Dissertations / Theses on the topic 'Mice Color'
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McGowan, Kelly Ann. "Genetics of skin color in mice /." May be available electronically:, 2008. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.
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Ruivenkamp, Claudia Antoinette Laetitia. "Colon cancer susceptibility genes in mice and humans." [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2003. http://dare.uva.nl/document/67685.
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Ahmed, F. A. W. "Pleiotropic effects of coat-colour mutants in mice." Thesis, University of Essex, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375647.
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Miyamoto, Shingo. "Suppressive effects of flavonoids on hyperleptinemia and colon carcinogenesis in mice." Kyoto University, 2009. http://hdl.handle.net/2433/126529.
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Kyoto University (京都大学)0048
新制・課程博士
博士(農学)
甲第14843号
農博第1783号
新制||農||974(附属図書館)
学位論文||H21||N4483(農学部図書室)
27249
UT51-2009-F485
京都大学大学院農学研究科食品生物科学専攻
(主査)教授 入江 一浩, 教授 伏木 亨, 教授 河田 照雄
学位規則第4条第1項該当
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Richter, Cornelia, San Juan Martina Herrero, Benno Weigmann, Dominik Bergis, Katrin Dauber, Michael H. Muder, Gustavo B. Baretton, et al. "Defective IL-23/IL-17 Axis Protects p47phox−/− Mice from Colon Cancer." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-226852.
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In the colon, a sophisticated balance between immune reaction and tolerance is absolutely required. Dysfunction may lead to pathologic phenotypes ranging from chronic inflammatory processes to cancer development. Two prominent modulators of colon inflammation are represented by the closely related cytokines interleukin (IL)-12 and IL-23, which initiate adaptive Th1 and Th17 immune responses, respectively. In this study, we investigated the impact of the NADPH oxidase protein p47phox, which negatively regulates IL-12 in dendritic cells, on colon cancer development in a colitis-associated colon cancer model. Initially, we found that IL-12−/− mice developed less severe colitis but are highly susceptible to colon cancer. By contrast, p47phox−/− mice showed lower tumor scores and fewer high grade tumors than wild-type (WT) littermates. Treatment with toll-like receptor 9 ligand CpG2216 significantly enhanced colitis in p47phox−/− mice, whereas tumor growth was simultaneously reduced. In tumor tissue of p47phox−/− mice, the IL-23/IL-17 axis was crucially hampered. IL-23p19 protein expression in tumor tissue correlated with tumor stage. Reconstitution of WT mice with IL-23p19−/− bone marrow protected these mice from colon cancer, whereas transplantation of WT hematopoiesis into IL-23p19−/− mice increased the susceptibility to tumor growth. Our study strengthens the divergent role of IL-12 and IL-23 in colon cancer development. With the characterization of p47phox as a novel modulator of both cytokines our investigation introduces a promising new target for antitumor strategies.
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Gillen, Aric. "The Effects of Electrochemical Therapy on Colon-25 Tumors in Balb-C Mice." Thesis, University of North Texas, 2000. https://digital.library.unt.edu/ark:/67531/metadc2720/.
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The purpose of the research was to treat immunodeficient mice, implanted with colon-25 tumors, with continuous and interrupted electrochemical therapy (ECT). ECT involves the placement of two electrodes, an anode near the center of the tumor and a cathode into the tumor periphery. A constant voltage is applied across the electrodes for a given period of time. The data showed that the interrupted and continuous ECT resulted in a decrease in mean tumor growth as compared to that of the sham controls. The histology of both ECT groups showed an increase presence of large vacuoles, randomly distributed tumor cells as well as the presence of "crevicing" in the medullary tissue. The differential leukocyte counts showed a distinct neutrophilia and lymphopenia in all groups at day 20 post tumor implantation. The results from the experimental groups appeared to support the findings of previous investigators.
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O'Connor, Christiane C. "The influence of lipids on the growth, development, and metastatic potential of transplantable colon tumor CT-26 in Balb/c mice." Thesis, Boston University, 1987. https://hdl.handle.net/2144/38089.
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Thesis (M.A.)--Boston UniversityPLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.
Evidence that dietary fats influence carcinogenesis comes from both epidemiological and experimental data. Previous experimental studies suggest that dietary fat acts as a promoter in chemically induced carcinogenesis and this effect depends on the degree of saturation and concentration of dietary fat. [TRUNCATED]
2031-01-01
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Edwards, Cheri Paris. "Your Blues Ain't Like Mine: Voices from the Other Side of the Color Line." Thesis, University of North Texas, 2019. https://digital.library.unt.edu/ark:/67531/metadc1609132/.
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This dissertation examines intra-racial colorism in works by writers who began their careers during the Harlem Renaissance, but whose writings span almost a century. In these writings, colorism; which can be defined as a bias directed toward an individual that is based on skin tone, is portrayed an intra-racial practice that results from the internalization of racist ideals. The practice relies on a hierarchy that most often privileges those closest to the color line. However, these depictions also show that the preponderance of skin tones can sometimes determine who is targeted. For the purposes of this study it is called reverse colorism when the bias is directed by individuals darker in skin tone toward those who are lighter. Consequently, the careful descriptions of the shades and hues of black characters becomes more than aesthetics and can be seen as a coded reference to experiential differences. While Alain Locke hailed the start of the Harlem Renaissance to signal the rise of The New Negro, the writings featured by female writers in this dissertation advance a less optimistic reality for women, who had to contend with both inter- and intra-racial bias because of their skin tone. Colorism is identified as a particularly prevalent presence in the lives of black women, who also saw skin tone subjectively and viewed themselves as darker than their male counterparts.
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Hayashi, Adam. "Effects of Daily Oral Injections of Quercetin on Implanted Colon-25 Tumor Growth in Balb-C Mice." Thesis, University of North Texas, 2000. https://digital.library.unt.edu/ark:/67531/metadc2525/.
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The effects of three oral dosages (0.4 mg, 0.8 mg, and 1.6 mg) of quercetin on Colon-25 tumors implanted in Balb-c mice were studied. The data in this study show that: (1) certain dosages of quercetin in alcohol solutions, reduces the weight, and size of implanted Colon-25 tumors in Balb-c mice, (2) these same dosages of quercetin all produce a profound neutrophilia combined with a significant lymphopenia at day 20 post-implantation, and (3) there was relatively little evidence of histological changes in the quercetin-treated tumor section which would indicate that the action(s) of quercetin is primarily at the subcellular level probably within the nuclei of the tumor cells.
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廖兆霖 and Shiu-lam Edgar Liu. "Pathogenic mechanisms of cigarette smoking on ulcerative colitis-associated neoplasia in mice." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B3124452X.
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Roubos, Sophia. "The Influence of Exercise During Weight Loss on Muscle Remodeling During Colon Cancer Induction In Mice." Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/38079.
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Background: Diet and exercise have been recommended to reduce the risk of colorectal cancer (CRC) in individuals with obesity. However, the effects of these interventions on muscle remodeling during CRC initiation in individuals who were previously obese is unknown. Since CRC is associated with a high-risk of cachexia, it is important to understand how diet and exercise interventions can impact muscle remodeling in populations at risk of developing CRC-induced cachexia. Our aim was to investigate the effects of weight loss, with or without exercise, on markers of muscle remodeling in a mouse model of CRC. We hypothesized that exercise plus weight loss would increase muscle mass, reduce muscle fibro/fatty tissue, and increase muscle stem/progenitor cell content compared to weight loss alone.
Methods: Mice consumed a high-fat diet (HFD) to induce obesity or a control (CON) diet. Subsequently, mice received injections of azoxymethane (AOM) to induce CRC. Then, weight loss was induced in HFD mice by placing them on the CON diet and those mice either remained sedentary (HFD-SED) or completed a treadmill exercise intervention (HFD-EX).
Results: After 40 weeks, mice were sacrificed and analyzed for markers of muscle remodeling. HFD-SED and HFD-EX showed weight loss and a loss in percent fat mass when looking at changes between sacrifice and before AOM injections (p<0.05 vs. CON). HFD-SED and HFD-EX had increased lean mass (p<0.05 vs. CON), and HFD-EX had increased tibialis anterior (TA) weight (p<0.05 vs. CON). The proportion of medium-sized fibers increased (p<0.05 vs. HFD-SED and CON) in HFD-EX, but there were no differences in overall cross-sectional area, myonuclei per fiber, or myonuclear domain. HFD-SED had increased fibrosis (p<0.05 vs. HFD-EX and CON) and adiposity (p<0.05 vs. CON). The number of committed (Pax7+MyoD+) satellite cells (SCs) and FAPs was greater in HFD-EX (p<0.05 vs. CON). There were no differences in uncommitted (Pax7+MyoD-) or differentiated (Pax7-MyoD+) SCs. Additionally, nuclear p-NF-κB was reduced following exercise (p<0.05), specifically in the interstitium with a significant decrease in the number of interstitial p-NF-κB cells in the HFD-EX group (p<0.05 vs. CON and HFD-SED).
Conclusions: Findings suggest that a HFD, followed by weight loss with exercise, can reduce fibrotic and fatty degeneration of the muscle and improve markers of muscle remodeling. These findings provide the rationale to further examine exercise interventions for maintaining muscle quality during weight loss interventions to reduce CRC-induced cachexia.
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Mazzei, Joseph Cayetano. "Suppression of intestinal inflammation and inflammation-driven colon cancer in mice by dietary sphingomyelin: Importance of peroxisome proliferator-activated receptor γ expression." Thesis, Virginia Tech, 2012. http://hdl.handle.net/10919/43766.
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Sphingolipid metabolites play a role in the initiation and perpetuation of inflammatory responses. Since intestinal inflammation is a driving force in the development of colon cancer, in the present study, we investigated the suppression of dextran sodium sulfate (DSS)-induced colitis by dietary sphingomyelin in mice that lack functional peroxisome proliferator-activated receptor γ (PPAR-γ) in intestinal epithelial and immune cells. Dietary spingomyelin decreased colonic inflammation in mice of both genotypes but more efficiently in mice expressing PPAR-γ. Using a real-time polymerase chain reaction array, we detected an up-regulation in genes involved in Th1 (interferon γ) and Th17 (interleukin [IL]-17 and IL-23) responses despite the reduced inflammation. However, the genes involved in Th2 (IL-4, IL-13 and IL-13ra2) and Treg (IL-10rb) anti-inflammatory responses were up-regulated in a PPAR-γ-dependent manner. In order to direct mechanistic studies of how PPAR-γ expression is involved in SM-induced suppression of DSS colitis, we investigated the effect of dietary SM in DSS-treated mice that lack PPAR-γ in the CD4+ T-cells. While the pathogenesis of colitis was independent of PPAR-γ expression in CD4+ T-cells, dietary SM decreased disease activity and colonic inflammation in mice of both genotypes but more efficiently in mice expressing PPAR-γ, indicating both PPAR-γ dependent and independent signaling pathways. In conclusion, in contrast to endogenous sphingolipid metabolites, dietary SM modulated both pro- and anti-inflammatory responses at the early stages of the disease in a partially PPAR-γ dependent manner resulting in a suppression of inflammation that may be critical for the suppression of inflammation-driven colon cancer.Master of Science
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Molnar, Valerie Anne. "If Your Love Were A Grain Of Sand Mine Would Be A Universe Of Beaches." VCU Scholars Compass, 2008. http://scholarscompass.vcu.edu/etd/685.
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Each stitch is a piece of me that I give, a moment of my life and a unit of my love, meticulously culminated into a universal visual language. The optimist in me knits for the cause, while my formalist counterpart works to make the images that sell my thoughts. I knit for the lovers.I make these objects as a practice and a confirmation of my optimism. I make these images to communicate and persuade as a serious contender while at the same time retaining my own optimism and sanity by promising to never take myself too seriously.
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Nakanishi, Yasutaka. "Regulatory T cells with superior immunosuppressive capacity emigrate from the inflamed colon to draining lymph nodes." Kyoto University, 2018. http://hdl.handle.net/2433/235982.
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Pampaloni, Amanda Carolina Montevechi. "Avaliação do efeito quimiopreventivo do óleo de pequi (Caryocar brasiliense Camb.) em cólon de camundongo Balb/C." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/5/5160/tde-24032016-141530/.
