Relevant bibliographies by topics / Microcosm Inoculation

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Academic literature on the topic 'Microcosm Inoculation'

Author: Grafiati
Published: 10 March 2023

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Journal articles on the topic "Microcosm Inoculation"

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Sayel, Hanane, Nezha Tahri Joutey, Wifak Bahafid, and Naima El Ghachtouli. "Chromium Resistant Bacteria: Impact on Plant Growth in Soil Microcosm." Archives of Environmental Protection 40, no. 2 (July 8, 2014): 81–89. http://dx.doi.org/10.2478/aep-2014-0017.

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Abstract Three chromium resistant bacterial strains, Pseudomonas fluorescens PF28, Enterobacter amnigenus EA31 and Enterococcus gallinarum S34 isolated from tannery waste contaminated soil were used in this study. All strains could resist a high concentration of K2Cr2O7 that is up to 300 mg/L. The effect of these strains on clover plants (Trifolium campestre) in the presence of two chromium salts CrCl3 and K2Cr2O7 was studied in soil microcosm. Application of chromium salts adversely affected seed germination, root and shoot length. Bacterial inoculation improved the growth parameters under chromate stress when compared with non inoculated respective controls. There was observed more than 50% reduction of Cr(VI) in inoculated soil microcosms, as compared to the uninoculated soil under the same conditions. The results obtained in this study are significant for the bioremediation of chromate pollution.
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Shintani, Masaki, Tadafumi Horisaki, Hisakazu Yamane, Moriya Ohkuma, and Hideaki Nojiri. "Evolution of the IncP-7 carbazole-degradative plasmid pCAR1 improves survival of its host Pseudomonas fluorescens Pf0-1 in artificial water microcosms." Microbiology 157, no. 8 (August 1, 2011): 2276–86. http://dx.doi.org/10.1099/mic.0.049064-0.

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In our previous study, Pseudomonas fluorescens Pf0-1L, harbouring the IncP-7 carbazole-degradative plasmid pCAR1 : : rfp, was shown to be undetectable within 5 days post-inoculation in carbazole-contaminated artificial freshwater microcosms containing several plasmid-free bacteria in addition to Pf0-1L(pCAR1 : : rfp). Fourteen days after the inoculation, carbazole degraders become detectable. Here, we revealed that these isolates were not pCAR1 transconjugants, but Pf0-1L(pCAR1 : : rfp) mutants, based on RFLP and BOX-A1R-based repetitive extragenic palindromic-PCR (BOX-PCR) analysis. Notably, the mutants displayed more rapid initiation of carbazole degradation than the parent strain Pf0-1L(pCAR1 : : rfp). The mutants were unable to degrade anthranilate due to a 163 bp deletion in the antA gene, which was overcome by their transformation with a wild-type antABC-expressing plasmid. Quantitative RT-PCR analysis indicated that the transcriptional induction of carbazole-, anthranilate- and catechol-degradative genes was comparable in both parent and mutant strains. The deletion mutants became dominant in the artificial water microcosm. The mutation caused anthranilate to accumulate instead of catechol, a toxic compound for the parent strain, and may be beneficial to host survival in artificial microcosms.
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Humphreys, Gavin J., and Andrew J. McBain. "Continuous culture of sessile human oropharyngeal microbiotas." Journal of Medical Microbiology 62, no. 6 (June 1, 2013): 906–16. http://dx.doi.org/10.1099/jmm.0.055806-0.

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The microbiota of the human oropharynx plays an important role in health through involvement in the aetiology of infection and the carriage of adventitious pathogens. Despite this, there are few models available for the preclinical assessment of novel antimicrobials directed to the human throat. We have profiled bacterial consortia sampled from the palatine tonsil and posterior pharyngeal wall microbiotas of healthy adult volunteers (n = 10) using differential culture and 16S rRNA gene sequencing, together with PCR-denaturing gradient gel electrophoresis. The data generated were used to assess the validity of an oropharyngeal microcosm system based on replicated constant-depth film fermenters (CDFFs; n = 5), which were continuously fed using an artificial airway surface liquid. Developed microcosms exhibited significant homology to ex situ consortia according to principal components analysis, whilst compositional reproducibility was apparent in replicated models for tonsillar and pharyngeal inocula. Differential viable count data and Shannon–Weiner diversity indices indicated that representative tonsil and pharyngeal model systems achieved dynamic compositional stability about 6 days after inoculation which could be maintained for ≥20 days. In conclusion, the CDFF facilitated the continuous maintenance of bacteriologically stable microcosms that were compositionally similar to ex situ inocula.
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Carneiro, Marcos Tavares, Daniel Vidal Perez, Renato Castiglia Feitosa, and Julio Cesar Wasserman. "Separation of Escherichia coli from natural samples for identification of sources and microcosm inoculation." Brazilian Journal of Microbiology 51, no. 4 (September 12, 2020): 2015–20. http://dx.doi.org/10.1007/s42770-020-00374-2.

