Relevant bibliographies by topics / Microbiology diagnostics

Academic literature on the topic 'Microbiology diagnostics'

Author: Grafiati
Published: 10 December 2022
Last updated: 29 January 2023

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Journal articles on the topic "Microbiology diagnostics"

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Hawkey, Peter M. "Molecular diagnostics in clinical microbiology." Journal of Infection 40, no. 2 (March 2000): A8—A9. http://dx.doi.org/10.1016/s0163-4453(00)80032-8.

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Patel, Robin, and Brad S. Karon. "Advances Afoot in Microbiology." Journal of Clinical Microbiology 55, no. 7 (May 24, 2017): 1984–88. http://dx.doi.org/10.1128/jcm.00664-17.

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ABSTRACT In 2016, the American Academy of Microbiology convened a colloquium to examine point-of-care (POC) microbiology testing and to evaluate its effects on clinical microbiology. Colloquium participants included representatives from clinical microbiology laboratories, industry, and the government, who together made recommendations regarding the implementation, oversight, and evaluation of POC microbiology testing. The colloquium report is timely and well written (V. Dolen et al., Changing Diagnostic Paradigms for Microbiology , 2017, https://www.asm.org/index.php/colloquium-reports/item/6421-changing-diagnostic-paradigms-for-microbiology?utm_source=Commentary&utm_medium=referral&utm_campaign=diagnostics ). Emerging POC microbiology tests, especially nucleic acid amplification tests, have the potential to advance medical care.
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Obranic, Sonja. "Molecular diagnostics in the clinical microbiology laboratory." Molecular and experimental biology in medicine 2, no. 2 (October 5, 2019): 1–8. http://dx.doi.org/10.33602/mebm.2.2.1.

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Molecular diagnostics is broadly available in clinical microbiology laboratories worldwide, especially for the detection and identification of difficult-to-cultivate microorganisms. The field of clinical microbiology has experienced significant changes over the past decade due to extensive molecular biology research that resulted in novel molecular diagnostics technologies. These new technologies are being introduced in clinical microbiology laboratories with the aim of improving sensitivity, specificity, accuracy and time-to-diagnosis, ensuring valuable data for effective infectious disease clinical management, infection control and surveillance. They have a potential to greatly improve general healthcare, but also present certain challenges, mainly regarding the cost and the proper definition of test ordering and interpretation. This review will discuss the current and potential application of next-generation sequencing, digital PCR and syndromic multiplex molecular assays in clinical microbiology.
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Moser, Claus, Trine Rolighed Thomsen, and Niels Høiby. "Next generation microbiology and cystic fibrosis diagnostics." Current Opinion in Pulmonary Medicine 24, no. 6 (November 2018): 599–605. http://dx.doi.org/10.1097/mcp.0000000000000516.

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Taravati, Parisa, Deborah Lam, and Russell N. Van Gelder. "Role of Molecular Diagnostics in Ocular Microbiology." Current Ophthalmology Reports 1, no. 4 (September 28, 2013): 181–89. http://dx.doi.org/10.1007/s40135-013-0025-1.

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Westblade, Lars F., Alex van Belkum, Adam Grundhoff, George M. Weinstock, Eric G. Pamer, Mark J. Pallen, and W. Michael Dunne. "Role of Clinicogenomics in Infectious Disease Diagnostics and Public Health Microbiology." Journal of Clinical Microbiology 54, no. 7 (February 24, 2016): 1686–93. http://dx.doi.org/10.1128/jcm.02664-15.

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Clinicogenomics is the exploitation of genome sequence data for diagnostic, therapeutic, and public health purposes. Central to this field is the high-throughput DNA sequencing of genomes and metagenomes. The role of clinicogenomics in infectious disease diagnostics and public health microbiology was the topic of discussion during a recent symposium (session 161) presented at the 115th general meeting of the American Society for Microbiology that was held in New Orleans, LA. What follows is a collection of the most salient and promising aspects from each presentation at the symposium.
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Kociolek, Larry K. "Strategies for Optimizing the Diagnostic Predictive Value of Clostridium difficile Molecular Diagnostics." Journal of Clinical Microbiology 55, no. 5 (March 8, 2017): 1244–48. http://dx.doi.org/10.1128/jcm.00147-17.

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ABSTRACT Because nucleic acid amplification tests (NAATs) do not distinguish Clostridium difficile infection (CDI) and asymptomatic C. difficile carriage, the diagnostic predictive value of NAATs is limited when used in patients with a low probability of CDI. In this issue of the Journal of Clinical Microbiology , Truong et al. (J. Clin. Microbiol., 55:1276–1284, 2017, https://doi.org/10.1128/JCM.02319-16 ) report significant reductions in hospital-onset CDI and oral vancomycin utilization at their institution following implementation of a novel intervention that leveraged their clinical bioinformatics resources to prevent C. difficile testing of stools from patients without clinically significant diarrhea and in patients with recent laxative use.
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Paramasivam, Saravanan, and Satish Kumar. "Diagnostic and immunoprophylactic applications of synthetic peptides in veterinary microbiology." Microbiology Research 1, no. 1 (October 26, 2009): 1. http://dx.doi.org/10.4081/mr.2010.e1.

