Journal articles on the topic 'Methionine supplementation'
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Kalbande, V. H., K. Ravikanth, S. Maini, and D. S. Rekhe. "Methionine Supplementation Options in Poultry." International Journal of Poultry Science 8, no. 6 (May 15, 2009): 588–91. http://dx.doi.org/10.3923/ijps.2009.588.591.
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Tripodi, Farida, Andrea Castoldi, Raffaele Nicastro, Veronica Reghellin, Linda Lombardi, Cristina Airoldi, Ermelinda Falletta, et al. "Methionine supplementation stimulates mitochondrial respiration." Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1865, no. 12 (December 2018): 1901–13. http://dx.doi.org/10.1016/j.bbamcr.2018.09.007.
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Ratriyanto, Adi. "Pola Produksi Telur Puyuh yang Diberi Ransum Disuplementasi Betain dan Metionin." Caraka Tani: Journal of Sustainable Agriculture 33, no. 1 (March 29, 2018): 1. http://dx.doi.org/10.20961/carakatani.v33i1.19354.
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This study investigated the egg production pattern at the onset of lay until peak production in Japanese quails (Coturnix coturnix japonica) fed diet supplemented with betaine and methionine. Three hundred laying quails were randomly allocated to three groups of supplementations with 5 replicates of 20 quails. The three groups of treatments included basal diet (Control) or basal diet supplemented with 1.2 g betaine and 1.2 g methionine per kg diet. During onset of lay until 50% egg production (6 to 9 weeks of age), all groups were fed a basal diet, while the supplements were applied after 9 weeks. The egg production data were analyzed with analysis of variance and followed by Duncan’s test for significant results. During week 6 to 9, all groups of quails generated similar egg production since they received the basal diet. On the later phase, supplementation of betaine and methionine enhanced average weekly egg production (P<0.05) starting from week 10 compared with those without supplementation. The egg production curves indicated that production rapidly reached 60% soon after the onset of lay. Then, egg production rose sharply and reached the peaks of more than 80%, indicating good egg production patterns. Supplementation of betaine and methionine enhanced the average overall and peak production (P<0.01) without affecting the age at peak production. In addition, there were positive correlations between average of overall production and peak production of all treatments. It can be concluded that supplementation of betaine and methionine enhanced egg production in quails.
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Hu, Yili, Xiaocui Chai, Jun Men, Shen Rao, Xin Cong, Shuiyuan Cheng, and Zhixian Qiao. "Does Methionine Status Influence the Outcome of Selenomethinione Supplementation? A Comparative Study of Metabolic and Selenium Levels in HepG2 Cells." Nutrients 14, no. 18 (September 8, 2022): 3705. http://dx.doi.org/10.3390/nu14183705.
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Methionine restriction and selenium supplementation are recommended because of their health benefits. As a major nutrient form in selenium supplementation, selenomethionine shares a similar biological process to its analog methionine. However, the outcome of selenomethionine supplementation under different methionine statuses and the interplay between these two nutrients remain unclear. Therefore, this study explored the metabolic effects and selenium utilization in HepG2 cells supplemented with selenomethionine under deprived, adequate, and abundant methionine supply conditions by using nuclear magnetic resonance-based metabolomic and molecular biological approaches. Results revealed that selenomethionine promoted the proliferation of HepG2 cells, the transcription of selenoproteins, and the production of most amino acids while decreasing the levels of creatine, aspartate, and nucleoside diphosphate sugar regardless of methionine supply. Selenomethionine substantially disturbed the tricarboxylic acid cycle and choline metabolism in cells under a methionine shortage. With increasing methionine supply, the metabolic disturbance was alleviated, except for changes in lactate, glycine, citrate, and hypoxanthine. The markable selenium accumulation and choline decrease in the cells under methionine shortage imply the potential risk of selenomethionine supplementation. This work revealed the biological effects of selenomethionine under different methionine supply conditions. This study may serve as a guide for controlling methionine and selenomethionine levels in dietary intake.
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Meng, Bin, Weina Gao, Jingyu Wei, Lingling Pu, Zhenchuang Tang, and Changjiang Guo. "Quercetin Increases Hepatic Homocysteine Remethylation and Transsulfuration in Rats Fed a Methionine-Enriched Diet." BioMed Research International 2015 (2015): 1–8. http://dx.doi.org/10.1155/2015/815210.
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This study was aimed at investigating the effects of quercetin on mRNA expression and activity of critical enzymes in homocysteine metabolism in rats fed a methionine-enriched diet. Rats were fed for 6 weeks the following diets, that is, control, 0.5% quercetin, 1.0% methionine, and 1.0% methionine plus 0.5% quercetin diets. Serum homocysteine was significantly increased after methionine treatment and decreased after the addition of quercetin. The mRNA expression of methionine synthase was significantly increased after methionine or methionine plus quercetin supplementation, while its enzymatic activity was significantly increased after methionine plus quercetin supplementation. The mRNA expression and enzymatic activity of cystathionineβ-synthase and cystathionineγ-lyase were upregulated after quercetin, methionine, or quercetin plus methionine treatment and a more significant increase was observed for hepatic cystathionineβ-synthase in the methionine plus quercetin treated rats, suggesting an interaction between methionine and quercetin. Meanwhile, hepatic ratio of S-adenosylmethionine to S-adenosylhomocysteine was significantly decreased in response to methionine supplementation and normalized after the addition of quercetin. It is concluded that quercetin reduces serum homocysteine by increasing remethylation and transsulfuration of homocysteine in rats exposed to a methionine-enriched diet.
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Del Vesco, Ana Paula, Eliane Gasparino, Daiane de Oliveira Grieser, Vittor Zancanela, Maria Amélia Menck Soares, and Adhemar Rodrigues de Oliveira Neto. "Effects of methionine supplementation on the expression of oxidative stress-related genes in acute heat stress-exposed broilers." British Journal of Nutrition 113, no. 4 (January 23, 2015): 549–59. http://dx.doi.org/10.1017/s0007114514003535.
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The aim of the present study was to evaluate the effects of heat stress (HS) and methionine supplementation on the markers of stress and on the gene expression levels of uncoupling proteins (UCP), betaine–homocysteine methyltransferase (BHMT), cystathionine β-synthase (CBS), glutathione synthetase (GSS) and glutathione peroxidase 7 (GPx7). Broilers from 1 to 21 d and from 22 to 42 d of age were divided into three treatment groups related to methionine supplementation: without methionine supplementation (MD); recommended level of methionine supplementation (DL1); excess methionine supplementation (DL2). The broilers were either kept at a comfortable thermal temperature or exposed to HS (38°C for 24 h). During the starter period, we observed the effects of the interaction between diet and environment on the gene expression levels of UCP, BHMT and GSS. Higher gene expression levels of UCP and BHMT were observed in broilers that were maintained at thermal comfort conditions and received the MD diet. HS broilers fed the DL1 and DL2 diets had the highest expression level of GSS. The expression levels of the CBS and GPx7 genes were influenced by both the environment and methionine supplementation. During the grower period, the gene expression levels of BHMT, CBS, GSS and GPx7 were affected by the diet × environment interaction. A higher expression level of BHMT was observed in broilers maintained at thermal comfort conditions and on the MD diet. HS induced higher expression levels of CBS, GSS and GPx7 in broilers that received the DL1 and DL2 diets. The present results suggest that under HS conditions, methionine supplementation could mitigate the effects of stress, since methionine contributed to the increased expression levels of genes related to antioxidant activity.
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Brouwerv, Ingeborg A., Marijke van Dusseldorp, Marinus Duran, Chris M. G. Thomas, Joseph G. A. J. Hautvast, Tom K. A. B. Eskes, and Régine P. M. Steegers-Theunissen. "Low-dose folic acid supplementation does not influence plasma methionine concentrations in young non-pregnant women." British Journal of Nutrition 82, no. 2 (August 1999): 85–89. http://dx.doi.org/10.1017/s0007114599001221.
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An elevated plasma total homocysteine (tHcy) concentration is a risk factor for cardiovascular disease and for having offspring with a neural-tube defect. Folate is a methyl donor in the remethylation of homocysteine into methionine. Although folic acid supplementation decreases tHcy concentrations, effects of folic acid supplementation on plasma methionine concentrations are unclear. There is also concern that folic acid supplementation negatively affects vitamin B12 status. We studied effects of low-dose folic acid supplementation on methionine and vitamin B12 concentrations in plasma. We also investigated whether baseline plasma methionine and tHcy concentrations correlated with the baseline folate and vitamin B12 status. For a period of 4 weeks, 144 young women received either 500 μg folic acid each day, or 500 μg folic acid and placebo tablets on alternate days, or a placebo tablet each day. Plasma methionine, tHcy and plasma vitamin B12 concentrations were measured at start and end of the intervention period. Folic acid supplementation had no effect on plasma methionine or plasma vitamin B12 concentrations although it significantly decreased tHcy concentrations. Plasma methionine concentrations showed no correlation with either tHcy concentrations (Spearman rs - 0·01, P = 0·89), or any of the blood vitamin variables at baseline. Baseline tHcy concentrations showed a slight inverse correlation with baseline concentrations of plasma vitamin B12 (rs - 0·25, P < 0·001), plasma folate (rs - 0·24, P < 0·01) and erythrocyte folate (rs - 0·19, P < 0·05). In conclusion, low-dose folic acid supplementation did not influence plasma methionine or plasma vitamin B12 concentrations. Furthermore, no correlation between plasma methionine concentrations and the blood folate and vitamin B12 status was shown.
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Widiyastuti, Titin, and Tri Rahardjo Sutardi. "Amino Acid and Mineral Supplementation in Fermentation Process of Concentrate Protein of Jatropha Seed Cake (Jatropha curcas L.)." ANIMAL PRODUCTION 18, no. 3 (October 10, 2016): 141. http://dx.doi.org/10.20884/1.anprod.2016.18.3.574.