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A flora brasileira possui plantas com grande potencial de investigação na carcinogênese humana, sendo uma delas o Pequi (Caryocar brasiliense Camb.). Esta fruta da região central do Brasil contém na sua polpa e principalmente no extrato do óleo da sua polpa, várias substâncias antioxidantes, as quais estão relacionadas com a redução do risco de doenças degenerativas. Neste estudo propomos avaliar o potencial quimiopreventivo do C. brasiliense contra mecanismos pré-neoplásicos no cólon induzidas quimicamente pelo azoximetano (AMO) em camundongos. O iniciador AMO na concentração 10mg/kg foi injetado por injeção subcutânea em camundongos de 40 dias de idade. Foram formados quatro grupos experimentais: C (controle sem tratamento); AMO (azoximetano 10mg/kg), PQ400 (óleo de pequi 400mg/kg) e OMAPQ400 (OMA+ 400mg/kg de óleo de pequi). Estes dois últimos grupos receberam o óleo de pequi a partir do 40º dia até o 110° dia de vida. A altura das criptas intestinais analisadas, não revelou diferença em nenhum dos grupos. O óleo de C. brasiliense reduziu em 18,7% a proliferação celular no grupo OMAPQ400 quando comparado com os grupos C e AMO. Houve aumento da produção de muco neutro no grupo AMO quando comparado com o C e diminuição no grupo AMOPQ400. Houve hipermetilação do DNA no grupo AMO, e hipometilação no grupo AMOPQ400, sendo estes iguais ao grupo controle (C). A expressão do gene oncogênico (c-Myc) diminuiu no grupo tratado com óleo de pequi em comparação com o grupo tratado com o iniciador AMO. Todos esses efeitos podem ser devidos à presença de antioxidantes na polpa do óleo. Por isso, concluímos que o óleo de C. brasiliense possui efeito protetor contra alguns mecanismos precursores de lesões pré-neoplásicas do cólonThe Brazilian native flora has several plants which have thigh research potential, among those the Pequi (Caryocar brasiliense Camb.). This fruit from the central region of Brazil contains several antioxidant substances in its pulp and mainly in its pulp oil extract, which are related with the reduction of the risk of degenerative diseases. In this study we propose to evaluate the chemopreventive potential of C. brasiliense against neoplastic mechanisms in the colon induced chemically by azoximetano (AMO) in mice. AMO 10mgkg concentration initiator was subcutaneous injection in mice of 40 days of age. Four experimental groups were formed: C (untreated control); AMO (azoximetano 10mg/kg), PQ400 (pequi oil 400mgkg) and AMOPQ400 (AMO 400mgkg pequi oil). These last two groups received the pequi. The height of the intestinal crypts analyzed revealed no difference among groups. The oil of C. brasiliense reduced the cell proliferation by 18.7% in AMOPQ400 group when compared with the groups C and AMO. There was increased production of neutral mucus in the group AMO when compared with the C group and decrease in the AMOPQ400 group. The pequi oil induced DNA hipomethylation in the AMOPQ400 group and hipermethylation in AMO group. The expression of the oncogenic gene c-Myc decreased in the group treated with pequi oil compared to the group treated with the initiator AMO alone. All these effects can be due to the presence of antioxidants in pulp oil. Therefore, we conclude that the oil of C. brasiliense has protective effect against some mechanisms that precede of neoplastic lesions of the colon
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Janick, Christophe. "Détection de passage sur fond structure coloré : mise en coopération des informations multispectrales." Paris 6, 2000. http://www.theses.fr/2000PA066228.
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Nowak, Johannes [Verfasser]. "Colitis-associated colon tumorigenesis is suppressed in transgenic mice rich in endogenous n-3 fatty acids / Johannes Nowak." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2010. http://d-nb.info/1024865533/34.
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Owyang, Angela. "The Influence of Cinematic Elements in Pierre Jodlowski's Works Based on Colour." Diss., The University of Arizona, 2014. http://hdl.handle.net/10150/314684.
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Pierre Jodlowski's Works Based on Colour consist of three pieces: Série Noire (2005), Série Blanche (2007), and Série Rose (2012) pour piano et dispositif électroacoustique. According to the composer, "each colour provides an opportunity to investigate a particular link of music with imagery." The pieces serve as a musical representation of cinematic elements through the synchronization of music and sound effects. The musical language of the cycle draws influences from various sources including the music of expressionism, musique concrète, as well as standard soundtrack scoring. The intermingling of various melodies, harmonies, and rhythms creates a synthesis between the piano and the electroacoustic soundtrack (tape).In Pierre Jodlowski's Works Based on Colour, he seeks to form an interdisciplinary collaboration between music and visual multimedia. The composer's interest in cinematography and mental processes is evident through his selective mixing of audio effects, sounds (real sounds versus synthesized sounds), and piano. According to Jodlowski the visual aspects, or mise-en-scène, of cinema should surface in the minds of the listener after hearing each of the works. Jodlowski's method of mixing involves several techniques: the insertion of fragments of dialogue from various films, tinkering with inanimate objects, pre-recorded sounds of nature, and the distortion of sonic effects and instrumentation. Aside from the use of color as a symbol, Jodlowski's cycle contains extra-musical ideas that cover a wide range of cinematic themes, including atmospheric elements of film noir, the gradual shifts and changes in our mental processes, as well as love discourse and sex from erotic cinema and pornography. Jodlowski's Works Based on Colour stirs the listener's imagination through the clear delineation of contrasting sections, the dialogue-like interaction between the tape and the piano, and the inclusion of dialogue and sound effects from various films. A performer should have knowledge of cinematic elements, understand the purpose of cinematic concepts and their significance for a cinematic viewer, and recognize their function in Jodlowski's works before performing the Works Based on Colour.
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Gensollen, Thomas. "Rôle de la pantéthéinase épithéliale Vanin-1 dans l'inflammation intestinale." Thesis, Aix-Marseille, 2014. http://www.theses.fr/2014AIXM4032.
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Les cellules épithéliales produisent des facteurs influençant la réponse inflammatoire intestinale. L'étude de la contribution de ces facteurs à l'homéostasie des tissus est devenue essentielle à la compréhension des maladies inflammatoires chroniques intestinales (MICIs). Vanin-1 est une enzyme épithéliale qui produit des facteurs solubles dans les tissus dont l'intestin en réponse à un stress. Nous avons identifié Vanin-1 comme facteur de susceptibilité et marqueur des MICIs chez l'homme. La création d'une souris mutante nous a permis d'identifier des mécanismes d'action potentiels de Vanin-1 dans les tissus. Vanin-1 pourrait un jour être exploité pour développer une stratégie thérapeutique contre les MICIsIntestinal epithelial cells have recently been identified as producing factors influencing grandly the curse of intestinal inflammation. It became of a great importance, therefore, to identify the contribution of epithelial molecules to inflammatory bowel diseases (IBD). Vanin-1 is an epithelial enzymes which release soluble factors in tissues such as gut. This study identifies Vanin-1 as a marker and a susceptibility factor of IBD. By producing a mutant mice, we identified potential mechanisms by which Vanin-1 could act in tissues. It is hoped that Vnn1 can be exploited to develop future therapeutic strategies against IBD
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Boulard, Annick. "Stratégies de dépistage de masse des tumeurs colo-rectales : mise au point d'un modèle mathématique." Bordeaux 2, 1989. http://www.theses.fr/1989BOR23033.
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Delfour, Christophe. "Mise au point d'un protocole d'analyse du cycle par méthode tri paramétrique en cytométrie de flux : application au cancer du côlon." Montpellier 1, 2000. http://www.theses.fr/2000MON11111.
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Piau, Jean-Philippe. "Mise en evidence de deux genes codant pour des alpha (1,2) fucosyltransferases differentiellement exprimees dans des cellules coliques de rat : implication d'un de ces genes dans la tumorigenicite." Nantes, 1994. http://www.theses.fr/1994NANT04VS.
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Chido, Chakanya. "Fatty acid composition, colour stability and lipid oxidation of mince produced from fresh and frozen/thawed fallow deer meat." Thesis, University of Fort Hare, 2016. http://hdl.handle.net/10353/2479.
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The aim of the study was to determine the fatty acid composition, colour stability and lipid oxidation of fresh mince produced from fallow deer and to evaluate the effect of frozen storage duration on the retail display shelf life of the mince. A total of 31 fallow deer carcasses were used in the study. After cooling for 24hrs, the carcasses were deboned, external fat from the fore and hindquarter muscles removed and individually vacuum packed. For the first trial, seven fallow deer carcasses were used. Meat from the hind and fore-quarters of each carcass was divided into two equal batches per animal. One batch was minced (through a 5 mm die) and packed into oxygen permeable overwraps and refrigerated at 4°C for a period of eight days under retail display conditions. The second batch was vacuum packed and frozen at -20°C for 2 months at the end of which mince was also produced and monitored over an eight-day period under the same conditions that were used for the fresh mince. Colour, pH, lipid and myoglobin stability was determined. Proximate and fatty acid composition was also determined. No differences (P>0.05) were noted between proximate composition of fresh and frozen/thawed minced meat. The lipid content of fallow deer was 2.4 percent (±0.04). Total n3 fatty acids differed (P<0.05) between treatments and decreased with increased storage and display day. There were significant (P<0.05) treatment and time interactions on all measured colour parameters, TBARS and myoglobin forms. Fresh mince was lighter and had higher redness (a*) and yellowness (b*) values than mince from two months frozen stored meat. Hue angle for fresh mince remained stable throughout display whereas it increased for frozen/thawed mince. Fresh mince had lower TBARS values than frozen/thawed mince. Minced meat produced from frozen/thawed deer meat had higher surface met-myoglobin and total met-myoglobin percentages. Surface and total oxy-myoglobin percentage was higher in fresh mince. The first trial clearly showed colour and lipid stability differences between fresh mince and mince from frozen/thawed meat. It also showed that fresh mince has a longer retail display life than mince produced from frozen/thawed meat (six days and four days, respectively). In the second trial, the effects of frozen storage duration on colour and lipid stability were investigated. Twenty-four fallow deer were used. Twelve were harvested in June (6male 6female) and the other twelve in August (6 male 6female) of the same year.Twenty four hours after harvesting, the fore and hindquarter muscles of the carcasses were deboned, vacuum packed and kept at -20°C until October (i.e. 2months and 4months frozen storage period). Upon thawing, the meat was processed into mince following the same procedure used for the first trialand displayed for a fiveday period under retail display conditions. Frozen duration and gender had no effect (P>0.05) on the proximate composition of fallow deer meat. The total amount of saturated fatty acids (SFA) increased and total amount of poly unsaturated fatty acids (PUFA) decreased as frozen duration and display day increased (P<0.05). Frozen duration affected (P<0.01) lipid oxidation and percentage oxy-myoglobin. Mince pH and all colour parameters (L*, a*, b*,hue and chroma) differed (P<0.05) between treatments on day zero and three. Display day was a significant factor (P<0.05) on all measured parameters. By day three all parameters except pH showed signs of extended oxidation and discolouration as evidenced by reduced redness, decreased colour intensity and high TBARS values. This study showed that prolonged frozen storage negatively affects the colour and lipid stability of meat and increases oxidation of PUFAs during frozen storage. However, the study also suggests that although frozen/thawed meat has a shorter retail display shelf life; the proximate composition of the meat remains unchanged.
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Yan, Min. "15-HYDROXYPROSTAGLANDIN DEHYDROGENASE IS A TGF-beta INDUCED SUPPRESSOR OF HUMAN COLORECTAL CANCER." Connect to text online, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=case1100464322.
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Thesis (Ph. D.)--Case Western Reserve University, 2005.[School of Medicine] Department of Molecular Biology and Microbiology. Includes bibliographical references. Available online via OhioLINK's ETD Center.
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Setkov, Aleksandr. "IVORA (Image and Computer Vision for Augmented Reality) : Color invariance and correspondences for the definition of a camera/video-projector system." Thesis, Université Paris-Saclay (ComUE), 2015. http://www.theses.fr/2015SACLS168/document.
