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Journal articles on the topic 'Microprinting'

Author: Grafiati

Published: 28 June 2021

Last updated: 15 February 2022

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1

Kowalczyk, Bartlomiej, Mario M. Apodaca, Hideyuki Nakanishi, Stoyan K. Smoukov, and Bartosz A. Grzybowski. "Microprinting: Small 17/2009." Small 5, no. 17 (September 4, 2009): NA. http://dx.doi.org/10.1002/smll.200990086.

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2

Zergioti, I., A. Karaiskou, D. G. Papazoglou, C. Fotakis, M. Kapsetaki, and D. Kafetzopoulos. "Femtosecond laser microprinting of biomaterials." Applied Physics Letters 86, no. 16 (April 18, 2005): 163902. http://dx.doi.org/10.1063/1.1906325.

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3

Lin, Yen-Po, Yong Zhang, and Min-Feng Yu. "Parallel Process 3D Metal Microprinting." Advanced Materials Technologies 4, no. 1 (November 12, 2018): 1800393. http://dx.doi.org/10.1002/admt.201800393.

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4

Doherty, Rachel P., Thijs Varkevisser, Margot Teunisse, Jonas Hoecht, Stefania Ketzetzi, Samia Ouhajji, and Daniela J. Kraft. "Catalytically propelled 3D printed colloidal microswimmers." Soft Matter 16, no. 46 (2020): 10463–69. http://dx.doi.org/10.1039/d0sm01320j.

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5

Zijie Lin, 林子杰, 徐剑 Jian Xu, and 程亚 Ya Cheng. "Laser assisted 3D metal microprinting (Invited)." Infrared and Laser Engineering 49, no. 12 (2020): 20201079. http://dx.doi.org/10.3788/irla.24_invited-1079new.

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6

Zijie Lin, 林子杰, 徐剑 Jian Xu, and 程亚 Ya Cheng. "Laser assisted 3D metal microprinting (Invited)." Infrared and Laser Engineering 49, no. 12 (2020): 20201079. http://dx.doi.org/10.3788/irla20201079.

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7

Mayer, Frederik, Daniel Ryklin, Irene Wacker, Ronald Curticean, Martin Čalkovský, Andreas Niemeyer, Zheqin Dong, et al. "3D Two‐Photon Microprinting of Nanoporous Architectures." Advanced Materials 32, no. 32 (June 30, 2020): 2002044. http://dx.doi.org/10.1002/adma.202002044.

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8

Tavana, Hossein, and Shuichi Takayama. "Aqueous biphasic microprinting approach to tissue engineering." Biomicrofluidics 5, no. 1 (March 2011): 013404. http://dx.doi.org/10.1063/1.3516658.

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9

Serra, P., M. Duocastella, J. M. Fernández-Pradas, and J. L. Morenza. "Liquids microprinting through laser-induced forward transfer." Applied Surface Science 255, no. 10 (March 2009): 5342–45. http://dx.doi.org/10.1016/j.apsusc.2008.07.200.

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10

Verbitsky, Lior, Nir Waiskopf, Shlomo Magdassi, and Uri Banin. "A clear solution: semiconductor nanocrystals as photoinitiators in solvent free polymerization." Nanoscale 11, no. 23 (2019): 11209–16. http://dx.doi.org/10.1039/c9nr03086g.

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Semiconductor nanocrystals are shown as highly efficient quantum photoinitiators for solvent-and-additive-free polymerization with micromolar loading, surpassing traditional organic initiators. The new quantum photoinitiators demonstrate a two-photon polymerization capacity, allowing multi-functional microprinting.

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11

Duan, Hongxu, Cheng Li, Weiwei Yang, Brandon Lojewski, Linan An, and Weiwei Deng. "Near-Field Electrospray Microprinting of Polymer-Derived Ceramics." Journal of Microelectromechanical Systems 22, no. 1 (February 2013): 1–3. http://dx.doi.org/10.1109/jmems.2012.2226932.

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12

Mayer, Frederik, Stefan Richter, Johann Westhauser, Eva Blasco, Christopher Barner-Kowollik, and Martin Wegener. "Multimaterial 3D laser microprinting using an integrated microfluidic system." Science Advances 5, no. 2 (February 2019): eaau9160. http://dx.doi.org/10.1126/sciadv.aau9160.