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Chemically synthesized peptides are considered as potential reagents for various applications in biological sciences. They mimic naturally occurring peptides or segments of proteins and have emerged as diagnostic reagents and safe immunogens in animal science. Carefully selected peptides resembling authentic epitopes serve as synthetic antigens in diagnostic tests. Synthetic peptide-based vaccines can elicit antibodies against animal pathogens. The early use of synthetic peptides as a vaccine for foot-and-mouth disease stimulated interest in the development of peptide-based diagnostics and immunoprophylactics. The development of a peptide vaccine for canine parvovirus confirmed the usefulness of peptides as immunoprophylactics. Recently, the advent of the technology for the development of multiple antigenic peptides (MAPs) has provided a well-defined method for the production of highly immunogenic peptides and anti-peptide antibodies. Antibodies raised against major epitopes can be used in the detection of the native antigen (virus) in the enzyme-linked immunosorbent assay (ELISA) and other tests, vindicating the usefulness of peptides for safe, chemically defined, non-infectious diagnostics and immunoprophylactics. This article focuses on the methods for selecting and preparing peptides for the predicted epitopes, their characterization and use, and the application of MAPs.
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Lopez-Siles, Mireia, Sylvia H. Duncan, L. Jesús Garcia-Gil, and Margarita Martinez-Medina. "Faecalibacterium prausnitzii: from microbiology to diagnostics and prognostics." ISME Journal 11, no. 4 (January 3, 2017): 841–52. http://dx.doi.org/10.1038/ismej.2016.176.

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Yagci, Aysegul Karahasan. "Future trends of molecular diagnostics in clinical microbiology." Current Opinion in Biotechnology 22 (September 2011): S29. http://dx.doi.org/10.1016/j.copbio.2011.05.057.

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Dissertations / Theses on the topic "Microbiology diagnostics"

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Spichler, Anne, Bonnie L. Hurwitz, David G. Armstrong, and Benjamin A. Lipsky. "Microbiology of diabetic foot infections: from Louis Pasteur to 'crime scene investigation'." BioMed Central Ltd, 2015. http://hdl.handle.net/10150/610294.

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Were he alive today, would Louis Pasteur still champion culture methods he pioneered over 150 years ago for identifying bacterial pathogens? Or, might he suggest that new molecular techniques may prove a better way forward for quickly detecting the true microbial diversity of wounds? As modern clinicians faced with treating complex patients with diabetic foot infections (DFI), should we still request venerated and familiar culture and sensitivity methods, or is it time to ask for newer molecular tests, such as 16S rRNA gene sequencing? Or, are molecular techniques as yet too experimental, non-specific and expensive for current clinical use? While molecular techniques help us to identify more microorganisms from a DFI, can they tell us ‘who done it?', that is, which are the causative pathogens and which are merely colonizers? Furthermore, can molecular techniques provide clinically relevant, rapid information on the virulence of wound isolates and their antibiotic sensitivities? We herein review current knowledge on the microbiology of DFI, from standard culture methods to the current era of rapid and comprehensive ‘crime scene investigation' (CSI) techniques.
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Giordani, Federica. "New approaches to fluorescence-based diagnostics for human African trypanosomiasis." Thesis, University of Glasgow, 2011. http://theses.gla.ac.uk/2454/.

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In the absence of any vaccine, prophylactic drug and effective vector control, the fight against human African trypanosomiais (HAT) is based on the the combination of active case-finding and consequent drug treatment of identified positive cases. Unfortunately, low sensitivity and specificity of current diagnostic techniques often result in misdiagnosis, leaving infected patients without cure or exposing them to inappropriate chemotherapy protocols, which use dangerous and expensive drugs. The development of more efficient, simple, cheap and field-robust diagnostic tests is, therefore, urgently needed. In the field, direct observation by light microscopy of trypanosomes in human fluids (blood, lymph node aspirate, cerebrospinal fluid) is considered the ideal way of confirming HAT infection. However, in practice this approach is problematic, especially for the Gambian form of the disease, where patients may present with very low parasitaemia. Detection limits of parasitological techniques can be improved by adding a preliminary step of sample concentration, although this further increases the laboriousness of HAT diagnostic algorithm. Recent advances in fluorescence microscopy could be exploited to facilitate trypanosome detection. The introduction and implementation of fluorescence microscopy in HAT endemic countries would offer the advantages of an increased overall sensitivity of microscopical examination and a more rapid screening of the specimen. In contrast to traditional, expensive and fragile fluorescence microscopes, new LED-illuminated instruments are relatively cheap, very efficient and portable, lending themselves to utilisation in poorly equipped rural settings. In order to design a new diagnostic tool that exploits LED technology, however, selective and reliable fluorescent markers to label trypanosomes in human fluids are needed. The development of new tools to assist in the diagnosis of African trypanosomiasis by use of LED fluorescence microscopy was the overall objective of this project. The work was mainly focused on testing various fluorescent compounds for their ability to selectively stain trypanosomes. Fluorophores were otained from commercial and academic sources, or else directly synthesised during the project. An important requirement evaluated was the compounds’ compatibility with the currently available SMR LED Cytoscience fluorescence microscope, developed and kindly provided by our collaborator Prof. D. Jones (Philipps University, Marburg). The utility of a UV LED-driven microscope in performing the arsenical drug resistance test was also assessed. This assay, developed in our laboratory to detect trypanosome strains resistant to arsenical and diamidine compounds, could represent a useful tool for chemotherapeutic decision making in the field, where resistance to arsenical drugs is a rising problem.
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Yngve, Sara. "Optimization of PCR Sensitivity for Detection of Bacterial Species in Blood of Patients with Suspected Sepsis." Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-12246.

