Academic literature on the topic 'Microscope'

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Journal articles on the topic "Microscope"

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J. H., Youngblom, Wilkinson J., and Youngblom J.J. "Telepresence Confocal Microscopy." Microscopy and Microanalysis 6, S2 (August 2000): 1164–65. http://dx.doi.org/10.1017/s1431927600038319.

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The advent of the Internet has allowed the development of remote access capabilities to a growing variety of microscopy systems. The Materials MicroCharacterization Collaboratory, for example, has developed an impressive facility that provides remote access to a number of highly sophisticated microscopy and microanalysis instruments. While certain types of microscopes, such as scanning electron microscopes, transmission electron microscopes, scanning probe microscopes, and others have already been established for telepresence microscopy, no one has yet reported on the development of similar capabilities for the confocal laser scanning microscope.At California State University-Stanislaus, home of the CSUPERB (California State University Program for Education and Research in Biotechnology) Confocal Microscope Core Facility, we have established a remote access confocal laser scanning microscope facility that allows users with virtually any type of computer platform to connect to our system. Our Leica TCS NT confocal system, with an interchangeable upright (DMRXE) and inverted microscope (DMIRBE) set up,
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O'Keefe, Michael A., John H. Turner, John A. Musante, Crispin J. D. Hetherington, A. G. Cullis, Bridget Carragher, Ron Jenkins, et al. "Laboratory Design for High-Performance Electron Microscopy." Microscopy Today 12, no. 3 (May 2004): 8–17. http://dx.doi.org/10.1017/s1551929500052093.

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Since publication of the classic text on the electron microscope laboratory by Anderson, the proliferation of microscopes with field emission guns, imaging filters and hardware spherical aberration correctors (giving higher spatial and energy resolution) has resulted in the need to construct special laboratories. As resolutions iinprovel transmission electron microscopes (TEMs) and scanning transmission electron microscopes (STEMs) become more sensitive to ambient conditions. State-of-the-art electron microscopes require state-of-the-art environments, and this means careful design and implementation of microscope sites, from the microscope room to the building that surrounds it. Laboratories have been constructed to house high-sensitive instruments with resolutions ranging down to sub-Angstrom levels; we present the various design philosophies used for some of these laboratories and our experiences with them. Four facilities are described: the National Center for Electron Microscopy OAM Laboratory at LBNL; the FEGTEM Facility at the University of Sheffield; the Center for Integrative Molecular Biosciences at TSRI; and the Advanced Microscopy Laboratory at ORNL.
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Sutriyono, Widodo, and Retno Suryandari. "Addition of Illuminator Fiber Optic to Produce 3 Dimension Effects in Micrographic Observation Using Upright Microscope." Proceeding International Conference on Science and Engineering 3 (April 30, 2020): 493–96. http://dx.doi.org/10.14421/icse.v3.551.

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Microscope is one of the tools used in practicums with high intensity. The use of a microscope adjusts to the object to be observed in order to obtain optimal micrographic results. Stereo microscopes are used to observe three-dimensional objects. Upright microscopes are used to observe two-dimensional objects. This study aims to combine the two advantages of stereo microscopy that can produce three-dimensional micrography with the advantages of an upright microscope that has a high total magnification. The method used in this study is an experimental method by adding an optical fiber illuminator in the use of an upright microscope and then applying it in various observations. The conclusion of this research is the addition of an optical fiber illuminator in observations using an upright microscope can replace the function of a stereo microscope; can produce three-dimensional effects and increase magnification in Daphnia magna micrographic observations.
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Davidson, Michael W. "50 Most Frequently Asked Questions About Optical Microscopy." Microscopy Today 8, no. 6 (August 2000): 12–19. http://dx.doi.org/10.1017/s1551929500052780.

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A significant percentage of technical experts who employ optical microscopes have had little or no formal training in optical microscope basics. Some, typically, were required to use microscopes during their technical education but, in general, microscope terminology and technology was a sideline to their major training. As a result, many useful basic microscope technical details were not learned because they were not necessary to accomplish what was needed in order to survive their major class work. At Florida State University, we try to make the [earning of microscope technology an inherent part of the students training. An important part of this training is this compendium of 50 of the most frequently asked questions about Optical Microscopy.
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Johnson, W. Travis. "Advantages of Simultaneous Imaging Using an Atomic Force Microscope Integrated with an Inverted Light Microscope." Microscopy Today 19, no. 6 (October 28, 2011): 22–29. http://dx.doi.org/10.1017/s1551929511001222.