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La Réalité Augmentée Spatiale (SAR) vise à superposer spatialement l'information virtuelle sur des objets physiques. Au cours des dernières décennies ce domaine a connu une grande expansion et est utilisé dans divers domaines, tels que la médecine, le prototypage, le divertissement etc. Cependant, pour obtenir des projections de bonne qualité, on doit résoudre plusieurs problèmes, dont les plus importants sont la gamme de couleurs réduite du projecteur, la lumière ambiante, la couleur du fond, et la configuration arbitraire de la surface de projection dans la scène. Ces facteurs entraînent des distorsions dans les images qui requièrent des processus de compensation complémentaires.Les projections intelligentes (smart projections) sont au cœur des applications de SAR. Composées d'un dispositif de projection et d'un dispositif d'acquisition, elles contrôlent l'aspect de la projection et effectuent des corrections à la volée pour compenser les distorsions. Bien que les méthodes actives de Lumière Structurée aient été utilisées classiquement pour résoudre ces problèmes de compensation géométrique, cette thèse propose une nouvelle approche non intrusive pour la compensation géométrique de plusieurs surfaces planes et pour la reconnaissance des objets en SAR s'appuyant uniquement sur la capture du contenu projeté.Premièrement, cette thèse étude l'usage de l'invariance couleur pour améliorer la qualité de la mise en correspondance entre primitives dans une configuration d'acquisition des images vidéoprojetées. Nous comparons la performance de la plupart des méthodes de l'état de l'art avec celle du descripteur proposé basé sur l'égalisation d'histogramme. Deuxièmement, pour mieux traiter les conditions standard des systèmes projecteur-caméra, deux ensembles de données de captures de projections réelles, ont été spécialement préparés à des fins expérimentales. La performance de tous les algorithmes considérés est analysée de façon approfondie et des propositions de recommandations sont faites sur le choix des algorithmes les mieux adaptés en fonction des conditions expérimentales (paramètres image, disposition spatiale, couleur du fond...). Troisièmement, nous considérons le problème d'ajustement multi-surface pour compenser des distorsions d'homographie dans les images acquises. Une combinaison de mise en correspondance entre les primitives et de Flux Optique est proposée afin d'obtenir une compensation géométrique plus rapide. Quatrièmement, une nouvelle application en reconnaissance d'objet à partir de captures d'images vidéo-projetées est mise en œuvre. Finalement, une implémentation GPU temps réel des algorithmes considérés ouvre des pistes pour la compensation géométrique non intrusive en SAR basée sur la mise en correspondances entre primitivesSpatial Augmented Reality (SAR) aims at spatially superposing virtual information on real-world objects. Over the last decades, it has gained a lot of success and been used in manifold applications in various domains, such as medicine, prototyping, entertainment etc. However, to obtain projections of a good quality one has to deal with multiple problems, among them the most important are the limited projector output gamut, ambient illumination, color background, and arbitrary geometric surface configurations of the projection scene. These factors result in image distortions which require additional compensation steps.Smart-projections are at the core of PAR applications. Equipped with a projection and acquisitions devices, they control the projection appearance and introduce corrections on the fly to compensate distortions. Although active structured-light techniques have been so far the de-facto method to address such problems, this PhD thesis addresses a relatively new unintrusive content-based approach for geometric compensation of multiple planar surfaces and for object recognition in SAR.Firstly, this thesis investigates the use of color-invariance for feature matching quality enhancement in projection-acquisition scenarios. The performance of most state-of-the art methods are studied along with the proposed local histogram equalization-based descriptor. Secondly, to better address the typical conditions encountered when using a projector-camera system, two datasets of real-world projections were specially prepared for experimental purposes. Through a series of evaluation frameworks, the performance of all considered algorithms is thoroughly analyzed, providing several inferences on that which algorithms are more appropriate in each condition. Thirdly, this PhD work addresses the problem of multiple-surface fitting used to compensate different homography distortions in acquired images. A combination of feature matching and Optical Flow tracking is proposed in order to achieve a more low-weight geometric compensation. Fourthly, an example of new application to object recognition from acquired projections is showed. Finally, a real-time implementation of considered methods on GPU shows prospects for the unintrusive feature matching-based geometric compensation in SAR applications
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Fillmann, L?cio Sarubbi. "A express?o do fator tecidual no adenocarcinoma colo-retal : rela??o com angiog?nese e aspectos cl?nico-patol?gicos." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2006. http://tede2.pucrs.br/tede2/handle/tede/1530.
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Previous issue date: 2006-06-07A import?ncia da intera??o entre o mecanismo de coagula??o e o c?ncer ? reconhecida desde as descri??es pioneiras de Trousseau. Mais recentemente, estudos demonstraram a import?ncia do fator tecidual, uma prote?na de membrana celular ligada ao desencadeamento da cascata da coagula??o, na progress?o de neoplasias malignas atrav?s da estimula??o do processo de angiog?nese. Realizamos marca??o imunoistoqu?mica para o fator tecidual em 43 adenocarcinomas colo-retais de pacientes submetidos a tratamento cir?rgico no Servi?o de Coloproctologia do HSL-PUCRS e comparamos a intensidade da sua express?o com a densidade microvascular, crit?rios de estadiamento, idade, sexo e sobrevida geral. Oitenta e tr?s por cento dos tumores apresentaram alta express?o do fator tecidual, havendo uma rela??o estatisticamente significativa entre esta e a maior densidade microvascular (p=0,02). Observamos tamb?m que os pacientes com alta express?o do fator tecidual apresentavam uma m?dia de idade significativamente maior do que a dos pacientes com baixa express?o desta prote?na (p<0,01). Em conclus?o, a elevada intensidade de marca??o imunoistoqu?mica para o fator tecidual se relacionou com uma m?dia de densidade microvascular mais elevada e com pacientes mais idosos quando comparados aos casos com baixa express?o desta prote?na.
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Lemaire, Valérie. "Adénocarcinome du côlon : mise en évidence par RT-PCR de la dissémination hématogène de cellules coliques au stade métastatique." Paris 5, 1995. http://www.theses.fr/1995PA05P272.
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Snow, Dallin R. "Dietary Milk Fat Globule Membrane Reduces the Incidence of Aberrant Crypt Foci in Fischer-344 Rats and Provides Protections Against Gastrointestinal Stress in Mice Treated with Lipopolysaccharide." DigitalCommons@USU, 2010. https://digitalcommons.usu.edu/etd/831.
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Milk fat globule membrane surrounds the fat droplets of milk. It is a biopolymer containing primarily membrane glycoproteins and polar lipids which contribute to its properties as a possible neutraceutical. The aims of the studies were to determine if dietary milk fat globule membrane: (1) confers protection against colon carcinogenesis; and (2) promotes gut mucosal integrity while decreasing inflammation compared to diets containing corn oil or anhydrous milk fat.
Aim 1. Three dietary treatments differing only in the fat source were formulated: (1) AIN-76A, corn oil; (2) AIN-76A, anhydrous milk fat; and (3) AIN-76A, 50% milk fat globule membrane, 50% anhydrous milk fat. Each diet was formulated to contain 50 g/kg diet of fat and to be identical in macro and micro nutrient content. To assess protection against colon carcinogenesis, male, weanling Fischer-344 rats were randomly assigned to one of the three dietary treatments. Animals were injected with 1,2-dimethylhydrazine once per week at weeks 3 and 4. After 13 weeks animals were sacrificed, colons were removed, and aberrant crypt foci were counted by microscopy.
Rats fed the milk fat globule membrane diet (n = 16) had significantly fewer aberrant crypt foci (20.9 ± 5.7) compared to rats fed corn oil (n = 17) or anhydrous milk fat (n = 16) diets (31.3 ± 9.5 and 29.8 ± 11.4 respectively; P < 0.05).
Aim 2. Male BALB/c mice were randomly assigned to one of two diets: AIN- 76A, corn oil or AIN-76A, 50% milk fat globule membrane, 50% anhydrous milk fat. After 5 weeks mice were injected with saline vehicle control or lipopolysaccharide and gavaged with dextran-FITC. To assess gut mucosal integrity and inflammation, serum samples were assayed for dextran-FITC 24 and 48 hours after gavage, and a panel of 16 cytokine concentrations was analyzed.
Serum concentrations of IL-6, IL-10, IL-17, MCP-1, IFNγ, and TNFα decreased and gut permeability decreased 45% in lipopolysaccharide challenged mice fed milk fat globule membrane diet compared to control diet at 24 hours (P < 0.05).
Overall, the results of these aims suggest that diets containing milk fat globule membrane are protective against colon carcinogenesis, inhibit the inflammatory response, and protect against gastrointestinal stress.
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Althoff, Juliana Lorenzoni. "Express?o do fator de transcri??o da fam?lia ETS : PDEF no c?ncer colo-retal atrav?s de imunohistoqu?mica." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2009. http://tede2.pucrs.br/tede2/handle/tede/8332.
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Background: The colon-rectal cancer is the third most frequent neoplasia in the western world with the highest mortality rates. The progression of normal colon tissue to invasiveness neoplasia is follow by several processes called carcinogenesis. Afterwards it converges to the cellular migration and metastasis of the tissue. The metabolic ways of the E26 (Ets) transcription factors, identified in a great variety of species, contributes in this process, by activating or repressing the DNA transcription. Specially, the prostate-derived Ets factor (PDEF) which prognosis and cancer colon-rectal relation action is not completely elucidating at the moment. Methods: A retrospective cohort of patients with pathologic stage I ? III colon-rectal cancer, diagnosed and treated in the same institution between 2002 and 2008, was study. Histological and clinical features as well as clinical outcomes and survival were reviewed. The tissue microarrays (TMA), immunohistochemical analysis and image capture quantification were carried out in representative blocks of tumor with antibodies for the detection of the PDEF expression and Ki-67. The endpoints were to determine the prevalence of the PDEF protein expression and its correlation with these population?s prognostic factors. Results: The sample was constituted 46 patients and the median follow-up was 23,2 months. There was a trend towards loss of PDEF protein expression according to the patients? clinical stage. PDEF expression values were 30,3%, 25,8% and 14,7% in stages I, II and III, respectively. There was not a significant correlation with the lost of PDEF protein expression and the clinical and pathological characteristics along with the proportion of Ki-67 proliferative marker and the patient?s global survival. Conclusions: These results suggest that the PDEF protein, member of the Ets family, act as a repression gene of cancer colon-rectal carcinogenesis. The PDEF expression analysis can be an interesting prognostic marker and a therapeutic target. New studies with more patients and a long follow-up are necessary to validate these results.
Base te?rica: O c?ncer colorretal ? a terceira neoplasia mais freq?ente no mundo ocidental com taxas de mortalidade ainda consideras altas. A progress?o do tecido epitelial do c?lon normal at? o est?gio de neoplasia invasiva ? acompanhada de uma s?rie de processos celulares chamados carcinog?nese que, ao fim, convergem para o potencial de migra??o celular e metastatiza??o. As vias metab?licas da fam?lia dos genes Ets (E26 - sequ?ncia espec?fica de transcri??o), identificada em uma grande diversidade de esp?cies, contribuem neste processo, ativando ou reprimindo transcri??es de DNA. Em especial o fator derivado prost?tico (PDEF) cujo progn?stico e mecanismo de a??o relacionado ao c?ncer colorretal, at? o momento, n?o est? claramente elucidado. M?todos: Uma coorte retrospectiva de pacientes com carcinoma colorretal est?gios de I a III, diagnosticados e tratados na mesma institui??o de 2002 a 2008, foi estudada. As caracter?sticas histol?gicas e cl?nicas, bem como os dados de evolu??o e a sobrevida foram revisados. A an?lise de imunoistoqu?mica com m?todo de matriz de amostras teciduais (TMA) e a quantifica??o por captura de imagem foram realizadas em blocos representativos do tumor com anticorpos para detec??o da express?o da prote?na PDEF e Ki-67. Os objetivos eram detectar a preval?ncia da express?o da prote?na PDEF e sua correla??o com fatores progn?sticos nesta popula??o. Resultados: A amostra foi constitu?da de 46 pacientes e tempo de seguimento mediano de 23,2 meses. Houve tend?ncia a perda da prote?na PDEF conforme o estadiamento dos pacientes sendo a express?o de 30,3%, 25,8% e 14,7% nos est?gio I, II e III, respectivamente, P=0,416. N?o encontrou-se associa??o significativa entre os achados cl?nico-patol?gicos, a dosagem do marcador de prolifera??o celular Ki-67 e a sobrevida global dos pacientes com a perda da express?o da prote?na PDEF. Conclus?es: Os resultados sugerem que a prote?na PDEF, membro da fam?lia Ets, atue como gene repressor da carcinog?nese do c?ncer colorretal. A an?lise de sua express?o pode se tornar um interessante marcador progn?stico e alvo terap?utico. S?o necess?rios novos estudos com amostras maiores e tempo de seguimento mais longo a fim de validar estes achados.
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Anschau, Fernando. "Do carcinoma cervical in situ ao invasor : o papel da express?o da P16INK4a na progress?o e na recorr?ncia." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2008. http://tede2.pucrs.br/tede2/handle/tede/1494.