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The purpose of this study is to assess the optimization of fermentation process by adding a minerals and amino acids so that the potential of protein of Concentrate Protein-Jatropha seed cake (CP-JSC) can be optimally used as a substitute for soybean meal. The method used was completely randomized design. The treatment consisted of F1: Fermentation CP-BBJ + methionine-lysine (0.25%: 0.25%), F2: Fermentation CP-JSC + methionine-lysine (0.5%: 0.5%), F3: F1 + 0.45% Dicalsium Phosphate, F4: F2 + 0.45% Dicalsium Phosphate. Each treatment was repeated four times, When treatment significantly continued by Least Significant Difference (LSD), variables observed are the levels of antinutrients (phorbolester, antitrypsin), the levels of nutrients (fat, protein, crude fiber, Ca, P and gross energy) and amino acid. Results of analysis of variance showed that the addition of amino acids and minerals Ca, P in the fermentation process was highly significant effect on the levels of crude fiber and phosphorus (P <0.01) and significantly affected the gross energy content of CP-JSC post-fermentation (P <0.05). Dry matter, crude protein, crude lipid and calcium are not affected by supplementation of methionine and lysine as well as calcium and phosphorus. Supplementation of methionine and lysine in the fermentation substrate showed good levels of essential amino acids and non essential higher than previous studies although not statistically significant (P> 0.05). While the levels obtained phorbolester range of 0.055% - 0.08%. It was concluded that the optimization of fermentation can be done without adding the amino acid supplementation of minerals calcium and phosphorus. Supplementation significantly affect a significant increase or decrease in some nutrients (crude fiber, gross energy, phosphor) and capable of suppressing a decrease in amino acids. Supplementation of amino acids Lysine and Methionin 0.05% is the best treatment.
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Huisman, J., E. J. van Weerden, P. van Leeuwen, and F. Koch. "Effect of methionine compounds on rumen activity of cows." Netherlands Journal of Agricultural Science 36, no. 2 (May 1, 1988): 111–17. http://dx.doi.org/10.18174/njas.v36i2.16684.
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Rate of degradation of hay was studied, using the nylon-bag technique, in 2 cows given a basal diet without (controls) or with a liquid methionine supplement (LiquimethReg.) or a protected methionine supplement (MepronReg.) providing methionine 25 g daily. After 24 and 48 h incubation methionine supplementation significantly increased degradation of DM, organic matter and cell wall constituents compared with controls. Degradability of crude protein (CP) was significantly increased by methionine supplementation, and degradability was affected by type of supplement with degradability of CP greater with the liquid methionine supplement than with the protected methionine supplement. (Abstract retrieved from CAB Abstracts by CABI’s permission)
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Lambert, B. D., C. A. Löest, and Evan C. Titgemeyer. "Effect of methionine supplementation on methionine metabolism in growing cattle." Kansas Agricultural Experiment Station Research Reports, no. 1 (January 1, 2002): 17–19. http://dx.doi.org/10.4148/2378-5977.1696.
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Yu, Manrong, Hui Chen, Pan Liu, Mei Yang, Leqin Zou, and Dingfu Xiao. "Antioxidant Function and Metabolomics Study in Mice after Dietary Supplementation with Methionine." BioMed Research International 2020 (October 20, 2020): 1–6. http://dx.doi.org/10.1155/2020/9494528.
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The antioxidant function and metabolic profiles in mice after dietary supplementation with methionine were investigated. The results showed that methionine supplementation enhanced liver GSH-Px activity and upregulated Gpx1 expression in the liver and SOD1 and Gpx4 expressions in the jejunum. Nrf2/Keap1 is involved in oxidative stress, and the western blotting data exhibited that dietary methionine markedly increased Keap1 abundance, while failed to influence the Nrf2 signal. Metabolomics investigation showed that methionine administration increased 2-hydroxypyridine, salicin, and asparagine and reduced D-Talose, maltose, aminoisobutyric acid, and inosine 5’-monophosphate in the liver, which are widely reported to involve in oxidative stress, lipid metabolism, and nucleotides generation. In conclusion, our study provides insights into antioxidant function and liver metabolic profiles in response to dietary supplementation with methionine.
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Yesmin, S., ME Uddin, R. Chacrabati, and M. Al-Mamun. "Effect of methionine supplementation on the growth performance of rabbit." Bangladesh Journal of Animal Science 42, no. 1 (July 22, 2013): 40–43. http://dx.doi.org/10.3329/bjas.v42i1.15777.
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The present study was conducted to evaluate the effect of different levels of methionine supplementation on feed intake, nutrient digestibility and growth performance of growing rabbit. Sixteen weaned crossbred New Zealand White (NZW) growing rabbits (30-35 d) were distributed into four treatment groups having four replications in each group using a Completely Randomized Design (CRD). Basal diet composed of green grass (dhal grass) and concentrate mixture which was offered ad libitum basis for 56 days period. Four levels of methionine such as 0.0% (control), 0.15%, 0.25%, and 0.35% were supplemented randomly to rabbits. Results showed that supplementation of methionine did not affect green grass intake. Cumulative as well as daily concentrate and DM intake were significantly (p<0.05) higher for all methionine groups than control group. Final body weight gain as well as daily, weekly and cumulative body weight gains were improved significantly with increasing level of methionine. It was found that methionine had significant (p<0.01) effect on digestibility of DM, CP, NFE and EE but CF digestibility did not differ significantly. Digestibility was improved with increasing the level of methionine. Feed conversion ratio also decreased significantly with methionine supplementation, and 0.25% methionine group showed the best performance among the four treatments. DOI: http://dx.doi.org/10.3329/bjas.v42i1.15777 Bang. J. Anim. Sci. 2013. 42 (1): 40 43
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CHILLIARD, YVES, and MICHEL DOREAU. "Influence of supplementary fish oil and rumen-protected methionine on milk yield and composition in dairy cows." Journal of Dairy Research 64, no. 2 (May 1997): 173–79. http://dx.doi.org/10.1017/s0022029996002105.
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The effects of a daily supplement of 300 ml fish oil and 20 g rumen-protected methionine, alone or in combination, were investigated in mid-lactation cows receiving a maize silage-based diet ad lib. Fish oil significantly decreased feed intake, increased milk yield, decreased protein and casein concentrations, and especially fat concentration (by 13·1 g/kg) and output. Fat concentration decreased more in primiparous than in multiparous cows. Methionine supplementation increased protein and casein concentrations and outputs. No significant interaction between oil and methionine supplementation was found on milk composition. Treatments did not modify live weight or body condition changes, or lactose and non-casein nitrogenous compounds in milk. Oil plus methionine supplementation made it possible to decrease milk fat content without changing protein content.
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Agunbiade, J. A., and A. B. Odufuwa. "COMPARATIVE UTILIZATION OF COOKED SOYBEANS AND FISHMEAL-GROUNDNUT CAKE MIXTURE IN DIETS." Nigerian Journal of Animal Production 22, no. 2 (January 10, 2021): 145–49. http://dx.doi.org/10.51791/njap.v22i2.2431.
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A 4 week experiment involving 45 growing albino rats was carried out to assess the nutritional utilization of cooked full-fat soybean (CFFS) as a major source of dietary protein relative to that of the protein concentrates fishmeal-groundnut cake mixture and the response to supplementation of the cooked soybeans with 2g DL-methionine/kg diet and/or 2g L-lysine/kg diet. Feed intake (FI), average daily weight gain (DLG),. feed conversion ratio (FCR), protein efficiency ratio (PER) and feed cost (FC) per unit live-weight gain, (FC/WG) were measured, No significant effect of the various dietary treatments was observed on PER and FCIWG. However, the incorporation of CFFS either alone or in conjunction with either of both lysine and methionine significantly improved Fl and FCR (P< 0.05) and also DLG (P< 0.01) over the control fish-groundnut meal ration. Supplementation of CFFS with lysine either alone or along with methionine appeared unwarranted as methionine supplementation alone resulted in similar or slightly better efficiency of protein (0.247) and overall feed (0.596) utilization. From the results obtained, it appears that maize-based CFFS diet supplemented with 2 g/kg DL-methionine may be applicable for lion- ruminant feeding. However, since this level of methionine supplementation only provides for 67% of rat requirement, it is suggested that further studies with higher methionine levels be conducted.
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Nugroho, Rudy Agung, Normala Sari, Retno Aryani, Hetty Manurung, and Rudianto Rudianto. "Growth evaluation of Oreochromis niloticus fed different concentrations of choline and methionine." Aceh Journal of Animal Science 5, no. 2 (September 21, 2020): 122–28. http://dx.doi.org/10.13170/ajas.5.2.17496.
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A 12-week research was conducted to determine the effects of choline and methionine supplementation on the growth performance of Nile tilapia (Oreochromis niloticus). Three diets, P2-P4 with 0.04; 0.08; 0.12 % of choline; P6-P8 with same levels choline plus methionine (0.15 %); a diet with only 0.15 % methionine (P5); and control without any supplementation (P1) were fed to tilapia (initial weight ±21.05 g) for 12 weeks. Final weight (FW), Body Weight Gain (BWG), Average Daily Gain (ADG), Specific Growth Rate (SGR), and Protein Efficiency Ratio (PER), Feed Efficiency (FE), Feed Intake (FI), Feed Conversion Rate (FCR), Survival Rate (SR), crude protein and lipid of fish fed various levels of choline and methionine were also measured. The results showed that tilapia fed 0.04 % choline (P2) had significantly better growth parameters and feed efficiency than other groups. Meanwhile, SR of tilapia was not affected by any supplementation of choline and methionine. The tilapia fed 0.08% choline (P3) showed the highest crude protein (52.50 ± 0.98 %) in the carcass proximate but low lipid (19.03 ± 0.10 %). This finding demonstrated the benefits of choline (0.04-0.08 %) supplementation in the tilapia diet in term of growth and carcass proximate.
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Fenn, PD, and RA Leng. "Wool growth and sulfur amino acid entry rate in sheep fed roughage based diets supplemented with bentonite and sulfur amino acids." Australian Journal of Agricultural Research 40, no. 4 (1989): 889. http://dx.doi.org/10.1071/ar9890889.
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In two experiments, sheep were offered a roughage-based diet supplemented with either cysteine or bentonite as a solid, or bentonite, cysteine or methionine added to their drinking water. Supplementation with cysteine as a solid had no effect on wool growth, while supplementation via drinking water had no effect on wool growth or cysteine entry rate into the blood. Supplementation with methionine via drinking water increased the entry rate of methionine into blood by 69% (P< 0.05) as measured by a continuous infusion of [35S]-methionine. This coincided with subsequent increases in wool growth of 16% (P< 0.05) compared to sheep fed a basal diet alone. Compared with the basal diet alone, supplementation with 30 g/day bentonite as a dry powder or 60 g/day as a suspension in drinking water increased wool growth by 19 and 20% respectively. Bentonite given as sole supplement did not increase the entry rate of either cysteine or methionine into the blood of sheep. When bentonite and sulfur amino acids were complexed or mixed, wool growth was not increased above that for bentonite or the amino acid alone.
