Journal articles: 'Method 141'

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Извекова, T. Izvekova, Гриневич, V. Grinevich, Гущин, A. Gushchin, Иванцова, and N. Ivantsova. "Bioassay Method in the Estimation of Overall Performance of Plazmachemical Water Treatment Devices." Safety in Technosphere 1, no. 4 (August 25, 2012): 47–53. http://dx.doi.org/10.12737/141.

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This article describes the estimation of wastewater treatment plasmachemical devices using toxicologic testing of model aqueous solutions of organic compounds. It is shown that the bioassay can be used not only as an effective method for assessing the toxicity of water, but also as a criterion for determination of the effectiveness of various methods of water treatment.

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Beets-Tan, R. "141 Rectal cancer staging: which method is optimal?" European Journal of Cancer Supplements 7, no. 2 (September 2009): 35. http://dx.doi.org/10.1016/s1359-6349(09)70124-7.

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KOYAMA, Akinori, Mariko HONDA, and Haruki OBARA. "141 Treatment of rigid wall in SPH method." Proceedings of The Computational Mechanics Conference 2006.19 (2006): 339–40. http://dx.doi.org/10.1299/jsmecmd.2006.19.339.

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Smith, M. A., G. Palmiotti, E. E. Lewis, and N. Tsoulfanidis. "An Integral Form of the Variational Nodal Method." Nuclear Science and Engineering 146, no. 2 (February 2004): 141–51. http://dx.doi.org/10.13182/nse146-141.

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Rada, T., R. L. Reis, and M. E. Gomes. "Novel method for the isolation of adipose stem cells (ASCs)." Journal of Tissue Engineering and Regenerative Medicine 3, no. 2 (February 2009): 158–59. http://dx.doi.org/10.1002/term.141.

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Hamdi, Saibatul, and Arhamsyah Arhamsyah. "PENGGUNAAN BAHAN FINISHING PADA ROTAN NON KOMERSIAL SEBAGAI BAHAN KERAJINAN." Jurnal Riset Industri Hasil Hutan 3, no. 2 (December 31, 2011): 7. http://dx.doi.org/10.24111/jrihh.v3i2.1189.

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Development of the rattan furniture industry today is quite fast although the amount of raw materials are decreasing, especially in commersial rattan commodities such as Manau, Irit, Taman, Sega, etc. Their price is quite expensive too five species of rattan were used as research materials. There are Manau (Calamus mettanensis Becc), Toho (Calamus sp), Galang (Daemonorops verticilaris Griff Mart), Hijau (Calamus sp) and simpurut (Calamus panajuga Becc). For finishing materials, clear glass melamine (ML 131 and NC 141) were used in this research with three different immersion timer for 24 hours, 36 hours and 48 hours long. An immersion method without using any finishing materials was done as control materials. Result show that the average of volume expansion of rattan speseis using NC 141 are 1,56-3,16% in Marau; 1,36-2,91%; in Toho; 2,01-3,05% in Galang; 2,12-3,20% in Hijau and 1,90-2,14 in Simpurut. In the other hand, the average of volume expansion of rattan species using melamin ML 131 are 1,27-2,58% in Marau; 1,46-2,51% in Toho; 2,30-3,23% in galang; 1,10-2,79% in Hijau and 1,45-2,55% in Simpurut. In control materials the average of expantion volume in 5 species rattans is 2,53-3,99%. From those results, the best method was shown in using NC 141 which can prevent the volume expansion and give great influence in volume expansion value.Keywords: rattan, finishing material, melamine ML 131, melamine NC 141, volume expantion.

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Coyle, David A. "A method of sampling clast-rich laminated diamictons for magnetic analyses." Canadian Journal of Earth Sciences 26, no. 8 (August 1, 1989): 1654–56. http://dx.doi.org/10.1139/e89-141.

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The sampling of nonlithified diamictons for magnetic analyses (anisotropy of magnetic susceptibility and paleomagnetic remanence) has been restricted to samples that are either massive, clay-rich sediments, or fine-grained ones with very low pebble contents. A new method was developed that produced standard-sized 2 × 2 × 2 cm cubes of sediment from a laminated, clay-poor diamicton from southern Denmark. The sampling procedure did not affect the sample or its magnetic characteristics in any observable manner.

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Sierra-Arguello, Yuli M., Rafaela B. Morgan, Gustavo Perdoncini, Leonardo M. Lima, Marcos José P. Gomes, and Vladimir Pinheiro do Nascimento. "Resistance to β-lactam and tetracycline in Campylobacter spp.isolated from broiler slaughterhouses in southern Brazil." Pesquisa Veterinária Brasileira 35, no. 7 (July 2015): 637–42. http://dx.doi.org/10.1590/s0100-736x2015000700006.

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Abstract The study was carried out to screen and analyze the genetic characteristics of antimicrobial resistance in Campylobacter spp. from poultry sources. A total of 141 strains of Campylobacter isolated from samples of broilers of slaughterhouses in southern Brazil was identified by phenotypic and genotypic methods. Campylobacter isolates were evaluated for its antimicrobial susceptibility and the presence of resistance genes. The strains were investigated for antimicrobial susceptibility against two agents (ampicillin and tetracycline) by disk diffusion method. PCR assay was used to confirm the specie and the presence of ampicillin (blaOXA-61), tetracycline tet(O), and the energy-dependent multi-drug efflux pump (cmeB) genes. Campylobacter jejuni was the most ubiquitous; its presence was determined in 140 samples out of 141 (99.3%), whereas Campylobacter coli was found only in one of the contaminated samples (0.70%). The results obtained showed 65% and 35.5% of Campylobacter isolates resistant to β-lactams and tetracyclines, respectively. The cmeB gene responsible for multidrug resistance was detected in 26 isolates out 141 strains (18.5%). Moreover, 36 out of 141 Campylobacter strains (25.6%) were found to be resistant to at least two different antimicrobia resistance markers (β-lactams and tetracyclines).

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Hunt Gerardo, S., D. M. Citron, M. C. Claros, and E. J. Goldstein. "Comparison of Etest to broth microdilution method for testing Streptococcus pneumoniae susceptibility to levofloxacin and three macrolides." Antimicrobial Agents and Chemotherapy 40, no. 10 (October 1996): 2413–15. http://dx.doi.org/10.1128/aac.40.10.2413.

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When the Etest was compared to broth microdilution for susceptibility testing of Streptococcus pneumoniae, levofloxacin, erythromycin, and penicillin results correlated for both methods; azithromycin and clarithromycin showed discrepancies of > or = 2 dilutions for 95.8% and 31.5% of the isolates, respectively. Levofloxacin was active against 141 of 142 isolates (< or = 2.0 micrograms/ml), making it a potentially useful new fluoroquinolone.

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K. S. Abbiramy, K. S. Abbiramy, and P. Ronald Ross P. Ronald Ross. "Determination of Acute Toxicity of NPK Fertilizers to Eisenia Foetida Using a Simple Paper Contact Method." International Journal of Scientific Research 2, no. 2 (June 1, 2012): 415–17. http://dx.doi.org/10.15373/22778179/feb2013/141.

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Giachetti, L. "A Simple Method for Treating Subgingival Class V Lesions." Operative Dentistry 44, no. 4 (July 1, 2019): 333–35. http://dx.doi.org/10.2341/18-141-t.

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SUMMARY Noncarious cervical lesions (NCCLs) are steadily increasing. Unfortunately, NCCL restorations represent one of the less durable types of restorations due to problems, including isolation and crevicular moisture control, especially in cases where the cervical margin is covered by soft tissue. This article presents a technique that allows us, through the use of a common metallic matrix, to isolate and make accessible class V cavities with intrasulcular cervical margins. The advantages and disadvantages of the technique are presented.