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Previous issue date: 2008-08-18A fim de investigar a express?o da p16INK4a no carcinoma cervical e sua rela??o com a transforma??o de carcinoma in situ em invasor, al?m de seu papel na recorr?ncia das les?es cervicais, uma s?rie de 90 pacientes com carcinoma cervical (49 com les?o in situ e 41 com les?o invasora) foi selecionada, entre julho de 2001 e setembro de 2002. Os grupos com les?o in situ e invasora foram pareados para uma s?rie de vari?veis de risco do c?ncer cervical e as pacientes mantidas em acompanhamento por 60 meses. As visitas de acompanhamento ocorreram a cada 6 meses nos primeiros tr?s anos e anualmente at? o quinto ano. 87,9% das pacientes com les?o invasora apresentavam super express?o da p16INK4a, em compara??o com 37,6% daquelas com les?o in situ (X2:13,68; 2GL; p=0,0002; OR:12,08), demonstrando ser a super express?o da p16INK4a um risco de invas?o da membrana basal por c?lulas displ?sicas. Tamb?m observamos associa??o entre super express?o da p16INK4a e estadiamento do c?ncer (X2:18,38; 6GL; p=0,0003). A an?lise prospectiva, quando controlada a intera??o com os grupos de les?o cervical (c?lculo de regress?o de Cox), demonstra risco de 4,83 atribu?do ? super express?o da p16INK4a para recorr?ncia, mas sem signific?ncia estat?stica (p=0,14).
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Benkhaled, Imad. "Mise au point d’une chaîne de capture/ restitution stéréoscopique d’images couleurs : application à la conception d’interfaces adaptées aux déficients visuels." Thesis, Montpellier, 2018. http://www.theses.fr/2018MONTS037/document.
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Les travaux de cette thèse sont menés dans le cadre d’un projet porté par le centre de recherche LGI2P, (IMT Mines Ales). L’objectif final de ce projet vise à permettre le retour à l’emploi et à améliorer le quotidien des personnes malvoyantes atteintes de rétinite pigmentaire et de glaucome. Le dispositif final est conçu pour aider les personnes dans leurs activités en mobilité : détection d’obstacles, recherche d’indices visuels, en adressant les problèmes liés à l’éblouissement et à l’héméralopie dont souffrent ces utilisateurs potentiels.La contribution de cette thèse au projet se situe sur plusieurs plans. Tout d’abord, il était demandé de définir les paramètres caractérisant la vision résiduelle de chaque utilisateur. En effet, chaque patient a ses propres conditions de confort lumineux, qui dépendent en particulier de l’état d’avancement de sa pathologie : à chaque étape de l’évolution de leur maladie, les patients ont des limites spécifiques de luminosité minimale en dessous de laquelle ils ne perçoivent plus les détails dans une scène, et aussi de la luminosité maximale au-dessus de laquelle ils ressentent gêne et douleur. La définition de ces limitations en luminosité va permettre de paramétrer le dispositif et l’adapter à chaque utilisateur. Mais il n’existe pas de méthode pour mesurer ces niveaux de luminance limite. Nous avons donc participé à la conception et au développement de tests dédiés, et à la réalisation d’essais sur des sujets déficients visuels, dans le cadre d’un essai clinique piloté par le CHU de Nîmes et l’ARAMAV (institut spécialisé dans la rééducation fonctionnelle basse vision), pour l’aspect médical. Nous avons également proposé un nouveau test pour mesurer la sensibilité au contraste chromatique, toujours dans le but de mieux adapter les images affichées à la vision des utilisateurs.Nous avons ensuite travaillé sur la mise au point d’un prototype du dispositif (caméras et visiocasque de réalité virtuelle). Pour cela, nous avons dû choisir les équipements de capture et d’affichage d’images. Un travail de calibration colorimétrique sur ces équipements nous permet de relier grandeurs numériques (code RGB) et grandeurs physiques (luminance et chrominance). Cette étape est nécessaire pour réaliser les tests précités dans des conditions physiquement connues. Elle nous permet également de définir les caractéristiques physiques que devront posséder les équipements qui seront choisis pour réaliser le produit final, s’ils sont différents de ceux utilisés pendant nos travaux.Enfin, nous avons abordé la question des traitements à appliquer au signal capturé par la caméra. Nous avons proposé des traitements en temps réel sur la luminosité dans le but d’augmenter la luminosité dans les zones sombres de l’image et de baisser la luminosité dans les zones qui éblouissent le patient. Nous avons montré les limitations de l’imagerie classique et la nécessité de travailler sur des images HDR (high dynamic range) Nous avons comparé plusieurs méthodes pour permettre l’affichage de ces images HDR sur les écrans de plus faible dynamique, en recherchant les caractéristiques de l’image que ces méthodes doivent préserver au mieux, et en prenant en compte les performances visuelles des utilisateurs potentiels. Nous avons aussi proposé des traitements sur la couleur en augmentant le contraste et la saturation pour rendre les images mieux perceptibles par les patients qui souffrent de troubles de vision des couleursThis thesis is part of a project conducted by the LGI2P research center (IMT Mines Ales). The project's final aim is to help people with vision disorders suffering from retinitis pigmentosa and glaucoma get back to work and improve their daily lives. The final device is designed to help people in their mobility activities: detecting obstacles, searching for visual signals, by addressing problems related to dazzling and haemeralopia affecting these potential users.The research of this thesis has several contributions to the project. First of all, parameters characterizing the residual vision of each user had to be defined. Indeed, each patient has his own light comfort conditions, which depend in particular on his pathology's progress: at each stage of the evolution of their disease, patients have specific minimum luminosity limits below which they no longer perceive the details in a scene, and also on the maximum luminosity above which they feel discomfort and pain. The definition of these limitations in luminosity will make it possible to parameterize the device and adapt it to each user. But there is no method to measure these limiting luminance levels. We have therefore participated in the design and development of specialized tests, and in the conduct of trials on visually impaired subjects, as part of a clinical trial led by the Nîmes University Hospital and the ARAMAV (institute specializing in low vision functional rehabilitation), for medical research. We have also proposed a new test to measure sensitivity to chromatic contrast, always with the aim of better adjusting the images displayed to users' vision.Then, we developed a prototype of the device (cameras and virtual reality video headset). In order to achieve these results, we had to choose the image capture and display equipment. A colorimetric calibration work on these equipments allowed us to link digital quantities (RGB code) and physical quantities (luminance and chrominance). This stage is required to perform the above tests under physically known conditions. It also allowed us to define the physical characteristics of the equipment that would be selected to produce the final product, whether they are different from those required during our work.Finally, we discussed the processing to be applied to the signal captured by the camera. We have proposed real-time brightness treatments to increase brightness in dark areas of the image and decrease brightness in areas that dazzle the patient. We have presented the limitations of conventional imaging and the necessity to work on HDR (high dynamic range) images. We have compared several methods to allow the display of these HDR images on screens with lower dynamic range, looking for the image characteristics that these methods should better preserve, and taking into consideration the visual performance of potential users. We have also suggested color treatments by increasing contrast and saturation to make images more perceptible to patients with color vision disorders
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Jalal, Ghita. "Réification des propriétés visuelles pour les tâches de composition." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLS554/document.
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Les graphistes utilisent des propriétés visuelles comme la couleur, la police de caractères typographiques, la taille ou la position pour construire et communiquer leurs concepts visuels. La plupart des outils numériques de composition considèrent les propriétés visuelles comme simples attributs. Ces propriétés sont accessibles, quand l’objet est sélectionné,via des boîtes de dialogue, sans êtres considérées comme objets indépendants dans l’interface. Cette thèse porte sur la réification des propriétés visuelles des objets graphiques pour les tâches de composition.Je soutiens l’importance de créer des outils de composition qui permettent de manipuler les propriétés visuelles directement, sans passer par des menus ou des boîtes de dialogue.La manipulation des propriétés visuelles dépend de la perception des propriétés, de l’expertise de l’utilisateur et de leur représentations sur l’interface.Elle dépend également des outils existants pour manipuler les propriétés visuelles dans le contexte de travail. Les outils numériques de composition utilisent principalement des boîtes de dialogue. Certains travaux de recherche soutiennent l’importance de faire évoluer les boîtes de dialogues, et proposent des boîtes de dialogues plus interactives. D’autres travaux proposent de nouvelles manières de représenter et de manipuler les propriétés visuelles.Les graphistes préfèrent les outils qui agissent directement sur les propriétés visuelles. Ils adaptent ces propriétés, pour les utiliser comme outils leur permettant de compléter des tâches comme l’alignement et l’espacement.Les artistes et les designers travaillent en développant leurs propres représentations des espaces de couleur. Ils manipulent chaque couleur, dans le contexte des autres couleurs utilisées dans le même document.Leurs choix de couleur dépendent également d’autres propriétés visuelles comme la texture, la matière, ou la luminosité. En créant leurs mise en page, les graphistes établissent des liens entre les propriétés spatiales comme la position, la taille, et la superposition des éléments graphiques. Ils créent des règles pour définir comment ces liens évoluent dans un même document, ou entre différents documents.Deux groupes d’outils proposent des exemples de la réification des propriétés visuelles pour des tâches de composition. Deux études avec des designers présentent différentes utilisations envisagées pour ces outils.Chaque utilisateur présente des exemples de projets spécifiques dans lesquels ces outils faciliteront leurs tâches de composition, et comment ils leur permettront d’obtenir les rendus recherchésIn this dissertation, I argue that visual composition tools should reify visual properties— that is, create them into first-class, interactive objects that designers can manipulate, directly in the document. Artists and designers use visual properties such as color, typography,size, and position to create novel composition concepts. Most visual composition tools treat graphical elements as objects— but not their visual properties. The latter are simply attributes of graphical elements, usually accessible on demand, through property sheets or dialog boxes, rather than as independen tinteractive objects.I begin with an introduction, where I outline the dissertation and summarise the main contributions.Then, I present a conceptual overview, where I argue that perception, expertise, theories and existing representations of visual properties affect how designers manipulate them in their work. In the related worksection, I identify the main approaches visual composition tools propose to manipulate visual properties.I explain the advantages and limitations of these approaches.I present a comparative structured observation study (Chapter 4) where graphic designers perform visual composition tasks in Adobe Illustrator. Designers preferred tools that provide direct access to visual properties.They use these visual properties to complete visual composition tasks such as alignment and distribution.I then present two interview studies (Chapter5, Chapter 6) that examined artists’ and designers’practices as they manipulate color and create layouts in their projects. Artists and designers create personal color representations. They manipulate each color in the context of its surrounding graphical elements,and combine it with other visual properties such as texture. As they create their layouts, designers establish links among visual properties such as size,position, and layering of graphical elements. They define rules for how these properties change in space,across instances of the same composition, or in time,across related compositions.Based on these observations, two groups of prototypes (Chapter 7) demonstrate how we can reify visual properties into first-class graphical objects. ColorPartner supports creating personal representations of the color manipulation process. Color Revealer permits creating informative color representations of how a process evolves. Palette Explorer supports manipulatingvisual properties of color swatches, such as size, position and layering, in the context of surrounding colors. Color Compositor supports manipulating the combination of colors and textures. Linkify supports linking visual properties directly in the documentand defining rules for how these links evolve as the content changes. Contextify supports creating rules that define how visual properties change across instances of a composition. In two follow up studies,designers explain how they intend to use these tools in their current projects..I conclude with a summary of the contributions(Chapter 8) and a discussions of the limitations and future work
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Flores, Marcelo Benedito da Silva 1969. "O aumento do fator de necrose tumoral-a induzido pela obesidade leva ao desenvolvimento do câncer de cólon em camundongos = Obesity-induced increase in tumor necrosis factor-a leads to development of colon cancer in mice." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/312401.
