Relevant bibliographies by topics / Microinjection

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Academic literature on the topic 'Microinjection'

Author: Grafiati
Published: 4 June 2021
Last updated: 20 February 2023

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Journal articles on the topic "Microinjection"

1

Riley, Jonathan P., Bethwel Raore, Jason S. Taub, Thais Federici, and Nicholas M. Boulis. "Platform and Cannula Design Improvements for Spinal Cord Therapeutics Delivery." Operative Neurosurgery 69, suppl_2 (April 5, 2011): ons147—ons155. http://dx.doi.org/10.1227/neu.0b013e3182195680.

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Abstract BACKGROUND Only recently have data been published attempting to validate a technology and technique suitable for targeted delivery of biological payloads to the human spinal cord. OBJECTIVE To characterize the development and evolution of a spine-stabilized microinjection platform as a vehicle for biologics delivery to the cervical and thoracolumbar spine on the basis of preclinical experience in both non–Good Laboratory Practice (GLP) experimental series and GLP studies. METHODS Our laboratory completed > 100 cervical and lumbar porcine microinjection procedures between July 2004 and June 2010. This included both non–GLP- and GLP-adherent survival series to validate the safety and accuracy achievable with intraspinal microinjection. During this time, 3 different microinjection platforms, injection stages, and cannula designs were tested. RESULTS Repetitive technological improvements reduced incision length, decreased procedural complexity, and simplified ventral horn targeting and accuracy. These changes reduced procedural invasiveness and the likelihood of neurological morbidity while improving targeting accuracy. In part as a result of these technological improvements and procedural modifications, we have safely progressed from single unilateral microinjections to multiple bilateral injections without long-term neurological sequelae. CONCLUSION Technological and procedural refinements have significantly enhanced the capabilities of intraspinal microinjection-based biologics delivery. Reductions in procedural invasiveness and the capability to deliver sequential biological payloads effectively have broadened the flexibility of intraspinal microinjection to a widened array of intrinsic spinal cord pathologies. These advances have laid the groundwork for clinical translation of spinal cord microinjections.
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Willette, R. N., and C. F. Sauermelch. "Abluminal effects of endothelin in cerebral microvasculature assessed by laser-Doppler flowmetry." American Journal of Physiology-Heart and Circulatory Physiology 259, no. 6 (December 1, 1990): H1688—H1693. http://dx.doi.org/10.1152/ajpheart.1990.259.6.h1688.

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Laser-Doppler flowmetry was used to assess the intraparenchymal effects of endothelin 1 (ET-1) on cortical microvascular perfusion (CP). The initial part of this study examined effects of the intraparenchymal microinjection technique on local cortical microvascular responsivity. In anesthetized rats, the microinjection of vehicle (saline) beneath the cortical surface did not alter CO2 responsivity or autoregulation of the cortical microvasculature. In addition, predictable monophasic changes in local CP were elicited by the intraparenchymal microinjection of known vasodilators and vasoconstrictors, i.e., nitroprusside and prostaglandin F2 alpha, respectively. These experiments demonstrate normal responsivity of the cortical microvasculature after intraparenchymal microinjection. In the second part of this study, intraparenchymal microinjections (100 nl) of ET-1 (1-1,000 fmol) were evaluated. Microinjections of less than 10 fmol of ET-1 did not alter CP. However, doses between 10 and 1,000 fmol of ET-1 elicited monophasic dose-related reductions in CP. At 1,000 fmol, the highest dose studied, ET-1 produced a complex microvascular response consisting of an initial profound reduction in CP followed by alternating cycles of increased perfusion ultimately lapsing back to a prolonged period of hypoperfusion. No significant changes in blood pressure were observed after ET-1 administration, and no significant changes in any of the hemodynamic variables were observed after vehicle microinjection. These results suggest that abluminal microvascular actions of ET-1 mediate cerebral vasoconstriction and hypoperfusion.
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Pokrovskiy, V. M., E. A. Patrakhanov, A. Yu Karagodina, Yu V. Stepenko, N. E. Kazban, A. V. Turpakova, O. B. Altukhova, P. R. Lebedev, and A. V. Deykin. "METHODOLOGICAL RECOMMENDATIONS FOR THE USE OF REPRODUCTIVE TECHNOLOGIES TO CREATE GENETICALLY MODIFIED RABBITS." Bulletin of Udmurt University. Series Biology. Earth Sciences 32, no. 4 (December 27, 2022): 439–48. http://dx.doi.org/10.35634/2412-9518-2022-32-4-439-448.

