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Dissertations / Theses on the topic 'Mice as laboratory animals Fertility'

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Published: 10 December 2022
Last updated: 29 January 2023

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1

Agarwal, Rajat. "A model for minimizing cost for housing laboratory mice." [Gainesville, Fla.] : University of Florida, 2003. http://purl.fcla.edu/fcla/etd/UFE0001241.

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2

Hsu, Charlie Chun. "Isolation, characterization, and diagnosis of murine noroviruses, a newly recognized pathogen of mice." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4790.

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Thesis (Ph. D.)--University of Missouri-Columbia, 2007.
"December 2007" The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Vita. Includes bibliographical references.
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3

Filipovska-Naumovska, Emilija. "Development of methods for detection and eradication of mouse parvovirus from a laboratory mouse colony." Thesis, Filipovska-Naumovska, Emilija (2007) Development of methods for detection and eradication of mouse parvovirus from a laboratory mouse colony. PhD thesis, Murdoch University, 2007. https://researchrepository.murdoch.edu.au/id/eprint/676/.

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The mouse parvovirus designated MPV can infect laboratory mice and affect the humoral and cellular immune response of infected mice, reducing their value for biomedical and medical research. The development and maintenance of MPV-free mouse colonies for biomedical research is therefore essential and requires routine monitoring of the infection status of mice, using serological surveillance procedures. Recent experience in the Animal Resources Centre (ARC), a major supplier of mice to the medical research community in Australia, was that MPV infection was present but was not detectable with the serological tests that were then in routine use. This thesis reports the development of a polymerase chain reaction (PCR) assay for the detection of the MPV in the ARC mouse colonies, the genetic characteristics of the strain of MPV detected, the development of a recombinant virus protein that provided a suitable antigen for enzyme-linked immunosorbent assay (ELISA) and a Western immunoblot (WIB) assay for the detection of MPV antibodies, and use of these various assays to determine aspects of the epidemiology and pathogenicity of the infection that were critical to the eradication of virus infection and future immunological surveillance to ensure the absence of infection. The recombinant protein produced as an antigen was a biotinylated fusion protein, a truncated capsid protein of the strain of MPV detected in the ARC, and was produced using the PinpointTM vector and with expression in Escherichia coli. The protein was produced as an insoluble intracellular product within inclusion bodies and was solubilised using urea and purified. The purified protein was utilised as an antigen for ELISA and the WIB assays to detect virus antibody in infected mice. The outbreak of MPV infection in the ARC was used as an unique opportunity for assessment of the seroprevalence of MPV-1 infection in a large laboratory mouse colony and to utilise this data to determine the sampling size needed to reliably detect MPV-1 infection within such large laboratory mouse colonies. An overall seroprevalence of 16.5% was detected using the developed serological tests, but considerable variation in prevalence was detected in different mouse strains. The response to MPV infection of 4 different but common strains of mice was determined as a basis for developing appropriate surveillance procedures and the selection of appropriate sentinel animals. The effect of infection of these strains at different ages was also investigated. Virus replication was detected in tissues of all the mice strains infected (outbred ARC(s) and inbred C57BL/6JArc, BALB/c and BALB/c-Foxn1nu/Arc) as juveniles and adults, with the exception of C57BL/6JArc inoculated as adults. However, while seroconversion in mice inoculated as juveniles and adults was detected in ARC(s) and C57BL/6JArc mice, it was not detected in BALB/c mice. The high rate of seroconversion to MPV, the early and prolonged development of an immune response, and the lack of age differences in their susceptibility indicated that ARC(s) mice would provide reliable sentinels for the detection of MPV. The genomic nucleotide sequence of the ARC strain, excluding the terminal palindromic regions and the predicted amino acid sequences of the non-structural and structural proteins was determined. This strain was very similar (98-99% nucleotide identity) to the previously described MPV strains MPV-1a, MPV-1b and MPV -1c. The similarity suggested there were unlikely to be significant antigenic differences in the proteins of the ARC strain and those strains of MPV reported previously.
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4

Filipovska-Naumovska, Emilija. "Development of methods for detection and eradication of mouse parvovirus from a laboratory mouse colony." Filipovska-Naumovska, Emilija (2007) Development of methods for detection and eradication of mouse parvovirus from a laboratory mouse colony. PhD thesis, Murdoch University, 2007. http://researchrepository.murdoch.edu.au/676/.

