flanking genomic DNA sequences in REMI mutant R1 was re-isolated and cloned resulting in a product of approximately 11 kb designated pGMR1. Transformations of wildtype C. magna with pGMR1 resulted in the same non-pathogenic phenotype. A nonpathogenic mutant of F.o. melonis (pathogenic to melon) was isolated that colonized melon plants but elicited no disease symptoms in seedlings and conferred 25 - 50% disease protection against the virulent wildtype isolate. Subsequently, nonpathogenic mutant isolates of F.o. niveum (pathogenic to watermelon) were also isolated. Their protection capacity against the respective wildtype parent is currently under investigation. This research has provided information toward a better understanding of host-parasite interactions; specifically, endophytes, pathogens and their hosts. It will also allow us to assess the potential for utilizing nonpathogenic mutants as biological control agents against fungal pathogens and isolating molecular genetic factors of pathogenicity in Fusarium.
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Girard, Gerald, and David Enos. Differential imaging microscope system acquisition software reference. Office of Scientific and Technical Information (OSTI), September 2013. http://dx.doi.org/10.2172/1104704.

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Enikov, Eniko T. Multimode Scanning Probe Microscope System for Nanocomposite Actuators. Fort Belvoir, VA: Defense Technical Information Center, July 2002. http://dx.doi.org/10.21236/ada406940.

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Mohammadi, N., D. Corrigan, A. A. Sappin, and N. Rayner. Evidence for a Neoarchean to earliest-Paleoproterozoic mantle metasomatic event prior to formation of the Mesoproterozoic-age Strange Lake REE deposit, Newfoundland and Labrador, and Quebec, Canada. Natural Resources Canada/CMSS/Information Management, 2022. http://dx.doi.org/10.4095/330866.

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A complete suite of bulk major- and trace-elements measurements combined with macroscopic/microscopic observations and mineralogy guided by scanning electron microscope-energy dispersive spectrometry (SEM-EDS) analyses were applied on Nekuashu (2.55 Ga) and Pelland (2.32 Ga) intrusions in northern Canada, near the Strange Lake rare earth elements (REE) deposit, to evaluate their magmatic evolution and possible relations to the Mesoproterozoic Strange Lake Peralkaline Complex (SLPC). These Neoarchean to earliest-Paleoproterozoic intrusions, part of the Core Zone in southeastern Churchill Province, comprise mainly hypersolvus suites, including hornblendite, gabbro, monzogabbro/monzodiorite, monzonite, syenite/augite-syenite, granodiorite, and mafic diabase/dyke. However, the linkage of the suites and their petrogenesis are poorly understood. Geochemical evidence suggests a combination of 'intra-crustal multi-stage differentiation', mainly controlled by fractional crystallization (to generate mafic to felsic suites), and 'accumulation' (to form hornblendite suite) was involved in the evolution history of this system. Our model proposes that hornblendite and mafic to felsic intrusive rocks of both intrusions share a similar basaltic parent magma, generated from melting of a hydrous metasomatized mantle source that triggered an initial REE and incompatible element enrichment that prepared the ground for the subsequent enrichment in the SLPC. Geochemical signature of the hornblendite suite is consistent with a cumulate origin and its formation during the early stages of the magma evolution, however, the remaining suites were mainly controlled by 'continued fractional crystallization' processes, producing more evolved suites: gabbronorite/hornblende-gabbro ? monzogabbro/monzodiorite ? monzonite ? syenite/augite-syenite. In this proposed model, the hydrous mantle-derived basaltic magma was partly solidified to form the mafic suites (gabbronorite/hornblende-gabbro) by early-stage plagioclase-pyroxene-amphibole fractionation in the deep crust while settling of the early crystallized hornblende (+pyroxene) led to the formation of the hornblendite cumulates. The subsequent fractionation of plagioclase, pyroxene, and amphibole from the residual melt produced the more intermediate suites of monzogabbro/monzodiorite. The evolved magma ascended upward into the shallow crust to form monzonite by K-feldspar fractionation. The residual melt then intruded at shallower depth to form syenite/augite-syenite with abundant microcline crystals. The granodiorite suite was probably generated from lower crustal melts associated with the mafic end members. Later mafic diabase/dykes were likely generated by further partial melting of the same source at depth that were injected into the other suites.
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Jiang, Qing. Investigation of Microscopic Mechanisms of Failure of Electronic Smart Materials/Systems. Fort Belvoir, VA: Defense Technical Information Center, September 1994. http://dx.doi.org/10.21236/ada284830.