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In this article, we present some practical recommendations that we developed in the course of two years of work on obtaining several lines of rabbits with an artificially modified genome. The technology of obtaining genetically modified rabbits, based on obtaining dated fertilized eggs with further microinjection of genetic constructs, assessment of survival and embryo transfer by laparoscopic and laparotomic methods. The following regimen demonstrated the greatest efficiency: administration of serum gonadotropin (Follimag, MOSAGROGEN, Russia) at a dose of 4ME/kg subcutaneously and intravenous administration of human chorionic gonadotropin (Chorulon, Merck Animal Health, USA) at a dose of 60ME/kg. Thus, the resulting operating procedure allows the operator to perform microinjections with high transfer efficiency and maximum cell survival. Microinjection is carried out through an Eppendorf FemtoJet 4i pneumatic microinjector using hydraulic micromanipulators under visual control through a NIKON ECLIPSE TS2R inverted research microscope. The described results may be useful in the compilation of standard operating procedures used in laboratories that are engaged in the production of genetically modified rabbit animals.
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Riley, Jonathan, Jonathan Glass, Eva L. Feldman, Meraida Polak, Jane Bordeau, Thais Federici, Karl Johe, and Nicholas M. Boulis. "Intraspinal Stem Cell Transplantation in Amyotrophic Lateral Sclerosis." Neurosurgery 74, no. 1 (September 5, 2013): 77–87. http://dx.doi.org/10.1227/neu.0000000000000156.

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Abstract BACKGROUND: The first US Food and Drug Administration approved clinical trial for a stem cell-based treatment of amyotrophic lateral sclerosis has now been completed. OBJECTIVE: Primary aims assessed the safety of a direct microinjection-based technique and the toxicity of neural stem cell transplantation to the ventral horn of the cervical and thoracolumbar spinal cord. Results from thoracolumbar-only microinjection groups have been previously published. Cervical and cervical plus thoracolumbar microinjection group perioperative morbidity results are presented. METHODS: Eighteen microinjection procedures (n = 12 thoracolumbar [T10/11], n = 6 cervical [C3-5]) delivered NSI-566RSC (Neuralstem, Inc), a human neural stem cell, to 15 patients in 5 cohorts. Each injection series comprised 5 injections of 10 μL at 4-mm intervals. The patients in group A (n = 6) were nonambulatory and received unilateral (n = 3) or bilateral (n = 3) thoracolumbar microinjections. The patients in groups B to E were ambulatory and received either unilateral (group B, n = 3) or bilateral (group C, n = 3) thoracolumbar microinjection. Group D and E patients received unilateral cervical (group D, n = 3) or cervical plus bilateral thoracolumbar microinjection (group E, n = 3). RESULTS: Unilateral cervical (group D, n = 3) and cervical plus thoracolumbar (group E, n = 3) microinjections to the ventral horn have been completed in ambulatory patients. One patient developed a postoperative kyphotic deformity prompting completion of a laminoplasty in subsequent patients. Another required reoperation for wound dehiscence and infection. The solitary patient with bulbar amyotrophic lateral sclerosis required perioperative reintubation. CONCLUSION: Delivery of a cellular payload to the cervical or thoracolumbar spinal cord was well tolerated by the spinal cord in this vulnerable population. This encouraging finding supports consideration of this delivery approach for neurodegenerative, oncologic, and traumatic spinal cord afflictions.
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Dean, David A., and Joshua Z. Gasiorowski. "Microinjecting Cells Using a Pulsed-Flow Microinjection System." Cold Spring Harbor Protocols 2011, no. 3 (March 2011): prot5589. http://dx.doi.org/10.1101/pdb.prot5589.

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Dean, David A., and Joshua Z. Gasiorowski. "Microinjecting Cells Using a Constant-Flow Microinjection System." Cold Spring Harbor Protocols 2011, no. 3 (March 2011): prot5590. http://dx.doi.org/10.1101/pdb.prot5590.

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Colombari, Eduardo, Robin L. Davisson, Richard A. Shaffer, William T. Talman, and Stephen J. Lewis. "Hemodynamic effects ofl-glutamate in NTS of conscious rats: a possible role of vascular nitrosyl factors." American Journal of Physiology-Heart and Circulatory Physiology 274, no. 4 (April 1, 1998): H1066—H1074. http://dx.doi.org/10.1152/ajpheart.1998.274.4.h1066.