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Sepsis is commonly caused by bacteria, fungi or both present in the blood stream during inflammation. In response, inflammatory cascades are released in multiple organ systems which if prolonged causes sepsis and can eventually lead to organ failure and death. The major diagnostic technique of sepsis is blood culturing. However, the technique is time consuming and lacks sensitivity; especially in patients under antimicrobial therapy. Molecular techniques particularly PCR could possibly become implemented in sepsis diagnostics in the future. The aim of the thesis was to compare the effect on PCR sensitivity by different PCR kits, with optimized PCR conditions to find an ideal Real-time PCR applicable for direct detection of rRNA or rDNA in whole blood, using the 16S rDNA gene. The study also surveyed the overall background flora of bacterial species circulating in the blood. During the optimization Haemophillus influenzae and Streptococcus pneumoniae were added to whole blood, rRNA or rDNA was isolated and extracted and subsequently processed by Real-time PCR. Four commercially available PCR kits were compared. Attempts using rRNA did not significantly increase the PCR sensitivity. LightCycler FastStart DNA Master SYBR Green I kit (Roche Diagnostics) used for rDNA, generated low cp-values, the cleanest sequences and the finest separation between amplification curves. Twenty whole blood and pre-cultured patient samples were processed by the optimized PCR. The effect on PCR sensitivity by pre-culturing patient blood samples was studied and no statistical difference was noted. Increased PCR sensitivity is essential for implementation of PCR techniques in sepsis diagnostics.
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Näslund, Jonas. "Rift Valley fever : development of diagnostics and vaccines." Doctoral thesis, Umeå universitet, Klinisk mikrobiologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-30676.

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Rift Valley Fever virus (RVFV) causes an infection with severe impact on animal and human health. The disease is endemic throughout almost the entire African continent and large regions of the Arabian Peninsula. During epidemics, high mortality is observed in animals, especially among cattle, goats, and sheep. In humans, the symptoms vary from a benign influenza-like disease to a life-threatening hemorrhagic fever. Due to the devastating effect on communities in endemic regions and the possibility of further spread of this virus, there is an imperative need to improve and develop control measurements against this emerging disease. Therefore, this thesis focuses on diagnostics and vaccines against RVFV. RVFV infection kinetics was studied in a mouse model system by detection and quantification of viral genomes, using a developed quantitative real-time PCR (QRT-PCR) method. This novel QRT-PCR method proved to be reliable and serves as a supplement to standard diagnostics, direct virus isolation and serological methods. High levels of viral RNA were found in blood and liver samples from experimentally infected mice during the first days post infection. Thereafter the levels declined rapidly and dropped below detection limit approximately seven days post infection. The QRT-PCR technique was also used in a study aimed to improve diagnosis of RVFV from field samples collected on filter strips. Today, the available RVFV vaccines are only approved for animal use and these vaccines have several shortcomings. Since RVFV is a highly pathogenic organism requiring bio-safety level 3 laboratories, two different none-replicating vaccine approaches have been applied and evaluated using a mouse model. A DNA based vaccine, administered via gene-gun, and the use of virus-like particles (VLP), by the intra-peritoneal route. RVFV specific and neutralising antibodies were raised with both vaccine approaches. However, VLP vaccination against Rift valley Fever proved to be more promising as a future vaccine, since higher titres of neutralising antibodies and improved survival rate were found upon a lethal RVFV challenge in mice. In conclusion, a sensitive and specific method for quantifying RVFV infection and a promising vaccine candidate against RVFV were developed.
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Bourquin, Yannyk Parulian Julian. "Shaping surface waves for diagnostics." Thesis, University of Glasgow, 2012. http://theses.gla.ac.uk/4167/.