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Savino, Ronald J., Mercedes Vazquez-Anon, Katarzyna Stefańska, James N. Petitte, and Paul Mozdziak. "The Influence of L-Methionine, DL-Methionine, and a Methionine Hydroxy Analog on Proliferation and Differentiation Potential of Avian Myoblasts." Medical Journal of Cell Biology 10, no. 2 (June 1, 2022): 69–82. http://dx.doi.org/10.2478/acb-2022-0012.
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Abstract Methionine is an essential amino critical to many cell functions including the synthesis of proteins. Supplementation of methionine in vivo is typically through L-methionine, DL-methionine, or a methionine hydroxy analog (MHA). The goal of this study was to compare the function of L-methionine, DL-methionine, and an MHA as a source of methionine to myoblasts in vitro. Avian myoblasts isolated from turkey embryos were plated in media containing varying concentrations of DL-methionine (DLM; 1.125 mg/mL or 0.56 mg/mL) or methionine hydroxy analog (MHA; 1.28 mg/mL or 0.64 mg/mL) as well as a methionine deficient negative control group and an L-methionine supplemented positive control group. The results of the proliferation assay exhibited cell division in the absence of methionine which was not significantly different than the positive control group. Results from the myoblast fusion assay revealed significantly greater myotube diameter between methionine supplemented groups compared to the methionine deficient negative control. The findings of this study show an ability for avian myoblasts to proliferate in the absence of methionine, the significance of which is discussed. Additionally, findings from the fusion assay suggest that DL-methionine and MHA are potential cost-effective substitutes for methionine supplementation during terminal differentiation of avian myoblasts.
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Miousse, Isabelle R., Laura E. Ewing, Charles M. Skinner, Rupak Pathak, Sarita Garg, Kristy R. Kutanzi, Stepan Melnyk, Martin Hauer-Jensen, and Igor Koturbash. "Methionine dietary supplementation potentiates ionizing radiation-induced gastrointestinal syndrome." American Journal of Physiology-Gastrointestinal and Liver Physiology 318, no. 3 (March 1, 2020): G439—G450. http://dx.doi.org/10.1152/ajpgi.00351.2019.
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Methionine is an essential amino acid needed for a variety of processes in living organisms. Ionizing radiation depletes tissue methionine concentrations and leads to the loss of DNA methylation and decreased synthesis of glutathione. In this study, we aimed to investigate the effects of methionine dietary supplementation in CBA/CaJ mice after exposure to doses ranging from 3 to 8.5 Gy of 137Cs of total body irradiation. We report that mice fed a methionine-supplemented diet (MSD; 19.5 vs. 6.5 mg/kg in a methionine-adequate diet, MAD) developed acute radiation toxicity at doses as low as 3 Gy. Partial body irradiation performed with hindlimb shielding resulted in a 50% mortality rate in MSD-fed mice exposed to 8.5 Gy, suggesting prevalence of radiation-induced gastrointestinal syndrome in the development of acute radiation toxicity. Analysis of the intestinal microbiome demonstrated shifts in the gut ecology, observed along with the development of leaky gut syndrome and bacterial translocation into the liver. Normal gut physiology impairment was facilitated by alterations in the one-carbon metabolism pathway and was exhibited as decreases in circulating citrulline levels mirrored by decreased intestinal mucosal surface area and the number of surviving crypts. In conclusion, we demonstrate that a relevant excess of methionine dietary intake exacerbates the detrimental effects of exposure to ionizing radiation in the small intestine. NEW & NOTEWORTHY Methionine supplementation, instead of an anticipated health-promoting effect, sensitizes mice to gastrointestinal radiation syndrome. Mechanistically, excess of methionine negatively affects intestinal ecology, leading to a cascade of physiological, biochemical, and molecular alterations that impair normal gut response to a clinically relevant genotoxic stressor. These findings speak toward increasing the role of registered dietitians during cancer therapy and the necessity of a solid scientific background behind the sales of dietary supplements and claims regarding their benefits.
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Muramatsu, T., M. Kato, I. Tasaki, and J. Okumura. "Enhanced whole-body protein synthesis by methionine and arginine supplementation in protein-starved chicks." British Journal of Nutrition 55, no. 3 (May 1986): 635–41. http://dx.doi.org/10.1079/bjn19860069.
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1. The effect of supplementing with methionine alone or in combination with arginine on whole-body protein synthesis and degradation was studied in protein-starved chicks, fed on a protein-free (PF) diet, by a massive-dose injection of L-[4-3H]phenylalanine.2. Methionine or methionine and arginine (MA) supplementation reduced body-weight loss and improved N balance compared with unsupplemented controls.3. Whole-body protein synthesis was significantly increased both in terms of fractional rate and absolute amounts by methionine and MA addition, whereas the fractional degradation rate was unchanged.4. No significant difference was found between methionine and MA supplementation except for energy balance of the birds.5. It was concluded that the N-sparing effect of methionine or MA when added to a PF diet was primarily brought about by enhanced whole-body protein synthesis.
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Wiese, S. C., C. L. White, D. G. Masters, J. T. B. Milton, and R. H. Davidson. "The growth performance and carcass attributes of Merino and Poll Dorset × Merino lambs fed rumen-protected methionine (Smartamine™-M)." Australian Journal of Agricultural Research 54, no. 5 (2003): 507. http://dx.doi.org/10.1071/ar02173.
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This study investigated the effect of 5 levels of rumen-protected methionine (0, 1, 2, 3, or 5 g/head.day) on growth rate, feed conversion efficiency, carcass composition, and wool growth in lambs fed a production diet ad libitum. Merino and Poll Dorset × Merino (crossbred) wether lambs (120 of each genotype) were housed in group pens each of 6 sheep. After a 1-week adjustment period, all lambs were fed a pelleted diet of lupins, cereal grain, and hay ad libitum. Rumen-protected methionine was added to the diets in the form of Smartamine™-M. The crossbred lambs were slaughtered after 28 days on the treatment diet and the Merino lambs after 42 days.Increasing the level of methionine supplementation did not lead to an increase in growth rate, daily feed intake, feed conversion efficiency, wool growth, skin thickness, or final liveweight and condition score. The Merino lambs had a lower growth rate, thinner skin, faster rate of wool growth, and were less efficient at converting feed into liveweight gain than the crossbred lambs (P < 0.05). There was no interaction between breed and methionine treatment.Increasing the level of methionine treatment also did not improve hot carcass weight, dressing percentage, tissue depth over the rib, longissimus dorsi (LD) cross-sectional area, meat pH, or meat colour. The only beneficial effect of methionine supplementation on carcass attributes was a decline in fat depth over the deepest part of the LD as methionine level increased (P < 0.05).There were no differences in tenderness, juiciness, or flavour intensity between the breeds or as a main effect with methionine supplementation. However, there was an interaction between breed and methionine for flavour intensity, such that methionine supplementation reduced the flavour intensity of Merino meat but not that of the crossbred meat (P < 0.05).This work suggests that there are unlikely to be production gains in terms of liveweight gain, feed conversion efficiency, or wool growth in supplementing lambs with protected methionine if they are being fed a high performance diet ad libitum. However, there were 2 benefits that may have scope for further investigation. The first was a reduction in fat cover over the loin as the level of methionine supplementation increased. The second was a reduction in the intensity of flavour found in Merino lamb when the lambs were supplemented with methionine. The study also provided some useful guidelines for the differences in growth and carcass characteristics between Merino and crossbred lambs when they are finished under identical conditions to an even fat cover, and demonstrated that Merino lambs can produce carcasses of very acceptable eating quality when well finished.
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Sipőcz, P., J. Schmidt, J. Sipőcz, and Éva Cenkvári. "USE OF PROTECTED METHIONINE (MEPRON M 85) IN CATTLE." Acta Veterinaria Hungarica 47, no. 4 (November 1, 1999): 409–18. http://dx.doi.org/10.1556/avet.47.1999.4.2.
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The ruminal stability of Mepron M 85 and the effect of supplementation with Mepron M 85 on free methionine level of blood were studied in rumen-fistulated cows and rumen- and duodenum-fistulated growing bulls. In five rumen-fistulated cowsin situ69.5% and 64.6% of the methionine content of Mepron M 85 was found after ruminal incubation of 16 h and 24 h, respectively. Daily rations of the rumen-fistulated cows were supplemented with 15.0 g DL-methionine and 17.7 g Mepron M 85, which increased the free methionine level of blood from 13.64 (mol/L to 15.35 and 20.46 (mol/L, respectively, three hours after feeding. In the four rumen- and duodenum-fistulated growing bulls, supplementation with 15.0 g DL-methionine and 17.7 g Mepron M 85 increased the total methionine getting into the duodenum during 24 h from 14.99 g to 16.84 and 20.84 g, respectively. The influence of Mepron M 85 on milk production was studied in 35 pairs of Hungarian Fleckvieh × Holstein-Friesian cows. The animals were coupled on the basis of the number of finished lactations, milk production in the previous lactation, and the date of calving. Daily supplementation of 18.0 g Mepron M 85 increased daily milk production significantly (p < 0.05), by 1.24 litres. Milk fat content also increased significantly (from 3.10% to 3.19%, p < 0.05) in the experimental group. The supplementation did not influence milk protein content.
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Tripodi, Farida, Beatrice Badone, Marta Vescovi, Riccardo Milanesi, Simona Nonnis, Elisa Maffioli, Marcella Bonanomi, Daniela Gaglio, Gabriella Tedeschi, and Paola Coccetti. "Methionine Supplementation Affects Metabolism and Reduces Tumor Aggressiveness in Liver Cancer Cells." Cells 9, no. 11 (November 16, 2020): 2491. http://dx.doi.org/10.3390/cells9112491.
