Dissertations / Theses on the topic 'Microfluidic optical chip'
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Heinze, Brian Carl. "Lab-on-a-Chip Optical Immunosensor for Pathogen Detection." Diss., The University of Arizona, 2010. http://hdl.handle.net/10150/196023.
Full textMarchington, Robert F. "Applications of microfluidic chips in optical manipulation & photoporation." Thesis, University of St Andrews, 2010. http://hdl.handle.net/10023/1633.
Full textLucas, Lonnie J. "Detection of Light Scattering for Lab-On-A-Chip Immunoassays Using Optical Fibers." Diss., The University of Arizona, 2007. http://hdl.handle.net/10150/193897.
Full textBERRETTONI, CHIARA. "Design, implementation and characterization of an optoelectronic platform for the detection of immunosuppressants in transplanted patients by means of a microfluidic optical chip." Doctoral thesis, Università di Siena, 2017. http://hdl.handle.net/11365/1007099.
Full textKaylor, Sean C. "Development of a Low Cost Handheld Microfluidic Phosphate Colorimeter for Water Quality Analysis." DigitalCommons@CalPoly, 2009. https://digitalcommons.calpoly.edu/theses/147.
Full textHe, Yingning. "Lateral porous silicon membranes for planar microfluidic applications." Thesis, Toulouse 3, 2016. http://www.theses.fr/2016TOU30255/document.
Full textLab on a chip devices aim at integrating functions routinely used in medical laboratories into miniaturized chips to target health care applications with a promising impact foreseen in point-of-care testing. Porous membranes are of great interest for on-chip sample preparation and analysis since they enable size- and charge-based molecule separation, but also molecule pre-concentration by ion concentration polarization. Out of the various materials available to constitute porous membranes, porous silicon offers many advantages, such as tunable pore properties, large porosity, convenient surface chemistry and unique optical properties. Porous silicon membranes are usually integrated into fluidic chips by sandwiching fabricated membranes between two layers bearing inlet and outlet microchannels, resulting in three-dimensional fluidic networks that lack the simplicity of operation and direct observation accessibility of planar microfluidic devices. To tackle this constraint, we have developed two methods for the fabrication of lateral porous silicon membranes and their monolithic integration into planar microfluidics. The first method is based on the use of locally patterned electrodes to guide pore formation horizontally within the membrane in combination with silicon-on-insulator (SOI) substrates to spatially localize the porous silicon within the channel depth. The second method relies on the fact that the formation of porous silicon by anodization is highly dependent on the dopant type and concentration. While we still use electrodes patterned on the membrane sidewalls to inject current for anodization, the doping via implantation enables to confine the membrane analogously to but instead of the SOI buried oxide box. Membranes with lateral pores were successfully fabricated by these two methods and their functionality was demonstrated by conducting filtering experiments. In addition to sample filtration, we have achieved electrokinetic pre-concentration and interferometric sensing using the fabricated membranes. The ion selectivity of the microporous membrane enables to carry out sample pre-concentration by ion concentration polarization with concentration factors that can reach more than 103 in 10 min by applying less than 9 V across the membrane[TL1]. These results are comparable to what has already been reported in the literature using e.g. nanochannels with much lower power consumption. Finally, we were able to detect a change of the porous silicon refractive index through the shift of interference spectrum upon loading different liquids into the membrane. The work presented in this dissertation constitutes the first step in demonstrating the interest of porous silicon for all-in-one sample preparation and biosensing into planar lab on a chip
Schembri, Florinda. "Experimental study for the control of two-phase microfluidic flows." Thesis, Universita' degli Studi di Catania, 2011. http://hdl.handle.net/10761/366.
Full textHarazim, Stefan M. "Rolled-up microtubes as components for Lab-on-a-Chip devices." Doctoral thesis, Universitätsbibliothek Chemnitz, 2012. http://nbn-resolving.de/urn:nbn:de:bsz:ch1-qucosa-100312.