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The mouse parvovirus designated MPV can infect laboratory mice and affect the humoral and cellular immune response of infected mice, reducing their value for biomedical and medical research. The development and maintenance of MPV-free mouse colonies for biomedical research is therefore essential and requires routine monitoring of the infection status of mice, using serological surveillance procedures. Recent experience in the Animal Resources Centre (ARC), a major supplier of mice to the medical research community in Australia, was that MPV infection was present but was not detectable with the serological tests that were then in routine use. This thesis reports the development of a polymerase chain reaction (PCR) assay for the detection of the MPV in the ARC mouse colonies, the genetic characteristics of the strain of MPV detected, the development of a recombinant virus protein that provided a suitable antigen for enzyme-linked immunosorbent assay (ELISA) and a Western immunoblot (WIB) assay for the detection of MPV antibodies, and use of these various assays to determine aspects of the epidemiology and pathogenicity of the infection that were critical to the eradication of virus infection and future immunological surveillance to ensure the absence of infection. The recombinant protein produced as an antigen was a biotinylated fusion protein, a truncated capsid protein of the strain of MPV detected in the ARC, and was produced using the PinpointTM vector and with expression in Escherichia coli. The protein was produced as an insoluble intracellular product within inclusion bodies and was solubilised using urea and purified. The purified protein was utilised as an antigen for ELISA and the WIB assays to detect virus antibody in infected mice. The outbreak of MPV infection in the ARC was used as an unique opportunity for assessment of the seroprevalence of MPV-1 infection in a large laboratory mouse colony and to utilise this data to determine the sampling size needed to reliably detect MPV-1 infection within such large laboratory mouse colonies. An overall seroprevalence of 16.5% was detected using the developed serological tests, but considerable variation in prevalence was detected in different mouse strains. The response to MPV infection of 4 different but common strains of mice was determined as a basis for developing appropriate surveillance procedures and the selection of appropriate sentinel animals. The effect of infection of these strains at different ages was also investigated. Virus replication was detected in tissues of all the mice strains infected (outbred ARC(s) and inbred C57BL/6JArc, BALB/c and BALB/c-Foxn1nu/Arc) as juveniles and adults, with the exception of C57BL/6JArc inoculated as adults. However, while seroconversion in mice inoculated as juveniles and adults was detected in ARC(s) and C57BL/6JArc mice, it was not detected in BALB/c mice. The high rate of seroconversion to MPV, the early and prolonged development of an immune response, and the lack of age differences in their susceptibility indicated that ARC(s) mice would provide reliable sentinels for the detection of MPV. The genomic nucleotide sequence of the ARC strain, excluding the terminal palindromic regions and the predicted amino acid sequences of the non-structural and structural proteins was determined. This strain was very similar (98-99% nucleotide identity) to the previously described MPV strains MPV-1a, MPV-1b and MPV -1c. The similarity suggested there were unlikely to be significant antigenic differences in the proteins of the ARC strain and those strains of MPV reported previously.
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5

Migdalska, Anna Marta. "Modelling human genetic disorders in mice." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610341.

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6

Berting, Jennifer Irene. "Inbreeding effects on physiological responses to chronic hypoxia in mice (Mus musculus) /." Electronic version (PDF), 2007. http://dl.uncw.edu/etd/2007-3/bertingj/jenniferberting.pdf.

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7

Jyotika, Jigyasa. "Deletion of the Bax gene severely impairs sexual behavior and modestly impairs motor function in mice." Connect to this title, 2008. http://scholarworks.umass.edu/theses/158/.

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8

Wang, Bin. "Functional studies of QRF-1 /." Digital version accessible at:, 2000. http://wwwlib.umi.com/cr/utexas/main.

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9

Lai, Yeuk-yu. "Characterization of lymphocyte development in young and aged mice." Click to view the E-thesis via HKUTO, 2003. http://sunzi.lib.hku.hk/hkuto/record/B31971076.

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10

周燕華 and Yin-wah Eva Chow. "A study of spontaneously developing malignant lymphoma in SJL/N mice by immunoenzymatic methods." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1986. http://hub.hku.hk/bib/B31969549.

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11

Chow, Yin-wah Eva. "A study of spontaneously developing malignant lymphoma in SJL/N mice by immunoenzymatic methods." [Hong Kong : University of Hong Kong], 1986. http://sunzi.lib.hku.hk/hkuto/record.jsp?B12324541.

