Journal articles: 'Metpath'

1

BALL, D. G. A., and P. Y. F. ROBIN. "METPET: Metamorphic petrology microcomputer programs." Journal of Metamorphic Geology 8, no. 3 (May 1990): 251–56. http://dx.doi.org/10.1111/j.1525-1314.1990.tb00471.x.

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Zhang, Youming, Chengjun Wu, Minrong Zhu, and Jingsheng Miao. "High Performance Near-Infrared Emitters with Methylated Triphenylamine and Thiadiazolo[3,4-g]quinoxaline-Based Fluorophores." Molecules 26, no. 21 (October 22, 2021): 6386. http://dx.doi.org/10.3390/molecules26216386.

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Three near-infrared emitters (2TPA-QBT, 2MeTPA-BT and TPA-QBT-MeTPA) were rationally designed and synthesized. Density functional theory (DFT) and time-dependent density functional theory (TDDFT) calculations showed that the introduction of mono- or di-methyl groups between the donors and acceptor could result in the spatial configuration changing greatly for 2MeTPA-QBT and TPA-QBT-MeTPA compared to their parent compound 2TPA-QBT. The emission of TPA-QBT-MeTPA had a more obvious hybridized local and charge transfer feature (HLCT) based on the influence of the steric hindrance of the methyl substituent. Attributed to their different spatial configurations and luminescence mechanisms, different emission wavelengths with photoluminescent quantum yields of 26%, 38% and 34% in toluene, as well as 24%, 27% and 31% in 4,4′-bis(N-carbazolyl)-1,1′-biphenyl (CBP) doped film, were observed for 2TPA-QBT, 2MeTPA-QBT and TPA-QBT-MeTPA, respectively. The constructed organic light-emitting devices (OLEDs) displayed electroluminescence with emission peaks at 728, 693 and 710 nm, with maximum external quantum efficiencies of 1.58%, 1.33% and 3.02% for the 2TPA-QBT, 2MeTPA-QBT and TPA-QBT-MeTPA-doped OLEDs, respectively. This work illustrated the effect of spatial configuration changes on the luminescence properties of donor-acceptor-type organic emitters.

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McVeigh, J. A., S. A. Norris, N. Cameron, and J. M. Pettifor. "Associations between physical activity and bone mass in black and white South African children at age 9 yr." Journal of Applied Physiology 97, no. 3 (September 2004): 1006–12. http://dx.doi.org/10.1152/japplphysiol.00068.2004.

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We investigated differences in physical activity (PA) levels between black and white South African 9-yr-old children and their association with bone mineral content (BMC) and density (BMD) by using dual-energy X-ray absorptiometry. PA was analyzed in terms of a metabolic (METPA; weighted metabolic score of intensity, frequency, and duration) and a mechanical (MECHPA; sum of all ground reaction forces multiplied by duration) component. There were significant ethnic differences in patterns of activity. White children expended a significantly greater energy score (METPA of 21.7 ± 2.9) than black children (METPA of 9.5 ± 0.5) ( P < 0.001). When children were divided into quartiles according to the amount and intensity of sport played, the most active white children (using METPA scores) had significantly higher whole body BMD and higher hip and spine BMC and BMD than less active children. White children in the highest MECHPA quartile also showed significantly higher whole body, hip, and spine BMC and BMD than those children in the lowest quartile. No association between exercise and bone mass of black children was found. In this population, PA has an osteogenic association with white children, but not black children, which may be explained by the lower levels of PA in the black children. Despite this, black children had significantly greater bone mass at the hip and spine (girls only) ( P < 0.001) even after adjustment for body size. The role of exercise in increasing bone mass may become increasingly critical as a protective mechanism against osteoporosis in both ethnic groups, especially because the genetic benefit exhibited by black children to higher bone mass may be weakened with time, as environmental influences become stronger.

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Pokhrel, Lok R., Thilini Silva, Brajesh Dubey, Amro M. El Badawy, Thabet M. Tolaymat, and Phillip R. Scheuerman. "Rapid screening of aquatic toxicity of several metal-based nanoparticles using the MetPLATE™ bioassay." Science of The Total Environment 426 (June 2012): 414–22. http://dx.doi.org/10.1016/j.scitotenv.2012.03.049.

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Bitton, Gabriel, Ben Koopman, and Orna Agami. "MetPAD™: a bioassay for rapid assessment of heavy metal toxicity in wastewater." Water Environment Research 64, no. 6 (September 1992): 834–36. http://dx.doi.org/10.2175/wer.64.6.12.

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Xia, J., and D. S. Wishart. "MetPA: a web-based metabolomics tool for pathway analysis and visualization." Bioinformatics 26, no. 18 (July 13, 2010): 2342–44. http://dx.doi.org/10.1093/bioinformatics/btq418.

