Log in

Relevant bibliographies by topics / Microbead / Journal articles

Journal articles on the topic 'Microbead'

To see the other types of publications on this topic, follow the link: Microbead.

Author: Grafiati

Published: 4 June 2021

Last updated: 10 March 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Microbead.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Ichikawa, Akihiko, Ayae Honda, Miho Ejima, Tamio Tanikawa, Fumihito Arai, and Toshio Fukuda. "In-Situ Formation of a Gel Microbead for Laser Micromanipulation of Microorganisms, DNA, and Viruses." Journal of Robotics and Mechatronics 19, no. 5 (October 20, 2007): 569–76. http://dx.doi.org/10.20965/jrm.2007.p0569.

Full text

Abstract:

We proposein situformation of gel microbeads made of a thermoreversible hydrogel for indirect laser micromanipulation of microorganisms, DNA, and viruses. Using a 1064 nm laser, we irradiated an aqueous solution mixed with poly-(N-isopropylacrylamide) through a high- magnification lens, thereby forming a gel microbead through heating at the laser focus. The gel microbead, trapped by the laser, was used to indirectly manipulate micro- and nano-scale samples. Laser tweezers stably handle micro-scale object ranging from several tens of nm to several hundreds of µm. This cannot be done with nano-scale objects of a few nm, however, due to laser beam heating. We demonstrate how to manipulate microorganisms, DNA, and viruses indirectly using a gel microbead made from an aqueous poly-(N-isopropylacrylamide) solution. We reduced laser power for gel microbead formation, and used the gel microbead trapped by the laser to manipulate microorganisms, DNA, and viruses.

APA, Harvard, Vancouver, ISO, and other styles

2

Feng, Sunlian, Lon V. Kendall, Emir Hodzic, Scott Wong, Edward Lorenzana, Kimberly Freet, Karin S. Ku, Paul A. Luciw, Stephen W. Barthold, and Imran H. Khan. "Recombinant Helicobacter bilis Protein P167 for Mouse Serodiagnosis in a Multiplex Microbead Assay." Clinical Diagnostic Laboratory Immunology 11, no. 6 (November 2004): 1094–99. http://dx.doi.org/10.1128/cdli.11.6.1094-1099.2004.

Full text

Abstract:

ABSTRACT Infection of mice with Helicobacter bilis is widespread in research and commercial mouse colonies. Therefore, sensitive, specific, and high-throughput assays are needed for rapid and accurate testing of mice in large numbers. This report describes a novel multiplex assay, based on fluorescent microbeads, for serodetection of H. bilis infection. The assay requires only a few microliters of serum to perform and is amenable to a high-throughput format. Individual microbead sets were conjugated to purified, H. bilis-specific, recombinant proteins P167C and P167D and bacterial membrane extracts from H. bilis and Helicobacter hepaticus. For detecting H. bilis infection in the microbead multiplex assay, P167C and P167D provided significantly higher sensitivities (94 and 100%, respectively) and specificities (100 and 95%, respectively) than membrane extract (78% sensitivity and 65% specificity). Microbead multiplex assay results were validated by enzyme-linked immunosorbent assay. Purified recombinant proteins showed low batch-to-batch variation; this feature allows for ease of quality control, assay robustness, and affordability. Thus, recombinant antigens are highly suitable in the multiplex microbead assay format for serodetection of H. bilis infection.

APA, Harvard, Vancouver, ISO, and other styles

3

Wells, William. "Microbead expression arrays." Genome Biology 1 (2000): spotlight—20000606–01. http://dx.doi.org/10.1186/gb-spotlight-20000606-01.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Arai, Fumihito, Toshiaki Endo, Ryuji Yamauchi, and Toshio Fukuda. "3D 6DOF Manipulation of Microbead by Laser Tweezers." Journal of Robotics and Mechatronics 18, no. 2 (April 20, 2006): 153–59. http://dx.doi.org/10.20965/jrm.2006.p0153.

Full text

Abstract:

Laser tweezers are suitable for manipulation of a single microscopic biological object. It can manipulate micro bio-object by noncontact in closed space. Single cell manipulation is important for biological research works, and 3D 6DOF manipulation (Position control and Orientation control) is useful technique in many biological experiments. Here we proposed 3D synchronized laser manipulation system by which we can manipulate multiple micro-objects along each designed trajectory in 3D space. Position and orientation of microbeads can be controlled by the newly developed 3D synchronized laser micromanipulation system. We succeeded in the orientation control of the microbead by using the laser trapped microtools. We demonstrate 3D 6DOF manipulation of the microbead by the experiment.

APA, Harvard, Vancouver, ISO, and other styles

5

Fu, Xin, He Zhang, Jie Zhang, Shi-Tong Wen, and Xing-Cheng Deng. "A Highly Sensitive and Label-Free Microbead-Based ‘Turn-On’ Colorimetric Sensor for the Detection of Mercury(II) in Urine Using a Peroxidase-Like Split G-Quadruplex–Hemin DNAzyme." Australian Journal of Chemistry 71, no. 12 (2018): 945. http://dx.doi.org/10.1071/ch18302.

Full text

Abstract:

A highly sensitive and label-free microbead-based ‘turn-on’ assay was developed for the detection of Hg2+ in urine based on the Hg2+-mediated formation of intermolecular split G-quadruplex–hemin DNAzymes. In the presence of Hg2+, T–T mismatches between the two partial cDNA strands were stabilized by a T–Hg2+–T base pair, and can cause the G-rich sequences of the two oligonucleotides to associate to form a split G-quadruplex which is able to bind hemin to form the catalytically active G-quadruplex–hemin DNAzyme. This microbead-based ‘turn-on’ process allows the detection of Hg2+ in urine samples at concentrations as low as 0.5 pM. The relative standard deviation and recovery are 1.2–3.9 and 98.7–103.2%, respectively. The remarkable sensitivity for Hg2+ is mainly attributed to the enhanced mass transport ability that is inherent in homogeneous microbead-based assays. Compared with previous developments of intermolecular split G-quardruplex–hemin DNAzymes for the homogeneous detection of Hg2+ (the limit of detection was 19nM), a signal enhancement of ~1000 times is obtained when such an assay is performed on the surface of microbeads.

