Relevant bibliographies by topics / Microfluidic technique

Academic literature on the topic 'Microfluidic technique'

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Published: 10 December 2022
Last updated: 29 January 2023

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Journal articles on the topic "Microfluidic technique"

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Marzban, Mostapha, Ehsan Yazdanpanah Moghadam, Javad Dargahi, and Muthukumaran Packirisamy. "Microfabrication Bonding Process Optimization for a 3D Multi-Layer PDMS Suspended Microfluidics." Applied Sciences 12, no. 9 (May 4, 2022): 4626. http://dx.doi.org/10.3390/app12094626.

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Microfluidic systems have received increased attention due to their wide variety of applications, from chemical sensing to biological detection to medical analysis. Microfluidics used to be fabricated by using etching techniques that required cleanroom and aggressive chemicals. However, another microfluidic fabrication technique, namely, soft lithography, is less expensive and safer compared to former techniques. Polydimethylsiloxane (PDMS) has been widely employed as a fabrication material in microfluidics by using soft lithography as it is transparent, soft, bio-compatible, and inexpensive. In this study, a 3D multi-layer PDMS suspended microfluidics fabrication process using soft lithography is presented, along with its manufacturing issues that may deteriorate or compromise the microsystem’s test results. The main issues considered here are bonding strength and trapped air-bubbles, specifically in multi-layer PDMS microfluidics. In this paper, these two issues have been considered and resolved by optimizing curing temperature and air-vent channel integration to a microfluidic platform. Finally, the suspended microfluidic system has been tested in various experiments to prove its sensitivity to different fluids and flow rates.
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Lu, Jin, Jiushen Pang, Ying Chen, Qi Dong, Jiahao Sheng, Yong Luo, Yao Lu, Bingcheng Lin, and Tingjiao Liu. "Application of Microfluidic Chips in Separation and Analysis of Extracellular Vesicles in Liquid Biopsy for Cancer." Micromachines 10, no. 6 (June 11, 2019): 390. http://dx.doi.org/10.3390/mi10060390.

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Extracellular vesicles (EVs) are becoming a promising biomarker in liquid biopsy of cancer. Separation EV from cell culture medium or biofluids with high purity and quality remains a technique challenge. EV manipulation techniques based on microfluidics have been developed in the last decade. Microfluidic-based EV separation techniques developed so far can be classified into two categories: surface biomarker-dependent and size-dependent approaches. Microfluidic techniques allow the integration of EV separation and analysis on a single chip. Integrated EV separation and on-chip analysis have shown great potential in cancer diagnosis and monitoring treatment of responses. In this review, we discuss the development of microfluidic chips for EV separation and analysis. We also detail the clinical application of these microfluidic chips in the liquid biopsy of various cancers.
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Ballacchino, Giulia, Edward Weaver, Essyrose Mathew, Rossella Dorati, Ida Genta, Bice Conti, and Dimitrios A. Lamprou. "Manufacturing of 3D-Printed Microfluidic Devices for the Synthesis of Drug-Loaded Liposomal Formulations." International Journal of Molecular Sciences 22, no. 15 (July 28, 2021): 8064. http://dx.doi.org/10.3390/ijms22158064.

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Microfluidic technique has emerged as a promising tool for the production of stable and monodispersed nanoparticles (NPs). In particular, this work focuses on liposome production by microfluidics and on factors involved in determining liposome characteristics. Traditional fabrication techniques for microfluidic devices suffer from several disadvantages, such as multistep processing and expensive facilities. Three-dimensional printing (3DP) has been revolutionary for microfluidic device production, boasting facile and low-cost fabrication. In this study, microfluidic devices with innovative micromixing patterns were developed using fused deposition modelling (FDM) and liquid crystal display (LCD) printers. To date, this work is the first to study liposome production using LCD-printed microfluidic devices. The current study deals with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes with cholesterol (2:1) prepared using commercial and 3D-printed microfluidic devices. We evaluated the effect of microfluidic parameters, chip manufacturing, material, and channel design on liposomal formulation by analysing the size, PDI, and ζ-potential. Curcumin exhibits potent anticancer activity and it has been reported that curcumin-loaded liposomes formulated by microfluidics show enhanced encapsulation efficiency when compared with other reported systems. In this work, curcumal liposomes were produced using the developed microfluidic devices and particle sizing, ζ-potential, encapsulation efficiency, and in vitro release studies were performed at 37 °C.
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Lundy, Terence. "Advanced Confocal Microscopy An Essential Technique for Microfluidics Development." Microscopy Today 14, no. 1 (January 2006): 8–13. http://dx.doi.org/10.1017/s1551929500055127.

