Log in

Relevant bibliographies by topics / Microdissection / Journal articles

To see the other types of publications on this topic, follow the link: Microdissection.

Journal articles on the topic 'Microdissection'

Author: Grafiati

Published: 4 June 2021

Last updated: 7 July 2024

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Microdissection.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Qin, Dahui, Zhong Zheng, Shanxiang Shen, Prudence Smith, and Farah K. Khalil. "Necessity of Microdissecting Different Tumor Components in Pulmonary Tumor Pyrosequencing." BioMed Research International 2016 (2016): 1–5. http://dx.doi.org/10.1155/2016/8759267.

Full text

Abstract:

Microdissection is a useful method in tissue sampling prior to molecular testing. Tumor heterogeneity imposes new challenges for tissue sampling. Different microdissecting methods have been employed in face of such challenge. We improved our microdissection method by separately microdissecting the morphologically different tumor components. This improvement helped the pyrosequencing data analysis of two specimens. One specimen consisted of both adenocarcinoma and neuroendocrine components. When both tumor components were sequenced together for KRAS (Kirsten rat sarcoma viral oncogene homolog) gene mutations, the resulting pyrogram indicated that it was not a wild type, suggesting that it contained KRAS mutation. However, the pyrogram did not match any KRAS mutations and a conclusion could not be reached. After microdissecting and testing the adenocarcinoma and neuroendocrine components separately, it was found that the adenocarcinoma was positive for KRAS G12C mutation and the neuroendocrine component was positive for KRAS G12D mutation. The second specimen consisted of two morphologically different tumor nodules. When microdissected and sequenced separately, one nodule was positive for BRAF (v-raf murine sarcoma viral oncogene homolog B1) V600E and the other nodule was wild type at the BRAF codon 600. These examples demonstrate that it is necessary to microdissect morphologically different tumor components for pyrosequencing.

APA, Harvard, Vancouver, ISO, and other styles

2

Tangrea, Michael A., Rodrigo F. Chuaqui, John W. Gillespie, Mamoun Ahram, Gallya Gannot, Benjamin S. Wallis, Carolyn J. M. Best, et al. "Expression Microdissection." Diagnostic Molecular Pathology 13, no. 4 (December 2004): 207–12. http://dx.doi.org/10.1097/01.pdm.0000135964.31459.bb.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Pixell, I. I. "Microdissection Laser." Biofutur 1999, no. 193 (October 1999): 52. http://dx.doi.org/10.1016/s0294-3506(00)87141-0.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Hunt, Jennifer L., and Sydney D. Finkelstein. "Microdissection Techniques for Molecular Testing in Surgical Pathology." Archives of Pathology & Laboratory Medicine 128, no. 12 (December 1, 2004): 1372–78. http://dx.doi.org/10.5858/2004-128-1372-mtfmti.

Full text

Abstract:

Abstract Objective.—To describe the techniques for microdissection of paraffin-embedded and frozen tissue sections for the use in molecular applications. Data Sources.—Original research papers and review papers and the authors' personal experiences. Data Synthesis.—Manual and laser-capture microdissection are described in detail, with specific protocols for sample preparation and instructions for performing the microdissection. A section addressing frequently asked questions is also included. Conclusions.—Microdissection is a technique that is very useful both in the research setting and for clinical molecular testing in paraffin-embedded tissue samples. The available techniques range from simple and inexpensive (manual microdissection) to complex and expensive (laser-capture microdissection). All of the techniques, however, require the user to be familiar with microscopy and histology.