APA, Harvard, Vancouver, ISO, and other styles

6

Lany, M., G. Boero, and R. S. Popovic. "Superparamagnetic microbead inductive detector." Review of Scientific Instruments 76, no. 8 (August 2005): 084301. http://dx.doi.org/10.1063/1.1988131.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Morgan, James E., and James R. Tribble. "Microbead models in glaucoma." Experimental Eye Research 141 (December 2015): 9–14. http://dx.doi.org/10.1016/j.exer.2015.06.020.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Theilacker, Nora, Eric E. Roller, Kristopher D. Barbee, Matthias Franzreb, and Xiaohua Huang. "Multiplexed protein analysis using encoded antibody-conjugated microbeads." Journal of The Royal Society Interface 8, no. 61 (January 19, 2011): 1104–13. http://dx.doi.org/10.1098/rsif.2010.0594.

Full text

Abstract:

We describe a method for multiplexed analysis of proteins using fluorescently encoded microbeads. The sensitivity of our method is comparable to the sensitivity obtained by enzyme-linked immunosorbent assay while only 5 µl sample volumes are needed. Streptavidin-coated, 1 µm beads are encoded with a combination of fluorophores at different intensity levels. As a proof of concept, we demonstrate that 27 microbead populations can be readily encoded by affinity conjugation using three intensity levels for each of three different biotinylated fluorescent dyes. Four populations of encoded microbeads are further conjugated with biotinylated capture antibodies and then combined and immobilized in a microfluidic flow cell for multiplexed protein analysis. Using four uniquely encoded microbead populations, we show that a cancer biomarker and three cytokine proteins can be analysed quantitatively in the picogram per millilitre range by fluorescence microscopy in a single assay. Our method will allow for the fabrication of high density, bead-based antibody arrays for multiplexed protein analysis using integrated microfluidic devices and automated sample processing.

APA, Harvard, Vancouver, ISO, and other styles

9

Nanda, Himansu Sekhar, Shangwu Chen, Qin Zhang, Naoki Kawazoe, and Guoping Chen. "Collagen Scaffolds with Controlled Insulin Release and Controlled Pore Structure for Cartilage Tissue Engineering." BioMed Research International 2014 (2014): 1–10. http://dx.doi.org/10.1155/2014/623805.

Full text

Abstract:

Controlled and local release of growth factors and nutrients from porous scaffolds is important for maintenance of cell survival, proliferation, and promotion of tissue regeneration. The purpose of the present research was to design a controlled release porous collagen-microbead hybrid scaffold with controlled pore structure capable of releasing insulin for application to cartilage tissue regeneration. Collagen-microbead hybrid scaffold was prepared by hybridization of insulin loaded PLGA microbeads with collagen using a freeze-drying technique. The pore structure of the hybrid scaffold was controlled by using preprepared ice particulates having a diameter range of 150–250 μm. Hybrid scaffold had a controlled pore structure with pore size equivalent to ice particulates and good interconnection. The microbeads showed an even spatial distribution throughout the pore walls.In vitroinsulin release profile from the hybrid scaffold exhibited a zero order release kinetics up to a period of 4 weeks without initial burst release. Culture of bovine articular chondrocytes in the hybrid scaffold demonstrated high bioactivity of the released insulin. The hybrid scaffold facilitated cell seeding and spatial cell distribution and promoted cell proliferation.

APA, Harvard, Vancouver, ISO, and other styles

10

Ozanich, Richard M., Kate C. Antolick, Cindy J. Bruckner-Lea, Brian P. Dockendorff, Ashton N. Easterday, Heather C. Edberg, Jay W. Grate, et al. "Use of a Novel Fluidics Microbead Trap/Flow-Cell Enhances Speed and Sensitivity of Bead-Based Bioassays." JALA: Journal of the Association for Laboratory Automation 12, no. 5 (October 2007): 303–10. http://dx.doi.org/10.1016/j.jala.2007.05.002.

Full text

Abstract:

Automated devices and methods for biological sample preparation often use surface functionalized microbeads (superparamagnetic or nonmagnetic) to allow capture, purification, and preconcentration of trace amounts of proteins, cells, or nucleic acids (DNA/RNA) from complex samples. We have developed unique methods and hardware for trapping either magnetic or nonmagnetic functionalized beads that allow samples and reagents to be efficiently perfused over a microcolumn of beads. This approach yields enhanced mass transport and up to fivefold improvements in assay sensitivity or speed, dramatically improving assay capability relative to assays conducted in more traditional “batch modes” (i.e., in tubes or microplate wells). Summary results are given that highlight the analytical performance improvements obtained for automated microbead processing systems using novel microbead trap/flow-cells for various applications including (1) simultaneous capture of multiple cytokines using an antibody-coupled polystyrene bead assay with subsequent flow cytometry detection; (2) capture of nucleic acids using oligonucleotide-coupled polystyrene beads with flow cytometry detection; and (3) capture of Escherichia coli 0157:H7 from 50-mL sample volumes using antibody-coupled superparamagnetic microbeads with subsequent culturing to assess capture efficiency.

APA, Harvard, Vancouver, ISO, and other styles

11

Gao, Yu Xin, Jia Yu Xiang, Jun Wang, and Bao Ying Yu. "Study on Mechanical Properties of Microbead Modified Super Sulfate Cement Concrete." Key Engineering Materials 629-630 (October 2014): 455–61. http://dx.doi.org/10.4028/www.scientific.net/kem.629-630.455.