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Many believe that microfluidics has the potential to do for chemistry and biology what the integrated circuit has done for electronics — integrating tremendously complex chemical and biological processes into simple easy-to-use devices that will eventually pervade our lives. While microfluidics has made great progress in the last decade — addressing many of the fundamental questions related to manipulating nanoliter volumes of chemicals and solutions — it still faces some very basic challenges as it moves out of the laboratory and into use. Perhaps most basic is the need for fast, accurate characterization of the size and shape of the microfluidic devices themselves. Conventional imaging and measurement techniques have proven adequate for initial development, but are unable to provide the speed and accuracy needed to support the continued development of microfluidic technologies.
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Chiesa, Enrica, Rossella Dorati, Silvia Pisani, Bice Conti, Gloria Bergamini, Tiziana Modena, and Ida Genta. "The Microfluidic Technique and the Manufacturing of Polysaccharide Nanoparticles." Pharmaceutics 10, no. 4 (December 9, 2018): 267. http://dx.doi.org/10.3390/pharmaceutics10040267.

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The microfluidic technique has emerged as a promising tool to accelerate the clinical translation of nanoparticles, and its application affects several aspects, such as the production of nanoparticles and the in vitro characterization in the microenvironment, mimicking in vivo conditions. This review covers the general aspects of the microfluidic technique and its application in several fields, such as the synthesis, recovering, and samples analysis of nanoparticles, and in vitro characterization and their in vivo application. Among these, advantages in the production of polymeric nanoparticles in a well-controlled, reproducible, and high-throughput manner have been highlighted, and detailed descriptions of microfluidic devices broadly used for the synthesis of polysaccharide nanoparticles have been provided. These nanoparticulate systems have drawn attention as drug delivery vehicles over many years; nevertheless, their synthesis using the microfluidic technique is still largely unexplored. This review deals with the use of the microfluidic technique for the synthesis of polysaccharide nanoparticles; evaluating features of the most studied polysaccharide drug carriers, such as chitosan, hyaluronic acid, and alginate polymers. The critical assessment of the most recent research published in literature allows us to assume that microfluidics will play an important role in the discovery and clinical translation of nanoplatforms.
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Ahmed, Isteaque, Katherine Sullivan, and Aashish Priye. "Multi-Resin Masked Stereolithography (MSLA) 3D Printing for Rapid and Inexpensive Prototyping of Microfluidic Chips with Integrated Functional Components." Biosensors 12, no. 8 (August 17, 2022): 652. http://dx.doi.org/10.3390/bios12080652.

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Stereolithography based 3D printing of microfluidics for prototyping has gained a lot of attention due to several advantages such as fast production, cost-effectiveness, and versatility over traditional photolithography-based microfabrication techniques. However, existing consumer focused SLA 3D printers struggle to fabricate functional microfluidic devices due to several challenges associated with micron-scale 3D printing. Here, we explore the origins and mechanism of the associated failure modes followed by presenting guidelines to overcome these challenges. The prescribed method works completely with existing consumer class inexpensive SLA printers without any modifications to reliably print PDMS cast microfluidic channels with channel sizes as low as ~75 μm and embedded channels with channel sizes as low ~200 μm. We developed a custom multi-resin formulation by incorporating Polyethylene glycol diacrylate (PEGDA) and Ethylene glycol polyether acrylate (EGPEA) as the monomer units to achieve micron sized printed features with tunable mechanical and optical properties. By incorporating multiple resins with different mechanical properties, we were able to achieve spatial control over the stiffness of the cured resin enabling us to incorporate both flexible and rigid components within a single 3D printed microfluidic chip. We demonstrate the utility of this technique by 3D printing an integrated pressure-actuated pneumatic valve (with flexible cured resin) in an otherwise rigid and clear microfluidic device that can be fabricated in a one-step process from a single CAD file. We also demonstrate the utility of this technique by integrating a fully functional finger-actuated microfluidic pump. The versatility and accessibility of the demonstrated fabrication method have the potential to reduce our reliance on expensive and time-consuming photolithographic techniques for microfluidic chip fabrication and thus drastically lowering our barrier to entry in microfluidics research.
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Zhu, Zhiyuan, Fan Zeng, Zhihua Pu, and Jiyu Fan. "Conversion Electrode and Drive Capacitance for Connecting Microfluidic Devices and Triboelectric Nanogenerator." Electronics 12, no. 3 (January 19, 2023): 522. http://dx.doi.org/10.3390/electronics12030522.

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Microfluidics is a technique that uses channels of tiny sizes to process small amounts of fluid, which can be used in biochemical detection, information technology, and other fields. In the process of microfluidic development, there are many problems that need to be solved urgently. Many microfluidic systems require the support of external devices, which increases the construction cost, and the electronic interface technology is not mature. A triboelectric nanogenerator (TENG) can harvest mechanical energy and turn it into electrical energy. It has been greatly developed now and is widely used in various fields. Nowadays, many studies are committed to the study of TENGs and microfluidic systems. The microfluidics device can be combined with a TENG to convert fluid mechanical signals into electrical signals for transmission. Meanwhile, TENGs can also act as a high-voltage source to drive microfluidic motion. In this paper, we reviewed the development of microfluidics and related technologies of microfluidic systems in conjunction with TENGs and discussed the form of electronic interface between microfluidic systems and TENG devices.
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Sánchez Barea, Joel, Juhwa Lee, and Dong-Ku Kang. "Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology." Micromachines 10, no. 6 (June 20, 2019): 412. http://dx.doi.org/10.3390/mi10060412.

