Relevant bibliographies by topics / Mice as laboratory animals Fertility

Academic literature on the topic 'Mice as laboratory animals Fertility'

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Published: 10 December 2022
Last updated: 29 January 2023

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Journal articles on the topic "Mice as laboratory animals Fertility"

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Hardy, C. M., J. F. M. ten Have, K. J. Mobbs, and L. A. Hinds. "Assessment of the immunocontraceptive effect of a zona pellucida 3 peptide antigen in wild mice." Reproduction, Fertility and Development 14, no. 3 (2002): 151. http://dx.doi.org/10.1071/rd01112.

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Immunizing laboratory mice against a short peptide to mouse zona pellucida protein 3 (mZP3; amino acids 328-342) reduces fertility in some strains. This antigen was therefore tested to see if it is suitable for use in an immunocontraceptive vaccine to control wild mice. Mouse zona pellucida protein 3 peptide conjugated to a carrier protein (keyhole limpet hemocyanin) was considerably more immunogenic and effective in reducing fertility in wild mice when compared with inbred BALB/c mice. Fertility of the immunized wild mice was reduced by over 50% compared with controls, whereas BALB/c mice showed no reduction. Variation in the responses between individual animals to mZP3 peptide was observed and infertility correlated to the presence of cross-reacting antibodies to native zona pellucida in wild, but not BALB/c, mice.
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Gavora, J. S., B. Benkel, H. Sasada, W. J. Cantwell, P. Fiser, R. M. Teather, J. Nagai, and M. P. Sabour. "An attempt at sperm-mediated gene transfer in mice and chickens." Canadian Journal of Animal Science 71, no. 2 (June 1, 1991): 287–91. http://dx.doi.org/10.4141/cjas91-037.

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Experiments were carried out to transform laboratory mice and domestic chickens by use of sperm incubated with bacterial plasmid DNA. Following demonstration of "uptake" of such DNA by sperm of both species, attempts were made to replicate a previously published procedure (Lavitrano et al. 1989, Cell 57: 717–723) for producing transgenic mice through in vitro fertilization (IVF). Also, female mice and hens were inseminated (AI) with sperm which had been incubated in a DNA solution. Such incubation did not influence the fertility or hatchability of the hens' eggs. However, no transformed progeny were detected among 45 mice produced by IVF or among 69 mice and 470 chickens produced by AI. Key words: Sperm-mediated DNA transfer, mice, chickens
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Sitasiwi, Agung Janika, Sri Isdadiyanto, and Siti Muflichatun Mardiati. "Pelacakan eskpresi protein pada testis mencit (Mus musculus) setelah paparan esktrak etanol daun mimba (Azadirachta indica)." Jurnal Sain Veteriner 37, no. 1 (August 5, 2019): 90. http://dx.doi.org/10.22146/jsv.43027.

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Abstract Azadirachta indica (Neem) has been shown to affect the fertility of mice by interfering with the synthesis of testosterone in mice. The aim of this study was to detect the testes protein expression of mice after exposure to the ethanolic Neem leaf extract. The laboratory animals of this study were 20 male Swiss Webster mice with three months in age and body weight ranging from 27.5 grams. The mice were divided into two treatment groups, namely K (control group, exposed with distilled water) and P (treatment group, exposed to etahnolic Neem leaf extract with 14 mg/animal /day). The treated were given for 21 days and the testicular protein was carried out on the 22nd day. The variables observed were testes weight, concentration and expression of proteins isolated from the testes. The protein concentration is determined by a spectrophotometer at a wavelength of 450nm. The protein expression was observed and determined based on the results of protein electrophoresis (SDS-PAGE). The results showed that protein expression in the treatment group has a lower concentration compared to the control group. Those results was confirmed by thinner bands in SDS-PAGE result. Those proteins thought to be a fertility determinant in mammals. Keywords : anti-fertility; Neem; protein expression Abstrak Azadirachta indica (Mimba) telah terbukti mempengaruhi fertilitas mencit dengan cara mengganggu sintesis hormon testoteron pada mencit. Penelitian ini bertujuan untuk melacak ekspresi protein pada testis mencit setelah paparan ekstrak etanol daun Mimba. Hewan uji penelitian ini adalah 20 ekor mencit Swiss Webster jantan dengan umur tiga bulan dan bobot badan berkisar 27.5 gram. Hewan uji dibagi menjadi 2 kelompok perlakuan, yaitu K (kelompok kontrol, dipapar akuades) dan P (kelompok perlakuan, dipapar dengan ekstrak etanol daun Mimba dengan dosis 14 mg/ekor/hari). Pemberian bahan uji dilakukan secara oral selama 21 hari. Variabel yang diamati adalah bobot testes, konsentrasi serta eskpresi protein yang diisolasi dari testis. Isolasi protein testis dilakukan pada hari ke-22. Konsentrasi protein ditentukan dengan spectrofotometer pada panjang gelombang 450nm. Ekspresi protein diamati dan ditentukan berdasar hasil elektroforesa protein. Hasil penelitian menunjukkan bahwa ekspresi protein pada kelompok perlakuan menunjukkan konsentrasi yang lebih rendah dengan pita yang lebih tipis jika dibandingkan dengan kelompok kontrol. Kesimpulan penelitian ini adalah paparan ekstrak etanol daun Nimba menyebabkan gangguan ekspresi protein yang diduga berperan dalam menentukan fertilitas mamalia. Kata kunci : Mimba; anti-fertilitas; eskpresi protein;
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4

Hamad, Karim R. "Effect of Fenugreek Seeds in Maternal Diet on Some Features of Newborn Pups in Albino Mice." Polytechnic Journal 11, no. 2 (December 30, 2021): 42–47. http://dx.doi.org/10.25156/ptj.v11n2y2021.pp42-47.