Full text

Abstract:

The microbead modified super sulfate cement (SSC) was employed to prepare C60~C80 concrete, this work emphasis on mechanical properties and micro-mechanism, besides, the service behaviors of fresh concrete was tested. The SSC concrete mechanical properties influenced by particles size of microbead, slag and gypsum were discussed. Mechanisms of mechanical properties changes were elaborated from micro, meso and macro scales, mechanical model was established and relevant rules of mechanical properties development were obtained. Experiment results show that compressive strength increased with the largerer specific surface area of microbead, slag powder and gypsum. There was a promoting effect for SSC concrete hydration reaction by appropriate dosage (10%~20%) of microbead, which had prominent interstitial effect and system with excellent gradation, and fresh concrete workability was significantly improved. The compressive strength—curing ages model of high strength SSC concrete was established by half value intensity index, which coincide well with measured values. Key words: super sulphated cement concrete, microbead, mechanical properties, mechanical model, specific surface area

APA, Harvard, Vancouver, ISO, and other styles

12

Pudasaini, Sanam, A. T. K. Perera, Syed S. U. Ahmed, Yong Bing Chong, Sum Huan Ng, and Chun Yang. "An Electroporation Device with Microbead-Enhanced Electric Field for Bacterial Inactivation." Inventions 5, no. 1 (December 27, 2019): 2. http://dx.doi.org/10.3390/inventions5010002.

Full text

Abstract:

This paper presents an electroporation device with high bacterial inactivation performance (~4.75 log removal). Inside the device, insulating silica microbeads are densely packed between two mesh electrodes that enable enhancement of the local electric field strength, allowing improved electroporation of bacterial cells. The inactivation performance of the device is evaluated using two model bacteria, including one Gram-positive bacterium (Enterococcus faecalis) and one Gram-negative bacterium (Escherichia coli) under various applied voltages. More than 4.5 log removal of bacteria is obtained for the applied electric field strength of 2 kV/cm at a flowrate of 4 mL/min. The effect of microbeads on the inactivation performance is assessed by comparing the performance of the microbead device with that of the device having no microbeads under same operating conditions. The comparison results show that only 0.57 log removal is achieved for the device having no microbeads—eightfold lower than for the device with microbeads.

APA, Harvard, Vancouver, ISO, and other styles

13

Ravindran, Resmi, Imran Khan, Robert Gosselin, Ted Wun, V. V. Krishnan, Paul Luciw, and Kim Janatpour. "A Novel PF4-Heparin Microbead Assay and Plasma Protein Biomarker Profiling for Improved Diagnosis of Heparin Induced Thrombocytopenia." Blood 110, no. 11 (November 16, 2007): 3216. http://dx.doi.org/10.1182/blood.v110.11.3216.3216.

Full text

Abstract:

Abstract Heparin-induced thrombocytopenia (HIT) is a potentially life-threatening, immune-mediated complication of heparin administration resulting in a hypercoagulable state. The diagnosis is made using clinical scoring systems with confirmatory laboratory testing. However, current diagnostic criteria may lack predictive value for HIT-associated thrombosis and need for alternative anticoagulation. Therefore, there is a need to develop diagnostic methods that will have a better predictive value for HIT diagnosis. The pathogenesis of HIT involves platelet-activating antibodies that recognize platelet factor 4 (PF4) -heparin complex, and may also involve dysregulation of inflammatory mediators and growth factors from platelets and endothelial cells. Luminex multiplex microbead assay allows for the simultaneous detection of multiple serum analytes. The aim of this study is to develop a multiplex method to detect anti-PF4-heparin antibodies and to generate profiles of cytokines, chemokines, growth factors and adhesion molecules with better predictive value for the diagnosis of HIT than current diagnostic methods. In combination with measurement of antibodies to (PF4)-heparin complex, such a panel is expected to enhance the accuracy of HIT diagnosis. To develop the anti-PF4-heparin IgG microbead assay, carboxylated Luminex microbeads were coated at various concentrations with the optimal molar ratio of the PF4/polyvinyl sulfonate (PF4/PVS) complex, the antigenic determinant from the GTI ELISA kit, (provided by GTI, Waukesha WI). The anti-PF4-heparin microbead assay was standardized using plasma samples from patients and healthy controls. Furthermore, we compared the anti-PF4-heparin microbead assay to traditional commercial HIT antibody ELISA kits from GTI, Diagnostica Stago and Aniara. To identify potential plasma biomarkers for HIT, measurements were made on plasma levels of 63 cytokines, chemokines, growth factors and adhesion molecules in patients with and without HIT (as defined by the Chong’s score) and healthy controls using commercial multiplex detection panels (Bio-Rad, Upstate, Linco). Multiplex data were analyzed using multivariate statistical methods and compared with their respective clinical scores. Preliminary anti-PF4-heparin microbead assay results show an overall concordance rate of 80% with GTI (Polyclonal) ELISA; 91% with Diagnostica Stago (Polyclonal); 78% with Aniara Polyclonal and 76% with Aniara IgG ELISA. The anti-PF4-heparin microbead assay results also show good correlation with clinical determination of HIT disease status as determined by Chong’s scoring (72% sensitivity, and 58% specificity). Preliminary biomarker profiling suggest that Il-2Ra, b-NGF, GRO-a, TNF-b, PDGF AB/BB, PDGF AA, and s-ICAM1 are potentially useful markers in further analysis for distinguishing HIT status. We have identified these candidate biomarkers to evaluate in a larger number of patient samples with suspected HIT. Our data show that our novel microbead assay for anti-PF4-heparin antibodies correlate well with the existing conventional ELISA results, and also correlate with HIT diagnosis as determined by the Chong’s score. Furthermore, we have identified a panel of biomarkers that could be useful for improved diagnosis and prognosis of HIT, and will establish a basis for further understanding the pathophysiology of this coagulation disorder.