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Three-dimensional (3D) laser micro- and nanoprinting has become a versatile, reliable, and commercially available technology for the preparation of complex 3D architectures for diverse applications. However, the vast majority of structures published so far have been composed of only a single constituent material. Here, we present a system based on a microfluidic chamber integrated into a state-of-the-art laser lithography apparatus. This system is scalable in terms of the number of materials and eliminates the need to go back and forth between the lithography instrument and the chemistry room numerous times, with tedious realignment steps in between. As an application, we present 3D deterministic microstructured security features requiring seven different liquids: a nonfluorescent photoresist as backbone, two photoresists containing different fluorescent quantum dots, two photoresists with different fluorescent dyes, and two developers. Our integrated microfluidic 3D printing system opens the door to truly multimaterial 3D additive manufacturing on the micro- and nanoscale.

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13

Hirt, Luca, Stephan Ihle, Zhijian Pan, Livie Dorwling-Carter, Alain Reiser, Jeffrey M. Wheeler, Ralph Spolenak, János Vörös, and Tomaso Zambelli. "3D Microprinting: Template-Free 3D Microprinting of Metals Using a Force-Controlled Nanopipette for Layer-by-Layer Electrodeposition (Adv. Mater. 12/2016)." Advanced Materials 28, no. 12 (March 2016): 2277. http://dx.doi.org/10.1002/adma.201670077.

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14

Zergioti, I., S. Mailis, N. A. Vainos, A. Ikiades, C. P. Grigoropoulos, and C. Fotakis. "Microprinting and microetching of diffractive structures using ultrashort laser pulses." Applied Surface Science 138-139 (January 1999): 82–86. http://dx.doi.org/10.1016/s0169-4332(98)00526-1.

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15

Bilal, Muhammad, and Masatoshi Sakairi. "High throughput electrochemically driven metal microprinting with multicapillary droplet cell." Materials Today Communications 26 (March 2021): 102053. http://dx.doi.org/10.1016/j.mtcomm.2021.102053.

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16

Giltinan, Joshua, Varun Sridhar, Ugur Bozuyuk, Devin Sheehan, and Metin Sitti. "3D Microprinting of Iron Platinum Nanoparticle‐Based Magnetic Mobile Microrobots." Advanced Intelligent Systems 3, no. 1 (January 2021): 2170012. http://dx.doi.org/10.1002/aisy.202170012.

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17

Kameya, Yuki, Hiroshi Yamaki, Reiya Ono, and Masahiro Motosuke. "Fabrication of micropillar TiO 2 photocatalyst arrays using nanoparticle-microprinting method." Materials Letters 175 (July 2016): 262–65. http://dx.doi.org/10.1016/j.matlet.2016.04.013.

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18

Braun, Hans-G., and Evelyn Meyer. "Microprinting - a new approach to study competitive structure formation on surfaces." Macromolecular Rapid Communications 20, no. 6 (June 1, 1999): 325–27. http://dx.doi.org/10.1002/(sici)1521-3927(19990601)20:6<325::aid-marc325>3.0.co;2-r.

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19

Petrak, David, Ehsan Atefi, Liya Yin, William Chilian, and Hossein Tavana. "Automated, spatio-temporally controlled cell microprinting with polymeric aqueous biphasic system." Biotechnology and Bioengineering 111, no. 2 (September 11, 2013): 404–12. http://dx.doi.org/10.1002/bit.25100.

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20

McGraw, Gregory J., and Stephen R. Forrest. "Vapor-Phase Microprinting of Multicolor Phosphorescent Organic Light Emitting Device Arrays." Advanced Materials 25, no. 11 (January 20, 2013): 1583–88. http://dx.doi.org/10.1002/adma.201204410.

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21

Patrascioiu, A., M. Duocastella, J. M. Fernández-Pradas, J. L. Morenza, and P. Serra. "Liquids microprinting through a novel film-free femtosecond laser based technique." Applied Surface Science 257, no. 12 (April 2011): 5190–94. http://dx.doi.org/10.1016/j.apsusc.2010.11.093.

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22

Galeotti, Francesco, Ilaria Chiusa, Laura Morello, Silvia Gianì, Diego Breviario, Sonja Hatz, Francesco Damin, Marcella Chiari, and Alberto Bolognesi. "Breath figures-mediated microprinting allows for versatile applications in molecular biology." European Polymer Journal 45, no. 11 (November 2009): 3027–34. http://dx.doi.org/10.1016/j.eurpolymj.2009.08.009.