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Zhang, Yunwu, Wenxue Chen, Haiming Chen, Qiuping Zhong, Yonghuan Yun, and Weijun Chen. "Metabolomics Analysis of the Deterioration Mechanism and Storage Time Limit of Tender Coconut Water during Storage." Foods 9, no. 1 (January 3, 2020): 46. http://dx.doi.org/10.3390/foods9010046.

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Tender coconut water tastes sweet and is enjoyed by consumers, but its commercial development is restricted by an extremely short shelf life, which cannot be explained by existing research. UPLC-MS/MS-based metabolomics methods were used to identify and statistically analyze metabolites in coconut water under refrigerated storage. A multivariate statistical analysis method was used to analyze the UPLC-MS/MS datasets from 35 tender coconut water samples stored for 0–6 weeks. In addition, we identified other differentially expressed metabolites by selecting p-values and fold changes. Hierarchical cluster analysis and association analysis were performed with the differentially expressed metabolites. Metabolic pathways were analyzed using the KEGG database and the MetPA module of MetaboAnalyst. A total of 72 differentially expressed metabolites were identified in all groups. The OPLS-DA score chart showed that all samples were well grouped. Thirty-one metabolic pathways were enriched in the week 0–1 samples. The results showed that after a tender coconut is peeled, the maximum storage time at 4 °C is 1 week. Analysis of metabolic pathways related to coconut water storage using the KEGG and MetPA databases revealed that amino acid metabolism is one of the main causes of coconut water quality deterioration.

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Brohon, B., and R. Gourdon. "Influence of soil microbial activity level on the determination of contaminated soil toxicity using Lumistox and MetPlate bioassays." Soil Biology and Biochemistry 32, no. 6 (June 2000): 853–57. http://dx.doi.org/10.1016/s0038-0717(99)00216-3.

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Forward, Jennifer M., John P. Fackler, and Richard J. Staples. "Synthesis and Structural Characterization of the Luminescent Gold(I) Complex [(MeTPA)3AuI]I3. Use of NaBPh4 as a Phenyl-Transfer Reagent To Form [(MeTPA)AuPh](BPh4) and (TPA)AuPh." Organometallics 14, no. 9 (September 1995): 4194–98. http://dx.doi.org/10.1021/om00009a022.

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Boularbah, Ali, Gabriel Bitton, Jean Louis Morel, and Christophe Schwartz. "Assessment of metal accumulation in plants using MetPAD, a toxicity test specific for heavy metal toxicity." Environmental Toxicology 15, no. 5 (2000): 449–55. http://dx.doi.org/10.1002/1522-7278(2000)15:5<449::aid-tox13>3.0.co;2-c.

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Alwahsh, Mohammad, Robert Knitsch, Rosemarie Marchan, Jörg Lambert, Christian Hoerner, Xiaonan Zhang, Berthold Schalke, et al. "Metabolic Profiling of Thymic Epithelial Tumors Hints to a Strong Warburg Effect, Glutaminolysis and Precarious Redox Homeostasis as Potential Therapeutic Targets." Cancers 14, no. 6 (March 18, 2022): 1564. http://dx.doi.org/10.3390/cancers14061564.

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Thymomas and thymic carcinomas (TC) are malignant thymic epithelial tumors (TETs) with poor outcome, if non-resectable. Metabolic signatures of TETs have not yet been studied and may offer new therapeutic options. Metabolic profiles of snap-frozen thymomas (WHO types A, AB, B1, B2, B3, n = 12) and TCs (n = 3) were determined by high resolution magic angle spinning 1H nuclear magnetic resonance (HRMAS 1H-NMR) spectroscopy. Metabolite-based prediction of active KEGG metabolic pathways was achieved with MetPA. In relation to metabolite-based metabolic pathways, gene expression signatures of TETs (n = 115) were investigated in the public “The Cancer Genome Atlas” (TCGA) dataset using gene set enrichment analysis. Overall, thirty-seven metabolites were quantified in TETs, including acetylcholine that was not previously detected in other non-endocrine cancers. Metabolite-based cluster analysis distinguished clinically indolent (A, AB, B1) and aggressive TETs (B2, B3, TCs). Using MetPA, six KEGG metabolic pathways were predicted to be activated, including proline/arginine, glycolysis and glutathione pathways. The activated pathways as predicted by metabolite-profiling were generally enriched transcriptionally in the independent TCGA dataset. Shared high lactic acid and glutamine levels, together with associated gene expression signatures suggested a strong “Warburg effect”, glutaminolysis and redox homeostasis as potential vulnerabilities that need validation in a large, independent cohort of aggressive TETs. If confirmed, targeting metabolic pathways may eventually prove as adjunct therapeutic options in TETs, since the metabolic features identified here are known to confer resistance to cisplatin-based chemotherapy, kinase inhibitors and immune checkpoint blockers, i.e., currently used therapies for non-resectable TETs.