Full textAbstract:
Liver cancer is one of the most common cancer worldwide with a high mortality. Methionine is an essential amino acid required for normal development and cell growth, is mainly metabolized in the liver, and its role as an anti-cancer supplement is still controversial. Here, we evaluate the effects of methionine supplementation in liver cancer cells. An integrative proteomic and metabolomic analysis indicates a rewiring of the central carbon metabolism, with an upregulation of the tricarboxylic acid (TCA) cycle and mitochondrial adenosine triphosphate (ATP) production in the presence of high methionine and AMP-activated protein kinase (AMPK) inhibition. Methionine supplementation also reduces growth rate in liver cancer cells and induces the activation of both the AMPK and mTOR pathways. Interestingly, in high methionine concentration, inhibition of AMPK strongly impairs cell growth, cell migration, and colony formation, indicating the main role of AMPK in the control of liver cancer phenotypes. Therefore, regulation of methionine in the diet combined with AMPK inhibition could reduce liver cancer progression.
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Rosa-Velazquez, Milca, Juan M. Pinos-Rodriguez, and Alejandro E. Relling. "31 Effect of polyunsaturated fatty acid and methionine supplementation during late gestation on offspring growth, glucose tolerance test, and carcass characteristic in sheep." Journal of Animal Science 98, Supplement_3 (November 2, 2020): 112–13. http://dx.doi.org/10.1093/jas/skaa054.190.
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Abstract The objective of this study was to evaluate the effect of supplementing polyunsaturated fatty acids (PUFA), methionine (M) or both to ewes during late gestation on offspring growth, dry matter intake (DMI), carcass composition, and glucose and insulin metabolism (by conducting a glucose tolerance test (GTT)). Seventy-two post-weaned lambs (6 pens/treatment, 3 lambs/pen) born from ewes supplemented from gestation day 100 until lambing were used. Dam supplementation was: 1) no PUFA nor M supplementation (NFA-NM); 2) supplementation with PUFA (PUFA-NM, 1 % StrataG113); 3) supplementation with M (NFA-MET, 0.1 % rumen protected methionine, Smartamine); and 4) supplementation with PUFA and M (PUFA-MET). At weaning (60 days of age) lambs were fed a common finishing diet for 54 days, and DMI was measured daily. Lambs were weighed on weaning day (d0), d28, and d54. Twenty-four lambs (one per pen) were used for a GTT on d55. On d56, other 24 lambs were harvested for carcass characteristics measurements. Offspring data was analyzed as a 2x2x2 factorial (FA, M, and sex). Ewe supplementation affected (P < 0.1) lamb DMI. On d54 lambs born from No FA and methionine showed a greater DMI. There was a tendency for plasma glucose concentration for PUFA*MET*Time (P = 0.07) in the GGT. Lambs born from ewes supplemented with methionine had greater glucose concentration than NFA-NM born lambs, and lambs born from PUFA*MET had greater glucose concentration than the other lambs at 2m of the GGT. Dam MET supplementation decreased lamb’s insulin response (P = 0.06). Hot carcass weight (HCW) (P = 0.08) and Ribeye area (REA) (P = 0.02) were affected by treatment and sex. A tendency and a triple interaction FA*MET*Sex were observed; NFA-MET methionine had a greater HCW and a larger REA. In conclusion, lamb’s insulin response and carcass characteristics are affected by dam’s supplementation.
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Silva, Jackeline Thais, Evangelina Miqueo, Thaís Manzoni Torrezan, Nathalia Brito Rocha, Giovana Simão Slanzon, Gercino Ferreira Virginio Júnior, and Carla Maris Machado Bittar. "Supplementation of Lysine and Methionine in Milk Replacer or Starter Concentrate for Dairy Calves in Step-Up/Step-Down Feeding Program." Animals 11, no. 10 (September 29, 2021): 2854. http://dx.doi.org/10.3390/ani11102854.
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This study aimed to evaluate the performance and metabolic changes in dairy calves fed in a step-up/step-down program and supplemented with lysine and methionine in a milk replacer (MR) or starter concentrate (SC). Male Holstein calves (n = 45) were blocked and distributed in the control without supplementation (1) and with lysine and methionine supplementation in the SC to achieve an intake of 17 and 5.3 g/d, respectively (2), and in the MR to achieve the same daily intake (3). MR was fed 4 L/d until the 2nd week, 8 L/d from the 3rd to 6th week, and 4 L/d from the 7th to 8th week, when calves were weaned. The calves were followed until the 10th week of age. Feed intake was measured daily. Weight and body measurements were registered weekly, and blood samples were collected biweekly. The lysine and methionine intake during the whole period was higher when supplementation occurred via MR. There was a supplementation effect for average daily gain after weaning, and the animals supplemented in the MR had lower BW than those that were not supplemented. Supplementation in MR decreased starter intake at the 10th week and total intake (g DM/d) after weaning. Supplementation with lysine and methionine in the MR or the SC did not benefit the performance or metabolism of dairy calves in the step-up/step-down program. Further studies are needed to understand the effects of amino acid supplementation on feed intake.
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Van Der Westhuyzen, Justin, Suasn V. Van Tonder, Jayne E. Gibson, Terence A. Kilroe-smith, and Jack Metz. "Plasma amino acids and tissue methionine levels in fruit bats (Rousettus aegyptiacus) with nitrous oxide-induced vitamin B12 deficiency." British Journal of Nutrition 53, no. 3 (May 1985): 657–62. http://dx.doi.org/10.1079/bjn19850075.
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1. The effect of methylcobalamin inactivation by the gas nitrous oxide on plasma amino acid and tissue methionine levels in fruit bats (Rousettus aegyptiacus) was examined.2. Animals exposed to N2O-oxygen (1:1, v/v) for 90 min daily received a fruit diet with or without methionine or betaine supplements. Exposure and diets were continued for up to 17 weeks or until neurological impairment and muscular weakness was established.3. All the groups exposed to N2O had significantly lower liver, brain and plasma methionine concentrations except the methionine-supplemented animals which showed significantly raised levels. Plasma homocysteine, which was absent in controls, was present in all the N2O-exposed groups.4. Betaine supplementation resulted in reduced accumulation of homocysteine in plasma. However, plasma and liver methionine levels were only slightly increased compared with animals on the basal diet, and brain methionine levels were the lowest of all the groups studied.5. These results support the hypothesis that reduced methionine synthesis is an important contributor to the development of neurological impairment in this species and suggest that dietary supplementation with the methionine precursor betaine cannot replace the loss of vitamin B12-dependent methionine synthesis.
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Etuk, E. B., O. N. Abaoja, and B. O. Esonu. "Raw Pigeon seed meal in Weaner Rabbit Diet: Effect of Methionine Supplementation." Nigerian Journal of Animal Production 34, no. 2 (January 9, 2021): 241–46. http://dx.doi.org/10.51791/njap.v34i2.1195.
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Effect of graded levels of methionine supplementation on the performance of weaner rabbits fed diets containing raw pigeon pea seed meal were studied in a 42-day feeding trial. Four experimental weaner rabbit diets were formulated such that raw pigeon pea seed meal (RPSM) made up 300.0g kg-1 in each diet. Methionine was respectively supplemented at 0.0, (control diet), 2.5, 5.0 and 7.5 g kg-1 in the four weaner rabbit diets. These diets and water were offered ad libitum to 3 replicates of 2, six weeks old mixed breed rabbits. The results indicated that the daily weight gains were 2.62, 2.85, 5.23 and 4.64 for rabbits fed 0.0, 2.5, 5.0 and 7.5g kg-1 methionine supplemented diets respectively. Feed intake of rabbits fed 7.55 kg-1 methionine supplemented diets was significantly (P<0.05) lower than other treatment groups except the control. Rabbits fed 0.0 and 2.5 g kg-1 methionine supplemented diets recorded significantly (p<0.05) poorer feed conversion ratio than rabbits fed 5.0 and 7.55 kg-1 methionine supplemented diets. The feed cost per kg weight gains were ₦363.58, ₦351.49, ₦197.64, ₦204.38 for rabbits fed 0.0, 2.5, 5.0 and 7.5 g kg-1 methionine supplemented diets respectively. It would appear that weaner rabbits performed best on 300.0g kg-1 raw pigeon pea seed meal diet when supplemented with methionine at 5.0g kg-1 diet.
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Kwon, Young Hye, and Martha H. Stipanuk. "Cysteine regulates expression of cysteine dioxygenase and γ-glutamylcysteine synthetase in cultured rat hepatocytes." American Journal of Physiology-Endocrinology and Metabolism 280, no. 5 (May 1, 2001): E804—E815. http://dx.doi.org/10.1152/ajpendo.2001.280.5.e804.
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Rat hepatocytes cultured for 3 days in basal medium expressed low levels of cysteine dioxygenase (CDO) and high levels of γ-glutamylcysteine synthetase (GCS). When the medium was supplemented with 2 mmol/l methionine or cysteine, CDO activity and CDO protein increased by >10-fold and CDO mRNA increased by 1.5- or 3.2-fold. In contrast, GCS activity decreased to 51 or 29% of basal, GCS heavy subunit (GCS-HS) protein decreased to 89 or 58% of basal, and GCS mRNA decreased to 79 or 37% of basal for methionine or cysteine supplementation, respectively. Supplementation with cysteine consistently yielded responses of greater magnitude than did supplementation with an equimolar amount of methionine. Addition of propargylglycine to inhibit cystathionine γ-lyase activity and, hence, cysteine formation from methionine prevented the effects of methionine, but not those of cysteine, on CDO and GCS expression. Addition of buthionine sulfoximine to inhibit GCS, and thus block glutathione synthesis from cysteine, did not alter the ability of methionine or cysteine to increase CDO. GSH concentration was not correlated with changes in either CDO or GCS-HS expression. The effectiveness of cysteine was equivalent to or greater than that of its precursors ( S-adenosylmethionine, cystathionine, homocysteine) or metabolites (taurine, sulfate). Taken together, these results suggest that cysteine itself is an important cellular signal for upregulation of CDO and downregulation of GCS.
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Barry, T. N., and T. R. Manley. "Responses to oral methionine supplementation in sheep fed on kale (Brassica oleracea) diets containing S-methyl-L-cysteine sulphoxide." British Journal of Nutrition 54, no. 3 (November 1985): 753–61. http://dx.doi.org/10.1079/bjn19850161.
