Journal articles: 'Methanol'

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Tselishchev, Oleksii, Ayodeji Ijagbuji, Maryna Loriia, and Vanadii Nosach. "Synthesis of Methanol from Methane in Cavitation Field." Chemistry & Chemical Technology 12, no. 1 (March 21, 2018): 69–73. http://dx.doi.org/10.23939/chcht12.01.069.

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Rajendrasozhan, Saravanan, Hani El Moll, Mejdi Snoussi, Ramy M. Romeilah, Emad A. Shalaby, Kareem M. Younes, and Hossam S. El-Beltagi. "Phytochemical Screening and Antimicrobial Activity of Various Extracts of Aerial Parts of Rhanterium epapposum." Processes 9, no. 8 (July 31, 2021): 1351. http://dx.doi.org/10.3390/pr9081351.

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Rhanterium epapposum, native to the Arabian Peninsula, is traditionally used to cure skin infections. The objective is to screen the phytochemical content and antimicrobial activity of aqueous, methanol and 80% methanol extracts of aerial parts of R. epapposum. The phytochemical screening of aqueous, methanolic, and 80% methanol extracts of R. epapposum was conducted using gas chromatographymass spectrometry. The antimicrobial activities of the extracts were assessed by well diffusion and microdilution methods. Qualitative phytochemical analysis revealed the presence of 2-methoxy-4-vinylphenol in all three extracts, whereas ethanol, 2-methoxy-, acetate; n-hexadecanoic acid; and 2,3-butanediol are present in higher amount exclusively in the methanol, 80% methanol and aqueous extracts of the aerial parts of R. epapposum, respectively. The highest antibacterial activity was shown by the aqueous extract S. aureus, P. aeruginosa, E. cloacae, and K. pneumoniae, methanolic extract against S. aureus, methicillin-resistant S. aureus, and E. coli, and 80% methanol extract against S. epidermidis, and S. paucimobilis. Interestingly, 80% methanol extracts showed the highest antifungal activity against C. albicans, C. guillermondii, C. vaginalis, C. utilis, and C. tropicalis. The aerial parts of R. epapposum showed broad-spread antimicrobial activity against bacteria and fungi. Especially, the 80% methanol extract showed potent antifungal activity against all the tested fungal strains.

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Karouche, S., S. Karouche, A. Benbott, A. Benbott, S. Henouda, S. Malki, and I. Boudchicha. "Evaluation of phenolic content and biological activities of Bunium mauritanicum tuberss." Journal of Fundamental and Applied Sciences 12, no. 2 (May 24, 2023): 916–30. http://dx.doi.org/10.4314/jfas.v12i2.27.

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The present work is carried out to evaluate biological activities of tubers extracts of Bunium mauritanicum. The methanol extract yield had higher (7.81%) tha n that of the aqueous extract (6.79%). The quantitative analysis of total phenols and flavonoid revealed that the highest concentration was recorded for the methanoic fraction with 89,442 ± 5,951μg EAG mg and 4.031 ± 0.141 μg EQ mg of extract respectively. In addition, the aqueous extract of tubers represents the most important antioxidant activity with an IC 50 of 0.14 mg ml against 2,2 diphenyl 1 picryl hydrazyl (DPPH Wherea s, the two extracts of the tubers have low reducing capacities compared to standard with EC 50 equal to 0.048 mg/ ml for the methanolic extract, 0.018 mg ml for t he aqueous extract and 0.009 mg/ ml for ascorbic acid. The methano l ic and aqueous extracts of Bunium mauritanicum reacted positively at least on one of the bacterial strains studied.

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Weijma, J., and A. J. M. Stams. "Methanol conversion in high-rate anaerobic reactors." Water Science and Technology 44, no. 8 (October 1, 2001): 7–14. http://dx.doi.org/10.2166/wst.2001.0452.