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23

Slavík, Jan, Josef Skopalík, Ivo Provazník, and Jaromír Hubálek. "Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting." Sensors 19, no. 24 (December 5, 2019): 5379. http://dx.doi.org/10.3390/s19245379.

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Multielectrode arrays (MEAs) are devices for non-invasive electrophysiological measurements of cell populations. This paper describes a novel fabrication method of MEAs with a fully planar surface. The surface of the insulation layer and the surface of the electrodes were on one plane; we named this device the planar MEA (pMEA). The main advantage of the pMEA is that it allows uniform contact between the pMEA surface and a substrate for positioning of microfluidic channels or microprinting of a cell adhesive layer. The fabrication of the pMEA is based on a low adhesive Au sacrificial peel-off layer. In divergence from conventional MEAs with recessed electrodes, the electrodes of the pMEA lead across the sloped edge of the insulation layer. To make this, the profile of the edge of the insulation layer was measured and the impedance of the planar electrodes was characterized. The impedance of the pMEA was comparable with the impedance of conventional MEA electrodes. The pMEA was tested for patterning HL-1 cells with a combination of imprinting fibronectin and coating by antifouling poly (l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG). The HL-1 cells remained patterned even at full confluency and presented spontaneous and synchronous beating activity.

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24

Forrest, Stephen R., and Gregory J. McGraw. "55.1:Invited Paper: Organic Vapor Jet MicroPrinting of OLED Displays and Lighting Panels." SID Symposium Digest of Technical Papers 44, no. 1 (June 2013): 759. http://dx.doi.org/10.1002/j.2168-0159.2013.tb06325.x.

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25

Tsutsumi, Jun’ya. "High-Throughput Nanoparticle Chemisorption Printing of Chemical Sensors with High-Wiring-Density Electrodes." Electronic Materials 2, no. 2 (April 8, 2021): 72–81. http://dx.doi.org/10.3390/electronicmat2020007.

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We report on the high-throughput non-lithographic microprinting of a high-wiring-density interdigitated array electrode (line and space = 5 µm/5 µm), based on a facile wet/dewet patterning of silver nanoparticle ink. The trade-off between high-density wiring and pattern collapse in the wet/dewet patterning is overcome by employing a new herringbone design of interdigitated array electrode. We demonstrate electrochemical sensing of p-benzoquinone by the fabricated interdigitated array electrode, showing a typical steady-state I–V characteristics with superior signal amplification benefiting from the redox cycling effect. Our findings provide a new technical solution for the scalable manufacture of advanced chemical sensors, with an economy of scale that cannot be realized by other techniques.

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26

Li, Chi, Changrui Liao, Jia Wang, Zongsong Gan, and Yiping Wang. "Femtosecond Laser Microprinting of a Polymer Optical Fiber Interferometer for High-Sensitivity Temperature Measurement." Polymers 10, no. 11 (October 26, 2018): 1192. http://dx.doi.org/10.3390/polym10111192.

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Femtosecond laser induced multi-photon polymerization technique can be applied to fabricate an ultracompact polymer optical fiber interferometer which was embedded in a section of hollow core fiber. The production of the photoresin, used in this work, is described. Such a device has been used for temperature measurement, due to its excellent thermal properties. Transmission spectrum, structural morphology, and temperature response of the polymer optical fiber interferometer are experimentally investigated. A high wavelength sensitivity of 6.5 nm/°C is achieved over a temperature range from 25 °C to 30 °C. The proposed polymer optical fiber interferometer exhibits high temperature sensitivity, excellent mechanical strength, and ultra-high integration. More complex fiber-integrated polymer function micro/nano structures produced by this technique may result in more applications in optical fiber communication and optical fiber sensors.

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27

Li, Chi, Changrui Liao, Jia Wang, Zhengyong Li, Ying Wang, Jun He, Zhiyong Bai, and Yiping Wang. "Femtosecond laser microprinting of a polymer fiber Bragg grating for high-sensitivity temperature measurements." Optics Letters 43, no. 14 (July 12, 2018): 3409. http://dx.doi.org/10.1364/ol.43.003409.

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28

Brandolt, Cristiane de Souza, Célia de Fraga Malfatti, Maria Rita Ortega Vega, Gelsa Edith Navarro Hidalgo, and Roberto Moreira Schroeder. "Determination of hydrogen trapping mechanisms by microprinting in Ni and Co coatings obtained by HVOF." Surface and Coatings Technology 362 (March 2019): 262–73. http://dx.doi.org/10.1016/j.surfcoat.2019.01.111.