Full textDie auf verspannten Dünnschichten basierende „rolled-up nanotechnologie“ ist eine leistungsfähige Methode um dreidimensionale hohle Strukturen (Mikroröhrchen) aus nahezu jeder Art von Material auf einer großen Vielfalt von Substraten herzustellen. Ausgehend von der Möglichkeit der Skalierung des Röhrchendurchmessers und der Modifikation der Funktionalität des Röhrchens durch Einsatz verschiedener Materialien und Oberflächenfunktionalisierungen kann eine große Anzahl an verschiedenen Anwendungen ermöglicht werden. Eine Anwendung behandelt unter anderem on-chip Studien einzelner Zellen wobei die Mikroröhrchen, an die Größe der Zelle angepasste, Reaktionscontainer darstellen. Eine weitere Modifikation der Funktionalität der Mikroröhrchen kann durch das Aufbringen einer katalytischen Schicht realisiert werden, wodurch das Mikroröhrchen zu einem selbstangetriebenen katalytischen Mikro-Motor wird. Hauptziel dieser Arbeit ist es Mikrometer große optisch aktive Glasröhrchen herzustellen, diese mikrofluidisch zu kontaktieren und als Sensoren in Lab-on-a-Chip Systeme zu integrieren. Die integrierten Glasröhrchen arbeiten als optofluidische Ringresonatoren, welche die Veränderungen des Brechungsindex von Fluiden im inneren des Röhrchens durch Änderungen im Evaneszenzfeld detektieren können. Die Funktionsfähigkeit eines Demonstrators wird mit verschiedenen Flüssigkeiten gezeigt, dabei kommt ein Fotolumineszenz Spektrometer zum Anregen des Evaneszenzfeldes und Auslesen des Signals zum Einsatz. Die entwickelte Integrationsmethode ist eine Basis für ein kostengünstiges, zuverlässiges und reproduzierbares Herstellungsverfahren von optofluidischen Mikrochips basierend auf optisch aktiven Mikroröhrchen
Fu, Yi. "Conception, fabrication et expérimentation de systèmes microfluidiques de CULTU." Thesis, Paris Est, 2014. http://www.theses.fr/2014PEST1165/document.
Full textIn this PhD project, two in vitro cell culture devices were developed via microfabrication technologies, which provided entirely new levels of controls over the cell culture microenvironment. The applications of the developed devices in cancer and neurobiology researches were demonstrated, specifically for the fundamental study of cancer metastasis and neural axonal pathfinding. The microfluidic transmigration chip used microchannel structures to mimic the tissue capillaries along the path of cancer cell metastasis. The transparent optical qualities of the device allowed good observation of the deformation and migration of cells in the artificial capillaries. Results showed that deformation of the stiff cell nucleus were the most time-consuming steps during the transmigration process. The physical restrictions not only changed the morphology of the cells, but also significantly affect their migration profile. Further studies on the molecular contents and biological properties of the transmigrated cells showed that blocking the histone modifications by specific drug can inhibit the transmigration of cancer cells in the microchannel, which might have implications on cancer prevention and treatment. The microfluidic chip can also be used to evaluate cell deformability, which is a potential prognostic marker for cancer diagnosis. The neural culture chip integrated microfluidic cell culture and protein patterning techniques. The somas and axons of neurons cultured in the device can be polarized into different fluidically isolated environments for long period, and the extension of the axons can be guided by proteins immobilized on the glass substrate into specific patterns. The oriented axon growth can be further modulated by localized drug treatment. Studies on the underlying molecular mechanism revealed that these processes were closely associated with the proteins synthesized locally in the tips of growing axons
Shen, Li. "PORTABLE MULTIPLEXED OPTICAL DETECTION FOR POINT-OF-CARE." University of Cincinnati / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1367943692.
Full textScullion, Mark Gerard. "Slotted photonic crystal biosensors." Thesis, University of St Andrews, 2013. http://hdl.handle.net/10023/3405.
Full textMelin, Jonas. "Single-Molecule Detection and Optical Scanning in Miniaturized Formats." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7268.
Full textNovák, Pavel. "Vývoj cell-sorter systému s využitím optické pinzety a mikrofluidních čipů." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2011. http://www.nusl.cz/ntk/nusl-219024.