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Atomic Force Microscopy (AFM) permits measurements on biological samples below the limits of light microscopy resolution under physiological environments and other controlled conditions. Consequently, AFM has become an increasingly valuable technique in cell biology. One of the most exciting advances in AFM instrumentation has been its integration with the light microscope. This permits investigators to take advantage of the power and utility of light microscopy and scanning probe microscopy simultaneously. In combining a light microscope with an AFM, scanner components must be specifically designed so that they do not adversely impact the light microscope's optical imaging capabilities. For example, an AFM-ILM (inverted light microscope) hybrid system should be fully compatible with the highest quality, off-the-shelf 0.50–0.55 NA numerical aperture (NA) OEM objectives and condensers.
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Youngblom, J. H., J. Wilkinson, and J. J. Youngblom. "Confocal Laser Scanning Microscopy By Remote Access." Microscopy Today 7, no. 7 (September 1999): 32–33. http://dx.doi.org/10.1017/s1551929500064798.

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In recent years there have been a growing number of facilities interested in developing remote access capabilities to a variety of microscopy systems. While certain types of microscopes, such as electron microscopes and scanning probe microscopes have been well established for telepresence microscopy, no one has yet reported on the development of similar capabilities for the confocal microscope.At California State University, home to the CSUPERB (California State University Program for Education and Research in Biotechnology) Confocal Microscope Core Facility, we have established a remote access confocal laser scanning microscope facility that allows users with virtually any type of computer platform to connect to our system.
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Graef, M. De, N. T. Nuhfer, and N. J. Cleary. "Implementation Of A Digital Microscopy Teaching Environment." Microscopy and Microanalysis 5, S2 (August 1999): 4–5. http://dx.doi.org/10.1017/s1431927600013349.

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The steady evolution of computer controlled electron microscopes is dramatically changing the way we teach microscopy. For today’s microscopy student, an electron microscope may be just another program on the desktop of whatever computer platform he or she uses. This is reflected in the use of the term Desktop Microscopy. The SEM in particular has become a mouse and keyboard controlled machine, and running the microscope is not very different from using a drawing program or a word processor. Transmission electron microscopes are headed in the same direction.While one can debate whether or not it is wise to treat an SEM or a TEM as just another black-box computer program, it is a fact that these machines are here to stay, which means that microscopy educators must adapt their traditional didactic tools and methods. One way to bring electron microscopes into the classroom is through the use of remote control software packages, such as Timbuktu Pro or PC-Anywhere. The remote user essentially opens a window containing the desktop of the microscope control computer and has all functions available. On microscopes with specialized graphics boards, integration of the image and control display for remote operation may not be straightforward, and often requires the purchase of additional graphics boards for the remote machine.
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Mao, Hong, Robin Diekmann, Hai Po H. Liang, Victoria C. Cogger, David G. Le Couteur, Glen P. Lockwood, Nicholas J. Hunt, et al. "Cost-efficient nanoscopy reveals nanoscale architecture of liver cells and platelets." Nanophotonics 8, no. 7 (July 9, 2019): 1299–313. http://dx.doi.org/10.1515/nanoph-2019-0066.

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AbstractSingle-molecule localization microscopy (SMLM) provides a powerful toolkit to specifically resolve intracellular structures on the nanometer scale, even approaching resolution classically reserved for electron microscopy (EM). Although instruments for SMLM are technically simple to implement, researchers tend to stick to commercial microscopes for SMLM implementations. Here we report the construction and use of a “custom-built” multi-color channel SMLM system to study liver sinusoidal endothelial cells (LSECs) and platelets, which costs significantly less than a commercial system. This microscope allows the introduction of highly affordable and low-maintenance SMLM hardware and methods to laboratories that, for example, lack access to core facilities housing high-end commercial microscopes for SMLM and EM. Using our custom-built microscope and freely available software from image acquisition to analysis, we image LSECs and platelets with lateral resolution down to about 50 nm. Furthermore, we use this microscope to examine the effect of drugs and toxins on cellular morphology.
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Madrid-Wolff, Jorge, and Manu Forero-Shelton. "Protocol for the Design and Assembly of a Light Sheet Light Field Microscope." Methods and Protocols 2, no. 3 (July 4, 2019): 56. http://dx.doi.org/10.3390/mps2030056.

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Light field microscopy is a recent development that makes it possible to obtain images of volumes with a single camera exposure, enabling studies of fast processes such as neural activity in zebrafish brains at high temporal resolution, at the expense of spatial resolution. Light sheet microscopy is also a recent method that reduces illumination intensity while increasing the signal-to-noise ratio with respect to confocal microscopes. While faster and gentler to samples than confocals for a similar resolution, light sheet microscopy is still slower than light field microscopy since it must collect volume slices sequentially. Nonetheless, the combination of the two methods, i.e., light field microscopes that have light sheet illumination, can help to improve the signal-to-noise ratio of light field microscopes and potentially improve their resolution. Building these microscopes requires much expertise, and the resources for doing so are limited. Here, we present a protocol to build a light field microscope with light sheet illumination. This protocol is also useful to build a light sheet microscope.
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Kersker, M., C. Nielsen, H. Otsuji, T. Miyokawa, and S. Nakagawa. "The JSM-890 ultra high resolution Scanning Electron Microscope." Proceedings, annual meeting, Electron Microscopy Society of America 47 (August 6, 1989): 88–89. http://dx.doi.org/10.1017/s0424820100152410.