APA, Harvard, Vancouver, ISO, and other styles

14

Miles, Deon T., Andrew Knedlik, and David O. Wipf. "Construction of Gold Microbead Ultramicroelectrodes." Analytical Chemistry 69, no. 6 (March 1997): 1240–43. http://dx.doi.org/10.1021/ac960970y.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Levinbuk, M. I. "Cracking on a microbead catalyst." Chemistry and Technology of Fuels and Oils 35, no. 6 (November 1999): 355–57. http://dx.doi.org/10.1007/bf02694097.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Kuramitz, Hideki. "Magnetic microbead-based electrochemical immunoassays." Analytical and Bioanalytical Chemistry 394, no. 1 (February 20, 2009): 61–69. http://dx.doi.org/10.1007/s00216-009-2650-y.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Timmons, Michael B., John L. Holder, and James M. Ebeling. "Application of microbead biological filters." Aquacultural Engineering 34, no. 3 (May 2006): 332–43. http://dx.doi.org/10.1016/j.aquaeng.2005.07.003.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Hiki, Kyoshiro, and Fumiyuki Nakajima. "Effect of salinity on the toxicity of road dust in an estuarine amphipod Grandidierella japonica." Water Science and Technology 72, no. 6 (June 13, 2015): 1022–28. http://dx.doi.org/10.2166/wst.2015.304.

Full text

Abstract:

Urban runoff can reach coastal aquatic environments; however, little is known about the effect of salinity on road runoff toxicity. The objective of this study is to investigate the toxicity of highway road dust over a salinity gradient from 5 to 35‰, in an estuarine benthic amphipod, Grandidierella japonica. Road dust toxicity was evaluated by assessing mortality after 10 days of exposure and short-term microbead ingestion activity of the amphipod. For all road dust samples considered, amphipod mortality increased with increasing salinity, whereas no significant difference in mortality was observed among test salinities in the reference river sediment. Ingestion activity during exposure to road dust decreased with increasing salinity. In fact, none of the individuals ingested any microbeads at salinity of 35‰. If assumed microbead ingestion is a proxy for feeding activity, high mortality at 35‰ could be attributed to aquatic exposure and not to dietary exposure. These findings suggest that road dust may have considerable impact on benthic organisms at high salinity levels.

APA, Harvard, Vancouver, ISO, and other styles

19

Ando, Maru, Mio Tsuchiya, Shun Itai, Tomomi Murayama, Yuta Kurashina, Yun Jung Heo, and Hiroaki Onoe. "Janus Hydrogel Microbeads for Glucose Sensing with pH Calibration." Sensors 21, no. 14 (July 15, 2021): 4829. http://dx.doi.org/10.3390/s21144829.

Full text

Abstract:

We present fluorescent Janus hydrogel microbeads for continuous glucose sensing with pH calibration. The Janus hydrogel microbeads, that consist of fluorescent glucose and pH sensors, were fabricated with a UV-assisted centrifugal microfluidic device. The microbead can calibrate the pH values of its surroundings and enables accurate measurements of glucose within various pH conditions. As a proof of concept, we succeeded in obtaining the accurate value of glucose concentration in a body-fluid-like sample solution. We believe that our fluorescent microbeads, with pH calibration capability, could be applied to fully implantable sensors for continuous glucose monitoring.

APA, Harvard, Vancouver, ISO, and other styles

20

Pazoki, M., J. Oscarsson, L. Yang, B. W. Park, E. M. J. Johansson, H. Rensmo, A. Hagfeldt, and G. Boschloo. "Mesoporous TiO2 microbead electrodes for solid state dye-sensitized solar cells." RSC Adv. 4, no. 91 (2014): 50295–300. http://dx.doi.org/10.1039/c4ra10049b.

Full text

Abstract:

Mesoporous TiO₂ microbead films have been investigated as working electrode for solid state dye sensitized solar cells and 3.5% efficiency was achieved. Low trap density of microbead film leads to high voltage and fast electron transport.

APA, Harvard, Vancouver, ISO, and other styles

21

Yang, Yao-Tzu, Jhih-Cheng Wang, and Han-Sheng Chuang. "Developing Rapid Antimicrobial Susceptibility Testing for Motile/Non-Motile Bacteria Treated with Antibiotics Covering Five Bactericidal Mechanisms on the Basis of Bead-Based Optical Diffusometry." Biosensors 10, no. 11 (November 19, 2020): 181. http://dx.doi.org/10.3390/bios10110181.

Full text

Abstract:

Rapid antimicrobial susceptibility testing (AST) is an effective measure in the treatment of infections and the prevention of bacterial drug resistance. However, diverse antibiotic types and bacterial characteristics have formed complicated barriers to rapid diagnosis. To counteract these limitations, we investigated the interactions between antibiotic-treated bacteria and functionalized microbeads in optical diffusometry. The conjugation with bacteria increased the effective microbead complex size, thereby resulting in a temporal diffusivity change. The yielded data were sorted and analyzed to delineate a pattern for the prediction of antimicrobial susceptibility. The outcome showed that a completed rapid AST based on the trend of microbead diffusivity could provide results within 3 h (2 h measurement + 1 h computation). In this research, we studied four bacterial strains, including Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus, and six antibiotics. Despite the different inhibitory effects caused by various antibiotics, similar trends in diffusivity alteration for all susceptible and resistant cases in the last 40 min of the 2-h measurement period were deduced. In addition, the AST results obtained using optical diffusometry showed good agreement with those acquired from the commercial instrument and conventional culture methods. Finally, we conducted a single-blinded clinical test, and the sensitivity, specificity, and accuracy of the system reached 92.9%, 91.4%, and 91.8%, respectively. Overall, the developed optical diffusometry showcased rapid AST with a small sample volume (20 μL) and low initial bacterial count (105 CFU/mL). This technique provided a promising way to achieve early therapy against microbial diseases in the future.

APA, Harvard, Vancouver, ISO, and other styles

22

Abbah, S. A., W. W. Lu, S. L. Peng, D. M. K. Aladin, Z. Y. Li, W. K. Tam, K. M. C. Cheung, K. D. K. Luk, and Guang-Qian Zhou. "Extracellular Matrix Stability of Primary Mammalian Chondrocytes and Intervertebral Disc Cells Cultured in Alginate-Based Microbead Hydrogels." Cell Transplantation 17, no. 10-11 (October 2008): 1181–92. http://dx.doi.org/10.3727/096368908787236648.

