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Journal articles on the topic 'Microfluidic Probe Integrated Device'

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Author: Grafiati

Published: 10 December 2022

Last updated: 28 January 2023

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1

Shinha, Kenta, and Hiroshi KIMURA. "Microfluidic probe integrated device for cell-based assay." Proceedings of Mechanical Engineering Congress, Japan 2016 (2016): J0270202. http://dx.doi.org/10.1299/jsmemecj.2016.j0270202.

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2

Shinha, Kenta, Wataru Nihei, and Hiroshi Kimura. "A Microfluidic Probe Integrated Device for Spatiotemporal 3D Chemical Stimulation in Cells." Micromachines 11, no. 7 (July 16, 2020): 691. http://dx.doi.org/10.3390/mi11070691.

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Numerous in vitro studies have been conducted in conventional static cell culture systems. However, most of the results represent an average response from a population of cells regardless of their local microenvironment. A microfluidic probe is a non-contact technology that has been widely used to perform local chemical stimulation within a restricted space, providing elaborated modulation and analysis of cellular responses within the microenvironment. Although microfluidic probes developed earlier have various potential applications, the two-dimensional structure can compromise their functionality and flexibility for practical use. In this study, we developed a three-dimensional microfluidic probe integrated device equipped with vertically oriented microchannels to overcome crucial challenges and tested the potential utility of the device in biological research. We demonstrated that the device tightly regulated spatial diffusion of a fluorescent molecule, and the flow profile predicted by simulation replicated the experimental results. Additionally, the device modulated the physiological Ca2+ response of cells within the restricted area by altering the local and temporal concentrations of biomolecules such as ATP. The novel device developed in this study may provide various applications for biological studies and contribute to further understanding of molecular mechanisms underlying cellular physiology.

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3

Sakuma, Shinya, and Fumihito Arai. "Cellular Force Measurement Using a Nanometric-Probe-Integrated Microfluidic Chip with a Displacement Reduction Mechanism." Journal of Robotics and Mechatronics 25, no. 2 (April 20, 2013): 277–84. http://dx.doi.org/10.20965/jrm.2013.p0277.

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This paper presents noncontact nanometric positioning of a probe tip with high output force in a microfluidic chip. To measure cellular force in a microfluidic chip on the basis of cell deformation, we employed an on-chip probe with a magnetic drive method with actuation on the order of millinewtons. A reduction mechanism was proposed to realize nanometric resolution for positioning the probe tip. This mechanism utilizes a combination of springs with different stiffness levels and is driven bymagnetic force. The performance of the prototype device was examined and results indicated that, as ameasure of repetitive positioning accuracy, standard deviation of probe tip displacement was under 0.18 µm. Deformation was successfully measured for an oocyte on the order of 0.1 mN, demonstrating, as a consequence, nanometric order noncontact actuation of the on-chip probe with high output force. Using this on-chip probe, cellular force measurement was achieved for the microfluidic chip.

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4

Zamboni, Riccardo, Annamaria Zaltron, Elena Izzo, Gregorio Bottaro, Davide Ferraro, and Cinzia Sada. "Opto-Microfluidic System for Absorbance Measurements in Lithium Niobate Device Applied to pH Measurements." Sensors 20, no. 18 (September 19, 2020): 5366. http://dx.doi.org/10.3390/s20185366.

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The aim of Lab-on-a-chip systems is the downscaling of analytical protocols into microfluidic devices, including optical measurements. In this context, the growing interest of the scientific community in opto-microfluidic devices has fueled the development of new materials. Recently, lithium niobate has been presented as a promising material for this scope, thanks to its remarkable optical and physicochemical properties. Here, we present a novel microfluidic device realized starting from a lithium niobate crystal, combining engraved microfluidic channels with integrated and self-aligned optical waveguides. Notably, the proposed microfabrication strategy does not compromise the optical coupling between the waveguides and the microchannel, allowing one to measure the transmitted light through the liquid flowing in the channel. In addition, the device shows a high versatility in terms of the optical properties of the light source, such as wavelength and polarization. Finally, the developed opto-microfluidic system is successfully validated as a probe for real-time pH monitoring of the liquid flowing inside the microchannel, showing a high integrability and fast response.

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5

Horayama, Masayuki, Tomoki Ohkubo, Kenta Arai, Kazuya Kabayama, Teruo Fuji, and Hiroshi Kimura. "H-2-4 Cell-based assay device integrated with a microfluidic probe." Proceedings of the Conference on Information, Intelligence and Precision Equipment : IIP 2014 (2014): _H—2–4–1_—_H—2–4–2_. http://dx.doi.org/10.1299/jsmeiip.2014._h-2-4-1_.

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6

Horayama, Masayuki, Tomoki Okubo, Teruo Fujii, and Hiroshi Kimura. "5PM1-C-3 Cell-based assay device integrated with a microfluidic probe." Proceedings of the Symposium on Micro-Nano Science and Technology 2013.5 (2013): 29–30. http://dx.doi.org/10.1299/jsmemnm.2013.5.29.