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Historically, ultra high spatial resolution electron microscopy has belonged to the transmission electron microscope. Today, however, ultra high resolution scanning electron microscopes are beginning to challenge the transmission microscope for the highest resolution.To accomplish high resolution surface imaging, not only is high resolution required. It is also necessary that the integrity of the specimen be preserved, i.e., that morphological changes to the specimen during observation are prevented. The two major artifacts introduced during observation are contamination and beam damage, both created by the small, high current-density probes necessary for high signal generation in the scanning instrument. The JSM-890 Ultra High Resolution Scanning Microscope provides the highest resolution probe attainable in a dedicated scanning electron microscope and its design also accounts for the problematical artifacts described above.Extensive experience with scanning transmission electron microscopes lead to the design considerations of the ultra high resolution JSM- 890.
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Dissertations / Theses on the topic "Microscope"

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Payton, Oliver David. "High-speed atomic force microscopy under the microscope." Thesis, University of Bristol, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.574416.

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SINCE its invention in 1986, the atomic force microscope (AFM) has revolutionised the field of nanotechnology and nanoscience. It is a tool that has enabled research into areas of medicine, advanced materials, biology, chemistry and physics. However due to its low frame rate it is a tool that has been limited to imaging small areas using a time lapse technique. It has only been in recent years that the frame rate of the device has been increased in a tool known as high-speed AFM (HSAFM). This increased frame rate allows, for the first time, biological processes to be viewed in real time or macro sized areas to be imaged with nanoscale resolution. The research presented here concentrates on a specific type of high-speed AFM developed at the University of Bristol called contact mode HSAFM. This thesis explains how the microscope is able to function, and presents a leap in image quality due to an increased understanding of the dynamics of the system. The future of the device is also discussed. III
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Franklin, Thomas. "Scanning ionoluminescence microscopy with a helium ion microscope." Thesis, University of Southampton, 2012. https://eprints.soton.ac.uk/352281/.

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The ORIONR PLUS scanning helium ion microscope (HIM) images at sub nanometer resolution. Images of the secondary electron emission have superior resolution and depth of field compared to a scanning electron microscope (SEM). Ionoluminescent imaging is not an area that has been extensively explored by typical ion beam systems as they have large spot sizes in the region of microns, leading to poor spatial resolution. This thesis confirms that the ORIONR PLUS can form images from the ionoluminescent signal, resolutions of 20nm can be obtained for images of bright nanoparticles. Ionoluminescence spectra can also be obtained from some samples. The position of emission peaks in samples under the ORIONR PLUS does not deviate significantly from cathodoluminescence (CL) peaks under SEM. However, the relative heights of the emission peaks in a sample can vary between ionoluminescence (IL) and CL. In addition, It is found that there exists a proportional relationship between acceleration voltage and ionoluminescent signal in the ORIONR PLUS, this relationship is also exhibited in CL. However, when normalised for current and acceleration voltage there appears to be no samples that show greater luminescence under ionoluminescence than cathodoluminescence, with ionoluminescent intensities up to an order of magnitude lower. Ionoluminescence under the ORIONR PLUS is found to be a poor candidate for the analysis of direct band gap semiconductors, this is attributed to the smaller interaction volumes and achievable beam current of the ORIONR PLUS. It is also found that some direct band gap materials are very susceptible to beam damage under the ion beam at beam doses typically used for secondary electron (SE) imaging. It is possible to obtain simultaneous IL and SE images of organic fluorospores in a biological sample. However, the luminescence of the fluorospores was only just sufficient to form images with a 200nm resolution. Rare earth based nanoparticles show brighter luminescence and greater resistance to beam damage than organic fluorospores. If such particles could be utilised for immunofluorescence it would make combined secondary electron and immunofluorescence imaging under the ORIONR PLUS a viable technique.
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Baida, Fadi Issam. "Microscopie hybride : association d'un microscope optique en champ proche et d'un microscope à forces atomiques : principe et réalisation." Besançon, 1995. http://www.theses.fr/1995BESA2017.