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Infectious diseases continue to kill millions of people every year and are a significant burden on the socio-economic development of developing countries. After many years of international policy aimed at containing diseases, it has recently become an explicit aim to move towards elimination of infectious diseases. However, if this is to occur, it will be necessary to have highly eficacious diagnostic tools to ensure infected individuals are identified and treated. However, the diagnosis of infectious diseases in the developed and developing world requires the full integration of complex assays in easy-to-use platforms with robust analytical performances at low cost. Many relevant bioanalytical technologies have been developed for use in laboratories and clinics, including the current gold standard for the diagnosis of tuberculosis and malaria. The miniaturization and integration of complex functions into lab-on-a-chip (LOC)technologies using microfluidics have only had limited success in translating diagnosis assays out of a centralized laboratory to point-of-care (POC) settings, because they still remain constrained due to chip interconnection and they are either not likely to go out of research laboratories or are not appropriate for low resource settings. In this thesis, a new microfluidic platform was developed that reduced the dependency of the diagnostic procedure on large laboratory instruments providing simplicity of use, enabling the patient sample to be processed and diagnosed on a low cost, disposable biochip. Surface acoustic wave (SAW) devices, which are commonly used in mobile phone technologies, were adapted to provide controlled microfluidic functions by shaping the SAW using particular designs of electrodes and phononic structures. The control of lateral positioning of the SAW was demonstrated using a slanted finger interdigitated transducer (IDT) in a frequency tuneable manner allowing microfluidic functions such as mixing, moving and merging, sequentially performed using a single IDT both on the substrate and on a disposable chip. Alternatively, phononic bandgaps were designed to break the symmetry of the SAW in a tuneable manner and gradient index phononic crystals (GRIN-PC) lenses were designed to focus the SAW and successfully increased the amplitude of the wave by a factor 3 while the focal position could be tuned with the frequency. The potential of these techniques was demonstrated by controlling the amplitude and direction of water jet towers by the use of a phononic horn structure that allowed the enhancement of energy at defined positions and by propelling and directing a macrometer scale object in water using a slanted IDT. As proof of concepts of diagnostic devices for the developing world, an immunoassay for tuberculosis using only mobile phone technologies (SAW, light-emitting diode(LED) and complementary metaloxidesemiconductor (CMOS) camera) was demonstrated with a limit of detection of 1 pM, which is the limit required in an interferon-release assay. This limit of detection was only achievable because of the ability of SAW to increase the mixing and to reduce the non-specific binding. Furthermore, a method to enrich malaria infected cells, based on SAW and isopycnic gradient, was also demonstrated and showed an enrichment up to 100x in the equivalent of a fingerprick of blood in 3 seconds. This technique will allow to reduce the limit of detection of the current gold standard. This platform not only opens a clear road toward POC diagnostics due to its size, cost, versatility and ease in integration, but has also the potential to provide useful tools in laboratory settings for large scale, high throughput technologies.
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Vervier, Kevin. "Méthodes d’apprentissage structuré pour la microbiologie : spectrométrie de masse et séquençage haut-débit." Thesis, Paris, ENMP, 2015. http://www.theses.fr/2015ENMP0081/document.

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L'utilisation des technologies haut débit est en train de changer aussi bien les pratiques que le paysage scientifique en microbiologie. D'une part la spectrométrie de masse a d'ores et déjà fait son entrée avec succès dans les laboratoires de microbiologie clinique. D'autre part, l'avancée spectaculaire des technologies de séquençage au cours des dix dernières années permet désormais à moindre coût et dans un temps raisonnable de caractériser la diversité microbienne au sein d'échantillons cliniques complexes. Aussi ces deux technologies sont pressenties comme les piliers de futures solutions de diagnostic. L'objectif de cette thèse est de développer des méthodes d'apprentissage statistique innovantes et versatiles pour exploiter les données fournies par ces technologies haut-débit dans le domaine du diagnostic in vitro en microbiologie. Le domaine de l'apprentissage statistique fait partie intégrante des problématiques mentionnées ci-dessus, au travers notamment des questions de classification d'un spectre de masse ou d'un “read” de séquençage haut-débit dans une taxonomie bactérienne.Sur le plan méthodologique, ces données nécessitent des développements spécifiques afin de tirer au mieux avantage de leur structuration inhérente: une structuration en “entrée” lorsque l'on réalise une prédiction à partir d'un “read” de séquençage caractérisé par sa composition en nucléotides, et un structuration en “sortie” lorsque l'on veut associer un spectre de masse ou d'un “read” de séquençage à une structure hiérarchique de taxonomie bactérienne
Using high-throughput technologies is changing scientific practices and landscape in microbiology. On one hand, mass spectrometry is already used in clinical microbiology laboratories. On the other hand, the last ten years dramatic progress in sequencing technologies allows cheap and fast characterization of microbial diversity in complex clinical samples. Consequently, the two technologies are approached in future diagnostics solutions. This thesis aims to play a part in new in vitro diagnostics (IVD) systems based on high-throughput technologies, like mass spectrometry or next generation sequencing, and their applications in microbiology.Because of the volume of data generated by these new technologies and the complexity of measured parameters, we develop innovative and versatile statistical learning methods for applications in IVD and microbiology. Statistical learning field is well-suited for tasks relying on high-dimensional raw data that can hardly be used by medical experts, like mass-spectrum classification or affecting a sequencing read to the right organism. Here, we propose to use additional known structures in order to improve quality of the answer. For instance, we convert a sequencing read (raw data) into a vector in a nucleotide composition space and use it as a structuredinput for machine learning approaches. We also add prior information related to the hierarchical structure that organizes the reachable micro-organisms (structured output)
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Oliver, Lee D. Jr. "Development of Novel Chimeric Epitope Based Diagnostic Antigens and Vaccines for Lyme Disease." VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/3876.