Full textWright, Jr Joel Greig. "An Integrated Model of Optofluidic Biosensor Function and Performance." BYU ScholarsArchive, 2021. https://scholarsarchive.byu.edu/etd/9267.
Full textSchimpf, Armin. "Réalisation d'un capteur intégré optique et microfluidique pour la mesure de concentration par effet photothermique." Phd thesis, Université de Grenoble, 2011. http://tel.archives-ouvertes.fr/tel-00824619.
Full textIazzolino, Antonio. "Engineering three-dimensional extended arrays of densely packed nano particles for optical metamaterials using microfluidIque evaporation." Phd thesis, Université Sciences et Technologies - Bordeaux I, 2013. http://tel.archives-ouvertes.fr/tel-01059235.
Full textWall, Thomas Allen. "Improved Single Molecule Detection Platform Using a Buried ARROW Design." BYU ScholarsArchive, 2017. https://scholarsarchive.byu.edu/etd/6535.
Full textHamilton, Erik Scott. "Three-Dimensional Hydrodynamic Focusing for Integrated Optofluidic Detection Enhancement." BYU ScholarsArchive, 2020. https://scholarsarchive.byu.edu/etd/8436.
Full textStott, Matthew Alan. "Multiplexed Optofluidics for Single-Molecule Analysis." BYU ScholarsArchive, 2018. https://scholarsarchive.byu.edu/etd/6740.
Full textLu, Wei-Chen, and 呂韋辰. "Development of a Microfluidic Chip for On-chip Optical Trapping." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/98175916906078524281.
Full text國立臺灣大學
光電工程學研究所
101
In the past decades, miniaturization has been the driving force for the development of technology. For the medical or biological research, traditionally they have to perform with bulky instruments and have to wait a long time to analyze the results. The research on lab-on-chip devices may lead to portable medical inspection devices. A lab-on-chip device is a versatile chip that integrates different kinds of functionalities into a small area ranging from millimeters to a few centimeters in size. The development of a lab-on-chip device can not only shrinks the experiment area to a small size but also enable a fast and reliable analysis. Recently, the research on single cells analysis is thriving. For this purpose, it is important to distinguish and to sort the cells based on their physical or chemical features. We want to develop a setup that is operating in a miniaturized area and that is able to hold a sample for a certain time so that we can gather its information. The technique of counter propagating dual fiber optical trap is appropriate to our demands, because the divergency of the optical fibers makes them possible to hold a larger sample in an optical trap. Besides, the optical fibers are flexible so that they can be easily integrated. To quantify the optical trapping performance on a chip, we have to establish a model to calculate the forces exerted by a light beam when it interacts with matter. The model is based on a ray tracing approach with the use of a non-sequential ray tracing software. The non-sequential ray tracing method allows for the considerations of any order of the interactions due to the “child rays” caused by reflection, refraction, etc at the interface between the light propagating medium and the trapped object. The model provides a powerful tool that can be used to design and optimize a microfluidic chip. The optical trapping will be operated in a microfluidic environment. Therefore, the trapping forces in the direction of the flow should be higher to resist the forces induced by the flow. The basic design of the fiber trapping on chip can be further improved by implementing microlenses in the chip. The design of the microfluidic chip is limited by the boundary conditions of the fabrication technique. It limits the minimum diameter of the microfluidic channel and the distance between the fiber facet and the trapped position. By considering the limitations and with the aid of the ray tracing model, the radius of curvature and the height of the lenses can be optimized towards the maximum transverse trapping force. The improvement results from the use of the microlenses can be shown by comparing the modeling results of the two optical trapping schemes. In order to show the validity of the ray tracing model, the proof of concept optical setup is under construction. The motions of the trapped object in its equilibrium position should be recorded and analyzed for the quantification of the trapping forces exerted by the laser beam. The novelty of our design, to our knowledge, lies in the use of the integrated microlenses to enhance the performance of an dual fiber optical trap in a microfluidic chip.
Luan, Lin. "Chip Scale Integrated Optical Sensing Systems with Digital Microfluidic Systems." Diss., 2010. http://hdl.handle.net/10161/3020.