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This study examined peripheral mechanisms responsible for changes in mean arterial blood pressure, heart rate, and renal, mesenteric, and hindquarter vascular resistances produced by microinjections of l-glutamate (l-Glu) into the nucleus tractus solitarii (NTS) of conscious rats. Microinjection ofl-Glu produced an initial pressor response, bradycardia, and vasoconstriction in each vascular bed. Subsequent hindquarter vasodilation was observed. After prazosin was administered, l-Glu produced initial hypotension that was probably due to reduced cardiac output. This hypotension was followed by hindquarter vasodilation. Inhibition of nitric oxide synthesis did not affect the initial hypotension or bradycardia in rats treated with prazosin, but the first microinjection of l-Glu after administration of prazosin and N G-nitro-l-arginine methyl ester (l-NAME) produced significantly greater hindquarter vasodilation than after administration of prazosin alone. Second and third microinjections ofl-Glu produced significantly smaller hindquarter vasodilation. We conclude that 1) hemodynamic effects produced by microinjection of l-Glu into the NTS of conscious rats involves activation of the sympathetic nervous system and 2) release of preformed nitrosyl factors may mediate vasodilation in the hindquarter vascular bed.
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Avanzino, G. L., P. Ruggeri, D. Blanchi, C. E. Cogo, R. Ermirio, and L. C. Weaver. "GABAB receptor-mediated mechanisms in the RVLM studied by microinjections of two GABAB receptor antagonists." American Journal of Physiology-Heart and Circulatory Physiology 266, no. 5 (May 1, 1994): H1722—H1728. http://dx.doi.org/10.1152/ajpheart.1994.266.5.h1722.

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The cardiovascular effects of microinjections of two gamma-aminobutyric acid (GABA) antagonists specifically acting on GABAB receptors, 2-hydroxy-saclofen (2-OH-S) and CGP-35348, into vasopressor sites of rostral ventrolateral medulla (RVLM) were studied in rats anesthetized with urethan. Bilateral microinjection of 2-OH-S induced significant increases in mean arterial pressure (MAP) and heart rate (HR) in 21 of 26 RVLM vasopressor sites (81%); average increases obtained in the 26 sites studied were +25.2 +/- 3.0 mmHg and +12.7 +/- 2.1 beats/min. Bilateral microinjection of CGP-35348 induced significant increases in MAP and HR in 10 of 12 RVLM sites (83%). Average increases in the 12 sites studied were +27.6 +/- 3.9 mmHg and +14.6 +/- 2.5 beats/min. Sixteen rats received unilateral electrolytic lesions of one RVLM. Microinjections of either 2-OH-S or CGP-35348 into vasopressor sites within the intact RVLM significantly antagonized the depressor responses observed after injections of baclofen (20 pmol) into the same sites, whereas both GABAB antagonists did not affect the depressor response induced by microinjection of muscimol (5 pmol). These results suggest a tonic inhibitory mechanism within the RVLM mediated by GABAB receptors involved in central cardiovascular regulation.
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Nason, Malcolm W., and Peggy Mason. "Modulation of Sympathetic and Somatomotor Function by the Ventromedial Medulla." Journal of Neurophysiology 92, no. 1 (July 2004): 510–22. http://dx.doi.org/10.1152/jn.00089.2004.

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The ventromedial medulla is implicated in a variety of functions including nociceptive and cardiovascular modulation and the control of thermoregulation. To determine whether single microinjections into the ventromedial medulla elicit changes in one or multiple functional systems, the GABAA receptor antagonist bicuculline was microinjected (70 nl, 5–50 ng) into the ventromedial medulla of lightly anesthetized rats, and cardiovascular, respiratory, and nociceptive measures were recorded. Bicuculline microinjection into either the midline raphe or the laterally adjacent reticular nucleus simultaneously increased interscapular brown adipose tissue temperature, heart rate, blood pressure, expired [CO2], and respiration rate and elicited shivering. Bicuculline microinjection also decreased the noxious stimulus-evoked changes in heart rate and blood pressure, decreased the frequency of heat-evoked sighs, and suppressed the cortical desynchronization evoked by noxious stimulation. Although bicuculline suppressed the motor withdrawal evoked by noxious tail heat, it enhanced the motor withdrawal evoked by noxious paw heat, evidence for specifically patterned nociceptive modulation. Saline microinjections into midline or lateral sites had no effect on any measured variable. All bicuculline microinjections, midline or lateral, evoked the same set of physiological effects, consistent with the lack of a topographical organization within the ventromedial medulla. Furthermore, as predicted by the isodendritic morphology of cells in the ventromedial medulla, midline bicuculline microinjection increased the number of c-fos immunoreactive cells in both midline raphe and lateral reticular nuclei. In summary, 70-nl microinjections into ventromedial medulla activate cells in multiple nuclei and elicit increases in sympathetic and somatomotor tone and a novel pattern of nociceptive modulation.
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Carstens, E. "Responses of Rat Spinal Dorsal Horn Neurons to Intracutaneous Microinjection of Histamine, Capsaicin, and Other Irritants." Journal of Neurophysiology 77, no. 5 (May 1, 1997): 2499–514. http://dx.doi.org/10.1152/jn.1997.77.5.2499.