Full text

Abstract:

Three-dimensional alginate constructs are widely used as carrier systems for transplantable cells. In the present study, we evaluated the chondrogenic matrix stability of primary rat chondrocytes and intervertebral disc (IVD) cells cultured in three different alginate-based microbead matrices to determine the influence of microenvironment on the cellular and metabolic behaviors of chondrogenic cells confined in alginate microbeads. Cells entrapped in calcium, strontium, or barium ion gelled microbeads were monitored with the live/dead dual fluorescent cell viability assay kit and the 1,9-dimethylmethylene blue (DMB) assay designed to evaluate sulfated glycosaminoglycan (s-GAG) production. Expression of chondrogenic extracellular matrix (ECM) synthesis was further evaluated by semiquantitative RT-PCR of sox9, type II collagen, and aggrecan mRNAs. Results indicate that Ca and Sr alginate maintained significantly higher population of living cells compared to Ba alginate (p < 0.05). Production of s-GAG was similarly higher in Ca and Sr alginate microbead cultures compared to Ba alginate microbeads. Although there was no significant difference between strontium and calcium up to day 14 of culture, Sr alginate showed remarkably improved cellular and metabolic activities on long-term cultures, with chondrocytes expressing as much as 31% and 44% greater s-GAG compared to calcium and barium constructs, respectively, while IVD cells expressed 63% and 74% greater s-GAG compared to calcium and barium constructs, respectively, on day 28. These findings indicate that Sr alginate represent a significant improvement over Ca- and Ba alginate microbeads for the maintenance of chondrogenic phenotype of primary chondrocytes and IVD cells.

APA, Harvard, Vancouver, ISO, and other styles

23

Bhattacharjee, Jashdeep, Barun Das, Alaknanda Mishra, Preeti Sahay, and Pramod Upadhyay. "Monocytes isolated by positive and negative magnetic sorting techniques show different molecular characteristics and immunophenotypic behaviour." F1000Research 6 (November 23, 2017): 2045. http://dx.doi.org/10.12688/f1000research.12802.1.

Full text

Abstract:

Background: Magnetic sorting of cells, based on microbead conjugated antibodies (Abs), employs positive as well as negative immunomagnetic separation methods, for isolation of a specific cell population. These microbeads are suggested to be nontoxic, biodegradable carriers conjugated to various antibodies. Isolation of cells through positive selection involves the attachment of antibody conjugated microbeads to the cells of interest, followed by their isolation in the presence of a strong magnetic field to obtain higher purity. Negative selection involves attachment of microbead conjugated antibodies to all other cell populations except the cells of interest, which remain untagged. In the present study, we compared the two methods for their effect on functional and immunophenotypic behavior of isolated CD14+ monocytes.Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from blood collected from healthy volunteers by density gradient centrifugation. Human blood derived monocytes were isolated through positive selection and negative selection, making use of the appropriate monocyte isolation kit. Monocytes were then stimulated with lipopolysaccharide (LPS) and their activation and proliferation capacity were examined. The degradation or dissociation of cell-bound microbeads was also investigated.Results: We observed an impaired LPS sensitivity as well as poor activation and proliferation capacity upon stimulation by LPS in positively sorted CD14+ monocytes as compared to negatively sorted CD14+ monocytes. The attached microbeads did not degrade and remained attached to the cells even after 6 days of culture.Conclusions: Our results suggest that positively sorted CD14+ cells exhibit hampered functionality and may result in inaccurate analysis and observations in downstream applications. However, these cells can be used for immediate analytical procedures.

APA, Harvard, Vancouver, ISO, and other styles

24

Bhattacharjee, Jashdeep, Barun Das, Alaknanda Mishra, Preeti Sahay, and Pramod Upadhyay. "Monocytes isolated by positive and negative magnetic sorting techniques show different molecular characteristics and immunophenotypic behaviour." F1000Research 6 (December 22, 2017): 2045. http://dx.doi.org/10.12688/f1000research.12802.2.

Full text

Abstract:

Background: Magnetic sorting of cells, based on microbead conjugated antibodies (Abs), employs positive as well as negative immunomagnetic separation methods, for isolation of a specific cell population. These microbeads are suggested to be nontoxic, biodegradable carriers conjugated to various antibodies. Isolation of cells through positive selection involves the attachment of antibody conjugated microbeads to the cells of interest, followed by their isolation in the presence of a strong magnetic field to obtain higher purity. Negative selection involves attachment of microbead conjugated antibodies to all other cell populations except the cells of interest, which remain untagged. In the present study, we compared the two methods for their effect on functional and immunophenotypic behavior of isolated CD14+ monocytes.Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from blood collected from healthy volunteers by density gradient centrifugation. Human blood derived monocytes were isolated through positive selection and negative selection, making use of the appropriate monocyte isolation kit. Monocytes were then stimulated with lipopolysaccharide (LPS) and their activation and proliferation capacity were examined. The degradation or dissociation of cell-bound microbeads was also investigated.Results: We observed an impaired LPS sensitivity as well as poor activation and proliferation capacity upon stimulation by LPS in positively sorted CD14+ monocytes as compared to negatively sorted CD14+ monocytes. The attached microbeads did not degrade and remained attached to the cells even after 6 days of culture.Conclusions: Our results suggest that positively sorted CD14+ cells exhibit hampered functionality and may result in inaccurate analysis and observations in downstream applications. However, these cells can be used for immediate analytical procedures.

APA, Harvard, Vancouver, ISO, and other styles

25

Bhattacharjee, Jashdeep, Barun Das, Alaknanda Mishra, Preeti Sahay, and Pramod Upadhyay. "Monocytes isolated by positive and negative magnetic sorting techniques show different molecular characteristics and immunophenotypic behaviour." F1000Research 6 (March 28, 2018): 2045. http://dx.doi.org/10.12688/f1000research.12802.3.

