Journal articles: 'Microbiological enhanced oil recovery (MEOR)'

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Brown, Lewis R. "Geomicrobiology." Microbiology Australia 29, no. 1 (2008): 32. http://dx.doi.org/10.1071/ma08032.

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The ever increasing demand for oil, coupled with the reduction in reserves, is increasing emphasis on finding new oil deposits and recovering more oil from known reserves since half to two thirds of all of the oil discovered to date is still in the ground and not recoverable by present technology. There are, however, microbiological techniques that could be of value in extending the time before alternatives to oil as the major energy source are required. Two will be discussed here ? geomicrobiological prospecting and microbial enhanced oil recovery (MEOR).

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Towler, B. F., and B. Bubela. "THE APPLICATION OF MICROBIOLOGICALLY ENHANCED OIL RECOVERY TO THE ALTON FIELD, QUEENSLAND, AUSTRALIA." APPEA Journal 27, no. 1 (1987): 378. http://dx.doi.org/10.1071/aj86033.

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The Alton Field has produced 1.875 million stock tank barrels of oil and is nearing the end of its primary life. It is proposed to enhance the recovery from the field microbiologically. Surfactant producing bacteria will be injected into the reservoir in order to lower the oil/water interfacial tension and mobilise the remaining oil. Laboratory experiments on artifical cores have demonstrated the viability of this process. This MEOR project will initially be done in a one-well cyclic Huff and Puff program.

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Aruawamedor, Onome Christopher, and Sylvester Okotie. "Characterization and Application of Biopolymer Producing Bacteria for Enhanced Oil Recovery." International Journal of Energy and Environmental Research 10, no. 2 (February 15, 2022): 1–14. http://dx.doi.org/10.37745/ijeer.13vo10n2pp114.

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The objective of this research is to isolate and identify hydrocarbon-degrading bacteria for biopolymer synthesis and application in the augmentation of Nigerian heavy crude oil recovery. MEOR refers to the process of injecting either indigenous or non-indigenous microbes into hydrocarbon reserves. Injecting microorganisms with nutritional broth facilitate the formation of essential metabolites such as biosurfactants, biopolymers, and gases, resulting in decreased interfacial tension, viscosity modification, and mobility control. It is environmentally friendly, less expensive to implement, and requires minimal or no changes to the existing infrastructure. A soil sample from a hydrocarbon-contaminated site in Ogoniland was collected and sent to a laboratory for physicochemical and microbiological investigation. Bacillus sp, Pseudomonas sp, and Klebsiella sp were biochemically identified after screening three isolates for biopolymer production using Sudan black solution. To assess the ideal growth and biopolymer synthesis capability under reservoir conditions, a variety of pH, temperature, salinity, carbon, and nitrogen nutrition sources were applied to selected microorganisms. Peptone is the optimal nitrogen source for Bacillus sp, glucose is the optimal carbon source for Bacillus sp, and glycerol is the optimal carbon source for Pseudomonas sp and Klebsiella sp, as indicated by the results. In addition, the following are the ideal parameter ranges for the three microorganisms: pH 7–8, a temperature range between 25 and 350 degrees Celsius, and a salinity range between 0.5 and 5% are all desirable conditions for a body of water. After inoculation with microorganisms and the optimum nutrient source, an additional recovery range of 18.33% to 29.09% of the pore capacity was achieved. The post-recovery analysis uncovered a remarkable transformation of heavy crude to light hydrocarbon components by an average of 20.33 percent with glucose and 97.27 percent with peptone.

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Ayyed, Abbas Kadhim. "Converting Carbon Dioxide in to Methane Gas and Enhance Oil Recovery by using Biotechnology Process." Journal of Petroleum Research and Studies 12, no. 1 (March 20, 2022): 242–66. http://dx.doi.org/10.52716/jprs.v12i1.601.

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Using biotechnology in petroleum industry has many advantages .for example , Microbiologically Enhanced Oil Recovery (MEOR) increase of the productivity of the oil field and decrease the viscosity of the crude oil . It's known that atmosphere has considerable amount of CO2 gas as a result of industrial activities (crude oil production). CO2 gas plays a role in increasing atmosphere temperature and causing global warming. Bioremediation is a viable Biotechnology function for Re-producing depleted wells and global warming. It means Bioremediation uses metabolic adaptation of microorganism, a promising approach, using this technique employs of Methanogenic bacteria to convert CO2 gas in to CH4. Therefore turns carbon dioxide in to carbon which is added to crude oil (so contribute decrease the viscosity for heavy crude oil, This mechanism is a part of the promotion of oil production.it is apart an operation EOR. and Second reacts with Hydrogen by Bacteria to produce methane gas. The aim study, advantage this method increase production. and removal global warming. In this review, we discuss the role of Methanogenic bacteria in transforming CO2 gas into methane gas , that it has a role in crude oil production . Methanogenic bacteria have an important role in petroleum industry and environment during decreasing CO2 amount in the atmosphere and increasing reservoir pressure. MEOR technology uses strains that have a role in crude oil production; these bacterial strains produce biogases (Methane) that increase reservoir pressure. In this study, six strains were isolated from Rumaila oilfield, south of Iraq. These strains were identified based on microscopic and morphological observations. These strains were Methanogenic bacteria. The main part of this study includes identification of bacteria that can consume CO2 gas and making continual lab experiments to isolate and determine the best genus to do this process in oil field. Experiments were done in specific bio-labs for two years, Methanogenic bacteria strains were isolated by using specific selective growth media. The second part of this study is using these strains for bioremediation process of oil wells, which includes providing anaerobic conditions for these strains to transform CO2 gas to methane . Morphological and microscopic observations were conducted to these strains and showed the best kind of these strains according to the ability of transformation of CO2 to methane. The isolated bacteria were called BRS11 strain showed high efficiency in transformation of CO2 to methane.

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Brown, Lewis R. "Microbial enhanced oil recovery (MEOR)." Current Opinion in Microbiology 13, no. 3 (June 2010): 316–20. http://dx.doi.org/10.1016/j.mib.2010.01.011.

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Lazar, I., I. G. Petrisor, and T. F. Yen. "Microbial Enhanced Oil Recovery (MEOR)." Petroleum Science and Technology 25, no. 11 (November 27, 2007): 1353–66. http://dx.doi.org/10.1080/10916460701287714.

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Alias, Nur Hashimah, Mohd Sabri Zulkifli, Shareena Fairuz Abdul Manaf, Effah Yahya, Nurul Aimi Ghazali, and Tengku Amran Tengku Mohd. "Saccharomyces cerevisiae from Baker’s Yeast for Lower Oil Viscosity and Beneficial Metabolite to Improve Oil Recovery: An Overview." Applied Mechanics and Materials 625 (September 2014): 522–25. http://dx.doi.org/10.4028/www.scientific.net/amm.625.522.

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This article is an overview of microbial enhanced oil recovery (MEOR) and the potential ofSaccharomycesCerevisiaeto be applied in MEOR. MEOR may have same mechanisms with commercial enhanced oil recovery (EOR) but it used biological approach in improving oil recovery.SaccharomycesCerevisiaeproduced carbon dioxide and ethanol under anaerobic condition. The carbon dioxide and ethanol that produced by this microbe are two from the six main MEOR agents in improving oil recovery. This articles also discussed on previous MEOR pilot projects that were conducted in Argentina, China and Malaysia.