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Shao, Changzhuan, Jing Song, Shanguang Zhao, Hongke Jiang, Baoping Wang, and Aiping Chi. "Therapeutic Effect and Metabolic Mechanism of A Selenium-Polysaccharide from Ziyang Green Tea on Chronic Fatigue Syndrome." Polymers 10, no. 11 (November 15, 2018): 1269. http://dx.doi.org/10.3390/polym10111269.

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Ziyang green tea was considered a medicine food homology plant to improve chronic fatigue Ssyndrome (CFS) in China. The aim of this research was to study the therapeutic effect of selenium-polysaccharides (Se-TP) from Ziyang green tea on CFS and explore its metabolic mechanism. A CFS-rats model was established in the present research and Se-TP was administrated to evaluate the therapeutic effect on CFS. Some serum metabolites including blood urea nitrogen (BUN), blood lactate acid (BLA), corticosterone (CORT), and aldosterone (ALD) were checked. Urine metabolites were analyzed via gas chromatography-mass spectrometry (GC-MS). Multivariate statistical analysis was also used to check the data. The results selected biomarkers that were entered into the MetPA database to analyze their corresponding metabolic pathways. The results demonstrated that Se-TP markedly improved the level of BUN and CORT in CFS rats. A total of eight differential metabolites were detected in GC-MS analysis, which were benzoic acid, itaconic acid, glutaric acid, 4-acetamidobutyric acid, creatine, 2-hydroxy-3-isopropylbutanedioic acid, l-dopa, and 21-hydroxypregnenolone. These differential metabolites were entered into the MetPA database to search for the corresponding metabolic pathways and three related metabolic pathways were screened out. The first pathway was steroid hormone biosynthesis. The second was tyrosine metabolism, and the third was arginine-proline metabolism. The 21-hydroxypregnenolone level of rats in the CFS group markedly increased after the Se-TP administration. In conclusion, Se-TP treatments on CFS rats improved their condition. Its metabolic mechanism was closely related to that which regulates the steroid hormone biosynthesis.

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Bitton, Gabriel, Marjorie Campbell, and Ben Koopman. "MetPAD: A bioassay kit for the specific determination of heavy metal toxicity in sediments from hazardous waste sites." Environmental Toxicology & Water Quality 7, no. 4 (November 1992): 323–28. http://dx.doi.org/10.1002/tox.2530070403.

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Sumiyati, I. Wayan Windia, and I. Wayan Tika. "Operasional dan pemeliharaan jaringan irigasi subak di Kabupaten Tabanan." Jurnal Kajian Bali (Journal of Bali Studies) 7, no. 1 (May 18, 2017): 121. http://dx.doi.org/10.24843/jkb.2017.v07.i01.p08.

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This research was conducted in order to understand the operational and maintenance of irrigation network in traditional Balinese irrigation system of subak in Tabanan regency. It spesific aim is to develop a documentation which is useful as reference about operational and maintenance of irrigation network in subak system. The sample of this research are four subak in Tabanan regency which are subak in the upstream, middle, and downstream areas, including Subak Bunyuh, Subak Sigaran, Subak Guama, and Subak Sungsang. The research was conducted by survey and depth interview methods. The operational and maintenance of irrigation networks in subak system in Tabanan regency includes matelik (monitoring channel), nyilih yeh (borrow the water), magilihan (rotation of water irigation), kempelan (maintenance of traditional weir), ngeduk nyanyad (draining mud), nabdab yeh (arrangement of water irrigation distribution), metpet munduk (lining stabilitation.), and ngampad (chanel clearing). This article concludes that all subak samples in Tabanan regency carry out operational and maintenance activities the irrigation networks in the subak system.

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Picanço, Giorgio Arlan da Silva, Julio Tota da Silva, Raoni Aquino Silva de Santana, and Roseilson Souza do Vale. "DESENVOLVIMENTO DE UMA SONDA ATMOSFÉRICA SEM FIO PARA ESTUDOS AMBIENTAIS DE BAIXO CUSTO." Ciência e Natura 38 (July 20, 2016): 118. http://dx.doi.org/10.5902/2179460x20112.

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This paper presents the development of a wireless atmospheric probe, designed to assist long-term environmental studies with limited financial resources. The work is based on the concept of Wireless Sensor Networks in which multiple sensors are arranged spatially in a particular region. Facilitating the monitoring of large areas without the need for large physical structures of communication and power, as cables and towers. The prototype, built with cheap material, captures temperature data, pressure and humidity, transmitting this information in real time to a remote receiver via radio frequency to a distance of 1 km. The prototype is also equipped with a photovoltaic system capable of guaranteeing energy independence, an essential factor for long-term studies. To evaluate the efficiency of the prototype, correlation tests were performed with a Metpak station II (Gill Instruments), to simulate the continuous operation of the sensors in situ. The tests show significant correlations between atmospheric pressure variables (98%), relative humidity (87%) and air temperature (97%). The tests ensure a good degree of reliability to the prototype; can serve as an alternative to environmental monitoring projects and Wireless Sensor Networks.