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Lee, Jiyeon, Sungbo Cho, and Hee-Eun Kim. "Antimicrobial Effects of Non-Thermal Atmospheric Pressure Plasma on Oral Microcosm Biofilms." International Journal of Environmental Research and Public Health 20, no. 3 (January 30, 2023): 2447. http://dx.doi.org/10.3390/ijerph20032447.

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We comparatively evaluated the antibacterial effects of non-thermal atmospheric pressure plasma (NTAPP) on oral microcosm biofilms. Oral microcosm biofilms, which are derived from inoculation with human saliva, were cultured on 48 hydroxyapatite disks for 6 days. The prepared biofilms were divided into three different daily treatment groups: distilled water for 1 min, 0.12% chlorhexidine (CHX) for 1 min, and NTAPP for 5 min. Using a quantitative light-induced fluorescence-digital camera, the red fluorescence intensity of the biofilms was measured as red/green ratios (RatioR/G) before and after treatment. Total and aciduric bacteria were counted as colony-forming units. Using live/dead bacterial staining, bacterial viability was calculated as the RatioG/G+R. RatioR/G was approximately 0.91-fold lower in the NTAPP group than in the CHX group on day 1 of treatment (p = 0.001), and approximately 0.94-fold lower on both days 2 and 3 (p < 0.001). The number of total bacteria was higher in the NTAPP group than in the CHX group, but not significantly different. The number of aciduric bacteria was lowest in the CHX group (p < 0.001). However, bacterial viability was lowest in the NTAPP group. Restricted bacterial aggregation was observed in the NTAPP group. These findings suggest that NTAPP may more effectively reduce the pathogenicity of oral microcosm biofilms than 0.12% CHX.
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Sudirman, Sudirman. "EFFECT OF NEMATODE POPULATION DENSITIES ON TRAPPING ACTIVITY OF NEMATOPHAGOUS FUNGUS Arthrobotrys dactyloides ON Meloidogyne javanica." Jurnal Hama dan Penyakit Tumbuhan Tropika 11, no. 1 (February 5, 2011): 78–86. http://dx.doi.org/10.23960/j.hptt.11178-86.

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Trapping activities of some nematophagous fungi were suggested to be related to the population density of nematodes. This study determined the trapping activity of Arthrobotrys dactyloides due to the effects of (i) different populations of Meloidogyne javanica, (ii) the presence of Caenorhabditis elegans, (iii) repeated inoculation of M. javanica, and (iv) different populations of both C. elegans and M. javanica. Experiments were conducted using a “standard slide test” and “soil microcosm” inoculated with A. dactyloides formulated in kaolin-alginate granules and with low nematode populations ranged from 6 to 14 juveniles per g soil. Results showed that ring formation and trapping activity of A. dactyloides increased with the increase of M. javanica population. The presence of C. elegans increased M. javanica mortality. Repeated inoculation of M. javanica maintained trapping activity of A. dactyloides. The mortality of M. javanica increased with the increase of both C. elegans and M. javanica population.
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Underthun, Kristina, Jaysankar De, Alan Gutierrez, Rachael Silverberg, and Keith R. Schneider. "Survival of Salmonella and Escherichia coli in Two Different Soil Types at Various Moisture Levels and Temperatures." Journal of Food Protection 81, no. 1 (December 28, 2017): 150–57. http://dx.doi.org/10.4315/0362-028x.jfp-17-226.