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Ce travail de recherche se situe dans la dynamique des microscopies dites en "champ proche" ou à sondes locales. La résolution de ces microscopies dépend des effets physiques liés à la géométrie de la sonde et au type d'interaction de cette dernière avec l'échantillon à analyser. Dans ce contexte, nous avons développé, réalisé et exploité un instrument combinant deux détections : l'une optique et l'autre à forces atomiques. La première permet de déterminer la distribution du champ lumineux au voisinage immédiat de l'objet tandis que la détection de force fournit la topographie du même site de l'objet. La double détection a été démontrée. A partir de ces deux informations, il est possible de mieux comprendre les phénomènes d'interaction matière-rayonnement à l'échelle nanométrique et de mettre en évidence les effets optiques directement liés soit à la topographie soit à la nature physico-chimique de l'objet. Deux essais d'optimisation des images optiques ont été développés, soit par métallisation de la pointe, soit par l'exploitation de l'interférométrie optique en champ proche. Le dernier chapitre est une modélisation des microscopes optiques en champ proche basée sur un calcul perturbatif au premier ordre permettant de calculer le champ électromagnétique diffracté par l'ensemble sonde-objet (méthode couplée)
This work deals with near field or local probe microscopes. The resolution of such microscopes depends on the physical effects connected to the geometry of the probe and onthe type of the interaction between the probe with the sample under test. In. This framework, we have developed and realized an instrument combining both an optical detection and an atomic force detection. The first one allows us to determine the mapping of the light intensity on the object surface, whereas the force detection provides the topography on the same site of the object. The double detection has been realized ansd demonstrated. The acquisition of these complemental data allows us to understand the interaction between light and matter at nanometer scale and to point out the optical effects -connected either to the topography or to the physical - chemical properties of the object. Two attempts of optical image optimization have been carried out either by metallization of the tip or by using optical resonance in a Fabry-Pérot cavity. The last chapter deals with a modeling based on a first order perturbative computation ( coupled method)
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Szelc, Jedrzej. "THz imaging and microscopy : a multiplexed near-field TeraHertz microscope." Thesis, University of Southampton, 2011. https://eprints.soton.ac.uk/209643/.

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Bethge, Philipp. "Development of a two-photon excitation STED microscope and its application to neuroscience." Thesis, Bordeaux, 2014. http://www.theses.fr/2014BORD0018/document.

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L’avènement de la microscopie STED (Stimulated Emission Depletion) a bouleversé le domaine desneurosciences du au fait que beaucoup de structures neuronale, tels que les épines dendritiques, lesaxones ou les processus astrocytaires, ne peuvent pas être correctement résolu en microscopiephotonique classique. La microscopie 2-photon est une technique d’imagerie photonique très largement utilisée dans le domaine des neurosciences car elle permet d’imager les événements dynamique en profondeur dans le tissu cérébral, offrant un excellent sectionnement optique et une meilleure profondeur de pénétration. Cependant, la résolution spatiale de cette approche est limitée autour de 0.5 μm, la rendant inappropriée pour étudier les détails morphologiques des neurones et synapses. Le but de mon travail de thèse était à A) développer un microscope qui permet d'améliorer l'imagerie 2-photon en la combinant avec la microscopie STED et B) démontrer son potentiel pour l'imagerie à l'échelle nanométrique de processus neuronaux dynamiques dans des tranches de cerveau aigus et in vivo. Le nouveau microscope permet d'obtenir une résolution spatiale latérale de ~ 50 nm à des profondeurs d'imagerie de ~ 50 μm dans du tissu cérébral vivant. Il fonctionne avec des fluorophores verts, y compris les protéines fluorescentes communes telles que la GFP et YFP, offrant le contraste de deux couleurs basé sur la détection spectrale et linéaire ‘unmixing’. S’agissant d’un microscope droit, utilisant un objectif à immersion ayant une grande distance de travail, nous avons pu incorporer des techniques électrophysiologiques comme patch-clamp et ajouter une plateforme pour l'imagerie in vivo. J’ai utilise ce nouveau microscope pour imager des processus neuronaux fins et leur dynamique à l’échelle nanométrique dans différent types de préparations et des régions différentes du cerveau. J’ai pu révéler des nouvelles caractéristiques morphologique des dendrites et épines. En outre, j'ai exploré différentes stratégies de marquage pour pouvoir utiliser la microscopie STED pour imager le trafic des protéines et de leur dynamique à l'échelle nanométrique dans des tranches de cerveau
The advent of STED microscopy has created a lot of excitement in the field of neuroscience becausemany important neuronal structures, such as dendritic spines, axonal shafts or astroglial processes,cannot be properly resolved by regular light microscopy techniques. Two-photon fluorescence microscopy is a widely used imaging technique in neuroscience because it permits imaging dynamic events deep inside light-scattering brain tissue, providing high optical sectioning and depth penetration. However, the spatial resolution of this approach is limited to around half a micron, and hence is inadequate for revealing many morphological details of neurons and synapses. The aim of my PhD work was to A) develop a microscope that improves on two-photon imaging by combining it with STED microscopy and to B) demonstrate its potential for nanoscale imaging of dynamic neural processes in acute brain slices and in vivo. The new microscope achieves a lateral spatial resolution of ~50 nm at imaging depths of ~50 μm in living brain slices. It works with green fluorophores, including common fluorescent proteins like GFP and YFP, offering two-color contrast based on spectral detection and linear unmixing. Because of its upright design using a long working distance water-immersion objective, it was possible to incorporate electrophysiological techniques like patch-clamping or to add a stage for in vivo imaging. I have used the new microscope to image fine neural processes and their nanoscale dynamics in different experimental preparations and brain regions, revealing new and interesting morphological features of dendrites and spines. In addition, I have explored different labeling strategies to be able to use STED microscopy for visualizing protein trafficking and dynamics at the nanoscale in brain slices
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Battistella, Eliana. "Towards an improved photonic force microscope: a novel technique for biological microscopy." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2017. http://amslaurea.unibo.it/14864/.