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Carstens, E. Responses of rat spinal dorsal horn neurons to intracutaneous microinjection of histamine, capsaicin, and other irritants. J. Neurophysiol. 77: 2499–2514, 1997. To investigate the spinal processing of cutaneous pruritic and algesic stimuli, single-unit recordings were made from wide-dynamic-range-type lumbar spinal dorsal horn neurons in pentobarbital-sodium-anesthetized rats. Neuronal responses were recorded to mechanical and noxious thermal stimuli, as well as to microinjection (1 μl) of histamine (0.01–10% = 9 × 10−1–9 × 10−4 M), capsaicin (0.1% = 3.3 × 10−3 M), or other algesic chemicals into skin within the receptive field via intracutaneously placed needles. Most (84%) of the 89 neurons responded to intracutaneous (ic) microinjection of histamine with a brief phasic discharge followed by an afterdischarge of variable (s to min) duration. Ten minutes after ic microinjection of histamine (but not NaCl), there was a significant increase in the mean area of the low-threshold (but not high-threshold) portion of unit mechanical receptive fields. However, responses to graded pressure stimuli were not significantly affected after histamine. Responses did not exhibit significant tachyphylaxis when histamine microinjections were repeated at 5- or 10-min intervals. Unit responses significantly increased in a dose-related manner to microinjection of histamine at concentrations ranging across 4 orders of magnitude. Within 30 s after ic microinjection of the H1 antagonist cetirizine, unit responses to ic histamine delivered at the same skin site were significantly attenuated. Unit responses to histamine, as well as to noxious thermal stimulation, were significantly reduced after systemic administration of morphine (3.5 mg/kg ip) in a naloxone-reversible manner. Application of a mechanical rub, scratch, or a noxious heat stimulus during the unit's ongoing response to ic histamine produced a brief and marked excitation, often followed by a period of reduced ongoing discharge. Unit responses to histamine were markedly suppressed by electrical stimulation in the midbrain periaqueductal gray. Most (79%) histamine-responsive units tested also responded to ic microinjection of capsaicin. After the initial microinjection of capsaicin, subsequent responses to histamine and capsaicin microinjections were significantly reduced. Units also responded to ic ethanol (capsaicin vehicle) in a dose-related manner, and showed tachyphylaxis to repeated ic ethanol at 80% but not at 8%. The mean response to 80% ethanol was significantly smaller than to 0.1% capsaicin. All units tested also responded to topical application of mustard oil (50%) and ic serotonin (30 μg). The results are discussed in terms of theories that attempt to reconcile psychophysical and clinical observations of pain and itch sensation.
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Dissertations / Theses on the topic "Microinjection"

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Lawrence, W. A. "Microinjection of tobacco protoplasts." Thesis, University of East Anglia, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372559.

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Martanto, Wijaya. "Microinjection Into Skin Using Microneedles." Diss., Georgia Institute of Technology, 2005. http://hdl.handle.net/1853/11645.