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Recently, droplet-based microfluidic systems have been widely used in various biochemical and molecular biological assays. Since this platform technique allows manipulation of large amounts of data and also provides absolute accuracy in comparison to conventional bioanalytical approaches, over the last decade a range of basic biochemical and molecular biological operations have been transferred to drop-based microfluidic formats. In this review, we introduce recent advances and examples of droplet-based microfluidic techniques that have been applied in biochemistry and molecular biology research including genomics, proteomics and cellomics. Their advantages and weaknesses in various applications are also comprehensively discussed here. The purpose of this review is to provide a new point of view and current status in droplet-based microfluidics to biochemists and molecular biologists. We hope that this review will accelerate communications between researchers who are working in droplet-based microfluidics, biochemistry and molecular biology.
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Al-Amin, MD, Federica Bellato, Francesca Mastrotto, Mariangela Garofalo, Alessio Malfanti, Stefano Salmaso, and Paolo Caliceti. "Dexamethasone Loaded Liposomes by Thin-Film Hydration and Microfluidic Procedures: Formulation Challenges." International Journal of Molecular Sciences 21, no. 5 (February 26, 2020): 1611. http://dx.doi.org/10.3390/ijms21051611.

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Liposomes have been one of the most exploited drug delivery systems in recent decades. However, their large-scale production with low batch-to-batch differences is a challenge for industry, which ultimately delays the clinical translation of new products. We have investigated the effects of formulation parameters on the colloidal and biopharmaceutical properties of liposomes generated with a thin-film hydration approach and microfluidic procedure. Dexamethasone hemisuccinate was remotely loaded into liposomes using a calcium acetate gradient. The liposomes produced by microfluidic techniques showed a unilamellar structure, while the liposomes produced by thin-film hydration were multilamellar. Under the same remote loading conditions, a higher loading capacity and efficiency were observed for the liposomes obtained by microfluidics, with low batch-to-batch differences. Both formulations released the drug for almost one month with the liposomes prepared by microfluidics showing a slightly higher drug release in the first two days. This behavior was ascribed to the different structure of the two liposome formulations. In vitro studies showed that both formulations are non-toxic, associate to human Adult Retinal Pigment Epithelial cell line-19 (ARPE-19) cells, and efficiently reduce inflammation, with the liposomes obtained by the microfluidic technique slightly outperforming. The results demonstrated that the microfluidic technique offers advantages to generate liposomal formulations for drug-controlled release with an enhanced biopharmaceutical profile and with scalability.
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Hu, Beiyu, Bingxue Xu, Juanli Yun, Jian Wang, Bingliang Xie, Caiming Li, Yanghuan Yu, et al. "High-throughput single-cell cultivation reveals the underexplored rare biosphere in deep-sea sediments along the Southwest Indian Ridge." Lab on a Chip 20, no. 2 (2020): 363–72. http://dx.doi.org/10.1039/c9lc00761j.

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Dissertations / Theses on the topic "Microfluidic technique"

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DIVAKAR, RAMGOPAL. "ROOM TEMPERATURE ADHESIVE BONDING TECHNIQUE FOR MICROFLUIDIC BIOCHIPS." University of Cincinnati / OhioLINK, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1027950500.

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Owens, Tracie LeeAnne. "Engineering amphiphilic fabrics for microfluidic applications." Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/42908.

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Woven textile fabrics were designed and constructed from hydrophilic and hydrophobic spun yarns to give planar substrates containing amphiphilic microchannels with defined orientations and locations. Polypropylene fibers were spun to give hydrophobic yarns, and the hydrophilic yarns were spun from a poly(ethylene terephthalate) copolyester. Water wicking rates into the fabrics were measured by video microscopy and longitudinal wicking tests from single drops and from reservoirs. Intra-yarn microchannels in the hydrophilic polyester yarns were shown to selectively transport aqueous fluids, with the flow path governed by the placement of the hydrophilic yarns in the fabric. Simultaneous wicking of an aqueous and hydrocarbon fluid into the hydrophilic and hydrophobic microchannels of an amphiphilic fabric was successfully demonstrated. The high degree of interfacial contact and micron-scale diffusion lengths of such co-flowing immiscible fluid streams inside amphiphilic fabrics suggest potential applications as highly scalable and affordable microcontactors for industrial liquid-liquid extractions. The efficiency of liquid-liquid extractions carried out with the amphiphilic fabrics was evaluated. Solvent extraction efficiencies were shown to reach up to ~98%.
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Li, Haifeng. "An evanescent-wave based particle image velocimetry technique." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/26472.

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Thesis (Ph. D.)--Mechanical Engineering, Georgia Institute of Technology, 2009.
Committee Chair: Yoda, Minami; Committee Member: Aidun, Cyrus; Committee Member: Breedveld, Victor; Committee Member: Fedorov, Andrei; Committee Member: Zhu, Cheng. Part of the SMARTech Electronic Thesis and Dissertation Collection.
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Rabehi, Amine. "Electromagnetic microsystem for the detection of magnetic nanoparticles in a microfluidic structure for immunoassays." Thesis, Sorbonne université, 2018. http://www.theses.fr/2018SORUS129/document.