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Una delle tecniche più note nello studio topografico di campioni biologici è l’AFM. Ci sono però limitazioni dovute alla presenza del cantilever, il quale pone un limite nella forza minima applicabile su un campione per ottenere un’immagine topografica. Questa forza (ordine dei 10 pN) può essere sufficiente a danneggiare il campione e a deformare i dettagli topografici che si vorrebbero evidenziare. Per superare questo problema si può usare un Photonic Force Microscope, dove il cantilever è sostituito da Optical Tweezers. Questa tecnica permette di effettuare scansioni di campioni biologici applicando forze dell’ordine dei 100 fN. All’interno della trappola ottica viene posizionata una microparticella che agisce da sonda, attraverso la quale possono essere rilevati dettagli topografici del campione. La differenza rispetto al PFM tradizionale si trova proprio nel tipo di sonda utilizzata durante la scansione. Lo standard prevede l’utilizzo di una sonda sferica, di dimensioni dell’ordine dei 100 nm mentre l’ipotesi è che si possano utilizzare delle sonde cilindriche con alla base un dettaglio acuminato che richiama la punta dell’AFM. Questo tipo di sonda consentirebbe di raggiungere una risoluzione maggiore, rispetto al PFM tradizionale, che risente del limite dato dal diametro della sfera. Due differenti setup per la PFM sono stati costruiti e testati durante questo periodo di tesi. Sono state testate diverse microparticelle cilindriche, di dimensioni differenti in termini di aspect ratio con lo scopo di osservare la stabilità di questo tipo di sonda. Nei risultati viene proposto un metodo per controllare la stabilità e l’orientazione della microparticella cilindrica all’interno della trappola ottica. Viene inoltre fatta un’ipotesi su un metodo per stimare la lunghezza della punta che dovrà essere verificata da una misura sistematica. I risultati preliminari riguardanti la scansione di strutture note suggeriscono la validità dell’uso di questo nuovo tipo di sonda.
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Setiawan, Widagdo. "Fermi Gas Microscope." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10225.

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Recent advances in using microscopes in ultracold atom experiment have allowed experimenters for the first time to directly observe and manipulate individual atoms in individual lattice sites. This technique enhances our capability to simulate strongly correlated systems such as Mott insulator and high temperature superconductivity. Currently, all ultracold atom experiments with high resolution imaging capability use bosonic atoms. In this thesis, I present our progress towards creating the fermionic version of the microscope experiment which is more suitable for simulating real condensed matter systems. Lithium is ideal due to the existence of both fermionic and bosonic isotopes, its light mass, which means faster experiment time scales that suppresses many sources of technical noise, and also due to the existence of a broad Feshbach resonance, which can be used to tune the inter-particle interaction strength over a wide range from attractive, non-interacting, and repulsive interactions. A high numerical aperture objective will be used to image and manipulate the atoms with single lattice site resolution. This setup should allow us to implement the Hubbard hamiltonian which could describe interesting quantum phases such as antiferromagnetism, d-wave superfluidity, and high temperature superconductivity. I will also discuss the feasibility of the Raman sideband cooling method for cooling the atoms during the imaging process. We have also developed a new electronic control system to control the sequence of the experiment. This electronic system is very scalable in order to keep up with the increasing complexity of atomic physics experiments. Furthermore, the system is also designed to be more precise in order to keep up with the faster time scale of lithium experiment.
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Le, Falher Eric. "Le microscope conoscopique." Paris, ENST, 1992. http://www.theses.fr/1993ENST0017.