Full text

Abstract:

Background: Magnetic sorting of cells, based on microbead conjugated antibodies (Abs), employs positive as well as negative immunomagnetic separation methods, for isolation of a specific cell population. These microbeads are suggested to be nontoxic, biodegradable carriers conjugated to various antibodies. Isolation of cells through positive selection involves the attachment of antibody conjugated microbeads to the cells of interest, followed by their isolation in the presence of a strong magnetic field to obtain higher purity. Negative selection involves attachment of microbead conjugated antibodies to all other cell populations except the cells of interest, which remain untagged. In the present study, we compared the two methods for their effect on functional and immunophenotypic behavior of isolated CD14+ monocytes.Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from blood collected from healthy volunteers by density gradient centrifugation. Human blood derived monocytes were isolated through positive selection and negative selection, making use of the appropriate monocyte isolation kit. Monocytes were then stimulated with lipopolysaccharide (LPS) and their activation and proliferation capacity were examined. The degradation or dissociation of cell-bound microbeads was also investigated.Results: We observed an impaired LPS sensitivity as well as poor activation and proliferation capacity upon stimulation by LPS in positively sorted CD14+ monocytes as compared to negatively sorted CD14+ monocytes. The attached microbeads did not degrade and remained attached to the cells even after 6 days of culture.Conclusions: Our results suggest that positively sorted CD14+ cells exhibit hampered functionality and may result in inaccurate analysis and observations in downstream applications. However, these cells can be used for immediate analytical procedures.

APA, Harvard, Vancouver, ISO, and other styles

26

Sreenivasan, PK, VI Haraszthy, and JJ Zambon. "The effect of a microbead dentifrice on microbial load in oral microenvironments." International Journal of Dental Hygiene 9, no. 2 (April 12, 2011): 136–42. http://dx.doi.org/10.1111/j.1601-5037.2010.00465.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Ren, Zhi Min, Xi Nie, and Sheng Shu Ai. "Influence of Blocking Agents on Non-Specific Background of Polystyrene Microbeads in Serum Immunoassay." Advanced Materials Research 641-642 (January 2013): 858–61. http://dx.doi.org/10.4028/www.scientific.net/amr.641-642.858.

Full text

Abstract:

In this paper, we used bovine serum albumin and polymer as the blocking agents and investigated the effect of blocking agents on non-specific background of polystyrene microbead that used the human serum immunoassay.The results showed that the nonspecific background is lower by using polymer blocking agents. The best blocking condition was that microbeads were blocked by PVXT (0.5% polyvinyl alcohol PVA, 0.8% polyvinylpyrrolidone, 0.05% Tween-20, PBS phosphate buffer, pH7.0) for two hours at room temperature.

APA, Harvard, Vancouver, ISO, and other styles

28

Wang, Zhi. "Study on the Fabrication of Carbon Nanotube - Mesocarbon Microbead Composites." Advanced Materials Research 631-632 (January 2013): 486–89. http://dx.doi.org/10.4028/www.scientific.net/amr.631-632.486.

Full text

Abstract:

Carbon nanotube-Mesocarbon microbead composites were synthesized from coal tar pitch with carbon nanotubes (CNTs) as additive. The effect of CNTs addition and process parameters on the growth and morphologies of Mesocarbon microbeads (MCMBs) was investigated. The results show that adding CNTs enhances the nucleation and inhibits the growth and coalescence of MCMBs. Under the same thermal condensation conditions, the MCMBs made in the presence of CNTs tend to have smaller size, lower yield and more uniform size distribution, but more CNTs can lead to poor spherical degrees. Compared with the raw CNTs, the CNTs treated with blended acid can achieve better sphere and more uniform MCMBs with increasing CNTs ratio.

APA, Harvard, Vancouver, ISO, and other styles

29

Hoang, T. T. T., B. A. Nguyen, N. N. Q. Pham, N. B. Nguyen, T. K. T. Tran, T. C. L. Tu, T. D. Huynh, et al. "The potential emission of personal care products derived plastic microbeads: a case study of Ho Chi Minh City, Vietnam." IOP Conference Series: Earth and Environmental Science 964, no. 1 (January 1, 2022): 012013. http://dx.doi.org/10.1088/1755-1315/964/1/012013.

Full text

Abstract:

Abstract Plastic microbeads are commonly used in many personal care products and can cause adverse impacts to the environment and ecosystem. The toxicological problem with these pollutants are due to their non-biodegradable materials, which washed down the drain; end up accumulating in the aquatic system causing increased frequency and quantity of items ingested by biota. Several polymers (e.g. Polyethylene) especially those found in plastic microbeads have been reported to be in tandem with other toxic contaminants serving as a vector for their transports in the environment. Thus, the legislative ban for plastic microbeads is used in some developed countries, but many countries including Vietnam do not take any legal action. This present study aimed at potential microbead’s existence in the cosmetic market of Ho Chi Minh City (HCMC). The list and ingredients of microbeads containing personal care products (toothpaste, facial cleanser/scrubs and body wash/scrubs) have been checked. The microbeads containing PCPs are common for all explored categories, especially in toothpaste. Data from the online questionnaire survey have shown that 98% of respondents have frequently used at least one microbead containing product. Four polymers (Polyethylene, Acrylates Copolymer Styrene/Acrylates Copolymer and Acrylates/C10-30 Alkyl Acrylate Crosspolymer) have been observed in the product package of several facial cleansers and body scrubs. Thus, the potential negative impacts of this contaminant should not be ignored.

APA, Harvard, Vancouver, ISO, and other styles

30

Nedovic, Viktor, Verica Manojlovic, Ulf Pruesse, Branko Bugarski, Jasna Djonlagic, and Klaus Vorlop. "Optimization of the electrostatic droplet generation process for controlled microbead production: Single nozzle system." Chemical Industry and Chemical Engineering Quarterly 12, no. 1 (2006): 53–57. http://dx.doi.org/10.2298/ciceq0601053n.