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La détection et quantification d’agent biologique occupe une place prépondérante dans la prévention et la détection des dangers possibles pour la santé publique (épidémie ou pandémie), l’environnement ainsi que d’autres risques contextuelles (bioterrorisme, armes biologique ou chimiques…etc.). Par conséquent, le développement d’un système portable et à moindre coût permettant de détecter ces dangers constitue l’axe de recherche pluridisciplinaire de la collaboration entre différents laboratoires de l’UPMC (Paris 6) et « RWTH university » à Aachen en Allemagne. Dans ce projet, nous avons étudié les aspects pluridisciplinaires d’un microsystème (LoC) électromagnétique de détection immunologique basé sur l’utilisation de nanoparticules magnétiques (MNP). En raison de leur extractabilité et de leur triabilité, les MNP sont adaptées à l'examen d'échantillons biologiques, servant de marqueurs pour des réactions biochimiques. La plupart des techniques classiques de détection existantes sont basées sur des méthodes colorimétrique, fluorescence ou électrochimique qui souffrent en majorité de problème de temps d’analyse et de sensibilité. A cet égard, Les méthodes d’immuno-détection magnétiques constituent une alternative prometteuse. Cette détection est effectuée à l’aide des MNP qui sont spécifiquement bio-fonctionnalisés en surface afin d’être liée à la cible (virus, anticorps…etc). La nouvelle méthode magnétique de mélange de fréquence permet la détection et la quantification de ces MNP avec une grande dynamique. Dans cette thèse, l’effort est dirigé vers la miniaturisation de ce système. Pour ce faire, nous avons développé un ensemble d’outils analytiques et de simulations multiphysiques afin d’optimiser les dimensions des parties électromagnétique (bobines planaires) et microfluidiques. Par la suite, des prototypes de cette structure de détection à partir de bobines en circuits imprimés et de réservoirs microfluidiques en PDMS sont dimensionnés et réalisés. Les performances de ces prototypes ont été évaluées en termes de limite de détection de MNP, linéarité et plage dynamique. En outre, ces prototypes ont permis de valider les outils de dimensionnement réalisés. Une limite de détection de nanoparticules magnétiques de 15ng/mL a été mesurée avec un volume d'échantillon de 14 μL correspondant à une goutte de sang. Finalement, la validation du système quant à l’immuno-détection est abordée avec un état de l’art et le développement d’une procédure de fonctionnalisation biochimique de surface ainsi que des premiers tests pour sa validation
The detection and quantification of a biological agent or entity has become paramount to anticipate a possible health threat (epidemic or pandemic), environmental threat or to combat other contextual threats (bioterrorism, chemical and biological weapons, drugs). Consequently, developing a portable cost effective device that could detect and quantify such threats is the research focus of the joint multidisciplinary project between UPMC (Paris 6) laboratories and RWTH university in Aachen, Germany. In the framework of this project, we have studied the multidisciplinary aspects of an electromagnetic microsystem for immunologic detection based on magnetic nanoparticles (MNP) in a microfluidic lab-on-chip (LoC). Because of their extractability and sortability, magnetic nanoparticles are adapted for examination of biological samples, serving as markers for biochemical reactions. So far, the final detection step is mostly achieved by well-known immunochemical or fluorescence-based techniques which are time consuming and have limited sensitivity. Therefore, magnetic immunoassays detecting the analyte by means of magnetic markers constitute a promising alternative. MNP covered with biocompatible surface coating can be specifically bound to analytes, cells, viruses or bacteria. They can also be used for separation and concentration enhancement. The novel frequency mixing magnetic detection method allows quantifying magnetic nanoparticles with a very large dynamic measurement range. In this thesis, emphasis is put on the miniaturized implementation of this detection scheme. Following the development of analytical and multiphysics simulations tools for optimization of both excitation frequencies and detection planar coils, first multilayered printed circuit board prototypes integrating all three different coils along with an adapted microfluidic chip has been designed and realized. These prototypes have been tested and characterized with respect to their performance for limit of detection (LOD) of MNP, linear response and validation of theoretical concepts. Using the frequency mixing magnetic detection technique, a LOD of 15ng/mL for 20 nm core sized MNP has been achieved with a sample volume of 14 μL corresponding to a drop of blood. Preliminary works for biosensing have also been achieved with a state of the art of surface functionalization and a developed proposed biochemical immobilization procedure and preliminary tests of its validation
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Johnson, Chrisopher W. A. "Design and development of a site specific protein patterning technique for use in a microfluidic antibody separation device." Thesis, University of Southampton, 2010. https://eprints.soton.ac.uk/157341/.