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She, Haicheng, Debin Kong, Yiqiang Li, Zaiqiang Hu, and Hu Guo. "Recent Advance of Microbial Enhanced Oil Recovery (MEOR) in China." Geofluids 2019 (April 9, 2019): 1–16. http://dx.doi.org/10.1155/2019/1871392.

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Compared with other enhanced oil recovery (EOR) techniques like gas flooding, chemical flooding, and thermal production, the prominent advantages of microbial enhanced oil recovery (MEOR) include environment-friendliness and lowest cost. Recent progress of MEOR in laboratory studies and microbial flooding recovery (MFR) field tests in China are reviewed. High biotechnology is being used to investigate MFR mechanisms on the molecular level. Emulsification and wettability alternation due to microbial effects are the main interests at present. Application of a high-resolution mass spectrum (HRMS) on MEOR mechanism has revealed the change of polar compound structures before and after oil degradation by the microbial on the molecular level. MEOR could be divided into indigenous microorganism and exogenous microorganism flooding. The key of exogenous microorganism flooding was to develop effective production strains, and difficulty lies in the compatibility of the microorganism, performance degradation, and high cost. Indigenous microorganism flooding has good adaptation but no follow-up process on production strain development; thus, it represents the main development direction of MEOR in China. More than 4600 wells have been conducted for MEOR field tests in China, and about 500 wells are involved in MFR. 47 MFR field tests have been carried out in China, and 12 field tests are conducted in Daqing Oilfield. MFR field test’s incremental oil recovery is as high as 4.95% OOIP, with a typical slug size less than 0.1 PV. The input-output ratio can be 1 : 6. All field tests have shown positive results in oil production increase and water cut reduction. MEOR screening criteria for reservoirs in China need to be improved. Reservoir fluid, temperature, and salinity were the most important three parameters. Microbial flooding technology is mature in reservoirs with temperature lower than 80°C, salinity less than 100,000 ppm, and permeability above 5 mD. MFR in China is very close to commercial application, while MFR as quaternary recovery like those in post-polymer flooding reservoirs needs further study.

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Heenan, Jeffrey, Abigail Porter, Dimitrios Ntarlagiannis, Lily Y. Young, Dale D. Werkema, and Lee D. Slater. "Sensitivity of the spectral induced polarization method to microbial enhanced oil recovery processes." GEOPHYSICS 78, no. 5 (September 1, 2013): E261—E269. http://dx.doi.org/10.1190/geo2013-0085.1.

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The demand for more efficient and economic oil recovery techniques has driven research into novel extraction approaches, including microbial enhanced methods. Microbial enhanced oil recovery (MEOR) is an underutilized technology that could significantly enhance tertiary oil recovery. Previous research has shown the spectral induced polarization (SIP) method to be sensitive to microbial degradation of hydrocarbons, so the method should therefore be sensitive to MEOR treatments. To test this hypothesis, heavy-oil-containing sands were monitored for a period of approximately six months while undergoing MEOR treatment. SIP monitoring showed significant sensitivity to biodegradation induced changes. Increases in phase and imaginary conductivity, with a polarization peak centered on approximately 3–8 Hz, were observed for the two MEOR active columns. Similarly, the normalized chargeability, an integrated parameter of a Debye decomposition analysis of the spectra, showed a linear increase in time. Chromatographic methods confirmed oil biodegradation in the active columns. The SIP responses are likely the result of microbial processes and the changes they promote to oil properties, such as altering wettability, or possibly the effect of organic acid production. The results of this experiment indicate that SIP may be a viable method of monitoring MEOR processes.

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Kussuryani, Yanni. "Studi Microbial Enhanced Oil Recovery Skala Laboratorium Dan Penerapannya Di Lapangan Minyak." Lembaran publikasi minyak dan gas bumi 50, no. 1 (April 24, 2016): 49–56. http://dx.doi.org/10.29017/lpmgb.50.1.730.

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Microbial Enhanced Oil Recovery (MEOR), merupakan teknologi yang dapat meningkatkan perolehan minyak dengan memanfaatkan aktivitas mikroba. Kegiatan penelitian MEOR dimulai dari isolasi dan identifi kasi mikroba; uji tabung guna menentukan mikroba dan nutrisi yang cocok untuk reservoir tertentu; uji Microbial Core Flooding/MCF dan uji coba MEOR di lapangan. Melalui uji tabung dan MCF, telah diperoleh mikroba dan nutrisi potensial yaitu kultur campuran dari sumur LDK 230 dengan starter KKL 11 dan medium M4 plus. Namun untuk uji coba lapangan berbagai faktor seperti kesesuaian antara karakteristik reservoir, kinerja mikroba dalam menghasilkan bioproduk, dan kondisi operasi masih perlu dipertimbangkan untuk keberhasilan teknologi MEOR, sehingga dapat diimplementasikan untuk peningkatkan perolehan minyak.

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Mohammadi, Mohammad Hamid, Yernazarova Aliya Kulakhmetovna, Ulzhan Shaimerdenova, Reza Joia, and Eidi Mohammad Ghafoori. "Revolutionizing Oil Extraction: Lechinysin's Potential in Microbial Enhanced Oil Recovery as a Biosurfactant." European Journal of Theoretical and Applied Sciences 2, no. 2 (March 1, 2024): 506–13. http://dx.doi.org/10.59324/ejtas.2024.2(2).43.

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As conventional oil recovery techniques have numerous deficiencies in oil recovery rate (up to 40% OOIP), process safety, financial aspects, sustainability and environmental impacts other efficient techniques like MEOR had been invented that utilize microbes or their metabolites like biosurfactants to enhance oil recovery process from depleted reservoirs and increase the recovery rate up to 50% of remained oil in the reservoirs. Biosurfactants are the interesting chemicals that encompass a large group of compounds with unique properties to play crucial role in improving oil recovery. Among biosurfactants, lichenysin produced by B. lichenoformis or B. mojavensis Jf-2 and it has several different variants based on the producing strains. It is an alternative candidate with amazing features like stability in extremely high temperature up to 140 °C, saving its optimal activity in a wide range of pH values from 6 up to 10 pH, high salinity up to 10% NaCl concentration, and a significant CMC from 10 to 20 mg/L that is the lowest CMC among studied biosurfactants suitable for MEOR. All these characteristics indicate its signifance as a biosurfactant that has the capability to revolutionize the MEOR technique in the future.

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Mohammadi, Mohammad Hamid, Yernazarova Aliya Kulakhmetovna, and Reza Joia. "An Overview of Oil Recovery Techniques: From Primary to Enhanced Oil Recovery Methods." Journal for Research in Applied Sciences and Biotechnology 3, no. 1 (March 27, 2024): 291–301. http://dx.doi.org/10.55544/jrasb.3.1.48.

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As we all know, numerous methods have been invented for better managing of the reservoirs to recover the trapped oil from them as much as possible. These techniques included primary techniques that were implemented primarily at the beginning of this industry. As these techniques were not effective enough, secondary techniques, like; water flooding and gas injection methods were created and the amount of recovered oil were increased, as well. On the contrary, the demand for more oil was raised up and it was felt that much more effective techniques are necessary. It resulted to creation of Enhanced Oil Recovery Techniques and these techniques are included; thermal methods (steam injection, steam assisted gravity drainage and in-situ combustion), Chemical methods (alkali flooding, surfactant flooding, polymer flooding, foam flooding, and combination of alkali-surfactant-polymer flooding), and microbial EOR. The most promising technique is microbial EOR because of being cost-effective and ecofriendly. GEMEOR (Genetically Engineered MEOR) and EEOR (Enzyme Enhanced Oil Recovery) are two new trends of MEOR that own potential hopes in petroleum industry.