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29

Li, Zhengyong, Changrui Liao, Jia Wang, Ziliang Li, Peng Zhou, Ying Wang, and Yiping Wang. "Femtosecond Laser Microprinting of a Fiber Whispering Gallery Mode Resonator for Highly-Sensitive Temperature Measurements." Journal of Lightwave Technology 37, no. 4 (February 15, 2019): 1241–45. http://dx.doi.org/10.1109/jlt.2019.2890991.

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30

Zergioti, I., D. G. Papazoglou, A. Karaiskou, N. A. Vainos, and C. Fotakis. "Laser microprinting of InOx active optical structures and time resolved imaging of the transfer process." Applied Surface Science 197-198 (September 2002): 868–72. http://dx.doi.org/10.1016/s0169-4332(02)00440-3.

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31

Zhang, Shihai, Bret Neese, Kailiang Ren, Baojin Chu, Feng Xia, T. Xu, Srinivas Tadigadapa, Qing Wang, Q. M. Zhang, and F. Bauer. "Relaxor Ferroelectric Polymers, Thin Film Devices, and Ink-Jet Microprinting for Thin Film Device Fabrication." Ferroelectrics 342, no. 1 (October 2006): 43–56. http://dx.doi.org/10.1080/00150190600946146.

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32

Hirt, Luca, Stephan Ihle, Zhijian Pan, Livie Dorwling-Carter, Alain Reiser, Jeffrey M. Wheeler, Ralph Spolenak, János Vörös, and Tomaso Zambelli. "Template-Free 3D Microprinting of Metals Using a Force-Controlled Nanopipette for Layer-by-Layer Electrodeposition." Advanced Materials 28, no. 12 (January 19, 2016): 2311–15. http://dx.doi.org/10.1002/adma.201504967.

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33

Cooley, Patrick, David Wallace, and Bogdan Antohe. "Applicatons of Ink-Jet Printing Technology to BioMEMS and Microfluidic Systems." JALA: Journal of the Association for Laboratory Automation 7, no. 5 (October 2002): 33–39. http://dx.doi.org/10.1016/s1535-5535-04-00214-x.

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Applications of microfluidics and MEMS (micro-electromechanical systems) technology are emerging in many areas of biological and life sciences. Non-contact microdispensing systems for accurate, high-throughput deposition of bioactive fluids can be an enabling technology for these applications. In addition to bioactive fluid dispensing, ink-jet based microdispensing allows integration of features (electronic, photonic, sensing, structural, etc.) that are not possible, or very difficult, with traditional photolithographic-based MEMS fabrication methods. Our single fluid and multifluid (MatrixJet™) piezoelectric microdispensers have been used for spot synthesis of peptides, production of microspheres to deliver drugs/biological materials, microprinting of biodegradable polymers for cell proliferation in tissue engineering applications, and spot deposition for DNA, diagnostic immunoassay, antibody and protein arrays. We have created optical elements, sensors, and electrical interconnects by microdeposition of polymers and metal alloys. We have also demonstrated the integration of a reversed phase microcolumn within a piezoelectric dispenser for use in the fractionation of peptides for mass spectrometer analysis.

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34

Moon, Seongjun, Michael S. Jones, Eunbyeol Seo, Jaeyu Lee, Lucas Lahann, Jacob H. Jordahl, Kyung Jin Lee, and Joerg Lahann. "3D jet writing of mechanically actuated tandem scaffolds." Science Advances 7, no. 16 (April 2021): eabf5289. http://dx.doi.org/10.1126/sciadv.abf5289.

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The need for high-precision microprinting processes that are controllable, scalable, and compatible with different materials persists throughout a range of biomedical fields. Electrospinning techniques offer scalability and compatibility with a wide arsenal of polymers, but typically lack precise three-dimensional (3D) control. We found that charge reversal during 3D jet writing can enable the high-throughput production of precisely engineered 3D structures. The trajectory of the jet is governed by a balance of destabilizing charge-charge repulsion and restorative viscoelastic forces. The reversal of the voltage polarity lowers the net surface potential carried by the jet and thus dampens the occurrence of bending instabilities typically observed during conventional electrospinning. In the absence of bending instabilities, precise deposition of polymer fibers becomes attainable. The same principles can be applied to 3D jet writing using an array of needles resulting in complex composite materials that undergo reversible shape transitions due to their unprecedented structural control.