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ABSTRACT With the increased consumption of fresh produce, a proportional increase in numbers of produce-related foodborne illness has been observed. An estimate of foodborne illness during 1998 to 2008 attributed ∼46% of the incidences to produce. Any foodborne illness associated with produce can have devastating consequences to the industry. The most recent data from the Centers for Disease Control and Prevention implicate leafy vegetables, vine-stalk vegetables, root vegetables, and sprouts as the most common cause of produce-related foodborne outbreaks. Excess rainfall or flooding, mainly by altering levels of soil moisture and oxygen content, affects the microbial community in soil. The goal of this research was to determine the survivability of a three-serovar Escherichia coli and a five-serovar Salmonella enterica cocktail in microcosms prepared with Candler sand (CS) and Orangeburg sandy loam (OSL) soils. Microcosms were prepared with low, medium, and high volumetric water contents and were incubated at 20 and 30°C. Serotyping was used to determine which E. coli or Salmonella serovar(s) from each cocktail persisted. Microcosm inoculation levels were ∼7.0 log CFU/g. Sampling for CS and OSL microcosms incubated at 20°C ended on day 364 and 357, respectively. The reduction of Salmonella and E. coli to below the limit of detection (extinction) in CS microcosms (incubated at 30°C at all volumetric water content [VWC] levels) was reached on day 168 and 56, respectively. Extinction of Salmonella and E. coli in OSL microcosms (incubated at 30°C at all VWCs) was reached on day 168 and 224, respectively. Of the Salmonella and E. coli serovars analyzed, Salmonella Javiana persisted the longest in both soil types, whereas E. coli O104:H4 and E. coli O145 persisted the longest in CS and OSL microcosms, respectively. Results from the current study suggest that soil type and temperature influenced pathogen persistence in CS and OSL soils more than moisture level and pathogen type.
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Bodor, Attila, Péter Petrovszki, Ágnes Erdeiné Kis, György Erik Vincze, Krisztián Laczi, Naila Bounedjoum, Árpád Szilágyi, et al. "Intensification of Ex Situ Bioremediation of Soils Polluted with Used Lubricant Oils: A Comparison of Biostimulation and Bioaugmentation with a Special Focus on the Type and Size of the Inoculum." International Journal of Environmental Research and Public Health 17, no. 11 (June 9, 2020): 4106. http://dx.doi.org/10.3390/ijerph17114106.

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Used lubricant oils (ULOs) strongly bind to soil particles and cause persistent pollution. In this study, soil microcosm experiments were conducted to model the ex situ bioremediation of a long term ULO-polluted area. Biostimulation and various inoculation levels of bioaugmentation were applied to determine the efficacy of total petrol hydrocarbon (TPH) removal. ULO-contaminated soil microcosms were monitored for microbial respiration, colony-forming units (CFUs) and TPH bioconversion. Biostimulation with inorganic nutrients was responsible for 22% of ULO removal after 40 days. Bioaugmentation using two hydrocarbon-degrader strains: Rhodococcus quingshengii KAG C and Rhodococcus erythropolis PR4 at a small inoculum size (107 CFUs g−1 soil), reduced initial TPH concentration by 24% and 29%, respectively; the application of a higher inoculum size (109 CFUs g−1 soil) led to 41% and 32% bioconversion, respectively. After 20 days, all augmented CFUs decreased to the same level as measured in the biostimulated cases, substantiating the challenge for the newly introduced hydrocarbon-degrading strains to cope with environmental stressors. Our results not only highlight that an increased number of degrader cells does not always correlate with enhanced TPH bioconversion, but they also indicate that biostimulation might be an economical solution to promote ULO biodegradation in long term contaminated soils.
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Ingrid, Lenoir, Anissa Lounès-Hadj Sahraoui, Laruelle Frédéric, Dalpé Yolande, and Fontaine Joël. "Arbuscular mycorrhizal wheat inoculation promotes alkane and polycyclic aromatic hydrocarbon biodegradation: Microcosm experiment on aged-contaminated soil." Environmental Pollution 213 (June 2016): 549–60. http://dx.doi.org/10.1016/j.envpol.2016.02.056.