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12

Augustsson, Hanna. "Ethoexperimental studies of behaviour in wild and laboratory mice : risk assessment, emotional reactivity and animal welfare /." Uppsala : Dept. of Large Animal Clinical Sciences, Swedish Univ. of Agricultural Sciences, 2004. http://epsilon.slu.se/v174.pdf.

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13

Brown, Steven J. "Immunological studies of a glycosylation based mouse model of colitis /." Connect to thesis, 2004. http://eprints.unimelb.edu.au/archive/00000788.

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Thesis (Ph.D.)--University of Melbourne, Dept. of Gastroenterology and the Immunology Research Centre St. Vincents Hospital & Dept of Medicine, 2004.
Typescript (photocopy). Includes bibliographical references (leaves 309-343).
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14

歐穗欣 and Sui-yan Au. "Characterization of the mouse myosin va cargo-binding domain." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B31227107.

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15

Kwan, Tin-fu. "Lymphocyte development in collagen-induced arthritis mice." Click to view the E-thesis via HKUTO, 2003. http://sunzi.lib.hku.hk/hkuto/record/B31971064.

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16

關天富 and Tin-fu Kwan. "Lymphocyte development in collagen-induced arthritis mice." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31971064.

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17

黎若愚 and Yeuk-yu Lai. "Characterization of lymphocyte development in young and aged mice." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31971076.

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18

Iñiguez, Sergio Diaz. "The effects of acute posttraining injections of cocaine on spatial memory in C57BL/6 mice." CSUSB ScholarWorks, 2007. https://scholarworks.lib.csusb.edu/etd-project/3244.

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The purpose of this study was to investigate the effects of cocaine on spatial memory consolidation using the Morris water maze. Specifically, male and female C57BL/6 mice were trained on a spatial water task, and then administered a single posttraining injection of saline or cocaine (1.25, 2.5, 5.0, or 20.0 mg/kg).
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19

Li, Siu-ming Ian. "A study of cyclophosphamide on dextran sulfate sodium-induced ulcerative colitis in mice." Click to view the E-thesis via HKUTO, 2004. http://sunzi.lib.hku.hk/hkuto/record/B31971994.

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20

Yu, Chun-I. Palucka Karolina Banchereau Jacques. "Humanized mice to test vaccination against influenza virus via dendritic cells." Waco, Tex. : Baylor University, 2008. http://hdl.handle.net/2104/5184.

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Thesis (Ph.D.)--Baylor University, 2008.
In abstract the '2' and '-/-' in NOD-SCID-[beta]2m-/- is superscript. In abstract the '+' after CD34 and CD8 is superscript. In abstract the '-' and '+' in CD45RA-CD27+CD4+ are superscript. Includes bibliographical references (p. 103-123).
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21

Gliddon, Briony Lee. "Enzyme replacement therapy in a murine model of mucopolysaccharidosis type IIIA /." Title page, contents and abstract only, 2002. http://web4.library.adelaide.edu.au/theses/09PH/09phg5595.pdf.

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22

Stepp, Phillip W. "The effects of hypotyroidism, the acute inflammatory response, and caloric restriction on neurogensis and behavior in mice /." free to MU campus, to others for purchase, 2004. http://wwwlib.umi.com/cr/mo/fullcit?p3164543.

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23

Tan, Ju Chiat Graduate School of Biomedical Engineering Faculty of Engineering UNSW. "Investigation of abnormal cardiac function in murine models of hypocontractility and hypercontractility." Awarded by:University of New South Wales. Graduate School of Biomedical Engineering, 2006. http://handle.unsw.edu.au/1959.4/28879.