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7

Caneira, Catarina R. F., Denis R. Santos, Virginia Chu, and João P. Conde. "Regenerable Bead-Based Microfluidic Device with integrated THIN-Film Photodiodes for Real Time Monitoring of DNA Detection." Proceedings 2, no. 13 (December 10, 2018): 953. http://dx.doi.org/10.3390/proceedings2130953.

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Nanoporous microbead-based microfluidic systems for biosensing applications allow enhanced sensitivities, while being low cost and amenable for miniaturization. The regeneration of the microfluidic biosensing system results in a further decrease in costs while the integration of on-chip signal transduction enhances portability. Here, we present a regenerable bead-based microfluidic device, with integrated thin-film photodiodes, for real-time monitoring of molecular recognition between a target DNA and complementary DNA (cDNA). High-sensitivity assay cycles could be performed without significant loss of probe DNA density and activity, demonstrating the potential for reusability, portability and reproducibility of the system.

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8

Lee, Ji Hye, June Moon Jang, Han Sang Cho, Ki Cheol Han, Tae Song Kim, Ji Yoon Kang, and Eun Gyeong Yang. "Design and Characterization of Microfluidic Analysis System for RNA-Aminoglycoside Interactions." Key Engineering Materials 277-279 (January 2005): 90–95. http://dx.doi.org/10.4028/www.scientific.net/kem.277-279.90.

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Microfluidic devices are of considerable interest, since such technology offers great promise for the development of powerful and versatile miniaturized analyzers. Accordingly, the present work describes a microfluidic screening system that is composed of a microchip, hydrodynamic pumping unit and fluorescence detectors. To develop an assay for RNA-aminoglycoside interactions, microchips are designed and fabricated on a glass substrate, then flow simulations are performed in the microchannels. After optimizing the flow control and buffer composition for fluorescence-based biochemical assays, a fluorescently labeled aminoglycoside probe and RNA are allowed to flow continuously to the main micro-channel based on hydrodynamic pumping and their interactions monitored by fluorescence quenching, which is reversed upon competition with other aminoglycosides. Consequently, the proposed device can serve as an integrated microfluidic platform for the high-throughput screening of high affinity antibiotics for RNA targets.

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Hui, Liu, Tang, Song, Madou, Xia, and Wu. "Determination of Mercury(II) on A Centrifugal Microfluidic Device Using Ionic Liquid Dispersive Liquid−Liquid Microextraction." Micromachines 10, no. 8 (August 8, 2019): 523. http://dx.doi.org/10.3390/mi10080523.

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An integrated centrifugal microfluidic device was developed to preconcentrate and detect hazardous mercury (II) in water with ionic liquid as environmentally friendly extractant. An automatically salt-controlled ionic liquid dispersive liquid–liquid microextraction on a centrifugal microfluidic device was designed, fabricated, and characterized. The entire liquid transport mixing and separation process was controlled by rotation speed, siphon valves, and capillary valves. Still frame images on the rotating device showed the process in detail, revealing the sequential steps of mixing, siphon priming, transportation between chambers, and phase separation. The preconcentration of red dye could be clearly observed with the naked eye. By combining fluorescence probe and microscopy techniques, the device was tested to determine ppb-level mercury (II) in water, and was found to exhibit good linearity and low detection limit.

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10

SHINHA, Kenta, and Hiroshi KIMURA. "Evaluation of cell response to spatiotemporal chemical stimulation using a microfluidic probe integrated device." Proceedings of Mechanical Engineering Congress, Japan 2018 (2018): J0220206. http://dx.doi.org/10.1299/jsmemecj.2018.j0220206.

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11

Mansor, Muhammad Asraf, Masaru Takeuchi, Masahiro Nakajima, Yasuhisa Hasegawa, and Mohd Ridzuan Ahmad. "A novel integrated dual microneedle-microfluidic impedance flow cytometry for cells detection in suspensions." International Journal of Electrical and Computer Engineering (IJECE) 7, no. 3 (June 1, 2017): 1513. http://dx.doi.org/10.11591/ijece.v7i3.pp1513-1521.

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In this study, a new, simple and cost-effective impedance detection of yeast cell concentration by using a novel integrated dual microneedle-microfluidic impedance flow cytometry was introduced. The reported method for impedance flow cytometry detection utilizes embedded electrode and probe in the microfluidic device to perform measurement of electrical impedance when a presence of cells at sensing area. Nonetheless, this method requires costly and complicatedly fabrication process of electrode. Furthermore, to reuse the fabricated electrode, it also requires intensive and tedious cleaning process. Due to that, a dual microneedle integrated at the half height of the microchannel for cell detection as well as for electrical measurement was demonstrated. A commercial available Tungsten needle was utilized as a dual microneedle. The microneedle was easy to be removed from the disposable PDMS microchannel and can be reused with the simple cleaning process, such as washed by using ultrasonic cleaning. Although this device was low cost, it preserves the core functionality of the sensor, which is capable of detecting the passing cells at sensing area. Therefore, this device is suitable for low cost medical and food safety screening and testing process in developing countries.

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12

Palmieri, M., T. Barbuzzi, A. Maierna, M. Marchi, G. Montalbano, G. Panvini, T. Rodenfels, and W. Stoeters. "Advanced microfluidic packaging for molecular diagnostics." International Symposium on Microelectronics 2010, no. 1 (January 1, 2010): 000036–41. http://dx.doi.org/10.4071/isom-2010-ta2-paper1.