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Lyme disease (LD) is the leading arthropod-borne disease in North America with 300-600,000 cases each year. There are currently no approved human LD vaccines. Outer surface protein C (OspC) has emerged as a leading vaccine candidate and an attractive diagnostic marker due to its antigenicity and expression early in infection. Several chimeric, epitope based OspC derived proteins were generated. The constructs were found to be highly immunogenic in mice and vaccination induced complement-dependent bactericidal antibodies. These results suggest that a broadly protective polyvalent OspC epitope based vaccine can be produced. Currently, LD diagnostic approaches are unreliable and unable to differentiate between early and late stage disease. An Ab response to OspE family proteins occurs later in infection. The two-Ag diagnostic assay using chimeric OspC proteins and a site-directed mutant of an OspE paralog, accurately differentiated between early and late infection in experimentally infected canines and humans.
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López, Siles Mireia. "Ecophysiology and philogeny of Faecalibacterium prausnitzii in healthy and diseased gut. Application in Inflamatory Bowel Disease diagnostics." Doctoral thesis, Universitat de Girona, 2016. http://hdl.handle.net/10803/369044.

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In this PhD thesis Faecalibacterium prausnitzii populations of patients with gut disease and healthy individuals have been characterized. First, isolates from healthy volunteers have been phenotypically characterised, which has allowed to gain insight into the physiology of this species. A possible link between F. prausnitzii sensitivity to changes in gut physicochemical conditions and its disappearance in a diseased gut has been revealed. Second, molecular studies on F. prausnitzii populations have allowed to define two phylogroups within this species, and to describe the diversity of phylotypes in healthy individuals and in patients with intestinal disease. The phylotypes specifically compromised in patients suffering some gut disorders have been identified. Finally, new molecular tools for the detection and quantification of this species and its phylogroups have been designed. Their usefulness to be implemented as complementary molecular tools for the diagnosis and prognosis of intestinal diseases has been determined.
En aquesta tesi doctoral s'ha estudiat la població de Faecalibacterium prausnitzii de pacients amb malalties intestinals i individus sans. En primer lloc, es va realitzar una caracterització fenotípica d'aíllats d'aqueta espècie obtinguts d'individus sans, el que ha permès adquirir coneixement sobre la fisiologia d'aquesta espècie. S'ha evidenciat una possible relació entre la sensibilitat de F.prausnitzii a canvis en les condicions fisicoquímiques de l'intestí i la seva desaparició en un intestí malalt. En segon lloc, s'han realitzat estudis moleculars de les poblacions de F. prausnitzii. Això ha permès definir dos filogrups dins d'aquesta espècie, i descriure la diversitat de filotips en individus sans i pacients amb malalties intestinals.Per primera vegada, s'han identificat els filotips especificament compromesos en pacients que pateixen determinades malalties intestinals. Per últim, s’han dissenyat eines moleculars per a la detecció i quantificació d'aquesta espècie i els seus filogrups. S’ha determinat la utilitat d’aquestes eines moleculars per al suport al diagnòstic o prognòstic de malalties intestinals.
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Wrightson, John M. "Pathogen identification in lower respiratory tract infection." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:30c757ec-99b7-492e-a12e-ff996581863a.