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Le but de cette these etait la comprehension, l'etude et le developpement d'un microscope conoscopique. Au cours de ce travail nous avons developpe une approche de l'holographie conoscopique fondee sur l'optique physique (methode des ondes). Nous avons developpe le calcul de la fonction de transfert optique tridimensionnelle d'un systeme conoscopique. Nous avons etendu le concept de fonction de transfert tridimensionnelle optique au cas d'un systeme opto-informatique. Cette fonction de transfert tridimensionnelle optique servira d'outil general de modelisation des systemes conoscopiques. Dans la partie pratique de ce travail nous avons realise un microscope conoscopique. Ce microscope a necessite une etude de dimensionnement, le test et la mise en uvre de composants optiques, electroniques et informatiques. Nous avons bati l'ensemble des logiciels necessaires a l'exploitation du microscope. De plus nous avons quantifie l'influence des differents parametres physiques et technologiques (quantite de lumiere, stabilite de la mesure, precision, repetabilite) sur ce systeme. Enfin nous avons teste ce microscope sur differents types de materiaux (verre, plaquette de silicium, plaquette d'inp) et obtenu des mesures de surfaces satisfaisantes
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Le, Falher Eric. "Le microscope conoscopique /." Paris : École nationale supérieure des télécommunications, 1993. http://catalogue.bnf.fr/ark:/12148/cb356172863.

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Siebers, Ellen Mary. "Telescope or microscope." Thesis, University of Iowa, 2012. https://ir.uiowa.edu/etd/2987.

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Books on the topic "Microscope"

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Thomas, Mulvey, and Sheppard C. J. R, eds. Advances inoptical and electron microscopy. London: Academic, 1990.

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The principles and practice of electron microscopy. Cambridge [Cambridgeshire]: Cambridge University Press, 1985.

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The principles and practice of electron microscopy. 2nd ed. Cambridge: Cambridge University Press, 1997.

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Bradbury, Savile. An introduction to the optical microscope. Oxford: Bios, 1994.

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Bradbury, Savile. An introduction to the optical microscope. Oxford: Oxford University Press, 1988.

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Burgess, Jeremy. The magnified world. Vero Beach, FL: Rourke Enterprises, 1988.

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Leslie, Johnstone, and Sovka David ill, eds. The microscope book. New York: Sterling Pub. Co., 1996.

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Nachtigall, Werner. Exploring with the microscope. New York: Sterling Pub., 1995.

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Kelly, Jerry, 1955- book designer and Grolier Club, eds. Through a glass clearly: The history & science of the microscope. New York: The Grolier Club, 2013.

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The light microscope: Its use and development. Oxford: Senecio, 1993.

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Book chapters on the topic "Microscope"

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Maddalena, Laura, Paolo Pozzi, Nicolò G. Ceffa, Bas van der Hoeven, and Elizabeth C. Carroll. "Optogenetics and Light-Sheet Microscopy." In Neuromethods, 231–61. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2764-8_8.

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AbstractLight-sheet microscopy is a powerful method for imaging small translucent samples in vivo, owing to its unique combination of fast imaging speeds, large field of view, and low phototoxicity. This chapter briefly reviews state-of-the-art technology for variations of light-sheet microscopy. We review recent examples of optogenetics in combination with light-sheet microscopy and discuss some current bottlenecks and horizons of light sheet in all-optical physiology. We describe how 3-dimensional optogenetics can be added to an home-built light-sheet microscope, including technical notes about choices in microscope configuration to consider depending on the time and length scales of interest.
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Weik, Martin H. "microscope." In Computer Science and Communications Dictionary, 1016. Boston, MA: Springer US, 2000. http://dx.doi.org/10.1007/1-4020-0613-6_11513.

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Breinig, Marianne. "Heisenberg Microscope." In Compendium of Quantum Physics, 279–80. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-70626-7_84.

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Mitsuhashi, Jun. "Microscope Photography." In Invertebrate Tissue Culture Methods, 353–55. Tokyo: Springer Japan, 2002. http://dx.doi.org/10.1007/978-4-431-67875-5_37.

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Gross, Herbert, Fritz Blechinger, and Bertram Achtner. "Microscope Optics." In Handbook of Optical Systems, 541–721. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2015. http://dx.doi.org/10.1002/9783527699247.ch7.

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Gooch, Jan W. "Polarizing Microscope." In Encyclopedic Dictionary of Polymers, 547. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_8919.

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Jain, Aakanchha, Richa Jain, and Sourabh Jain. "Compound Microscope." In Basic Techniques in Biochemistry, Microbiology and Molecular Biology, 17–18. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-4939-9861-6_8.

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Wu, Xiaohua. "Fluorescence Microscope." In Encyclopedia of Systems Biology, 744. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-9863-7_1021.

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Elkins, Kelly M. "The microscope." In Introduction to Forensic Chemistry, 33–49. Boca Raton, FL : CRC Press/Taylor & Francis Group, [2019]: CRC Press, 2018. http://dx.doi.org/10.4324/9780429454530-3.

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Weik, Martin H. "simple microscope." In Computer Science and Communications Dictionary, 1591. Boston, MA: Springer US, 2000. http://dx.doi.org/10.1007/1-4020-0613-6_17459.