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Orientador: Jose Barreto Campello CarvalheiraTese (Doutorado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
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Resumo: O câncer colorretal é um dos maiores problemas de saúde em todo o mundo acometendo 1 milhão de pessoas por ano. O índice de morte associado a essa doença é de aproximadamente 33% no mundo desenvolvido. A associação entre a obesidade e o risco para o desenvolvimento desse câncer é observada tanto em homens quanto em mulheres. A inflamação e a hiperinsulinemia, condições verificadas na obesidade podem contribuir, a princípio, para o risco de desenvolvimento do câncer colorretal. O fator de necrose tumoral alfa (TNF-alfa) é um dos mais importantes mediadores da resposta inflamatória e sua alta expressão pelo tecido adiposo é verificada nas condições de obesidade tanto em modelos animais quanto em humanos. O TNF-alfa contribui para a desregulação da via da sinalização insulínica através da fosforilação em serina dos substratos do receptor desse hormônio (IRS) mediada pela ativação de quinases como c-jun N terminal quinase (JNK) e da quinase inibidora do fator nuclear NF-kB (IKK). A ativação dessas quinases induz a fosforilação inibitória do substrato 1 do receptor de insulina (IRS-1) através da serina 307 (Ser307). Esse mecanismo reduz a ativação da via da fosfatidilinositol 3-quinase (PI3K)/Akt e da proteína alvo da rapamicina em mamíferos (mTOR) mediada pela insulina. TNF-alfa foi, a princípio, identificado como um agente antitumoral. Atualmente essa citoquina é reconhecida como uma promotora da tumorigênese e que associa a inflamação ao câncer. A importância do TNF-alfa e de seus mediadores intracelulares, JNK e IKK, como promotores do câncer de cólon é corroborada por estudos farmacológicos que utilizaram o azoximetano (AOM) ou AOM associado ao dextran sulfato de sódio (DSS) como indutores do câncer colorretal. Ademais, o aumento das concentrações do TNF-alfa associado à obesidade é um mecanismo comprovado de aumento do câncer de fígado em modelos animais. Embora as evidências de que a inflamação e a hiperinsulinemia tenham um envolvimento em potencial na gênese tumoral mediada pela obesidade, à avaliação sistemática da contribuição independente desses fatores para o desenvolvimento do câncer colorretal ainda é inconsistente. Neste trabalho, foi avaliado se a obesidade modulou a sinalização insulínica e a inflamação em tecido colônico e nos tumores colorretais. Foi mostrado que a resposta inflamatória anormal induzida pela obesidade promoveu de forma contundente o câncer colorretal
Abstract: Colorectal cancer (CRC) remains a major health burden with more than 1 million cases worldwide and a disease-specific mortality of approximately 33% in the developed world. The association between obesity and the risk for CRC development is observed in both men and women. In addition to its association with obesity, inflammation and hyperinsulinemia also primarily may contribute to the risk for development of CRC. Among the major mediators of the inflammatory response is tumor necrosis factor (TNF-alfa), whose overexpression in adipose tissue is a common feature in human and animal models of obesity. TNF-alfa contributes to the deregulation of the insulin-signaling pathway, including serine phosphorylation of insulin-receptor substrate (IRS) proteins by kinases such as c-jun N terminal kinase (JNK) and inhibitor of nuclear factor-kB kinase (IKK). JNK and IKK activation induce inhibitory serine 307(Ser307) phosphorylation of IRS-1, which decreases insulin-mediated phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway activation. TNF-alfa, first identified as an antitumor agent, now also is recognized as a tumor-promoting cytokine that links inflammation and cancer. The importance of TNF-alfa and its intracellular mediators, such as JNK and IKK, as colonic tumorigenic promoters is strengthened by knockout and pharmacologic studies, using azoxymethane (AOM) or AOM combined with dextran sulfate sodium (DSS) as inducers of colorectal carcinogenesis. Furthermore, the increased TNF-alfa levels associated with obesity are a potent liver tumor promoter in mice. Although the evidence for the potential involvement of inflammation and hyperinsulinemia in the development of obesity-mediated cancer is quite extensive, a systematic evaluation of the independent contribution of these factors to CRC development is lacking. Here, we examined whether obesity modulates insulin signaling and inflammation in the colon and CRC. We show that the obesity induced abnormal inflammatory response strongly promotes CRC
Doutorado
Clinica Medica
Doutor em Clínica Médica
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Ekman, Frida. "Hydrodynamic modelling of fate and transport of natural organic matter and per- and polyfluoroalkyl substances in Lake Ekoln." Thesis, Uppsala universitet, Institutionen för geovetenskaper, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-433803.
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Societies are facing great challenges with obtaining a good quality and quantity of drinking water in the context of climate change. Increases in natural organic matter (NOM) and per- and polyfluoroalkyl substances (PFAS) have been observed in lakes and drinking water the past years, which is of great concern for water treatment plants in Sweden. It is therefore vital to increase the knowledge regarding the distribution of these substances in the environment. The main objective of this project was therefore to further develop a hydrodynamic model for lake Ekoln by including transportation and degradation of NOM. This was to be done by calibrating the model in terms of total organic carbon (TOC) and Water colour (Colour). A second objective was to investigate the requirements to successfully model PFAS in Ekoln for future studies. The study was done using the model MIKE 3 FM, developed by the Danish Hydraulic Institute (DHI) The two variables TOC and Colour, were calibrated separately for the period of February 2017 to September 2018. For TOC the within-lake processes were decay and sedimentation. These were described using a reference decay constant for 20 °C (k0), that was scaled using the Arrhenius temperature coefficient (θ), and sedimentation was represented by a settling velocity (vsm). For Colour the included process was photooxidation. This process was described using a maximum photooxidation rate (kphoto) that was scaled using the Monod relation including parameters for minimum photosynthetically active radiation (PAR) necessary for photooxidation to occur (Imin) and a PAR half saturation constant (I1/2). The calibration of TOC resulted in the following best fit parameters for k0 of 0.001 d-1, θ of 1.07 and vsm of 0.001 md-1. The calibration of Colour resulted in the following best fit parameters for kphoto of 0.0125 d-1, Imin of 0 µmol photons m-2s-1 and I1/2 of 4 µmol photons m-2s-1. Overall it can be concluded that the chosen processes managed to capture the seasonal variations of TOC and Colour, and the calibrated parameter values are in line with similar studies. The assumption of not including autochthonous input proved to be the biggest source of error in the calibration of TOC but proved to have a minor influence on the calibration of Colour. To achieve a more realistic representation of photooxidation in the vertical profile, for the simulation of Colour, more processes should be considered to be added in the model in future studies. The results presented in this study contributes with increased knowledge of carbon budgets in lakes and can be used to predict water quality hazards connected to climate change and extreme events. The limited access to PFAS data for Ekoln, constrained the study of PFAS and only two sources could therefore be studied: The sewage treatment plant Kungsängsverket and precipitation. The results showed that the simulated concentrations of PFAS in Ekoln only accounted for 40 % of the observed concentrations. It could further be concluded that the contribution from precipitation is negligible. For future studies it is judged to be vital to include Fyrisån as a PFAS source, and to look into processes that influence PFAS distribution, such as sedimentation and adsorption to organic matter.Samhällen står idag inför stora utmaningar vad gäller att tillhandahålla god kvalitet och kvantitet av dricksvatten under rådande klimatförändringar. De senaste åren har det observerats ökande halter av naturligt organiskt material (NOM) och per- och polyfluorerade alkylsubstanser (PFAS) i sjöar och dricksvatten, vilket är bekymmersamt för Sveriges vattenreningsverk. Det är därför av största vikt att öka kunskapen om dessa ämnens distribution i miljön. Huvudsyftet med denna studie var därför att vidareutveckla en hydrodynamisk modell för sjön Ekoln så att den även inkluderar transporten och nedbrytningen av NOM. Detta utfördes genom att kalibrera modellen för totalt organisk kol (TOC) och Vattenfärg (Färg). Ett andra syfte var att undersöka vilka förutsättningar som krävs för att kunna modellera PFAS på ett korrekt sätt i Ekoln. Studien utfördes i modellverktyget MIKE 3 FM, utvecklat av DHI. De två variablerna TOC och Färg kalibrerades separat för perioden februari 2017 – september 2018. Processerna som valdes att påverka TOC var nedbrytning och sedimentation. Dessa processer beskrevs med hjälp av en referens-nedbrytningskonstant för 20 °C (k0), vilken anpassades med hjälp av Arrhenius temperaturkoefficient (θ) och sedimentation beskrevs med hjälp av en sedimentationshastighet (vsm). Färg påverkades endast av processen fotooxidation vilken beskrevs med en maximal hastighet för fotooxidation (kphoto) som anpassades med hjälp av Monods relation. Anpassningen skedde med hjälp av parametern för minimal fotosyntetiskt aktivt ljus (PAR) för att fotooxidation ska ske (Imin) samt en PAR halv mättnads konstant (I1/2) . Kalibreringen resulterade i värden för k0 av 0.001 d-1, θ av 1.07 och vsm av 0.001 md-1. Kalibreringen för Färg resulterade i värden för kphoto av 0.0125 d-1, Imin av 0 µmol fotoner m-2s-1 och I1/2 av 4 µmol fotoner m-2s-1. Det kan konstateras att de valda processerna lyckas med att beskriva säsongsvariationerna av både TOC och Färg och att de kalibrerade parametervärdena stämmer överens med litteraturen. Antagandet om att inte inkludera autoktont tillförsel av organiskt material (NOM från ytvatten), visade sig vara den största felkällan i simulering av TOC, men visade sig ha en mycket liten påverkan på simuleringen av Färg. För en mer realistisk bild av fotooxidations spridning i djupled, för simuleringen av Färg, bör fler processer övervägas att inkluderas i modellen för framtida studier. Studien av PFAS var begränsad av tillgången till data, vilket medförde att endast två källor av PFAS till Ekoln analyserades: reningsverket Kungsängsverket och nederbörd. Resultaten visade att den simulerade koncentrationen av PFAS endast motsvarade 40 % av den observerade. Vidare kunde det konstateras att tillförsel av PFAS från regn kan antas vara försumbar. För framtida studier av PFAS i Ekoln bedöms det vara avgörande att inkludera Fyrisån som en källa, samt att vidare undersöka processer som påverkar transporten av PFAS så som sedimentation och adsorption till organiskt material.
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Bautzova, Tereza. "Les systèmes microparticulaires pour la libération colonique." Phd thesis, Université de Franche-Comté, 2012. http://tel.archives-ouvertes.fr/tel-00830507.
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La maladie de Crohn et la rectocolite hémorragique font partie des maladies inflammatoires chroniques de l'intestin (MICI). Le principal objectif des traitements anti-inflammatoires est de favoriser la délivrance du principe actif localement, spécifiquement sur les zones enflammées et de limiter les effets indésirables. Ainsi, plusieurs systèmes à libération colonique de molécules actives ont été développés. Parmi eux, les pellets présentent de nombreux avantages par rapport aux formes solides unitaires conventionnelles. Dans un premier temps, des pellets comptant une substance anti-inflammatoire naturelle et nutritive, la rutine, ont été développés. L'intérêt de cette molécule est de réduire considérablement les effets secondaires qui constituent un véritable problème dans les traitements actuels des MICI. Les pellets ont été enrobé avec les polysaccharides naturels se dégradant avec la flore colonique. Les études in vitro ont démontré une libération minimale du principe actif au niveau de l'estomac et du petit intestin. Par contre, une libération rapide et totale a été observée lors de l'exposition des pellets dans les conditions du milieu colonique. Les résultats des tests in vivo ont démontré que la rutine a atténué considérablement l'inflammation au niveau de colon et les pellets enrobés ont été aussi efficaces que les pellets d'acide 5-aminosalicylique (5-ASA) commercialisés. L'administration orale de rutine via les pellets enrobés et préparés avec le chitosan semble être une approche prometteuse, permettant la libération du principe actif au niveau des zones enflammées, pour le traitement des MICI tout en réduisant les effets secondaires. Le deuxième but de notre travail était d'élucider l'impact du chitosan, un polymère mucoadhésif, sur l'efficacité thérapeutique. Les pellets de 5-ASA ont été préparés à partir de cellulose microcristalline avec ou sans chitosan. Un enrobage constitué d'un polymère pH dépendant,1' Eudragit® FS, a ensuite été réalisé autour du noyau. Les tests de dissolution ont montré que le principe actif n'était pas libéré du pellet après 2 h en milieu acide. En revanche,la libération était rapide dans un milieu simulant l'environnement colonique. Les tests ex vivo avec les pellets contenant le chitosan ont montré des propriétés mucoadhésives importantes qui ont été confirmées par la concentration élevée du métabolite de 5-ASA dans les tissus coloniques des rats. De plus, nous avons a démontré que les pellets permettaient d'atténuer de façon significative l'inflammation du côlon. Ainsi, les pellets bioadhésifs enrobés possèdent des propriétés bénéfiques supplémentaires pour la libération du 5-ASA au niveau du côlon par rapport à des formes multidoses commercialisées pour le traitement des MICI.
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Nobre, Camila Cristina Guimar?es. "Express?o do fator inibit?rio da migra??o de macr?fagos e do fator de permeabilidade vascular em les?es da c?rvice uterina induzidas pelo papilomav?rus humano." PROGRAMA DE P?S-GRADUA??O EM BIOLOGIA PARASIT?RIA, 2017. https://repositorio.ufrn.br/jspui/handle/123456789/24106.