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Feng, Nan, Hossein Ghoveisi, Gabriel Bitton, and Jean-Claude J. Bonzongo. "Removal of phyto-accessible copper from contaminated soils using zero valent iron amendment and magnetic separation methods: Assessment of residual toxicity using plant and MetPLATE™ studies." Environmental Pollution 219 (December 2016): 9–18. http://dx.doi.org/10.1016/j.envpol.2016.09.050.

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Gao, Xiaoyan, Mingxing Guo, Long Peng, Baosheng Zhao, Jiankun Su, Haiyu Liu, Li Zhang, Xu Bai, and Yanjiang Qiao. "UPLC Q-TOF/MS-Based Metabolic Profiling of Urine Reveals the Novel Antipyretic Mechanisms of Qingkailing Injection in a Rat Model of Yeast-Induced Pyrexia." Evidence-Based Complementary and Alternative Medicine 2013 (2013): 1–8. http://dx.doi.org/10.1155/2013/864747.

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Fever is one of the most common clinical symptoms of many diseases. Qingkailing (QKL) injection is widely used in China as a clinical emergency medicine due to its good antipyretic effects. It is a herbal formula which is composed by eight kinds of traditional Chinese medicines (TCM). As a kind of typical multiple constituents and multiple actions of TCM, it is very difficult to elaborate the antipyretic mechanism by conventional pharmacological method. Metabonomics technique provides beneficial tool for this challenge. In this study, an ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC Q-TOF/MS) metabonomics method was developed to explore the changing process of biochemical substances in rats of yeast-induced pyrexia. Partial least squares discriminate analysis (PLS-DA) was used to distinguish the normal control group, the pyrexia model group, and the pyrexia model group treated by QKL injection. The potential biomarkers related to pyrexia were confirmed and identified. MetPA was used to find the possible metabolic pathways. The results indicated that the antipyretic effect of QKL injection on yeast-induced pyrexia rats was performed by repairing the perturbed metabolism of amino acids.

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Su, Yuanyuan, Weixing Yang, Xu Yang, Ronglan Zhang, and Jianshe Zhao. "Visible Light-Induced CO-Release Reactivity of a Series of ZnII–Flavonolate Complexes." Australian Journal of Chemistry 71, no. 8 (2018): 549. http://dx.doi.org/10.1071/ch18192.

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A series of zinc–flavonolate complexes of the general formula [(L)Zn(R)]ClO4 (L = TPA (tris-2-(pyridylmethyl)amine)), 6-MeTPA (N,N-(6-methyl-2-pyridyl)methyl)bis(2-pyridylmethyl)amine)), 6-Me2TPA (N,N-bis(6-methyl-2-pyridyl)methyl)(2-pyridylmethyl) amine), BPQA (bis(2-pyridylmethyl)(2-quinolinemethyl)amine), and BQPA (bis(2-quinolinemethyl)(2-pyridylmethyl)amine), R = FLH (flavonol), 4-MeOFLH (4-methoxyflavonol), and 4-MeOFLTH (4-methoxyflavothione)) have been prepared and characterised by X-ray crystallography, elemental analysis, FT-IR, ESI-MS, 1H NMR, 13C NMR, UV-vis and fluorescence spectroscopy. All the complexes can be induced to release CO by visible light (λmax ranges from 414 to 503 nm). The maximum absorption wavelength of the complexes followed the order 4-MeOFLTH > 4-MeOFLH > FLH. Exposure of the complexes to visible light under aerobic conditions results in oxidative C–C bond cleavage and almost quantitative CO release. Cytotoxicity tests showed that the complexes had a low toxicity to HeLa cells in the concentration range of 1 to 50 μM. These advantages indicate that the series of complexes are likely to be applied to biological systems.

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Zhang, Bo, Jie Zhou, Qiang Li, Bingcheng Gan, Weihong Peng, Xiaoping Zhang, Wei Tan, Lin Jiang, and Xiaolin Li. "Manganese affects the growth and metabolism ofGanoderma lucidumbased on LC-MS analysis." PeerJ 7 (May 1, 2019): e6846. http://dx.doi.org/10.7717/peerj.6846.