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Introduction: Fenugreek seeds have got interest of researchers for their positive effects on laboratory animals and human being, including their effects on reproductive system. The present study aimed to investigate the effects of 5% raw fenugreek seed and its boiled aqueous extract containing diet on adult female mice, for 2 weeks before mating, to evaluate fertility, and some features of newborn pups. Materials and Methods: A total of 90 adult female mice randomly and equally were divided into three groups. Group I: Control, Group II: Female mice treated with 5% raw fenugreek seed containing diet, and Group III: Female mice treated with boiled aqueous extract of 5% fenugreek seed containing diet. The animals were treated daily for 2 weeks before mating. Some of them were mated with normal males, while the others were used for uterus study. The following parameters were evaluated: Fertility of adult female mice; body weight (BW); uterus weight; uterus sections; litter size; sex ratio; live and dead pups; and pup BW. Results: There was non-significant change in litter size and sex ratio; live and dead pups; adult female BW; and uterus weight. While wet and dry weight of pups, were significantly increased. Histological sections in uterus showed multilayering in endometrial glands of both the experimental groups. No differences were observed between fenugreek treated groups. Conclusion: Both forms of fenugreek seed (raw and aqueous extract) induced significant increase in pup weight; possibly are due to multilayering in endometrial glands of uterus.
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Vigueras-Villaseñor, Rosa María, Martín Alejandro Fuentes-Cano, Margarita Chávez Saldaña, Liliana Rivera Espinosa, Rafael Reynoso-Robles, Patricia Rojas, Pilar Durán, and Julio César Rojas-Castañeda. "Fetal and Postnatal Nicotine Exposure Modifies Maturation of Gonocytes to Spermatogonia in Mice." Analytical Cellular Pathology 2020 (December 15, 2020): 1–14. http://dx.doi.org/10.1155/2020/8892217.

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Studies in laboratory animals have shown that male offspring from dams, exposed to nicotine during pregnancy and postnatal periods, show alterations in fertility, although the origin of this is still uncertain. In this study, we examined in a mouse model if the process of gonocyte maturation to spermatogonia was affected in male offspring from dams with nicotine administration during pregnancy and postnatal periods. BALB/C mice, with and without nicotine administrations in pregnancy and postnatal periods, were studied. The animals were euthanized at 3, 7, 10, 16, and 35 days postpartum (dpp). Testicular tissue samples were processed for histological, ultrastructural, and immunohistochemical studies; and testicular lipoperoxidation was determined. It was observed that in the nicotine-exposed animals, there was increased apoptosis and a reduction in the number of gonocytes that matured to spermatogonia. This gonocyte-spermatogonia maturation reduction was associated with a greater immunoreactivity to nicotinic acetylcholine receptors in the germ cells. Lipoperoxidation was similar in both groups until 16 dpp, with significant reduction at 35 dpp. Our findings suggest that nicotine intake during pregnancy and postnatal periods can affect the process of maturation of gonocytes to spermatogonia and the pool of available spermatogonia for spermatogenesis.
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Tello, Javier A., Trudy Kohout, Rafael Pineda, Richard A. Maki, R. Scott Struthers, and Robert P. Millar. "Reproductive physiology of a humanized GnRH receptor mouse model: application in evaluation of human-specific analogs." American Journal of Physiology-Endocrinology and Metabolism 305, no. 1 (July 1, 2013): E67—E77. http://dx.doi.org/10.1152/ajpendo.00624.2012.

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The human GnRH receptor (GNRHR1) has a specific set of properties with physiological and pharmacological influences not appropriately modeled in laboratory animals or cell-based systems. To address this deficiency, we have generated human GNRHR1 knock-in mice and described their reproductive phenotype. Measurement of pituitary GNRHR1 transcripts from homozygous human GNRHR1 knock-in ( ki/ ki) mice revealed a severe reduction (7- to 8-fold) compared with the mouse Gnrhr1 in wild-type mice. 125I-GnRH binding assays on pituitary membrane fractions corroborated reduced human GNRHR1 protein expression in ki/ ki mice, as occurs with transfection of human GNRHR1 in cell lines. Female homozygous knock-in mice displayed normal pubertal onset, indicating that a large reduction in GNRHR1 expression is sufficient for this process. However, ki/ ki females exhibited periods of prolonged estrous and/or metestrous and reduced fertility. No impairment was found in reproductive maturity or adult fertility in male ki/ ki mice. Interestingly, the serum LH response to GnRH challenge was reduced in both knock-in males and females, indicating a reduced GNRHR1 signaling capacity. Small molecules targeting human GPCRs usually have poor activities at homologous rodent receptors, thus limiting their use in preclinical development. Therefore, we tested a human-specific GnRH1 antagonist, NBI-42902, in our mouse model and demonstrated abrogation of a GnRH1-induced serum LH rise in ki/ ki mice and an absence of effect in littermates expressing the wild-type murine receptor. This novel model provides the opportunity to study the human receptor in vivo and for screening the activity of human-specific GnRH analogs.
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I’tishom, Reny, Doddy M. Soebadi, Aucky Hinting, Hamdani Lunardhi, and Rina Yudiwati. "IN VITRO FERTILITY TEST OF HUMAN SPERMATOZOA MEMBRANE PROTEIN FERTILIN BETA ANTIBODY IN MICE (Mus musculus Balb/c) AS IMMUNOCONTRACEPTIVE CANDIDATE." Folia Medica Indonesiana 52, no. 3 (August 14, 2017): 209. http://dx.doi.org/10.20473/fmi.v52i3.5453.