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Treatment of lower respiratory tract infection (pneumonia and pleural infection) relies on the use of empirical broad spectrum antibiotics, primarily because reliable pathogen identification occurs infrequently. Another consequence of poor rates of pathogen identification is that our understanding of the microbiology of these infections is incomplete. This thesis addresses some of these issues by combining the acquisition of high quality lower respiratory tract samples, free from nasooropharyngeal contamination, with novel molecular microbiological techniques in an attempt to increase rates of pathogen identification. Four main areas are examined: (i) The role of so-called ‘atypical pneumonia’ bacteria in causing pleural infection. These pathogens have been previously identified in the pleural space infrequently and routine culture usually fails to isolate such bacteria. High sensitivity nested polymerase chain reaction (PCR) is a culture-independent technique which is used to undertake a systematic evaluation for these pathogens in pleural infection samples. (ii) The role of Pneumocystis jirovecii in pleural infection, either as a co-infecting pathogen or in monomicrobial infection. This fungus causes severe pneumonia, particularly in the immunosuppressed, but is increasingly recognised as a co-pathogen in community-acquired pneumonia, and is frequently isolated in the upper and lower respiratory tract in health. A high sensitivity real-time PCR assay is used to examine for this fungus. (iii) Ultra-deep sequencing of the 16S rRNA gene is used to perform a comprehensive microbial survey in samples taken from the multi-centre MIST2 study of pleural infection. The techniques employed allow analysis of polymicrobial samples and give very high taxonomic resolution, whilst incorporating methods to control for potential contamination. Further, these techniques provide confirmation of the results from the ‘atypical’ bacteria nested PCR study. (iv) Bedside ultrasound-guided percutaneous transthoracic needle aspiration (TNA) of consolidated lung is undertaken in patients with pneumonia, as part of the PIPAP study. An evaluation is undertaken of the efficacy and acceptability of TNA. Aspirate samples acquired are also processed using ultra-deep sequencing of the 16S rRNA gene. Other samples obtained as part of the PIPAP study, such as ‘control’ lung aspirates and ‘control’ pleural fluid samples, are similarly processed to enable calculation of sensitivity and specificity of the sequencing methodology.
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Schuurman, Timothy. "Developments and clinical applications in diagnostic molecular microbiology." [S.l.] : Rotterdam : [The Author] ; Erasmus University [Host], 2008. http://hdl.handle.net/1765/13137.

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Books on the topic "Microbiology diagnostics"

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Bone and joint infections: From microbiology to diagnostics and treatment. Hoboken, NJ: Wiley Blackwell, 2015.

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Moran-Gilad, Jacob, and Yael Yagel, eds. Application and Integration of Omics-powered Diagnostics in Clinical and Public Health Microbiology. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-62155-1.

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Schwerdtle, Cornelia. Introduction into darkfield diagnostics: The examination of the native blood according to Prof. Dr. Gunther Enderlein. Hoya, Germany: Semmelweis-Verlag, 1993.

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F, Brooks George, and Jawetz Ernest, eds. Jawetz, Melnick & Adelberg's medical microbiology. 2nd ed. New York: Lange Medical Books/McGraw-Hill, Medical Pub. Division, 2007.

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F, Sahm Daniel, Weissfeld Alice S, and Baron Ellen Jo, eds. Bailey & Scott's diagnostic microbiology. St. Louis: Mosby, 1998.

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F, Sahm Daniel, Weissfeld Alice S, and Bailey W. Robert 1917-, eds. Bailey & Scott's diagnostic microbiology. St. Louis, Mo: Elsevier Mosby, 2007.

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F, Sahm Daniel, and Weissfeld Alice S, eds. Bailey & Scott's diagnostic microbiology. St. Louis: Mosby, 2002.

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R, Mahon Connie, Lehman Donald C, and Manuselis George, eds. Textbook of diagnostic microbiology. 3rd ed. St. Louis, Mo: Saunders Elsevier, 2007.

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1917-, Bailey W. Robert, and Finegold Sydney M. 1921-, eds. Bailey and Scott's diagnostic microbiology. 8th ed. St. Louis: Mosby, 1990.

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Laboratory exercises in diagnostic microbiology. Albany, N.Y: Delmar Publishers, 1996.

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Book chapters on the topic "Microbiology diagnostics"

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Chandler, Darrell P. "Microarray-Based Environmental Diagnostics." In Manual of Environmental Microbiology, 2.3.3–1–2.3.3–13. Washington, DC, USA: ASM Press, 2015. http://dx.doi.org/10.1128/9781555818821.ch2.3.3.

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Zinserling, Vsevolod. "Microbiology and Molecular Biology in Diagnostics." In Infectious Lesions of the Central Nervous System, 1–4. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-96260-9_1.

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Yagel, Yael, and Jacob Moran-Gilad. "Introduction to Advanced Diagnostics in Microbiology." In Application and Integration of Omics-powered Diagnostics in Clinical and Public Health Microbiology, 1–7. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-62155-1_1.

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Lo, Angus C. T., and Kai Man Kam. "Molecular Diagnostics of Sexually Transmitted Diseases." In Advanced Techniques in Diagnostic Microbiology, 535–56. Boston, MA: Springer US, 2012. http://dx.doi.org/10.1007/978-1-4614-3970-7_29.

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Zinserling, Vsevolod. "Microbiology, Molecular Biology, Immunology, and Microscopy in Diagnostics." In Infectious Pathology of the Respiratory Tract, 13–20. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-66325-4_2.