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BackgroundAs a metal-enriched edible fungus,Ganoderma lucidumis capable of adsorbing manganese effectively. And the manganese ion is demonstrated to play an important role in the synthesis of manganese peroxidase (Mnp) and other physiological activities duringG. lucidumgrowth. Recently, the influence of manganese on the metabolites ofG. lucidumfruiting bodies can be revealed through metabonomics technique.MethodsIn this study, we uncovered the changes between the control and 200 mg/kg Mn-treated fruiting bodies with liquid chromatography coupled to mass spectrometry (LC-MS).ResultsThe mycelial growth rate, dry yield, Mnp activity , total polysaccharide content, triterpenoid content, and total manganese content in the mature fruiting bodies ofG. lucidumchanged between the control and different Mn-treated groups. Based on LC-MS method, a total of 16 significantly different metabolites were obtained and identified, among which, five presented significantly down-regulated and 11 up-regulated in Mn-treated samples. The metabolites chavicol and palmitoylethanolamide were particularly significantly up-regulated, and were found the strong promotion relationship. Dependent on the MetPA database, four KEGG pathways were detected and glycerophospholipid metabolism was most impacted, in which, choline was involved in.DiscussionThe added manganese ion in the substrate enhanced Mnp activities, and consequently promoted the mycelial growth, yield , metabolites in the fruiting bodies including triterpenoids, total manganese, chavicol, etc. Our finding can provide a theoretical reference to regulation of manganese on the physiological metabolism ofG. lucidum.

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Qabur, Ali, Hassan Baaj, and Mohab El-Hakim. "Incorporation of the Multi-Layer Plastic Packaging in the Asphalt Binders: Physical, Thermal, Rheological, and Storage Properties Evaluation." Polymers 14, no. 24 (December 9, 2022): 5396. http://dx.doi.org/10.3390/polym14245396.

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The amount of residual Multi-layer Plastic Packaging (MPP) in Canada has greatly increased in the last two decades, which has economic and environmental consequences. MPP is primarily made up of two or more layers of Polyethylene (PE), Polyester (PET), Nylon (NY), and Metalized Polyester (METPET). While MPP has not been used as an asphalt modifier, some of the materials commonly found in MPP, such as PE and PET, have also been successfully used as asphalt modifiers. Nevertheless, a few recent studies have demonstrated the potential for reusing MPP as an asphalt modifier to improve asphalt pavement performance. Recycling post-industrial MPP instead of using raw polymers could lead to economic and environmental benefits. However, a comprehensive study to evaluate MPP as a viable asphalt additive is lacking. The main objective of this study is to evaluate the feasibility of using MPP as an asphalt modifier via the wet method, considering the physical, thermal, rheological, and storage properties of the MPP-modified binder at different MPP concentrations (2%, 4%, and 8%) in asphalt cement (PG 58–28). MPP-modified binders were evaluated using the following instruments: Differential Scanning Calorimeter (DSC), Thermogravimetric Analysis (TGA), Superpave Dynamic Shear Rheometer (DSR), Rotational Viscosity (RV), and Environmental Scanning Electron Microscopy (ESEM). Test results indicated that the incorporation of MPP has a strong potential to improve permanent deformation resistance at high temperatures. In addition, MPP shows a moderate impact on fatigue cracking performance at intermediate temperatures. Overall, in low-temperature climates, using less than 4% of MPP additives would offer higher fatigue damage resistance along with adequate permanent deformation. In high-temperature climates, higher concentrations of additives may be preferable to resist permanent deformation. Finally, MPP is a challenge for existing recycling systems, and its incorporation into asphalt applications may develop more sustainable materials that would contribute to circular economy principles.

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Jin, Hua, Ningning Ma, Xin Li, Mingqin Kang, Maojuan Guo, and Lili Song. "Application of GC/MS-Based Metabonomic Profiling in Studying the Therapeutic Effects of Aconitum carmichaeli with Ampelopsis japonica Extract on Collagen-Induced Arthritis in Rats." Molecules 24, no. 10 (May 20, 2019): 1934. http://dx.doi.org/10.3390/molecules24101934.

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Aconitum carmichaeli with Ampelopsis japonica (AA) is a classical traditional Chinese medicine (TCM) formula. There are a lot of examples showing that AA can be used to treat rheumatoid arthritis, but its mechanism of action is still not completely clear. In this research, collagen-induced arthritis (CIA) was chosen as a rheumatoid arthritis (RA) model. Rats of treated groups were continuously administered Aconitum carmichaeli (AC), Ampelopsis japonica (AJ) and Aconitum carmichaeli + Ampelopsis japonica (AA) orally once a day from the day after the onset of arthritis (day 7) until day 42. The results showed that AA not only significantly reduced paw swelling, but also improved the levels of TNF-α and IL-6 in serum. GC-MS-based urine metabonomics was established to analysis metabolic profiles and 21 biomarkers of RA rats were identified by the Partial Least Squares Discriminant Analysis (PLS-DA) and Support Vector Machine (SVM) methods. The prediction rate of the SVM method for the 21 biomarkers was 100%. Twenty of 21 biomarkers, including D-galactose, inositol and glycerol, gradually returned to normal levels after administration of AA. Metabolomic Pathway Analysis (MetPA) generated three related metabolic pathways—galactose metabolism, glycerolipid metabolism and inositol phosphate metabolism—which explain the mechanism of AA treatment of rheumatoid arthritis. This research provides a better understanding of the therapeutic effects and possible therapeutic mechanism of action of a complex TCM (AA) on rheumatoid arthritis.