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The development of microneedles that penetrate the skin barrier, but are small enough not to stimulate nerves, has the potential to deliver drugs across skin in a painless way. Controlled injection by convective flow into skin using hollow microneedles, however, has remained a challenge. To address this challenge, the goals of this study were (i) to provide experimental measurements coupled with numerical simulations to quantitatively describe fluid mechanics of flow within microneedles over a range of experimental conditions and needle geometries, (ii) to demonstrate and study the effects of diffusion-based delivery of insulin to diabetic rats in vivo using solid and hollow microneedles and (iii) to determine the effect of experimental parameters on microinfusion through hollow microneedles into skin to optimize drug delivery protocols and identify rate-limiting barriers to flow. Experimentally, we quantified the relationship between pressure drop and flow rate through microneedles as a function of fluid viscosity and microneedle length, diameter, and cone half-angle. Microneedle tip diameter and taper angle were the primary controlling parameters for flow through conically tapered microneedles as shown by numerical simulations. Flow rates over a range of 1.4 56 l/s were achieved through microneedles (in the absence of skin) with pressure drops in the range of 4.6 196.5 kPa. This work also studied the use of solid and hollow microneedle arrays to insert into the skin of diabetic animals for transdermal delivery of insulin. Blood glucose levels dropped by as much as 80% in diabetic rats in vivo. Larger drops in blood glucose level and larger plasma insulin concentrations were shown due to higher donor solution insulin concentration, shorter microneedles insertion time and fewer repeated insertions. The final scope of this work was to determine the effect of microneedle geometry and infusion protocols on microinfusion flow rate into skin in vitro. Infusion flow rates ranged from 21 to 1130 l/h was demonstrated using glass microneedles. The presence of a bevel at the microneedle tip, larger retraction distance and insertion depth, larger infusion pressure and the presence of hyaluronidase led to larger infusion flow rates.
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Bou, malhab Nada. "Moulage par microinjection des polymères semi-cristallins." Phd thesis, Paris, ENSAM, 2012. http://pastel.archives-ouvertes.fr/pastel-00831028.

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La miniaturisation des pièces est une étape importante pour la progression de la microtechnologie dans plusieurs domaines (connectique, médical, optique, microsystèmes mécaniques). Pour cela, le moulage par microinjection, semble être la solution clé pour la production à grande échelle de micro-composants de polymères. Pour les polymères semi-cristallins, la cristallisation, sous fort taux de cisaillement et sous des vitesses de refroidissement élevées (about 100 K/s), induit des morphologies et des propriétés spécifiques. Elle prend donc une importance considérable dans le processus de microinjection par rapport au moulage par injection classique où les épaisseurs injectées sont généralement supérieures à 1 mm. Ces microstructures ont une grande influence sur les propriétés mécaniques du produit final. La prédiction de ces propriétés à partir de la description de la microstructure est un défi technique et scientifique. Durant cette thèse, deux polymères semi-cristallins ont été microinjectés, le polyéthylène haute densité et le polyamide 12. Les analyses obtenues par la microscopie otiques montrent que les morphologies cristallines varient entre les micro- et les macro-pièces. Tandis que la morphologie de 'peau-cœur' est présente dans les macropièces, les micropièces présentent une morphologie plutôt particulière. Les analyses combinées de diffusion et de diffraction des rayons X (SAXS et WAXS) avec un microfaisceau synchrotron, nous ont permis de déterminer la microstructure induite par le processus de microinjection dans toute l'épaisseur des pièces. Nous avons constaté que la morphologie et les orientations cristallines induites sont très dépendantes des conditions d'injection ou de microinjection. Une diminution de l'épaisseur, de la vitesse et de la température du moule, augmente l'orientation cristalline en limitant la relaxation des chaînes de polymères.
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Ward, Kenneth Glenn. "Microinjection and regeneration of tobacco and potato protoplasts." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303971.

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Silhol, Michelle. "La microinjection dans les cellules somatiques : effet d'agents antiviraux." Montpellier 2, 1987. http://www.theses.fr/1987MON20232.

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Silhol, Michelle. "La Microinjection dans les cellules somatiques effet d'agents antiviraux /." Grenoble 2 : ANRT, 1987. http://catalogue.bnf.fr/ark:/12148/cb37609920n.

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Wang, Guang Wei. "Position and force control for piezo-driven microinjection system." Thesis, University of Macau, 2018. http://umaclib3.umac.mo/record=b3951592.

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Kinoshita, Masato, and Kenjiro Ozato. "Cytoplasmic microinjection of DNA into fertilized medaka (Oryzias latipes) eggs." Laboratory of Freshwater Fish Stocks Bioscience Center Nagoya University, 1995. http://hdl.handle.net/2237/13806.

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McKenna, John E. (John Erwin). "Analgesic effects of lidocaine microinjection into the rat dentate gyrus." Thesis, McGill University, 1990. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=59653.

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Previous studies in our laboratory have indicated that anesthetic block of neural activity at discrete sites within the limbic system, including the lateral hypothalamus and anterior cingulum bundle, causes a significant long-lasting analgesia during the formalin test. In this experiment, the local anesthetic lidocaine was microinjected into the dentate region of the hippocampus, an important limbic structure presumed to subserve the affective-motivational aspects of pain. The dentate gyrus is strategically situated at a point of convergence of widespread polysensory cortical input to the hippocampus, to allow modulation of cortical signals before they diverge into numerous limbic circuits. The results indicate that anesthetic block of the anterior region of the dentate gyrus produces analgesia in the rat during the formalin test. The analgesia produced by this procedure became apparent 30 minutes after regional block contralateral to the site of injury and persisted for the duration of the test period. These data provide further evidence that limbic forebrain structures are involved in pain and analgesia.
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Ladjal, Hamid. "Développement d'un simulateur haptique pour la cacaractérisation et la microinjection cellulaires." Thesis, Orléans, 2010. http://www.theses.fr/2010ORLE2019/document.