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Jeong, Moon Sik, Young Woo Lee, Hye Seung Lee, and Kun Sang Lee. "Simulation-Based Optimization of Microbial Enhanced Oil Recovery with a Model Integrating Temperature, Pressure, and Salinity Effects." Energies 14, no. 4 (February 20, 2021): 1131. http://dx.doi.org/10.3390/en14041131.

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The microbial enhanced oil recovery (MEOR) method is an eco-friendly and economical alternative technology. The technology involves a variety of uncertainties, and its success depends on controlling microbial growth and metabolism. Though a few numerical studies have been carried out to reduce the uncertainties, no attempt has been made to consider temperature, pressure, and salinity in an integrated manner. In this study, a new modeling method incorporating these environmental impacts was proposed, and MEOR analysis was performed. As a result, accurate modeling was possible to prevent overestimating the performance of MEOR. In addition, oil recovery was maximized through sensitivity analysis and optimization based on an integrative model. Finally, applying MEOR to an actual reservoir model showed a 7% increase in oil recovery compared to waterflooding. This result proved the practical applicability of the method.

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Quraishi, Marzuqa, Shashi Kant Bhatia, Soumya Pandit, Piyush Kumar Gupta, Vivek Rangarajan, Dibyajit Lahiri, Sunita Varjani, Sanjeet Mehariya, and Yung-Hun Yang. "Exploiting Microbes in the Petroleum Field: Analyzing the Credibility of Microbial Enhanced Oil Recovery (MEOR)." Energies 14, no. 15 (August 2, 2021): 4684. http://dx.doi.org/10.3390/en14154684.

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Crude oil is a major energy source that is exploited globally to achieve economic growth. To meet the growing demands for oil, in an environment of stringent environmental regulations and economic and technical pressure, industries have been required to develop novel oil salvaging techniques. The remaining ~70% of the world’s conventional oil (one-third of the available total petroleum) is trapped in depleted and marginal reservoirs, and could thus be potentially recovered and used. The only means of extracting this oil is via microbial enhanced oil recovery (MEOR). This tertiary oil recovery method employs indigenous microorganisms and their metabolic products to enhance oil mobilization. Although a significant amount of research has been undertaken on MEOR, the absence of convincing evidence has contributed to the petroleum industry’s low interest, as evidenced by the issuance of 400+ patents on MEOR that have not been accepted by this sector. The majority of the world’s MEOR field trials are briefly described in this review. However, the presented research fails to provide valid verification that the microbial system has the potential to address the identified constraints. Rather than promising certainty, MEOR will persist as an unverified concept unless further research and investigations are carried out.

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Khire, J. M., and M. I. Khan. "Microbially enhanced oil recovery (MEOR). Part 1. Importance and mechanism of MEOR." Enzyme and Microbial Technology 16, no. 2 (February 1994): 170–72. http://dx.doi.org/10.1016/0141-0229(94)90081-7.

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Xiao, Hui, Zulhelmi Amir, and Mohd Usman Mohd Junaidi. "Development of Microbial Consortium and Its Influencing Factors for Enhanced Oil Recovery after Polymer Flooding: A Review." Processes 11, no. 10 (September 27, 2023): 2853. http://dx.doi.org/10.3390/pr11102853.

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After polymer flooding, substantial oil and residual polymers remain in reservoirs, leading to plugging and reduced recovery. MEOR (Microbial Enhanced Oil Recovery) aims to release trapped oil by utilizing microorganisms and their byproducts. The microorganisms can use residual HPAM (hydrolyzed polyacrylamide) as an energy source for polymer degradation, addressing reservoir plugging issues and improving oil recovery. However, microorganisms are sensitive to environmental conditions. This paper presents a detailed update of MEOR, including microbial products, mechanisms, and merits and demerits. The effect of the displacement fluid and conditions on microorganisms is thoroughly demonstrated to elucidate their influencing mechanism. Among these factors, HPAM and crosslinkers, which have significant biological toxicity, affect microorganisms and the efficiency of MEOR. Limited research exists on the effect of chemicals on microorganisms’ properties, metabolism, and oil displacement mechanisms. The development of microbial consortium, their metabolic interaction, and oil displacement microprocesses are also discussed. In addition, prior studies lack insights into microorganisms’ interaction and mechanisms using chemicals. Finally, field trials exist to examine the microbial consortium’s efficiency and introduce new technologies. This review mainly explores the influencing factors on microorganisms, and confirms the credibility of MEOR after polymer flooding, providing a scientific basis for improving the theory of MEOR.

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Sharma, Sagar. "The Futuristic Approach for Enhanced Oil Recovery, MEOR." International Journal for Research in Applied Science and Engineering Technology 7, no. 6 (June 30, 2019): 2217–23. http://dx.doi.org/10.22214/ijraset.2019.6372.

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Udosoh, Nsisong Emmanuel, and Thaddeus Chidiebere Nwaoha. "Demonstration of MEOR as an alternative enhanced oil recovery technique in Nigeria offshore oilfield." Journal of Mechanical and Energy Engineering 4, no. 3 (December 10, 2020): 277–84. http://dx.doi.org/10.30464/jmee.2020.4.3.277.

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Eni oilfield has been experiencing production decline with increase in water output. The implementation of enhanced oil recovery (EOR) can help to extract some percentages of the original oil in place (OOIP). EOR methods are capital intensive and few are environmental hazardous. In a bid to address these issues, this paper discusses on an alternate economically viable enhanced oil recovery technique which has the potential to curb the challenges of other conventional EOR methods.This work suggested a 3 stage approach of applying microbial enhanced oil recovery (MEOR) method for oil recovery in Eni field. The reservoir characteristics in Eni field were studied, the average porosity value of the reservoirs is between 0.238 and 0.241.The reservoir characteristics and parameters were found suitable for the application of MEOR technique for effective oil drainage from the delineated reservoir compartments. Microbial flooding was found to be matured in reservoirs with temperature less than 200 F, brine salinity not more than 100,000 ppm, water depth not more than 3500m and permeability should be above 30 (Md). It was recommended that MEOR approach should be applied in Eni Oilfield for increment of oil production and reduction in water cut due to its efficiency and economic viability.

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Zahner, R. L. L., S. J. J. Tapper, B. W. G. W. G. Marcotte, and B. R. R. Govreau. "Lessons Learned From Applications of a New Organic-Oil-Recovery Method That Activates Resident Microbes." SPE Reservoir Evaluation & Engineering 15, no. 06 (December 6, 2012): 688–94. http://dx.doi.org/10.2118/145054-pa.