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Kamata, Arimichi, Taku Miyamae, Masaki Koizumi, Harigane Kohei, Hideki Sarukawa, Hiroshi Nemoto, and Koji Hino. "Using Computed Tomography Lymphography for Mapping of Sentinel Lymph Nodes in Patients with Breast Cancer." Journal of Clinical Imaging Science 11 (August 14, 2021): 43. http://dx.doi.org/10.25259/jcis_33_2021.

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Objectives: In breast cancer surgery, the combined use of the dye method and radioisotope (RI) method is recommended for identifying sentinel lymph nodes. However, the RI method is difficult to license, expensive, and difficult to introduce. Thus, we introduced computed tomography lymphography (CTLG) and investigated the characteristics and usefulness of CTLG. Material and Methods: Among breast cancer patients who underwent surgery during a 6-year period from January 2013 to December 2018, CTLG was performed on 141 patients with clinically negative lymph node metastasis. These cases were then retrospectively investigated. The number and location of lymph vessel, true sentinel lymph nodes, and the positional relationships with surrounding muscles and blood vessels were confirmed from the constructed 3D images. The actual surgeries were then performed using a dye method with indigo carmine based on images obtained using CTLG. Results: CTLG was able to identify lymph vessels and true sentinel lymph nodes in 131 of the 141 cases (92.91%). There were 97 patients in whom the first true sentinel lymph node reached from the breast was one node, 30 with two nodes, and 4 with three nodes. Moreover, there were three cases in which sentinel lymph nodes were present at Level II. During surgery, sentinel lymph nodes were identified in 131 patients (92.91%) using dye. Conclusion: CTLG has a high identification rate in sentinel lymph nodes, and it is considered a convenient and useful examination method because a lot of information, such as the number and position of sentinel lymph nodes, can be obtained.

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Szerlong, R. Glenn, and David E. Rundio. "A statistical modeling method for estimating mortality and abundance of spawning salmon from a time series of counts." Canadian Journal of Fisheries and Aquatic Sciences 65, no. 1 (January 1, 2008): 17–26. http://dx.doi.org/10.1139/f07-141.

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We present a statistical modeling method for estimating mortality and abundance of spawning salmon from time-series counts that eliminates the need for separate information about mortality. We model arrival and mortality using differential equations, where mortality can be constant or changing linearly, and estimate mortality and abundance from counts using maximum likelihood when multiple estimates of detection rate are available. We also develop an approximate likelihood to estimate mortality and abundance when only a single value for detection rate is available or to estimate only mortality when detection rates are entirely unknown. We demonstrate our approach using counts of coho salmon (Oncorhynchus kisutch) where mortality, abundance, and detection were determined from tagging at a weir. Our model for nonconstant mortality produced mortality estimates that closely matched the empirical data and were robust to variation in other parameters. It also provided a better fit to the stream counts and a closer abundance estimate to the weir count than the constant mortality model. Monte Carlo simulations indicated that the approximate likelihood provided reasonable estimates of mortality over most of the ranges of parameters explored, particularly under the nonconstant mortality model, and produced relatively unbiased abundance estimates using a single value for detection.

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Rioja, M., and R. Dodson. "HIGH-PRECISION ASTROMETRIC MILLIMETER VERY LONG BASELINE INTERFEROMETRY USING A NEW METHOD FOR ATMOSPHERIC CALIBRATION." Astronomical Journal 141, no. 4 (March 1, 2011): 114. http://dx.doi.org/10.1088/0004-6256/141/4/114.

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Liu, Pulin, Qiaoyun Huang, and Wenli Chen. "Heterologous expression of bacterial nitric oxide synthase gene: a potential biological method to control biofilm development in the environment." Canadian Journal of Microbiology 58, no. 3 (March 2012): 336–44. http://dx.doi.org/10.1139/w11-141.

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The nitric oxide synthase gene from Bacillus subtilis was heterologously expressed in Pseudomonas putida using a broad-host expression vector. Both the performance of the nitric oxide-specific fluorescent probe and the quantitative assessment of the nitric oxide end products demonstrated the generation of nitric oxide. The endogenous nitric oxide increased the motility of P. putida and decreased the capacity of P. putida and other multispecies bacteria to develop biofilms. On the practical side, endogenous nitric oxide offers an advantage in generating continuous, controllable levels of nitric oxide, which suggests a new method to use nitric oxide in the control of biofilms.

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Putranto, Thomas Triadi, Dian Agus Widiarso, and Fatir Yuslihanu. "Studi Kerentanan Air Tanah Terhadap Kontaminan Menggunakan Metode Drastic di Kota Pekalongan." Teknik 37, no. 1 (July 30, 2016): 26. http://dx.doi.org/10.14710/teknik.v37i1.9637.

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Kota Pekalongan berada di Pulau Jawa bagian Utara. Jumlah penduduk kota pekalongan setiap tahunnya mengalami peningkatan. Tahun 2008 jumlah penduduk Kota Pekalongan sebanyak 271.990 jiwa kemudian menjadi 290.347 di Tahun 2012.. Untuk memenuhi kebutuhan air, peduduk Kota Pekalongan menggunakan sumur gali yang menyadap air dari akuifer bebas atau akuifer dangkal. Air tanah bebas sangat rentan terhadap pencemaran akibat pengaruh letaknya yang dangkal dan aktivitas manusia. Oleh sebab itu diperlukan suatu kajian mengenai kerentanan air tanah terhadap kontaminan. Analisis dilakukan denganmenggunakan metode DRASTIC. Metode ini merupakan metode pombobotan berdasarkan beberapa parameter, yaitu: kedalaman muka air tanah (D), jumlah area recharge (R), litologi akuifer (A), jenis media tanah (S), topografi (T), jenis media zona tak jenuh air (I), dan konduktivitas hidrolika (C). Berdasarkan hasil analisis DRASTIC, terdapat tiga tingkat kerentanan yaitu: daerah tingkat kerentanan sedang dengan nilai DRASTIC Indeks (DI) 101-140, daerah tingkat kerentanan tinggi dengan nilai DI 141-180, dan daerah tingkat kerentanan sangat tinggi dengan nilai DI >180.[An Assessment of Groundwater Vulnerability of Contaminant Using DRASTIC Method in Pekalongan City] Pekalongan city which located on the north of Java Island is growing fast, in particular in the population growth. In 2008, total population in Pekalongan city was 271.990 inhabitants increased then up to 290.347 inhabitants in 2012. To fill the water necessary, people in Pekalongan city are using dug wells which are abstracted groundwater from the unconfined aquifer or shallow aquifer. Shallow groundwater can be vulnerabe which are influenced by surface and human activities. Thus, it requires an assessment of the groundwater vulnerability and risk of contaminant. It was analyzed by DRASTIC method. The DRASTIC method is applied by using weighted of some parameters, i.e.: groundwater Depth (D), amount of Recharge (R), Aquifer type (A), Soil type (S), topography (T), Impact of unsatuated zone (I), and hydraulic Conductivity (C). DRASTIC Index (DI) results in three vulnerability levels which are medium with DI 101-140, high with DI around 141-180 and above 180 for high vulnerable of contaminant.

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Bitskinashvili, Kakha, Inga Gabriadze, Tamara Kutateladze, Boris Vishnepolsky, David Mikeladze, and Nelly Datukishvili. "Influence of Heat Processing on DNA Degradation and PCR-Based Detection of Wild-Type and Transgenic Maize." Journal of Food Quality 2019 (July 4, 2019): 1–11. http://dx.doi.org/10.1155/2019/5657640.