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One of the materials as potential candidates immunocontraception material is spermatozoa. Fertilin beta is spermatozoa membrane protein and is found only in mature spermatozoa and ejaculate, which serves as an adhesion molecule. Spermatozoa membrane protein that is used as an ingredient immunocontraception candidate, must have specific criteria that the specificity of spermatozoa, the role of antigen in the fertilization process, which includes the formation of immunogenicity sufficient antibody response has the potential to block fertilization. Antibodies against spermatozoa affect the stages before fertilization of the reproductive process and can hinder the development of the embryo after fertilization. Until now very little research data spermatozoa membrane protein as an ingredient immunocontraception are up to the test of experimental animals. The research objective is to prove the role of the resulting antibody induction of antibodies fertilin beta protein in the membrane of human spermatozoa induce agglutination and reduce motility thus reducing the number of in vitro fertilization. Research conducted at the IVF Laboratory, Department of Biology of Medicine, Faculty of Medicine, University of Airlangga. This research includes: Test the potential of antibody protein beta fertilin membrane of human spermatozoa and inhibit the role of antibodies in vitro fertilization in mice (Mus musculus Balb/c). In vitro studies have resulted in fertilization figure of 25% is smaller than the number that is equal to control fertilization of 58.7%, whereas previously the spermatozoa were incubated first with a beta membrane protein antibody fertilin human spermatozoa. While the percentage of inhibition of sperm to fertilize an oocyte by 33.75%. Potential imunokontraseptif considered effective if it decreased significantly (P <0.05) than the numbers fertilization in the treatment group compared with the control group. This shows fertilin beta membrane protein antibody has the ability to inhibit human spermatozoa to fertilize oocytes that reduce the number of fertilization.
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Zolotova, Natalia, Anna Kosyreva, Dzhuliia Dzhalilova, Nikolai Fokichev, and Olga Makarova. "Harmful effects of the microplastic pollution on animal health: a literature review." PeerJ 10 (June 14, 2022): e13503. http://dx.doi.org/10.7717/peerj.13503.

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Background The environmental pollution by microplastics is a global problem arising from the extensive production and use of plastics. Small particles of different plastics, measured less than 5 mm in diameter, are found in water, air, soil, and various living organisms around the globe. Humans constantly inhale and ingest these particles. The associated health risks raise major concerns and require dedicated evaluation. Objectives In this review we systematize and summarize the effects of microplastics on the health of different animals. The article would be of interest to ecologists, experimental biologists, environmental physicians, and all those concerned with anthropogenic environmental changes. Methodology We searched PubMed and Scopus from the period of 01/2010 to 09/2021 for peer-reviewed scientific publications focused on (1) environmental pollution with microplastics; (2) uptake of microplastics by humans; and (3) the impact of microplastics on animal health. Results The number of published studies considering the effects of microplastic particles on aquatic organisms is considerable. In aquatic invertebrates, microplastics cause a decline in feeding behavior and fertility, slow down larval growth and development, increase oxygen consumption, and stimulate the production of reactive oxygen species. In fish, the microplastics may cause structural damage to the intestine, liver, gills, and brain, while affecting metabolic balance, behavior, and fertility; the degree of these harmful effects depends on the particle sizes and doses, as well as the exposure parameters. The corresponding data for terrestrial mammals are less abundant: only 30 papers found in PubMed and Scopus deal with the effects of microplastics in laboratory mice and rats; remarkably, about half of these papers were published in 2021, indicating the growing interest of the scientific community in this issue. The studies demonstrate that in mice and rats microplastics may also cause biochemical and structural damage with noticeable dysfunctions of the intestine, liver, and excretory and reproductive systems. Conclusions Microplastics pollute the seas and negatively affect the health of aquatic organisms. The data obtained in laboratory mice and rats suggest a profound negative influence of microplastics on human health. However, given significant variation in plastic types, particle sizes, doses, models, and modes of administration, the available experimental data are still fragmentary and controversial.
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Vazifedust, Soheil, Hadi E. G. Ghaleh, and Farshad N. Aslabani. "Evaluation of the protective effect of coenzyme Q10 on the growth process of embryos from in vitro fertilization in laboratory mice treated with cyclophosphamide." Romanian Journal of Military Medicine 125, no. 2 (May 1, 2022): 230–36. http://dx.doi.org/10.55453/rjmm.2022.125.2.9.

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"Cyclophosphamide is an anticancer drug that acts as an alkylating agent after metabolism in the liver. Despite its many clinical applications in cancer treatment, this drug has toxic effects on the body's organs, especially the genitals. One of the most critical side effects is a change in the function of the female reproductive system, which can lead to infertility. This study aimed to investigate the antioxidant effects of coenzyme Q10 on cyclophosphamide-induced toxicity in vitro fertilized embryos in mice. In this experimental study, 24 female mice weighing 25 g 4 groups of 6 were divided and treated for 21 days. The first group (control group), solvent (cyclophosphamide) including DMSO and PBS (0.1 ml intraperitoneally), the second group (sham group), cyclophosphamide at a dose of 10 mg/kg was injected as a single dose, and the third group (experimental group), along with single-dose cyclophosphamide, coenzyme Q10 at a dose of 200 mg/kg/day was injected intraperitoneally and the fourth group (positive control group), only coenzyme Q10 at a dose of 200 mg/kg/day was injected intraperitoneally. At the end of the treatment period, ovulation stimulation was performed using PMSG and HCG hormones. Six adult male mice were used to prepare normal sperm. The animals were facilitated after anesthesia. After extraction of regular eggs and sperm and fertilization in HTF + 4 mg BSA medium, the fertilized eggs were incubated for 120 hours, and the embryonic developmental stages were examined during this period. Were analyzed by ANOVA and comparison of ratios. Cyclophosphamide significantly reduced oocyte quality, fertilization rate, pre-implantation embryonic development, and embryo quality. Coenzyme Q10 (Co Q10) significantly reduced the adverse effects of cyclophosphamide. The present study showed that crocin could protect the fertility of the female sex against damage caused by cyclophosphamide. "
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Shellam, GR. "The potential of murine cytomegalovirus as a viral vector for immunocontraception." Reproduction, Fertility and Development 6, no. 3 (1994): 401. http://dx.doi.org/10.1071/rd9940401.