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Larbalestier, David C. Superconducting Magnet System for a Low Temperature Laser Scanning Microscope. Fort Belvoir, VA: Defense Technical Information Center, September 2006. http://dx.doi.org/10.21236/ada461018.

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Elbaum, Michael, and Peter J. Christie. Type IV Secretion System of Agrobacterium tumefaciens: Components and Structures. United States Department of Agriculture, March 2013. http://dx.doi.org/10.32747/2013.7699848.bard.

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Objectives: The overall goal of the project was to build an ultrastructural model of the Agrobacterium tumefaciens type IV secretion system (T4SS) based on electron microscopy, genetics, and immunolocalization of its components. There were four original aims: Aim 1: Define the contributions of contact-dependent and -independent plant signals to formation of novel morphological changes at the A. tumefaciens polar membrane. Aim 2: Genetic basis for morphological changes at the A. tumefaciens polar membrane. Aim 3: Immuno-localization of VirB proteins Aim 4: Structural definition of the substrate translocation route. There were no major revisions to the aims, and the work focused on the above questions. Background: Agrobacterium presents a unique example of inter-kingdom gene transfer. The process involves cell to cell transfer of both protein and DNA substrates via a contact-dependent mechanism akin to bacterial conjugation. Transfer is mediated by a T4SS. Intensive study of the Agrobacterium T4SS has made it an archetypal model for the genetics and biochemistry. The channel is assembled from eleven protein components encoded on the B operon in the virulence region of the tumor-inducing plasmid, plus an additional coupling protein, VirD4. During the course of our project two structural studies were published presenting X-ray crystallography and three-dimensional reconstruction from electron microscopy of a core complex of the channel assembled in vitro from homologous proteins of E. coli, representing VirB7, VirB9, and VirB10. Another study was published claiming that the secretion channels in Agrobacterium appear on helical arrays around the membrane perimeter and along the entire length of the bacterium. Helical arrangements in bacterial membranes have since fallen from favor however, and that finding was partially retracted in a second publication. Overall, the localization of the T4SS within the bacterial membranes remains enigmatic in the literature, and we believe that our results from this project make a significant advance. Summary of achievements : We found that polar inflations and other membrane disturbances relate to the activation conditions rather than to virulence protein expression. Activation requires low pH and nutrient-poor medium. These stress conditions are also reflected in DNA condensation to varying degrees. Nonetheless, they must be considered in modeling the T4SS as they represent the relevant conditions for its expression and activity. We identified the T4SS core component VirB7 at native expression levels using state of the art super-resolution light microscopy. This marker of the secretion system was found almost exclusively at the cell poles, and typically one pole. Immuno-electron microscopy identified the protein at the inner membrane, rather than at bridges across the inner and outer membranes. This suggests a rare or transient assembly of the secretion-competent channel, or alternatively a two-step secretion involving an intermediate step in the periplasmic space. We followed the expression of the major secreted effector, VirE2. This is a single-stranded DNA binding protein that forms a capsid around the transferred oligonucleotide, adapting the bacterial conjugation to the eukaryotic host. We found that over-expressed VirE2 forms filamentous complexes in the bacterial cytoplasm that could be observed both by conventional fluorescence microscopy and by correlative electron cryo-tomography. Using a non-retentive mutant we observed secretion of VirE2 from bacterial poles. We labeled the secreted substrates in vivo in order detect their secretion and appearance in the plant cells. However the low transfer efficiency and significant background signal have so far hampered this approach.
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Madey, Theodore E. A Proposal to Acquire a Variable Temperature Scanning Tunneling Microscope System. Fort Belvoir, VA: Defense Technical Information Center, July 2001. http://dx.doi.org/10.21236/ada396338.

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Kadakia, Madhavi P. Optical Inverted Microscope Imaging System for Biological and Non-Biological Samples. Fort Belvoir, VA: Defense Technical Information Center, February 2009. http://dx.doi.org/10.21236/ada499962.

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