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Reliable detection of genetically modified (GM) maize is significant for food authenticity, labelling, quality, and safety assessment. This study aims to evaluate the factors influencing degradation and polymerase chain reaction (PCR) amplification of DNA from the wild type and transgenic maize (events Bt-176 and MON810) during thermal treatment at 100°C and 121°C. A new PCR method was developed targeting the Cry1Ab gene to detect insect-resistant GM plants. The yield of genomic DNAs extracted by the DNeasy plant mini kit dramatically decreased while DNAs obtained by cetyltrimethyl ammonium bromide- (CTAB-) based method did not show any visible changes in the yield by the time of processing. Treatment at 100°C did not significantly affect either genomic DNAs or amplicons. Heating at 121°C induced time-dependent degradation of genomic DNAs and exogenous Cry1Ab gene; however, it did not have any considerable influence on the exogenous 141 bp amplicons or endogenous amplicons in the range of 102 bp to 226 bp with the exception of the event MON810 extracted by the DNeasy plant mini kit. More yield was observed at 226 bp than 140 bp fragment of the invertase gene. The 141 bp fragment of the transgenic CaMV 35S promoter exhibited the highest thermal stability of all the examined amplicons. Analysis of foodstuffs demonstrated 102 bp amplicons specific for the zein gene as the effective marker to detect maize in the processed foods. The obtained results demonstrate that PCR-based detection of the wild type and transgenic maize is dependent on the combination of different parameters of crucial factors such as temperature and duration of exposure, transgenic event, DNA extraction method, DNA marker, and size and location of amplicons.

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Vitchuk, Pavel, and Dmitriy Mokin. "METHOD OF CALCULATION OF THE ELEVATOR SPEED LIMITER WITH THE INERTIAL ROLLER." Vestnik MGSU, no. 7 (July 2016): 134–41. http://dx.doi.org/10.22227/1997-0935.2016.7.134-141.

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NAKAGATA, Naomi, Toru TAKEO, Kiyoko FUKUMOTO, Yukie HARUGUCHI, Tomoko KONDO, Yumi TAKESHITA, Yuko NAKAMUTA, Tomoko UMENO, and Shuuji TSUCHIYAMA. "Rescue In Vitro Fertilization Method for Legacy Stock of Frozen Mouse Sperm." Journal of Reproduction and Development 60, no. 2 (2014): 168–71. http://dx.doi.org/10.1262/jrd.2013-141.

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Kasyev, N. B., M. S. Aytnazarov, A. A. Kazieva, and A. N. Nurbekova. "Immunochemiluminescence in the diagnosis of relapsing froms of echinococcosis." Kazan medical journal 98, no. 1 (February 15, 2017): 141–43. http://dx.doi.org/10.17750/kmj2017-141.

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Aim. To improve immunologic diagnosis of relapsing and residual echinococcosis we used the novel chemiluminescent method based on registration of changes of biologic fluids emmision in normal and pathologic conditions.Methods. We observed 136 patients with relapsing echinococcosis, 109 patients with primary echinococcosis and 91 patients with residual cavities after echinococcectomy. As a control we included 186 patients with other diseases such as hepatitis, liver cirrhosis, liver and lung cancer, cholecystitis and others having some clinical similarity with echinococcosis and requiring differential diagnosis, and also 30 donors were included. Besides, 111 people previously operated for echinococcosis (6 month to 10 years prior) were examined.Results. In comparison of immunochemiluminescence with well-known serologic test this method demonstrated some benefits: short time to receiving the results, small amount of the blood necessary for the analysis (0.02 ml), high specificity and sensitivity. As the time period after the surgery increases, intensity of luminescence in immunochemiluminescence gradually decreases in case of no relapse and increases in relapse.Conclusion. Immunochemiluminescence of the whole blood is a valuable diagnostic method; this method should be included to diagnostic workup for patients with suspected echinococcosis; immunochemiluminescence provides the opportunity to define pathology and radicality of performed surgery and to reveal the disease relapse.

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Saputra, Moch Fitriawan Eka, and Muhammad Rizky. "Peramalan Jumlah Kasus Infeksi Saluran Pernafasan Akut (ISPA) pada Laki-Laki Tahun 2019 dengan Metode ARIMA." Jurnal Biometrika dan Kependudukan 8, no. 2 (December 10, 2019): 138. http://dx.doi.org/10.20473/jbk.v8i2.2019.134-141.

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The ARIMA method is an approach that forms the most powerful model in analyzing time series data, and the studies given are very thorough. This method can be modeling data stationary or not stationary, it can be seen from sine wave shape of the plot ACF. This method is used because obtained the results are better and more accurate. According to WHO, Acute Respiratory Infection (ARI) is an infectious disease that causes can be morbidity and mortality. A four million people die each year. This study used secondary data so that it is categorized as non reactive research. The population were cases of Acute Respiratory Infections (ARI) at Jagir Health Center Surabaya which were recorded in 2013 to 2018 (monthly). The dependent variable is the cases of Acute Respiratory Infection (ARI), while the independent variable is time. The model that was obtained from the ARIMA method is a model (2.0,1). The forecasting result is 354 cases in 2019, the forecasting has increased from 2018 to only 313 cases. It was a suggestion that the forecasting result can be a reference for developing a policy and a new program or improvement in previous program so that the number cases of ARI at the Jagir Health Center can be resolved properly.

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Волоський, В. М., С. В. Шолом, and В. В. Чумак. "Enhancement of sensitivity of retrospective thermoluminescent dosimetry method on the base of quartz." Scientific Herald of Uzhhorod University.Series Physics 10 (December 31, 2001): 138–41. http://dx.doi.org/10.24144/2415-8038.2001.10.138-141.

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Vaira, Dino, and Federico Perna. "141 A 20 Hours ELISA Method Chemosusceptibility Rapid Test for Helicobacter pylori." Gastroenterology 134, no. 4 (April 2008): A—25. http://dx.doi.org/10.1016/s0016-5085(08)60123-7.

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Pahlevani, Farshid, Koichi Anzai, and E. Niyama. "Quick Semi-Solid Slurry Making Method Using Metallic Cup." Solid State Phenomena 141-143 (July 2008): 463–68. http://dx.doi.org/10.4028/www.scientific.net/ssp.141-143.463.

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The needs for high-strength and light weight structural materials have increased in automotive and aerospace structure applications. The semi-solid processed light alloys inherently offer the opportunity to produce high integrity components for these requirements. Various processing methods exist for applying agitation to a molten metal during solidification to obtain metal slurries suitable for semi-solid metal processing. In this paper, a new technique (Cup-Cast method) to achieve semi-solid metal structure using agitation and direct spherical growth during solidification is reported. Cup-Cast method is the most quick and simple semi-solid processing route which semi-solid slurry would be prepared just by pouring molten metal into a metallic cup. In this study Cup-Cast method was introduced and effect of process parameters on micro-structural characterization of slurry prepared by this method was investigated.

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Sabban, Alfia, Dominggus Rumahlatu, and Theopilus Watuguly. "POTENSI EKSTRAK DAUN TERATAI (Nymphaea pubescens L.) DALAM MENGHAMBAT Staphylococcus aureus." BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan 3, no. 2 (March 20, 2017): 129–41. http://dx.doi.org/10.30598/biopendixvol3issue2page129-141.

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Background: The lotus (Nymphaea pubescens L.) is one of the ornamental plants that many people in demand because it has varieties, shapes, and color of flowers are diverse. The lotus has the potential to be developed as an antibacterial. This study aims to determine the use of lotus leaf extract as anti-bacterial Staphylococcus aureus. Method: This study included the making of extraction using maceration method using ethanol, phytochemical testing of lotus leaf extract and testing of anti bacterial activity was done by diffusion method agar by observing and measuring the inhibition zone diameter formed on Muller Hinton (MHA) media. Then done by giving lotus leaf extract with 3 treatment of concentration that is 5%, 10%, 20%, positive control (ampicillin) and negative control (aquades). With an incubation period of 1 x 24 hours. Result: The result of phytochemical test showed that lotus leaf extract contain alkaloid compound, triterpenoid, steroid, flavonoid, phenolic and saponin. Anti-bacterial activity test results showed that the concentration given was not able to inhibit the growth of test bacteria. Conclusions: Bacteriostatic tests with a 60% extract concentration indicating that at 10-5 to 10-9 dilutions there were no bacterial colonies growing.