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STMicroelectronics has teamed up with Boehringer Ingelheim microParts GmbH to develop the In-Check™ Lab-on-Chip microfluidic disposable cartridge. In-Check™ is a ST proprietary platform dedicated to the in-vitro molecular diagnostics, e.g. biological analysis based on nucleic acid targets such as DNA, RNA. Its first generation has been released to commercial applications such as virus or bacteria borne infectious diseases. The second generation described herein will further enhance the platform customer experience by means of an innovative design of its disposable component. Indeed the new format represents a substantial step forward in the system integration and easy-of-use. This advanced microfluidics package enables fully automated application protocols such as analyte and reagents input, management and disposal. In-Check™ cartridge is a plastic-based self-contained embodiment which integrates a variety of design elements and components, including liquid loading and waste reservoirs, connecting micro-channels, two sets of valves one set to tight-seal biochemical reactors and the other set for fluid routing, hydrophobic membranes, fluorescent read-out window. Such microfluidic platform married with integrated Micro Electo Mechanical System (MEMS) device and software algoritms provides a highly flexible system to run complex biological assays as RNA Reverse Transcription (RT), DNA Polimerase Chain Reaction (PCR), Probe Hybridization and Detection. This paper will present the disposable product concept, key components and functionality together with the design and manufacturing challenge.

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13

Horayama, Masayuki, Kenta Shinha, Kazuya Kabayama, Teruo Fujii, and Hiroshi Kimura. "Spatial Chemical Stimulation Control in Microenvironment by Microfluidic Probe Integrated Device for Cell-Based Assay." PLOS ONE 11, no. 12 (December 8, 2016): e0168158. http://dx.doi.org/10.1371/journal.pone.0168158.

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14

Descamps, Lucie, Marie-Charlotte Audry, Jordyn Howard, Samir Mekkaoui, Clément Albin, David Barthelemy, Léa Payen, et al. "Self-Assembled Permanent Micro-Magnets in a Polymer-Based Microfluidic Device for Magnetic Cell Sorting." Cells 10, no. 7 (July 9, 2021): 1734. http://dx.doi.org/10.3390/cells10071734.

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Magnetophoresis-based microfluidic devices offer simple and reliable manipulation of micro-scale objects and provide a large panel of applications, from selective trapping to high-throughput sorting. However, the fabrication and integration of micro-scale magnets in microsystems involve complex and expensive processes. Here we report on an inexpensive and easy-to-handle fabrication process of micrometer-scale permanent magnets, based on the self-organization of NdFeB particles in a polymer matrix (polydimethylsiloxane, PDMS). A study of the inner structure by X-ray tomography revealed a chain-like organization of the particles leading to an array of hard magnetic microstructures with a mean diameter of 4 µm. The magnetic performance of the self-assembled micro-magnets was first estimated by COMSOL simulations. The micro-magnets were then integrated into a microfluidic device where they act as micro-traps. The magnetic forces exerted by the micro-magnets on superparamagnetic beads were measured by colloidal probe atomic force microscopy (AFM) and in operando in the microfluidic system. Forces as high as several nanonewtons were reached. Adding an external millimeter-sized magnet allowed target magnetization and the interaction range to be increased. Then, the integrated micro-magnets were used to study the magnetophoretic trapping efficiency of magnetic beads, providing efficiencies of 100% at 0.5 mL/h and 75% at 1 mL/h. Finally, the micro-magnets were implemented for cell sorting by performing white blood cell depletion.

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15

Novara, Chiara, Alessandro Chiadò, Niccolò Paccotti, Silvia Catuogno, Carla Lucia Esposito, Gerolama Condorelli, Vittorio De Franciscis, Francesco Geobaldo, Paola Rivolo, and Fabrizio Giorgis. "SERS-active metal-dielectric nanostructures integrated in microfluidic devices for label-free quantitative detection of miRNA." Faraday Discussions 205 (2017): 271–89. http://dx.doi.org/10.1039/c7fd00140a.

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In this work, SERS-based microfluidic PDMS chips integrating silver-coated porous silicon membranes were used for the detection and quantitation of microRNAs (miRNAs), which consist of short regulatory non-coding RNA sequences typically over- or under-expressed in connection with several diseases such as oncogenesis. In detail, metal–dielectric nanostructures which provide noticeable Raman enhancements were functionalized according to a biological protocol, adapted and optimized from an enzyme-linked immunosorbent assay (ELISA), for the detection of miR-222. Two sets of experiments based on different approaches were designed and performed, yielding a critical comparison. In the first one, the labelled target miRNA is revealed through hybridization to a complementary thiolated DNA probe, immobilized on the silver nanoparticles. In the second one, the probe is halved into shorter strands (half1 and half2) that interact with the complementary miRNA in two steps of hybridization. Such an approach, taking advantage of the Raman labelling of half2, provides a label-free analysis of the target. After suitable optimisation of the procedures, two calibration curves allowing quantitative measurements were obtained and compared on the basis of the SERS maps acquired on the samples loaded with several miRNA concentrations. The selectivity of the two-step assay was confirmed by the detection of target miR-222 mixed with different synthetic oligos, simulating the hybridization interference coming from similar sequences in real biological samples. Finally, that protocol was applied to the analysis of miR-222 in cellular extracts using an optofluidic multichamber biosensor, confirming the potentialities of SERS-based microfluidics for early-cancer diagnosis.