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Summary Using a breakthrough process, which does not require microbes to be injected, more than 100 microbial enhanced-oil-recovery (MEOR) treatments were conducted from 2007 to the end of 2010 in oil-producing and water-injection wells in the United States and Canada. On average, these treatments increased oil production by 122%, with an 89% success rate. This paper reviews the MEOR process, reviews the results of the first 100+ treatments, and shares what has been learned from this work. Observations and conclusions include the following: Screening reservoirs is critical to success. Identifying reservoirs where appropriate microbes are present and oil is movable is the key. MEOR can be applied to a wide range of oil gravities. MEOR has been applied successfully to reservoirs with oil gravity as high as 41° API and as low as 16° API. When microbial growth is appropriately controlled, reservoir plugging or formation damage is no longer a risk. Microbes reside in extreme conditions and can be manipulated to perform valuable in-situ "work." MEOR has been applied successfully at reservoir temperatures as high as 200°F and salinities as high as 140,000 ppm total dissolved solids (TDS). MEOR can be applied successfully in dual-porosity reservoirs. A side benefit of applying MEOR is that it can reduce reservoir souring. An oil response is not always observed when treating producing wells. MEOR can be applied to many more reservoirs than thought originallys with little downside risk. This review of more than 100 MEOR well treatments expands the types of reservoirs in which MEOR can be applied successfully. Low-risk and economically attractive treatments can be accomplished when appropriate scientific analysis and laboratory screening are performed before treatments.

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Dong, Hao, Anying Zheng, Yanlong He, Xiaotong Wang, Yang Li, Gaoming Yu, Yongan Gu, et al. "Optimization and characterization of biosurfactant produced by indigenous Brevibacillus borstelensis isolated from a low permeability reservoir for application in MEOR." RSC Advances 12, no. 4 (2022): 2036–47. http://dx.doi.org/10.1039/d1ra07663a.

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Siami, Desy Hikmatul, and Novi Hery Yono. "Microbial Enhanced Oil Recovery (MEOR): Alternatif Peningkatan Produksi Migas di Indonesia." Jurnal Nasional Pengelolaan Energi MigasZoom 2, no. 2 (December 5, 2020): 01–08. http://dx.doi.org/10.37525/mz/2020-2/253.

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The need for petroleum is increasing along with the development of the industry, while the production results from the process of recovering oil from the reservoir by using primary recovery and secondary recovery are still very low so that it takes an advanced stage, namely tertiary recovery or, known as EOR. EOR is a method that produces oil production above 50%. EOR is an effort to increase oil production, so it is included in the IOR (Improved Oil Recovery) section. EOR consists of various applications, ranging from water injection, chemical injection, gases injection to microbiology injection. The stages in the injection of water and gas still leave oil trapped in the rocks in the reservoir. MEOR is one method that can be used to bring oil trapped in reservoir rocks to the surface. The effectiveness of the MEOR method is measured based on several parameters that is formation temperature, oil viscosity, permeability, saltwater salinity, water cut, API gravity crude oil, pH, pressure, residual oil saturation, porosity depth and bacterial content in the reservoir.

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Prasad, Niraj, Sumita Dasgupta, Mousumi Chakraborty, and Smita Gupta. "Microbial Enhanced Oil Recovery (MEOR) Surfactant from Pseudomonas aeruginosa isolated from Automobile Garage soil." International Journal for Research in Applied Science and Engineering Technology 10, no. 7 (July 31, 2022): 5086–96. http://dx.doi.org/10.22214/ijraset.2022.45784.

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Abstract: Microbial enhanced oil recovery (MEOR), is gaining attention today for being environmentally friendly, economically attractive, demonstrating improvement in recovery of oil entrapped in porous media. It is considered to be more efficient than other EOR methods when applied to carbonate oil reservoirs. In the current study oil degrading bacteria was isolated from soil of automobile garage. The biosurfactant producing organism was screened and characterized. 16SrRNA sequence of the most potent bacterial strain suggests it to be belonging to the genus Pseudomonas and species aeruginosa. The biosurfactant produced by the bacteria was detected as rhamnolipid with emulsification index of 69%, foaming of 57.69% and interfacial surface tension of 0.6 mN/m. The MEOR column assay revealed that the additional oil recovery for sand1 and sand2 was 62.142% and 52.173 respectively.

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Shibulal, Biji, Saif N. Al-Bahry, Yahya M. Al-Wahaibi, Abdulkader E. Elshafie, Ali S. Al-Bemani, and Sanket J. Joshi. "Microbial Enhanced Heavy Oil Recovery by the Aid of Inhabitant Spore-Forming Bacteria: An Insight Review." Scientific World Journal 2014 (2014): 1–12. http://dx.doi.org/10.1155/2014/309159.

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Crude oil is the major source of energy worldwide being exploited as a source of economy, including Oman. As the price of crude oil increases and crude oil reserves collapse, exploitation of oil resources in mature reservoirs is essential for meeting future energy demands. As conventional recovery methods currently used have become less efficient for the needs, there is a continuous demand of developing a new technology which helps in the upgradation of heavy crude oil. Microbial enhanced oil recovery (MEOR) is an important tertiary oil recovery method which is cost-effective and eco-friendly technology to drive the residual oil trapped in the reservoirs. The potential of microorganisms to degrade heavy crude oil to reduce viscosity is considered to be very effective in MEOR. Earlier studies of MEOR (1950s) were based on three broad areas: injection, dispersion, and propagation of microorganisms in petroleum reservoirs; selective degradation of oil components to improve flow characteristics; and production of metabolites by microorganisms and their effects. Since thermophilic spore-forming bacteria can thrive in very extreme conditions in oil reservoirs, they are the most suitable organisms for the purpose. This paper contains the review of work done with thermophilic spore-forming bacteria by different researchers.

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Fujiwara, Kazuhiro, Yuichi Sugai, and Heiji Enomoto. "Recent advances and prospects in Microbial Enhanced Oil Recovery (MEOR)-Outline of MEOR and microorganisms for MEOR-." Journal of the Japanese Association for Petroleum Technology 73, no. 3 (2008): 244–53. http://dx.doi.org/10.3720/japt.73.244.

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Khire, J. M., and M. I. Khan. "Microbially enhanced oil recovery (MEOR). Part 2. Microbes and the subsurface environment for MEOR." Enzyme and Microbial Technology 16, no. 3 (March 1994): 258–59. http://dx.doi.org/10.1016/0141-0229(94)90052-3.

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Cheng, Mingming, Long Yu, Jianbo Gao, Guanglun Lei, and Zaiwang Zhang. "Isolating, identifying and evaluating of oil degradation strains for the air-assisted microbial enhanced oil recovery process." PLOS ONE 16, no. 1 (January 25, 2021): e0243976. http://dx.doi.org/10.1371/journal.pone.0243976.

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Due to the inefficient reproduction of microorganisms in oxygen-deprived environments of the reservoir, the applications of microbial enhanced oil recovery (MEOR) are restricted. To overcome this problem, a new type of air-assisted MEOR process was investigated. Three compounding oil degradation strains were screened using biochemical experiments. Their performances in bacterial suspensions with different amounts of dissolved oxygen were evaluated. Water flooding, microbial flooding and air-assisted microbial flooding core flow experiments were carried out. Carbon distribution curve of biodegraded oil with different oxygen concentration was determined by chromatographic analysis. The long-chain alkanes are degraded by microorganisms. A simulation model was established to take into account the change in oxygen concentration in the reservoir. The results showed that the optimal dissolved oxygen concentration for microbial growth was 4.5~5.5mg/L. The main oxygen consumption in the reservoir happened in the stationary and declining phases of the microbial growth systems. In order to reduce the oxygen concentration to a safe level, the minimum radius of oxygen consumption was found to be about 145m. These results demonstrate that the air-assisted MEOR process can overcome the shortcomings of traditional microbial flooding techniques. The findings of this study can help for better understanding of microbial enhanced oil recovery and improving the efficiency of microbial oil displacement.