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Smerkova, Kristyna, Tomas Rypar, Vojtech Adam, and Marketa Vaculovicova. "Direct Magnetic Bead-Based Extraction of MicroRNA from Urine with Capillary Electrophoretic Analysis Using Fluorescence Detection and Universal Label." Journal of Biomedical Nanotechnology 16, no. 1 (January 1, 2020): 76–84. http://dx.doi.org/10.1166/jbn.2020.2872.

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Short non-coding RNAs, specifically microRNAs (miRNAs), are of a great interest due to their presumed function in genome regulation. Moreover, miRNAs are currently perceived as potential biomarkers for numerous diseases; a variety of detection methods and sensing systems have therefore been studied. We present a magnetic-bead-based assay for specific miRNA isolation coupled with sensitive electrophoretic analysis with fluorescence detection. The magnetic separation step involves creating a duplex with targeted miR-141, which is subsequently cleaved from the magnetic bead surface with a specific endonuclease. The duplex is then determined using capillary electrophoresis with laser-induced fluorescence detection in the presence of the fluorescent dye PicoGreen for quantitating double-stranded DNA. The benefits of using microcolumn separation technique coupled with sensitive detection over traditionally used determination by fluorescence spectrometry include the fact that there is no need for a specific pre-labeled fluorescent probe. This significantly simplifies the method and reduces the costs. Cross-reactivity with mismatched oligonucleotides (3 and 5 mismatched bases) and different miRNAs (miR-124 and miR-150) was tested, demonstrating the specificity of the developed method for miRNA-141. This magnetic extraction method was demonstrated for the direct isolation and determination of miR-141 at different concentration levels from urine samples and the achieved nanomolar detection limit.

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Khegay, S. V., A. K. Dzhaparova, E. M. Dushenalieva, B. Kalmyrzaev, K. T. Kasymbekova, T. E. Kuchuk, N. T. Usenbaev, and A. T. Zhunushov. "Molecular Diagnostics of Coronavirus Infection in the Kyrgyz Republic." Problems of Particularly Dangerous Infections, no. 2 (July 12, 2020): 141–43. http://dx.doi.org/10.21055/0370-1069-2020-2-141-143.

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Abstract. Objective of the study was to develop an effective method of sample pooling for the detection of SARSCoV-2 coronavirus RNA using PCR and evaluate that approach with various test systems.Materials and methods. SARS-CoV-2 coronavirus RNA was detected in samples containing nasal swabs placed in a transport medium. 5 samples were combined into one pool to perform the analysis. The effectiveness of the “in single test tube” pooling method for performing mass studies for COVID-19 was evaluated using the Vector-PCRrv-2019-nCoV-RG-19 test systems,Russia; “ArtTest COVID-19”,Belarus; “BioSpeedy”,Turkey.Results and discussion. A total of 587 pools were studied, consisting of 2935 test samples, in which 56 samples containing SARS-CoV-2 RNA were detected and confirmed by PCR. When studying the method of pooling samples, its specificity and optimal sensitivity for detecting SARS-CoV-2 RNA using the Vector-PCRrv-2019-nCoV-RG, ArtTest COVID-19, and BioSpeedy test systems were shown. The results of applying the pooling method correlated with the data obtained without pooling samples. The average deviation of the cycle amounted to 2 Ct; the fluorescence curve of positive samples corresponded to the «S» form.

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EDWARDS, GENEVIEVE, MARLENE JANES, LUCINA LAMPILA, and JOHN SUPAN. "Consumer Method To Control Salmonella and Listeria Species in Shrimp." Journal of Food Protection 76, no. 1 (January 1, 2013): 59–64. http://dx.doi.org/10.4315/0362-028x.jfp-12-141.

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The purpose of this study was to determine whether the current consumer method of boiling shrimp until floating and pink in color is adequate for destroying Listeria and Salmonella. Shrimp samples were submerged in bacterial suspensions of Listeria and Salmonella for 30 min and allowed to air dry for 1 h under a biosafety cabinet. Color parameters were then measured with a spectrophotometer programmed with the CIELAB system. Twenty-four shrimp samples were divided into groups (days 0, 1, or 2) and stored at 4°C. The samples were treated by placing them in boiling water (100°C) on days 0, 1, and 2. The shrimp were immediately removed from the boiling water once they floated to the surface, and color parameters were measured. Bacterial counts were determined, and the log CFU per gram was calculated. The effect of sodium tripolyphosphate on the color change of cooked shrimp also was determined. Initial bacterial counts on shrimp after air drying were 5.31 ± 0.14 log CFU/g for Salmonella Enteritidis, 5.24 ± 0.31 log CFU/g for Salmonella Infantis, 5.40 ± 0.16 log CFU/g for Salmonella Typhimurium, 3.91 + 0.11 log CFU/g for Listeria innocua, 4.45 ± 0.11 log CFU/g for Listeria monocytogenes (1/2a), and 3.70 ± 0.22 log CFU/g for Listeria welshimeri. On days 0, 1, and 2, all bacterial counts were reduced to nondetectable levels for shrimp samples that floated. The average time for shrimp to float was 96 ± 8 s. The bacterial counts remained at nondetectable levels (<10 log CFU/g) during refrigerated (4°C) storage of cooked shrimp for 2 days. The redness, yellowness, and lightness were significantly higher (P < 0.0001) for the cooked shrimp than for the uncooked shrimp on all days tested. The standard deviation for redness in the cooked shrimp was large, indicating a wide range of pink coloration on all days tested. The results suggest that boiling shrimp until they float will significantly reduce Listeria and Salmonella contamination, but color change is not a good indication of reduction of these pathogens because of the wide natural color variation.

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Хасанов, Р. Р., А. А. Гумеров, К. Х. Шефер, and Л. М. Вессель. "A method for culturing of enteric nervous system cells suitable for tissue engineering of the intestine." ZHurnal «Patologicheskaia fiziologiia i eksperimental`naia terapiia», no. 2() (May 27, 2019): 132–41. http://dx.doi.org/10.25557/0031-2991.2019.02.132-141.