Full text

Abstract:

The aim of this study was to optimize the electrostatic extrusion process for producing small, spherical and uniform microbeads with different fluid viscosities by varying the operating parameters in very wide ranges. Alginate was used as a model polymer. Since the rheological behavior of the solution is one of the parameters that affects the flow dynamics during extrusion, viscosity measurements of solutions with different alginate content were performed. The results obtained in this study show that an electrostatic droplet generator can be used for the production of spherical microbeads of narrow size distribution from low- and medium- viscous fluids (0.5, 1, and 2% of alginate). The average microbead diameter for low-viscous solutions was less than 100 micrometers. It was possible to obtain beads smaller than 500 micrometers that were very uniform (standard deviations less than 2.5%) and of spherical (the shape distortion was less than 1%) from medium-viscous alginate solution (2%). By reducing the polymer flowrate to less than 1 ml/h, even smaller microbeads were produced with diameters of about 300 micrometers. The particular contribution of this paper is in exceeding limitations regarding the use of high-viscous polymer solutions. Optimization of the operating conditions that included the use of a very small needle (0.15 mm), enlargement of the electrode distance to more than 20 cm and a severe reduction in the polymer flow rate to lower than 5 ml/h (for 3% alginate) or 1 ml/h (for 4% alginate) enabled the production of small, entirely spherical and uniform microbeads with an average microbead diameter lower than 500 and 700 micrometers in the case of 3 and 4% of alginate, respectively.

APA, Harvard, Vancouver, ISO, and other styles

31

Shim, Jae-Rin, Jung-Yeon Hwang, Jong-Soon Park, and Young-Mi Chung. "Process optimization of PLA microbead production." Journal of the Korea Academia-Industrial cooperation Society 23, no. 1 (January 31, 2022): 538–47. http://dx.doi.org/10.5762/kais.2022.23.1.538.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Waghmare, Prashant R., and Sushanta K. Mitra. "Contact Angle Hysteresis of Microbead Suspensions." Langmuir 26, no. 22 (November 16, 2010): 17082–89. http://dx.doi.org/10.1021/la1025526.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

Liu, Paul Peng, Karl Skucha, Yida Duan, Mischa Megens, Jungkyu Kim, Igor I. Izyumin, Simone Gambini, and Bernhard Boser. "Magnetic Relaxation Detector for Microbead Labels." IEEE Journal of Solid-State Circuits 47, no. 4 (April 2012): 1056–64. http://dx.doi.org/10.1109/jssc.2012.2185339.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Nunes Kirchner, Carolina, Markus Träuble, and Gunther Wittstock. "Diffusion and Reaction in Microbead Agglomerates." Analytical Chemistry 82, no. 7 (April 2010): 2626–35. http://dx.doi.org/10.1021/ac100168z.

Full text

APA, Harvard, Vancouver, ISO, and other styles

35

Thompson, Jason A., Xiaoguang Du, Joseph M. Grogan, Michael G. Schrlau, and Haim H. Bau. "Polymeric microbead arrays for microfluidic applications." Journal of Micromechanics and Microengineering 20, no. 11 (October 15, 2010): 115017. http://dx.doi.org/10.1088/0960-1317/20/11/115017.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Manesse, Mael, Aaron F. Phillips, Christopher N. LaFratta, Manuel A. Palacios, Ryan B. Hayman, and David R. Walt. "Dynamic microbead arrays for biosensing applications." Lab on a Chip 13, no. 11 (2013): 2153. http://dx.doi.org/10.1039/c3lc00044c.

Full text

APA, Harvard, Vancouver, ISO, and other styles

37

Li, Fei, Hong-jun Ni, Jun Wang, Bao-de Sun, and Zhao-hui Du. "Gelcasting of aqueous mesocarbon microbead suspension." Carbon 42, no. 14 (2004): 2989–95. http://dx.doi.org/10.1016/j.carbon.2004.07.012.

Full text

APA, Harvard, Vancouver, ISO, and other styles

38

Okunlola, A., and S. T. Oloye. "Influence of Pregelatinized Breadfruit Starch-Alginate Blend as a Sustained Release Polymer in Theophylline Microbeads Using Box Behnken Design." Nigerian Journal of Pharmaceutical Research 16, no. 2 (January 19, 2021): 143–51. http://dx.doi.org/10.4314/njpr.v16i2.5.

Full text

Abstract:

Background: Apart from the coating property of modified starches on drugs, these natural polymers also acts as release rate retardants.Objectives: To evaluate the potential of pregelatinized breadfruit (Artocarpus altilis) starch as a carrier in microbead formulations of theophylline using different blend combinations with sodium alginate and to determine the optimized formulation using Box-Behnken design.Method: Theophylline microbeads were prepared using the ionic gelation method. The 3 factor-3 level Box-Behnken design was employed for constructing polynomial models to optimize the microbeads, involving 3 independent variables (polymer type, X1, polymer: drug ratio, X2, and concentration of calcium chloride, X3) and 2 dependent variable (entrapment efficiency and quantity of drug released in 12 h, Q12).Results: Entrapment efficiency was 35 - 71 % while the values of Q12 was 38 - 88 %. The three variables, X1, X2 and X3, were positive for entrapment efficiency but negative for Q12, implying that increase from low to medium and then to high level resulted in an increase in entrapment but a decrease in Q12 (sustained release), both desirable effects. Factor X1 had the most significant influence on entrapment efficiency and Q12 (p = 0.002; p = 0.0001, respectively). The optimized formulation with starch:polymer 2:1, polymer:drug 3:1 and 7.5%w/v calcium chloride solution gave an entrapment efficiency 65% with Q12 of 38.75%.Conclusion: Pregelatinized breadfruit starch enhanced entrapment efficiency while retarding drug release, showing its potential as a polymer for sustained release in microbead formulations. Keywords: Box-Behnken design; Breadfruit starch; Ionic gelation, Microbeads, Pregelatinization

APA, Harvard, Vancouver, ISO, and other styles

39

Zhang, Jian, Edgar Abadia, Guislaine Refregier, Silva Tafaj, Maria Laura Boschiroli, Bertrand Guillard, Antoine Andremont, Raymond Ruimy, and Christophe Sola. "Mycobacterium tuberculosis complex CRISPR genotyping: improving efficiency, throughput and discriminative power of ‘spoligotyping’ with new spacers and a microbead-based hybridization assay." Journal of Medical Microbiology 59, no. 3 (March 1, 2010): 285–94. http://dx.doi.org/10.1099/jmm.0.016949-0.