Full textData acquisition and diagnostics for chemical and biological analytes are critical to medicine, security, and the environment. Miniaturized and portable sensing systems are especially important for medical and environmental diagnostics and monitoring applications. Chip scale integrated planar photonic sensing systems that can combine optical, electrical and fluidic functions are especially attractive to address sensing applications, because of their high sensitivity, compactness, high surface specificity after surface customization, and easy patterning for reagents. The purpose of this dissertation research is to make progress toward a chip scale integrated sensing system that realizes a high functionality optical system integration with a digital microfluidics platform for medical diagnostics and environmental monitoring.
This thesis describes the details of the design, fabrication, experimental measurement, and theoretical modeling of chip scale optical sensing systems integrated with electrowetting-on-dielectric digital microfluidic systems. Heterogeneous integration, a technology that integrates multiple optical thin film semiconductor devices onto arbitrary host substrates, has been utilized for this thesis. Three different integrated sensing systems were explored and realized. First, an integrated optical sensor based upon the heterogeneous integration of an InGaAs thin film photodetector with a digital microfluidic system was demonstrated. This integrated sensing system detected the chemiluminescent signals generated by a pyrogallol droplet solution mixed with H2O2 delivered by the digital microfluidic system.
Second, polymer microresonator sensors were explored. Polymer microresonators are useful components for chip scale integrated sensing because they can be integrated in a planar format using standard semiconductor manufacturing technologies. Therefore, as a second step, chip scale optical microdisk/ring sensors integrated with digital microfluidic systems were fabricated and measured. . The response of the microdisk and microring sensing systems to the change index of refraction, due to the glucose solutions in different concentrations presented by the digital microfluidic to the resonator surface, were measured to be 95 nm/RIU and 87nm/RIU, respectively. This is a first step toward chip-scale, low power, fully portable integrated sensing systems.
Third, a chip scale sensing system, which is composed of a planar integrated optical microdisk resonator and a thin film InGaAs photodetector, integrated with a digital microfluidic system, was fabricated and experimentally characterized. The measured sensitivity of this sensing system was 69 nm/RIU. Estimates of the resonant spectrum for the fabricated systems show good agreement with the theoretical calculations. These three systems yielded results that have led to a better understanding of the design and operation of chip scale optical sensing systems integrated with microfluidics.
Dissertation
You, Hong-Wei, and 游宏偉. "Fabrication of Microfluidic Blood Typing Chip and Its Optical Assay Analysis." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/58157142442207425122.
Full text中原大學
機械工程研究所
104
This study presents a novel microfluidic blood typing chip. The blood sample and reagents are driven by the capillary force, surface tension and gravity, without requiring any apparatuses for fluid manipulation. Chip fabrication was based on the photolithography process to produce a mold, followed by casting PDMS into the mold. Finally, the PDMD layer was bonded with a glass substrate. For blood typing measurement, an oscillator was used to reduce the precipitation time of blood agglutination. At the bottom of reaction vessel, a V-shape was designed. In addition, a vent connecting to the V-shaped vessel was used to release gas and prevent the occurrence of bubbles. Bioassay was based on the test protocol of manual polybrene (MP) technique. However, we used diluted whole blood in our experiments instead of centrifuged 3%-diluted red blood cells (RBC), which is used in the MP method. After the assay, the light intensity was detected to determine the result of blood typing.The experimental results show in this proposed chip the sample and reagents can be manipulated for the blood reaction without extra forces. The blood agglutination can be effectively precipitated. The diluted whole blood gives the same assay result as the centrifuged 3%-diluted RBC. We also concluded the optimal position for measurement in this proposed chip.
Charron, Luc. "Integrated Microfluidic Optical Manipulation Technique: Towards High Throughput Single Cell Analysis." Thesis, 2012. http://hdl.handle.net/1807/32681.
Full textLai, Chia-wei, and 賴嘉偉. "A Fiber Coupling and Cell Manipulating System Utilizing Microfluidic Devices for On-chip Dual-beam Optical Trap-and-Stretch." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/89334495899619154886.