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Heart failure has a significant impact on mortality and morbidity. Dilated cardiomyopathy (DCM) is the third most common cause of heart failure and the most common reason for heart transplantation. Familial DCM is known to be caused by mutations in the LMNA gene encoding lamins A and C. New methods to enhance cardiac contractility would be beneficial in the treatment or prevention of heart failure. The focus of this thesis was to evaluate the mechanisms of altered contractility in two mouse models: the LMNA knockout model (homozygous, Lmna-/-; heterozygous, Lmna+/-) generated by targeted deletion of the lmna gene, and the model of enhanced contractility due to cardiac alpha1A-adrenergic receptor (???1A-AR) overexpression (A1A1). Previous studies have found altered nuclear-desmin connections in lamin A/C deficient mice. It was proposed that these alterations result in ???defective force transmission???, which leads to DCM. Studies in this thesis have supported this hypothesis. Studies of isolated single cardiomyocytes from mice aged 4-6 weeks demonstrated abnormal cell morphology and contractile dysfunction in Lmna-/- cardiomyocytes, while Lmna+/- cells showed no overt phenotype. Excitation-contraction coupling experiments and forcecalcium studies in skinned fibers excluded altered calcium kinetics as a primary cause of DCM in this model, but there was evidence of reduced sarcomere numbers and reduced sarcomere lengths as a contributor to reduce force generation in Lmna-/- and Lmna+/- mice. Previous in vivo studies showed that A1A1 mice had enhanced contractility with the absence of hypertrophy. Studies on isolated single cardiomyocytes from A1A1 mice aged 8-12 weeks showed reduced contractility in the absence of ???1A-AR stimulation, but an exaggerated response to ???1A-AR stimulation. In contrast isolated isovolumic Langendorff perfused A1A1 hearts without ???1A-AR stimulation replicated the enhanced contractility observed in vivo. These studies are consistent with down-regulation of contractility due to the hyperactivity of the overexpressed ???1A-AR in vivo, which only becomes evident in isolated cells without ???1A-AR stimulation due to the loss of functional receptor numbers during isolation. Sufficient spontaneously active ???1A-ARs are preserved in the isolated Langendorff heart preparation to ensure maximum contractility driven by increase calcium release.
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24

Naik, Shalin Hemant. "Distinct precursors of the dendritic cell subtypes /." Connect to thesis, 2006. http://eprints.unimelb.edu.au/archive/00001885.

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25

Tsang, Hon-man. "Studies of Mll-Een fusion gene in a conditional mouse model of human leukemia." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39558034.

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26

McEwen, Kirsten Rose. "Epigenetic regulation of imprinted loci in the mouse." Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609297.

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27

Wong, Ka-yan Karen, and 黃嘉欣. "The functional interaction of mouse secretin and angiotensin II receptors." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B46087187.

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28

Robertson, Kirsten 1975. "The reproductive phenotype of the male aromatase knockout mouse." Monash University, Dept. of Biochemistry and Molecular Biology, 2001. http://arrow.monash.edu.au/hdl/1959.1/8444.

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29

楊可儀 and Ho-yee Yeung. "Study on the function and regulation of stanniocalcin in mouse neuroblastoma cells." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31245043.

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30

Soler, David C. "The PP1 gamma isoforms restore spermatogenesis but not fertility in PP1 gamma null mice." [Kent, Ohio] : Kent State University, 2009. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=kent1259087463.

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Thesis (Ph.D.)--Kent State University, 2009.
Title from PDF t.p. (viewed May 17, 2010). Advisor: Srinivasan Vijayaraghavan. Keywords: sperm; spermatogenesis; PP1gamma2; PP1gamma1; mice; transgene. Includes bibliographical references (p. 102-123).
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31

McLean, Fiona Hamilton. "The impact of a high-fat diet on memory in mice." Thesis, University of Aberdeen, 2016. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=231763.

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Obesity and type II diabetes are associated with dementia and Alzheimer's disease. A high-fat diet induces memory deficits in rodents, however, complex episodic-like memory, has not been tested. Episodic memory is the recollection of events using a “what-where-when/which” experience and is the first memory to be compromised in Alzheimer's disease. To identify a link between a high-fat diet and episodic memory, 12 week old, male, C57Bl/6 mice, were fed a semi purified high-fat or low-fat diet ad libitum and tested with object-place-context (episodic-like), novel object recognition, object-place (spatial) and object-context (contextual) memory tasks for up to 2 weeks. A separate group of animals were fed a high-fat diet for 1 week followed by a low-fat diet for 1 week. Animals were killed after 3 days, 1 week or 2 weeks on diet. Brains were kept frozen until the hippocampus was dissected and proteomics performed. Further studies were carried out in rat primary hippocampal cell cultures to investigate the impact of different fatty acids on neuronal dendritic morphology. We found that episodic-like memory is compromised after only one day of a high fat diet together with spatial and contextual tasks. The ability to carry out the novel object recognition test remained intact. Proteomic analysis of hippocampal tissue revealed changes in a number of proteins associated with metabolism, cell stress, cell signalling, inflammation and the cytoskeleton. High-fat diet induced changes were reversed by a low-fat diet. Hippocampal neuron cultures showed that long chain saturated fatty acid palmitic acid, a component of the high-fat diet used in the behavioural and proteomic studies, caused reduced dendritic arborisation whist n-3 polyunsaturated fat docosahexaenoic acid negated these effects. These data link high-fat diet to indices of hippocampal neuronal damage and memory deficits and have implications for the link between diet, obesity and cognitive decline.
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32