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An overview on methanol conversion in high-rate anaerobic reactors is presented, with the focus on technological as well as microbiological aspects. The simple C1-compound methanol can be degraded anaerobically in a complex way, in which methanogens, sulfate reducing bacteria and homoacetogens interact cooperatively or competitively at substrate level. This interaction has large technological implications as it determines the final product of methanol mineralization, methane or carbon dioxide. The degradation route of methanol may be entirely different when environmental conditions change. Direct methanogenesis from methanol seems the predominant mineralization route under mesophilic conditions both in the absence and the presence of sulfate. Under thermophilic conditions methanol oxidation to carbon dioxide and hydrogen appears to play an important role. The UASB technology for mesophilic digestion of methanolic waste has presently reached full-scale maturity. The potential of methanol as feedstock for anaerobic processes is discussed.

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Hashim, N. A., H. Ya'akob, M. N. M. Rosdi, N. A. Zainol, F. Husin, H. H. Soib, and N. F. Norhisham. "Antioxidant properties of extracts and SPE fractions from Annona muricata leaves." Food Research 4, S2 (April 16, 2020): 71–75. http://dx.doi.org/10.26656/fr.2017.4(s2).s02.

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Annona muricata L. (Magnoliales: Annonaceae) is a tropical plant species known for its edible fruit and well known for the medicinal value. In this study, the antioxidant properties of A. muricata leaves is evaluated for different extracts and fractions. The airdried leaves of A. muricata leaves were successively extracted with n-hexane and methanol. The methanolic crude extract was further semi-purified by utilizing solid-phase extraction (SPE) in a mixture of mobile phase aqueous: methanol to methanol and resulted in four semi-purified fractions (ALM 1 – ALM 4). Antioxidant properties of extracts and fractions were evaluated by free radical scavenging activity against 1,1-Diphenyl-2- picrylhydrazyl (DPPH) radicals comparing with standard ascorbic acid, quercetin, and catechin. Results indicated that methanolic extracts showed exceptionally higher radical scavenging activity compared with n-hexane crude extract with IC50 44.21 µg/mL and 302.41 µg/mL, respectively. Meanwhile, fractions ALM 2 (aqueous: methanol; 50: 50) exhibited the highest scavenging activity with the IC50 of 12.46 µg/mL followed by ALM 1 (aqueous: methanol; 80: 20) with 51.44 µg/mL, ALM 3 (aqueous: methanol; 20: 80) with 54.39 µg/mL and ALM 4 (100% methanol) with 89.02 µg/mL, respectively. In conclusion, ALM 2 could be potentially applied as a natural antioxidant agent in both food, nutraceuticals or pharmaceuticals fields for health promotion. However, ALM 2 has to be further purified to study the constituents which possibly condensed mainly with phenolic and flavonoids that known to possess the highest antioxidant effects.

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Sharma, Leihaothabam Dinendra, Md Riyajuddin Khan, and Angom Kikim Devi. "Methanolic and ethanolic phytochemical screening of Sweet flag (Acorus calamus L.) rhizome." Journal of Drug Delivery and Therapeutics 13, no. 9 (September 15, 2023): 114–18. http://dx.doi.org/10.22270/jddt.v13i9.6223.

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Acorus calamus is a medicinal plant with several ethno-medicinal properties. The present study was carried to screen the presence of major phytochemical groups. Phytochemical screening of methanolic and ethanolic rhizome extract showed the presence of carbohydrates, alkaloids, phenolic compound, protein, amino acid flavanoids and tannins. The total phenolic content in methanolic and ethanolic rhizome extracts in terms of gallic acid equivalent was 4.77.50 and 7.5 mg/g of extract powder respectively whereas the total flavonoid content of methanolic and ethanolic extract was 12.02 and 12.89 mg QE/g of extract respectively. Total antioxidant activity was found highest in ethanol extract (75.6 µg AAE/mg of extract) than methanol extract (37.2 µg AAE/mg of extract). Reducing power is also found highest in ethanol extract than methanol extract. Keywords: Acorus calamus, phytochemical screening, methanol, ethanol.