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35

Huang, Nan, Chuen Wai Li, and Barbara Pui Chan. "Multiphoton 3D Microprinting of Protein Micropatterns with Spatially Controlled Heterogeneity - A Platform for Single Cell Matrix Niche Studies." Advanced Biosystems 2, no. 8 (June 10, 2018): 1800053. http://dx.doi.org/10.1002/adbi.201800053.

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36

Watkins, James J., and David J. Bishop. "Fabrication of Sub-45-nm Structures for the Next Generation of Devices: A Lot of Effort for a Little Device." MRS Bulletin 30, no. 12 (December 2005): 937–41. http://dx.doi.org/10.1557/mrs2005.246.

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AbstractFor the last four decades, the feature sizes of electronic devices for computers have been reduced by a factor of two roughly every 18 months. The result has been a tremendous increase in computational power and reduction in the cost of computing, as measured by cost per function, of nearly 30% annually, so that computations can be done for a billionth of the cost of using the technology of the 1950s. However, devices will soon be so small that the current technology used to produce them will have reached its limits, and the graininess of individual atoms will affect their behavior. This issue focuses on techniques to make tiny devices with dimensions under 45 nm (45 billionths of a meter) for the next generation of devices.Techniques start with coupling currently used 193-nm and 157-nm optical lithography with liquid immersion to reduce the effective wavelength. Other techniques include microprinting, self-assembly, templating, and using supercritical fluids to avoid the effects of surface tension while enabling solution-based processing at such small dimensions. The development of three-dimensional structures that are approaching this scale is also discussed.The methods presented will have an effect on many areas of technology, including, in addition to electronics, advanced sensor technology, energy conversion, catalysis, and nanoelectromechanical systems.

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37

Blackiston, Douglas, Emma Lederer, Sam Kriegman, Simon Garnier, Joshua Bongard, and Michael Levin. "A cellular platform for the development of synthetic living machines." Science Robotics 6, no. 52 (March 31, 2021): eabf1571. http://dx.doi.org/10.1126/scirobotics.abf1571.

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Robot swarms have, to date, been constructed from artificial materials. Motile biological constructs have been created from muscle cells grown on precisely shaped scaffolds. However, the exploitation of emergent self-organization and functional plasticity into a self-directed living machine has remained a major challenge. We report here a method for generation of in vitro biological robots from frog (Xenopus laevis) cells. These xenobots exhibit coordinated locomotion via cilia present on their surface. These cilia arise through normal tissue patterning and do not require complicated construction methods or genomic editing, making production amenable to high-throughput projects. The biological robots arise by cellular self-organization and do not require scaffolds or microprinting; the amphibian cells are highly amenable to surgical, genetic, chemical, and optical stimulation during the self-assembly process. We show that the xenobots can navigate aqueous environments in diverse ways, heal after damage, and show emergent group behaviors. We constructed a computational model to predict useful collective behaviors that can be elicited from a xenobot swarm. In addition, we provide proof of principle for a writable molecular memory using a photoconvertible protein that can record exposure to a specific wavelength of light. Together, these results introduce a platform that can be used to study many aspects of self-assembly, swarm behavior, and synthetic bioengineering, as well as provide versatile, soft-body living machines for numerous practical applications in biomedicine and the environment.

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38

Huang, Nan, Chuen Wai Li, and Barbara Pui Chan. "Protein Micropatterns: Multiphoton 3D Microprinting of Protein Micropatterns with Spatially Controlled Heterogeneity - A Platform for Single Cell Matrix Niche Studies (Adv. Biosys. 8/2018)." Advanced Biosystems 2, no. 8 (August 2018): 1870072. http://dx.doi.org/10.1002/adbi.201870072.

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39

"EOS creates 3D microprinting company." Metal Powder Report 69, no. 1 (January 2014): 39. http://dx.doi.org/10.1016/s0026-0657(14)70033-5.

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40

Tomchenko, Alexey, and Brent Marquis. "Nanoparticle Metal-Oxide Films on Microhotplate Platforms: Fabrication and Gas-Sensitive Properties." MRS Proceedings 915 (2006). http://dx.doi.org/10.1557/proc-0915-r07-12.