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Лечение синдрома короткой кишки является сложной проблемой в современной медицине. У ряда пациентов существующие методы лечения неэффективны, а трансплантация кишечника показывает неудовлетворительные результаты. Тканевая инженерия тонкой кишки может быть инновационным методом лечения этих пациентов. Одним из ключевых элементов в создании кишки является выращивание нервной системы кишечника. До настоящего времени не описаны культуры нервных клеток, выделенных из нервной системы кишечника, выращенные в трёхмерном матриксе на основе гиалуроновой кислоты. Цель исследования: разработать метод выращивания in vitro взаимодействующих друг с другом клеток нервной системы кишечника в трехмерной среде. Методы: клетки нервной системы кишечника выделялись из кишечника крыс путём препарирования и ферментативного воздействия на мышечный слой тонкой кишки. Далее клетки помещались в трёхмерный матрикс и культивировались в нём в течении нескольких дней. Результаты. Клетки нервной системы кишечника были выделены из крыс и культивировались в трёхмерном матриксе, помещённом в раствор для культур клеток с добавлением специфических факторов роста. Микроскопирование культур клеток на восьмые сутки показало, что нервные клетки активно растут, соединяясь с другими нервными клетками при помощи отростков. Конфокальная микроскопия в сочетании с иммуногистохимическим окрашиванием клеток специфичным маркером нервных клеток показала, что полученные культуры действительно состоят из нервных клеток. Заключение. Разработанный метод позволяет вырастить in vitro живущие и взаимодействующие друг с другом клетки нервной системы кишечника в трехмерной среде. Полученная культура клеток может быть использована для моделирования стенки тонкой кишки путем тканевой инженерии. Background. Treatment of short bowel syndrome is a complex issue in modern medicine. Existing treatment methods are inefficient in some cases, and bowel transplantation shows unsatisfactory results. Tissue engineering of the small intestine can represent an innovative method of treatment for these patients. One of the key elements in creation of the gut is culturing of enteric nervous system cells. There is no description of the nerve cells culture extracted from the enteric nervous system and grown in a hyaluronic-acid based three-dimensional matrix so far. Research objective: establishing an in vitro method for culturing of interacting enteric nervous system cells in a 3D environment. Methods. The enteric nervous system cells used for these experiments were isolated from Sprague-Dawley rats. Hyaluronic-acid based hydrogel HyStem®-C (ESI BIO - A Division of BioTime, USA) was used as a 3D matrix. Enteric nervous system cells were isolated from 5- to 8-day-old newborn Sprague-Dawley rats. Animals were decapitated with a rodent guillotine (cervical spinal cord transection). After that they were laparotomized, and their intestines were isolated. The removed intestines were placed in a Petri dish filled with the MEM (Minimum Essential Medium) supplemented with antibiotics (25 µl factory-produced solution of gentamycin (40 mg/ml) and 50 µl of metronidazole solution (5 mg/ml) per 50 ml of MEM). The small intestine was then separated from the colon and mesentery under an optical microscope, and the muscular layer of small intestine was isolated from the submucosal layer. The isolated muscular layer was placed in a tube with the deoxyribonuclease and collagenase solution as well as Balanced Salt Solution and incubated for 2 h at 37°C and 5% CO2. As a result, the intestinal muscle tissue was broken down completely in the produced solution, while areas of the myenteric plexus remained intact and were recognizable under the microscope as a network. These neural networks were treated with a trypsin solution. Further, after mechanical processing, a suspension of nerve cells was obtained. The hyaluronic-acid based gel HyStem®-C (ESI BIO - A Division of BioTime, Inc., USA) with added collagen was used to produce a 3D matrix. The enteric nervous system cells were added to this three-dimensional matrix and stirred. Matrix had been hardening for 30 minutes. The cell cultures were fixed, stained and microscoped 10-21 days later. For immunofluorescence staining of cells we used a direct immunohistochemical method with the anti-ß III Tubulin antibody conjugated with the fluorochrome Alexa 488 Flour (Merck Millipore). Anti-ß III Tubulin is a specific antibody used for peculiar staining of neurons. The anthraquinone dye with a high affinity to double-stranded DNA - DRAQ5 (Thermo Fisher Scientific) was applied in order to identify cell nuclei. Microscopy was conducted with Leica TCS SP8 (Leica, Germany). Results. The technique we represented allowed us to produce a culture of enteric nervous system cells isolated from rats in the three-dimensional matrix. Confocal microscopy combined with immunohistochemical staining with specific neuronal marker showed that the final cultures indeed consisted of nerve cells. Larger nerve cell clusters were encountered. Herewith, some neurons were arranged close to each other while others were located at a distance; however, all neurons were interconnected. Neurons also clearly showed wide processes morphologically similar to those of axons. In addition, many thin and branching processes resembling dendrites morphologically were visible. The use of confocal microscopy allowed to receive a series of images at different depths of the focal plane, which could then be reconstructed to a three-dimensional image. The 3D reconstruction of the nervous plexi clearly revealed neurons joined together in a complex network. For DRAQ5 stains all cell nuclei, not just those of neurons, the presence of stained cell nuclei just “hanging” on the reconstruction without cytosomes of nerve cells indicated that other cell types were also present in this texture. The present work showed a possibility for culturing interconnected enteric nervous system cells as well as nerve plexi within the three-dimensional medium in vitro. The key step in growing the enteric nervous system cells for both two-dimensional and three-dimensional medium claims to be the characteristics of functional cellular activity. Following stages of the study require observing of the cells’ functionality, for example, by using the analysis of intracellular Ca 2+ concentration (calcium imaging) and its variability. In addition, the study of cellular interactions within the enteric nervous system with other types of cells in the three-dimensional medium also encourages a great interest. Conclusion. The developed method enables one to culture the interconnected cells of the enteric nervous system within the three-dimensional medium in vitro, which can be used to create the enteric nervous system when growing the small intestinal wall by means of tissue engineering. Further research is based on studying the functionality of cells grown in a three-dimensional medium as well as on co-culturing of enteric nerve cells with other types of cells in three-dimensional media.

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Khan, S. G., L. K. Ibrayeva, N. V. Syabina, and Z. M. Yuldashev. "RESEARCH AND APPLICATION OF METHODS FOR CALCULATING THE MEASUREMENT UNCERTAINTY OF INDUSTRIAL FLOWMETERS." NEWS of National Academy of Sciences of the Republic of Kazakhstan 6, no. 444 (December 15, 2020): 145–53. http://dx.doi.org/10.32014/2020.2518-170x.141.

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To create conditions for the recognition of Kazakhstani certificates of conformity and the results of product tests, an assessment of measurement uncertainty is required. In this regard, there has been an increase in the practical application in Kazakhstan of the concept of measurement uncertainty. The authors developed a physical stand for a mobile complex designed to verify electromagnetic flowmeters at the place of operation. To obtain verification results, programs were developed to calculate the uncertainty of an electromagnetic flowmeters using the NI LabView software. In addition, a model for estimating the uncertainty of the relative error of flowmeters was proposed, and the measurement uncertainty was estimated using three methods: standard, Monte Carlo and Kragten. Finally, a comparative analysis was conducted on the results of the estimation of the uncertainty of the relative error of the industrial electromagnetic flowmeter. All methods give standard uncertainty values that do not exceed the acceptable range of relative error (± 1%). However, Monte Carlo method gives better results for sufficiently large number of simulations. No significant differences between the results obtained using standard and Kragten methods were discovered. The Kragten method is preferable in the absence of the need to calculate the sensitivity coefficients when calculating the total standard uncertainty, which is important for complex measurement equations.

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Stockwell, J. D., and O. E. Johannsson. "Temperature-dependent allometric models to estimate zooplankton production in temperate freshwater lakes." Canadian Journal of Fisheries and Aquatic Sciences 54, no. 10 (October 1, 1997): 2350–60. http://dx.doi.org/10.1139/f97-141.

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Empirical relationships between body size and temperature, and daily P/B of temperate freshwater zooplankton species were derived from the literature. Body size explained 81% of the variation in daily P/B of zooplankton at temperatures <<= 10°C and 48% at temperatures >10°C. The two relationships did not differ in slope but significantly differed in elevation. The regression models were then applied to zooplankton samples collected from a midlake station in Lake Ontario to predict zooplankton seasonal production. Production estimates from the regression models, when compared with estimates from the egg ratio method for the same data set, were similar for cladoceran species, four and 10 times higher for two copepod species, and two times higher for total zooplankton production. In general, these differences are within the range expected based on previous studies that compared different production models. The regression models can provide quick, independent estimates of zooplankton production to complement estimates based on traditional, species-specific methods. Furthermore, the models can be used in conjunction with automated synoptic instruments, such as optical plankton counters and temperature probes, to provide production estimates that better represent large, complex systems such as the Great Lakes.

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Pelletier, Luc, and Charles J. Krebs. "Line-transect sampling for estimating ptarmigan (Lagopus spp.) density." Canadian Journal of Zoology 75, no. 8 (August 1, 1997): 1185–92. http://dx.doi.org/10.1139/z97-141.