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Saijo, Masayuki. "Recombinant Protein-Based Diagnostics for Viral Hemorrhagic Fevers." In Advanced Techniques in Diagnostic Microbiology, 649–68. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-95111-9_27.

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Hornischer, Klaus, and Susanne Häußler. "Diagnostics and Resistance Profiling of Bacterial Pathogens." In Current Topics in Microbiology and Immunology, 89–102. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/82_2016_494.

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Stallknecht, D. E. "Impediments to Wildlife Disease Surveillance, Research, and Diagnostics." In Current Topics in Microbiology and Immunology, 445–61. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-70962-6_17.

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Muldrew, Kenneth L. "Molecular Diagnostics of Sexually Transmitted Diseases: Bacterial, Trichomonas, and Herpes Simplex Virus Infections." In Advanced Techniques in Diagnostic Microbiology, 67–100. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-95111-9_3.

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Diplock, E. E., H. A. Alhadrami, and G. I. Paton. "Commercial Application of Bioluminescence Full Cell Bioreporters for Environmental Diagnostics." In Handbook of Hydrocarbon and Lipid Microbiology, 4445–58. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-77587-4_347.

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Conference papers on the topic "Microbiology diagnostics"

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Rodríguez-Lázaro, D., and M. Hernández. "Molecular methodology in Food Microbiology diagnostics: trends and current challenges." In 13th World Congress of Food Science & Technology. Les Ulis, France: EDP Sciences, 2006. http://dx.doi.org/10.1051/iufost:20060643.

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carvalho, karen rodrigues vieira, ANA LAURA DE MELO SILVEIRA, JULIA MARIA DOS SANTOS AMARAL, and LEONARDO CAMARGOS SALIBA. "POSSÍVEIS ABORDAGENS DIAGNÓSTICAS FRENTE A PNEUMOCYSTIS JIROVECII." In II Congresso Nacional de Microbiologia Clínica On-line. Revista Multidisciplinar em Saúde, 2022. http://dx.doi.org/10.51161/ii-conamic/6172.

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Introdução: A pneumocystis jirovecii (PDC) é a causa mais prevalente de doença pulmonar oportunista em imunodeprimidos pelo HIV. Devido ao seu tropismo e especificidade para o pulmão humano, ela resulta em pneumonia intersticial grave, com hipoxemia e insuficiência respiratória progressiva. É derivada de um fungo “atípico” por não conter ergosterol na membrana celular, não sendo susceptível à ação da maioria dos antifúngicos, como é o caso da anfotericina B. A probabilidade de PPC está significativamente aumentada quando a contagem de CD4 cai abaixo de 200 células/µL ou é menor que 20% dos linfócitos circulantes. Porém quando se trata do diagnostico, ele depende da obtenção de espécimes respiratórios obtidos de forma invasiva e da visualização do microrganismo mediante técnicas complexas, tornando-se necessário explorar novas abordagens. Objetivo: Abordar possíveis métodos diagnósticos práticos e menos invasivos para detecção da patologia. Metodologia: Realizou-se uma revisão de literatura das bases de dados PubMed e Google Scholar utilizando os descritores: pneumocystis jirovecii, microbiota pulmonar e marcadores diagnósticos de PPC. Foram selecionados artigos publicados a partir de 2008 para base deste estudo. Resultados: O diagnóstico de certeza da patologia depende da identificação do fungo em secreções ou em tecido pulmonar. Devido a pobreza de escarro espontâneo, duas técnicas são realizadas: a indução de escarro e o lavado broncoalveolar. A biópsia transbrônquica e a biópsia por toracotomia são as alternativas secundárias. Para uma abordagem mais rápida e pratica, estuda-se a utilização de amostras biológicas, como a pesquisa de IgA anti-P. jirovecii em saliva. Parece ser promissora, já que os títulos médios de anticorpos IgA detectados na saliva de doentes com infeção foi superior ao detectado nos doentes sem infeção. Outros estudos sugerem que Krebs von den Lungen-6 (KL-6), e S-adenosilmetionina (SAM), poderão ser utilizados como indicadores sensíveis para o diagnóstico da PPC no futuro. Conclusão: Diante de uma suspeita de PPC, o médico se encontra com 2 alternativas: iniciar tratamento de forma empírica ou proceder com uma investigação diagnóstica. Assim, estudos abrangendo métodos mais práticos e rápidos de diagnostico seriam um avanço considerável para os danos causados por métodos invasivos a um paciente que já se encontra imunossuprimido.
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Azevêdo, Sâmya Pires Batista de, Ana Beatriz Silva Barbosa, Raniery Augusto Dos Santos Beserra Nogueira, Thayonara Irineu Da Costa, and Jamile Rodrigues Cosme De Holanda. "UMA REVISÃO DA LITERATURA ACERCA DAS ALTERAÇÕES DA MICROBIOTA VAGINAL NO PERÍODO GESTACIONAL E PARTO." In I Congresso Nacional de Microbiologia Clínica On-Line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/1181.