APA, Harvard, Vancouver, ISO, and other styles

5

Emmert-Buck, Michael R., Robert F. Bonner, Paul D. Smith, Rodrigo F. Chuaqui, Zhengping Zhuang, Seth R. Goldstein, Rhonda A. Weiss, and Lance A. Liotta. "Laser Capture Microdissection." Science 274, no. 5289 (November 8, 1996): 998–1001. http://dx.doi.org/10.1126/science.274.5289.998.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Espina, Virginia, Julia D. Wulfkuhle, Valerie S. Calvert, Amy VanMeter, Weidong Zhou, George Coukos, David H. Geho, Emanuel F. Petricoin, and Lance A. Liotta. "Laser-capture microdissection." Nature Protocols 1, no. 2 (June 27, 2006): 586–603. http://dx.doi.org/10.1038/nprot.2006.85.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Tresser, N., M. Ouezado, L. Whitney, K. Becker, R. Bonner, M. Emmert-Buck, and L. Liotta. "LASER CAPTURE MICRODISSECTION." Journal of Neuropathology and Experimental Neurology 57, no. 5 (May 1998): 505. http://dx.doi.org/10.1097/00005072-199805000-00164.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Jensen, Ellen. "Laser-Capture Microdissection." Anatomical Record 296, no. 11 (October 4, 2013): 1683–87. http://dx.doi.org/10.1002/ar.22791.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Tsai, Cheng-Han, I.-Shen Huang, Wei-Jen Chen, Li-Hua Li, Eric Yi-Hsiu Huang, and William J. Huang. "Repeat Microdissection Testicular Sperm Extraction in Azoospermic Men with Nonmosaic Klinefelter Syndrome." Andrologia 2023 (May 23, 2023): 1–7. http://dx.doi.org/10.1155/2023/3955704.

Full text

Abstract:

Introduction. To investigate the predictive factors for successful repeat microdissection testicular sperm extraction attempts in patients with Klinefelter syndrome. Methods. A total of 28 azoospermic men with nonmosaic Klinefelter syndrome who have received microdissection testicular sperm extraction twice with successful initial microdissection testicular sperm extraction attempts in our institute were studied. Outcome variables (age, serum follicle-stimulating hormone, luteinizing hormone, testosterone, prolactin, and estradiol) of azoospermic men with nonmosaic Klinefelter syndrome and a successful 2nd surgical sperm retrieval attempt (group A) were compared to those with an unsuccessful 2nd sperm retrieval attempt (group B). Results. Twenty-one patients (75%) had successful sperm recovery at the 2nd microdissection testicular sperm extraction attempt. The mean testosterone level at baseline and before the 1st microdissection testicular sperm extraction attempt was higher in group A than in group B (2.7 vs. 0.9 ng/mL, p < 0.01 , and 3.9 vs. 1.1 ng/mL, p = 0.02 ). Receiver operating characteristic curve analysis identified the threshold baseline testosterone concentration (1.5 ng/mL) of patients with Klinefelter syndrome in predicting successful 2nd sperm retrieval attempts and revealed positive and negative predictive values of 94.44% and 60%, respectively. Conclusion. Azoospermic men with Klinefelter syndrome presenting with low testosterone levels and successful sperm recovery during the first microdissection testicular sperm extraction procedure are unlikely to retrieve sperm on the 2nd microdissection testicular sperm extraction attempt. Hence, these patients should be properly counseled before sperm retrieval.

APA, Harvard, Vancouver, ISO, and other styles

10

Kumar, Pramod, and Virendra Singh. "Modified microdissection electrocautery needle." National Journal of Maxillofacial Surgery 5, no. 2 (2014): 243. http://dx.doi.org/10.4103/0975-5950.154849.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Flannigan, Ryan, Phil V. Bach, and Peter N. Schlegel. "Microdissection testicular sperm extraction." Translational Andrology and Urology 6, no. 4 (August 2017): 745–52. http://dx.doi.org/10.21037/tau.2017.07.07.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