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HOUBEN, Roger J. T. J., Dirk T. S. RIJKERS, Thomas B. STANLEY, Francine ACHER, Robert AZERAD, Sanna-Maria KÄKÖNEN, Cees VERMEER, and Berry A. M. SOUTE. "Characteristics and composition of the vitamin K-dependent γ-glutamyl carboxylase-binding domain on osteocalcin." Biochemical Journal 364, no. 1 (May 8, 2002): 323–28. http://dx.doi.org/10.1042/bj3640323.

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Two different sites on vitamin K-dependent γ-glutamyl carboxylase (VKC) are involved in enzyme—substrate interaction: the propeptide-binding site required for high-affinity substrate binding and the active site for glutamate carboxylation. Synthetic descarboxy osteocalcin (d-OC) is a low-Km substrate for the VKC, but unique since it possesses a high-affinity recognition site for the VKC, distinct from the propeptide which is essential as a binding site for VKC. However, the exact location and composition of this VKC-recognition domain on d-OC has remained unclear until now. Using a stereospecific substrate analogue [t-butyloxycarbonyl-(2S,4S)-4-methylglutamic acid-Glu-Val (S-MeTPT)] we demonstrate in this paper that the high affinity of d-OC for VKC cannot be explained by a direct interaction with either the active site or with the propeptide-binding site on VKC. It is shown using various synthetic peptides derived from d-OC that there are two domains on d-OC necessary for recognition: one located between residues 1 and 12 and a second between residues 26 and 39, i.e. at the C-terminal side of the γ-carboxyglutamate (Gla) domain. Both internal sequences contribute substantially to the efficiency of carboxylation. On the basis of these data we postulate the presence of a second high-affinity substrate-binding site on VKC capable of specifically binding d-OC, which is the first vitamin K-dependent substrate of which the VKC binding domain is interrupted by the Gla domain.

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Liu, Yuetao, Jiajia Cui, Yinghuan Hu, Guanhua Du, and Xuemei Qin. "Integrating untargeted metabonomics, partial least square regression analysis and MetPA to explore the targeted pathways involved into Huangqi Jiangzhong Tang against chronic atrophic gastritis rats." Chemometrics and Intelligent Laboratory Systems 164 (May 2017): 16–25. http://dx.doi.org/10.1016/j.chemolab.2017.03.005.

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Zhao, Shanguang, Aiping Chi, Junhu Yan, and Chong Yao. "Feature of Heart Rate Variability and Metabolic Mechanism in Female College Students with Depression." BioMed Research International 2020 (February 28, 2020): 1–9. http://dx.doi.org/10.1155/2020/5246350.

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Purpose. To explore the effects of depression on cardiac autonomic nerve function and related metabolic pathways, the heart rate variability (HRV) and urinary differential metabolites were detected on the college students with depression. Methods. 12 female freshmen with depression were filtered by the Beck Depression Inventory (BDI-II) and Self-rating Depression Scale (SDS). By wearing an HRV monitoring system, time domain indexes and frequency domain indexes were measured over 24 hours. Liquid chromatography–mass spectrometry (LC-MS) was used to detect their urinary differential metabolites. Differential metabolites were identified by principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA). The metabolic pathways related to these differential metabolites were analyzed by the MetPA database. Results. Stress time was significantly increased, and recovery time was markedly decreased in the depression group compared with the control group (p<0.001). Standard deviation of the normal-to-normal R interval (SDNN), root mean square of the beat-to-beat differences (RMSSD), high frequency (HF), and low frequency (LF) were decreased significantly (p<0.01). Moreover, 15 differential metabolites (4↑, 11↓) were identified in the depression group. These differential metabolites were involved in the disruption of five metabolic pathways (coenzyme Q biosynthesis, glycine-serine-threonine metabolism, tyrosine metabolism, pyrimidine metabolism, and steroid metabolism). Conclusion. Some autonomic nervous system disruption, high stress, and poor fatigue recovery were confirmed in college students with depression. The metabolic mechanism involved the disruption of coenzyme Q biosynthesis, glycine-serine-threonine metabolism, tyrosine metabolism, pyrimidine metabolism, and steroid metabolism under daily stress.

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Cao, Ben, Shuojia Liu, Lin Yang, and Aiping Chi. "Changes of Differential Urinary Metabolites after High-Intensive Training in Teenage Football Players." BioMed Research International 2020 (March 20, 2020): 1–9. http://dx.doi.org/10.1155/2020/2073803.