Full text國立成功大學
工程科學系碩博士班
96
Fiber-dual-beam optical trap has been widely used for many applications such as the trapping or manipulation of micro-particles and cell biomechanics study. However, for these applications, precise alignment of a pair of optical fibers still remains a challenge. To tackle this issue, this study proposes a two-axis active optical-fiber manipulator for on-chip fiber alignment and optical dual beam trap applications. The chip comprising of a flow channel, air chambers, fiber channels, controllable moving walls and membrane microstructures were fabricated by using micro-electro-mechanical-systems (MEMS) technology. By adjusting air pressures to control the deflection of the pneumatic chambers placed orthogonal to and underneath the fiber channels, accurate alignment of a pair of co-axial optical-fibers, which was indicated by maximizing fiber-to-fiber coupling efficiency measured in real-time, has been achieved. A maximum displacement of a buried fiber as large as 13 μm at an applied pressure of 40 psi for one air chamber has been demonstrated. The maximum coupling efficiency for two single-mode optical-fibers facing each other at a distance of 200 μm was measured to be 4.1%. The multiple cells trapping manipulation by using the proposed chip also has been demonstrated. In addition, this study also developed a new microfluidic chip integrating the proposed fiber alignment device, cell transportation and pre-positioning systems utilizing MEMS techniques. The developed microfluidic chip is capable of delivering and pre-positioning cells in a predefined trapping zone, followed by manipulation of buried optical fibers and dual beam lasers for optical trapping, manipulation and stretcher. Experimental results showed that by integrating three micropumps connected in series, the cell samples were automatically delivered into the flow focusing area and then transported to the trapping zone. A single cell can be confined by micro-valves and then elevated towards the optical axis by a negative-DEP force operated at 20 Vp-p and 900 KHz. Finally, a red blood cell was successfully trapped, manipulated and stretched by active fiber manipulators and dual beam optical trap using the proposed microfluidic system. The developed microfluidic chip is promising for further applications that require trapping, manipulation and biomechanical analysis of a single cell or particle. Furthermore, the developed fibers alignment system is not only promising for applications requiring co-axial fibers for in-line optical analysis, but can also be easily integrated with other microfluidic systems such as capillary electrophoresis or micro flow cytometers for cell, protein, and DNA analysis.
Yang, Po Fu, and 楊博夫. "Optically-induced Cell Fusion on a Microfluidic Chip." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/13098944466659735919.
Full text國立清華大學
動力機械工程學系
103
Cell fusion is a critical course for all sort of biomedical applications including cell reprogramming, hybridoma formation, cancer immunotherapy, and tissue regeneration. It can be realized by using biological, chemical, or physical methods. However, efficiency and yields are limited by unstable cell contact and random cell pairings in traditional methods. Hence, improving cell contact and cell pairing are the two key factors to enhance efficiency and yields of cell fusion. This study therefore reported a new approach called optically-induced cell fusion (OICF) which integrates cell-pairing microstructures and optically-induced, localized electrical field to achieve precise cell fusion with high yields and high efficiency. By projecting light patterns on a photoconductive film (hydrogen-rich amorphous silicon, a-Si: H) coated on an indium-tin-oxide (ITO) glass while an alternating-current (AC) electric field was applied on the top and bottom ITO glasses, “virtual” electrodes would be constructed accordingly. In fact, this method could be used on several biomedical applications, including cell manipulation, cell separation, cell lysis and electroporation. Therefore, a locally enhanced electric field would be induced and the pairing cells could be precisely fused by the virtual electrodes. In this study, 57% cell paring rate and 87% fusion efficiency were achieved. Therefore, OICF is a promising method to succeed in cell fusion with high efficiency and high yields.
Li, Chang-Jyun, and 李昌駿. "A Biosensor based on the combination of microfluidic chips and D-type optical fibers." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/70587352559513940171.