Majer, Anna, and Stephanie A. Booth. "Microdissection and transcriptional profiling." Prion 8, no. 1 (January 2014): 67–74. http://dx.doi.org/10.4161/pri.27729.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Espina, Virginia, Michael Heiby, Mariaelena Pierobon, and Lance A. Liotta. "Laser capture microdissection technology." Expert Review of Molecular Diagnostics 7, no. 5 (September 2007): 647–57. http://dx.doi.org/10.1586/14737159.7.5.647.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Fend, Falko, Marcus Kremer, Katja Specht, and Leticia Quintanilla-Martinez. "Laser Microdissection in Hematopathology." Pathology - Research and Practice 199, no. 6 (January 2003): 425–30. http://dx.doi.org/10.1078/0344-0338-00441.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Grant, Kenneth, and W. Gray Jerome. "Laser Capture Microdissection as an Aid to Ultrastructural Analysis." Microscopy and Microanalysis 8, no. 3 (June 2002): 170–75. http://dx.doi.org/10.1017/s143192760202010x.

Full text

Abstract:

Laser capture microdissection uses a microscope to identify specific cells for microdissection and then a laser-sensitive plastic to capture and remove the cells from their substrate. This efficient capture method was originally developed to capture cells for genetic analysis. However, it has also been used to capture cells for proteonomic analysis. In this article, we extend the uses of laser-capture microdissection by reporting a method for preparing captured cells for ultrastructural analysis by transmission electron microscopy. Cells prepared by our methodology show good fine structure preservation and are easily sectioned by standard ultramicrotomy.

APA, Harvard, Vancouver, ISO, and other styles

16

Huang, Haibo, Yifan Pan, Yan Pang, Hao Shen, Xiwei Gao, Yichen Zhu, Liguo Chen, and Lining Sun. "Piezoelectric Ultrasonic Biological Microdissection Device Based on a Novel Flexure Mechanism for Suppressing Vibration." Micromachines 12, no. 2 (February 13, 2021): 196. http://dx.doi.org/10.3390/mi12020196.

Full text

Abstract:

Biological microdissection has a wide range of applications in the field of molecular pathology. The current laser-assisted dissection technology is expensive. As an economical microdissection method, piezoelectric ultrasonic microdissection has broad application prospects. However, the performance of the current piezoelectric ultrasonic microdissection technology is unsatisfactory. This paper aims to solve the problems of the low dissecting precision and excessive wear of the dissecting needle caused by the harmful lateral vibration of the present piezoelectric ultrasonic microdissection device. A piezoelectric ultrasonic microdissection device based on a novel flexure mechanism is proposed. By analyzing the flexure hinge flexibility, the type of flexure beam and the optimal design parameters are determined. Through harmonic response simulation analysis, the newly designed microdissection device with a vibration-suppressing mechanism achieves the best vibration effect when the driving frequency is 28 kHz. Under this driving frequency, the lateral vibration suppression effect is improved by 68% compared to the traditional effect without vibration suppression. Then, based on 3D printing technology, a prototype of a novel microdissection device is produced, and its performance is tested. Experiments on dissecting needle vibration tests show that the flexure mechanism does indeed suppress the lateral vibration of the needle tip. We conducted various tissue dissection experiments on paraffin tissue sections. First, we determine the optimal dissecting parameters (driving voltage, frequency, feed speed, cutting angle) of the new equipment through various parameter dissecting experiments. Then, we adopt these optimal dissecting parameters to perform three kinds of dissecting experiments on mouse tissue paraffin section (liver, lung, bone), dissecting experiments on tissue sections of different thicknesses (3 μm, 4 μm, 5 μm), sampling and extraction experiments on complete tissue. The new device has a better dissecting performance for paraffin tissue sections below a 5 μm thickness and can complete various dissecting tasks. Finally, we compare the wear of the dissecting needles of the new and old devices after the same dissecting tasks. The results prove that the suppression of harmful lateral vibration not only significantly improves the dissecting effect but also increases the service life and durability of the dissecting needle, which is beneficial for reducing the equipment costs.