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1. Responses to twice-weekly oral supplementation with 4.0 g methionine were measured with lambs (27 kg) grazing kale (Brassica oleracea) for 10 weeks (Expt 1). In a second experiment with sheep fed on kale at hourly intervals, rumen fractional outflow rates of CrEDTA and ruthenium Tris(1, 10 phenanthroline) markers were measured from the rates of decline in their concentrations. Rumen turnover of S-methyl-L-cysteine sulphoxide (SMCO) and of carbohydrate (CHO) constituents were also measured. The kale fed contained 11.4 g SMCO/kg dry matter and the ratio, readily-fermentable: structural CHO was high at 2.9.2. Severe haemolytic anaemia, associated with low live-weight gain (LWG), occurred in the lambs during the initial 5 weeks of grazing, due to rumen fermentation of SMCO. Oral methionine supplementation raised plasma concentrations of methionine and cysteine, increased wool growth rate, and increased LWG during the initial 5 weeks. Methionine supplementation also increased rumen pool and plasma SMCO concentrations, suggesting reduced rumen SMCO fermentation.3. In Expt 2, rumen degradation rate of SMCO (1.2/h) was calculated to be twice as fast as that of the most rapidly fermented dietary CHO constituents and eight times faster than the rate of water outflow (0.16/h), thus explaining its virtually complete rumen degradation and toxicity in brassica diets. It was estimated that 1.2 g of each 4.0 g methionine administered would have escaped rumen degradation, due to the high rate of water outflow from the rumen.4. Disappearance rates of CHO constituents from the rumen were as predicted for normal ruminant diets, showing that rumen metabolism of SMCO did not have a depressive effect. Hemicellulose disappeared at a slower rate than the other CHO, suggesting that hemicellulose digestion may be rate-limiting for cell-wall digestion.5. It is suggested that the responses to oral methionine supplementation can be explained through methionine reducing rumen SMCO degradation, and through a significant portion of the methionine escaping rumen degradation.
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Ginting, Simon P., Antonius ., and Kiston Simanihuruk. "Supplementation of inorganic and organic zinc mixtures in feed of Boerka goats fed by oil palm fronds." Jurnal Ilmu Ternak dan Veteriner 22, no. 2 (March 1, 2018): 51. http://dx.doi.org/10.14334/jitv.v22i2.1798.
Full textAbstract:
Zinc is an element of many enzymes and hormones having very important physiological functions in the body so that it influences the production and reproduction of animals including the activity of the rumen microflora in degrading fiber in a diet. The aim of this study was to improve the performances of goats offered oil palm fronds based diets through the supplementation of 35 ppm of Zn in the form of inorganic (ZnO) and organic zinc (Zn-methionine). Thirty mature male crossing Boer x Kacang (Boerka) goats were divided into five groups and randomly allocated to one of the five feed treatments as follows: P1: complete feed based on the palm oil fronds (Control), P2: P1 + 35 ppm Zn (100% Zn0), P3: P1 + 35 ppm Zn (75% Zn0 + 25% Zn-methionine), P4: P1 + 35 ppm Zn (50% Zn0 + 50% Zn-methionine), P5: P1 + 35 ppm Zn (25% Zn0 + 75% Zn-methionine). The experiment was conducted in a Completely Randomized Design of six replications. Increasing the proportion of Zn methionine in the mixtures elevated feed consumption, and the highest feed intake was observed in goats received 75% Zn-methionine/25% ZnO. Daily body weight gains was only affected (P<0.05) by the 75% Zn-methionine/25% ZnO supplement. The concentration of Zn in the blood increased significantly (P<0.05) when Zn methionine was added and it increased steadily as the proportion of Zn methionine greater in the mixtures, but the VFA compositions of the rumen were not affected (P>0.05) by Zn supplementation. It is concluded that the performances of goat fed complete diets based on the oil palm fronds could be improved by supplementation of inorganic and organic Zn mixture.
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Stephenson, RGA, GR Suter, and CJ Howitt. "Wool growth responses to DL-methionine administration and factors affecting the value of supplementation." Australian Journal of Experimental Agriculture 31, no. 4 (1991): 471. http://dx.doi.org/10.1071/ea9910471.
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Wool growth responses to methionine administration were assessed using Peppin Merino wethers on various planes of nutrition. In experiment 1, sheep were fed 700 g/day of lucerne pellets to maintain liveweight. DL-methionine (5.8 g) administered subcutaneously resulted in 0.29 mg/cm2.day (36%) more wool (P<0.01) and a 2.5 �m (15.2%) increase in fibre diameter (P<0.05). In experiment 2, two levels (700 and 1400 g/day) of lucerne pellets were offered, with and without methionine administered subcutaneously (5.8 g). Main treatment analysis indicated a significant (P<0.01) effect of nutrition on wool growth and fibre diameter, with no effect of methionine, except for a reduction (P<0.05) in liveweight gain. Methionine administration was associated with a non-significant increase (39%) in wool growth for the 700 g diet and no change for the 1400 g diet. In experiment 3, two levels (1.75 and 2.5 g/day) of methionine were infused (4 h daily) when sheep were fed a submaintenance ration of Rhodes grass chaff, supplemented with nitrogen and sulfur. Significant (P<0.05) wool growth responses at both levels of methionine infusion were similar, with absolute (0.21 and 0.23 mg/cm2.day) and percentage (52 and 58%) increases for 1.75 and 2.5 g/day methionine treatments, respectively. Methionine administration only increased wool production at nutritional levels of liveweight maintenance or below when wool growth values for control treatments were 0.63,0.59 and 0.40 mg/cm2.day for experiments 1, 2 and 3. Fibre diameters of sheep in untreated groups were 16.4 and 18.8 pm for experiments 1 and 2. These wool growth and fibre diameter values are 25-40% below what might be expected for this strain of Merino. The values of positive correlations between wool growth and fibre diameter were increased with increased nutrition and the administration of methionine in experiment 2. This result and the nil response at high basal wool growth, together with the low potential net return on the cost of methionine, suggest that the use of methionine could be unprofitable.
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Ritz, M., A. Gonzalez, A. S. Fries, T. Scheu, N. Blad-Stahl, F. Kotarski, G. Schuler, C. Koch, and C. Wrenzycki. "38 Developmental competence of bovine cumulus–oocyte complexes collected from cows fed rumen-protected methionine and lysine." Reproduction, Fertility and Development 33, no. 2 (2021): 126. http://dx.doi.org/10.1071/rdv33n2ab38.
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Supplementation of rumen-protected amino acids (RPAA) has proven to be an effective tool to supply limiting AA in dairy diets. Methionine and lysine are the two most limiting AA for lactating dairy cows. Recently, it has been shown that methionine supplementation seems to affect pre-implantation embryos collected from superovulated cows enhancing their developmental competence because there is strong evidence that endogenous lipid reserves serve as an energy substrate (Acosta et al. 2016 Theriogenology 85, 1669–1679). Moreover, higher concentrations of methionine were determined in the follicular fluid of the first dominant follicle postpartum in cows supplemented with rumen-protected methionine and rumen-protected choline from 21 days before calving to 30 days postpartum and it was assumed that higher methionine concentrations in the follicular fluid could affect oocyte quality (Acosta et al. 2017 Theriogenology 96, 1–9). There is no information available so far regarding the effect of a combined methionine and lysine supplementation (each rumen-protected) on oocyte quality. Therefore, the objective of this study was to evaluate the effect of a combined methionine and lysine supplementation during early to mid-lactation on the developmental competence of oocytes collected from lactating dairy cows (days 0 to 100 p.p.). Thirty pregnant multiparous German Holstein dairy cows were grouped 3 weeks before their expected calving date, receiving identical diets. After calving, they were randomly allocated to 2 groups fed a total mixed ration supplemented with (N=14 cows; RPAA) or without (N=16 cows; CON) LysiGEMTM (encapsulated lysine; Kemin Industries) and Metasmart DryTM (isopropyl ester of the hydroxylated analogue of methionine adsorbed onto a silicon dioxide carrier; Adisseo). Starting from 45 days p.p., animals from both groups were submitted to an ovum pickup (OPU) session once a week for at least 8 weeks. Collected cumulus–oocyte complexes (COC) were subjected to a standard invitro production (IVP) protocol (Stinshoff et al. 2014 Reprod. Fertil. Dev. 26, 502–10) including IVM, IVF, and invitro culture (IVC). Cleavage and developmental rates up to the morula/blastocyst stage were recorded on Days 3, 7, and 8. In total, 1211 follicles have been aspirated from RPAA animals compared with 1413 from CON animals, from which 742 and 885 COC were collected, respectively. The calculated recovery rate based on the number of aspirated follicles and collected COC was similar for both groups (61.3±29.4% vs. 62.6±33.5%). Cleavage and developmental rates based on 240 (RPAA group) and 299 (CON group) COC also showed similar results [RPAA: 84.1±5.9% (202/240), 18.3±4.4% (44/240), 18.8±4.7% (45/240); CON: 81.9±8.6% (245/299), 15.4±8.9% (46/299), 16.7±8.4% (50/299)]. In conclusion, supplementation of RPAA (methionine and lysine) had no beneficial effect on the developmental competence of COC obtained from these animals compared with those collected from cows fed the diet without RPAA supplementation.
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Ritz, M., A. Gonzalez, A. S. Fries, T. Scheu, N. Blad-Stahl, F. Kotarski, G. Schuler, C. Koch, and C. Wrenzycki. "38 Developmental competence of bovine cumulus–oocyte complexes collected from cows fed rumen-protected methionine and lysine." Reproduction, Fertility and Development 33, no. 2 (2021): 126. http://dx.doi.org/10.1071/rdv33n2ab38.