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Conference papers on the topic "Microscope"

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Webb, Robert H. "Microlaser microscope." In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1990. http://dx.doi.org/10.1364/oam.1990.mpp4.

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A scanned laser microscope with no moving parts is described. The source for this device is an array of 250 000 microlasers on a single substrate, each laser being typically 2 mm in diameter. The entire array is imaged on the microscope's object plane, but only one laser emits light at a given instant. Thus, as the array is electronically scanned, the illumination spot moves over the object. In the simplest configuration, a beam splitter returns light from the object to a detector, and the resulting voltage stream is synchronously displayed on a television monitor. The microscope can thus be packaged in a volume of ~1 cm3 (plus electronics). Variants on the basic microscope permit random access to any pixel, transmission microscopy, and a wide range of field sizes. Later versions will incorporate a synchronous solid-state detector array, which makes this a confocal microscope, and multiplexing of the array elements so that the device has both the scanning laser (confocal) microscope's brightness and the tandem-scanning (confocal) microscope's Felgetts advantage. This detector array can be used to run much faster than television scanning rates or to use much less light, thus avoiding fluorophore bleaching. Ultimately, the microscope can be further miniaturized so that it can be introduced into body cavities and other difficult places. Finally, the same technology can be used to make an inexpensive high-brightness display that is small enough to be worn on an eyeglass frame.
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Masters, Barry R., and Andreas A. Thaer. "Confocal Microscopy of the Human In Vivo Cornea." In Ophthalmic and Visual Optics. Washington, D.C.: Optica Publishing Group, 1993. http://dx.doi.org/10.1364/ovo.1993.osab.2.

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The in-vivo observation of the living cornea by the technique of confocal microscopy provides en face images of high contrast and resolution1-4 . In contrast to Nipkow disk pinhole confocal microscopes,1-4 slit based confocal systems collect more light form the eye.5-6 The development of the wide-field specular microscope by Koester was limited by the low numerical aperture of the applanating cone objective7,8. Recent developments of a high numerical aperture for the wide-field specular microscope has resulted in a confocal microscope for the eye.9,10 We describe a new flying slit confocal microscope, illuminated with a halogen lamp, which has unique imaging characteristics for in vivo human confocal microscopy.
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Incardona, Nicolo, Angel Tolosa, Gabriele Scrofani, Manuel Martinez-Corral, and Genaro Saavedra. "The Lightfield Eyepiece: an Add-on for 3D Microscopy." In 3D Image Acquisition and Display: Technology, Perception and Applications. Washington, D.C.: Optica Publishing Group, 2022. http://dx.doi.org/10.1364/3d.2022.3tu5a.6.

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Fourier lightfield microscopy is an emerging technique for real-time acquisition of three-dimensional microscopic samples. Here, we present the lightfield eyepiece, an add-on device capable of converting any conventional microscope to a Fourier lightfield microscope.
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Conchello, Jose A., J. Peter Zelten, Frank C. Miele, Bruce H. Davis, and Eric W. Hansen. "Enhanced 3-D reconstruction from confocal microscope images." In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1988. http://dx.doi.org/10.1364/oam.1988.thff1.

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Confocal scanning microscopes are known to possess superior optical sectioning capabilities compared to conventional microscopes. Out-of-focus contributions in a through-focus series of images are significantly reduced by the confocal geometry but not completely removed. This paper reports our initial investigations Into a posterioriimage processing (i.e., deconvolution) for further improvement of depth resolution in confocal microscopy. This project is part of a larger effort In laser scanning fluorescence microscopy for biological and biophysical analyses in living cells. The instrument is built around a standard inverted microscope stand, enabling the use of standard optics, micromanipulation apparatus, and conventional (including video) microscopy in conjunction with laser scanning. The beam is scanned across the specimen by a pair of galvanometer-mounted mirrors driven by a programmable controller which can operate In three modes: full raster scan; region of interest; and random-access (point-hopping). After taking a scout image with laser scanning or video, the user will select isolated points or regions of interest for further analysis via a graphic user interface implemented on the system’s host computer. Experimental parameters such as detector integration times are set up with a window-style menu.
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Lima, Rui, Takuji Ishikawa, Motohiro Takeda, Shuji Tanaka, Yo-suke Imai, Ken-ichi Tsubota, Shigeo Wada, and Takami Yamaguchi. "Measurement of Erythrocyte Motions in Microchannels by Using a Confocal Micro-PTV System." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-175969.