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The rapid quantification of the concentration of different immunoglobulins classes from patient serum is required to diagnose patients in the early stages of sepsis. Microfluidic point of care technology can improve diagnostics by decreasing the analysis time, and integrating parallel analysis in a single portable device. The design of a novel method to fabricate surfaces presenting multiple micron scale protein motifs, for integration within a microfluidic channel device, is described in this thesis. Initial research focussed on conjugating protein motifs on silicon <100> substrates in micron and submicron scale patterns. A method involving the UV-initiated conjugation of a heterobifunctional linker, undecylenic acid N-Hydroxysuccinimde ester (UANHS), to a hydrogen terminated silicon surface was investigated. A photolithographic mask and phase mask were used to form micron and submicron UANHS motifs respectively, on silicon. The conjugation of protein with UANHS motifs was investigated to determine how reproducible the patterns were. The conjugation of streptavidin, streptavidin-FITC, NeutrAvidin, single domain protein L and multidomain protein L to silicon surfaces, upon reaction with UANHS, were investigated. Fluorescently labelled probes that associated with the protein motifs were used to confirm successful conjugation of protein to the silicon. Micron scale motifs of streptavidin, streptavidin-FITC, NeutrAvidin and single domain protein L could be formed reproducibly on silicon. Using a phase mask 140 nm motifs of streptavidin-FITC, conjugated to silicon, were achieved. Also an alternative method to pattern multiple proteins onto glass surfaces was investigated. A 500,000 MW dextran was modified to incorporate an aryl azide moiety, which was subsequently immobilised on glass surfaces. A method to synthesise and characterise the aryl azide conjugated dextran was investigated, as well as methods to characterise and improve the reproducibility of the aryl azide conjugated dextran layer immobilised on the glass surface. Two photolithographic masks and glass surfaces with alignment marks were fabricated. The masks were used to form micron scale protein motifs, via a photoinitiated conjugation reaction, on the aryl azide conjugated dextran surface. An in-house alignment system was built and a method to produce adjacent protein motifs was investigated. Two adjacent micron scale patterns of multidomain protein L and protein A were achieved. The surface density of conjugated protein L was investigated and a density of ~1.16x1011 molecules/cm2 was confirmed. This approach offers a method to attach high density micron scale protein motifs, aligned with micron scale resolution, which is vital to the realisation of a microfluidic point of care device.
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Lu, Heng. "Development of droplet-based microfluidic tools for toxicology and cancer research." Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCB064.