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Wild populations of house mice (Mus domesticus) regularly undergo population eruptions in the cereal growing regions of S.E. Australia, causing significant damage to crops. Rodenticides are costly and are of limited usefulness, and the need for a biological control agent is widely recognized. The options for the use of an infectious agent to control mouse populations are considered. The three main types are: infectious agents which establish lethal infection; those which directly interfere with fertility; and recombinant virus vectors encoding fertility-associated proteins such as zona pellucida or sperm antigens in order to induce immunocontraceptive responses in infected mice. Ectromelia, a murine pox virus, has the potential for reducing mouse populations by lethal infection but it is not present in wild mice in Australia. The disadvantages of using ectromelia are that it would pose a significant threat to colonies of laboratory mice, there appears to be substantial innate resistance in Australian wild mice and it may not be entirely mouse-specific, thus placing native rodents at risk. A number of factors influencing the selection of a virus as a vector for immunocontraception are discussed. The mouse-specific murine cytomegalovirus (MCMV) fits most of these criteria. Infection with MCMV is already widespread in Australia with 80-90% of Mus domesticus tested being seropositive. It is a large DNA virus which establishes persistent, non-lethal infection, it is a suitable vector for the insertion of foreign genes and has a number of properties, including the capacity for superinfection, that should assist the recombinant virus to persist in wild mouse populations and induce an immunocontraceptive effect.
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Dissertations / Theses on the topic "Mice as laboratory animals Fertility"

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Agarwal, Rajat. "A model for minimizing cost for housing laboratory mice." [Gainesville, Fla.] : University of Florida, 2003. http://purl.fcla.edu/fcla/etd/UFE0001241.

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Hsu, Charlie Chun. "Isolation, characterization, and diagnosis of murine noroviruses, a newly recognized pathogen of mice." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4790.

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Thesis (Ph. D.)--University of Missouri-Columbia, 2007.
"December 2007" The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Vita. Includes bibliographical references.
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Filipovska-Naumovska, Emilija. "Development of methods for detection and eradication of mouse parvovirus from a laboratory mouse colony." Thesis, Filipovska-Naumovska, Emilija (2007) Development of methods for detection and eradication of mouse parvovirus from a laboratory mouse colony. PhD thesis, Murdoch University, 2007. https://researchrepository.murdoch.edu.au/id/eprint/676/.

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The mouse parvovirus designated MPV can infect laboratory mice and affect the humoral and cellular immune response of infected mice, reducing their value for biomedical and medical research. The development and maintenance of MPV-free mouse colonies for biomedical research is therefore essential and requires routine monitoring of the infection status of mice, using serological surveillance procedures. Recent experience in the Animal Resources Centre (ARC), a major supplier of mice to the medical research community in Australia, was that MPV infection was present but was not detectable with the serological tests that were then in routine use. This thesis reports the development of a polymerase chain reaction (PCR) assay for the detection of the MPV in the ARC mouse colonies, the genetic characteristics of the strain of MPV detected, the development of a recombinant virus protein that provided a suitable antigen for enzyme-linked immunosorbent assay (ELISA) and a Western immunoblot (WIB) assay for the detection of MPV antibodies, and use of these various assays to determine aspects of the epidemiology and pathogenicity of the infection that were critical to the eradication of virus infection and future immunological surveillance to ensure the absence of infection. The recombinant protein produced as an antigen was a biotinylated fusion protein, a truncated capsid protein of the strain of MPV detected in the ARC, and was produced using the PinpointTM vector and with expression in Escherichia coli. The protein was produced as an insoluble intracellular product within inclusion bodies and was solubilised using urea and purified. The purified protein was utilised as an antigen for ELISA and the WIB assays to detect virus antibody in infected mice. The outbreak of MPV infection in the ARC was used as an unique opportunity for assessment of the seroprevalence of MPV-1 infection in a large laboratory mouse colony and to utilise this data to determine the sampling size needed to reliably detect MPV-1 infection within such large laboratory mouse colonies. An overall seroprevalence of 16.5% was detected using the developed serological tests, but considerable variation in prevalence was detected in different mouse strains. The response to MPV infection of 4 different but common strains of mice was determined as a basis for developing appropriate surveillance procedures and the selection of appropriate sentinel animals. The effect of infection of these strains at different ages was also investigated. Virus replication was detected in tissues of all the mice strains infected (outbred ARC(s) and inbred C57BL/6JArc, BALB/c and BALB/c-Foxn1nu/Arc) as juveniles and adults, with the exception of C57BL/6JArc inoculated as adults. However, while seroconversion in mice inoculated as juveniles and adults was detected in ARC(s) and C57BL/6JArc mice, it was not detected in BALB/c mice. The high rate of seroconversion to MPV, the early and prolonged development of an immune response, and the lack of age differences in their susceptibility indicated that ARC(s) mice would provide reliable sentinels for the detection of MPV. The genomic nucleotide sequence of the ARC strain, excluding the terminal palindromic regions and the predicted amino acid sequences of the non-structural and structural proteins was determined. This strain was very similar (98-99% nucleotide identity) to the previously described MPV strains MPV-1a, MPV-1b and MPV -1c. The similarity suggested there were unlikely to be significant antigenic differences in the proteins of the ARC strain and those strains of MPV reported previously.
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Filipovska-Naumovska, Emilija. "Development of methods for detection and eradication of mouse parvovirus from a laboratory mouse colony." Filipovska-Naumovska, Emilija (2007) Development of methods for detection and eradication of mouse parvovirus from a laboratory mouse colony. PhD thesis, Murdoch University, 2007. http://researchrepository.murdoch.edu.au/676/.