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Putri, Naya Prakasita, Cut Nanda Sari, and Misri Gozan. "Biosurfactant Screening of Halomonas meridiana BK-AB4 for Microbial Enhanced Oil Recovery." Materials Science Forum 988 (April 2020): 95–100. http://dx.doi.org/10.4028/www.scientific.net/msf.988.95.

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Biosurfactant is produced by a certain microorganism to reduce surface tension. Microbial enhanced oil recovery (MEOR) is one of the many applications of biosurfactant. However, the biosurfactant for MEOR needs to be able to withstand the extreme environment of oil reservoirs with high temperature and high salinity. Halomonas meridiana BK-AB4 is a halophilic bacterium obtained from the Bledug Kuwu crater in Central Java, Indonesia. The similarity of both environment condition indicates the potential to produce suitable biosurfactant. This study evaluates the potential of Halomonas meridiana BK-AB4 in producing biosurfactant compared to several bacteria isolated from the crater. The blood agar test of Halomonas meridiana BK-AB4 exhibited greenish discoloration around the colony, indicating the ability of type α-hemolysis. The interfacial tension was measured using the Du Nouy ring method to represent the biosurfactant activity, with the results of 0.014 dyne/cm. These results showed that Halomonas meridiana BK-AB4 has a very good potential for producing biosurfactant for MEOR. The optimum time of the culture starter for production is 6 hours, as determined from the bacterial growth curve.

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Krüger, M., J. Sitte, E. Biegel, H. Alkan, and A. Herold. "Characterisation of Indigenous Oil Field Microorganisms for Microbially Enhanced Oil Recovery (MEOR)." Chemie Ingenieur Technik 86, no. 9 (August 28, 2014): 1485. http://dx.doi.org/10.1002/cite.201450703.

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Kadarwati, S., M. Udiharto, Noegroho Hadi Hs, and Indria Doria. "SELECTED INDONESIAN MICROBES POTENTIALS FOR MEOR." Scientific Contributions Oil and Gas 25, no. 3 (March 31, 2022): 21–30. http://dx.doi.org/10.29017/scog.25.3.1071.

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Oil recovery can be increased through the activities of microbes in a process known as Microbial Enhanced Oil Recovery (MEOR). MEOR technology has been implemented in a number of oil producing companies and has proven to have a good prospect, environmentally friendly and low cost. The microbes which proliferate in Indonesian oil fields should be subjected to laboratory identification. Samples of formation water, oil, and soil were taken from various oil fields. These oil fields were selected on account of their reservoir temperatures which promise optimum growth of microbes. In order that MEOR can be applied in these oil fields, the existing microbes in their oil wells were isolated and identified. Based on the results of isolation and identification activities several indigenous bacteria species were obtained from the oil well environment. The potential of each bacteria species for use in MEOR process depends on their ability to live and grow in the reservoir environment as well as the bioproducts produced, such as biosurfactant, bioacid, and biosolvent. The bioproducts produced depend on the inherent capability of the isolate as well as the support of the medium and environmental condition. From the tests of their capability to grow in hydrocarbons, and live in semianaerobic condition, 12 isolates, were selected and some isolates were found to produce such bioproducts. The selected microbes and nutrient have been experimented by using microbial core flooding apparatus. The result has a good prospect for implementation in the oil field.

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S.J., Geetha, Ibrahim M. Banat, and Sanket J. Joshi. "Biosurfactants: Production and potential applications in microbial enhanced oil recovery (MEOR)." Biocatalysis and Agricultural Biotechnology 14 (April 2018): 23–32. http://dx.doi.org/10.1016/j.bcab.2018.01.010.

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Sun, Gangzheng, Jing Hu, Zenglin Wang, Ximing Li, and Weidong Wang. "Dynamic investigation of microbial activity in microbial enhanced oil recovery (MEOR)." Petroleum Science and Technology 36, no. 16 (May 10, 2018): 1265–71. http://dx.doi.org/10.1080/10916466.2018.1468776.

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Santos, Erick de Aquino, Manoel Jerônimo Moreira Cruz, Eddy José Francisco de Oliveira, Olivia Maria Cordeiro de Oliveira, Antônio Fernando de Souza Queiroz, Sarah Adriana Rocha Soares, and Danusia Ferreira Lima. "Microbial enhanced oil recovery (MEOR) by Pseudomonas sp. under laboratory conditions." Revista Eletrônica em Gestão, Educação e Tecnologia Ambiental 26 (January 17, 2023): e11. http://dx.doi.org/10.5902/2236117071814.

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The purpose of this work was to propose sustainable solutions for advanced oil recovery by evaluating the ability of the bacterium Pseudomonas sp. in the biotransformation of alkanes, in addition to determining strain growth patterns under extreme conditions. For this, the work was initially carried out under laboratory conditions, in which the crude oil was fractionated to obtain the saturated fraction used in the experiment. The bacterial tolerance to salinity and temperature was also tested to determine the experimental conditions and set up the experiment in regard to these parameters. Additionally, an experiment was performed to produce a biosurfactant through biostimulation. The biotransformation experiment consisted of a triplicate with treatment and a control. For treatments, Erlenmeyers flasks received 100 mL of broth containing the biosurfactant, 10 g (10%) of NaCl, 3% of the strain and 1% of the saturated fraction. Erlenmeyer flasks were incubated at 40 °C and 180 rpm for 18 days with periodic analysis. The results initially showed the bacteria exhibited better tolerance at a temperature of 40 °C, and there was no significant change for the different salinities, which was a nonlimiting parameter. For the final experiment, the bacterial growth analysed by Optical Density (OD). exhibited a low variation, in which the lowest point was in T18 with an absorbance of 0.115 and the highest point was in T6 with an absorbance of 0.149. In the qPCR analysis of the bacterial population, the pattern found was similar to the optical density results, with low variation; the lowest number of copies of the 16S rRNA gene (6.66x 103) was found in T0 and the highest number was found in T12 (7.86x 103). For biotransformation analysis, time 6 was observed to have the highest rate, with 54% oil recovery (C30), followed by 52% (C31) and 51% (C29).

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Huang, Yong Hong, Hai Yan Ding, Zhao Wei Hou, Guo Ling Ren, Xiao Lin Wu, and Kao Ping Song. "Analysis on Microbial Community Structure and Phylogenetics for the Reservoir System after the Microbial Enhanced Oil Recovery." Advanced Materials Research 361-363 (October 2011): 393–99. http://dx.doi.org/10.4028/www.scientific.net/amr.361-363.393.

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In order to probe a new enhanced oil recovery technology during the post polymer flooding process, we constructed the 16SrDNA gene library and analysed PCR-DGGE during the post-microbial enhanced oil recovery of polymer flooding process. Through the research for the reservoir system, we studied the microbial community structure and genetic diversity after polymer flooding process, made the phylogenetic analysis. It is supposed to provide a dependable basis for MEOR through directional control microbial community of reservoir, through development and application of effective microbial flooding technology.