Full text輔仁大學
物理學系碩士班
104
In this thesis, we combined the surface plasmon resonance (SPR) based side-polished D-type optical fiber sensor with microfluidic chips to measure the refractive indices of different liquids. The D-type OFS is based on the Kretchmann’ s configuration. By using the optical fiber to guide the light source to the side-polished region with deposited gold film, surface evanescent wave excites the gold film to attain the SPR phenomenon. A CO2 laser scriber was used to ablate polymethylmethacrylate (PMMA) substrates for making the microfluidic chips. These chips are cheap and easy to be fabricated. We combined the microfluidic chip with the D-type optical fiber to measure the spectrum peak of liquids with different refractive indices. We changed a variety of refractive indices for observing the difference in the spectrum peak and calculate the corresponding sensitivity. In our experiments, four sets of samples were measured: 1.ethanol mixed with water; 2.methanol mixed with water; 3.ethanol and methanol mixed with water; 4.different concentrations of glucose solution. Liquids with different refractive indices were flowed into the microfluidic chip for observing their effects on the spectrum peak. The sensitivity of the present OFS was calculated to be in the order of 10-6 RIU. We believed that, in the future, this microfluidic chip-integrated OFS can serve as a biosensor to monitor subtle changes in biological samples such as blood glucose, allergen, and biomolecular interactions.
Wu, Chun-Han, and 吳俊翰. "Using Optical Disc Process to Fabricate Microfluidic Chips for Optimization of Uniform Microcapsules Generation." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/81313794093823307144.
Full text國立交通大學
機械工程學系
99
This study presents a microfluidic platform fabricated by optical disc process for microcapsules generation. In the previous reports, the generation of microemulsion adopted microfluidic methods. The microfluidic chips were fabricated by casting process or by adopting CO2 laser engraving on plastic sheet. However, microfluidic chip of PDMS material is too soft, and can easily induce microchannel clogged. Furthermore, the cycle time for manufacturing the microfluidic substrate is over 3~4 hours. As for CO2 laser engraving, the surface of the microfluidic channels was too rough, uneven, deformed and different from the original designs. Besides, the size of the channels was limited by laser machine, and the cycle time for manufacturing the microfluidic substrate is over 10 minutes. The cycle time for both casting process and CO2 laser engraving process is too long. The long cycle time will be a drawback for mass production. Therefore, we present a new process of optical disc to fabricate microchannels of mold insert and adopt the micromolding technique of optical disc to prevent roughness, unevenness, deformation, and clog of microchannels. This paper is divided into three sections. (1) a design of microfluidic chip; (2) the fabrication of microfluidic mold insert and microfluidic chip; (3) generation of microemulsion droplet. In the design of microfluidic chip section, the depth of microchannels were 50/200 μm respectively and CFD-RC simulation software was adopted to simulate the motion of fluids and emulsions in microfluidic channels. In the fabrication of microfluidic mold insert, we propose a new process of optical disc to manufacture microfluidic mold insert. This new process can prevent the damage on the mirror plate of the mold caused by the traditional process of optical disc. The mold system is composed of a mold insert (stamper) holder and a vacuum system, which is used to join the mold insert with the mold. In this way, the time to change the stamper is drastically decreased. Therefore, it can improve the utility rate of injection molding. In the microfluidic chips of injection molding, we investigate the influence of controlled factors on the quality properties (depth of replication rate, width deviation, birefringence, tilt and surface roughness) of microfluidic chips. Those controlled factors include mold temperature, cylinder temperature, clamping force and injection speed. In the microemulsion generation, we use cross-junction microchannel to form uniform water-in-oil (w/o) emulsions. We prove that the size of these emulsion drops can be easily controlled by adjusting the ratio of disperser phase flow / continue phase flow. These emulsion drops, consisting of 1.5% (w/v) sodium alginate (Na-alginate), are then dripped into a solution containing 20% (w/v) calcium chloride (CaCl2) to create Ca-alginate microcapsules in an efficient manner. We apply Taguchi method to investigate the influence of controlled factors on the size of microemulsion drops which include the flow rate of dispersed phase flow, flow rate ratio, viscosity and surfactant concentration. The simulation results demonstrate that the bigger the flow rate ratio (continue phase flow /dispense phase flow) is, the smaller the size of microemulsion drops is, and the faster the speed of the generation microemulsion will become. In the