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Current methods of estimating ptarmigan density require total counts, and hardly apply to areas exceeding 1 km2. We evaluated the applicability, accuracy, and efficiency of line-transect sampling as an alternative method for estimating breeding density of male ptarmigan. We compared Hayne's and generalized Hayne's estimators with line-transect estimators based on perpendicular distance. We surveyed 6 subalpine areas in Kluane, Yukon, with 231 km of transects in 1995 and 1996, in addition to a 77-ha grid where we also conducted total counts. Estimates of perpendicular distance were accurate (bias = −3 to −7%). Their efficiency resides mainly in detecting changes in density over 2-year periods in highly or moderately fluctuating populations. Performance of Hayne's estimator was close to that of perpendicular distance (coefficient of correlation, r = 0.95), even if in many cases the average sighting angle was not 32.7°. The generalized Hayne's estimator was not robust; biases were up to −90%. The results of this survey, with densities ranging between 0 and 65 males/km2 for similar and adjoining subalpine areas, dispute the principle that the "health" of a population can be assessed by censusing only a small area.

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Huo, Congde, Guoqing Shi, Wai Har Lam, Di Chen, Quizhi Cindy Cui, Q. Ping Dou, and Tak Hang Chan. "Semi-synthesis and proteasome inhibition of D-ring deoxy analogs of (–)-epigallocatechin gallate (EGCG), the active ingredient of green tea extract." Canadian Journal of Chemistry 86, no. 6 (June 1, 2008): 495–502. http://dx.doi.org/10.1139/v07-141.

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A semi-synthetic route to the D-ring analogs of (–)-epigallocatechin gallate (EGCG) from the relatively abundant (–)-epigallocatechin (EGC), isolated from green tea leaves, is described. A natural product (13), found in Cistus salvifolius, its acetate (14) and analog (17) were synthesized by this method. Their inhibitory activities against proteasomes were investigated.Key words: green tea, (–)-epigallocatechin gallate (EGCG), (–)-epigallocatechin (EGC), proteasome inhibition.

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Ye, Xin, Junfeng Ma, Zhao Li, and Liming Zhu. "A Reachability Verification Method Based on Pi- Calculus and Role Network Model for Cross- Organizational Business Processes." International Journal of Electrical Energy 1, no. 3 (2013): 137–41. http://dx.doi.org/10.12720/ijoee.1.3.137-141.

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Campos-Giménez, Esther, Patric Fontannaz, Karine Redeuil, Tamara Kilinc, and Dawn Dowell. "Vitamin C in Infant Formula and Adult/Pediatric Nutritional Formula by Ultra-Performance Liquid Chromatography with Ultraviolet Detection: First Action 2012.22." Journal of AOAC INTERNATIONAL 96, no. 5 (September 1, 2013): 1065–67. http://dx.doi.org/10.5740/jaoacint.13-141.

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Abstract During the AOAC Annual Meeting held in Las Vegas, NV from September 30 to October 3, 2012, the Stakeholder Panel on Infant Formula and Adult Nutritionals convened to review single-laboratory validation data submitted for the method, Vitamin C in Adult/Pediatric Formula by Ultra-Performance Liquid Chromatography with Ultraviolet Detection. This method is a modified version of the method "HPLC-UV Determination of Total Vitamin C in a Wide Range of Fortified Food Products" previously published in Food Chem., 94, 626–631 (2006). The SLV data from the modified method were reviewed and compared to the standard method performance requirements (SMPR 2012.012), and it was concluded that the method meets the requirements. The method was approved as AOAC Official First Action. The method is based on the acidic extraction of ascorbic acid in the presence of Tris[2-carboxyethyl] phosphine (TCEP) as a reducing agent. Separation was achieved on a C18 column with a sodium acetate eluent (pH 5.4) combined with TCEP and decylamine as an ion-pairing agent. Accuracy rates were between 90 and 100%. Repeatability RSD (RSDr) ranged from 1.4 to 2.5%, and intermediate reproducibility RSD (RSDiR) ranged from 1.3 to 7.5%.

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Zakharova, I. N., I. M. Osmanov, E. B. Machneva, E. B. Mumladze, N. V. Gavelya, O. V. Brazhnikova, and I. N. Lupan. "Clinical urine analysis: a role in modern paediatric practice." Medical Council, no. 2 (February 16, 2019): 131–41. http://dx.doi.org/10.21518/2079-701x-2019-2-131-141.

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Urine testing has not only helped physicians to diagnose various diseases since ancient times, but has also stimulated the emergence of such areas of medicine as urology, nephrology, endocrinology. The method itself has evolved over the centuries from purely visual (uroscopy) to modern, physico-chemical, biochemical and microscopic examination of urine. Despite the emergence in recent years of new accurate and more advanced methods for the study of biological fluids, clinical analysis of urine remains one of the most frequently performed studies in the practice of a doctor, including a paediatrician. This relatively simple and quick analysis is a screening method for the diagnosis of diseases of the urinary system, as well as many other organs and systems. The article describes the current aspects of this laboratory study, which is still one of the most important in the arsenal of a pediatrician.

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Parashchuk, Lidiya, Viktoria Kochubei, Petro Novosad, Bohdan Seredyuk, and Andriy Hamarnik. "Modified fine-grained concrete for floor heating system." French-Ukrainian Journal of Chemistry 8, no. 1 (2020): 133–41. http://dx.doi.org/10.17721/fujcv8i1p133-141.

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This work shows the influence of components and hardening conditions on linear shrinkage of fine-grain concrete. The floor heating system is one of the most popular options of the heating system these days. The possibility of using this composition for screed for floor heating system is shown. A method of enhancing of strength of the concrete by adding reinforcing fibers and plasticizer has been suggested. Concrete composition has been optimized.

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Brunel, Dominique, Nicole Froger, and Georges Pelletier. "Development of amplified consensus genetic markers (ACGM) in Brassica napus from Arabidopsis thaliana sequences of known biological function." Genome 42, no. 3 (June 1, 1999): 387–402. http://dx.doi.org/10.1139/g98-141.

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A method for the development of consensus genetic markers between species of the same taxonomic family is described in this paper. It is based on the conservation of the peptide sequences and on the potential polymorphism within non-coding sequences. Six loci sequenced from Arabidopsis thaliana, AG, LFY3, AP3, FAD7, FAD3, and ADH, were analysed for one ecotype of A. thaliana, four lines of Brassica napus, and one line for each parental species, Brassica oleracea and Brassica rapa. Positive amplifications with the degenerate primers showed one band for A. thaliana, two to four bands in rapeseed, and one to two bands in the parental species. Direct sequencing of the PCR products confirms their peptide similarity with the "mother" sequence. By comparison of intron sequences, the correspondence between each rapeseed gene and its homologue in one of the parental species can be determined without ambiguity. Another important result is the presence of a polymorphism inside these fragments between the rapeseed lines. This variability could generally be detected by differences of electrophoretic migration on long non-denaturing polyacrylamide gels. This method enables a quick and easy shuttle between A. thaliana and Brassica species without cloning.Key words: consensus genetics markers, PCR specific, Brassica, Arabidopsis, targeted markers, DSCP.

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Wilson, Arlette C., and Kimberly Key. "Revised Accounting For Business Combinations." American Journal of Business Education (AJBE) 1, no. 2 (December 1, 2008): 13–20. http://dx.doi.org/10.19030/ajbe.v1i2.4620.

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The Financial Accounting Standards Board (FASB) has recently issued Statement of Financial Accounting Standards No. 141 (Revised 2007) Business Combinations. The object of this Statement is to improve the relevance, representational faithfulness, and comparability of reported information about a business combination and its effects. This Statement replaces FASB Statement No. 141, but retains the fundamental requirements that the acquisition method of accounting (previously called the purchase method) be used for all business combinations. Some of the changes related to the accounting for business combinations as a result of the new requirements are discussed and illustrated below.