APA, Harvard, Vancouver, ISO, and other styles

17

He, Wei, Yagang Liu, Moyra Smith, and Michael W. Berns. "Laser Microdissection for Generation of a Human Chromosome Region-specific Library." Microscopy and Microanalysis 3, no. 1 (January 1997): 47–52. http://dx.doi.org/10.1017/s1431927697970033.

Full text

Abstract:

Abstract: A human chromosome pq34 region-specific, microdissected library was constructed by using laser microdissection techniques. This library contains over 10,000 clones with an average insert size of 450 bp. It has greater coverage of the dissected chromosome region as compared with the needle-dissected chromosome 9q34 library. The laser microdissection technique provides more accurate chromosome targeting and easier operation than existing needle microdissection techniques. To simplify the procedure for chromosome microdissection and chromosome fragment collection, a trapping-cutting system was developed. This technique involves the use of two trapping beams which hold a single chromosome in suspension, and a third cutting beam, which dissects the immobilized chromosome. A collection chamber allowing for the fast collection of dissected chromosome fragments needs to be developed. However, DNA can be cloned from trapped chromosome fragments with an insert size comparable to that of both needle-cut and laser-cut chromosomes.

APA, Harvard, Vancouver, ISO, and other styles

18

Bahary, Nathan, Donna E. McGraw, Rebecca Shilling, and Jeffrey M. Friedman. "Microdissection and Microcloning of Mid-Chromosome 4: Genetic Mapping of 41 Microdissection Clones." Genomics 16, no. 1 (April 1993): 113–22. http://dx.doi.org/10.1006/geno.1993.1148.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Kim, Chong Jai. "Laser-based Microdissection of Cell." Journal of the Korean Medical Association 43, no. 4 (2000): 353. http://dx.doi.org/10.5124/jkma.2000.43.4.353.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

Cannizzaro, L. A. "Chromosome microdissection: a brief overview." Cytogenetic and Genome Research 74, no. 3 (1996): 157–60. http://dx.doi.org/10.1159/000134407.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

HaiJin, Yi, Guo Weiwei, Chen Lei, Wu Na, Li JiaNa, Ren LiLi, and Yang ShiMing. "Microdissection of Miniature Pig Ear." Journal of Otology 8, no. 2 (December 2013): 91–96. http://dx.doi.org/10.1016/s1672-2930(13)50019-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

Hudock, Teresa A., Andrew A. Lackner, and Deepak Kaushal. "Microdissection approaches in tuberculosis research." Journal of Medical Primatology 43, no. 5 (August 28, 2014): 294–97. http://dx.doi.org/10.1111/jmp.12141.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Chokechanachaisakul, Uraiwan, Tomoatsu Kaneko, Takashi Okiji, Reika Kaneko, Hideaki Suda, and Jacques E. Nör. "Laser Capture Microdissection in Dentistry." International Journal of Dentistry 2010 (2010): 1–8. http://dx.doi.org/10.1155/2010/592694.

Full text

Abstract:

Laser capture microdissection (LCM) allows for the microscopic procurement of specific cell types from tissue sections that can then be used for gene expression analysis. According to the recent development of the LCM technologies and methodologies, the LCM has been used in various kinds of tissue specimens in dental research. For example, the real-time polymerase-chain reaction (PCR) can be performed from the formaldehyde-fixed, paraffin-embedded, and immunostained sections. Thus, the advance of immuno-LCM method allows us to improve the validity of molecular biological analysis and to get more accurate diagnosis in pathological field in contrast to conventional LCM. This paper is focused on the presentation and discussion of the existing literature that covers the fields of RNA analysis following LCM in dentistry.