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Ce projet de thèse portait sur le développement d’outils microfluidiques pour la toxicologie et la recherche contre le cancer. En permettant l’analyse simultanée d’un très grand nombre de réactions biologiques ou chimiques réalisés dans des compartiments indépendants (ie. gouttelettes), la microfluidique de gouttes offre une sensibilité de détection et une précision sans précédent pour l’analyse de molécules biologiques, telles que l’ADN ou les Anticorps, en comparaison des expériences réalisées conventionnellement en tubes ou en microplaques (essais en « bulk » ou volume). Ce format permet également de réaliser des expériences à très haut débit et est particulièrement pertinent pour la toxicologie, où des analyses robustes de l’effet des médicaments sont nécessaires. De même, ces procédures sont également très adaptées à l’analyse de cellules uniques pour le séquençage ADN ou ARN et l’épigénomique. Tout cela fait de la microfluidique en goutte un outil puissant pour la toxicologie et la recherche sur le cancer. En premier temps, une méthode du comptage précise des cellules encapsulée dans des microgouttelettes, nommée « hémocytométrie microfluidique », a été développée. Un nouvel algorithme de comptage a été proposé. Des cellules bactériennes (Escherichia Coli) et des cellules de 2 lignées humaines différentes (HL60 and H1975) ont été testées. Le nombre de chaque type de cellules a été déterminé avec une haute corrélation entre la théorie (basée sur la distribution de Poisson) et les résultats expérimentaux. Avec ces résultats robustes, un protocole de microfluidique en goutte a été mis en place pour interroger la viabilité cellulaire et la prolifération des 2 lignées humaines. Ces résultats sont en concordance avec ceux de la littérature. Pour la toxicologie, 3 différents modèles, y compris des microsomes (extrait de cellules d’insectes infectées par un baculovirus exprimant le cytochrome P450 3A4 humain, CYP3A4), HepG2-CYP3A4 (modifiée génétiquement pour exprimer le gène CYP3A4 humain), et HepaRG, une lignée hépatique, ont été évaluées pour l’activité enzymatique du CYP3A4, une enzyme largement utilisée en routine pour le criblage de médicament candidat. Les microsomes ont permis de développer un essai fluorogénique permettant de mesurer l’inhibition du CYP3A4. Cependant, ni l’utilisation des microsomes ni des cellules HepG2 exprimant CYP3A4 n’a donné de résultats satisfaisants en microgouttelettes. L’utilisation des cellules HepaRG, une lignée cellulaire qui conserve la majorité de l’expression des cytochromes P450 et des récepteurs nucléaires nécessaire à leur expression, a montré des résultats encourageant à la fois sur les tests de mesure de l’activité enzymatique et d’analyse de l’induction du CYP3A4. Pour la recherche sur le cancer, 4 essais originaux de PCR digitale en gouttes ont été mis en place pour la détection et la quantification de mutations (NRAS, DNMT3A, SF3B1 and JAK2) importante pour les syndromes myélodysplasiques, un groupe hétérogène de maladies touchant les cellules souches hématopoïétiques caractérisées par une hématopoïèse inefficace et des cytopénies périphériques. Finalement, un essai de PCR sur cellule unique encapsulées au sein de billes agarose a été proposé
This thesis project consists in developing droplet-based microfluidic tools for toxicology and cancer research. Owing to its large numbers of discretized volumes, sensitivity of detection of droplet-based microfluidics for biological molecules such as DNA and antibody is much higher than bulk assays. This high throughput format is particularly suitable for experiments where a robust dose-response curve is needed, as well as for single cell analysis with applications in genomic or sequencing and epigenetics. All above makes droplet-based microfluidics a powerful tool for toxicology and cancer research. In a first part of the work, an accurate cell counting method, named “microfluidics hemocytometry”, has been developed. A new counting algorithm was proposed to count the cells within each droplet. Escherichia Coli and two different human cell lines (HL60 and H1975) were used to validate our strategy. The number of each type of cells in droplets was determined with a high consistency between theory (Poisson distribution) and experimental results. With these robust results, a droplet-based microfluidic protocol has then been established to inquiry both cell viability and proliferation for the two human cell lines. The results are in good agreement with the one of the literature. For the toxicology, 3 different biological models, including microsomes (extracted from baculovirus-infected insect cell expressing human CYP3A4), HepG2-CYP3A4 (genetically modified to express the human CYP3A4 gene) and HepaRG liver cells lines were evaluated for enzymatic activity of cytochromes P450 (CYP3A4), a routinely used enzyme for drug candidate screening. Microsome-based assays were used to validate a fluorogenic inhibition assay. However neither microsome-based assay nor the assay using CYP3A4 expressing HepG2 gave satisfying results in droplet-based format. However, HepaRG cells, a hepatic function-conserved cell line with most cytochrome and related nuclear receptors, demonstrated high relevance both for enzymatic activity testing and CYP3A4 expression induction study. For cancer research, 4 different picoliter droplet-based PCR assays were developed for the detection and quantification of mutations (NRAS, DNMT3A, SF3B1 and JAK2) present in Myelodysplastic syndromes, a heterogeneous group of clonal bone marrow hematopoietic stem cell disorders characterized by ineffective hematopoiesis and peripheral cytopenias. Furthermore, a single cell multistep PCR assay using encapsulation of target DNA in agarose droplets was proposed
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Nikcevic, Irena. "Development of techniques and materials for microfluidic devices." Cincinnati, Ohio : University of Cincinnati, 2008. http://rave.ohiolink.edu/etdc/view.cgi?acc_num=ucin1212155007.

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Rajah, Luke. "Biophysical and microfluidic techniques for investigating protein aggregation." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608030.

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NIKCEVIC, IRENA. "Development of techniques and materials for microfluidic devices." University of Cincinnati / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1212155007.

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Puccetti, Giacomo <1988&gt. "Optical Techniques for Experimental Tests in Microfluidics." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2016. http://amsdottorato.unibo.it/7534/.

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This PhD dissertation deals with the use of optical, non-invasive measurement techniques for the investigation of single and two-phase flows in microchannels. Different experimental techniques are presented and the achieved results are critically discussed. Firstly, the inverse use of the micro Particle Image Velocimetry technique for the detection of the real shape of the inner cross-section of an optical accessible microchannel is shown by putting in evidence the capability of this technique to individuate the presence of singularities along the wetted perimeter of the microchannel. Then, the experimental measurement of the local fluid temperature using non-encapsulated Thermochromic Liquid Crystal particles is discussed. A deep analysis of the stability of the color of these particles when exposed to different levels of shear stress has been conducted by demonstrating that these particles can be used for simultaneous measurements of velocity and temperature in water laminar flows characterized by low Reynolds numbers (Re < 10). A preliminary experiment where the TLC thermography is coupled to the APTV method for the simultaneous measurement of the three-dimensional velocity and temperature distribution in a microchannel is shown. Finally, an experimental analysis of the different flow patterns observed for an adiabatic air-water mixture generated by means of a micro T-junction is discussed. The main air-water mixture features have been deeply observed in 195 different experimental conditions in which values of superficial velocity ranging between 0.01 m/s and 0.15 m/s for both the inlet flows (air and water) are imposed. The flow patterns of the air-water mixture are strongly influenced by the value of the water superficial velocity; on the contrary, the air superficial velocity plays a secondary role for the determination of the characteristics of the bubbles (i.e. length).
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Books on the topic "Microfluidic technique"

1

Shelley, Minteer D. Microfluidic Techniques. New Jersey: Humana Press, 2005. http://dx.doi.org/10.1385/1592599974.

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Microfluidic lab-on-a-chip for chemical and biological analysis and discovery. Boca Raton, Fla: Taylor & Francis, 2005.