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PERBONI, LAÍS TESSARI, DIRCEU AGOSTINETTO, CANDIDA RENATA JACOBSEN DE FARIAS, FÁBIO CLASEN CHAVES, and JÉSSICA RODRIGUES GARCIA. "ANTIFUNGAL ACTIVITY OF Heteranthera reniformis EXTRACTS AGAINST Bipolaris oryzae1." Revista Caatinga 34, no. 2 (June 2021): 339–49. http://dx.doi.org/10.1590/1983-21252021v34n210rc.

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ABSTRACT Plants synthesize specialized metabolites to defend themselves against pathogens. These active compounds, when isolated and identified, can be used as template for fungicide development. Based on field observations, Heteranthera reniformis (kidney leaf mudplantain) could potentially synthesize compounds with antifungal activity. The goal of this study was to evaluate the fungicidal activity of H. reniformis leaf extracts on Bipolaris oryzae development. The activities of hexane, ethyl acetate, and methanol extracts of H. reniformis leaves were tested on mycelial growth, sporulation, and colony morphology. Due to the highest inhibition of B. oryzae sporulation, methanol extract was chosen for concentration tests. The effect of methanol extract on B. oryzae spore length and germination was also evaluated. Methanolic extract was the most active in inhibiting B. oryzae sporulation. The morphology of the colonies is altered when the fungus grows in medium containing H. reniformis leaf extracts. Higher concentration of methanol extract stimulates mycelial growth and suppresses B. oryzae sporulation. There are reductions in length and number of germinated B. oryzae spores caused by methanol extract of H. reniformis leaves. Methanolic extract has compounds with antifungal activity and should be subjected to bioassay-guided isolation for purification and identification of these active compounds.

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Binuwangi, Amugoda Kankanamge Dona Malsha, Madinage Pawani Jayathri Perera, Asurumunige Anushka Gayathrie Silva, Renuka Nilmini Attanayake, and Chandima Shashikala Kumari Rajapakse. "Antioxidant, a-Amylase Inhibitory Activities and Photoprotective Properties of Peels of Nephelium Lappaceum Linn. (Malwana Special)." Oriental Journal Of Chemistry 37, no. 2 (April 30, 2021): 499–507. http://dx.doi.org/10.13005/ojc/370233.

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This study focused on evaluation of antioxidant, α-amylase inhibitory activities and photo protective properties of peels of Nephelium lappaceum Linn. (rambutan); Malwana special. Methanolic extract of peels was sequentially partitioned in hexane, dichloromethane (DCM) and aqueous methanol. The methanol extract showed a significantly (p < 0.05) higher DPPH radical scavenging activity than that of butylated hydroxytoluene. Among the fractions, the highest total phenolic content (TPC) was found in the aqueous methanol fraction. DCM and aqueous methanol fractions were rich in flavonoids. In vitro α-amylase inhibitory activity of the aqueous methanol fraction was also significantly higher than the standard drug, acarbose. Partially purified aqueous methanol fraction of rambutan peels exhibited UV-B absorption with a moderate solar protection factor. The results revealed that the peels of Nephelium lappaceum Linn., Malwana specialcan be considered as a promising source for the development of natural antioxidant, cosmeceutical sunscreen and antidiabetic agents.

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Lawrence, Reena, Kapil Lawence, Manisha Prasad, and Ritika Singh. "ANTIOXIDANT ACTIVITY OF METHANOL EXTRACT OF ZINGIBER OFFICINALE GROWN IN NORT INDIAN PLAINS." Scientific Temper 1, no. 01 (February 4, 2010): 117–20. http://dx.doi.org/10.58414/scientifictemper.2010.01.1.19.

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The present investigation evaluates the antioxidant activity of methanolof extract of Zingiber officinale (family-Zingiberaceae).The hydrogen donating abilityof methanol extract of ginger was measured by reduction of DPPH and potassiumferricyanide. It was observed that the antioxidant activity of methanol extractincreases proportionately with concentration.