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AbstractIn this presentation, we discuss the development of nanostructured sensor materials based on nanoparticulate metal-oxide suspensions deposited onto MEMS μHPs by microprinting. The preparation of the suspensions is described; the precise control over the thickness of the films through the composition of the metal-oxide suspensions is demonstrated. The procedure of microprinting is described. The deposited films are evaluated as chemical sensors. The sensor performance of the microsensors – sensitivity, stability, speed of operation, and selectivity – is compared with that of analogous traditional thick-film sensors.

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41

Nishioka, Gary M., Asya L. Borikova, and Charles W. Sokolik. "High Resolution Microprinting With an Electrospray Printer." FASEB Journal 20, no. 4 (March 2006). http://dx.doi.org/10.1096/fasebj.20.4.a527.

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42

Giltinan, Joshua, Varun Sridhar, Ugur Bozuyuk, Devin Sheehan, and Metin Sitti. "3D Microprinting of Iron Platinum Nanoparticle‐Based Magnetic Mobile Microrobots." Advanced Intelligent Systems, November 13, 2020, 2000204. http://dx.doi.org/10.1002/aisy.202000204.

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43

Antonini, Andrea, Andrea Sattin, Monica Moroni, Serena Bovetti, Claudio Moretti, Francesca Succol, Angelo Forli, et al. "Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness." eLife 9 (October 13, 2020). http://dx.doi.org/10.7554/elife.58882.

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Imaging neuronal activity with high and homogeneous spatial resolution across the field-of-view (FOV) and limited invasiveness in deep brain regions is fundamental for the progress of neuroscience, yet is a major technical challenge. We achieved this goal by correcting optical aberrations in gradient index lens-based ultrathin (≤500 µm) microendoscopes using aspheric microlenses generated through 3D-microprinting. Corrected microendoscopes had extended FOV (eFOV) with homogeneous spatial resolution for two-photon fluorescence imaging and required no modification of the optical set-up. Synthetic calcium imaging data showed that, compared to uncorrected endoscopes, eFOV-microendoscopes led to improved signal-to-noise ratio and more precise evaluation of correlated neuronal activity. We experimentally validated these predictions in awake head-fixed mice. Moreover, using eFOV-microendoscopes we demonstrated cell-specific encoding of behavioral state-dependent information in distributed functional subnetworks in a primary somatosensory thalamic nucleus. eFOV-microendoscopes are, therefore, small-cross-section ready-to-use tools for deep two-photon functional imaging with unprecedentedly high and homogeneous spatial resolution.

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44

Frenzel, Tobias, Vincent Hahn, Patrick Ziemke, Jonathan Ludwig Günter Schneider, Yi Chen, Pascal Kiefer, Peter Gumbsch, and Martin Wegener. "Large characteristic lengths in 3D chiral elastic metamaterials." Communications Materials 2, no. 1 (January 4, 2021). http://dx.doi.org/10.1038/s43246-020-00107-w.

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AbstractThree-dimensional (3D) chiral mechanical metamaterials enable behaviors not accessible in ordinary materials. In particular, a coupling between displacements and rotations can occur, which is symmetry-forbidden without chirality. In this work, we solve three open challenges of chiral metamaterials. First, we provide a simple analytical model, which we use to rationalize the design of the chiral characteristic length. Second, using rapid multi-photon multi-focus 3D laser microprinting, we manufacture samples with more than 105 micrometer-sized 3D chiral unit cells. This number surpasses previous work by more than two orders of magnitude. Third, using analytical and numerical modeling, we realize chiral characteristic lengths of the order of ten unit cells, changing the sample-size dependence qualitatively and quantitatively. In the small-sample limit, the twist per axial strain is initially proportional to the sample side length, reaching a maximum at the characteristic length. In the thermodynamic limit, the twist per axial strain is proportional to the square of the characteristic length. We show that chiral micropolar continuum elasticity can reproduce this behavior.

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45

Velasco, Vanessa, S. Ali Shariati, and Rahim Esfandyarpour. "Microtechnology-based methods for organoid models." Microsystems & Nanoengineering 6, no. 1 (October 5, 2020). http://dx.doi.org/10.1038/s41378-020-00185-3.