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16

Sharma, Kirti, Zoë Jäckel, Artur Schneider, Oliver Paul, Ilka Diester, and Patrick Ruther. "Multifunctional optrode for opsin delivery, optical stimulation, and electrophysiological recordings in freely moving rats." Journal of Neural Engineering 18, no. 6 (November 15, 2021): 066013. http://dx.doi.org/10.1088/1741-2552/ac3206.

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Abstract Objective. Optogenetics involves delivery of light-sensitive opsins to the target brain region, as well as introduction of optical and electrical devices to manipulate and record neural activity, respectively, from the targeted neural population. Combining these functionalities in a single implantable device is of great importance for a precise investigation of neural networks while minimizing tissue damage. Approach. We report on the development, characterization, and in vivo validation of a multifunctional optrode that combines a silicon-based neural probe with an integrated microfluidic channel, and an optical glass fiber in a compact assembly. The silicon probe comprises an 11-µm-wide fluidic channel and 32 recording electrodes (diameter 30 µm) on a tapered probe shank with a length, thickness, and maximum width of 7.5 mm, 50 µm, and 150 µm, respectively. The size and position of fluidic channels, electrodes, and optical fiber can be precisely tuned according to the in vivo application. Main results. With a total system weight of 0.97 g, our multifunctional optrode is suitable for chronic in vivo experiments requiring simultaneous drug delivery, optical stimulation, and neural recording. We demonstrate the utility of our device in optogenetics by injecting a viral vector carrying a ChR2-construct in the prefrontal cortex and subsequent photostimulation of the transduced neurons while recording neural activity from both the target and adjacent regions in a freely moving rat for up to 9 weeks post-implantation. Additionally, we demonstrate a pharmacological application of our device by injecting GABA antagonist bicuculline in an anesthetized rat brain and simultaneously recording the electrophysiological response. Significance. Our triple-modality device enables a single-step optogenetic surgery. In comparison to conventional multi-step surgeries, our approach achieves higher spatial specificity while minimizing tissue damage.

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17

Witkowska Nery, Emilia, Magdalena Kundys-Siedlecka, Yoshitaka Furuya, and Martin Jönsson-Niedziółka. "Pencil Lead as a Material for Microfluidic 3D-Electrode Assemblies." Sensors 18, no. 11 (November 19, 2018): 4037. http://dx.doi.org/10.3390/s18114037.

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We present an electrochemical, microfluidic system with a working electrode based on an ordered 3D array of pencil leads. The electrode array was integrated into a plexiglass/PDMS channel. We tested the setup using a simple redox probe and compared the results with computer simulations. As a proof of concept application of the device we showed that the setup can be used for determination of dopamine concentration in physiological pH and ultrasensitive, although only qualitative, detection of p-nitrophenol with a limit of detection below 1 nmol L−1. The observed limit of detection for p-nitrophenol is not only much lower than achieved with similar methods but also sufficient for evaluation of exposure to pesticides such as methyl parathion through urinalysis. This low cost setup can be fabricated without the need for clean room facilities and in the future, due to the ordered structure of the electrode could be used to better understand the process of electroanalysis and electrode functionalization. To the best of our knowledge it is the first application of pencil leads as 3D electrochemical sensor in a microfluidic channel.

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18

Wang, Anyan, Zhenhua Wu, Yuhang Huang, Hongbo Zhou, Lei Wu, Chunping Jia, Qiang Chen, and Jianlong Zhao. "A 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of Mycoplasma pneumoniae." Biosensors 11, no. 11 (October 29, 2021): 427. http://dx.doi.org/10.3390/bios11110427.

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Mycoplasma pneumonia (MP) is a common respiratory infection generally treated with macrolides, but resistance mutations against macrolides are often detected in mycoplasma pneumoniae in China. Rapid and accurate identification of mycoplasma pneumoniae and its mutant type is necessary for precise medication. This paper presents a 3D-printed microfluidic device to achieve this. By 3D printing, the stereoscopic structures such as microvalves, reservoirs, drainage tubes, and connectors were fabricated in one step. The device integrated commercial polymerase chain reaction (PCR) tubes as PCR chambers. The detection was a sample-to-answer procedure. First, the sample, a PCR mix, and mineral oil were respectively added to the reservoirs on the device. Next, the device automatically mixed the sample with the PCR mix and evenly dispensed the mixed solution and mineral oil into the PCR chambers, which were preloaded with the specified primers and probes. Subsequently, quantitative real-time PCR (qPCR) was carried out with the homemade instrument. Within 80 min, mycoplasma pneumoniae and its mutation type in the clinical samples were determined, which was verified by DNA sequencing. The easy-to-make and easy-to-use device provides a rapid and integrated detection approach for pathogens and antibiotic resistance mutations, which is urgently needed on the infection scene and in hospital emergency departments.