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Objective. The mechanism underlying the fatigue of football players is closely related to the energy depletion and accumulation of metabolites; the present study tries to explore the metabolic mechanism in teenage football players during exercise-induced fatigue. Methods. 12 teenage football players were subjected to three groups of combined training by using a cycle ergometer, with the subjective Rating of Perceived Exertion (RPE) as a fatigue criterion. The following indicators were measured in each group after training: maximum oxygen uptake (VO2max), anaerobic power, and average anaerobic power. Urine samples were collected before and after the training. Gas chromatography-mass spectrometry (GC-MS) was performed for the metabonomics analysis of the samples. The metabolism data was analyzed by using principal component analysis (PCA) and orthogonal partial least squares analysis (OPLS-DA), through the Kyoto Encyclopedia of Genes and Genomes (KEGG) database to confirm the potential differences between metabolites, and the MetPA database was used to analyze the related metabolic pathways. Results. There was no significant difference between the maximal oxygen uptakes among the three groups. Compared with group 1, the maximum and average anaerobic power in group 3 significantly decreased (p<0.05) at the end of training. GC-MS detected 635 metabolites in the urine samples. Through PCA, OPLS-DA analysis, and KEGG matching, 25 different metabolites (3↑22↓) that met the conditions were finally selected. These different metabolites belonged to 5 metabolic pathways: glycine-serine-threonine metabolism, citrate cycle, tyrosine metabolism, nitrogen metabolism, and glycerophospholipid metabolism. Conclusions. During the combined exercise of aerobic and anaerobic metabolism, teenage football players show a significant decrease in anaerobic capacity after fatigue. The metabolic mechanism of exercise fatigue was related to disorders in amino acid and energy metabolism.

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Offor, Benedict C., Msizi I. Mhlongo, Paul A. Steenkamp, Ian A. Dubery, and Lizelle A. Piater. "Untargeted Metabolomics Profiling of Arabidopsis WT, lbr-2-2 and bak1-4 Mutants Following Treatment with Two LPS Chemotypes." Metabolites 12, no. 5 (April 22, 2022): 379. http://dx.doi.org/10.3390/metabo12050379.

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Plants perceive pathogenic threats from the environment that have evaded preformed barriers through pattern recognition receptors (PRRs) that recognise microbe-associated molecular patterns (MAMPs). The perception of and triggered defence to lipopolysaccharides (LPSs) as a MAMP is well-studied in mammals, but little is known in plants, including the PRR(s). Understanding LPS-induced secondary metabolites and perturbed metabolic pathways in Arabidopsis will be key to generating disease-resistant plants and improving global plant crop yield. Recently, Arabidopsis LPS-binding protein (LBP) and bactericidal/permeability-increasing protein (BPI)-related proteins (LBP/BPI related-1) and (LBP/BPI related-2) were shown to perceive LPS from Pseudomonas aeruginosa and trigger defence responses. In turn, brassinosteroid insensitive 1 (BRI1)-associated receptor kinase 1 (BAK1) is a well-established co-receptor for several defence-related PRRs in plants. Due to the lack of knowledge pertaining to LPS perception in plants and given the involvement of the afore-mentioned proteins in MAMPs recognition, in this study, Arabidopsis wild type (WT) and mutant (lbr2-2 and bak1-4) plants were pressure-infiltrated with LPSs purified from Pseudomonas syringae pv. tomato DC3000 (Pst) and Xanthomonas campestris pv. campestris 8004 (Xcc). Metabolites were extracted from the leaves at four time points over a 24 h period and analysed by UHPLC-MS, generating distinct metabolite profiles. Data analysed using unsupervised and supervised multivariate data analysis (MVDA) tools generated results that reflected time- and treatment-related variations after both LPS chemotypes treatments. Forty-five significant metabolites were putatively annotated and belong to the following groups: glucosinolates, hydroxycinnamic acid derivatives, flavonoids, lignans, lipids, oxylipins, arabidopsides and phytohormones, while metabolic pathway analysis (MetPA) showed enrichment of flavone and flavanol biosynthesis, phenylpropanoid biosynthesis, alpha-linolenic acid metabolism and glucosinolate biosynthesis. Distinct metabolite accumulations depended on the LPS chemotype and the genetic background of the lbr2-2 and bak1-4 mutants. This study highlights the role of LPSs in the reprogramming Arabidopsis metabolism into a defensive state, and the possible role of LBR and BAK1 proteins in LPSs perception and thus plant defence against pathogenic bacteria.

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Hajiashrafi, Taraneh, Ali Nemati Kharat, Jennifer A. Love, and Brian O. Patrick. "Synthesis, characterization and crystal structure of three new lanthanide (III) complexes with the [(6-methyl-2-pyridyl)methyl]bis(2-pyridylmethyl)amine (MeTPA) ligand; New precursors for lanthanide (III) oxide nano-particles." Polyhedron 60 (August 2013): 30–38. http://dx.doi.org/10.1016/j.poly.2013.04.061.