Buonincontri, Guido. "Advanced MRI for cardiac assessment in mice." Thesis, University of Cambridge, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.648679.

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33

Li, Siu-ming Ian, and 李紹銘. "A study of cyclophosphamide on dextran sulfate sodium-induced ulcerative colitis in mice." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31971994.

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34

Slobedman, Barry. "Molecular analysis of herpes simplex virus type 1 latency in experimentally infected mice /." Title page, contents and abstract only, 1994. http://web4.library.adelaide.edu.au/theses/09PH/09phs634.pdf.

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Thesis (Ph. D.)--University of Adelaide, Dept. of Microbiology and Immunology, 1995?
Copies of author's previously published articles inserted inside back cover. Includes bibliographical references (leaves 137-179).
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35

Clifford, Adrianne Brown. "Tumor Associated Macrophages in a MaFIA Mouse Model." Diss., CLICK HERE for online access, 2006. http://contentdm.lib.byu.edu/ETD/image/etd1427.pdf.

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36

Li, Yuk-yin. "Adrenomedullin in the rat reproductive systems and the changes of the gene expression of adrenomedullin and its receptor components during ageing." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/HKUTO/record/B3955692X.

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37

Liu, Yan. "The immunosuppressive effects of Triptolide and Rapamycin on mouse model of cardiac transplantation." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/hkuto/record/B39793904.

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38

陳卓榮 and Cheuk-wing Wilson Chan. "Molecular basis for increased bone formation in a mouse expressing mutant collagen X." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31227132.

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39

Fisher, Rosie. "Utrophin in therapy of Duchenne muscular distrophy." Thesis, University of Oxford, 2001. http://ora.ox.ac.uk/objects/uuid:192fbccd-d037-4ce8-b1cd-0315afe1860d.

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40

Ross, Shelley 1973. "Generation and characterization of mice lacking the α4 nicotinic receptor subunit." Monash University, Dept. of Medicine, 2001. http://arrow.monash.edu.au/hdl/1959.1/9180.

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41

Xiang, Li. "Metabolomics study of regulatory effects of exercise training on db/db type 2 diabetic mice." HKBU Institutional Repository, 2018. https://repository.hkbu.edu.hk/etd_oa/489.