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Teixeira, Joana, Vicente Rocha, João Oliveira, Pedro A. S. Jorge, and Nuno A. Silva. "Towards real-time identification of trapped particles with UMAP-based classifiers." Journal of Physics: Conference Series 2407, no. 1 (December 1, 2022): 012043. http://dx.doi.org/10.1088/1742-6596/2407/1/012043.

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Abstract Optical trapping provides a way to isolate, manipulate, and probe a wide range of microscopic particles. Moreover, as particle dynamics are strongly affected by their shape and composition, optical tweezers can also be used to identify and classify particles, paving the way for multiple applications such as intelligent microfluidic devices for personalized medicine purposes, or integrated sensing for bioengineering. In this work, we explore the possibility of using properties of the forward scattered radiation of the optical trapping beam to analyze properties of the trapped specimen and deploy an autonomous classification algorithm. For this purpose, we process the signal in the Fourier domain and apply a dimensionality reduction technique using UMAP algorithms, before using the reduced number of features to feed standard machine learning algorithms such as K-nearest neighbors or random forests. Using a stratified 5-fold cross-validation procedure, our results show that the implemented classification strategy allows the identification of particle material with accuracies up to 80%, demonstrating the potential of using signal processing techniques to probe properties of optical trapped particles based on the forward scattered light. Furthermore, preliminary results of an autonomous implementation in a standard experimental optical tweezers setup show similar differentiation capabilities for real-time applications, thus opening some opportunities towards technological applications such as intelligent microfluidic devices and solutions for biochemical and biophysical sensing.

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Jang, Ling-Sheng, Hsin-Hung Li, Jen-Yu Jao, Ming-Kun Chen, and Chia-Feng Liu. "Design and fabrication of microfluidic devices integrated with an open‐ended MEMS probe for single‐cell impedance measurement." Microfluidics and Nanofluidics 8, no. 4 (July 29, 2009): 509–19. http://dx.doi.org/10.1007/s10404-009-0480-z.

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21

Tang, Dianping, Ruo Yuan, and Yaqin Chai. "Magnetic Control of an Electrochemical Microfluidic Device with an Arrayed Immunosensor for Simultaneous Multiple Immunoassays." Clinical Chemistry 53, no. 7 (July 1, 2007): 1323–29. http://dx.doi.org/10.1373/clinchem.2006.085126.

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Abstract Background: Methods based on magnetic bead probes have been developed for immunoassay, but most involve complicated labeling or stripping procedures and are unsuitable for routine use. Methods: We synthesized magnet core/shell NiFe2O4/SiO2 nanoparticles and fabricated an electrochemical magnetic controlled microfluidic device for the detection of 4 tumor markers. The immunoassay system consisted of 5 working electrodes and an Ag/AgCl reference electrode integrated on a glass substrate. Each working electrode contained a different antibody immobilized on the NiFe2O4/SiO2 nanoparticle surface and was capable of measuring a specific tumor marker using noncompetitive electrochemical immunoassay. Results: Under optimal conditions, the multiplex immunoassay enabled the simultaneous detection of 4 tumor markers. The sensor detection limit was <0.5 μg/L (or <0.5 kunits/L) for most analytes. Intra- and interassay imprecisions (CVs) were <4.5% and 8.7% for analyte concentrations >5 μg/L (or >5 kunits/L), respectively. No nonspecific adsorption was observed during a series of procedures to detect target proteins, and electrochemical cross-talk (CV) between neighboring sites was <10%. Conclusion: This immunoassay system offers promise for label-free, rapid, simple, cost-effective analysis of biological samples. Importantly, the chip-based immunosensor could be suitable for use in the mass production of miniaturized lab-on-a-chip devices and open new opportunities for protein diagnostics and biosecurity.

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Wang, Chih-Hung, Chia-Jung Chang, Jiunn-Jong Wu, and Gwo-Bin Lee. "An integrated microfluidic device utilizing vancomycin conjugated magnetic beads and nanogold-labeled specific nucleotide probes for rapid pathogen diagnosis." Nanomedicine: Nanotechnology, Biology and Medicine 10, no. 4 (May 2014): 809–18. http://dx.doi.org/10.1016/j.nano.2013.10.013.

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23

Perrault, C. M., M. A. Qasaimeh, T. Brastaviceanu, K. Anderson, Y. Kabakibo, and D. Juncker. "Integrated microfluidic probe station." Review of Scientific Instruments 81, no. 11 (November 2010): 115107. http://dx.doi.org/10.1063/1.3497302.

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24

Zhu, Liang, Xue Li Peh, Hong Miao Ji, Cheng Yong Teo, Han Hua Feng, and Wen-Tso Liu. "Cell loss in integrated microfluidic device." Biomedical Microdevices 9, no. 5 (May 31, 2007): 745–50. http://dx.doi.org/10.1007/s10544-007-9085-z.

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25

Li, Junji, Yuxiang Zhang, Zhan Qi, Jing Tu, and Zuhong Lu. "An Integrated Microfluidic Device for Droplet Manipulation." Journal of Nanoscience and Nanotechnology 16, no. 7 (July 1, 2016): 7164–69. http://dx.doi.org/10.1166/jnn.2016.11303.

