Dissertations / Theses on the topic 'Microfluidic Probe Integrated Device'
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Shaw, Kirsty Jane. "Integrated DNA extraction and amplification on a microfluidic device." Thesis, University of Hull, 2009. http://hydra.hull.ac.uk/resources/hull:2414.
Full textRivas, Cardona Juan Alberto. "Development of a microfluidic device for patterning multiple species by scanning probe lithography." [College Station, Tex. : Texas A&M University, 2006. http://hdl.handle.net/1969.1/ETD-TAMU-1823.
Full textDhopeshwarkar, Rahul Rajesh. "Electrokinetic concentration enrichment within a microfluidic device integrated with a hydrogel microplug." [College Station, Tex. : Texas A&M University, 2007. http://hdl.handle.net/1969.1/ETD-TAMU-1442.
Full textSánchez, Daniela [Verfasser], and A. [Akademischer Betreuer] Guber. "Automated Integrated Microfluidic Device for DNA Cloning / Daniela Sánchez ; Betreuer: A. Guber." Karlsruhe : KIT-Bibliothek, 2019. http://d-nb.info/1184990085/34.
Full textHui, Lawrence Kwan Yeung. "Integrated microfluidic device for single-cell high throughput screening in dynamic gene expression analysis." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2008. http://wwwlib.umi.com/cr/ucsd/fullcit?p1457283.
Full textTitle from first page of PDF file (viewed November 13, 2008). Available via ProQuest Digital Dissertations. Includes bibliographical references (p. 58-60).
Epshteyn, Alla. "Design and Fabrication of a Membrane Integrated Microfluidic Cell Culture Device Suitable for High-Resolution Imaging." Scholar Commons, 2010. http://scholarcommons.usf.edu/etd/3517.
Full textDimopoulos, Georges. "Etude, caractérisation et modélisation des micromagnétodiodes à grille en silicium sur saphir." Grenoble INPG, 1989. http://www.theses.fr/1989INPG0015.
Full textAlexander, Stewart Parks. "An integrated microoptical microfluidic device for agglutination detection and blood typing." 2007. http://www.lib.ncsu.edu/theses/available/etd-01082007-035319/unrestricted/etd.pdf.
Full textYa-ChunChuang and 莊雅鈞. "Affinity Biosensor of HbA1c Integrated with Microfluidic Device Based on Impedance Measurement." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/26650499095019854030.
Full textTung, Chen-Kuo, and 董震國. "In Vitro Lobule Mimic Microfluidic Device Integrated with Dielectrophoresis Patterning and Gelatin Methacrylate Hydrogels." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/pyavcw.
Full text國立清華大學
動力機械工程學系
102
This thesis reports a lobule-mimicking chip, which was dedicated to reconstruct lobule tissue in vitro. In order to maintain the pattern of heterogeneous cells, the biocompatible material, Gelatin methacrylate (GelMA) hydrogels, was used. GelMA is a photocrosslinkable material which could be photocrosslinked under UV light. The chip design was divided into two parts: the first part includes the GelMA concentration generator, and the second part is composed of the cell patterning and the GelMA covering chamber. The GelMA concentration generator could generate three kinds of GelMA concentration: 5%, 10%, and 15%. In the cell culture chamber, 17 sets of hexagonal electrodes were located inside of it. Each set of hexagonal electrodes was used to attract and pattern 3T3 cells and C3A cells. After two kinds of cells were patterned and attracted to the defined location, different concentration of GelMA was loaded into the cell culture chamber. In order to understand the influence between different concentration of GelMA and cells, the tests of viability and urea had been performed. The viability of cells under 5% of GelMA can maintain 95% after72-hrs culture. By increasing the concentration of GelMA, the cell viability would decrease down to 78%. The urea assay showed that cells covered by GelMA can still maintain their activity. The results also showed that patterned cells covered by GelMA modules can maintain their pattern after 3-days culture. Compared with the patterned cells without covering GelMA modules, 3T3 cells grow faster than C3A cells and migrate above C3A cells.
Lee, Chen-Yan, and 李承諺. "Microfluidic Particles / Cells Sorter Integrated with Concentration Friction Feeding Device for Biochemical Analysis Applications." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/42569358528732372732.