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Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)
O c?ncer do colo do ?tero ? o terceiro tipo de c?ncer mais frequente em mulheres no Brasil, sendo a sua frequ?ncia menor apenas do que aquelas observadas para o c?ncer de pele n?o-melanoma e c?ncer de mama. O fator inibit?rio da migra??o de macr?fagos (MIF) ? produzido por diferentes tipos de c?lulas e participa de uma cadeia complexa de eventos que favorece o processo de carcinog?nese, sendo poss?vel observar um alto n?vel de express?o em quase todos os tipos de c?ncer humano, entre eles o c?ncer do colo do ?tero. O MIF tamb?m induz um aumento dose dependente na excre??o do fator de crescimento de endot?lio vascular (VEGF), que promove a angiog?nese, o crescimento tumoral e a migra??o dessas c?lulas no c?ncer do colo do ?tero. O objetivo deste estudo foi investigar a express?o de MIF e VEGF em pacientes que apresentam ou n?o les?es do colo do ?tero, no intuito de identificar a exist?ncia de uma rela??o direta entre a presen?a dos marcadores MIF e VEGF com o grau da les?o da paciente, bem como, com a presen?a do papilomav?rus humano (HPV). Foram inclu?das no estudo 45 mulheres que haviam sido encaminhadas para a Maternidade Escola Janu?rio Cicco com suspeita de les?es na c?rvice uterina. As pacientes que aceitaram participar do estudo responderam a um question?rio, para obten??o de dados s?cio-demograficos, seguido de exame cl?nico. Das mulheres que se submeteram a colposcopia, foram coletados dois fragmentos de tecido do colo do ?tero, um para an?lise histopatol?gica e outro para detec??o do HPV por PCR convencional. A express?o dos biomarcadores, MIF e VEGF, foi detectada pela t?cnica de imuno-histoqu?mica. A ?rea positiva de cada biomarcador foi lida e quantificada utilizando o programa ImageJ?, e o resultado foi analisado atrav?s dos programas de estat?stica SPSS? Statistics e GraphPad Prism. Das 45 pacientes inclu?das no estudo, 20% apresentaram resultado do exame dentro dos padr?es da normalidade, enquanto que 80% apresentaram algum tipo de altera??o no exame; sendo 35,55% les?es intraepiteliais de baixo grau (LIEBG) e 44,44% les?es intraepiteliais de alto grau (LIEAG). A taxa de preval?ncia global de infec??o genital pelo HPV foi de 80%, sendo 86,1% em pacientes com les?o. A express?o m?dia do VEGF e do MIF tiveram um aumento gradativo quando comparado entre as pacientes normais, LIEBG e LIEAG, correspondendo respectivamente aos seguintes valores, 19,62, 41,59 e 55,42 para o VEGF e 4,36, 9,44 e 22,86 para MIF. Foi poss?vel verificar uma correla??o positiva entre a express?o de MIF e VEGF (r = 0,523, p = <0,001). Por meio deste trabalho p?de-se concluir que o VEGF e o MIF est?o correlacionados e envolvidos no processo de displasia cervical, aumentando a sua express?o conforme ocorre a progress?o da les?o. No entanto, n?o foi poss?vel encontrar associa??o entre a presen?a do HPV e os n?veis de MIF e VEGF.
The cervical cancer is the third most frequent type of cancer in women in Brazil, and its frequency is only lower than those observed for non-melanoma skin cancer and breast cancer. Macrophage migration inhibitory factor (MIF) is produced by different cell types and participates in a complex chain of events that favors the process of carcinogenesis, being possible to observe a high expression level in almost all types of human cancer, such as in cervical cancer. MIF also induces a dose-dependent increase in the excretion of vascular endothelial growth factor (VEGF), which promotes angiogenesis, tumor growth and migration of these cells in cervical cancer. The objective of this study was to investigate the expression of MIF and VEGF in patients with or without cervical lesions, in order to identify the existence of a direct relationship between the presence of those markers with the degree of the lesion of the patient, as well, as with the presence of human papillomavirus (HPV). The study included 45 women who had been referred to the Maternity School Janu?rio Cicco with suspected lesions of the uterine cervix. Patients who accepted to participate in the study answered a questionnaire to obtain socio-demographic data, followed by clinical examination. Patients who agreed to participate in the study answered a questionnaire to obtain socio-demographic data, followed by clinical examination. Two cervix tissue fragments were collected from the women who underwent colposcopy, one for histopathological analysis and the other for HPV detection by conventional PCR. The expression of the biomarkers, MIF and VEGF, was detected by the immunohistochemical technique. The positive area of each biomarker was read and quantified using the ImageJ? program and the result was analyzed using the statistical program SPSS? Statistics and GraphPad Prism. Of the 45 patients included in the study, 20% showed no lesions, while 80% had some type of alteration in the exam; being 35.55% low-grade squamous intraepithelial lesion (LSIL) type and 44.44% highgrade squamous intraepithelial lesion (HSIL) type. The overall prevalence rate of genital HPV infection was 80%, with 86.1% in patients with lesions. Mean VEGF and MIF expression increased gradually when compared to normal patients, LSIL and HSIL, respectively, corresponding to the following values: 19.62, 41.59 and 55.42 for VEGF and 4.36, 9, 44 and 22.86 for MIF. A positive correlation between MIF and VEGF expression was found (r = 0.523, p = <0.001). Through this work it was concluded that VEGF and MIF are correlated and involved in the cervical dysplasia process, increasing its expression as the lesion progresses. However, it was not possible to find an association between the presence of HPV and the levels of MIF and VEGF.
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L'Huillier, Clement. "Etude de l'impact de supplémentations en acides aminés dans un modèle murin d'anorexie associé à une activité physique. Glutamine, but not branched-chain amino acids, restores intestinal barrier function during activity-based anorexia Influence of glutamine and branched-chain amino acids supplementation during refeeding in activity-based anorectic mice." Thesis, Normandie, 2019. http://www.theses.fr/2019NORMR123.
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L’anorexie mentale est un trouble majeur du comportement alimentaire décrit dans le DSM-V affectant en majorité les femmes et se caractérisant principalement par une réduction sévère de l’apport calorique. Elle est fréquemment associée à des troubles anxiodépressifs, une hyperactivité physique ainsi qu’à des troubles fonctionnels digestifs (TFI). Bien que sa prévalence soit faible, il s’agit de la pathologie mentale avec le plus fort taux de mortalité et un risque de rechute très élevé. Le but de cette thèse était de vérifier l’efficacité d’une supplémentation orale en acides aminés tels que la glutamine (Gln) et les acides aminés branchés (BCAA) dans un modèle murin activity-based anorexia (ABA). Ce dernier possède des caractéristiques très proches de ce qui est observé en clinique comme une perte de poids importante, une hyperactivité physique et une augmentation du temps de vidange gastrique. Chez les souris ABA, une supplémentation orale de sept jours en Gln a permis de restaurer, dans le colon, la perméabilité paracellulaire, la synthèse protéique totale et le taux en ARNm codant pour la mucine-2. En revanche, aucun effet bénéfique n’a été trouvé pour les BCAA qui semblaient même diminuer la synthèse protéique colique totale. Le poids et la composition corporelle n’ont pas été affectés par la Gln ou les BCAA, peut-être en raison d’un apport calorique trop faible. Pour vérifier cette hypothèse, après induction de l’anorexie par le modèle ABA, nous avons choisi de combiner une renutrition progressive avec une supplémentation en Gln ou en BCAA. La renutrition a restauré partiellement le poids et la masse maigre des souris ABA avec un rebond de masse grasse mais la Gln n’a montré aucun effet supplémentaire. En revanche, les BCAA tendaient à augmenter légèrement le regain de masse grasse, et donc le poids, par rapport à la renutrition. Les animaux ABA renourris ont retrouvé une leptinémie normale, sans effet des supplémentations, alors que seule la Gln a restauré le taux en triglycérides plasmatiques. Les BCAA, quant à eux, tendaient à augmenter la glycémie et la cholestérolémie. Les effets bénéfiques de la Gln observés dans le colon dans l’étude précédente ont été confirmés. La Gln a montré des effets supérieurs à la renutrition seule sur la restauration de la synthèse protéique totale, via la phosphorylation de la p70S6kinase, et l’expression de protéines des jonctions serrées telles que la claudine-1 et l’occludine. En revanche, les BCAA semblaient atténuer, voire supprimer, les effets bénéfiques de la renutrition seule. La Gln paraît donc intéressante pour l’optimisation des produits de renutrition destinés aux personnes anorexiques et l’effet inhibiteur des BCAA devra être étudié pour en comprendre les mécanismesAnorexia nervosa (AN) is one of the main eating disorders described in DSM-V occurring in female population and characterized by a severe caloric restriction. AN is quite frequently associated with mood disorders, physical hyperactivity and functional digestive disorders. In addition, AN has the highest mortality rate among all mental disorders and high risk of relapse. The aim of this thesis was to assess the effects of amino acids oral supplementations as glutamine (Gln) and branched-chain amino acids (BCAA) in the activity-based anorexia (ABA) mice model. ABA mice share some common features with AN patients as severe weight loss, physical hyperactivity and delayed gastric emptying. When supplied for seven days with Gln, paracellular permeability, total protein synthesis and mucin-2 mRNA level were restored in the ABA mice colon. However, no beneficial effect was found with BCAA which seemed decrease the colonic protein synthesis. Body weight and body composition were not affected by Gln or BCAA and we hypothesized that caloric supply was not enough to induce body weight gain. Thus, we aimed to test our hypothesis with a combination of a progressive refeeding and Gln or BCAA supplementation. Refeeding partially restored body weight and lean mass in ABA mice with a rebound of fat mass but Gln had no additional effect. Nevertheless, BCAA tended to increase fat mass, and then body weight, in comparison to refeeding alone. Plasma leptin was restored in refed mice, without an effect of amino acids supplementation, while only Gln normalized plasma triglycerides. BCAA supplementation tended to increase glycaemia and cholesterolemia. Here, we confirmed the beneficial effects of Gln in the colon observed in our first study. Gln amplified the effects of refeeding on colonic total protein synthesis, p70S6kinase phosphorylation and tight junction proteins as claudin-1 and occludin. Conversely, BCAA appeared to alleviate, or cancel, the benefits of refeeding on these parameters. Given these data, Gln is thought to be of interest in the optimization of refeeding products for anorectic patients and inhibitory effects of BCAA should be further studied
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Eskenazi, Sébastien. "On the stability of document analysis algorithms : application to hybrid document hashing technologies." Thesis, La Rochelle, 2016. http://www.theses.fr/2016LAROS019/document.
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Un nombre incalculable de documents est imprimé, numérisé, faxé, photographié chaque jour. Ces documents sont hybrides : ils existent sous forme papier et numérique. De plus les documents numériques peuvent être consultés et modifiés simultanément dans de nombreux endroits. Avec la disponibilité des logiciels d’édition d’image, il est devenu très facile de modifier ou de falsifier un document. Cela crée un besoin croissant pour un système d’authentification capable de traiter ces documents hybrides. Les solutions actuelles reposent sur des processus d’authentification séparés pour les documents papiers et numériques. D’autres solutions reposent sur une vérification visuelle et offrent seulement une sécurité partielle. Dans d’autres cas elles nécessitent que les documents sensibles soient stockés à l’extérieur des locaux de l’entreprise et un accès au réseau au moment de la vérification. Afin de surmonter tous ces problèmes, nous proposons de créer un algorithme de hachage sémantique pour les images de documents. Cet algorithme de hachage devrait fournir une signature compacte pour toutes les informations visuellement significatives contenues dans le document. Ce condensé permettra la création de systèmes de sécurité hybrides pour sécuriser tout le document. Ceci peut être réalisé grâce à des algorithmes d’analyse du document. Cependant ceux-ci ont besoin d’être porté à un niveau de performance sans précédent, en particulier leur fiabilité qui dépend de leur stabilité. Après avoir défini le contexte de l’étude et ce qu’est un algorithme stable, nous nous sommes attachés à produire des algorithmes stables pour la description de la mise en page, la segmentation d’un document, la reconnaissance de caractères et la description des zones graphiquesAn innumerable number of documents is being printed, scanned, faxed, photographed every day. These documents are hybrid : they exist as both hard copies and digital copies. Moreover their digital copies can be viewed and modified simultaneously in many places. With the availability of image modification software, it has become very easy to modify or forge a document. This creates a rising need for an authentication scheme capable of handling these hybrid documents. Current solutions rely on separate authentication schemes for paper and digital documents. Other solutions rely on manual visual verification and offer only partial security or require that sensitive documents be stored outside the company’s premises and a network access at the verification time. In order to overcome all these issues we propose to create a semantic hashing algorithm for document images. This hashing algorithm should provide a compact digest for all the visually significant information contained in the document. This digest will allow current hybrid security systems to secure all the document. This can be achieved thanks to document analysis algorithms. However those need to be brought to an unprecedented level of performance, in particular for their reliability which depends on their stability. After defining the context of this study and what is a stable algorithm, we focused on producing stable algorithms for layout description, document segmentation, character recognition and describing the graphical parts of a document
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Odun-Ayo, Frederick Oluwasheyi. "Inhibition of Colon Cancer in Mice by Microencapsulated Probiotic." Thesis, 2016. http://hdl.handle.net/10321/1757.