APA, Harvard, Vancouver, ISO, and other styles

24

Greulich, Karl Otto. "Chromosome microtechnology: microdissection and microcloning." Trends in Biotechnology 10 (1992): 48–51. http://dx.doi.org/10.1016/0167-7799(92)90168-u.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Böhm, Christine, Dieter Newrzella, and Oliver Sorgenfrei. "Laser microdissection in CNS research." Drug Discovery Today 10, no. 17 (September 2005): 1167–74. http://dx.doi.org/10.1016/s1359-6446(05)03555-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Gupta, S. K., A. G. Douglas-Jones, and J. M. Morgan. "Microdissection of stained archival tissue." Molecular Pathology 50, no. 4 (August 1, 1997): 218–20. http://dx.doi.org/10.1136/mp.50.4.218.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Fend, F. "Laser capture microdissection in pathology." Journal of Clinical Pathology 53, no. 9 (September 1, 2000): 666–72. http://dx.doi.org/10.1136/jcp.53.9.666.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

NOGUCHI, MASAYUKI. "Tissue microdissection and molecular diagnosis." Juntendo Medical Journal 46, no. 4 (2001): 416–22. http://dx.doi.org/10.14789/pjmj.46.416.

Full text

APA, Harvard, Vancouver, ISO, and other styles

29

Hsiao, W., R. Ramasamy, J. A. Ricci, and P. N. Schlegel. "Microdissection TESE: the learning curve." Fertility and Sterility 94, no. 4 (September 2010): S17—S18. http://dx.doi.org/10.1016/j.fertnstert.2010.07.068.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

Vila-Coro, Antonio Aguirre. "Vascular Microdissection in Strabismus Surgery." Archives of Ophthalmology 108, no. 7 (July 1, 1990): 1034. http://dx.doi.org/10.1001/archopht.1990.01070090136056.

Full text

APA, Harvard, Vancouver, ISO, and other styles

31

Fend, Falko, Marcus Kremer, and Leticia Quintanilla-Martinez. "Laser Capture Microdissection: Methodical Aspects and Applications with Emphasis on Immuno-Laser Capture Microdissection." Pathobiology 68, no. 4-5 (2000): 209–14. http://dx.doi.org/10.1159/000055925.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Zheng, Yi, Ding-Ming Li, Fu-Ping Li, Xiao-Hui Jiang, Luo Yang, Rui Qu, Heng-Zhou Bai, Gui-Cheng Zhao, and Kun Tian. "Case report: remedial microdissection testicular sperm extraction after onco-microdissection testicular sperm extraction failure." Medicine 103, no. 8 (February 23, 2024): e37201. http://dx.doi.org/10.1097/md.0000000000037201.

Full text

Abstract:

Background: Testicular cancer (TC) mostly occurs in men aged 14 to 44. Studies have shown that TC seriously damages male fertility, and 6% to 24% of patients with TC were even found to suffer from azoospermia when they are diagnosed. At present, some studies have pointed out that onco-microdissection testicular sperm extraction (mTESE) can extract sperm from tumor testicles. However, there are almost no reports on remedial measures after onco-mTESE failure. Given the valuable opportunity for fertility preservation in patients with TC and azoospermia, it is necessary to provide effective remedial methods for patients with failed onco-mTESE. Methods: Two young men, who were diagnosed with TC and also found to have azoospermia, tried onco-mTESE while undergoing radical orchiectomy for fertility preservation. However, sperm extraction failed in both patients. Subsequently, the isolated testicular tissue of the patient in case 1 suffered from TC again, and the patient in case 2 was scheduled to receive multiple cycles of gonadotoxic chemotherapy. Because both had a plan to have a birth in the future, we performed remedial mTESE. Results: Sperm was successfully extracted from both patients. The patient recovered well, without complications. The patient couple in case 1 underwent 1 intracytoplasmic sperm injection (ICSI) cycle but did not achieve clinical pregnancy. Conclusions: There is still an opportunity to extract sperm successfully using onco-mTESE, despite the difficulty of fertility preservation in TC patients with azoospermia. If sperm extraction from the tumor testis fails, implementing remedial mTESE as early as possible would likely preserve the last chance of fertility for these patients.