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The mouse parvovirus designated MPV can infect laboratory mice and affect the humoral and cellular immune response of infected mice, reducing their value for biomedical and medical research. The development and maintenance of MPV-free mouse colonies for biomedical research is therefore essential and requires routine monitoring of the infection status of mice, using serological surveillance procedures. Recent experience in the Animal Resources Centre (ARC), a major supplier of mice to the medical research community in Australia, was that MPV infection was present but was not detectable with the serological tests that were then in routine use. This thesis reports the development of a polymerase chain reaction (PCR) assay for the detection of the MPV in the ARC mouse colonies, the genetic characteristics of the strain of MPV detected, the development of a recombinant virus protein that provided a suitable antigen for enzyme-linked immunosorbent assay (ELISA) and a Western immunoblot (WIB) assay for the detection of MPV antibodies, and use of these various assays to determine aspects of the epidemiology and pathogenicity of the infection that were critical to the eradication of virus infection and future immunological surveillance to ensure the absence of infection. The recombinant protein produced as an antigen was a biotinylated fusion protein, a truncated capsid protein of the strain of MPV detected in the ARC, and was produced using the PinpointTM vector and with expression in Escherichia coli. The protein was produced as an insoluble intracellular product within inclusion bodies and was solubilised using urea and purified. The purified protein was utilised as an antigen for ELISA and the WIB assays to detect virus antibody in infected mice. The outbreak of MPV infection in the ARC was used as an unique opportunity for assessment of the seroprevalence of MPV-1 infection in a large laboratory mouse colony and to utilise this data to determine the sampling size needed to reliably detect MPV-1 infection within such large laboratory mouse colonies. An overall seroprevalence of 16.5% was detected using the developed serological tests, but considerable variation in prevalence was detected in different mouse strains. The response to MPV infection of 4 different but common strains of mice was determined as a basis for developing appropriate surveillance procedures and the selection of appropriate sentinel animals. The effect of infection of these strains at different ages was also investigated. Virus replication was detected in tissues of all the mice strains infected (outbred ARC(s) and inbred C57BL/6JArc, BALB/c and BALB/c-Foxn1nu/Arc) as juveniles and adults, with the exception of C57BL/6JArc inoculated as adults. However, while seroconversion in mice inoculated as juveniles and adults was detected in ARC(s) and C57BL/6JArc mice, it was not detected in BALB/c mice. The high rate of seroconversion to MPV, the early and prolonged development of an immune response, and the lack of age differences in their susceptibility indicated that ARC(s) mice would provide reliable sentinels for the detection of MPV. The genomic nucleotide sequence of the ARC strain, excluding the terminal palindromic regions and the predicted amino acid sequences of the non-structural and structural proteins was determined. This strain was very similar (98-99% nucleotide identity) to the previously described MPV strains MPV-1a, MPV-1b and MPV -1c. The similarity suggested there were unlikely to be significant antigenic differences in the proteins of the ARC strain and those strains of MPV reported previously.
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Migdalska, Anna Marta. "Modelling human genetic disorders in mice." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610341.

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Berting, Jennifer Irene. "Inbreeding effects on physiological responses to chronic hypoxia in mice (Mus musculus) /." Electronic version (PDF), 2007. http://dl.uncw.edu/etd/2007-3/bertingj/jenniferberting.pdf.

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Jyotika, Jigyasa. "Deletion of the Bax gene severely impairs sexual behavior and modestly impairs motor function in mice." Connect to this title, 2008. http://scholarworks.umass.edu/theses/158/.

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Wang, Bin. "Functional studies of QRF-1 /." Digital version accessible at:, 2000. http://wwwlib.umi.com/cr/utexas/main.

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Lai, Yeuk-yu. "Characterization of lymphocyte development in young and aged mice." Click to view the E-thesis via HKUTO, 2003. http://sunzi.lib.hku.hk/hkuto/record/B31971076.

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周燕華 and Yin-wah Eva Chow. "A study of spontaneously developing malignant lymphoma in SJL/N mice by immunoenzymatic methods." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1986. http://hub.hku.hk/bib/B31969549.

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Books on the topic "Mice as laboratory animals Fertility"

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Peggy, Danneman, and Brayton Cory, eds. The laboratory mouse. Boca Raton: CRC Press, 2001.

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M, Brown Stephen D., Lyon Mary F, Rastan Sohaila, and International Committee on Standardized Genetic Nomenclature for Mice., eds. Genetic variants and strains of the laboratory mouse. 3rd ed. Oxford: Oxford University Press, 1996.

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Inoue, T., Tetsuo Noda, and Akio Nomoto. Hitogata moderu dōbutsu. Tōkyō: Shupuringā Fearāku Tōkyō, 2002.

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Janet, Rossant, and Tam Patrick P. L, eds. Mouse development: Patterning, morphogenesis, and organogenesis. San Diego: Academic Press, 2002.

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EMBO Workshop (1989 Basel Institute for Immunology). The Scid mouse: Characterization and potential uses :EMBO Workshop held at the Basel Institute for Immunology, Basel, Switzerland, February 20-22, 1989. Berlin: Springer-Verlag, 1989.

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Voipio, Hanna-Marja. Hiiren ja rotan tartuntataudit. Kuopio: Valtakunnallinen koe-eläinkeskus, Kuopion yliopisto, 1988.