Full textAbstract:
Supplementation of rumen-protected amino acids (RPAA) has proven to be an effective tool to supply limiting AA in dairy diets. Methionine and lysine are the two most limiting AA for lactating dairy cows. Recently, it has been shown that methionine supplementation seems to affect pre-implantation embryos collected from superovulated cows enhancing their developmental competence because there is strong evidence that endogenous lipid reserves serve as an energy substrate (Acosta et al. 2016 Theriogenology 85, 1669–1679). Moreover, higher concentrations of methionine were determined in the follicular fluid of the first dominant follicle postpartum in cows supplemented with rumen-protected methionine and rumen-protected choline from 21 days before calving to 30 days postpartum and it was assumed that higher methionine concentrations in the follicular fluid could affect oocyte quality (Acosta et al. 2017 Theriogenology 96, 1–9). There is no information available so far regarding the effect of a combined methionine and lysine supplementation (each rumen-protected) on oocyte quality. Therefore, the objective of this study was to evaluate the effect of a combined methionine and lysine supplementation during early to mid-lactation on the developmental competence of oocytes collected from lactating dairy cows (days 0 to 100 p.p.). Thirty pregnant multiparous German Holstein dairy cows were grouped 3 weeks before their expected calving date, receiving identical diets. After calving, they were randomly allocated to 2 groups fed a total mixed ration supplemented with (N=14 cows; RPAA) or without (N=16 cows; CON) LysiGEMTM (encapsulated lysine; Kemin Industries) and Metasmart DryTM (isopropyl ester of the hydroxylated analogue of methionine adsorbed onto a silicon dioxide carrier; Adisseo). Starting from 45 days p.p., animals from both groups were submitted to an ovum pickup (OPU) session once a week for at least 8 weeks. Collected cumulus–oocyte complexes (COC) were subjected to a standard invitro production (IVP) protocol (Stinshoff et al. 2014 Reprod. Fertil. Dev. 26, 502–10) including IVM, IVF, and invitro culture (IVC). Cleavage and developmental rates up to the morula/blastocyst stage were recorded on Days 3, 7, and 8. In total, 1211 follicles have been aspirated from RPAA animals compared with 1413 from CON animals, from which 742 and 885 COC were collected, respectively. The calculated recovery rate based on the number of aspirated follicles and collected COC was similar for both groups (61.3±29.4% vs. 62.6±33.5%). Cleavage and developmental rates based on 240 (RPAA group) and 299 (CON group) COC also showed similar results [RPAA: 84.1±5.9% (202/240), 18.3±4.4% (44/240), 18.8±4.7% (45/240); CON: 81.9±8.6% (245/299), 15.4±8.9% (46/299), 16.7±8.4% (50/299)]. In conclusion, supplementation of RPAA (methionine and lysine) had no beneficial effect on the developmental competence of COC obtained from these animals compared with those collected from cows fed the diet without RPAA supplementation.
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Oguntoye, M. A., O. M. O. Idowu, and A. O. Oso. "Performance and carcass characteristics of broiler chickens fed maize-cassava diets supplemented with methionine and inorganic sulphur." Nigerian Journal of Animal Production 44, no. 1 (December 24, 2020): 233–44. http://dx.doi.org/10.51791/njap.v44i1.789.
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The major challenges of cassava supplementation in poultry diets are high cyanide and low protein content. This study was conducted to evaluate the nutritional value of maize-cassava diets supplemented with methionine and inorganic sulphur for broiler chickens. Atotal of 270 one day-old broiler chicks were randomly assigned to nine treatment groups of 30 birds with 10 birds per replicate. Starter and finisher diets were formulated and fed for a period of 56 days. Dietary treatments were subjected to completely randomized design. The birds were fed diets supplemented with 0, 125, 250 mg/kg DL-methionine and 0, 125, 250 mg/kg inorganic sulphur from Cu4SO .There was a linear increase in final weight (FW) and total weight gain (TWG) with supplementation of 125mg/kg DL-methionine across dietary sulphur levels at the starter phase. Birds fed diets supplemented with 125 mg/kg DL-methionine and 250 mg/kg inorganic sulphur showed higher (p<0.05values of 463.23g, 424.66g and 2.66 for FW, TWG and feed conversion ratio respectively. The finisher phase followed a similar trend. Carcass yield showed significant (p<0.05) improvement in dress weight, cut part and organ weight in birds fed diets supplemented with 125 mg/kg DL-methionine and 250 mg/kg inorganic sulphur. The findings revealed that birds with diets supplemented with125 mg/kg DL-methionine and 250 mg/kg inorganic sulphur showed improved growth performance and carcass yield. In conclusion from this study supplementation of DL-methionine and inorganic sulphur in diets for broiler chickens resulted in improved growth performance and carcass yield.
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de Sousa, Fabiana Cristina Belchior, Ana Paula Del Vesco, Vittor Zancanela, Thais Pacheco Santana, Angélica de Souza Khatlab, Víctor Emanuel Meneses Feitosa, Claudson Oliveira Brito, Leandro Teixeira Barbosa, and Eliane Gasparino. "Effects of methionine as free amino acid and dipeptide on productive efficiency and meat quality of broilers under acute and chronic heat stress." Animal Production Science 62, no. 4 (December 21, 2021): 342–57. http://dx.doi.org/10.1071/an20592.
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Context Methionine in the form of free amino acid has been widely studied in broilers challenged by heat stress (HS). However, the effects of methionine dipeptide in broilers subjected to HS are not known. Aims To evaluate the effects of methionine as free amino acid and dipeptide on the performance, oxidative status, plasma parameters and meat quality of broilers subjected to acute and chronic HS. Methods Broilers were evaluated at the following three experimental periods: 24 h of evaluation (21–22 days of age); 10 days of evaluation (22–32 days of age); and 20 days of evaluation (22–42 days of age). Broilers were divided into two groups; one group was raised in thermal comfort, and the other group was raised in continuous HS of 30°C. In both groups, animals received a diet without methionine supplementation (MD), with supplementation of methionine as free amino acid (dl-M), and with supplementation of methionine as dipeptide (dl-MM). Key results HS reduced body weight gain after 10 and 20 days of evaluation. Broilers under HS condition fed the MD diet had the highest concentrations of thiobarbituric acid reactive substances (TBARS) at 22 days of age and carbonylated proteins (CP) at 32 days of age. At 42 days of age, broilers fed the MD diet had higher concentrations of TBARS and CP. At 32 days of age, broilers under HS had lower high-density lipoprotein and higher low-density lipoprotein concentrations. In breast meat, broilers in thermal comfort fed the dl-M or dl-MM diets had a lower cooking loss. Broilers in HS fed the dl-M diet had the lowest cooking loss. HS reduced the pH of the meat of legs. Conclusions The acute and chronic HS affect the broiler performance in different ways. Methionine supplementation contributes to reduce the effects of HS. There were no notable differences between the supplementation of dl-M or dl-MM. Implications Productivity and the quality of the chicken meat are the most important attributes of the production. The ambient temperature can influence these parameters. The methionine in its most varied forms, due to its direct and indirect antioxidant function, has been shown to be an effective source of protection for the animal in adverse conditions such as during HS.
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Velazquez, Milca Rosa, Fernanda Batistel, Juan Pinos Rodriguez, and Alejandro E. Relling. "PSIV-B-26 Late-Breaking: Polyunsaturated fatty acids and methionine supplementation during late gestation alters fetal liver development in sheep." Journal of Animal Science 97, Supplement_3 (December 2019): 321–22. http://dx.doi.org/10.1093/jas/skz258.646.
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Abstract Supplementation with polyunsaturated fatty acids or methionine have a fetal programming effect in mammals. However, the effect of both nutrients on fetal development and DNA methylation have not been explored. The objective of this study was to evaluate the effects of supplementing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), methionine, or both to ewes during late gestation on fetus development and liver global DNA methylation. Twenty ewes (n = 5) were supplemented from day 100 to 145 of gestation. The treatments were: 1) control diet (CONT) without EPA and DHA supplementation (PUFA) or methionine supplementation (MET); 2) control plus PUFA (1 % Ca salts of fatty acids intake, Strata G113®); 3) control plus MET (0.1 % rumen protected methionine intake, Smartamine®); and 4) control plus PUFA and MET (PUFA+MET). On day 145, ewes were euthanized and fetuses were removed. Ewe, fetus, placenta, and fetal liver were weighed. Plasma samples were collected from ewes and fetuses to measure concentration of glucose and NEFA. Liver global DNA methylation was analyzed. Data was analyzed as a complete randomized experiment with a 2x2 factorial arrangement of treatments. Ewe supplementation did not affect (P > 0.1) fetus and placental weight. There was an interaction of treatment effect (P = 0.02) on fetal liver weight and ewe BW; CONT and PUFA+MET were grater that PUFA and MET. There were no differences (P > 0.10) on ewe and fetus NEFA as well as fetus glucose, but MET increased (P = 0.03) ewe plasma glucose concentration. Both treatments affected liver global DNA methylation of fetus (main effects P < 0.01; interaction P = 0.32); all the treatments increased global DNA methylation compared, with CONT, and PUFA+MET has greater than PUFA and MET. In conclusion PUFA and methionine supplementation in late gestation modified fetus liver development and DNA methylation.
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Reda, Fayiz M., Ayman A. Swelum, Elsayed O. S. Hussein, Shaaban S. Elnesr, Ahmad R. Alhimaidi, and Mahmoud Alagawany. "Effects of Varying Dietary DL-Methionine Levels on Productive and Reproductive Performance, Egg Quality, and Blood Biochemical Parameters of Quail Breeders." Animals 10, no. 10 (October 9, 2020): 1839. http://dx.doi.org/10.3390/ani10101839.
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The present study was carried out to study the effects of varying dietary DL-methionine (0, 0.5, 1.5, 2.5, and 3.5 g/kg) levels on the productive and reproductive performance, egg quality and blood biochemical parameters of quail breeders. In total, 150 mature Japanese quails at eight weeks of age were randomly allotted to five groups of 30 for each group. Each group included five replicates, each of six quails (four females and two males). The results showed that egg number, egg weight and egg mass were higher (p < 0.05) with the addition of all DL-methionine levels than that of the control group. Quails from the control group had a lower feed intake (p < 0.001) and a worse feed conversion ratio (FCR) than those from the DL-methionine-treated groups. Supplementation of DL-methionine up to 2.5 g/kg in quail diets increased fertility and hatchability percentages. Birds fed DL-methionine at 1.5 g/kg had the best egg production indices, better FCR and the highest values of fertility and hatchability. Egg weight, yolk %, Haugh unit, egg shape index and unit surface shell weight (USSW) were increased and eggshell % was decreased in quail supplemented with DL-methionine levels compared with the control quail (p < 0.05). Dietary DL-methionine levels did not affect (p > 0.05) the hemoglobin (Hb), red blood cells (RBCs), white blood cells (WBCs) and packed cell volume (PCV) of quails. DL-methionine levels (0.5 and 2.5 g/kg) augmented lymphocytes and basophile (p < 0.05). Low DL-methionine levels (0.5 or 1.5 g/kg) improved liver enzymes and kidney functions. Dietary DL-methionine levels (except 3.5 g/kg) declined serum lactate dehydrogenase (LDH) and decreased lipid profile parameters (except high-density lipoprotein—HDL). Supplementation of DL-methionine at 0.5 and 1.5 g/kg increased immunoglobulin (IgG, IgM and IgA) and superoxide dismutase (SOD), total antioxidant capacity (TAC), catalase (CAT) and reduced glutathione (GSH) (p < 0.001) compared with the control. In conclusion, dietary supplementation of DL-methionine (1.5 g/kg) can enhance the reproductive performance and egg quality of quail breeders. DL-methionine use at levels of 0.5 or 1.5 g/kg improved the liver and kidney functions, lipid profile, immunity and antioxidant parameters of Japanese quail.