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Refardt, Julie, Clara Odilia Sailer, Irina Chifu, Bettina Winzeler, Ingeborg Schnyder, Martin Fassnacht, Wiebke Fenske, Mirjam Christ-Crain, and _. _. "The challenges of sodium measurements: indirect versus direct ion-selective method." European Journal of Endocrinology 181, no. 2 (August 2019): 193–99. http://dx.doi.org/10.1530/eje-19-0101.

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Background Diagnosis and treatment of dysnatremia is challenging and further complicated by the pitfalls of different sodium measurement methods. Routinely used sodium measurements are the indirect (plasma/serum) and direct (whole blood) ion-selective electrode (ISE) method, showing discrepant results especially in the setting of acute illness. Few clinicians are aware of the differences between the methods in clinically stable patients or healthy volunteers. Methods Data of 140 patients and 91 healthy volunteers undergoing osmotic stimulation with hypertonic saline infusion were analyzed. Sodium levels were measured simultaneously by indirect and direct ISE method before and at different time points during osmotic stimulation up to a sodium threshold of ≥150 mmol/L. The primary outcome was the difference in sodium levels between the indirect and direct ISE method. Results 878 sodium measurements were analyzed. Mean (s.d.) sodium levels ranged from 141 mmol/L (2.9) to 151 mmol/L (2.1) by the indirect ISE compared to 140 mmol/L (3) to 149 mmol/L (2.8) by the direct ISE method. The interclass correlation coefficient between the two methods was 0.844 (95% CI: 0.823–0.863). On average, measurements by the indirect ISE were 1.9 mmol/L (95% CI limits: −3.2 to 6.9) higher than those by the direct ISE method (P < 0.001). The tendency of the indirect ISE method resulting in higher levels increased with increasing sodium levels. Conclusion Intra-individual sodium levels differ significantly between the indirect and direct ISE method also in the absence of acute illness. It is therefore crucial to adhere to the same method in critical situations to avoid false decisions due to measurement differences.

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Peterson, Lance R., Stephen A. Young, Thomas E. Davis, Zi-Xuam Wang, John Duncan, Christopher Noutsios, Oliver Liesenfeld, John C. Osiecki, and Michael A. Lewinski. "Evaluation of the cobas Cdiff Test for Detection of Toxigenic Clostridium difficile in Stool Samples." Journal of Clinical Microbiology 55, no. 12 (September 27, 2017): 3426–36. http://dx.doi.org/10.1128/jcm.01135-17.

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ABSTRACTNucleic acid amplification tests (NAATs) are reliable tools for the detection of toxigenicClostridium difficilefrom unformed (liquid or soft) stool samples. The objective of this study was to evaluate performance of the cobas Cdiff test on the cobas 4800 system using prospectively collected stool specimens from patients suspected of havingC. difficileinfection (CDI). The performance of the cobas Cdiff test was compared to the results of combined direct and broth-enriched toxigenic culture methods in a large, multicenter clinical trial. Additional discrepancy analysis was performed by using the XpertC. difficileEpi test. Sample storage was evaluated by using contrived and fresh samples before and after storage at −20°C. Testing was performed on samples from 683 subjects (306 males and 377 females); 113 (16.5%) of 683 subjects were positive for toxigenicC. difficileby direct toxigenic culture, and 141 of 682 subjects were positive by using the combined direct and enriched toxigenic culture method (reference method), for a prevalence rate of 20.7%. The sensitivity and specificity of the cobas Cdiff test compared to the combined direct and enriched culture method were 92.9% (131/141; 95% confidence interval [CI], 87.4% to 96.1%) and 98.7% (534/541; 95% CI, 97.4% to 99.4%), respectively. Discrepancy analysis using results for retested samples from a second NAAT (Xpert C. difficile/Epi test; Cepheid, Sunnyvale, CA) found no false-negative and 4 false-positive cobas Cdiff test results. There was no difference in positive and negative results in comparisons of fresh and stored samples. These results support the use of the cobas Cdiff test as a robust aid in the diagnosis of CDI.

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Грицай, О. О., С. П. Волковецький, and В. А. Лібман. "Research of influence of detector load at cross-section determination after transmission method by the filtered reactor beams." Scientific Herald of Uzhhorod University.Series Physics 30 (December 31, 2011): 135–41. http://dx.doi.org/10.24144/2415-8038.2011.30.135-141.

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Curt, Corinne, V. Leblanc, I. Allais, N. Perrot, V. Chevallereau, and Gilles Trystram. "Determination of functioning conditions for the meat emulsification process using at-line human evaluations and the Simplex method." Sciences des Aliments 23, no. 1 (February 28, 2003): 138–41. http://dx.doi.org/10.3166/sda.23.138-141.

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Alnavis, Noviana Bayu, Dwi Nowo Martono, and Udi Syahnoedi Hamzah. "Recommendations for Improving the ISO 14001 Certification Based on the Company’s Perception Analysis of the Certification Obstacles and Benefits." JURNAL KESEHATAN LINGKUNGAN 13, no. 3 (July 31, 2021): 132. http://dx.doi.org/10.20473/jkl.v13i3.2021.132-141.

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Introduction: Environmental management systems (EMS) were used commonly by companies to control industrial impacts and support environmental sustainability. In achieving SDG’s indicators to focuses on improving sustainable practices in the company environment, the government faces a tough challenge in increasing ISO 14001 certified companies. This study aimed to analyze the barriers and benefits of ISO 14001 certification in the industrial sector and recommend encouraging companies to carry out ISO 14001 certification. Methods: This study used a descriptive analysis method to identify the perception of ISO 14001 certified company practitioners of the certification barriers and benefits. Through survey questionnaires, data collection was conducted to 83 ISO14001 certified companies with a response rate of 49.4%. Results and Discussion: The main barrier in performing the ISO 14001 was the cost of implementation and certification. The highest benefit of ISO 14001 certification was to improve the company's image. A total of 83% of the companies believed that the benefits of ISO 14001 certification outweighed the costs incurred. Conclusion: The companies did not have significant barriers in conducting the ISO 14001 certification and classified into the medium category. The ISO 14001 certification could provide environmental sustainability, social, market, and economic benefits. The government needs to support the interest of companies in conducting the ISO 14001 implementation by overcoming the obstacles and maximizing the benefits.

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Wang, Xiao, and Dirk Burghardt. "Building-network: Concept, generation method and centrality analysis." Proceedings of the ICA 2 (July 10, 2019): 1–8. http://dx.doi.org/10.5194/ica-proc-2-141-2019.

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<p><strong>Abstract.</strong> Buildings are among the most important features of cities. In the suburban or rural regions, buildings are normally constructed along the roads, which forms the smooth and consistent patterns so that the building arrangements also can be described with network models. In previous studies, network theory has achieved good performance in cartography and GIS. In this paper, a study of a building-network is proposed, including the concepts, generation methods and centrality analysis. Firstly, with the constraint Delaunay triangulation and the refinement strategy by facing ratio, the building-network is generated by considering the buildings and the proximal segments as the nodes and segments of the network, respectively. Then, centrality analysis is applied on the building-network, aiming to reveal the crucial relationships among buildings, which is useful for understanding the structural properties of the complex network. Four different centrality measures, i.e. degree, closeness, betweenness, and eigenvector centrality, are calculated based on the building-networks. The buildings show different distribution effects and patterns under the four centrality measures. From the results, the degree centrality reveals the local centre of the region; closeness and eigenvector centrality have the ability to cluster buildings into different groups; while betweenness centrality can detect the linear patterns. Therefore, using network theory to analyse buildings can reveal some inner relationships of buildings and has great potential in the application of building pattern detection, classification, clustering and further generalization.</p>

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Uwidia, Ita E. "Treatment of Kitchen Wastewater using Aerobic Biological Method and Sand-Bed Filtration." International Journal of Chemistry 12, no. 2 (September 8, 2020): 12. http://dx.doi.org/10.5539/ijc.v12n2p12.