Full text國立中山大學
機械與機電工程學系研究所
94
This study proposes a navel method for continuously particle sorting utilizing cascade squeeze jumping effect under microfluidic configuration. Microparticles with different sizes can be successfully separated at different stages of squeezing sheath flow. The method is based on that particles can not flow stably within a flow stream with the smaller stream width than their sizes. Big particles will jump from their original flow stream into the wider neighboring sheath flow. In this study, we have successfully designed and fabricated two kinds of particles/cells sorters using MEMS (Micro-electro-mechanical Systems) technology. The proposed microchip device includes a multi-stage sheath flow particles/cells sorter and an improved design of a cascade squeezed flow scheme. In the study, theoretical formulations, computer simulations and experimental operations are used to analyze the flow field in the microchip and evaluate the sorting performance of the devices. Results show the good sorting performance with cell recovery rate of 87.7% and yield rate of 94.1% can be obtained using the proposed micro particles/cells sorter. Furthermore, it is also important to continiously prepare reagents for in-column bio-chemical reactions. Therefore, this study presents a sheath-flow based microfluidic device for concentration fraction delivery of liquid samples. The simple and novel structure proposed in this study is able to prepare reagent with different concentration and is also easy to be integrated with other multifunctional microfluidic device. In order to demonstrate the feasibility and performance of the proposed concentration fraction delivery device, this study designs an integrated microchip device for in-line preparation of lysin reagent for cell lysis and an integrated T-form microfluidic mixer for demonstration of RBC lysis in the same microchip. Reagents for cell lysis are firstly prepared by the concentration faction delivery part of the chip. The prepared reagent is mixed with RBC sample downstream in the reaction channel using the T-form mixer. Results show a high RBC lysing rate of upto 100% in 10 mm downstream the T-junction can be achieved utilizing the proposed chip. In this study, we have successfully demonstrated three kinds of microfluidic device including a micro particles/cells sorter, a concentration fraction delivery device and a cell lysis reactor. Numerical analysis and experimental investigation confirm the proposed concepts and performance of the microfluidic devices. The contributions of the study are highly potential for developing a low-cost bioreactor system in the
Lien, Kang-Yi, and 連剛逸. "Miniature Microfluidic System Integrated with a Sample Pretreatment Device for Rapid Nucleic Acid Amplification." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/84844620753199418940.
Full text國立成功大學
奈米科技暨微系統工程研究所
95
Recently, purification and enrichment of bio-samples is crucial for the analysis of biosamples with an ultra-low concentration that the performance of the detection system can be efficiently increased. Apart from that, clinical samples usually contain biological medium that would normally inhibit the subsequent ribonucleic acid (RNA)/deoxyribonucleic acid (DNA) amplification process. Moreover, there still need several tedious purification and washing steps and labor-intensive processes to complete the sample preparation. In addition, a number of large-scale equipments are usually needed in the complex pretreatment procedures and the bio-samples may also be wasted and cause some contaminations during manual operations. To improve this, the concept of MEMS (micro-electro-mechanical-system)-based miniaturization is applied in the magnet beard-based sample purification and enrichment process. It is generally believed that the miniaturized bio-devices typically bring the advantages of shorter diagnosis times, less sample and reagent consumption, improved resolution, higher sensitivity, and lower cost. By the incorporation of the microfluidic system, the miniature bio-devices provide the powerful techniques to transport or mix the fluids in the system and make the biological diagnosis a quick and automatic process. Nevertheless, the integrated systems still need other detection chambers to perform biological diagnosis. And the on-chip microfluidic modules still need several sets of unidirectional microfluidic structures to complete the whole process. As a result, several electromagnetic valves (EMVs) are usually required to deliver the fluid in the microchannels and the manual operations are normally required since the lack of function of sample pretreatment in the micro system. Consequently, there is a need for a sample pretreatment process by utilizing a microfluidic system to automate the nucleic acid amplification process with less human intervention and also to improve sensitivity and selectivity. The study therefore proposes three new miniature reverse transcription polymerase chain reaction (RT-PCR) systems that integrate with bio-sample pretreatment devices using superparamagnetic beads into a single chip platform. In the first system, three modules were integrated including a microfluidic nodule consists of three sets of novel pneumatic micropumps, a bead collection module with Au wires and a micro temperature control module for the polymerase chain reaction (PCR) process. For the second system proposed, the microfluidic chip integrated two functional devices for bio-samples purification and enrichment including pumping, mixing and separation by utilizing a rotary microfluidic module and a bead-collection module consists of 2-dimension/3-dimension (2-D/3-D) microcoils. The original rotary microfluidic module can provide a rapid flow pumping rate and a high mixing efficiency and can pretreat the bio-samples in a short period of time. The third microsystem uses the novel microfluidic system including a two-way serpentine-shape (s-shape) micropump requiring only one EMV to transport and to mix the bio-samples in the microchannels. In addition, new bio-separators are developed either to perform the separation of magnetic beads or to control the temperature field for the subsequent RT-PCR process. The rapid heating/cooling rate of the micro-heating chambers can significantly shorten the pre-treatment and diagnosis processes. As a whole, the developed system may provide a powerful platform integrating the functions of sample pretreatment and fast disease diagnosis.