APA, Harvard, Vancouver, ISO, and other styles

33

Hsiao, Wayland, Peter J. Stahl, E. Charles Osterberg, Edward Nejat, Gianpiero D. Palermo, Zev Rosenwaks, and Peter N. Schlegel. "Successful Treatment of Postchemotherapy Azoospermia With Microsurgical Testicular Sperm Extraction: The Weill Cornell Experience." Journal of Clinical Oncology 29, no. 12 (April 20, 2011): 1607–11. http://dx.doi.org/10.1200/jco.2010.33.7808.

Full text

Abstract:

Purpose Advances in chemotherapy have led to greater longevity and paternity may be an important consideration for postchemotherapy survivors of childhood cancers. While traditionally considered sterile, men who are azoospermic after chemotherapy can be treated with microdissection testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI). Patients and Methods Oncologic data, pretreatment hormone profiles, testicular histology, and outcomes of microdissection TESE-ICSI were reviewed. ICSI was performed in a programmed in vitro fertilization cycle using fresh spermatozoa. Embryos were transferred into the uterine cavity on the third day after microinjection. Results Eighty-four microdissection TESE procedures were performed in 73 patients. The mean time elapsed since chemotherapy was 18.6 years (range, 1 to 34 years). Spermatozoa were retrieved in 37% of patients and in 42.9% of overall procedures. A 57.1% fertilization rate (per injected oocyte) was achieved with ICSI allowing a 50% clinical pregnancy rate with a live birth rate of 42% overall. There were 15 deliveries, with a total of 20 children born. Hypospermatogenesis seen on preoperative biopsy was associated with 100% sperm retrieval while exposure to alkylating agents resulted in a significantly lower sperm retrieval rate. Patients with testicular cancer had the highest sperm retrieval rates while patients previously treated for sarcoma had the lowest retrieval rates. Conclusion To our knowledge, this represents the largest series of postchemotherapy microdissection TESE-ICSI to date. Sperm were retrieved in 37% of patients despite a prevalence of Sertoli cell–only pattern on preoperative biopsy. Although prechemotherapy sperm cryopreservation is recommended, treatment with microdissection TESE and ICSI are effective treatment options for many azoospermic men after chemotherapy.

APA, Harvard, Vancouver, ISO, and other styles

34

Llambí, S., and M. V. Arruga. "Molecular approach of the fragile chromosomal region Xq31-34 in cattle (Bos taurus) by microdissection and DOP-PCR." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 60, no. 4 (August 2008): 926–31. http://dx.doi.org/10.1590/s0102-09352008000400023.

Full text

Abstract:

Fragile sites (FS) are chromosomal regions where the normal compactation of chromatine is not observed. FRAXA (Fra Xq27.3, X sexual chromosome) is one of the most studied FS in humans. FRAXA is an expansion of the trinucleotide CGG located in the gene FMR-1. In cattle, sites of chromosomal fragility were reported in BTAX, associated with different pathologies and fertility impairment. Chromosomal microdissection has became a valuable tool for isolating chromatine fragments. In this work, it was combined the chromosomal microdissection technique with DOP-PCR in order to carry out a molecular analysis of the fragile chromosomal region BTAXq31-34. In that region, polymorphic DNA-RAPD sequences (GC rich) are present and sequences of the gene FMR-1 are missing. The results showed the usefulness of the microdissection-DOP-PCR technique for molecular characterization of fragile chromosomal sites in cattle.

APA, Harvard, Vancouver, ISO, and other styles

35

Wong, Chui E., Mohan B. Singh, and Prem L. Bhalla. "Sample preparation for laser-microdissection of soybean shoot apical meristem." International Journal of Plant Biology 3, no. 1 (October 16, 2012): 3. http://dx.doi.org/10.4081/pb.2012.e3.