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26

Zheng, Jun, James R. Webster, Carlos H. Mastrangelo, Victor M. Ugaz, Mark A. Burns, and David T. Burke. "Integrated plastic microfluidic device for ssDNA separation." Sensors and Actuators B: Chemical 125, no. 1 (July 2007): 343–51. http://dx.doi.org/10.1016/j.snb.2007.02.036.

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27

Ryu, Kee Suk, Xuefeng Wang, Kashan Shaikh, David Bullen, Edgar Goluch, Jun Zou, Chang Liu, and Chad A. Mirkin. "Integrated microfluidic linking chip for scanning probe nanolithography." Applied Physics Letters 85, no. 1 (July 5, 2004): 136–38. http://dx.doi.org/10.1063/1.1771453.

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Wu, Xiaosong, Jingyu Pan, Xinchao Zhu, Chenggang Hong, Anzhong Hu, Cancan Zhu, Yong Liu, Ke Yang, and Ling Zhu. "MS2 device: smartphone-facilitated mobile nucleic acid analysis on microfluidic device." Analyst 146, no. 12 (2021): 3823–33. http://dx.doi.org/10.1039/d1an00367d.

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Svoboda, Miloš, Zdeněk Slouka, Walter Schrott, and Dalimil Šnita. "Cation exchange membrane integrated into a microfluidic device." Microelectronic Engineering 86, no. 4-6 (April 2009): 1371–74. http://dx.doi.org/10.1016/j.mee.2009.01.019.

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Niibe, Kenta, and Hiroaki Onoe. "Stimuli-responsive hydrogel sensors integrated with microfluidic device." Proceedings of the Symposium on Micro-Nano Science and Technology 2017.8 (2017): PN—85. http://dx.doi.org/10.1299/jsmemnm.2017.8.pn-85.

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Binsley, Jacob L., Elizabeth L. Martin, Thomas O. Myers, Stefano Pagliara, and Feodor Y. Ogrin. "Elasto-Magnetic Pumps Integrated within Microfluidic Devices." Engineering Proceedings 4, no. 1 (April 16, 2021): 48. http://dx.doi.org/10.3390/micromachines2021-09590.

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Many lab-on-a-chip devices require a connection to an external pumping system in order to perform their function. While this is not problematic in typical laboratory environments, it is not always practical when applied to point-of-care testing, which is best utilized outside of the laboratory. Therefore, there has been a large amount of ongoing research into producing integrated microfluidic components capable of generating effective fluid flow from on-board the device. This research aims to introduce a system that can produce practical flow rates, and be easily fabricated and actuated using readily available techniques and materials. We show how an asymmetric elasto-magnetic system, inspired by Purcell’s three-link swimmer, can provide this solution through the generation of non-reciprocal motion in an enclosed environment. The device is fabricated monolithically within a microfluidic channel at the time of manufacture, and is actuated using a weak, oscillating magnetic field. The flow rate can be altered dynamically, and the direction of the resultant flow can be controlled by adjusting the frequency of the driving field. The device has been proven, experimentally and numerically, to operate effectively when applied to fluids with a range of viscosities. Such a device may be able to replace external pumping systems in portable applications.

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Pollard, Marcus, Rushabh Maugi, and Mark Platt. "Multi-resistive pulse sensor microfluidic device." Analyst 147, no. 7 (2022): 1417–24. http://dx.doi.org/10.1039/d2an00128d.

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A dual tuneable flow resistive pulse sensor which utilises additively manufactured parts. The sensor allows parts to be easily changed, washed and cleaned, its simplicity and versatility allow components from existing nanopore techniques to be integrated into a single device.

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Lee, Kyoung G., Kyun Joo Park, Seunghwan Seok, Sujeong Shin, Do Hyun Kim, Jung Youn Park, Yun Seok Heo, Seok Jae Lee, and Tae Jae Lee. "3D printed modules for integrated microfluidic devices." RSC Adv. 4, no. 62 (2014): 32876–80. http://dx.doi.org/10.1039/c4ra05072j.

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Cheon, Jeonghyeon, and Seunghyun Kim. "Fabrication and Demonstration of a 3D-printing/PDMS Integrated Microfluidic Device." Recent Progress in Materials 4, no. 1 (October 21, 2021): 1. http://dx.doi.org/10.21926/rpm.2201002.

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3D printing is an attractive method to fabricate microfluidic devices due to (1) its fast and simple process without specialized equipment and cleanroom environment, and (2) its capability to create complex 3D structures. Combined with Polydimethylsiloxane (PDMS), it can be used to develop various microfluidic devices taking advantage of both 3D printing and PDMS. In this paper, we investigated a Digital Light Processing (DLP) 3D printer to fabricate 3D printing/PDMS integrated microfluidic devices. We used it to fabricate both a master mold for the PDMS process and a substrate containing pneumatic ports and channels. The optimal design parameters to print a symmetrical microchannel structure were determined. We also measured the printing accuracy of taper structures as an example of its capability to fabricate complex structures. Then, we fabricated a microfluidic device by integrating a PDMS component with a 3D printed substrate. The microfluidic device operation was demonstrated using dye solutions. The fluidic control results clearly show the microfluidic device works as expected.