Chu, Sheng-Han, and 朱聖瀚. "A Microfluidic Device Integrated Superresolution Microscopy forIn-situ Fixation and Mechanosensation of Primary Cilia." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/sex4dj.
Full text國立臺灣大學
生醫電子與資訊學研究所
107
Primary cilium is an organelle found in most mammalian cells. It is known that primary cilium can sense fluid flow, molecules and light in order to transfer these stimulations into cells. Mechanosensation which senses the mechanical stimulation and transfers to cells is the main function of primary cilia and associated with various syndrome. Due to the size of primary cilium is around few micrometers, conventional research methods cannot provide precise stimulation. At the same time, diffraction limit also makes direct observation of primary cilia more difficult. Presently, primary cilium length, intercellular calcium concentration and gene expression are commonly used to observe the function of primary cilium. Microfluidics is a method which control fluid under micrometer scale. It has the advantages of small volume, low cost and low sample demand compare to conventional methods. The ability of applying flow stimulation and switching fluid accurately makes microfluidic the most suitable tools to study primary cilia. Under the development of superresolution microscopy, protein composition, movement and numbers can be observed inside the primary cilium. This powerful tool help scientists study more detailed about mechanosensation of primary cilium. A device which can provide proper stimulation, real-time cilia bending and ability to do immunostaining is needed. We develop a multiple flow velocity and resealable microfluidic system. By applying precise flow velocity, it is capable to observe primary cilia change in live cell, do immunostaining of the desired protein after stimulation and analyze the result using epifluorescence or superresolution microscopy. In the experiments, we optimized the protocol to promote ciliogenesis, observed the bending in both live and immunostained images under the flow stimulation. Last but not least, by superresolution microscopy, we found the protein distribution inside primary cilia has huge differences before and after flow stimulation. We believe that the result is highly correlated to the signal transduction of primary cilia. In future work, we plan to do more relative experiments can be done by using our system. Also, the system has the potential to integrate with protein mass spectrometry to do high throughput protein analysis for primary cilia specific proteins.
Saaem, Ishtiaq. "Enabling Technologies for Synthetic Biology: Gene Synthesis and Error-Correction from a Microarray-Microfluidic Integrated Device." Diss., 2011. http://hdl.handle.net/10161/5719.
Full textPromising applications in the design of various biological systems hold critical implications as heralded in the rising field of synthetic biology. But, to achieve these goals, the ability to synthesize in situ DNA constructs of any size or sequence rapidly, accurately and economically is crucial. Today, the process of DNA oligonucleotide synthesis has been automated but the overall development of gene and genome synthesis technology has far lagged behind that of gene and genome sequencing. This has meant that numerous ideas go unfulfilled due to scale, cost and impediments in the quality of DNA due to synthesis errors.
This thesis presents the development of a multi-tool ensemble platform targeted at gene synthesis. An inkjet oligonucleotide synthesizer is constructed to synthesize DNA microarrays onto silica functionalized cylic olefin copolymer substrates. The arrays are married to microfluidic wells that provide a chamber to for enzymatic amplification and assembly of the DNA from the microarrays into a larger construct. Harvested product is then amplified off-chip and error corrected using a mismatch endonuclease-based reaction. This platform has the potential to be particularly low-cost since it employs standard phosphoramidite reagents and parts that are cheaper than optical and electrochemical systems. Genes sized 160 bp to 993 bp were successfully harvested and, after error correction, achieved up to 94% of intended functionality.
Dissertation
Kang, Luo, and 羅康. "An integrated array-based emulsion droplet microfluidic device for digital loop-mediated isothermal amplification (LAMP) analysis." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/99195092128832100956.