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L'objectif fondamental de cette thèse est de développer et de mettre en oeuvre un outil interactif desimulation des techniques de micromanipulation biologiques de cellules. Au moyen de cet outil, l'opérateurpourra se former, s'entraîner et améliorer sa maîtrise en développant une gestuelle proche de celle exécutéeen réalité. La conception d'un tel environnement de simulation en temps-réel nécessite de trouver uncompromis entre le réalisme des modèles de comportement biomécanique utilisés, la précision et la stabilitédes algorithmes des méthodes de résolution et de rendu haptique utilisées ainsi que la vitesse de calcul. Lamodélisation mécanique retenue repose sur l'utilisation du modèle hyperélastique de St Venant-Kirchhoff etune formulation dynamique explicite éléments-finis du type masses-tenseurs. Le bien-fondé de cettemodélisation est vérifié sur des essais de microindentation par Microscopie à Force Atomique (AFM) decellules souches embryonnaires de souris et de microinjection d'ovocytes. Nous avons développé etimplémenté des modèles d'interaction en temps-réel qui s'articulent autour de la détection et la gestionrapide des collisions entre outil/cellule.La synthèse du rendu haptique fourni à l'opérateur est également proposée par l'intermédiaire d'un couplagevirtuel. Pour chaque application, nous avons justifié nos choix méthodologiques et Algorithmiques qui sontguidés par les contraintes de "réalisme+précision" "temps-réel". Les différents modèles proposés ont étéintégrés dans le simulateur SIMIC que nous avons développé pendant cette thèse. Ce dernier est dédié à lasimulation interactive pour l'aide à l'apprentissage du geste de microinjection et de nanoindentationcellulaire
The fundamental objective of this thesis is to develop and implementing an interactive simulation techniquesfor micromanipulation biological cells. Using this tool, the operator can form, train and improve its control bydeveloping a gesture similar to that performed in reality. The design of such a simulation environment in realtime requires a compromise between the realism of biomechanical models used the accuracy and stability ofalgorithms and solution methods used haptic rendering and computational speed. Modeling Mechanicalrestraint involves the use of hyperelastic model of St Venant-Kirchhoff formulation and explicit dynamic finiteelement-type mass tensors. The validity of this model is tested on microindentation tests by Atomic ForceMicroscopy (AFM) of mouse embryonic stem cells and microinjection of oocytes. We have developed andimplemented models of real-time interaction that revolve around the detection and management of rapidcollisions between tool / cell.The synthesis of the haptic feedback provided to the operator is also available through a virtual coupling. Foreach application, we have justified our methodological choices and Algorithms that are guided by theconstraints of realism + precision "" real time ". The various proposed models have been integrated into thesimulator SIMIC that we developed during this thesis. This is dedicated to interactive simulation to supportlearning of gesture microinjection and cell nanoindentation
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Books on the topic "Microinjection"

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Microinjection: Methods and applications. New York. N.Y: Humana Press, 2009.

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Carlos, Lacal Juan, Perona Rosario 1956-, and Feramisco James, eds. Microinjection. Basel: Birkhäuser Verlag, 1999.

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Liu, Chengyu, and Yubin Du, eds. Microinjection. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8831-0.

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Carroll, David J., ed. Microinjection. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-202-1.

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Lacal, Juan Carlos, James Feramisco, and Rosario Perona, eds. Microinjection. Basel: Birkhäuser Basel, 1999. http://dx.doi.org/10.1007/978-3-0348-8705-2.

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Cid-Arregui, Angel, and Alejandro García-Carrancá, eds. Microinjection and Transgenesis. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-80343-7.

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Lawrence, Wendy Ann. Microinjection of tobacco protoplasts. Norwich: University of East Anglia, 1986.

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1959-, Cid-Arregui Angel, and García-Carrancá Alejandro 1955-, eds. Microinjection and transgenesis: Strategies and protocols. Berlin: Springer, 1998.

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Seidman, Shlomo. Transgenic Xenopus: Microinjection methods and developmental neurobiology. Totowa, N.J: Humana Press, 1997.