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Tonooka, Taishi. "Microfluidic Device with an Integrated Freeze-Dried Cell-Free Protein Synthesis System for Small-Volume Biosensing." Micromachines 12, no. 1 (December 29, 2020): 27. http://dx.doi.org/10.3390/mi12010027.

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Microfluidic devices enable the precise operation of liquid samples in small volumes. This motivates why microfluidic devices have been applied to point-of-care (PoC) liquid biopsy. Among PoC liquid biopsy studies, some report diagnostic reagents being freeze-dried in such microfluidic devices. This type of PoC microfluidic device has distinct advantages, such as simplicity of the procedures, compared with other PoC devices using liquid-type diagnostic reagents. Despite the attractive characteristic, only diagnostic reagents based on the cloned enzyme donor immunoassay (CEDIA) have been freeze-dried in the microfluidic device. However, development of the PoC device based on the CEDIA method is time-consuming and labor-intensive. Here, we employed a molecule-responsive protein synthesis system as the diagnostic reagent to be freeze-dried in the microfluidic device. Such molecule-responsive protein synthesis has been well investigated in the field of molecular biology. Therefore, using the accumulated information, PoC devices can be efficiently developed. Thus, we developed a microfluidic device with an integrated freeze-dried molecule-responsive protein synthesis system. Using the developed device, we detected two types of bio-functional molecules (i.e., bacterial quorum sensing molecules and mercury ions) by injecting 1 µL of sample solution containing these molecules. We showed that the developed device is applicable for small-volume biosensing.

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Watts, Benjamin R., Thomas Kowpak, Zhiyi Zhang, Chang-Qing Xu, Shiping Zhu, Xudong Cao, and Min Lin. "Fabrication and Performance of a Photonic-Microfluidic Integrated Device." Micromachines 3, no. 1 (February 15, 2012): 62–77. http://dx.doi.org/10.3390/mi3010062.

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Kipper, Sarit, Ludmila Frolov, Ortal Guy, Michal Pellach, Yair Glick, Asaf Malichi, Binyamin A. Knisbacher, et al. "Control and automation of multilayered integrated microfluidic device fabrication." Lab on a Chip 17, no. 3 (2017): 557–66. http://dx.doi.org/10.1039/c6lc01534d.

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Jang, Yun-Ho, Matthew J. Hancock, Sang Bok Kim, Šeila Selimović, Woo Young Sim, Hojae Bae, and Ali Khademhosseini. "An integrated microfluidic device for two-dimensional combinatorial dilution." Lab on a Chip 11, no. 19 (2011): 3277. http://dx.doi.org/10.1039/c1lc20449a.

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Kim, Tyson N., Kyle Campbell, Alex Groisman, David Kleinfeld, and Chris B. Schaffer. "Femtosecond laser-drilled capillary integrated into a microfluidic device." Applied Physics Letters 86, no. 20 (May 16, 2005): 201106. http://dx.doi.org/10.1063/1.1926423.

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Hoi, Siew-Kit, Zhi-Bin Hu, Yuanjun Yan, Chorng-Haur Sow, and Andrew A. Bettiol. "A microfluidic device with integrated optics for microparticle switching." Applied Physics Letters 97, no. 18 (November 2010): 183501. http://dx.doi.org/10.1063/1.3512902.

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LIU, R., S. MUNRO, T. NGUYEN, T. SIUDA, D. SUCIU, M. BIZAK, M. SLOTA, H. FUJI, D. DANLEY, and A. MCSHEA. "Integrated Microfluidic CustomArray Device for Bacterial Genotyping and Identification." Journal of the Association for Laboratory Automation 11, no. 6 (December 2006): 360–67. http://dx.doi.org/10.1016/j.jala.2006.07.004.

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Marchiarullo, Daniel J., Ji Y. Lim, Zalman Vaksman, Jerome P. Ferrance, Lakshmi Putcha, and James P. Landers. "Towards an integrated microfluidic device for spaceflight clinical diagnostics." Journal of Chromatography A 1200, no. 2 (July 2008): 198–203. http://dx.doi.org/10.1016/j.chroma.2008.05.031.

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Zhao, Wang, Li Zhang, Wenwen Jing, Sixiu Liu, Hiroshi Tachibana, Xunjia Cheng, and Guodong Sui. "An integrated microfluidic device for rapid serodiagnosis of amebiasis." Biomicrofluidics 7, no. 1 (January 2013): 011101. http://dx.doi.org/10.1063/1.4793222.

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Huang, Mo Chao, Hongye Ye, Yoke Kong Kuan, Mo-Huang Li, and Jackie Y. Ying. "Integrated two-step gene synthesis in a microfluidic device." Lab Chip 9, no. 2 (2009): 276–85. http://dx.doi.org/10.1039/b807688j.

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McMillan, Alexander H., Emma K. Thomée, Alessandra Dellaquila, Hussam Nassman, Tatiana Segura, and Sasha Cai Lesher-Pérez. "Rapid Fabrication of Membrane-Integrated Thermoplastic Elastomer Microfluidic Devices." Micromachines 11, no. 8 (July 28, 2020): 731. http://dx.doi.org/10.3390/mi11080731.