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Increased global demand for freshwater far exceeds the quantity available for human sustenance. Wastewater recycling processes are necessary and capable of producing large volumes of water for reuse. In this study, raw kitchen wastewater was analyzed for physicochemical and microbiological characteristics before and after biological treatment and sand-bed filtration. The results revealed a reduction in the mean values of the following parameters: turbidity (from 6.9 to 6 NTU, a 13.04% reduction), conductivity (365 to 311 µS/cm, a 14.79% reduction), total dissolved solids (141 to 121 mg/L, a 14.18% reduction), total suspended solids (5 to 2 mg/L, a 60% reduction), biochemical oxygen demand (5.5 to 1.82 mg/L, a 66.9% reduction), chemical oxygen demand (36 to 32 mg/L, an 11.11% reduction), and total bacterial count (286 x 10-4 to 16 x 10-4 CFU, a 94.41% reduction). The kitchen wastewater was suitable for non-potable reuse. Biological treatment and sand-bed filtration rendered the kitchen wastewater useful for secondary applications such as irrigation.

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LINDEMANN, SAMANTHA, MATTHEW KMET, RAVINDER REDDY, and STEFFEN UHLIG. "Matrix-Specific Method Validation of an Automated Most-Probable-Number System for Use in Measuring Bacteriological Quality of Grade “A” Milk Products." Journal of Food Protection 79, no. 11 (November 1, 2016): 1911–18. http://dx.doi.org/10.4315/0362-028x.jfp-16-141.

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ABSTRACTThe U.S. Food and Drug Administration (FDA) oversees a long-standing cooperative federal and state milk sanitation program that uses the grade “A” Pasteurized Milk Ordinance standards to maintain the safety of grade “A” milk sold in the United States. The Pasteurized Milk Ordinance requires that grade “A” milk samples be tested using validated total aerobic bacterial and coliform count methods. The objective of this project was to conduct an interlaboratory method validation study to compare performance of a film plate method with an automated most-probable-number method for total aerobic bacterial and coliform counts, using statistical approaches from international data standards. The matrix-specific validation study was administered concurrently with the FDA's annual milk proficiency test to compare method performance in five milk types. Eighteen analysts from nine laboratories analyzed test portions from 12 samples in triplicate. Statistics, including mean bias and matrix standard deviation, were calculated. Sample-specific bias of the alternative method for total aerobic count suggests that there are no large deviations within the population of samples considered. Based on analysis of 648 data points, mean bias of the alternative method across milk samples for total aerobic count was 0.013 log CFU/ml and the confidence interval for mean deviation was −0.066 to 0.009 log CFU/ml. These results indicate that the mean difference between the selected methods is small and not statistically significant. Matrix standard deviation was 0.077 log CFU/ml, showing that there is a low risk for large sample-specific bias based on milk matrix. Mean bias of the alternative method was −0.160 log CFU/ml for coliform count data. The 95% confidence interval was −0.210 to −0.100 log CFU/ml, indicating that mean deviation is significantly different from zero. The standard deviation of the sample-specific bias for coliform data was 0.033 log CFU/ml, indicating no significant effect of milk type.

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Satrio, Teguh, and M. Robiul Fuadi. "The Risk Assessment of Clinical Pathology Laboratory in Universitas Airlangga Hospital Surabaya." Indonesian Journal of Occupational Safety and Health 9, no. 2 (August 19, 2020): 131. http://dx.doi.org/10.20473/ijosh.v9i2.2020.131-141.

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Introduction: Laboratory is a place for the analysis of a material that aims for research, education, quality testing and diagnose diseases. Working in the laboratory will always be faced with a variety of risk and sources of danger that can cause workplace accidents. The purpose of this study was to determine the hazards, hazard identification, risk identification, risk assessments, risk control, and residual risk in working at the clinical pathology laboratory. Method: Data collection method used was observational and descriptive research. When viewed from the time of the study, this study was cross sectional. The data used were primary and secondary data, in which the primary data were obtained by direct observation on site, while secondary data were obtained from SOPs available in the laboratory. Results: The results of the study can identify as many as 9 hazards. In the assessment of risk, it obtained the highest level of danger which was high risk. The level of risk was as high as 3 hazards including needling, splattered patient samples, and reagents or hazardous substances. Conclusion: The conclusion from the study is that this laboratory is classified as dangerous because it finds some of the highest risk levels, which is high risk. The existing risk control in this laboratory is quite complete and good, but it needs to be an improvement in terms of compliance in wearing Personal Protective Equipment (PPE) to minimize the risk.Keywords: clinical pathology laboratory, hazard identification, risk assessment

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Nasrullah, Aan. "MARKETING PERFORMANCE DETERMINANT OF HALAL PRODUCTS IN INDONESIA." HUNAFA: Jurnal Studia Islamika 16, no. 1 (September 3, 2019): 111–41. http://dx.doi.org/10.24239/jsi.v16i1.538.111-141.

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Abstract. The aims of this study are (1) To find out and analyze the factors that contribute greatly to the performance of marketing halal products, (2) To find out and analyze marketing performance factors that have the most dominant multiplier effect on the development of halal products in Indonesia. The data analysis technique used is Confirmatory Factor Analysis (CFA). The results of this study show that based on the results of the analysis with the CFA method there are five independent variables that have a contribution to the dependent variable. First the Product Competition Level variable with a correlation value of 0.920. Second, HR Marketing Performance variable which has a correlation value of 0.895. Third, Infrastructure variables with a correlation value of 0.873. Fourth, Amount of Distribution Cost with a correlation value of 0.741, as well as the five variables X3, namely Market Competition Level variables with a correlation value of 0.572. Correlation value-of the five variables shows a strong relationship between the five independent variables with the performance variable of marketing halal products. From a number of marketing performance factors formed through literature review and after analysis using the CFA method, product competition variables are variables that have a correlation value of 0.920. The matrix component value shows a strong correlation between product competition variables and the performance of halal product marketing. The correlation value is greater than the other four independent variables. Keywords: marketing performance, halal products, confirmatory factor analysis

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Arfamaini, Revi. "PERAN PENGAWAS DALAM MENERAPKAN PENGENDALIAN INTERN PEMBERIAN KREDIT PADA GABUNGAN KOPERASI PEGAWAI REPUBLIK INDONESIA JAWA TIMUR." Jurnal Ekonomi dan Bisnis Airlangga 29, no. 2 (September 30, 2020): 132. http://dx.doi.org/10.20473/jeba.v29i22019.132-141.

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Introduction: Implementation of internal control is one of theorganization's efforts must be made to improve the effectiveness of its operations. How to implement internal control is to implement an integrated framework published by COSO in 1992. Internal control not only on the organization, credit unions must implement internal control. This is because credit unions have an activity in the form of lending on credit to members. Lending on credit can lead to an unsuccessfuluncollectible receivables that have threats that can cause harm. Threatsinclude non-performing loans, human error and fraud. Internal controls will be run in accordance with the plan, if the controller cooperativesperform functions as an internal auditor to evaluate and provide recommendations on the implementation of internal controlimprovements that have been running on credit unions. Thus, the purpose of this study was to determine the role of the controller as afunction of the internal auditor in the application of internal control ofcredit on GKPRI JATIM.Methods: The method used is the method of participation observationto determine the application of internal control GKPRI JATIM. Furthermore, assessment of internal control so as to know GKPRI JATIMcontroller role as internal auditor function components based control framework published by COSO in 1992.Results: As a result, controllers should consider several things that madecooperative organizational structure, procedures and policies areimplemented cooperative lending, risk mapping and assessment of allcredit control activities undertaken management to be able to detectproblems that emerge early on.Conclusion and suggestion: Thus, internal control can be run effectively.

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