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Making mice: Standardizing animals for American biomedical research, 1900-1955. Princeton, N.J: Princeton University Press, 2004.

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Rader, Karen A. Making mice: Standardizing animals for American biomedical research, 1900-1955. Princeton, N.J: Princeton University Press, 2004.

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Laboratory mouse procedural techniques: Manual and DVD. Boca Raton: CRC Press, 2011.

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Unger, Erik. Proteoglycans in diabetes. Uppsala: Sveriges Lantbruksuniversitet, 1991.

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Book chapters on the topic "Mice as laboratory animals Fertility"

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MacLellan, Aileen, Aimée Adcock, and Georgia Mason. "Behavioral Biology of Mice." In Behavioral Biology of Laboratory Animals, 89–111. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9780429019517-8.

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Baumans, Vera. "The welfare of laboratory mice." In The Welfare of Laboratory Animals, 119–52. Dordrecht: Springer Netherlands, 2007. http://dx.doi.org/10.1007/978-1-4020-2271-5_7.

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Pritchett-Corning, Kathleen R., and Christina Winnicker. "Behavioral Biology of Deer and White-Footed Mice, Mongolian Gerbils, and Prairie and Meadow Voles." In Behavioral Biology of Laboratory Animals, 147–63. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9780429019517-11.

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Geise, W. "Guidelines for the Use and Care of Small Laboratory Animals in Transplantation Research." In Organtransplantation in Rats and Mice, 27–40. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-72140-3_4.

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"Pathogens of Rats and Mice." In Natural Pathogens of Laboratory Animals, 19–107. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817824.ch2.

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de Boer, P., and J. H. de Jong. "Chromosome Pairing and Fertility in Mice." In Fertility and Chromosome Pairing: Recent Studies in Plants and Animals, 37–76. CRC Press, 2020. http://dx.doi.org/10.1201/9781003068433-3.

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Würbel, Hanno, Charlotte Burn, and Naomi Latham. "The behaviour of laboratory mice and rats." In The ethology of domestic animals: an introductory text, modular texts, 217–33. 2nd ed. CABI, 2009. http://dx.doi.org/10.1079/9781845935368.0217.

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Gil da Costa, Rui M., António Ramos Silva, Ana Faustino Rocha, Paula Alexandra Oliveira, Joaquim Gabriel, Ana Margarida Abrantes, and Maria Filomena Botelho. "Thermography in Animal Models of Cancer." In Innovative Research in Thermal Imaging for Biology and Medicine, 237–63. IGI Global, 2017. http://dx.doi.org/10.4018/978-1-5225-2072-6.ch011.

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Laboratory animals provide important models for studying human diseases, including many types of cancer. Mice are among the most commonly used laboratory animals, allowing for the study of carcinogenic agents, cancer development and for testing innovative preventive and therapeutic strategies. Thus, monitoring angiogenesis in animal models is a major goal for cancer research. Among the currently available imaging techniques, thermography is a useful approach for studying the superficial vascularization of cancer, based on their heat emissions. At this chapter emphasis is placed on thermography and its applications on laboratory animals, in comparison with other available and applicable imaging techniques. In conclusion, thermography may be usefully applied to the study of cancer vascularization in animal models, particularly when using laboratory rodents such as mice. Care is needed in adapting existing approaches to the specificities of each animal species.
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Gil da Costa, Rui M., António Ramos Silva, Ana Faustino Rocha, Paula Alexandra Oliveira, Joaquim Gabriel, Ana Margarida Abrantes, and Maria Filomena Botelho. "Thermography in Animal Models of Cancer." In Veterinary Science, 132–58. IGI Global, 2018. http://dx.doi.org/10.4018/978-1-5225-5640-4.ch007.

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Laboratory animals provide important models for studying human diseases, including many types of cancer. Mice are among the most commonly used laboratory animals, allowing for the study of carcinogenic agents, cancer development and for testing innovative preventive and therapeutic strategies. Thus, monitoring angiogenesis in animal models is a major goal for cancer research. Among the currently available imaging techniques, thermography is a useful approach for studying the superficial vascularization of cancer, based on their heat emissions. At this chapter emphasis is placed on thermography and its applications on laboratory animals, in comparison with other available and applicable imaging techniques. In conclusion, thermography may be usefully applied to the study of cancer vascularization in animal models, particularly when using laboratory rodents such as mice. Care is needed in adapting existing approaches to the specificities of each animal species.
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Rose, Nikolas, and Joelle M. Abi-Rached. "What’s Wrong with Their Mice?" In Neuro. Princeton University Press, 2013. http://dx.doi.org/10.23943/princeton/9780691149608.003.0004.

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This chapter discusses the use of animals to explore issues relating to human cognition, emotion, volition, and their pathologies. Researchers who use animal models in their work point to similarities in the genomes of the two species, in the structure of mouse and human brain, in patterns of brain activation, in neural mechanisms at the cellular and molecular level, in responses to drugs and so forth, perhaps with reference to evolution and the principle of conservation across species when it comes to the most basic aspects of living organisms, including their brains. The chapter then examines four interconnected themes: the question of the artificiality of the laboratory situation within which animal experiments are conducted; the idea of a model in behavioral and psychiatric research; the specificity of the human and the elision of history and human sociality; and the problem of translation.
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Conference papers on the topic "Mice as laboratory animals Fertility"

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Mikheeva, N. A., E. P. Drozhdina, and N. A. Kurnosova. "Morphofunctional features of proliferating cells exposed to PSMA peptide." In VIII Vserossijskaja konferencija s mezhdunarodnym uchastiem «Mediko-fiziologicheskie problemy jekologii cheloveka». Publishing center of Ulyanovsk State University, 2021. http://dx.doi.org/10.34014/mpphe.2021-142-144.