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Introdução: Durante o período gestacional ocorrem diversas modificações fisiológicas no organismo feminino, e tais modificações são responsáveis por promover alterações no potencial hidrogeniônico (pH) vaginal e no equilíbrio da microbiota local. Nessa fase, as células epiteliais ricas em glicogênio estão abundantes, e como consequência disso vai favorecer a produção de ácido lático pelos lactobacilos, reduzindo ainda mais o pH vaginal. Objetivos: Evidenciar as alterações que ocorrem na microbiota vaginal no decorrer da gestação, e de que forma tais alterações irão influenciar na gravidez e parto. Material e Métodos: O presente estudo consta de uma revisão da literatura, realizada mediante a análise de artigos científicos publicados nas línguas inglesa e portuguesa, entre os anos de 2018 e 2019, por meio de pesquisa nos bancos de dados: Biblioteca Virtual em Saúde. Utilizando-se os Descritores em Ciência da Saúde: Flora; Microorganismos e Vaginose bacteriana, de forma associada com o operador booleano “AND”. Resultados: Observou-se que o pH garante o equilíbrio da flora vaginal, visto que nela há microrganismos residentes que lá vivem de forma comensal, tais como Gardnerella vaginalis, Megasphaera phylotype, espécies de Moliluncus, Bacteroides, Prevotela, Atopobium, e micoplasmas. Durante a gestação, a mulher passa por várias modificações fisiológicas e hormonais que podem vir a alterar, direta ou indiretamente, o pH vaginal e consequentemente proporcionar um bom ambiente para a proliferação desses microrganismos. Como consequência, temos a instalação da vaginose bacteriana, que é uma das infecções do trato genital feminino mais comuns, que atinge, principalmente, as mulheres no período gestacional. Esta pode causar desde uma simples infecção até um parto prematuro ou aborto, a depender da gravidade, diagnóstico e tratamento precoce. Conclusão: É possível constatar que a alteração da flora vaginal da gestante se apresenta de forma muito variada, o que pode dificultar a detecção da vaginose bacteriana. Os estudos comprovaram que gestantes podem apresentar flora vaginal alterada, mesmo sem apresentar sintomatologia. Devido às consequências danosas para a gestante e para o feto, é importante estabelecer rotinas que permitam diagnosticar, esclarecer e intervir nas alterações de flora vaginal no período gestacional.
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"QUALITY OF CLINICAL LABORATORY SERVICES IN A TERTIARY HEALTH CARE FACILITY, IBADAN NORTH LOCAL GOVERNMENT AREA, IBADAN." In International Conference on Public Health and Humanitarian Action. International Federation of Medical Students' Associations - Jordan, 2022. http://dx.doi.org/10.56950/hxts1913.

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Background: Quality clinical laboratory service provision is very important in order to enhance diagnostic value and improve the health status of the community. However, there is very little information on the actual standard adaptation, and implementation, or the impact policy guidelines have had on laboratory services delivery and the community. This study assessed the quality of clinical laboratory services in a tertiary health care facility in Ibadan North Local Government Area, Oyo state. Methods: Interview was conducted for 125 laboratory staff and 426 patients. Five laboratory units were assessed. Data collection was through an observational checklist and semi-structured questionnaires. Observational checklist obtained information on the level of compliance to standard practices and processes. Questionnaires obtained information on laboratory staff socio-demographic characteristics and competency level, and patients’ sociodemographic characteristics and satisfaction with the domains of clinical laboratory services. Descriptive analysis was performed and associations explored between relevant variables using Chi-square test at ‘p’ level of 0.05. Results: Highest level of quality management systems were maintained by the five laboratories while 28.6% had structural deficiencies; 86.9% had compliance with practice quality with Microbiology laboratory unit having highest rating of standard practices (94.6%). Laboratory staff were considered as competent (93.6%) and most are certified by their accreditation body. About 38.6% and 20% attended training in the last 3 months and 6 months respectively. Overall patients’ level of satisfaction was moderate with 53.3% satisfied with the laboratory service received. Half were dissatisfied with the cost of the laboratory tests (49.8%). Patients with higher educational level and income were significantly satisfied than others. Conclusion: Quality of clinical laboratory service delivery in the study setting was good. There is a need for adequate internal and external quality assurance schemes to be in place to constantly monitor the quality of management systems for good service delivery. Key words: Quality, Clinical services, Tertiary healthcare facility, management systems.
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Reports on the topic "Microbiology diagnostics"

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Dolen, Virginia, Kenneth Bahk, Karen C. Carroll, Keith Klugman, and Nathan A. Ledeboer. Changing Diagnostic Paradigms for Microbiology. Chair Melissa B. Miller. American Society for Microbiology, 2017. http://dx.doi.org/10.1128/aamcol.17-18oct.2016.

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