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D, Minteer Shelley, ed. Microfluidic techniques: Reviews and protocols. Totowa, N.J: Humana Press, 2006.

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Li, Xiujun, and Zhou Yu. Microfluidic devices for biomedical applications. Cambridge, UK: Woodhead Publishing, 2013.

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Krishnendu, Chakrabarty, and Zeng Jun, eds. Design automation methods and tools for microfluidics-based biochips. Dordrecht: Springer, 2006.

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1982-, Xu Tao, ed. Digital microfluidic biochips: Design automation and optimization. Boca Raton: Taylor & Francis, 2010.

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Wang, Wanjun. Microfluidics, bioMEMS, and medical microsystems VII: 26-28 January 2009, San Jose, California, United States. Edited by SPIE (Society). Bellingham, Wash: SPIE, 2009.

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Cullum, Brian M., and Eric S. McLamore. Smart biomedical and physiological sensor technology IX: 26 April 2012, Baltimore, Maryland, United States. Edited by SPIE (Society). Bellingham, Wash: SPIE, 2012.

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B, Matsko Nadejda, and SpringerLink (Online service), eds. Analytical Imaging Techniques for Soft Matter Characterization. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012.

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Cheng, Ya. Microbiochips monolithically integrated with microfluidics, micromechanics, photonics, and electronics by 3D femtosecond laser direct writing. Hauppauge, N.Y: Nova Science Publishers, 2010.

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Book chapters on the topic "Microfluidic technique"

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Khnouf, Ruba, Areen Al Bashir, Ala’a Migdade, Esra’a Alshawa, and Arwa Sheyab. "Image based microfluidic mixing evaluation technique." In Proceedings of the 1st International Congress on Engineering Technologies, 91–99. London: CRC Press, 2021. http://dx.doi.org/10.1201/9781003178255-13.

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Bhattacharya, Rupam, Pranab Roy, and Hafizur Rahaman. "A New Combined Routing Technique in Digital Microfluidic Biochip." In Advances in Intelligent Systems and Computing, 441–50. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-1951-8_40.

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Wang, Yao Nan, Chiu Feng Lin, S. T. Wu, C. L. Chang, H. T. Chen, Chien Hsiung Tsai, and Lung Ming Fu. "Experimental Investigation of High-Resolution Injection Technique in Microfluidic Chips." In Materials Science Forum, 409–14. Stafa: Trans Tech Publications Ltd., 2006. http://dx.doi.org/10.4028/0-87849-990-3.409.

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Chen, Xiaodao, Yuewei Wang, Chaowei Wan, and Xiaohui Huang. "Fault Tolerance-Aware Design Technique for Cyber-Physical Digital Microfluidic Biochips." In Big Data Analytics for Cyber-Physical Systems, 203–14. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-43494-6_9.

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Nesbitt, Warwick S., Francisco J. Tovar-Lopez, Erik Westein, Ian S. Harper, and Shaun P. Jackson. "A Multimode-TIRFM and Microfluidic Technique to Examine Platelet Adhesion Dynamics." In Adhesion Protein Protocols, 39–58. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-538-5_3.

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Chowdhury, Sagarika, Rajat Kumar Pal, and Goutam Saha. "A Novel Double Fault Diagnosis and Detection Technique in Digital Microfluidic Biochips." In Computer Information Systems and Industrial Management, 181–92. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-24369-6_15.

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Yeh, Chuan-Feng, Hao-Chen Chang, and Chia-Hsien Hsu. "Dual-Well Microfluidic Technique for Single Cell Isolation and Long-Term Clonal Culture." In Handbook of Single Cell Technologies, 1–24. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-10-4857-9_26-1.

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Yeh, Chuan-Feng, Hao-Chen Chang, and Chia-Hsien Hsu. "Dual-Well Microfluidic Technique for Single Cell Isolation and Long-Term Clonal Culture." In Handbook of Single-Cell Technologies, 263–86. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-10-8953-4_26.

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Beushausen, Volker, Karsten Roetmann, Waldemar Schmunk, Mike Wellhausen, Christoph Garbe, and Bernd Jähne. "2D-Measurement Technique for Simultaneous Quantitative Determination of Mixing Ratio and Velocity Field in Microfluidic Applications." In Notes on Numerical Fluid Mechanics and Multidisciplinary Design, 155–64. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-642-01106-1_16.

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Choi, Jin-Woo, Sanghyo Kim, Ramachandran Trichur, Hyoung J. Cho, Aniruddha Puntambekar, Robert L. Cole, Jeffrey R. Simkins, et al. "A Plastic Micro Injection Molding Technique Using Replaceable Mold-Disks for Disposable Microfluidic Systems and Biochips." In Micro Total Analysis Systems 2001, 411–12. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-1015-3_181.

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Conference papers on the topic "Microfluidic technique"

1

Martel, Joseph, and Bradford A. Bruno. "Shear Stress Measurement in Microfluidic Systems: Liquid Crystal Technique." In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-68708.