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Abstract Innovations in biomaterials and stem cell technology have allowed for the emergence of novel three-dimensional (3D) tissue-like structures known as organoids and spheroids. As a result, compared to conventional 2D cell culture and animal models, these complex 3D structures have improved the accuracy and facilitated in vitro investigations of human diseases, human development, and personalized medical treatment. Due to the rapid progress of this field, numerous spheroid and organoid production methodologies have been published. However, many of the current spheroid and organoid production techniques are limited by complexity, throughput, and reproducibility. Microfabricated and microscale platforms (e.g., microfluidics and microprinting) have shown promise to address some of the current limitations in both organoid and spheroid generation. Microfabricated and microfluidic devices have been shown to improve nutrient delivery and exchange and have allowed for the arrayed production of size-controlled culture areas that yield more uniform organoids and spheroids for a higher throughput at a lower cost. In this review, we discuss the most recent production methods, challenges currently faced in organoid and spheroid production, and microfabricated and microfluidic applications for improving spheroid and organoid generation. Specifically, we focus on how microfabrication methods and devices such as lithography, microcontact printing, and microfluidic delivery systems can advance organoid and spheroid applications in medicine.

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Kz, Kshitiz, David E. Ellison, Junaid Afzal, Maimon E. Hubbi, Segun Bernard, Ruchi Goyal, Connie Chang, Roselle Abraham, and Andre C. Levchenko. "Abstract 19641: Novel Microprinted Elisa Platform for High Throughput Screening of Protein Secretion Reveals a Comprehensive Strategy to Prevent Ischemia Reperfusion Induced Apoptosis." Circulation 130, suppl_2 (November 25, 2014). http://dx.doi.org/10.1161/circ.130.suppl_2.19641.

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Introduction: A major challenge in using stem cells to treat myocardial infarction (MI) is the massive cell death post transplant. Many progenitor cell types limit disrepair post MI without significant cardiac differentiation, possibly by anti-apoptotic signals in their secretions. The signature of these secretions is however not well known. Methods: We created a novel high throughput microELISA platform by combining microprinting and microfluidics to precisely estimate secretions of a small population of cells over time. Screening the secretions in many stem cell types used previously to treat MI, i.e. BMSCs (Bone marrow derived stem cells), CDCs (Cardiosphere derived cells), and iPSC-CMs (induced pluripotent stem cell derived cardiomyocytes), we found a common preserved secretory signature of growth factors. Results: Using a high throughput screen of pro-apoptotic factors that prevent CDCs from peroxide induced cell death, we surprisingly found that these factors were identical to the preserved secretory signature. Using the constituents of the anti-apoptotic secretory signature in combination with ischemic and mechanical preconditioning in myocardium mimicking rigidity, we created a comprehensive cytoprotective cocktail to prevent CDCs from ischemia induced cell death. We tested the cocktail in a rat model of ischemia reperfusion, and found a stark reduction in CDC retention post injection. Figures: (A) MicroELISA schematic. Secretions from cells in left are detected by rows of microprinted capture antibodies; photograph in (C-D). Bioluminescence imaging of CDCs treated with comprehensive cocktail show significantly high cell retention vs untreated CDCs.

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Zou, Mengqiang, Changrui Liao, Shen Liu, Cong Xiong, Cong Zhao, Jinlai Zhao, Zongsong Gan, et al. "Fiber-tip polymer clamped-beam probe for high-sensitivity nanoforce measurements." Light: Science & Applications 10, no. 1 (August 27, 2021). http://dx.doi.org/10.1038/s41377-021-00611-9.

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AbstractMicromanipulation and biological, material science, and medical applications often require to control or measure the forces asserted on small objects. Here, we demonstrate for the first time the microprinting of a novel fiber-tip-polymer clamped-beam probe micro-force sensor for the examination of biological samples. The proposed sensor consists of two bases, a clamped beam, and a force-sensing probe, which were developed using a femtosecond-laser-induced two-photon polymerization (TPP) technique. Based on the finite element method (FEM), the static performance of the structure was simulated to provide the basis for the structural design. A miniature all-fiber micro-force sensor of this type exhibited an ultrahigh force sensitivity of 1.51 nm μN−1, a detection limit of 54.9 nN, and an unambiguous sensor measurement range of ~2.9 mN. The Young’s modulus of polydimethylsiloxane, a butterfly feeler, and human hair were successfully measured with the proposed sensor. To the best of our knowledge, this fiber sensor has the smallest force-detection limit in direct contact mode reported to date, comparable to that of an atomic force microscope (AFM). This approach opens new avenues towards the realization of small-footprint AFMs that could be easily adapted for use in outside specialized laboratories. As such, we believe that this device will be beneficial for high-precision biomedical and material science examination, and the proposed fabrication method provides a new route for the next generation of research on complex fiber-integrated polymer devices.

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