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Type 2 diabetes mellitus (T2DM) is mainly caused by genetic modifications and inappropriate life styles. The complexity of T2DM has brought us challenges for a comprehensive understanding of altered metabolic pathways that contributing to the development of T2DM. Therefore, a comprehensive metabolic analysis is needed. To date, taking regular exercise is a common and effective therapeutic way known to antagonize the metabolic disorders of T2DM. However, the regulatory effects of exercise on T2DM or T2DM induced complications have not been clearly characterized. Here, we present the effect of physical activity on biochemical changes in diabetic db/db mice in plasma, urine, skeletal muscle and kidney samples. Based on liquid chromatography coupled with high resolution Orbitrap mass spectrometry (LC-MS) and gas chromatography coupled with mass spectrometry (GC-MS), two major approaches, untargeted and targeted metabolomics studies, have been developed to delineate metabolic signatures in various kinds of biofluid and tissue samples. Targeted quantification methods on acylcarnitines and acyl-CoA have been developed. Untargeted metabolomics analysis by GC-MS and LC-MS have also been developed to draw a more comprehensive view of the metabolic changes in response to T2DM and exercise on db/db diabetic mice. The transcript expressions of mRNA in pathways of interest have also been measured to confirm the hypothesis. Firstly, a targeted quantification method of acylcarnitines by using high resolution parallel reaction monitoring (PRM) on LC-MS platform has been developed. A total of 117 acylcarnitines were detected from plasma and urine samples. The application of targeted profiling of acylcarnitines in db/m+ control and db/db diabetic mice indicated incomplete amino acid and fatty acid oxidation in diabetic mice. Interestingly, the reduction of medium odd-numbered chain acylcarnitines in urine samples was firstly observed between db/m+ and db/db mice. The high resolution PRM method makes it possible to monitor the widespread metabolic changes of the acylcarnitines in response to stimuli. Besides, the accurate MS and MS/MS spectra data of the 117 acylcarnitines could be used as mass spectrometric resources for the identification of acylcarnitines. In addition to targeted metabolomics analysis, untargeted metabolomics profiling analysis in plasma samples indicated that db/db diabetic mice may be more susceptible to exercise for energy expenditure. Interestingly, all the results from plasma, skeletal muscle and kidney samples may demonstrate that physical activity could mitigate insulin resistance in T2DM mice through improving fatty acid β-oxidation (FAO) and eliminating overloaded intermediate which contribute to insulin resistance. Specifically, the results from kidney samples demonstrated that exercise exhibit beneficial effect in reducing hyperlipidemia, expression levels of inflammatory markers (TNFα, IL-6 and COX2) and fibrosis markers (Collagen 1), and alleviating diabetic nephropathy (DN) induced mesangial expansion in kidneys of diabetic mice. The results of metabolic changes in kidney of db/db mice revealed that the accumulation of acyl-CoA, phospholipids and hydroxylated acylcarnitines were substantially ameliorated by exercise, and the reduction of important enzymes CTP1α and Acadl in FAO were partially reversed. In addition, branched-chain amino acids (BCAA) metabolism which positively related to inflammation (TNFα) was down-regulated in DN mice by exercise. What’s more, the accumulation of uric acid, which contributes to inflammation and tubulointestitial fibrosis in kidney disease, together with its six precursors have also been substantially reduced. The results in kidney samples demonstrated that in addition to beneficial effect in alleviating lipotoxicity through improving FAO efficiency, exercise also ameliorated diabetic induced inflammation and fibrosis via promoting BCAA catabolism and accelerating the elimination of uric acid. Together, the mass spectrometry-based metabolomics study is a powerful tool to investigate the regulatory effect of exercise on complex metabolic diseases. The results may provide informative insights into the underlying the mechanism of exercise on T2DM and T2DM induced complications.
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42

Alhindi, Yosra. "Effects of low citrate synthase activity on physiological responses of mice to high fat diet and palmitate induced lipotoxicity." Thesis, University of Aberdeen, 2016. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=231391.

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The main aim of this thesis was to examine the hypothesis that the A/J strain variant of H55N substitution affects citrate synthase (CS) enzyme activity and metabolic health in mice fed a high fat diet (HFD). C57BL/6J (B6) mice and congenic B6.A-(rs3676616-D10Utsw1)/KjnB6 (B6.A) mice, a strain which carries the A/J allele of Cs on the B6 strain background, were fed a HFD (45% kcal from fat) for 12 weeks. CS activity, but not that of ß-hydroxyacyl-coenzyme dehydrogenase was lower in the gastrocnemius muscle of B6.A mice compared to B6 mice (P< 0.001). During HFD feeding the glucose tolerance of mice decreased progressively and to a greater extent in B6.A females compared to B6 females, with males showing a similar trend. Interestingly, after 12 weeks of HFD feeding only B6.A males showed increases (P< 0.05) in their resting metabolic rate; moreover; core body temperature were also increased (P< 0.05) for congenic B6.A of both sexes by the end of the study. However, body weight and fat gain did not differ between B6.A and B6 mice. The second aim of the thesis was to test the hypothesis that low CS activity promotes palmitate-induced lipotoxicity in muscle cells. After 18 hours of incubation in 0.8 mM palmitate, C2C12 muscle cells with a ~50% reduction in CS activity showed low (P< 0.001) viability, increased (P< 0.001) levels of cleaved Caspase-3, high levels of AMP-activated protein kinase and acetyl-CoA carboxylase phosphorylation (P< 0.05), low levels of protein kinase B phosphorylation, high mitogen-activated protein kinases activation (P< 0.001) compared to the control shRNA cells. This was coupled with higher levels of mitochondrial proteins (P< 0.05), which are involved in oxidative phosphorylation. C2C12 cells with reduced CS activity also showed high reactive oxygen species production (P< 0.05), low intracellular ATP levels (P< 0.05), and lower basal mitochondrial respiration (P< 0.001). In summary, the A/J strain variant of H55N is associated with low CS enzyme activity and impaired metabolic health when fed HFD. Palmitate has a lipotoxic effect on Cs shRNA transfected cells and can lead to cell death.
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Duncan, Carlotta Clinical School St Vincent's Hospital Faculty of Medicine UNSW. "Molecular expression analyses of mice treated with antipsychotic drugs." Publisher:University of New South Wales. Clinical School - St Vincent's Hospital, 2008. http://handle.unsw.edu.au/1959.4/41239.