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Preliminary work towards developing a liquid crystal based technique for direct shear stress measurements over solid surfaces in microfluidic systems is presented. The microfluidic slug flow study which motivated the development of this technique is presented, as is general background on microfluidics. The theory of shear sensitive liquid crystals is reviewed and then expanded upon in regards to the specific type of flow considered in this study; slug flow. A prototype apparatus is described which is capable of generating slugs, and has appropriate optical access to test the liquid crystal shear stress measurement technique. The microchannel (150μm × 250μm laser etched glass), auxiliary flow (Cole-Parmer Infusion 100 Syringe Pump), and optical data collection (Olympus BX51 microscope) subsystems are all described in detail. Procedures for applying the cholesteric liquid crystal mixtures obtained through Pressure Chemicals Ltd. to the microchannel and for collecting liquid crystal data are described as well. Finally, preliminary results are presented, the current status of the technique is stated along with proposed directions for future research work.
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de Araújo Filho, Walter Duarte, Rigoberto E. M. Morales, Fábio K. Schneider, and Luciana Martins P. de Araújo. "Microfluidics Device Manufacturing Using the Technique of 3D Printing." In ASME 2014 12th International Conference on Nanochannels, Microchannels, and Minichannels collocated with the ASME 2014 4th Joint US-European Fluids Engineering Division Summer Meeting. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/icnmm2014-21540.

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The Microfluidics emerged at the end of the 1970’s decade as a result of the use of the technology responsible for the development of micro electromechanical systems (MEMS) that utilized the infrastructure and well-established manufacturing techniques for microelectronics. Initially, silicon was used as substrate for the manufacture of micros systems, however in the last decades it has been replaced by other materials like glass, polymers and ceramic. Currently the most widely used technique in the fabrication of Microfluidic devices is the microlithography. However, besides having a high operating cost, this manufacturing technique requires additional procedures for adapting the interfaces of micro scale to macro scale (e.g. connections), which makes it even more complex. 3D printing technique used in the fabrication of microfluidic devices can overcome these difficulties and become a viable alternative, since it has the ability to fabricate devices in a single printing step. In addition to removing the need for additional procedures relating to adaptations of the interfaces, this technique allows to produce devices with circular sections channels. In this work microfluidic devices are manufactured according to the technique of 3D printing. They were tested for the production of monodisperse microbubbles aimed to clinical applications. The results proved the efficiency of the devices in the generation of microbubbles with the percentage variation rate of 0.4% and average diameter of 73.7 μm.
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Bachman, Mark, Yuh-Min Chiang, Charles Y. Chu, and Guann-pyng Li. "Laminated microfluidic structures using a micromolding technique." In Symposium on Micromachining and Microfabrication, edited by Chong H. Ahn and A. Bruno Frazier. SPIE, 1999. http://dx.doi.org/10.1117/12.359331.

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Roy, Pranab, Hafizur Rahaman, and Parthasarathi Dasgupta. "A layout based customized testing technique for total microfluidic operations in digital microfluidic biochips." In 2014 IEEE 17th International Symposium on Design and Diagnostics of Electronic Circuits & Systems (DDECS). IEEE, 2014. http://dx.doi.org/10.1109/ddecs.2014.6868775.

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Tzeng, Yung-Chin, Yueh-Jen Chen, Chang Chuan, Li-Chen Pan, and Fan-Gang Tseng. "Microfluidic devices for aiding in-vitro fertilization technique." In 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). IEEE, 2017. http://dx.doi.org/10.1109/nems.2017.8016993.

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Pao, Sung-Yen, Shih-Jie Lo, Kai-Yuan Tang, Stevel Hsu, and Da-Jeng Yao. "Cell Detection in Microfluidic System by Terahertz Technique." In 2018 IEEE 13th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). IEEE, 2018. http://dx.doi.org/10.1109/nems.2018.8556986.

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Gountia, Debasis, and Sudip Roy. "Design-for-Trust Technique for Microfluidic Biochip Layout." In 2019 IEEE Region 10 Symposium (TENSYMP). IEEE, 2019. http://dx.doi.org/10.1109/tensymp46218.2019.8971286.

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Kim, Joohyun, and Jungchul Lee. "Development of microfluidic resonators via silicon-on-nothing technique." In 2015 28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2015. http://dx.doi.org/10.1109/memsys.2015.7050917.

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Mukherjee, Subhamita, Indrajit Banerjee, and Tuhina Samanta. "Defect aware droplet routing technique in digital microfluidic biochip." In 2014 IEEE International Advance Computing Conference (IACC). IEEE, 2014. http://dx.doi.org/10.1109/iadcc.2014.6779290.

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Baig, Sarfaraz, Bing Chen, Angel Flores, Sangyup Song, and Michael R. Wang. "Channel waveguide fabrication via microtransfer molding and microfluidic technique." In SPIE OPTO: Integrated Optoelectronic Devices, edited by Alexei L. Glebov and Ray T. Chen. SPIE, 2009. http://dx.doi.org/10.1117/12.810554.

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