Full text

Abstract:

The shoot apical meristem houses stem cells responsible for the continuous formation of aerial plant organs including leaves and stems throughout the life of plants. Laser-microdissection in combination with high-throughput technology such as next generation sequencing permits an in-depth analysis of molecular events associated with specific cell type of interest. Sample preparation is the most critical step in ensuring good quality RNA to be extracted from samples following laser-microdissection. Here, we optimized the sample preparation for a major legume crop, soybean. We used Farmer’s solution as a fixative and paraffin as the embedding medium for soybean shoot apical meristem tissue without the use of any specialized equipment. Shorter time for tissue fixation (two days) was found to be critical for the preservation of RNA in soybean shoot apical meristem. We further demonstrated the utility of this method for different tissues derived from soybean and rice. The method outlined here shall facilitate studies on crop plants involving laser-microdissection.

APA, Harvard, Vancouver, ISO, and other styles

36

Dahse, Regine, and Hartwig Kosmehl. "CELL SEPARATION AND GENE EXPRESSION ANALYSIS IN A TUMOR-STROMA INTERACTION MODEL." Image Analysis & Stereology 23, no. 3 (May 3, 2011): 153. http://dx.doi.org/10.5566/ias.v23.p153-157.

Full text

Abstract:

A novel technique for co-culturing and separating fibroblasts and carcinoma cells in a 2-D model of tumorstroma interaction is presented. The methodology is based on cell co-cultivation on an 1.35 μm thin membrane followed by rapid immunostaining and microdissection of the different cell compartments using a laser microdissection system (P.A.L.M. Microlaser Technologies AG, Germany). For identifying the tumor cell compartment, immunolabeling for a marker that is expressed only in epithelial tumor cells is performed. The RNA quality from the microdissected co-cultured cells was successfully proved by RT-PCR for a housekeeping gene transcript and for the laminin gamma 2 chain gene transcript used before in the tumor cell immunostaining. Laminin cDNA was amplificable only in tumor cells and not in the co-cultivated fibroblasts indicating no cell-cross-contamination during microdissection. Microdissected tumor and stroma cells from the presented membrane based co-culture model can be used for gene expression profiling and DNA based analysis in the investigation of tumor-stroma interactions.

APA, Harvard, Vancouver, ISO, and other styles

37

Nambiar, P. R., S. R. Boutin, R. Raja, and D. W. Rosenberg. "Global Gene Expression Profiling: A Complement to Conventional Histopathologic Analysis of Neoplasia." Veterinary Pathology 42, no. 6 (November 2005): 735–52. http://dx.doi.org/10.1354/vp.42-6-735.

Full text

Abstract:

Transcriptional profiling of entire tumors has yielded considerable insight into the molecular mechanisms of heterogeneous cell populations within different types of neoplasms. The data thus acquired can be further refined by microdissection methods that enable the analyses of subpopulations of neoplastic cells. Separation of the various components of a neoplasm (i.e., stromal cells, inflammatory infiltrates, and blood vessels) has been problematic, primarily because of a paucity of tools for accurate microdissection. The advent of laser capture microdissection combined with powerful tools of linear amplification of RNA and high-throughput microarray-based assays have allowed the transcriptional mapping of intricate and highly complex networks within pure populations of neoplastic cells. With this approach, specific “molecular signatures” can be assigned to tumors of distinct or even similar histomorphology, thereby aiding the desired objective of pattern recognition, tumor classification, and prognostication. This review highlights the potential benefits of global gene expression profiling of tumor cells as a complement to conventional histopathologic analyses.

APA, Harvard, Vancouver, ISO, and other styles

38

ZHOU, JI-BO, SHENGFANG GE, PING GU, DUO PENG, GUO-FU CHEN, MIAO-ZHEN PAN, and JIA QU. "Microdissection of guinea pig extraocular muscles." Experimental and Therapeutic Medicine 2, no. 6 (2011): 1183–85. http://dx.doi.org/10.3892/etm.2011.341.