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van Dijken, Bart R. J., Bram Schuuring, Hanne-Rinck Jeltema, Peter Jan van Laar, Roelien H. Enting, Rudi A. J. O. Dierckx, Gilles N. Stormezand, and Anouk van der Hoorn. "Ventricle contact may be associated with higher 11C methionine PET uptake in glioblastoma." Neuroradiology, June 10, 2021. http://dx.doi.org/10.1007/s00234-021-02742-7.

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Abstract Purpose Ventricle contact is associated with a worse prognosis and more aggressive tumor characteristics in glioblastoma (GBM). This is hypothesized to be a result of neural stem cells located around the lateral ventricles, in the subventricular zone. 11C Methionine positron emission tomography (metPET) is an indicator for increased proliferation, as it shows uptake of methionine, an amino acid needed for protein synthesis. This study is the first to study metPET characteristics of GBM in relation to ventricle contact. Methods A total of 12 patients with IDH wild-type GBM were included. Using MRI, the following regions were determined: primary tumor (defined as contrast enhancing lesion on T1) and peritumoral edema (defined as edema visible on FLAIR excluding the enhancement). PET parameters in these areas were extracted using PET fused with MRI imaging. Parameters extracted from the PET included maximum and mean tumor-to-normal ratio (TNRmax and TNRmean) and metabolic tumor volume (MTV). Results TNRmean of the primary tumor showed significantly higher values for the ventricle-contacting group compared to that for the non-contacting group (4.44 vs 2.67, p = 0.030). Other metPET parameters suggested higher values for the ventricle-contacting group, but these differences did not reach statistical significance. Conclusion GBM with ventricle contact demonstrated a higher methionine uptake and might thus have increased proliferation compared with GBM without ventricle contact. This might explain survival differences and should be considered in treatment decisions.

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Martínez-Villalba, José Antonio, Mayra Yudirian Luna-Porres, Alejandra Mayela Gilio-Villa, and Sebastián Jafet Arredondo-Guerrero. "Microbiological and total particles suspended indoor air quality at the Universidad Iberoamericana Torreón." Journal of Enviromental Sciences and Natural Resources, December 31, 2020, 20–34. http://dx.doi.org/10.35429/jesn.2020.18.6.20.34.

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Indoor air quality was analyzed in the period from 2016 to 2019, in the Universidad Iberoamericana Torreón, with the objective of determining if it complied with the reference norms: ISO 14644- 1-2015 and UNE 100012:2005; due to in Mexico there are no applicable norms for the monitoring or indication of the Maximum Permissible Limits to determine indoor air quality. Methodology applied was focused on: 1) Determining the PSTs (from 0.3 to 10 µm) using a FLUKE particle counter; 2) Microbiological analysis; 3) Identifying isolated strains and 4) Calculating origin and wind intensity using a METPAK II climatological data and a WRPLOT software. PST and microbiological concentrations were found to be above the standards used. PS0.3 showed a significant difference (p<0.05) in the Auditorium, which had the highest concentrations in 2019 of evening schedule. Some of the biological material were identified as: 1) pathogenic bacteria of the genera Salmonella, Klebisella and Escherichia, and 2) fungi of the genera Aspergillus, Trichophyton and Candida; whose exposure could produce different allergies, asthma, develop respiratory symptoms, chronic cough, laryngitis, sinusitis and urticaria.

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Yuan, Yin, Fei-Xue Dong, Xu Liu, Hong-Bin Xiao, and Zhong-Guang Zhou. "Liquid Chromatograph-Mass Spectrometry-Based Non-targeted Metabolomics Discovery of Potential Endogenous Biomarkers Associated With Prostatitis Rats to Reveal the Effects of Magnoflorine." Frontiers in Pharmacology 12 (November 1, 2021). http://dx.doi.org/10.3389/fphar.2021.741378.

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Magnoflorine (Mag) has multiple pharmacological activities for the prevention and treatment of prostatitis. However, its molecular mechanisms andpharmacological targets are not clear. In this study, the ultra-performance liquid tandem mass spectrometry-based metabolomics method was used to clarify the intervention of Mag against prostatitis and the biological mechanism. A total of 25 biomarkers associated with the prostatitis model were identified by metabolomics, and a number of metabolic pathways closely related to the model were obtained by MetPA analysis. After given Mag treatment, the results of each indicator were shown that Mag alkaloid could inhibit the development of prostatitis effectively. We found that Mag had regulative effects on potential biomarkers of prostatitis model, which can regulate them to the control group. Our results indicated that alkaloids have an effective intervention therapy for prostatitis, and five types of metabolic pathways closely related to prostatitis model were obtained, including phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, tyrosine metabolism, arginine and proline metabolism, glycine, serine and threonine metabolism, alanine, aspartate and glutamate metabolism. This study has provided the basic experimental data for the development of Mag in the prevention and treatment of prostatitis.

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Journal articles on the topic 'Metpath'

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