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Vieira, Danilo Vargas Gonçalves, Thiago De Sousa Melo, José Humberto Vilar da Silva, Fernando Guilherme Perazzo Costa, Danilo Teixeira Cavalcante, Matheus Ramalho de Lima, Talita Pinheiro Bonaparte, José Geraldo de Vargas Júnior, Marilú Santos Sousa, and Ana Carolina Müller Conti. "Order of amino acid inclusion in the diet of DeKalb White laying hens." Semina: Ciências Agrárias 37, no. 3 (June 22, 2016): 1539. http://dx.doi.org/10.5433/1679-0359.2016v37n3p1539.
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Three hundred and twenty-four DeKalb White laying hens aged 42 weeks were distributed in a completely randomised design with nine treatments and six replicates of six birds in each treatment. The experiment lasted 112 days. Diets were: T1 = 16.02% crude protein - CP [Met + Lys + Thr + Trp + Val]; T2 = 14.02% CP [Met + Lys + Thr + Trp + Ile + Val]; T3 = 14.02% CP [no amino acid supplementation]; T4 = 14.02% CP [Met + Lys + Thr + Trp]; T5 = 14.02% CP [Met + Lys + Thr]; T6 = 14.02% CP [Met]; T7 = 14.02% CP [Lys]; T8 = 14.02% CP [Thr]; T9 = 14.02% CP [Trp]. Regarding the quality of the eggs, the percentage of yolk and albumen, shell thickness and Haugh unit were not affected by the different diets. The percentage of shell, specific gravity and albumen height showed significant differences. We found that supplementation of only one amino acid in the diet (T7, T8 or T9), with the exception of methionine (T6), worsened performance relative to the control. Supplementation of three amino acids (methionine, lysine and threonine; T5) or four amino acids (methionine, lysine, threonine and tryptophan; T4) worsened egg production and conversion per mass and per dozen eggs; however, feed intake and egg weight and mass were similar to the control treatment. When all amino acids (methionine, lysine, threonine, tryptophan, isoleucine and valine; T2) were supplemented performance was similar to the control treatment in all variables. Supplementation of methionine, lysine and threonine is essential for birds in the laying phase; however the addition of six essential amino acids (lysine, methionine, threonine, tryptophan, valine and isoleucine) to the diet of laying hens is important for a good productive performance comparable with the control treatment T1. However, the inclusion of the latter two (isoleucine and valine) is justified only if the production cost is lower.
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Lisnahan, Charles V., Wihandoyo Wihandoyo, Zuprizal Zuprizal, and Sri Harimurti. "PENGARUH SUPLEMENTASI DL-METIONIN DAN L-LISIN HCL PADA PAKAN STANDAR KAFETARIA TERHADAP BERAT BADAN, ORGAN DALAM DAN ORGAN REPRODUKSI AYAM KAMPUNG FASE PULLET." JURNAL ILMIAH PETERNAKAN TERPADU 6, no. 2 (July 30, 2018): 128. http://dx.doi.org/10.23960/jipt.v6i2.p128-133.
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The aim of this study was to determine the effect of the supplementation of dl-methionine and l-lysine HCl to diet based on cafeteria standards of native chickens on body weight, internal organs and reproductive organs of native chicken at pullet phase. A total of 128 fourteen weeks-old native chickens were used in this study. The native chickens were divided into 4 treatments with 4 replications. The dietary treatments were: T0 (ration based on cafeteria standard), T1 (ration based on protein standard of NRC), T2 (cafeteria ration+0.09% DL-Methionine+0.22% L-Lysine HCl), T3 (cafeteria ration+0.19% dl-methionine+0.42% l-lysine HCl). The data collected were body weight, liver and bile, pancreas, gizzard, testicular and ovary weight of native chickens aged 20 weeks. The result of this study for T0, T1, T2 and T3 showed that body weight of chickens were 1418.60; 1431.59; 1503.88 and 1556.41 g/bird, liver and bile weights were 22.44; 21.79; 24.49 dan 25.93 g/bird, pancreas weights were 2.33; 2.28; 2.50 and 2.70 g/bird, gizzard weight were 25.31; 23.91; 25.38 and 26.86 g/bird, testicular weight were 3.66; 3.86; 9.55 and 9.46 g/bird, testicular volume were 3.81; 3.38; 9.13 and 9.16 and ovary weight were 1.12; 1.32; 3.14 and 4.61 g/bird, respectively. The results showed that supplementation of DL-Methionine and L-Lysine HCl gave significantly effect (P<0.05) upon body weight, liver and bile weight, pancreas weight, testicular weight and volume and ovary weight, but it did not effected to gizzard weight. It can be concluded that supplementation of 0.27% dl-methionine and 0.79% l-lysine HCl increase body weight, internal organs weight and reproductive organs weight of native chickens. Keywords: Cafeteria, DL-Methionine, L-Lysine HCl, Native Chicken, Supplementation.
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Banton, Sydney, Julia G. Pezzali, Renan Antunes Donadelli, Marica Bakovic, Katharine M. Wood, Adronie Verbrugghe, and Anna-Kate Shoveller. "83 The acute effects of feeding a grain-free diet with or without methionine, taurine or methyl donor/acceptor supplementation on plasma and whole blood amino acid concentrations in dogs." Journal of Animal Science 98, Supplement_4 (November 3, 2020): 62. http://dx.doi.org/10.1093/jas/skaa278.112.
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Abstract Grain-free pet foods have been sold for over a decade and comprise more than 40% of dog and cat diets sold today. Grain-free diets replace grain ingredients with pulse ingredients, which are high in lysine but low in methionine and cysteine, the precursor amino acids to taurine synthesis in the dog. The objective of this study was to evaluate the postprandial response of plasma methionine and taurine and whole blood taurine concentrations of dogs fed a grain-free diet without supplementation (CON) or with methionine (MET), taurine (TAU) or creatine, carnitine and choline (CCC) supplementation. Eight Beagles were pair housed and fed one of the four experimental diets for seven days in a 4x4 Latin Square Design. On the morning of d 7, cephalic catheters were placed and one fasted sample (0 min) and nine post-meal blood samples (15, 30, 60, 90, 120, 180, 240, 300 and 360 min) were collected. Data were analyzed as repeated measures using the PROC GLIMMIX function in SAS (Version 9.4). Dogs supplemented with MET had significantly higher plasma methionine concentrations from 30 to 360 minutes post-meal compared to dogs on CON, TAU and CCC treatments (P < 0.05). However, no differences were observed in plasma methionine concentrations between CON, TAU and CCC treatments at any time point (P > 0.05). Plasma taurine concentrations were significantly higher across time points in all treatment groups compared to CON (P < 0.05). Whole blood taurine concentrations tended to be higher across time points in MET and TAU treatment groups compared to CCC (P = 0.0513). Overall, MET, TAU and CCC supplementation increased plasma taurine concentrations compared to CON, but only MET supplementation increased plasma methionine concentrations from 30 to 360 minutes post-meal.
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Griep Júnior, Dirceu Neutzling, Adriana Aparecida Pereira, Gregório Murilo Oliveira Oliveira Júnior, Vitor Visintin Silva de Almeida, Edlaine Ferreira da Silva, Wilson Araújo da Silva, Rosineide Silva de Alcântara, Wanderson Alves da Silva, Gilmar Silva Cardoso Júnior, and Clístenes Gomes de Oliveira. "Choline and digestible methionine + cystine supplementation for quail in the laying phase." Semina: Ciências Agrárias 41, no. 1 (January 10, 2020): 305. http://dx.doi.org/10.5433/1679-0359.2020v41n1p307.
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The aim of this study was to evaluate the effect of levels of choline and digestible methionine + cystine in diets for quail in the laying phase. A total of 280 Japanese laying quail were assigned to one of eight treatments in a completely randomized design in a 4 × 2 factorial arrangement consisting of dietary supplementation with four levels of choline (0.0, 0.042, 0.084 and 0.126%) and two levels of digestible methionine + cystine (0.857 and 0.685%), with five replicates and seven birds per plot. The experimental period was 84 days, represented by four 21-day evaluation cycles in which quail performance and egg quality were evaluated. There was no interaction effect between the factors for any of the studied variables, except quail weight. The highest tested levels of methionine + cystine and choline supplementation improved egg weight, feed conversion egg mass?1 and yolk percentage. By contrast, albumen percentage decreased linearly as choline inclusion in the diet was elevated. The choline supplementation levels induced a quadratic response from eggshell percentage, whose optimum result was estimated at the choline level of 0.05% in the diet. Egg specific gravity decreased linearly as choline supplementation was increased. The indicated level of digestible methionine + cystine in the diet of Japanese quail in the laying phase is 0.857%, while choline should be supplemented at 0.126%.
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Griep Júnior, Dirceu Neutzling, Adriana Aparecida Pereira, Gregório Murilo Oliveira Oliveira Júnior, Vitor Visintin Silva de Almeida, Edlaine Ferreira da Silva, Wilson Araújo da Silva, Rosineide Silva de Alcântara, Wanderson Alves da Silva, Gilmar Silva Cardoso Júnior, and Clístenes Gomes de Oliveira. "Choline and digestible methionine + cystine supplementation for quail in the laying phase." Semina: Ciências Agrárias 41, no. 1 (January 10, 2020): 305–12. http://dx.doi.org/10.5433/1679-0359.2020v41n1p305.
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The aim of this study was to evaluate the effect of levels of choline and digestible methionine + cystine in diets for quail in the laying phase. A total of 280 Japanese laying quail were assigned to one of eight treatments in a completely randomized design in a 4 × 2 factorial arrangement consisting of dietary supplementation with four levels of choline (0.0, 0.042, 0.084 and 0.126%) and two levels of digestible methionine + cystine (0.857 and 0.685%), with five replicates and seven birds per plot. The experimental period was 84 days, represented by four 21-day evaluation cycles in which quail performance and egg quality were evaluated. There was no interaction effect between the factors for any of the studied variables, except quail weight. The highest tested levels of methionine + cystine and choline supplementation improved egg weight, feed conversion egg mass‒1 and yolk percentage. By contrast, albumen percentage decreased linearly as choline inclusion in the diet was elevated. The choline supplementation levels induced a quadratic response from eggshell percentage, whose optimum result was estimated at the choline level of 0.05% in the diet. Egg specific gravity decreased linearly as choline supplementation was increased. The indicated level of digestible methionine + cystine in the diet of Japanese quail in the laying phase is 0.857%, while choline should be supplemented at 0.126%.