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The effect of the synthetic PSMA peptide on dividing cells of laboratory animals was studied. The experiment was carried out on male white laboratory mice of the BALB/c-line. The toxic effect of PSMA peptidi was evaluated at therapeutic (1.4 μg / kg of animal weight or 0.04 μg / animal) and subtoxic (140 μg / kg of animal weight or 4.0 μg / animal) doses. The cytotoxic effect of PSMA peptide on red bone marrow cells and cambial intestinal cells of the of laboratory mice was determined. A decrease in the proliferative activity of the colon crypt cells was revealed upon administration of a subtoxic dose of the PSMA peptide and there were no signs of toxic damage to the red bone marrow cells of animals. Key words: toxicity, proliferation, synthetic peptides, mitotic index, micronucleus test.
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Lanets, O. V., M. P. Semenenko, K. A. Semenenko, and L. V. Lazarevich. "ASSESSMENT OF THE INFLUENCE OF THE NEW STRESS CORRECTOR ON THE LABORATORY ANIMAL ORGANISM IN THE ACUTE EXPERIMENT." In STATE AND DEVELOPMENT PROSPECTS OF AGRIBUSINESS Volume 2. DSTU-Print, 2020. http://dx.doi.org/10.23947/interagro.2020.2.680-682.

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The article presents the results of acute toxicity of a new complex preparation in various ways of its introduction to laboratory white mice and rats. It was determined that a single intragastric and intramuscular administration of the maximum doses of the preparation does not cause a clinical picture of toxicosis and death of laboratory animals, on the basis of which the preparation is classified as hazardous class (GOST 12.1.007-76 “Harmful substances”) - low-hazard substances.
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Di Minno, G., A. Fusco, G. Portella, A. H. Cerbone, C. Iride, G. Tajana, O. Russo, and P. L. Mattioli. "MYELOPROLIFERATIVE DISEASE CHARACTERIZED BY THROMBOSIS, BLEEDING AND PLATELET DYSFUNCTION IN MICE INJECTED WITH THE POLYOMA MURINE LEUKEMIA VIRUS (PyMLV)." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643580.

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After i.p. injection of PyMLV, NIH/OLAC mice showed thrombi in tail veins, ears, muscles and nesenterium together with thrombi and hematomas of subcutaneous tissues. This was followed by infarctions of lungs, brain and heart, that caused death of the animals. Laboratory evaluations of the infected mice showed normochromic anemia, mild thrombocytosis and marked defects in the aggregation and in the secretion of ATP from platelets exposed to AJP, collagen, thrombin or A23137. About 10% of cells present in the bone marrow was formed by blasts; 20% by multinucleated cells identified as megakariocytes (M) by peroxidase and acethyl-cholinesterase staining, and the vast majority of the other cells by entities belonging to all stages of maturation of the myeloid lineage. Hybridization experiments showed that the blasts present in the bone marrow were the only cells in which viral replication takes place. Maturation of M in the bone marrow was completely normal, and as for M from non-infected mice, proliferation and maturation in vitro was dependent on the presence of interleukin 3. Finally, studies in other strains of mice showed that the Fv-2 locus is involved in the pathogenicity of PyMLV in NIH/OLAC mice. Ne conclude that, in addition to its obvious pathophysiological significance, the myeloproliferative disease that occurs in mice after i.p. injection of PyMLV, can serve as an important probe for understanding basic events leading to bleeding and thrombosis.
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Sudirman, Muhamad Seto. "Effectiveness of Ficus Elastica Roxb. Ex Hornem Leaf Extract in Reducing Total Cholesterol Level in High Fat Induced Diet Wistar Male Rats." In The 7th International Conference on Public Health 2020. Masters Program in Public Health, Universitas Sebelas Maret, 2020. http://dx.doi.org/10.26911/the7thicph.05.10.

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ABSTRACT Background: Kebo rubber leaves (ficus elastica roxb) contain flavonoids, polyphenols, and tannins. Flavonoids in the leaves of ficus elastica roxb such as catechins, isoflavones are polyphenolic antioxidants from plant metabolites. The leaves of ficus elastica roxb are trusted and proven empirically in the community to reduce cholesterol levels in the blood. Mice choose animals because they are considered to have physiological similarities with humans. This study aimed to determine the effect of ethanol extract of ficus elastica roxb leaves on reducing total cholesterol level in male Swiss Webster mice. Subjects and Method: This was a quasi-experimental study conducted at Biology Laboratory of the Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung from April to June, 2017. A sample of 25 male Swiss Webster mice was selected at random and allocated into groups. The dependent variable was total cholesterol. The independent variable was the extract of ficus elastica rox. The data were tested by One-Way ANOVA. Result: There were statistically significant mean differences among the study groups (p= 0.002), indicating the effect of ethanol extract of Ficus Elastica Roxb leaves on reducing total cholesterol level in male Swiss Webster mice at various doses. Conclusion: There are statistically significant mean differences among the study groups, indicating the effect of ethanol extract of Ficus Elastica Roxb leaves on reducing total cholesterol level in male Swiss Webster mice at various doses. Keyword: Ethanol extract of Ficus Elastica Roxb leaves, total cholesterol, mice Correspondence: Muhamad Seto Sudirman. School of Health Polytechnic, Pangkalpinang. Email: MuhamadSeto@gmail.com DOI: https://doi.org/10.26911/the7thicph.05.10
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Lazko, Alexey, Larisa Udochkina, and Nina Losovskaya. "Histochemical changes of the lung tissue in experimental chronic alcoholic intoxication." In Innovations in Medical Science and Education. Dela Press Publishing House, 2022. http://dx.doi.org/10.56199/dpcsms.nrjc3772.