Full text

Abstract:

The aims of the present study were to implement a microbead-based ‘spoligotyping’ technique and to evaluate improvements by the addition of a panel of 25 extra spacers that we expected to provide an increased resolution on principal genetic group 1 (PGG 1) strains. We confirmed the high sensitivity and reproducibility of the classical technique using the 43 spacer panel and we obtained perfect agreement between the membrane-based and the microbead-based techniques. We further demonstrated an increase in the discriminative power of an extended 68 spacer format for differentiation of PGG 1 clinical isolates, in particular for the East African–Indian clade. Finally, we define a limited yet highly informative reduced 10 spacer panel set which could offer a more cost-effective option for implementation in resource-limited countries and that could decrease the need for additional VNTR (variable number of tandem repeats) genotyping work in molecular epidemiological studies. We also present an economic analysis comparing membrane-based and microbead-based techniques.

APA, Harvard, Vancouver, ISO, and other styles

40

Spero, Richard Chasen, Rachel Sircar, Susan T. Lord, Alisa S. Wolberg, and Richard Superfine. "High Throughput Screening of Fibrin Clots: Transport and Mechanics Measured by Microbeads." Blood 112, no. 11 (November 16, 2008): 4095. http://dx.doi.org/10.1182/blood.v112.11.4095.4095.

Full text

Abstract:

Abstract Correlations have been shown between cardiovascular disease and the blood clot properties measured by turbidity, permeability, elasticity, and electron or confocal microscopy. With the exception of turbidity, these techniques are low throughput and usually require large sample volumes. We are developing clinically relevant, high throughput compatible measurements of fibrin structure and mechanics. These methods use microbeads, where particles ~0.1 – 10 um are embedded in clots. We have measured the local elastic modulus of fibrin clots using a high throughput compatible technology that magnetically applies force to microbeads. In a single microplate preparation, with clots of concentrations 0.5, 1, and 3 mg/mL, we measured the stiffness G = 5.8, 12, and 42 (+/− 2, 5, and 20) Pa. Using prior technology for elasticity measurements, this experiment would have required 14 separate specimen preparations. To quantify clot structure, we have measured microbead transport due to passive diffusion. PEG-coated beads passively diffused over long distances in fibrin gels and continued diffusing for >24 hours. As expected, increasing fibrinogen concentration suppressed passive transport (from >36 um at 1 mg/mL to <18 um at 3 mg/mL over 15 minutes), and, as particle size increased from 200 nm to 1 um, transport dropped to zero. However, larger PEG-coated beads (2.8 um) transported long distances (> 100 um) through these clots when driven by applied magnetic forces. We conclude that microbead techniques show promise as high throughput alternatives to measuring permeability and elasticity in research and clinical fibrin clots.

APA, Harvard, Vancouver, ISO, and other styles

41

Fritzler, Marvin J., and Mark L. Fritzler. "Microbead-based technologies in diagnostic autoantibody detection." Expert Opinion on Medical Diagnostics 3, no. 1 (December 23, 2008): 81–89. http://dx.doi.org/10.1517/17530050802651561.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Heemskerk, Bianca, Annelies Jorritsma, Raquel Gomez-Eerland, Mireille Toebes, John B. A. G. Haanen, and Ton N. M. Schumacher. "Microbead-Assisted Retroviral Transduction for Clinical Application." Human Gene Therapy 21, no. 10 (October 2010): 1335–42. http://dx.doi.org/10.1089/hum.2009.208.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Willmott, Geoff R., Matthew G. Fisk, and James Eldridge. "Magnetic microbead transport during resistive pulse sensing." Biomicrofluidics 7, no. 6 (November 2013): 064106. http://dx.doi.org/10.1063/1.4833075.

Full text

APA, Harvard, Vancouver, ISO, and other styles

44

Schieber, Jay D., and Ekaterina Pilyugina. "What is Measured By Passive Microbead Rheology?" Biophysical Journal 98, no. 3 (January 2010): 369a. http://dx.doi.org/10.1016/j.bpj.2009.12.1988.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Fardel, Romain, Yu-Cheng Tsai, and Craig B. Arnold. "Microbead dynamics in optical trap assisted nanopatterning." Applied Physics A 112, no. 1 (September 8, 2012): 23–28. http://dx.doi.org/10.1007/s00339-012-7200-3.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Ghionea, Simon, Pallavi Dhagat, and Albrecht Jander. "Ferromagnetic Resonance Detection for Magnetic Microbead Sensors." IEEE Sensors Journal 8, no. 6 (June 2008): 896–902. http://dx.doi.org/10.1109/jsen.2008.923904.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Jung, Jae Hwan, Byung Hyun Park, Young Ki Choi, and Tae Seok Seo. "A microbead-incorporated centrifugal sample pretreatment microdevice." Lab on a Chip 13, no. 17 (2013): 3383. http://dx.doi.org/10.1039/c3lc50266j.

Full text

APA, Harvard, Vancouver, ISO, and other styles

48

Pervushina, M. N., and R. R. Aliev. "Microbead cracking catalysts with improved strength properties." Chemistry and Technology of Fuels and Oils 33, no. 3 (May 1997): 138–40. http://dx.doi.org/10.1007/bf02767753.

Full text

APA, Harvard, Vancouver, ISO, and other styles

49

Moya, Monica L., Michael Morley, Omaditya Khanna, Emmanuel C. Opara, and Eric M. Brey. "Stability of alginate microbead properties in vitro." Journal of Materials Science: Materials in Medicine 23, no. 4 (February 16, 2012): 903–12. http://dx.doi.org/10.1007/s10856-012-4575-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

Yu, Linpo, Yang Liu, and Ming Zhou. "Improved electrochemiluminescence labels for heterogeneous microbead immunoassay." Analytical and Bioanalytical Chemistry 408, no. 25 (May 14, 2016): 7095–103. http://dx.doi.org/10.1007/s00216-016-9583-z.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!