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Tönges, Yannic, Vincent Dieterich, Sebastian Fendt, Hartmut Spliethoff, and Jakob Burger. "Techno-Economic Analysis of Large Scale Production of Poly(oxymethylene) Dimethyl Ether Fuels from Methanol in Water-Tolerant Processes." Fuels 4, no. 1 (January 6, 2023): 1–18. http://dx.doi.org/10.3390/fuels4010001.

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Poly(oxymethylene) dimethyl ether (OME) are a much-discussed and promising synthetic and renewable fuel for reducing soot and, if produced as e-fuel, CO2 emissions. OME production is generally based on the platform chemical methanol as an intermediate. Thus, the OME production cost is strongly dependent on the methanol cost. This work investigates OME production from methanol. Seven routes for providing methanolic formaldehyde solutions are conceptually designed for the first time and simulated in a process simulator. They are coupled with a state-of-the-art OME synthesis to evaluate the economics of the overall production chain from methanol to OME. For a plant size of 100 kt/a, the average levelized product cost of OME is 79.08 EUR/t plus 1.31 times the cost of methanol in EUR/t.

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Vikhe, Sunayana, Pravin Aladi, and Rahul Laxman Vikhe. "Antidiabetic and antihyperlipidemic effects of crude fractions from Chlorophytum borivilianum root methanolic extract on streptozotocin induced diabetic rats and phytochemical investigation by LCMS analysis." International Journal of Experimental Research and Review 38 (April 30, 2024): 26–36. http://dx.doi.org/10.52756/ijerr.2024.v38.003.

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To evaluate the in vitro and in vivo pharmacological efficacy of the plant Chlorophytum borivilianum in diabetes and hyperlipidemia and to confine and describe the synthetic constituents from the roots that are in charge of the action. The present study was carried out to investigate the ethno-medical use of Chlorophytum borivilianum root methanolic extract as a potential anti-diabetic and antihyperlipidemic agent in STZ-induced diabetic rats. Extract was tested for in vitro and in vivo biological activities. Soxhlet extraction was carried out using methanol as a solvent, and TLC and column chromatography were used for fractionation. Liquid Chromatography and Mass Spectroscopic study confirmed the structures of isolated compounds. Chlorophytum borivilianum root methanolic extract showed the presence of phytoconstituents as Dihydrocapsaicin, Reserpine, Deserpidine, Biliverdin-IX-α, and Cassiamin C having a therapeutic effect. Dihydrocapsaicin was identified at RT 7.572 and the Chlorophytum borivilianum root chloroform methanolic extract fraction noticeably depleted increased blood glucose levels and had positive effects on altered lipid profile after administering a dose of 150 mg/kg orally compared with oral hypoglycemic drug metformin. All the results are dose-dependent. Active chloroform-methanol fraction from methanol extract showed the presence of anti-diabetic compound, Dihydrocapsaicin. The chloroform-methanol fraction from the methanolic extract of Chlorophytum borivilianum root can inhibit the parameters linked to diabetes and hyperlipidemia.

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Fileti, Eudes E., and Sylvio Canuto. "Calculated infrared spectra of hydrogen-bonded methanol-water, water-methanol, and methanol-methanol complexes." International Journal of Quantum Chemistry 104, no. 5 (2005): 808–15. http://dx.doi.org/10.1002/qua.20585.

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Ramalingum, Nelvana, and M. Fawzi Mahomoodally. "Biologic Propensities and Phytochemical Profile ofVangueria madagascariensisJ. F. Gmelin (Rubiaceae): An Underutilized Native Medicinal Food Plant from Africa." BioMed Research International 2014 (2014): 1–15. http://dx.doi.org/10.1155/2014/681073.