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Zhou, Jie-fang, Guo-qiang Li, Jun-jie Xie, Xiao-yu Cui, Xiao-hui Dai, Hui-mei Tian, Pei-ke Gao, Meng-meng Wu, and Ting Ma. "A novel bioemulsifier from Geobacillus stearothermophilus A-2 and its potential application in microbial enhanced oil recovery." RSC Advances 6, no. 98 (2016): 96347–54. http://dx.doi.org/10.1039/c6ra15626f.

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YERNAZAROVA, A. K., G. K. KAIYRMANOVA, U. T. SHAIMERDENOVA, R. B. MAGMIYAYEV, and A. R. ISLAMOVA. "ИДЕНТИФИКАЦИЯ АБОРИГЕННЫХ КУЛЬТУР МИКРООРГАНИЗМОВ РОДА BACILLUS И ИХ ПОТЕНЦИАЛЬНОЕ ПРИМЕНЕНИЕ ДЛЯ УВЕЛИЧЕНИЯ НЕФТЕОТДАЧИ ПЛАСТОВ." МИКРОБИОЛОГИЯ ЖӘНЕ ВИРУСОЛОГИЯ, no. 1(44) (March 20, 2024): 158–75. http://dx.doi.org/10.53729/mv-as.2024.01.10.

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The present study was conducted to isolate active Bacillus cultures from developed oil reservoirs as potential objects for the development of microbial enhanced oil recovery (MEOR) methods. Oil formation water from two oil fields "Zhetybai" and "Kulsary" located in Western Kazakhstan were used as study materials. 8 cultures of Bacillus genus were isolated and identified on the basis of phylogenetic analysis of 16S rRNA gene. The formation of acetic acid and biosurfactants were studied as target properties valuable for MEOR. It was revealed that the investigated microorganisms are able to synthesize acetic acid as well as fatty acids. Emulsifying activity was determined as an indicator of the presence of biosurfactants. The maximum emulsification index was observed in strains of Bacillus sp. ZhM-3 and Bacillus sp. KM-2 for crude oil - 80% and 65%, for hexane - 69% and 50%, respectively. As a result of this work it was shown that the cultures of Bacillus sp. ZhM-3 and Bacillus sp. KM-2 are active producers of organic acids (acetic and butyric acids) and biosurfactants capable of emulsifying crude oil, which makes them potentially effective for application in biotechnological processes aimed at enhancing oil recovery from depleted fields. Настоящее исследование проведено с целью выделения из разработанных нефтепластов активных культур рода Bacillus, как потенциальных объектов для разработки микробиологических методов увеличения нефтеотдачи (Microbial enhanced oil recovery – MEOR). В качестве материалов исследования использована нефтепластовая вода двух нефтяных месторождений "Жетыбай" и "Кульсары", расположенных в Западном Казахстане. Выделены и идентифицированы на основе филогенетического анализа гена 16S рРНК 8 культур рода Bacillus. В качестве целевых ценных свойств для MEOR проводили изучение продукции органических кислот и биосурфактантов. Выявлено, что исследуемые микроорганизмы способны синтезировать уксусную и масляную кислоты. В качестве показателя наличия биосурфактантов определяли индекс эмульгирования сырой нефти и гексана. Максимальный индекс эмульгирования отмечен у культур Bacillus sp. ZhM-3 и Bacillus sp. KM-2 для сырой нефти – 80% и 65%, для гексана - 69% и 50%, соответственно. В результате проведенной работы показано, что культуры Bacillus sp. ZhM-3 и Bacillus sp. KM-2 являются активными продуцентами органических кислот (уксусной и масляной) и биосурфактантов, способных к эмульгированию сырой нефти, что делает их потенциально эффективными для применения в биотехнологических процессах, направленных на повышение нефтеотдачи выработанных месторождений.

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Fachria, Rizqy. "APLIKASI BIOSURFAKTAN Bacillus subtilis ATCC 19659 DENGAN MEDIA PRODUKSI LIMBAH TAHU UNTUK ENHACED OIL RECOVERY." Jurnal Teknologi Lingkungan Lahan Basah 9, no. 2 (August 29, 2021): 101. http://dx.doi.org/10.26418/jtllb.v9i2.48221.

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Biosurfactant as secondary metabolit produced by Bacillus subtilis. It has the ability to emulsify and reduce the surface tension. Biosurfactants produced by B. subtilis is a lipopeptide. Furthermore, biosurfactant can be utilized in microbial enhanced oil recovery (MEOR). In this research, biosurfactant of B. subtilis ATCC 19 659 were evaluated. The production use Nutrient Broth (NB) and soybean liquid waste. Application of biosurfactant in oil recovery showed that biosurfactant of NB recover 2 mL crude oil and biosurfactant of soybean liquid waste medium recover 3.67 mL.

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Gaol, Calvin Lumban, Leonhard Ganzer, Soujatya Mukherjee, and Hakan Alkan. "Parameters govern microbial enhanced oil recovery (MEOR) performance in real-structure micromodels." Journal of Petroleum Science and Engineering 205 (October 2021): 108814. http://dx.doi.org/10.1016/j.petrol.2021.108814.

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Yonebayashi, Hideharu, Kenji Ono, Tadashi Chida, and Heiji Enomoto. "Microbial enhanced oil recovery, Part 2: Fundamental experiments of MEOR in laboratory." Journal of the Japanese Association for Petroleum Technology 61, no. 4 (1996): 319–24. http://dx.doi.org/10.3720/japt.61.319.

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Gassara, Fatma, Navreet Suri, and Gerrit Voordouw. "Nitrate-Mediated Microbially Enhanced Oil Recovery (N-MEOR) from model upflow bioreactors." Journal of Hazardous Materials 324 (February 2017): 94–99. http://dx.doi.org/10.1016/j.jhazmat.2015.12.039.

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Tiong, Adrian Chiong Yuh, Inn Shi Tan, Henry Chee Yew Foo, Man Kee Lam, Hisham Ben Mahmud, and Keat Teong Lee. "A mini-investigation on enhanced oil recovery evolution (2007 – 2020)." MATEC Web of Conferences 377 (2023): 01015. http://dx.doi.org/10.1051/matecconf/202337701015.

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Energy plays an important role in sustaining humanity. With rising worldwide energy demand and the great dependence of energy generation on fossil fuels, it is inevitable that enhanced oil recovery must be deployed to recover more possible reserves. This report focuses on reviewing publications related to enhanced oil recovery from 2007 to 2020 through the utilization of bibliometric analysis. Of the 5498 documents retrieved from Web of Science, 569 journals, 90 countries, 2025 organizations, and 8684 authors are involved. China, the United States, Iran, Canada, and India published the most documents. The United States has the highest h-index at 61. The analysis of keywords had shown that the hot issues lie around four main domains namely carbon capture, utilization, and sequestration (CCUS), microbial enhanced oil recovery (MEOR), development of unconventional reserves, and chemical enhanced oil recovery. This study provides some useful insights for future research directions. From there, discussions were subsequently placed on chemical EOR.

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Nazina, Tamara, Diyana Sokolova, Denis Grouzdev, Ekaterina Semenova, Tamara Babich, Salimat Bidzhieva, Dmitriy Serdukov, et al. "The Potential Application of Microorganisms for Sustainable Petroleum Recovery from Heavy Oil Reservoirs." Sustainability 12, no. 1 (December 18, 2019): 15. http://dx.doi.org/10.3390/su12010015.