Full text國立清華大學
動力機械工程學系
104
Nucleic acid technology (NAT) based detection is a popular technique to detect nucleic acid molecules such as deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), which can be used for early diagnosis of cancer, confirmation of genetic disease, and many others. Usually, the copy number of the nucleic acid molecules in a sample is too less to be detected. As a result, the first step of nucleic acid detection is to amplify the nucleic acid molecules to a detectable level. Polymerase chain reaction (PCR) is one of the most commonly used methods for DNA or RNA amplification, in which nucleic acid samples go through thermal cycling between two or three different operating temperatures. The thermal cycling requires accurate and robust control of the operating temperature. To simplify the complicated processes of thermal cycling, isothermal amplification methods have been developed. Isothermal amplification reactions such as loop-mediated amplification (LAMP) can be implemented at a fixed temperature with no need for thermal cycling, which reduces the complexity of the temperature control module and consumes less energy, making them more suitable for microfluidics-based applications. Moreover, when compared with PCR, LAMP is more sensitive and specific. Furthermore, it takes less than one hour to finish the amplification process. These advantages make LAMP become a promising method for NAT based detection. In biomedical applications, it is often of great significance to quantify the accurate nucleic acid molecule copies in original samples. The original DNA copies can be measured by real-time PCR. However, the quantification relays on a reference or a standard curve. Besides, this bulk reaction fails to distinguish subtle difference of the target copy number. In an effort to overcome the disadvantages, digital DNA amplification has been developed. In this study, we reported a new method to implement array-based digital LAMP analysis, in which we integrated the emulsion droplet microfluidic device and hydrodynamic trapping techniques to form a droplet array which could be further applied digital LAMP assay. By generating monodisperse water-in-oil droplets which contain LAMP reaction mixture, the bulk LAMP reaction mixture could be partitioned into many separate compartments. Each droplet could function as a reaction chamber. Hydrodynamic trapping technique was further used for immobilizing the droplets to form a droplet array, which is good for later analysis of the result. In this work, we used our integrated microfluidic chip to demonstrate digital LAMP assay. The microfluidic chip could be able to generate uniform droplet with a size variation less than 3% and the droplet could be hydrodynamically immobilized to form a droplet array. Besides, the LAMP assay could be successfully implemented in water-in-oil droplet.
Chen, Pei-Zhen, and 陳姵蓁. "Development of A Microfluidic Device Integrated with A LC-MS/MS System for Detecting Psychoactive Substance." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/5tx286.
Full text國立臺灣科技大學
機械工程系
107
How to rapidly analyze the drug compound is an emerging demand and this study intends to integrate a microfluidic chip with a LC-MS/MS system to address this issue. Since the operation pressure of compound separation in a microchannel is relatively high and how to completely seal the separation particle inside the microchannel is quite difficult, chip manufacturing is quite challenging. Two approaches were used, (1) ethanol solution and wall structure with UV glue were used to bond the chip with securing the particles inside the microchannel; (2) C-type fixture was used to increase the bonding strength. Initial experiment result showed that this chip with filled particles can be potentially used as a commercial separation column for analyzing the drug compound with connecting to a LC-MS/MS system.
Lin, Jiun-Rue, and 林俊叡. "A Microfluidic Device Integrated with Electrochemical Impedance Spectroscopy Sensor for Whole Blood Plasma Separation and Hemoglobin Detection." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/10437927572960675313.
Full text國立臺灣大學
生醫電子與資訊學研究所
103
The paper presents a whole blood processing microfluidic system, consists of a PMMA microfluidic chip and an Electrochemical Impedance Spectroscopy (EIS) sensor. In this system, we used an engraving machine to fabricate a PMMA microfluidic chip. The microfluidic chip consists of two specific microstructural designs to perform efficient whole blood process. The first structure design is a series of curved microchannel to achieve blood plasma mixing, purification, dilution. The second structure design is a series of microtrench to achieve blood cell sedimentation. Finally, we used an EIS sensor to measure Hemoglobin from the blood sample after PMMA microfluidics process to prove the concept of this microfluidic system. Based on preliminary results in this thesis, we believe the system can eventually move toward to a simplified portable device for point-of-care applications.
Yu-CheHsieh and 謝侑哲. "Transportation and toxicity testing of zebrafish larvae through a noninvasive light driven technique integrated with a microfluidic device." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/3764sh.
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