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Vangueria madagascariensis(VM), consumed for its sweet-sour fruits, is used as a biomedicine for the management of diabetes and bacterial infections in Africa. The study aims to assess the potential of VM onα-amylase,α-glucosidase, glucose movement, and antimicrobial activity. The antioxidant properties were determined by measuring the FRAP, iron chelating activity, and abilities to scavenge DPPH, HOCl,∙OH, and NO radicals. Leaf decoction, leaf methanol, and unripe fruit methanol extracts were observed to significantly inhibitα-amylase. Active extracts againstα-glucosidase were unripe fruit methanol, unripe fruit decoction, leaf decoction, and ripe fruit methanol, which were significantly lower than acarbose. Kinetic studies revealed a mixed noncompetitive type of inhibition. Leaf methanolic extract was active againstS. aureusandE. coli. Total phenolic content showed a strong significant positive correlation (r=0.88) with FRAP. Methanolic leaf extract showed a more efficient NO scavenging potential and was significantly lower than ascorbic acid. Concerning∙OH-mediated DNA degradation, only the methanol extracts of leaf, unripe fruit, and ripe fruit had IC50values which were significantly lower thanα-tocopherol. Given the dearth of information on the biologic propensities of VM, this study has established valuable primary information which has opened new perspectives for further pharmacological research.

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Mathaiyan, Manikandan, Suriyavathana Muthukrishnan, Sobiya Pradeep, and Thamaraiselvi Ganesan. "Antioxidant status anticancer and hepato protective efficacy of Macrotyloma uniflorum seeds an In-vitro approach." International Journal of Zoology and Applied Biosciences 8, no. 1 (January 2023): 8–19. http://dx.doi.org/10.55126/ijzab.2023.v08.i01.002.

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The Macrotyloma uniflorum seed (Horse gram) has been underutilized pulse around the world. Nevertheless, it contains most valuable nutritional and pharmacological phytochemicals. Thus, this research was performed to evaluate the antioxidant, anticancer, and hepatoprotective potential of horse gram methanol extract. Interestingly, methanol extract of horse gram demonstrated dose dependent antioxidant, anticancer, and hepatoprotective activities. Since, at increased (100 mg mL-1) concentration the methanolic extract scavenged the DPPH radical up to 93.5% and reduced the ferric (Fe3+) into ferrous (Fe2+) up to 95%. Similarly, the methanol extract also possess considerable anticancer activities up to 75.64% at 300 mg mL-1 concentration. Furthermore, at increased concentration (300 mg mL-1), the methanol extract effectively protect (hepatoprotective) the HEK293 cells (95.71%), which exposed to 100 mM glucose (hepatotoxicity). The obtained results conclude that the horse gram methanol extract contains pharmacologically valuable phytochemicals and that regular intake of horse gram can be recommended to improve human health

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Hassan, Syeda Mona, Memmuna Hafeez, Syed Khurram Hassan, Asif Ibrahim, Huma Hassan, and Naureen Naeem. "Phytochemical Evaluation of A. esculentus Extracts." Lahore Garrison University Journal of Life Sciences 3, no. 1 (April 22, 2020): 1–9. http://dx.doi.org/10.54692/lgujls.2019.030150.

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The bioactives of A. esculentus leaves and seeds were extracted by using four solvent systems (80% methanol, 80% ethanol, 100% methanol and 100% ethanol) and leaves presented maximum extract yield (38.1 g/100g DW) in 80% methanolic solvent system. Antioxidant analysis of A. esculentus leaves and seeds extracts performed in terms of total phenolic and total flavonoid contents, showed that 80% methanolic leaves extract offered highest total phenolic contents (31.2 mg GAE/g DW), whereas 80% ethanolic leaves gave maximum total flavonoid contents (41.8 mg CE/g DW).

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Park, Hyuk, Young T. Ro, and Young M. Kim. "MdoR Is a Novel Positive Transcriptional Regulator for the Oxidation of Methanol in Mycobacterium sp. Strain JC1." Journal of Bacteriology 193, no. 22 (September 9, 2011): 6288–94. http://dx.doi.org/10.1128/jb.05649-11.