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A microbial enhanced oil recovery (MEOR) technique was tested at low-temperature heavy oil reservoirs (Russia). The bioaugmentation approach used is based on the introduction of hydrocarbon-oxidizing bacteria into the oilfield in combination with an injection of oxygen as a H2O2 solution in order to initiate the first stage of hydrocarbon oxidation and of (NH4)2HPO4 as a source of biogenic elements. Before the pilot trials, the microorganisms of petroleum reservoirs were investigated by high-throughput sequencing, as well as by culture-base and radioisotope techniques. Molecular studies revealed the differences in microbial composition of the carbonate and terrigenous oil reservoirs and the communities of injection and formation water. Aerobic bacteria Rhodococcus erythropolis HO-KS22 and Gordonia amicalis 6-1 isolated from oilfields oxidized oil and produced biosurfactants. Fermentative enrichment and pure cultures produced considerable amounts of low fatty acids and alcohols from sacchariferous substrates. In core-flooding tests, 43.0–53.5% of additional heavy oil was displaced by aerobic bacteria, producing biosurfactants, and 13.4–45.5% of oil was displaced by fermentative bacteria, producing low fatty acids, alcohols, and gas. A total of 1250 t additional oil was recovered as a result of the application of an MEOR technique at the Cheremukhovskoe heavy oil reservoir and Vostochno-Anzirskoe reservoir with light conventional oil.

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Nmegbu, Chukwuma Godwin J., Edet Emmanuel A., and Chindah Nmegbu. "Laboratory Determination of Productivity of Bomu Oil Field (Oml 11 as Case Study) Using MEOR Process and Calcium Oxide Nanoparticles." International Journal of Engineering and Modern Technology 8, no. 5 (August 28, 2023): 56–70. http://dx.doi.org/10.56201/ijemt.v8.no5.2022.pg56.70.

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The need for petroleum products in the world is increasing daily, this product can only be gotten from the refining of crude oil a naturally occurring mineral resource which is produced by degradation of organic materials. This crude is gotten from several wells drilled for the sole purpose of the production of this mineral resource but this wells are being shut-down and abandoned because of the decrease in the well’s productivity which causes losses to the operators and poor finances for the petroleum industry. Therefore, the need to increase the recovery of a well is been considered, these methods are called enhanced oil recovery method. These methods include Gas injection method, Steam Injection method, Chemical injection method (chemicals such as surfactants, polymers etc) Microbial method and the use of nanoparticles as additives to help increase the recovery process. The type of enhanced oil recovery used in this work is the microbial enhanced oil recovery, this method makes use of biosurfactants which can also be referred to as microbe surfactants. The biosurfactants are gotten from microorganism they act as surfactants which reduces the interfacial tension between the molecules of the substance and the interface, this biosurfactants can be used for many industrial purposes which includes bioremediation, oil recovery etc. Nanoparticles are also another aspect of the recent technology which can increase the recovery of our well providing more crude and finance for the petroleum industry. This work shows how biosurfactants and nanoparticles when used together can increase oil recovery. The ever-rising global demand for energy and the issue of large volumes of unrecovered oil after primary and secondary oil production operations are driving the development and/or advancement of enhanced oil recovery (EOR) techniques. Conventional EOR processes include thermal, immiscible and miscible gas injection, chemical, and microbial enhanced oil recovery

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Kadnikov, Vitaly V., Nikolai V. Ravin, Diyana S. Sokolova, Ekaterina M. Semenova, Salimat K. Bidzhieva, Alexey V. Beletsky, Alexey P. Ershov, et al. "Metagenomic and Culture-Based Analyses of Microbial Communities from Petroleum Reservoirs with High-Salinity Formation Water, and Their Biotechnological Potential." Biology 12, no. 10 (October 2, 2023): 1300. http://dx.doi.org/10.3390/biology12101300.

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The reserves of light conditional oil in reservoirs with low-salinity formation water are decreasing worldwide, necessitating the extraction of heavy oil from petroleum reservoirs with high-salinity formation water. As the first stage of defining the microbial-enhanced oil recovery (MEOR) strategies for depleted petroleum reservoirs, microbial community composition was studied for petroleum reservoirs with high-salinity formation water located in Tatarstan (Russia) using metagenomic and culture-based approaches. Bacteria of the phyla Desulfobacterota, Halanaerobiaeota, Sinergistota, Pseudomonadota, and Bacillota were revealed using 16S rRNA-based high-throughput sequencing in halophilic microbial communities. Sulfidogenic bacteria predominated in the studied oil fields. The 75 metagenome-assembled genomes (MAGs) of prokaryotes reconstructed from water samples were assigned to 16 bacterial phyla, including Desulfobacterota, Bacillota, Pseudomonadota, Thermotogota, Actinobacteriota, Spirochaetota, and Patescibacteria, and to archaea of the phylum Halobacteriota (genus Methanohalophilus). Results of metagenomic analyses were supported by the isolation of 20 pure cultures of the genera Desulfoplanes, Halanaerobium, Geotoga, Sphaerochaeta, Tangfeifania, and Bacillus. The isolated halophilic fermentative bacteria produced oil-displacing metabolites (lower fatty acids, alcohols, and gases) from sugar-containing and proteinaceous substrates, which testify their potential for MEOR. However, organic substrates stimulated the growth of sulfidogenic bacteria, in addition to fermenters. Methods for enhanced oil recovery should therefore be developed, combining the production of oil-displacing compounds with fermentative bacteria and the suppression of sulfidogenesis.

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Suleimanov, Baghir A., Sabina J. Rzayeva, and Svetlana S. Keldibayeva. "A new microbial enhanced oil recovery (MEOR) method for oil formations containing highly mineralized water." Petroleum Science and Technology 38, no. 23 (July 17, 2020): 999–1006. http://dx.doi.org/10.1080/10916466.2020.1793777.

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Gu, Yi, Zhenzhen Wu, Guan Wang, Bo Zhi, Jiliang Yu, Huiqiang Fan, Suzhen Guo, Ting Ma, and Guoqiang Li. "Effects of Air Injection on the Metabolic Activity of Emulsifier-Producing Bacteria from Oil Reservoirs." Geofluids 2020 (February 10, 2020): 1–9. http://dx.doi.org/10.1155/2020/8987258.

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Oil emulsification is one of the major mechanisms for microbially enhanced oil recovery (MEOR). Although air injection is generally recommended for field trials of MEOR in China, its influence on the microbial community structure in oil reservoirs remains poorly understood, especially activation of emulsifier-producing bacteria. Herein, the effects of air injection on oil emulsification, nutrient consumption, oil properties, and microbial community structures were compared for activated cultures under four different oxygen content conditions: anaerobic, facultative anaerobic, intermittent aeration, and aerobic. The results showed that crude oil in aerobic and intermittent aeration cultures was emulsified effectively when nutrients were thoroughly depleted. The particle diameter of emulsified droplets was 4.74-10.02 μm. High-throughput sequencing results showed that Bacillus and Aeribacillus were effectively activated under aerobic and intermittent aeration conditions, while Tepidimicrobium and Coprothermobacter were activated under facultative anaerobic and anaerobic conditions. Real-time quantitative PCR results showed that the initial emulsifying effect was positively correlated with the abundance of Aeribacillus pallidus.