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Ratriyanto, Adi, and Susan Dikta Mentari. "Pertumbuhan dan efisiensi pakan ayam broiler betina yang diberi pakan mengandung metionin cukup dan disuplementasi betain." Jurnal Ilmu-Ilmu Peternakan 28, no. 3 (December 10, 2018): 233. http://dx.doi.org/10.21776/ub.jiip.2018.028.03.06.
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Betaine is a methyl group donor that can increase protein synthesis since it has a similar role as methionine. The study was performed to determine the supplementation effect of betaine in the diet containing adequate methionine on growth performance and feed efficiency of female broilers. In total, 200 of day-old chicks of female broiler chickens were allotted to 4 treatments with 5 replicationsof 10 birds. This experiment was designed as completely randomized design. Control diet was formulated to contain adequate methionine (0.50% for starter and 0.38% for finisher period). The other dietary treatments were obtained by supplementing 0.1%, 0.2% and 0.3% betaine. The variables observed consisted of feed, protein and energy consumption, average daily weight gain, feed efficiency, protein and energy efficiency ratio. Supplementation of 0.1% betaine generated a higheraverage daily weight gain (P<0.05), but supplementation at higher levels (0.2 and 0.3%) had no effect on weight gain. However, betaine did not affect feed, energy and protein consumption, as well as feed efficiency and energy and protein efficiency ratio. Thus, betaine can be applied in the diet containing adequate methionine at the level of 0.1% to produce higherdaily weight gain without affecting feed efficiency. Supplementation of betaine at higher levels than 0.1% did not show positive effect on weight gain and feed efficiency in female broiler chickens.
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Storch, K. J., D. A. Wagner, J. F. Burke, and V. R. Young. "[1-13C; methyl-2H3]methionine kinetics in humans: methionine conservation and cystine sparing." American Journal of Physiology-Endocrinology and Metabolism 258, no. 5 (May 1, 1990): E790—E798. http://dx.doi.org/10.1152/ajpendo.1990.258.5.e790.
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Methionine (Met) conservation in healthy young adult men (4/diet group) was explored by supplying one of the following three L-amino acid based diets: 1) adequate Met but no cystine; 2) neither Met nor cystine; or 3) no Met but cystine supplementation. After 5 days, subjects received a continuous intravenous infusion of L-[1-13C; methyl-2H3]Met for 5 h while the diet was given as small isocaloric isonitrogenous meals. Estimates were made of rates of Met incorporation into protein synthesis (S) and release from body proteins (B), transmethylation (TM), remethylation of homocysteine (RM), and transsulfuration (TS). For the adequate Met diet, the rates were S = 24 +/- 2, B = 18 +/- 1, TM = 12.4 +/- 1.7, RM = 4.7 +/- 1.1, and TS = 7.6 +/- 0.6 (SE) mumol.kg-1.h-1. The sulfur amino acid-devoid diet significantly (P less than 0.05) reduced S, TM, RM, and TS. Supplementation of this diet with cystine reduced Met oxidation (P = 0.05). Therefore, two loci are quantitatively important regulatory points in Met conservation in vivo: 1) the distribution of Met between the pathways of protein anabolism and TM (Met locus) and 2) the distribution of homocysteine between RM and TS (homocysteine locus).
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Kurz, Katharina, Barbara Frick, Christina Fürhapter, Günter Weiss, Barbara Wirleitner, Norbert Sepp, and Dietmar Fuchs. "Homocysteine metabolism in different human cells." Pteridines 24, no. 3 (December 1, 2013): 183–89. http://dx.doi.org/10.1515/pterid-2013-0039.
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AbstractThe effects of cytokine and mitogen stimulation on homocysteine (HCY) metabolism in different cells were investigated: in human dermal microvascular endothelial cells (HDMEC), T lymphocytes, mature and immature dendritic cells, and myelomonocytic (THP-1) and monocytic cell lines (U-937). Furthermore, the influence of supplementation of cells with folate acid, methionine and the combination of both on HCY metabolism was investigated. Unstimulated HDMEC and dendritic cells only produced very little amounts of HCY, and stimulation did not change HCY formation significantly either. However, higher HCY concentrations were detected in HDMEC and dendritic cells under supplementation with methionine and slightly less under supplementation with folate. Proliferating T lymphocytes showed an increase in HCY production on stimulation with increasing doses of mitogens; proliferative activity was associated with HCY formation. THP-1 and U-937 cells produced significantly more HCY than endothelial cells; U-937 cells produced most HCY, which was mainly due to their high proliferation rate. Stimulation of both cell lines with lipopolysaccharide and interferon-γ, respectively, showed a significant effect on HCY production of cells; in THP-1 cells, stimulation with IFN-γ and lipopolysaccharide induced neopterin formation. Methionine supplementation strongly increased and folate supplementation slightly decreased HCY formation in both cell lines. Thus, inflammation may play a role in moderate hyperhomocysteinemia.
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Ogunwole, Olugbenga Adeniran, B. C. Majekodunmi, R. A. Faboyede, and D. Ogunsiji. "Meat and Bone Characteristics of Broiler Chickens Fed Groundnut Cake-Based Diets as Affected by Graded Dietary Supplements of Crystalline L- Lysine and DL- Methionine." JOURNAL OF ADVANCES IN AGRICULTURE 6, no. 1 (April 30, 2016): 846–52. http://dx.doi.org/10.24297/jaa.v6i1.5392.
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Effects of supplemental dietary lysine and methionine in a Groundnut Cake (GNC) based diets on meat and bone characteristics of broiler chickens were investigated. In a completely randomized design, a total of 168 one - day – old Arbor acre broiler chicks were randomly allocated to seven dietary treatments each in triplicate of eight birds per replicate. The Seven starter and finishers’ diets were: GNC based diets without any amino acid (lysine or methionine) supplementation (T1); GNC diet + 0.2% lysine (T2); GNC diet + 0.4% lysine (T3); GNC diet + 0.2% methionine (T4); GNC diet + 0.4% methionine (T5); GNC diet + 0.2 lysine and 0.2% methionine (T6) and GNC diet + 0.4% lysine and 0.4% methionine (T7). Experimental diets and water were offered to birds ad libitum in an experiment lasting six-week. At day 42, two birds per replicate were slaughtered, meat and bone characteristics determined. There were significant variations (P<0.05) in the crude protein (%) and ether extract (%), pH1 and pH2 of meat. Thiobarbituric acid reactive substances composition of meat at days 0, 5, and 10 were similar (P<0.05) and were not affected by dietary amino acid supplementation. Tibiotarsal index (mg/mm) of bone (22.10, 27.25, 33.35, 31.40, 28.70, 31.45 and 29.75 for broilers on T1, T2, T3, T4, T5, T6 and T7, respectively) were increased significantly (P<0.05) by amino acid supplementation. Significantly differences (P<0.05) were observed in the calcium, phosphorus and potassium (%) contents of broilers’ bone across treatments. Supplemental lysine and both lysine and methionine improved meat quality and bone development of broiler chickens in this study.
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Ratriyanto, Adi, Rysca Indreswari, and Sudiyono Sudiyono. "EFEKTIVITAS BETAIN PADA PAKAN AYAM BROILER RENDAH METIONIN BERDASARKAN PARAMETER BERAT BADAN DAN KARKAS." Caraka Tani: Journal of Sustainable Agriculture 27, no. 1 (September 11, 2017): 1. http://dx.doi.org/10.20961/carakatani.v27i1.14342.
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<p>This experiment was conducted to investigate the efficacy of methyl groups donor of betaine to spare methionine based on performance and carcass in broiler chickens. In total, 100 day old broiler chickenss were used in this experiment. They were allocated to 4 dieaty treatmants in 5 replicates with 5 broilers each. The basal diet was deficient in methionine. The control diet was obtained from basal diet supplemented with methionine to meet the requirements 5 g/kg diet for starter period and 3.8 g/kg diet for finisher period. The rest 3 treatments were basal diet deficient in methionine supplemented with 1, 2 and 3 g betaine per kg diet. The variables that were observed included average daily gain and carcass characteristics. The data were than analyzed by analysis of variance of Completely Randomized Design and continued with Dunnet test. The result indicated that supplementation of 1 and 2 g betaine per kg diet deficient in methionine resulted the broiler performance which was not different with supplementation of methionine. Accordingly, carcass characteristics for broilers fed diet supplemented with methionine were not different with that supplemented with betaine, which indicated that betaine may reduce the methionine level in the broiler diet. It can be concluded that betaine spares metionine in broiler diets.</p>
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Ohh, Sang Jip, and Joon Yeop Lee. "Dietary Chromium-methionine Chelate Supplementation and Animal Performance." Asian-Australasian Journal of Animal Sciences 18, no. 6 (November 26, 2005): 898–907. http://dx.doi.org/10.5713/ajas.2005.898.
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Saki, A. A., H. A. Mohammad P, A. Ahmdi, M. T. Akhzar, and M. M. Tabatabie. "Decreasing Broiler Crude Protein Requirement by Methionine Supplementation." Pakistan Journal of Biological Sciences 10, no. 5 (February 15, 2007): 757–62. http://dx.doi.org/10.3923/pjbs.2007.757.762.
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Tripp, M. W., T. A. Hoagland, G. E. Dahl, A. S. Kimrey, and S. A. Zinn. "Methionine and somatotropin supplementation in growing beef cattle." Journal of Animal Science 76, no. 4 (1998): 1197. http://dx.doi.org/10.2527/1998.7641197x.
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Ohh, Sang-Jip, Chang-Hyeuk Kim, Jong-Seo Shin, Kyung-Il Sung, and Hyun-Sook Kim. "Chromium Methionine Supplementation Decreases Obesity Indices in Rats." Preventive Nutrition and Food Science 8, no. 3 (September 1, 2003): 249–52. http://dx.doi.org/10.3746/jfn.2003.8.3.249.
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