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Bach, Peter H., C. Hugh Reynolds, Jessica M. Clark, John Mottley, and Phil L. Poole, eds. Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4615-2828-9.

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Book chapters on the topic "Microinjection"

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Feramisco, J., R. Perona, and J. C. Lacal. "Needle Microinjection: A Brief History." In Microinjection, 9–15. Basel: Birkhäuser Basel, 1999. http://dx.doi.org/10.1007/978-3-0348-8705-2_1.

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Mathias, R. S., and H. E. Ives. "Role of the InsP3 Receptor in Intracellular Ca2+ Release and Ca2+ Entry." In Microinjection, 123–33. Basel: Birkhäuser Basel, 1999. http://dx.doi.org/10.1007/978-3-0348-8705-2_10.

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Lacal, J. C. "Microinjection of Xenopus laevis Oocytes: A Model System." In Microinjection, 134–45. Basel: Birkhäuser Basel, 1999. http://dx.doi.org/10.1007/978-3-0348-8705-2_11.

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Carnero, A., and J. C. Lacal. "Xenopus laevis Oocytes as a Model for Studying the Activation of Intracellular Kinases." In Microinjection, 146–60. Basel: Birkhäuser Basel, 1999. http://dx.doi.org/10.1007/978-3-0348-8705-2_12.

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Carnero, A., and J. C. Lacal. "Signal Transduction in Xenopus laevis Oocytes by Ras Oncoproteins and Lipid Metabolites." In Microinjection, 161–73. Basel: Birkhäuser Basel, 1999. http://dx.doi.org/10.1007/978-3-0348-8705-2_13.

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Murakami, M. S., and G. F. Vande Woude. "The Multiple Roles of Mos during Meiosis." In Microinjection, 174–86. Basel: Birkhäuser Basel, 1999. http://dx.doi.org/10.1007/978-3-0348-8705-2_14.

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Allison, L. A. "Use of the Xenopus Oocyte System to Study RNA Transport." In Microinjection, 187–98. Basel: Birkhäuser Basel, 1999. http://dx.doi.org/10.1007/978-3-0348-8705-2_15.

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de la Peña, P., and F. Barros. "Functional Expression of G Protein-Coupled Receptors in Xenopus laevis Oocytes." In Microinjection, 199–210. Basel: Birkhäuser Basel, 1999. http://dx.doi.org/10.1007/978-3-0348-8705-2_16.

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Seatter, M. J., and G. W. Gould. "Expression of Glucose Transporters in Xenopus laevis Oocytes: Applications for the Study of Membrane Transporter Function and Regulation." In Microinjection, 211–22. Basel: Birkhäuser Basel, 1999. http://dx.doi.org/10.1007/978-3-0348-8705-2_17.

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Kühl, M., M. Walter, J. Clement, H. Friedle, D. Wedlich, and W. Knöchel. "DNA Injection into Xenopus laevis Embryos as a Tool to Study Spatial Gene Activity." In Microinjection, 223–32. Basel: Birkhäuser Basel, 1999. http://dx.doi.org/10.1007/978-3-0348-8705-2_18.

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Conference papers on the topic "Microinjection"

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Aten, Quentin T., Brian D. Jensen, and Sandra H. Burnett. "Testing of a Pumpless MEMS Microinjection Needle Employing Electrostatic Attraction and Repulsion of DNA." In ASME 2008 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2008. http://dx.doi.org/10.1115/detc2008-49548.

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The ultimate goal of this work is to develop an automated MEMS-based lab-on-a-chip microinjector. This paper outlines one phase of that work: testing the feasibility of a pumpless, polysilicon MEMS microneedle for use in the proposed MEMS-based lab-on-a-chip microinjector. The pumpless MEMS microneedle operates on the principle of attraction and repulsion of DNA using electrostatic charges. Prototype microneedles were fabricated using a multi-layer surface micromachining process. DNA stained with a fluorescent dye (4‘, 6-DIAMIDINO-2-PHENYLINDOLE DIHYDROCHLORIDE or DAPI) was visualized using fluorescent illumination as the DNA was attracted to and repelled from the tips of MEMS microneedles using a 1.5 V DC source. The pumpless MEMS microneedle represents an important and significant step in the development of a self-contained, automated, MEMS-based microinjection system.
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Youoku, Sachihiro, Yoshinori Suto, Moritoshi Ando, and AkioIto Popp. "Automated microinjection system for adherent cells." In European Conference on Biomedical Optics. Washington, D.C.: OSA, 2007. http://dx.doi.org/10.1364/ecbo.2007.6633_27.