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Leveraging the advantageous material properties of recently developed soft thermoplastic elastomer materials, this work presents the facile and rapid fabrication of composite membrane-integrated microfluidic devices consisting of FlexdymTM polymer and commercially available porous polycarbonate membranes. The three-layer devices can be fabricated in under 2.5 h, consisting of a 2-min hot embossing cycle, conformal contact between device layers and a low-temperature baking step. The strength of the FlexdymTM-polycarbonate seal was characterized using a specialized microfluidic delamination device and an automated pressure controller configuration, offering a standardized and high-throughput method of microfluidic burst testing. Given a minimum bonding distance of 200 μm, the materials showed bonding that reliably withstood pressures of 500 mbar and above, which is sufficient for most microfluidic cell culture applications. Bonding was also stable when subjected to long term pressurization (10 h) and repeated use (10,000 pressure cycles). Cell culture trials confirmed good cell adhesion and sustained culture of human dermal fibroblasts on a polycarbonate membrane inside the device channels over the course of one week. In comparison to existing porous membrane-based microfluidic platforms of this configuration, most often made of polydimethylsiloxane (PDMS), these devices offer a streamlined fabrication methodology with materials having favourable properties for cell culture applications and the potential for implementation in barrier model organ-on-chips.

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Kim, Jaehoon, Junghyo Yoon, Jae-Yeong Byun, Hyunho Kim, Sewoon Han, Junghyun Kim, Jeong Hoon Lee, Han-Sang Jo, and Seok Chung. "Nano-Interstice Driven Powerless Blood Plasma Extraction in a Membrane Filter Integrated Microfluidic Device." Sensors 21, no. 4 (February 15, 2021): 1366. http://dx.doi.org/10.3390/s21041366.

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Blood plasma is a source of biomarkers in blood and a simple, fast, and easy extraction method is highly required for point-of-care testing (POCT) applications. This paper proposes a membrane filter integrated microfluidic device to extract blood plasma from whole blood, without any external instrumentation. A commercially available membrane filter was integrated with a newly designed dual-cover microfluidic device to avoid leakage of the extracted plasma and remaining blood cells. Nano-interstices installed on both sides of the microfluidic channels actively draw the extracted plasma from the membrane. The developed device successfully supplied 20 μL of extracted plasma with a high extraction yield (~45%) in 16 min.

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Li, Da Lei, Xue Fei Lv, and Yu Lin Deng. "A Microfluidic Device for Sample Pretreatment by Laminar Flow Extraction." Applied Mechanics and Materials 511-512 (February 2014): 8–11. http://dx.doi.org/10.4028/www.scientific.net/amm.511-512.8.

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Microfluidic chip is the most active field and frontier of μTAS. In comparison to other aspects of researches on microfluidic chips, the work on sample pretreatment units are in the preliminary stage. In this study, a microfluidic device for extraction was devised and fabricated. The extraction efficiency of the microfluidic device was investigated by two phase fluid 0.1% Rhodamine-B aqueous solution and Hexyl alcohol. The results demonstrated that the microfluidic chip worked well in the first two days and might be integrated in a complex chip as a potential tool for sample pretreatment.

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Huang, Kuo-Wei, Sabbir Sattar, Jiang F. Zhong, Cheng-Hsu Chou, Hsiung-Kuang Tsai, and Pei-Yu Chiou. "Electrodes for Microfluidic Integrated Optoelectronic Tweezers." Advances in OptoElectronics 2011 (October 11, 2011): 1–10. http://dx.doi.org/10.1155/2011/375451.

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We report on two types of electrodes that enable the integration of optoelectronic tweezers (OETs) with multilayer poly(dimethylsilane)- (PDMS-) based microfluidic devices. Both types of electrodes, Au-mesh and single-walled carbon nanotube- (SWNT-) embedded PDMS thin film, are optically transparent, electrically conductive, and can be mechanically deformed and provide interfaces to form strong covalent bonding between an OET device and PDMS through standard oxygen plasma treatment. Au-mesh electrodes provide high electrical conductivity and high transparency but are lack of flexibility and allow only small deformation. On the other hand, SWNT-embedded PDMS thin film electrodes provide not only electrical conductivity but also optical transparency and can undergo large mechanical deformation repeatedly without failure. This enables, for the first time, microfluidic integrated OET with on-chip valve and pump functions, which is a critical step for OET-based platforms to conduct more complex and multistep biological and biochemical analyses.

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Sun, Yawei, Yaopeng Zhang, Jingjing Liu, and Fuqiang Nie. "Integrated microfluidic device for the spherical hydrogel pH sensor fabrication." RSC Advances 6, no. 14 (2016): 11204–10. http://dx.doi.org/10.1039/c5ra25893f.

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Chen, Jhih-Siang, Pin-Fan Chen, Hana Tzu-Han Lin, and Nien-Tsu Huang. "A Localized surface plasmon resonance (LSPR) sensor integrated automated microfluidic system for multiplex inflammatory biomarker detection." Analyst 145, no. 23 (2020): 7654–61. http://dx.doi.org/10.1039/d0an01201g.

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