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Submitted in complete fulfillment for the Degree of Doctor of Philosophy in Biotechnology, Durban University of Technology, Durban, South Africa, 2016.Colon cancer is the third most common cancer worldwide with a high morbidity and mortality rate. Therapies are less effective during metastasis, therefore prevention and earlier detection is key to reducing the risk of colon cancer. Increased dietary fibre and probiotic intake is known to lower the risk of colon cancer. Probiotics are defined as “live microorganisms which when administered orally in an adequate amount confer a health benefit on the host”. The International Dairy Federation recommends a viable minimum level of 6–7 log10cfu/g in a probiotic product being consumed. Different biopolymer matrices have been used for encapsulation of probiotics; however, loss of viability is still a major challenge. Citrus pectin is a dietary fibre polysaccharide broken down into smaller fragments to form modified citrus pectin (MCP). The unique bioactivity of MCP against carcinogenesisis is linked to its sugar β-galactose inhibiting the cell signalling protein marker, galectin-3 (gal-3), which is intimately involved in endothelial cell morphogenesis. The vascular endothelial growth factor (VEGF) signalling, which invariably drives angiogenesis can be activated when gal-3 binds to integrins. The bioactivity and uptake of MCP may be improved through a novel approach if conjoined with a supplement for example probiotic. Therefore, the synergistic inhibitory effect of modified citrus pectin alginate (MCPA) probiotic microbeads on gal-3 and VEGF in an azoxymethane (AOM) induced colon carcinogenesis Balb/c mouse model was investigated. A microencapsulation process was used to produce a MCPA microbead containing probiotic, Lactobacillus acidophilus ATCC 4356. Efficiency of the microbead was evaluated in vitro (simulated conditions) and in vivo (Balb/c mouse model). Genomic identification of faecal lactobacilli samples from the treated mice was analyzed. Optimization of AOM dose-time with 10 and 15 mg/kg AOM intraperitoneal (ip) administered to Balb/c mice for 2 and 4 weeks were performed. The optimal AOM dose was initiated prior to intake of MCPA, AP (alginate calcium) probiotic microbeads and MCP in Balb/c mice for 16 weeks; samples were analyzed for colon histopathology and immunohistochemistry. The MCPA probiotic microbeads significantly enhanced the viability of L. acidophilus ATCC 4356 compared to the AP microbeads in vitro (p< 0.05). Exposure of the MCPA probiotic microbeads to 3 h of simulated gastric juice (SGJ) resulted in 82.7% survival of L. acidophilus ATCC 4356. Also, the faecal lactobacilli count in the MCPA probiotic treated mice significantly increased after 28 days by 10.2% compared to the AP probiotic, MCP treated and control mice (p< 0.0001). A total of 4DNA encoding 16S rRNA gene closest to the genera namely Lactobacillus, Bacillus, Enterococcus and Bifidobacterium were identified from faecal samples of the colon cancer-induced Balb/c mice. Azoxymethane at 15 mg/kg for 4 weeks induced optimal gal-3 and VEGF immunoexpression. Furthermore, MCPA probiotic treatment significantly reduced gal-3 immunoexpression in the colon of AOM induced cancer Balb/c mice compared to the control mice (p< 0.0001). The immunoexpresion of VEGF in the MCPA and AP probiotic treated groups was weakly positive and significantly reduced when compared to the control group (p<0.05), while the MCP treated group was barely positive (p< 0.001). Modified citrus pectin alginate is a novel effective means of oral delivery of bacterial cells and bioactive compounds. It has a good biodegradability, inexpensive, non-toxic, proven efficiency, and stability at low temperatures warranting its use as a drug carrier by pharmaceuticals. Modified citrus pectin alginate probiotic microbeads increase bioactivity and chemoprevention against colon pre-cancerous lesions and adenocarcinoma through inhibition of gal-3 and VEGF in the mouse model. Modified citrus pectin alginate can be used in probiotic therapy, which may improve the prevention of colon cancer.
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KUO, HSIN-YING, and 郭欣穎. "Preventive potential of carnosine on colon carcinogenesis in mice." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/q2dqp8.
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YANG, RAN. "Effect of frying oil consumption on colon tumorigenesis in mice." 2019. https://scholarworks.umass.edu/masters_theses_2/809.
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Deep-frying is now a popular cooking method all over the world due to its low cost and time-saving property. It also provides special and likeable flavor, helps prolong shelf lives of commercial products, and offering food products of stable quality. However, by- products formed during the frying process, such as malonaldehyde, were shown to be harmful to human health. Such compounds can be taken in when having fried foods, potentially inducing or promoting some diseases. However, there is limited research studying the direct effects of frying oil consumption on cancer. In order to have a better understanding of the effects on cancer by frying oil, we used a well-established AOM/DSS- induced colon cancer animal model to study the impact by frying oil. After 10-week treatment with diet containing deep-frying oil (3.8% in diet) or with un-oxidized oil (10% in diet), the mice showed enhanced tumorigenesis in colon, where the total tumor burden significantly increased (4.5 ± 1.9 mm2 for the treatment group, compared with 0.5 ± 0.5 mm2 for the control group, P < 0.05). Also, the expression of pro-inflammatory and pro- tumorigenic cytokines (Mcp-1, Inf-γ, Il-6, Il-1β, Myc, Axin2 and Vegf) were increased in the mice treated with frying oil diets. Together the results showed that consumption of deep-frying oil promoted the colorectal cancer in mice, providing more detailed information for health instruction.
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Guo, Su Tang. "The role of inositol polyphosphate 4-phosphatase II (INPP4B) in the pathogeneis of colon ccancer." Thesis, 2018. http://hdl.handle.net/1959.13/1386333.
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Research Doctorate - Doctor of Philosophy (PhD)Colon cancer is one of the most common and deadly malignancies. Despite recent advances in early diagnosis and the development of novel treatment approaches, the overall survival of patients with metastatic colon cancers remains disappointing. Lots of investigations have discovered some pathways important during the initiation and progression of CRC. These include the WNT, RAS−MAPK, PI3K, TGF-β, P53 and DNA mismatch-repair pathways. But Aberrant activation of the phosphatidylinositol 3-kinase (PI3K) pathway is of particular importance in CRC development, progression and resistance to treatment, since many common genetic and epigenetic anomalies in the disease, such as amplification of epidermal growth factor receptor, activating mutations of KRAS and loss of phosphate and tensin homolog deleted on chromosome 10 (PTEN), converge on activation of PI3K signaling. Lipid and protein kinases promote PI3K signaling, whereas lipid and protein phosphatases suppress PI3K signaling (Manning and Toker, 2017; Newton and Trotman, 2014). Activation of PI3K signaling is negatively regulated by three classes of inositol polyphosphate phosphatases. (Rodgers et al., 2017). The inositol polyphosphate 3-phosphatase (3-phosphatase) PTEN dephosphorylates the 3-position of PI(3,4,5)P3 to generate PI(4,5)P2 (Gericke et al., 2013; Kurokawa et al., 2012), whereas 5-phosphatases, such as Src homology 2-containing inositol 5- phosphatase (SHIP) and phosphatidylinositol 4,5-bisphosphate 5-phosphatase (PIB5PA)/proline-rich inositol polyphosphate phosphatase (PIPP) dephosphorylate the 5-position to produce PI(3,4)P2 (Park et al., 2001). The latter is in turn subjected to dephosphorylation by inositol polyphosphate 4-phosphatase type I (INPP4A) and type II (INPP4B) at the 4-position to generate PI(3)P, thus terminating PI3K signaling. During the past decades, INPP4B has been well established to be a tumor suppressor in a number of cancers. However, we have found that it is frequently upregulated in human colon cancer cells. Silencing of INPP4B blocks activation of Akt and serum- and glucocorticoid-regulated kinase 3 (SGK3), inhibits colon cancer cell proliferation and retards colon cancer xenograft growth. Conversely, overexpression of INPP4B increases proliferation and triggers anchorage-independent growth of normal colon epithelial cells. Moreover, we demonstrate that the effect of INPP4B on Akt and SGK3 is associated with inactivation of phosphate and tensin homolog through its protein phosphatase activity and that the increase in INPP4B is due to Ets-1-mediated transcriptional upregulation in colon cancer cells. To further consolidate the result, two genetically modified mouse model were generated by CRISP/CAS9 technique. The INPP4B+/-; VillinCre+/- mouse strain in which INPP4B expression limited to colon epithelium display increased proliferation potential than wild-type mice. Moreover, these mice appeared to be more sensitive to Colitis induced by Dextran Sulfate Sodium (DSS). Nevertheless, when these mice were crossed with adenomatous polyposis coli (APC) mutant mice, the resulting INPP4B+/-; VillinCre+/-; APC+/- mice exhibited increased tumorigenesis in colon. Although results with these transgenic mouse strains are still under way, the available results strongly support the oncogenic role of INPP4B in colon cancer. In this study, we also discovered and cloned a novel small transcript variant of INPP4B generated by alternative splicing in cells of human colon and breast epithelium origins, which we have named INPP4B-S( INPP4B-short). Moreover, we found that INPP4B-S is translated into a protein product that is located to the cytoplasm and promotes proliferation in colon and breast cancer cells. To the best of our knowledge, this is the first time that INPP4B-S has been cloned and described in detail. It seems that INPP4B-S has an additive effect to INPP4B-FL (INPP4B-full length).
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Chen, Yu-Hong, and 陳昱宏. "Gallic acid inhibits the metastasis of colon cancer cells in mice." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/83634712995523223178.
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碩士中山醫學大學
生化暨生物科技研究所
103
The colon cancer is cancer mortality rate death third, compare with other cancers as lung cancer, the large intestine and the colon cancer sieves examines is may prevent. If early discover colon cancer , the cure rate may reach as high as 90%.therefore how to cure the colon cancer and the discussion colon cancer cancer-causing possible, is clinical important research direction. The treatment of colon cancer operation excision as principle, chemistry treatment for assist, but cancer later period the survival rate low and after operation the cell easy to transfer, Merge traditional chemistry medicine treatment has serious side effect etc., make patient''s cure rate is low and relapse high. Therefore, the development a new medicine assists the treatment, the postponement or the suppression cancer development is essential. The natural anti-cancer research is the recent tidal current, gallic acid (GA) is the natural anti-oxidation ability phenolic compounds, can find in galla chinensis, lacquer tree, hamamelis , tea, ficus elastica discover...etc, be confirmed the toxicity responds poisonous to the cancer cell, but will not hurt the healthy cell. We conducted MTT assay, using the DLD-1 cancer of the intestines cell line to treated with different doses of GA, 3-OMGA, the 4-OMGA, observations DLD-1 cell viability, discovered three medicines do not have the obvious cell toxicity, then carries on the DLD-1 cell wound healing assay, we found that the long time, GA cells crawling ability weaker; Then in the metastasis experiment, observe from the transwell assay result, GA suppression cell transfer ability also is the effect best; In order to clarify its numerator mechanism, we analysis cytoskeletal proteins, adhesion molecules, and the specificity of inhibition of Src signal pathway to confirmed, GA via regulating CD44 signaling pathway effectively inhibited DLD-1 colon cancer cell metastasis effect. Finally also confirmed at the animal experimentation that, GA can inhibited cancer cell DLD-1 metastasis ability. According to this result GA has anti-colon cancer of the metastasis ability, future might apply in chemistry prevention becomes develops the new medicine.
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Wang, Su-Yao, and 王素瑤. "Wheat bran extracts induced apoptosis in human colon cancer cells and suppressed AOM-induced colon carcinogenesis in ICR mice." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/45253616407918266546.