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Edge, Thomas A., and R. Campbell Wyndham. "Predicting survival of a genetically engineered microorganism,Pseudomonas chlororaphis3732RN-L11, in soil and wheat rhizosphere across Canada with linear multiple regression models." Canadian Journal of Microbiology 48, no. 8 (August 1, 2002): 717–27. http://dx.doi.org/10.1139/w02-066.

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Pseudomonas chlororaphis 3732RN-L11 survival rates in soil and wheat rhizosphere were measured using intact soil core microcosms representing 23 sites across Canada. Linear multiple regression (LMR) models were developed to predict the survival rate of this genetically engineered microorganism (GEM) as a function of soil parameters measured at the time of microcosm inoculation. LMR models were tested by comparing their predicted survival rates with observed survival rates from environmental introductions of the GEM by Gagliardi et al. (2001) at five field sites across Canada over two years. No soil parameter (e.g., % clay) was highly correlated with GEM survival rates in soil or wheat rhizosphere. Total fungal colony-forming units (CFUs), % soil titanium (positive correlations), and % soil magnesium (negative correlation) were found to be the best LMR predictors of GEM survival rates in soil over two years. Total soil bacterial CFUs, nitrate, % soil potassium (positive correlations), and exchangeable magnesium (negative correlation) were found to be the best LMR predictors of GEM survival rate in wheat rhizosphere over two years. While LMR models were statistically significant, they were unable to reliably predict the survival rate of the GEM in field trial introductions. The results indicate that there can be considerable uncertainty associated with predicting GEM survival for multi-site environmental introductions.Key words: Pseudomonas chlororaphis 3732RN-L11, survival, soil, Canada.
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Dissertations / Theses on the topic "Microcosm Inoculation"

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Mugnai, Gianmarco. "DEVELOPMENT OF INDUCED BIOLOGICAL SOIL CRUSTS AND THEIR EFFECT ON SOIL PROPERTIES." Doctoral thesis, 2018. http://hdl.handle.net/2158/1145692.

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Book chapters on the topic "Microcosm Inoculation"

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Gonçalves, João, Bruno Marcos, Márcia Bessa da Silva, Inês Conceição, João Pissarra, Jéssica Roque, Paula Tamagnini, Paula Melo, Ruth Pereira, and João Honrado. "Spectral monitoring of a system for the rehabilitation of burned soils based on inoculation with cyanobacteria and microalgae." In Advances in Forest Fire Research 2022, 381–84. Imprensa da Universidade de Coimbra, 2022. http://dx.doi.org/10.14195/978-989-26-2298-9_60.

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Inocula containing photosynthetic microorganisms such as cyanobacteria and microalgae can promote the formation of biocrusts, improving the soil properties and allowing ecosystem recovery. The GreenRehab project aims to develop a low-cost, eco-friendly, and easy-to-implement system to rehabilitate burned soils and protocols to evaluate the success of an ecosystem after-fire recovery. For this purpose, native cyanobacteria and microalgae were isolated from soil/biocrusts and tested, with selected strains being cultivated on a large scale. To evaluate the performance of the proposed rehabilitation system (based on the inoculation of native cyanobacteria and microalgae), we analyzed spectral data scanned from a portable visible/near-infrared spectrometer that indirectly estimates top-soil photosynthetic activity. Several spectral indices based on the normalized difference combination of spectral bands were calculated and compared for their ability to assess photosynthetic activity over time. Results showed that treatments with microalgae and cyanobacteria effectively enhanced photosynthetic activity, with Trichocoleus stimulating soil rehabilitation the most. Moreover, the best performing normalized difference index was the one combining the 660 nm and 860 nm wavelengths. Parallelly, very-high resolution imagery obtained from a UAV equipped with a multispectral camera is currently being tested to assess the performance of different inocula previously selected from microcosm experiments in environmental conditions closer to real ones found in post-fire scenarios. Preliminary results led us to develop an integrated protocol for image acquisition, radiometric calibration and photogrammetric post-processing. This allowed us to characterize baseline conditions in the test area, including geomorphology, vegetation and portray fine-scale patterns in greenness and photosynthetic activity. Overall, spectral measurements and indices from portable spectrometers and UAVs open the possibility of assessing which treatments exploited in the GreenRehab project effectively enhance soil rehabilitation and recovery from frequent fires. Such innovation may translate into other 'real-world' applications in ecology, forestry, and agronomy fields.
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