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Detailed knowledge on the motion of individual red blood cells (RBCs) flowing in microchannels is essential to provide a better understanding on the blood rheological properties and disorders in microvessels. Several studies on both individual and concentrated RBCs have already been performed in the past [1, 2]. However, all studies used conventional microscopes and also ghost cells to obtain visible trace RBCs through the microchannel. Recently, considerable progress in the development of confocal microscopy and consequent advantages of this microscope over the conventional microscopes have led to a new technique known as confocal micro-PIV [3, 4]. This technique combines the conventional PIV system with a spinning disk confocal microscope (SDCM). Due to its outstanding spatial filtering technique together with the multiple point light illumination system, this kind of microscope has the ability to obtain in-focus images with optical thickness less than 1 μm, a task extremely difficult to be achieved by using a conventional microscope.
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Wegscheider, S., A. Georgi, V. Sandoghdar, G. Krausch, and J. Mlynek. "Scanning near-field optical lithography." In The European Conference on Lasers and Electro-Optics. Washington, D.C.: Optica Publishing Group, 1996. http://dx.doi.org/10.1364/cleo_europe.1996.cfa4.

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The resolution of various scanning probe microscopy methods can be applied to the fabrication of nanostructures. Various methods of local material modification based on different microscopic mechanisms have been proposed, examples of which are : material transfer between a scanning tunneling microscope (STM) tip and a substrate, local oxidation of silicon using atomic force microscope (AFM). Scanning near-field optical microscopy (SNOM) is also an attractive candidate for nanofabrication. Here the optical spot size in the near-field is given by the resolution of the SNOM which in turn is determined by the details of the tip geometry and is typically between 50 and 100 nanometers.
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Chen, Wenbin, Yian Yian, Qiju Zhang, Tongpi Li, Tiechen Guo, Zhong Liu, Huiji Qin, and Daoqin Li. "Computed tomographic microscope: theory of microscopic CT." In Optoelectronic Science and Engineering '94: International Conference, edited by Wang Da-Heng, Anna Consortini, and James B. Breckinridge. SPIE, 1994. http://dx.doi.org/10.1117/12.182059.

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Chmelik, Radim. "Advances in digital holographic microscopy: coherence-controlled microscope." In SPIE Optics + Optoelectronics, edited by Miroslav Hrabovský, Miroslav Miler, and John T. Sheridan. SPIE, 2011. http://dx.doi.org/10.1117/12.888733.

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Zhang, Chaoyun, Marco Fiore, Cezary Ziemlicki, and Paul Patras. "Microscope." In MobiCom '20: The 26th Annual International Conference on Mobile Computing and Networking. New York, NY, USA: ACM, 2020. http://dx.doi.org/10.1145/3372224.3419195.

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Skarlatos, Dimitrios, Mengjia Yan, Bhargava Gopireddy, Read Sprabery, Josep Torrellas, and Christopher W. Fletcher. "MicroScope." In ISCA '19: The 46th Annual International Symposium on Computer Architecture. New York, NY, USA: ACM, 2019. http://dx.doi.org/10.1145/3307650.3322228.

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Reports on the topic "Microscope"

1

Zhang, X. C. Terahertz Microscope. Fort Belvoir, VA: Defense Technical Information Center, May 2010. http://dx.doi.org/10.21236/ada533321.

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Catalyurek, Umit, Michael D. Beynon, Chialin Chang, Tahsin Kurc, Alan Sussman, and Joel Saltz. The Virtual Microscope. Fort Belvoir, VA: Defense Technical Information Center, January 2005. http://dx.doi.org/10.21236/ada440466.

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Crewe, A. V., and O. H. Kapp. Electron microscope studies. Office of Scientific and Technical Information (OSTI), June 1991. http://dx.doi.org/10.2172/6000131.

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Crewe, A. V., and O. H. Kapp. Electron microscope studies. Office of Scientific and Technical Information (OSTI), July 1992. http://dx.doi.org/10.2172/7015892.

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Day, R. D., and P. E. Russell. Atomic Force Microscope. Office of Scientific and Technical Information (OSTI), December 1988. http://dx.doi.org/10.2172/476627.

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Melloch, Michael R. Scanning Probe Microscope. Fort Belvoir, VA: Defense Technical Information Center, March 2001. http://dx.doi.org/10.21236/ada388569.

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George, J. S., D. M. Rector, D. M. Ranken, B. Peterson, and J. Kesteron. Virtual pinhole confocal microscope. Office of Scientific and Technical Information (OSTI), June 1999. http://dx.doi.org/10.2172/353183.

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Taylor, A. J., G. P. Donati, G. Rodriguez, T. R. Gosnell, S. A. Trugman, and D. I. Some. Femtosecond scanning tunneling microscope. Office of Scientific and Technical Information (OSTI), November 1998. http://dx.doi.org/10.2172/672306.

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O'Keefe, Michael A. One-Angstrom microscope update. Office of Scientific and Technical Information (OSTI), April 1999. http://dx.doi.org/10.2172/809890.

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Kenik, E. (Intermediate voltage electron microscope). Office of Scientific and Technical Information (OSTI), November 1989. http://dx.doi.org/10.2172/5356814.

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