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Among organ systems in the human body affected by alcohol abuse, the lungs are particularly vulnerable to infections and injury. Chronic alcoholism causesalterations in host defence of the upper and lower airways, disruption of alveolar epithelial barrier integrity, alcohol-induced ciliary lesions and alveolar macrophages dysfunction. Currently with a spread of SARS-COV 2 infections which instantly destroys the lung tissue, the alcohol-induced lung damage issues acquire vital importance, as they might further increase severity of lesions of lung tissue in the infected alcohol abusers.Recent investigations suggest that the effect of the chronic excessive alcohol consumption and SARS-COV 2 infection on the lungs might have similar and thus synergizing mechanisms. Therefore the mechanism of the lung tissue lesions in chronic alcohol intoxication need to be scrutinized, including the time-line of their development, to be able to develop more effective preventive measures. The objective of the study is to assess histochemical changes in the lung tissue of laboratory animals with chronic alcohol intoxication of different duration. Total of 48 outbred male white mice weighing 18-22 g were enrolled in the study. The experimental animals were exposed to alcohol for 1, 2 and 3 months by the semi-voluntary intake, using 20% alcohol as the only source of fluid, while control animals were getting drinking water. At the end of experiment the lung tissue of the mice was processed histologically and histochemically for alcoholic dehydrogenase (ADH), glucose-6-phasphate-dehydrogenae (G6PDH), alkaline (ALP) and acidic (AP) phosphatases, nonspecific esterase (NE) and succinate dehydrogenase (SDH). Image analysis of the histological slides was performed using Image Pro Plus software. Statistical differences were assessed using paired t-test. Chronic alcohol consumption causes metabolic lesions in the alveolar epithelium and endothelium of alveolar capillaries revealed by an increase in the activity of ADH, G6PD and NE paralleled with a decrease in the total SDH activity of the respiratory portion of the lungs in a time-related pattern. High activity of alkaline phosphatase was noted in endothelial cells of lung capillaries. Thus, under conditions of chronic intoxication, ethanol disturbs cell metabolism, as evidenced by the changes of the enzymatic activity in the lung tissue which leads to inhibition of oxygen-dependent metabolic processes and activation of reserve mechanisms for compensating of energy deficits.
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Reports on the topic "Mice as laboratory animals Fertility"

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Shpigel, Nahum, Raul Barletta, Ilan Rosenshine, and Marcelo Chaffer. Identification and characterization of Mycobacterium paratuberculosis virulence genes expressed in vivo by negative selection. United States Department of Agriculture, January 2004. http://dx.doi.org/10.32747/2004.7696510.bard.

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Mycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of a severe inflammatory bowel disease (IBD) in ruminants, known as Johne’s disease or paratuberculosis. Johne’s disease is considered to be one of the most serious diseases affecting dairy cattle both in Israel and worldwide. Heavy economic losses are incurred by dairy farmers due to the severe effect of subclinical infection on milk production, fertility, lower disease resistance and early culling. Its influence in the United States alone is staggering, causing an estimated loss of $1.5 billion to the agriculture industry every year. Isolation of MAP from intestinal tissue and blood of Crohn's patients has lead to concern that it plays a potential pathogenic role in promoting human IDB including Crohn’s disease. There is great concern following the identification of the organism in animal products and shedding of the organism to the environment by subclinically infected animals. Little is known about the molecular basis for MAP virulence. The goal of the original proposed research was to identify MAP genes that are required for the critical stage of initial infection and colonization of ruminants’ intestine by MAP. We proposed to develop and use signature tag mutagenesis (STM) screen to find MAP genes that are specifically required for survival in ruminants upon experimental infection. This research projected was approved as one-year feasibility study to prove the ability of the research team to establish the animal model for mutant screening and alternative in-vitro cell systems. In Israel, neonatal goat kids were repeatedly inoculated with either one of the following organisms; MAP K-10 strain and three transposon mutants of K-10 which were produced and screened by the US PI. Six months after the commencement of inoculation we have necropsied the goats and taken multiple tissue samples from the jejunum, ileum and mesenteric lymph nodes. Both PCR and histopathology analysis indicated on efficient MAP colonization of all the inoculated animals. We have established several systems in the Israeli PI’s laboratory; these include using IS900 PCR for the identification of MAP and using HSP65-based PCR for the differentiation between MAV and MAP. We used Southern blot analysis for the differentiation among transposon mutants of K-10. In addition the Israeli PI has set up a panel of in-vitro screening systems for MAP mutants. These include assays to test adhesion, phagocytosis and survival of MAP to/within macrophages, assays that determine the rate of MAPinduced apoptosis of macrophages and MAP-induced NO production by macrophages, and assays testing the interference with T cell ã Interferon production and T cell proliferation by MAP infected macrophages (macrophage studies were done in BoMac and RAW cell lines, mouse peritoneal macrophages and bovine peripheral blood monocytes derived macrophages, respectively). All partners involved in this project feel that we are currently on track with this novel, highly challenging and ambitious research project. We have managed to establish the above described research systems that will clearly enable us to achieve the original proposed scientific objectives. We have proven ourselves as excellent collaborative groups with very high levels of complementary expertise. The Israeli groups were very fortunate to work with the US group and in a very short time period to master numerous techniques in the field of Mycobacterium research. The Israeli group has proven its ability to run this complicated animal model. This research, if continued, may elucidate new and basic aspects related to the pathogenesis MAP. In addition the work may identify new targets for vaccine and drug development. Considering the possibility that MAP might be a cause of human Crohn’s disease, better understanding of virulence mechanisms of this organism might also be of public health interest as well.
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