Full text

APA, Harvard, Vancouver, ISO, and other styles

39

Sujatha, Govindarajan, and Jayanandan Muruganandhan. "Laser Capture Microdissection in Oral Cancer." Journal of Contemporary Dental Practice 19, no. 5 (2018): 475–76. http://dx.doi.org/10.5005/jp-journals-10024-2286.

Full text

APA, Harvard, Vancouver, ISO, and other styles

40

Wright, Charles G., and William L. Meyerhoff. "Microdissection in Human Temporal Bone Morphology." Annals of Otology, Rhinology & Laryngology 98, no. 12_suppl (December 1989): 25–28. http://dx.doi.org/10.1177/0003489489098s1213.

Full text

APA, Harvard, Vancouver, ISO, and other styles

41

Lechpammer, Mirna. "LASER CAPTURE MICRODISSECTION: METHODS AND PROTOCOLS." Shock 25, no. 4 (April 2006): 426–27. http://dx.doi.org/10.1097/01.shk.0000223839.51394.1c.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Goldstein, Seth R., Philip G. McQueen, and Robert F. Bonner. "Thermal modeling of Laser Capture Microdissection." Applied Optics 37, no. 31 (November 1, 1998): 7378. http://dx.doi.org/10.1364/ao.37.007378.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Brooks, Lionel, Josh Strable, Xiaolan Zhang, Kazuhiro Ohtsu, Ruilian Zhou, Ananda Sarkar, Sarah Hargreaves, et al. "Microdissection of Shoot Meristem Functional Domains." PLoS Genetics 5, no. 5 (May 8, 2009): e1000476. http://dx.doi.org/10.1371/journal.pgen.1000476.

Full text

APA, Harvard, Vancouver, ISO, and other styles

44

Métézeau, Philippe, and Mustapha Bensaasa. "La microdissection de chromosomes par laser." Biofutur 1997, no. 165 (March 1997): 13A. http://dx.doi.org/10.1016/s0294-3506(97)87634-x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Murray, Graeme I. "An overview of laser microdissection technologies." Acta Histochemica 109, no. 3 (June 2007): 171–76. http://dx.doi.org/10.1016/j.acthis.2007.02.001.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Santegoeds, R. G. C., Y. Yakkioui, A. Jahanshahi, G. Raven, J. J. Van Overbeeke, A. Herrler, and Y. Temel. "Notochord isolation using laser capture microdissection." Journal of Chemical Neuroanatomy 80 (March 2017): 37–43. http://dx.doi.org/10.1016/j.jchemneu.2016.12.004.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Samuel, Raheel, Odgerel Badamjav, Kristin E. Murphy, Darshan P. Patel, Jiyoung Son, Bruce K. Gale, Douglas T. Carrell, and James M. Hotaling. "Microfluidics: The future of microdissection TESE?" Systems Biology in Reproductive Medicine 62, no. 3 (April 22, 2016): 161–70. http://dx.doi.org/10.3109/19396368.2016.1159748.

Full text

APA, Harvard, Vancouver, ISO, and other styles

48

Dabaja, Ali A., and Peter N. Schlegel. "Microdissection testicular sperm extraction: an update." Asian Journal of Andrology 15, no. 1 (December 17, 2012): 35–39. http://dx.doi.org/10.1038/aja.2012.141.

Full text

APA, Harvard, Vancouver, ISO, and other styles

49

Fominaya, A., C. Linares, Y. Loarce, and E. Ferrer. "Microdissection and microcloning of plant chromosomes." Cytogenetic and Genome Research 109, no. 1-3 (2005): 8–14. http://dx.doi.org/10.1159/000082376.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

Chimenti, Cristina, Maurizio Pieroni, Andrea Russo, Patrizio Sale, Matteo A. Russo, Attilio Maseri, and Andrea Frustaci. "Laser Microdissection in Clinical Cardiovascular Research." Chest 128, no. 4 (October 2005): 2876–81. http://dx.doi.org/10.1378/chest.128.4.2876.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!