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Mycobacteriumsp. strain JC1 is able to grow on methanol as a sole source of carbon and energy using methanol:N,N′-dimethyl-4-nitrosoaniline oxidoreductase (MDO) as a key enzyme for methanol oxidation. The second open reading frame (mdoR) upstream of, and running divergently from, themdogene was identified as a gene for a TetR family transcriptional regulator. The N-terminal region of MdoR contained a helix-turn-helix DNA-binding motif. An electrophoretic mobility shift assay (EMSA) indicated that MdoR could bind to amdopromoter region containing an inverted repeat. ThemdoRdeletion mutant did not grow on methanol, but growth on methanol was restored by a plasmid containing an intactmdoRgene. In DNase I footprinting and EMSA experiments, MdoR bound to two inverted repeats in the putativemdoRpromoter region. Reverse transcription-PCR indicated that themdoRgene was transcribed only in cells growing on methanol, whereas β-galactosidase assays showed that themdoRpromoter was activated in the presence of methanol. These results indicate that MdoR serves as a transcriptional activator for the expression ofmdoand its own gene. Also, MdoR is the first discovered member of the TetR family of transcriptional regulators to be involved in the regulation of the methanol oxidation, as well as to function as a positive autoregulator.

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Gazali, Mohamad, Aden Nurin Fatimah, Amir Husni, Nurjanah Nurjanah, Zuriat Zuriat, and Rina Syafitri. "Antioxidant and Anti-Arthritic Activities of Green Seaweed Halimeda tuna Methanolic Extract." Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology 19, no. 1 (May 31, 2024): 45–54. http://dx.doi.org/10.15578/squalen.841.

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Seaweed contains various bioactive compounds rich in pharmacological potentials, such as antioxidants and anti-arthritic. This study aims to determine the antioxidant and anti-arthritic activities of the methanolic extract of Halimeda tuna. Halimeda tuna samples were extracted using the maceration method and methanol. The methanol extract was tested for antioxidant (2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP)) and anti-arthritic activity using denatured bovine serum albumin (BSA). The methanol extract was also subjected to phytochemical and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Research results revealed that the extract yield was 0.50 ± 0.06%. Phytochemical analysis showed that H. tuna methanolic extract contained flavonoids, steroids, and alkaloids. GC-MS analysis showed that H. tuna methanolic extract contained steroids, fatty alcohols, and fatty acids. The antioxidant activity tests revealed an IC50 value of 299.98 ± 121.15 ppm and a FRAP value of 200.02 ± 8.50 M/g, categorized as a weak antioxidant. Anti-arthritic activity of H. tuna methanolic extract showed an IC50 value of 877.29 ± 209.84 ppm. The anti-arthritic activity of H. tuna methanolic extract was lower than sodium diclofenac as a standard drug. The extract of H. tuna has antioxidant and anti-arthritic activities; hence, further exploration is needed as an alternative potential in the pharmacological field.

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Vale, Allister. "Methanol." Medicine 31, no. 10 (October 2003): 60–61. http://dx.doi.org/10.1383/medc.31.10.60.27825.

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Vale, Allister. "Methanol." Medicine 35, no. 12 (December 2007): 633–34. http://dx.doi.org/10.1016/j.mpmed.2007.09.014.

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Young, Jay A. "Methanol." Journal of Chemical Education 83, no. 8 (August 2006): 1131. http://dx.doi.org/10.1021/ed083p1131.

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Luttrell, William E., and Nicholas L. Conley. "Methanol." Journal of Chemical Health and Safety 18, no. 5 (September 2011): 56–58. http://dx.doi.org/10.1016/j.jchas.2011.07.007.

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Von Burg, R. "Methanol." Journal of Applied Toxicology 14, no. 4 (July 1994): 309–13. http://dx.doi.org/10.1002/jat.2550140412.

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Annesley, Thomas M. "Methanol-Associated Matrix Effects in Electrospray Ionization Tandem Mass Spectrometry." Clinical Chemistry 53, no. 10 (October 1, 2007): 1827–34. http://dx.doi.org/10.1373/clinchem.2007.090811.