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Schizophrenia is a devastating psychiatric disorder that affects approximately 1% of the population. The main treatments for schizophrenia are antipsychotic drugs that target dopamine receptors, yet the underlying biological mechanisms through which they alleviate the symptoms of schizophrenia remain ill defined. In this study, we used microarray analysis to profile the expression changes of thousands of genes simultaneously, following antipsychotic drug treatment of mice. Mice were treated chronically (28 days), or for a novel intermediate time-point (7 days), with one of three antipsychotic drugs: clozapine, haloperidol or olanzapine. The use of three drugs enabled us to discern antipsychotic-specific effects co-regulated by multiple drugs, rather than the side effects of individual compounds. Transcript profiling and validation by quantitative PCR of whole brain tissue revealed antipsychotic drug regulation of genes in diverse biological pathways, including: dopamine metabolism, neuropeptide and second-messenger signalling, neurogenesis, synaptic plasticity, cell adhesion, myelination, and voltage-gated ion channels. The regulation of voltage-gated channels by antipsychotic drugs has been suggested previously by electrophysiological studies, although thorough analysis has not been undertaken in vivo. Therefore, the second aim of this study was to characterise the regional mRNA and protein expression of two genes altered by multiple APDs, the voltage-gated potassium channel ??-subunit (Kcna1) and voltage-gated potassium channel interacting protein (Kchip3). Regional characterisation and expression analyses were carried out by immunohistochemistry, in situ hybridisation, and Western blot analysis of mouse brain regions of interest to schizophrenia and its treatment. Following 7-day haloperidol treatment we observed up-regulation of Kcna1 in the striatum and dentate gyrus, with increased protein in the striatum, hippocampus and midbrain; and down-regulation of Kchip3 in the striatum, with decreased protein in the cortex, hippocampus and midbrain. These studies implicate voltage-gated potassium channels in the antipsychotic drug regulation of midbrain dopaminergic neuronal activity, adult neurogenesis and/or striatothalamic GABAergic neuronal inhibition. These findings indicate that regulation of potassium channels may underlie some of the mechanisms of action of antipsychotic drugs, and that voltage-gated ion channels may provide alternative drug targets for the treatment of schizophrenia.
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Wong, Chun-wai. "The fate of undifferentiated murine embryonic stem cells in a mouse model with acute myocardial infarction." Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B31927634.

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Masters, Seth Lucian. "The role of the SPRY domain in the SPRY domain containing SOCS box proteins (SSBs) /." Connect to thesis, 2005. http://eprints.unimelb.edu.au/archive/00001571.

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Thesis (Ph.D.)--University of Melbourne, The Walter and Eliza Hall Institute of Medical Research, Division of Cancer and Haematology, Dept. of Medical Biology,Faculty of Medicine,Dentistry and Health Sciences, 2006.
Typescript. Includes bibliographical references (leaves 192-210).
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Cheung, Kwok-ho Alvin. "Genetic and pharmacological approaches to study the role of the polyol pathway enzymes in diabetic and ischemic retinopathy." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39558617.

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Yang, Fan. "Intervertebral disc regeneration using mesenchymal stem cells a mouse model study /." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39556979.

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Tang, Wai-ho Jack. "Polyol pathway contributes to iron-induced oxidative damage in ischemia-reperfused rat hearts." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/HKUTO/record/B39558022.

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曾漢文 and Hon-man Tsang. "Studies of Mll-Een fusion gene in a conditional mouse model of human leukemia." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B39558034.

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Liu, Yan, and 劉艷. "The immunosuppressive effects of Triptolide and Rapamycin on mouse model of cardiac transplantation." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B39793904.

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