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碩士國立高雄海洋科技大學
水產食品科學研究所
102
C13, C15 and C17 are mixture extracts from wheat bran (WB) that they have been shown to have anti-oxidation and anti-cancer activities. However, the molecular mechanisms haven’t been clear yet. In this study, we evaluated the anti-carcinogenic effects and related molecular mechanisms on dietary those compound in azoxymethane (AOM) induced colon carcinogenesis. And fed normal diet on control group and there different concentrations of C13, C15 and C17 were containing on diet for experimented group. All mice were sacrificed at the 6th week and we found dietary administrated C15 with significantly inhibit AOM-induced ACF (aberrant crypt foci) formation. Furthermore, oral administration of C15 inhibited iNOS gene expression. Moreover, we also investigated the molecular mechanisms of C15 induced apoptosis in C15 treated HCT-116 cells. The results showed that treatments were with 10 μg/mL of C15 in the HCT-116 cells significantly inhibit the HCT-116 cell growth and induce apoptosis. By Western blotting analysis,C15 caused the activation caspase-3 and caspase-9, and cleavage of PARP.Taken together, these findings suggested that C15 caused HCT-116 cells apoptosis via a modution of mitochondria were involving ROS production,caspase activation. On the basis of anti-cancer activity of C15, it may be feasible to develop chemo-preventive and agent from edible WB chemotherapeutic.
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Lin, Jingwen. "Effect of Jackfruit-Derived Extract Consumption on Colitis-Associated Colon Tumorigenesis in Mice." 2020. https://scholarworks.umass.edu/masters_theses_2/974.
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Colorectal cancer is the third most common cancer and the fourth most common cause of cancer-related death in the world. The global burden of colorectal cancer is also expected to increase by 60%, to over 2.2 million new cases and 1.1 million annual deaths, by the year 2030. Jackfruit is known for its packed nutrition including many antioxidants: vitamin C, carotenoids and flavanones. It has also been used in traditional medicine due to its potential protection against many chronic diseases. However, there is limited research studying the potential effect of jackfruit on colorectal cancer. Here, we used a well-established AOM/DSS mice model to investigate the impact of jackfruit-derived extracts on colitis-associated colorectal cancer. After 6-week treatment with diet containing 480 ppm jackfruit-derived extracts, the mice showed significantly alleviated colon tumorigenesis with a 46% decrease in tumor numbers of each mouse compared to vehicle group (2.1 ± 0.31 for 480 ppm jackfruit-derived fraction group vs 3.9 ± 0.67 for vehicle group, P < 0.05). The expression of the pro-inflammatory cytokines (Il-6 and Inf- γ) and pro-tumorigenic genes (Axin2, Vegf, Myc and Pcna) was also decreased in the group consuming 480 ppm jackfruit-derived extracts compared to the vehicle group. Together the results suggest that the consumption of jackfruit-derived extracts could protect against colitis-associated colorectal carcinogenesis in mice.
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Fan, Ruei-Fang, and 范睿芳. "Effects of Polysaccharides from Dendrobii Herb on Azoxymethane-induced colon cancer in BALB/c Mice." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/87487205722599872710.
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碩士中國醫藥大學
基礎醫學研究所碩士班
101
This study investigated the effect of Dendrobium Taiseed Tosnobile extracts (DTTPS) and Dendrobium tosaense extracts (DTPS) on preneoplastic lesions of colon and colon cancer in mice. Experiment 1, preneoplastic lesions of colon in mice were induced by intraperitoneal injection of azoxymethane (AOM), once in a week for two weeks. AOM injected mice were orally treated with DTTPS or DTPS for entire period, 6 weeks. DTTPS or DTPS caused a significant decrease in the number of aberrant crypt foci (ACF) and aberrant crypt (AC). RT-PCR analysis showed that the colonic mucosa of AOM-treated mice expressed high levels of mRNA of aldose reductase, glutathione reductase, heme oxygenase-1, iNOS and COX-2, and DTTPS or DTPS caused a marked decrease in the expression of these mRNA. Western blot analysis showed that the colonic mucosa of AOM-treated mice expressed high levels of protein of proliferating cell nuclear antigen, NF-E2 related factor-2, iNOS and COX-2, and DTTPS or DTPS caused a marked decrease in the expression of these proteins. These results indicated that DTTPS or DTPS might exert colon cancer preventive activities through antioxidation and anti-inflammation. Experiment 2, colon cancer in mice were induced by intraperitoneal injection of AOM, once in a week for six weeks. AOM injected mice were orally treated with DTTPS or DTPS for entire period, 24 weeks. DTTPS or DTPS caused a significant decrease in the number of ACF and AC. RT-PCR analysis showed that the colonic mucosa of AOM-treated mice expressed high levels of mRNA of insulin-like growth factor 1 receptor (IGF-IR), phosphase-glycogen synthase kinase 3 beta (pGSK3β ), pAkt-1 and ??-catenin, and DTTPS or DTPS caused a marked decrease in the expression of these mRNA. These results indicated that DTTPS or DTPS might exert inhibiting AOM-induced colon cancer. In conclusion, the present study demonstrated that both DTTPS and DTPS can act as an effective chemopreventive agents against colon cancer, and the potency of DTTPS was larger than DTPS.
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Chung, Minching, and 鐘敏菁. "Comparison of The Efficacy of Chemoprevention of Pterostilbene and 3′-hydroxypterostilbene in Inhibiting Proliferation of Colon Cancer Cells And Colon Carcinogenesis in Mice." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/70683189326735746177.
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碩士國立高雄海洋科技大學
水產食品科學研究所
101
Pterostilbene (3, 5-dimethoxy-4′-hydroxystilbene, PSB), a natural dimethylated analog of resveratrol, is known to have diverse pharmacological activities including anti-proliferation, anti-metastasis,and anti-inflammation activities. Previous studies demonstrated the effects of in vitro and in vivo studies of a potential anti-colon cancer agent of PSB. 3′-hydroxypterostilbene (3, 5-dimethoxy-3′, 4′-dihydroxystilbene, HPSB ) is the part metabolite of PSB in mice urine. HPSB is the presence of an additional hydroxyl group in position 3′ by hydroxylation. HPSB has been demonstrated to induce apoptosis. Here, we comparison with the ability of PSB and HPSB in inducing mechanisms of inhibiting proliferation of human colon cancer cells. MTT analysis demonstrated that PSB and HPSB was able to inhibit number of colon cancer cell growth and induce apoptosis. Biological activity of HPSB showed more potent in the growth inhibition in COLO205 cancer cells than PSB. Furthermore, HPSB show more potent antioxidant activity than PSB. We evaluate the ability of PSB and HPSB induced apoptosis in COLO205 cancer cells. Flow cytometry analysis show that PSB treatment increased sub-G1 cell population whereas HPSB with stronger apoptosis-inducing activity. PSB and HPSB induced apoptosis in COLO 205 cell through modulation of mitochondrial functions regulated by reactive oxygen species (ROS). PSB and HPSB induced caspase-3 activation, which is associated with the degration of DFF-45 (DNA fragmentation factor) and PARP (poly (ADP-riobse) polymerase). Indded, HPSB showed more strong inducing apoptosis than PSB. Flow cytometry analysis show that PSB treatment increased autophagy marker acidic vascular organelles (AVOs) formation whereas HPSB with stronger autophagy-inducing activity. Pretreatment Chloroquine (CQ an autophagy inhibitor) also significantly induce apoptosis in PSB or HPSB-treated cells. PSB and HPSB induced autophagy may play suppression role in apoptosis. The mechanisms of HPSB induced autophagy was through down-regulated PI3K-Akt and MAPKs signaling including decreased the phosphorylation of mTOR and p70S6K in COLO 205 cells. In a xenograft model, we evaluate the ability of PSB and HPSB inhibits the growth of colon cancer tumor xenografts in vivo. HPSB significantly decreased tumor growth, volume and weight of COLO 205 xenografts. In a AOM/DSS model, we evaluate the ability of PSB and HPSB biological activity inhibits the growth of colon cancer tumor and the underlying molecular mechanism in vivo. Our results shown that feeding HPSB (250 ppm and 500 ppm) for 10 weeks significantly reduced the tumor number and incidence in mouse colon. At the molecular levels, the results from western blot analysis showed that dietary HPSB exhibited the anti-inflammatory by decreasing the levels of iNOS and COX-2. Moreover, HPSB significantly decreased tumor growth through down-regulation of cyclin D1, MMP-9 and VEGF protein expression. In conclusion, we demonstrated that HPSB displayed more potent activity in inducing of apoptosis and autophagy than PSB. In addition, HPSB significantly decreased tumor growth, volume and weight through down-regulation of iNOS , COX-2, cyclin D1, MMP-9 and VEGF protein expression. HPSB displayed more potent activity on antioxidant activity than PSB.These results provide insight into might establish a molecular basis for the development of colon cancer therapeutic agents.
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You-Mei and 林友梅. "Modulatory effects of three konjac hydrolysate fractions on risk factors of colon cancer in Balb/c mice." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/06604109243760987460.
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碩士中山醫學大學
營養學研究所
95
Konjac, a soluble dietary fiber, is rich in glucomannan polysaccharides which is composed of D-glucose and D-mannose by ß-1,4 glycosidic bond. The purpose of this study was to compare the effect of Konjac（K）, Konjac hydrolysate fractions（F1, F2, F3; the degree of polymerization was 16, 8, 4 respectively）, inulin（I）, fructooligosaccharide（FO）, pectin（P）and cellulose（C）on the concentrations of short chain fatty acids（SCFA）in the feces, and the fecal enzyme, fecal water pH, the cytotoxicity and DNA damage on Caco-2 cell. The 6-wk-old Balb/c mice were randomly allocated to consume AIN-93 control fiber-free（FF）, and 5% K, F1, F2, F3, I, FO, P or C diet for three weeks. The feces of mice were collected for three days before sacrifice. The mice were sacrificed on the 22nd day. After sacrificing, the main organs was collected and weighted to analyze. There were no differences in body weight gain across groups. FO group has the highest daily food intake, and K group has the lowest food efficiency. FF group has the highest liver relative weight, and FO group has the highest cecum relative weight. All of the fiber used in this study can increase the daily feces dry weight. On the fecal enzyme, F1 group has the highest ß-glucosidase activation; P group has the highest ß-galactosidase activation; F2 group has the highest ß-glucuronidase activation. Compare the fecal SCFA, F3 group has the highest concentrations of fecal acetate, propionate, and butyrate. In the cytotoxicity on Caco-2 cell, all of the fiber used in this study can improve the fecal water induce cell toxicity. In the DNA damage on Caco-2 cell, P and I group has the lowest DNA damage. After using the H2O2 inducing DNA damage, F3 group has the best effect on prevent DNA from H2O2 damage. In conclusion, the Konjac hydrolysate fractions 3（DP≒4）could increase the concentrations of fecal short chain fatty acids, and prevent the Caco-2 cell from fecal water damage.
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Chein, Chia-Yi, and 簡佳怡. "Inhibitory Effect of proteins from Hypsizigus marmoreus on CT26 Colon Adenocarcinoma Cells and Metastasis in Balb/c Mice." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/89761337724577504075.
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碩士國立臺灣大學
食品科技研究所
93
Hypsizigus marmoreus (HM), one of popular dietary mushrooms, contains high protein, low calorie, and low fat and has long been consumed in Japan. Several researches indicate that HM has antitumor and antifungal activities. In the present study, HM was used to study its antitumor activity in vitro and in vivo. First, 40~80% ammonium sulfate precipitates of cold-salt water extracts from HM were prepared and incubated with mouse colon CT26 cells to observe the changes in growth inhibition (%) for 24, 48 and 72 hr by MTT assay, followed by the cell cycle arrest by a Flow Cytometry. Results showed that remarkable growth inhibition, higher than 68 %, was observed when CT26 cells were incubated with 600 μg/mL of 40~80 % ammonium sulfate precipitates of cold-salt water extracts from HM. In addition, cell cycle arrest of CT26 cells at G2/M phase and apoptosis were induced. In vivo, pulmonary metastasis of CT26 tumor on male Balb/c mice orally administrated for 13 days with doses (200, 400 and 600 mg/kg bw/day) of ammonium sulfate precipitates of cold-salt water extracts from HM and injected (iv) with 1×105 CT26 cells via a tail vein was established to observe the results. Metastasic lung nodules in experimental group fed with 200, 400 or 600 mg/kg bw/day decreased in a dose-dependent manner, revealing the significant (p<0.05) anti-metastatic effect of HM proteins. Histopathologic results in lung was consistent with the finding in vivo. Insignificant changes in renal and immunohistograph were observed in mice at those three dosages.
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Hsu, Pong-Yang, and 徐鵬洋. "The Effects of Rice Diets on Development of Colorectal Tumor in An Inflammation-Associated Mice Colon Carcinogenesis Model." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/25959150146217402710.
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