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Youoku, Sachihiro, Yoshinori Suto, Moritoshi Ando, and Akio Ito. "Automated microinjection system for adherent cells." In European Conference on Biomedical Optics, edited by Jürgen Popp and Gert von Bally. SPIE, 2007. http://dx.doi.org/10.1117/12.728081.

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Sarvi, Masoud Nasiri, and M. T. Ahmadian. "Application of a New Spherical Super Element in Predicting the Deformation of Biological Cells in Microinjection." In ASME 2011 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/detc2011-47653.

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Biological cell injection is a sensitive and important work which is implemented in injection of foreign materials into individual cells. Microinjection is significantly developed in the field of drug discovery and genetics so predicting the behavior of cell in microinjection is remarkably important because a tiny excessive manipulation force can destroy the tissue of the biological cell. There are a few analytical methods available to simulate the cell injection, hence the numerical methods such as FEM are suitable to be used to model the microinjection. In this study, a new spherical super element is presented to model the biological cells and deformation of a specific cell under an external force is performed. The relationship between the injection force and the deformation of biological cell is demonstrated by using super element formulations. For validating the model, results are compared with findings of analytical and experimental methods. The advantage of this element is that only a few super elements can predict the static behavior of biological cell in microinjection properly instead of implementing a large number of conventional elements, so using the super element to model the cell can decrease the run time with suitable accuracy.
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Sarvi, Masoud Nasiri, and M. T. Ahmadian. "Comparison of Deformation Analysis of a Biological Cell Under an Injection Force Using Analytical, Experimental and Finite Element Methods and Artificial Neural Network." In ASME 2011 International Mechanical Engineering Congress and Exposition. ASMEDC, 2011. http://dx.doi.org/10.1115/imece2011-63791.

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Biological cell injection is a sensitive and important work which is implemented in injection of foreign materials into individual cells. Microinjection is significantly developed in the field of drug discovery and genetics so predicting the behavior of cell in microinjection is remarkably important because a tiny excessive manipulation force can destroy the tissue of the biological cell. There are a few analytical methods available to simulate the cell injection, hence the numerical methods such as FEM are suitable to be used to model the microinjection. In this study, a new spherical super element is presented to model the biological cells and deformation of a specific cell under an external force is performed. The relationship between the injection force and the deformation of biological cell is demonstrated by using super element formulations. For validating the model, results are compared with findings of analytical and experimental methods and an Artificial Neural Network (ANN) model. The advantage of this element is that only a few super elements can predict the static behavior of biological cell in microinjection properly instead of implementing a large number of conventional elements, so using the super element to model the cell can decrease the run time with suitable accuracy.
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Peng, Cheng, Ingrid Wilke, and Robert E. Palazzo. "Microinjection by femtosecond near-infrared laser pulses." In Frontiers in Optics. Washington, D.C.: OSA, 2004. http://dx.doi.org/10.1364/fio.2004.fwf3.

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Becattini, Gabriele, Leonardo S. Mattos, and Darwin G. Caldwell. "Anisotropic Contour Completion for Cell Microinjection Targeting." In 2010 20th International Conference on Pattern Recognition (ICPR). IEEE, 2010. http://dx.doi.org/10.1109/icpr.2010.554.

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Mattos, Leonardo, Edward Grant, and Randy Thresher. "Speeding Up Video Processing for Blastocyst Microinjection." In 2006 IEEE/RSJ International Conference on Intelligent Robots and Systems. IEEE, 2006. http://dx.doi.org/10.1109/iros.2006.282395.

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Lu, Zhe, Peter C. Y. Chen, Joo Hoo Nam, Ruowen Ge, and Wei Lin. "A Micromanipulation System for Automatic Batch Microinjection." In 2007 IEEE International Conference on Robotics and Automation. IEEE, 2007. http://dx.doi.org/10.1109/robot.2007.363953.

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Youhua Tan, Dong Sun, and Wenhao Huang. "A mechanical model of biological cells in microinjection." In 2008 IEEE International Conference on Robotics and Biomimetics. IEEE, 2009. http://dx.doi.org/10.1109/robio.2009.4912980.

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Reports on the topic "Microinjection"

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Chandler, D. P., M. Welt, and F. C. Leung. Development of a rapid and efficient microinjection technique for gene insertion into fertilized salmonid eggs. Office of Scientific and Technical Information (OSTI), October 1990. http://dx.doi.org/10.2172/6214172.

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