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Fujiwara, Kazuhiro, Yuichi Sugai, and Heiji Enomoto. "Recent advances and prospects in Microbial Enhanced Oil Recovery (MEOR)-Present and future prospects for development of MEOR technology-." Journal of the Japanese Association for Petroleum Technology 73, no. 6 (2008): 531–40. http://dx.doi.org/10.3720/japt.73.531.

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Kumdhitiahutsawakul, Ladapa, Thanachai Singhapetcharat, Paweena Choochuay, Pinan Dawkrajai, Yingmanee Tragoolpua, and Sakunnee Bovonsombut. "Biosurfactant-Producing Bacteria and Microbial Analysis in Mae Soon Reservoir, Fang Oil Field, Chiang Mai, Thailand." Chiang Mai Journal of Science 50, no. 3 (May 31, 2023): 1–14. http://dx.doi.org/10.12982/cmjs.2023.024.

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Microbial Enhanced Oil Recovery (MEOR) is an effective alternative method for oil recovery in reservoirs using microorganisms or their secondary metabolites. This research aimed to evaluate the indigenous bacteria from Mae Soon reservoir by culture-dependent and culture-independent methods and to investigate the potential of biosurfactant-producing bacteria using a drop-collapsed assay. Indigenous bacteria were isolated from the oil sands of the reserved core of Mae Soon reservoir using fi ve different media (nutrient, Luria-Bertani, mineral salt, tryptic soy, and peptone yeast extract). Fifty-four facultative anaerobic bacterial isolates were obtained. Seven isolates showed their potential as biosurfactant producers in the drop-collapse assay. Based on 16S rRNA gene analysis, six of the biosurfactant-producing bacterial isolates belonged to the species Bacillus licheniformis and one belonged to the species B. subtilis. The biosurfactant producers and microbial community in the oil sands were determined using Denaturing Gradient Gel Electrophoresis (DGGE). Interestingly, DGGE bands corresponding to bacteria belonging to the genus Geobacillus sp. were detected. Overall, the results obtained from this work showed that indigenous bacteria in Mae Soon reservoir oil well were prospective for use in MEOR.

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Shi, Guoxin, Yuanyuan Jia, Lei Bai, Guoqiang Li, Xiaoli Liu, Meichen Liu, Xuecheng Dai, Xuefeng Tian, Hongbo Wang, and Ting Ma. "Nutrient Stimulation of Indigenous Microorganisms for Oil-in-Water Emulsion in a Medium Temperature Petroleum Reservoir with Ca2+-Rich Brine." Geofluids 2021 (February 17, 2021): 1–9. http://dx.doi.org/10.1155/2021/6659038.

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One of the challenges indigenous microbial enhanced oil recovery (MEOR) is facing is the high percentage of divalent ions, which obstruct the growth and metabolism of microorganisms and destabilize the oil-in-water (o/w) emulsion. Six formulas were selected for the stimulation of indigenous microbes and to compare their performances on the oil emulsification and oil spreading in the Luliang oilfield containing Ca2+-rich brine. Illumina MiSeq sequencing of 16S rRNA genes was applied to investigate the structural response of microbial communities to various formulas. The results showed that the addition of proper organic phosphorus and the optimal P/N ratio (0.01) can facilitate production of biosurfactant and create stable o/w emulsion with specific reservoir condition containing Ca2+-rich brine, through direct stimulation of certain functional microbes. This study provides a promising path for direct enrichment of biosurfactant-producing and oil-degrading Dietzia genus and a potential instructional approach of indigenous MEOR in Luliang oilfield.

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Setyaningrum, Sinta. "Bacterial Core Flooding on Limestone Artificial Core for Microbial Enhanced Oil Recovery (MEOR) Application." PETRO:Jurnal Ilmiah Teknik Perminyakan 10, no. 4 (January 27, 2022): 197–205. http://dx.doi.org/10.25105/petro.v10i4.10811.

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Microbial Enhanced Oil Recovery (MEOR) is a tertiary methods to increase oil production using bioproduct of microorganisms form acidic compounds, gases, polymers, and surfactants, as an agent that can alter the characteristics of the reservoir rock and oil. Previous laboratory research indicated that bacterial isolates indigen from petroleum reservoir, MSD4 and MSD5, with the amount of inoculum ratio 1:1 in recovery medium can reduce the viscosity value and interfacial tension of oil. The aim of this research was to determine the amount of oil recovery from low saturated oil in limestone artificial core by using bacterial core flooding method, also to determine the physical character and chemical character changes in limestone rocks and petroleum. Each bacteria, MSD4 and MSD5, were adapted to the medium SMSS that have been added by 5% oil. Inoculum of indigen bacterial cultures to be injected into the limestone core was bred in the SMSS culture medium added molasses with a varied amount, as much as 1%, 2%, and 3%. From the those variation, the amount of molasses added as much as 1% in the medium SMSS, is known that MSD4 and MSD5 cultures can grow to the highest μ respectively at the age of 16-hour and 24-hour with a value at 0.9992 hours-1 and 0, 9993 hours-1. Inoculum MSD4 and MSD5 cultures with the amount of 1:1 ratio and each bacterial cell density 107-108 cells/mL, were injected into artificial limestone cores with oil saturated less than 15%. The value of rock porosity range used for the simulation of bacterial flooding is 37% -42%, while permeability range used low permeability value (1mD-10mD) and fair permeability value (10mD-50mD). High oil recovery factor (RF%) were obtained from bacterial flooding simulation and incubated for 7 days in a temperature of 55°C on medium SMSS for cores with low permeability values and fair, has a range of 16,3% - 32% and 12% -15,3%. The result of microorganisms activity in limestone rocks from a change on physical character, have a range at -41,8% - 698% on permeability value and a range at -63,85% - 1,03% on porosity value. Indicator of bacterial attachment on limestone cores can be determined by photomicrographs analyzing Scanning Electron Microscope and Energy Dispersive X-ray Spectroscopy.

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She, Yue Hui, Fu Chang Shu, Zheng Liang Wang, and Long Jiang Yu. "Investigation of Indigenous Microbial Enhanced Oil Recovery in a Middle Salinity Petroleum Reservoir." Advanced Materials Research 365 (October 2011): 326–31. http://dx.doi.org/10.4028/www.scientific.net/amr.365.326.

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Culture-based techniques were applied to analyze the diversity of indigenous microbial communities in the Qinghai middle salinity petroleum reservoir (QH-MSPR). The results of the most probable number (MPN) method indicated there was an abundance of indigenous microbes (105-106MPN/ml). Two isolations (BIOS682) from the QH-MSPR were identified as Brevibacillus agri and Brevibacillus levickii. The study showed that BIOS682 enhanced the degradation rate of Huatugou crude oil. The viscosity and freezing point of crude oil after treatment by BIOS682 were all decreased. The results of TLC and FTIR spectrum analysis of the biosurfactant produced by BIOS682 indicated that it was identical to that of lipopeptide. The core-flooding tests showed that the incremental oil recoveries were 7.05-10.15%. Thus, BIOS682 may provide a viable application of microbial enhanced oil recovery (MEOR).

Journal articles on the topic 'Microbiological enhanced oil recovery (MEOR)'

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