fabrication mold insert, we successfully fabricate two layer of microfluidic mold insert, 300 um in thickness and the back side of mold insert is smooth and even. The proposed method will not damage the mirror of the mold. In the fabrication of microfluidic chips, we adopt DOE method to investigate the influence of controlled factors on the quality properties. The results indicated 2nd clamping force and injection speed have a greater effect on depth replication rate, width deviation and surface roughness. The injection speed and cylinder temperature have a greater effect on birefringence and the mold temperature has a greater effect on vertical deviation. The cycle time of injection molding is 4 seconds. It can be reduced to more than tenfold compared with the traditional ones. In the generation of microemulsion drops, we apply Taguchi method to investigate the influence of controlled factors on the size of microemulsion drops. The results show that the smallest size is 19.58 μm and coefficient of deviation of emulsion drop is 1.95%, the size of emulsion drop is 23.46% lower and the size deviation of drop is 20.73% lower than original design. We demonstrate that the Au nanoparticles and Immunoglobulins are encapsulated into Ca-alginate microcapsules. In this paper, we successfully devise the new method of optical disc process to fabricate microfluidic chips. The cycle time of microfluidic chips is 4 seconds. It is faster than traditional methods. The proposed microfluidic platform is capable of generating relatively uniform emulsions and has the advantages of active control of the emulsions diameter, a simple and low cost process, and a high throughput, highly efficient and suitable for mass production.
FA-HSUAN, CHU, and 朱法軒. "Development of an Optical Inspection System Used for Measuring Dimension of Bonded Microfluidic Chips." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/6wuvc6.
Full text國立臺灣科技大學
機械工程系
106
This study aims to develop an automated optical inspection system for measuring microchannel dimension. Research about microfluidic chip has flourished in recent years. There will be more and more related products which are important for dimension appearing. Therefore we developed a non-destructive and non-contact measurement for bonded microfluidic chip to verify whether the microchannel dimension meets the tolerance specification. The data measured with measurement system we’ve developed is compared with the reference value measured with toolmaker microscope. The error obtained after comparing reaches ± 2 %.
Ho, Stephen. "Femtosecond Laser Microfabrication of Optofluidic Lab-on-a-chip with Selective Chemical Etching." Thesis, 2013. http://hdl.handle.net/1807/65508.
Full textJhuang, Yi-Chi, and 莊逸琦. "Study on Optimal Process of Microfluidic Chip by Genetic Algorithm and Differential Evolution Algorithm and Grey Theory." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/8k2jc3.
Full text國立臺北科技大學
工業工程與管理系碩士班
102
In recent years, the emerging technologies such as DNA sequencing, protein analysis and micro-electronics draw a lot of research attention. The application of the micro-electronics is among the most important ones. The sensitivity of machine has reached a certain level. Due to lengthy inspecting time, difficulty of carrying, expensive cost, some inspecting devices are not universal. The concept of miniaturization becomes very critical to the development of micro-electronic technology which integrates the sampling, pretreatment, detachment, testing process into the micro-fluidic chip that gives an impetus to the sample to move between the micro channels of the parts to detect by centrifugal force and impressed voltage. The main advantage of micro-fluidic chip is to increase the performance, quality, reliabilityand reduce the sample consumption and cost. The applications of micro-fluidic chip include new medicine development, disease examination, DNA sequencing, protein analysis, infection cause of disease, blood sieves examines, environment examination, food examination and so on. A lot of cost may happen if the micro-fluidic chip manufacturing process does not result in high quality and yield rate.In this project, we proposed a model which tries to optimize the parameters of the manufacturing process of micro-fluidic chips.The Taguchi method is used to reduce the number of experiments. Genetic algorithm, differential evolution algorithm and grey theory are utilized to optimize the parameters of manufacturing process. The result of gray relational analysis obtains the optimize the parameters of the manufacturing process of micro-fluidic chips and the effect of each parameters.the most important parameters of the manufacturing process of micro-fluidic chips is the embossing temperature. The result of the algorithms are apparently improved the result of the original process.
Hsiao, Yu-Chun, and 蕭宇君. "Automatic cell fusion using optically-induced dielectrophoresis and optically-induced locally-enhanced electric field on a structure-free microfluidic chip." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/8yg6d8.
Full text