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Abstract Background: Matrix effects can profoundly reduce the performance of electrospray ionization mass spectrometry. Preliminary observations indicated that the methanol used in the mobile phase could be a source of differential ionization or ion suppression. Methods: Drug stability studies, analysis of biological extracts, mixing experiments, and postcolumn infusions were used to test 9 commercial methanols for ionization differences in liquid chromatography-tandem mass spectrometry assays for immunosuppressants. Area responses for the drugs and internal standards were compared for mobile phases prepared with each selected methanol. Postcolumn infusion experiments were performed to confirm the degree of ionization differences occurring at the ion source, and to evaluate the proportions of ammonium, sodium, and potassium adducts. Results: The decrease in signal for the immunosuppressant drugs was shown to result from differential ionization associated with the selected methanols. Product ion intensity varied by 10-fold among the methanols tested. For sirolimus, tacrolimus, and mycophenolic acid, the percentage change in ionization was the same for the drug and its corresponding internal standard. Postcolumn sirolimus infusion evaluation revealed that a 1000-fold analyte concentration difference did not affect ionization. The proportions of ammonium, sodium, and potassium adducts of sirolimus precursor ions differed in relation to the source of methanol. Conclusions: Organic solvents used in mobile phases and extract preparation of biological samples may be associated with ion suppression, affecting adduct formation and assay sensitivity.

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I., Sulaikal Beevi, and A. Palavesam. "Bioactive Metabolites Profile of Methanol Flower and Seed Extracts of Clitoria ternatea (L.)." UTTAR PRADESH JOURNAL OF ZOOLOGY 44, no. 20 (September 13, 2023): 39–68. http://dx.doi.org/10.56557/upjoz/2023/v44i203645.

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Since ancient times, plants and plant products were used against numerous diseases. In this context, Clitoria ternatea (C. ternatea) was used for the various treatments of infectious diseases as a therapeutic role containing various phytochemical, antibacterial and antioxidant properties. The methanol flower and seed extracts of C.ternatea were analysed for antibacterial activity against Helicobacter pylori (H. pylori) using the agar well diffusion method. However, the probe of the antibacterial activity in both the methanol flower and methanol seed showed more or less the same zone of inhibition at 200 µg/ml. Furthermore, antioxidant properties were analysed by DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging activity and reducing power assay. Results on the DPPH assay showed better results in the methanol flower (42.79±0.0819) extract than methanol seed extract (37.41±0.0265) 200µg/ml. Likewise, the reducing assay manifested in the extract of methanol flower (0.90737±0.00375) was supremacy. Moreover the High resolution liquid chromatography-mass spectrometry (HRLCMS) analysis of methanol flower and seed extract of C. ternatea contained 32 and 51 major bioactive compounds, respectively in positive and negative modes. In light of the study, the extracts of methanol flower and seed extract of C. ternatea are utilized in the mode of action against H. pylori. The methanolic flower and seed extracts authenticated the presence of extensive identified and unidentified phytochemicals in C. ternatea and through more light on the important bioactive compounds to be explored for medicinal applications in future research.

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Ansari, Mojabir Hussen, and Sheelpriya R. Walde. "Phytochemical Extraction and Activity against Salmonella Typhi of Selaginella Bryopteris." International Journal of Medical & Pharmaceutical Sciences 13, no. 02 (2023): 01–03. http://dx.doi.org/10.31782/ijmps.2023.13201.

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Selaginella Bryopteris is widely known medicinal plant in India. It has many medicinal use such as anti-bacterial, antifungal and anti-cancer activity. The present study is performed to determine the activity of Selaginella Bryopteris belongs to family Selaginellaceae against Salmonella Typhi (Non-pathogenic strain). The activity is done by using methanolic extract of Selaginella Bryopteris against standard strain, Salmonella Typhi (Non-pathogenic strain). The testing is done by agar cup plate method. The zone of inhibition of methanolic extract of Selaginella Bryopteris is compared with methanol blank as a standard. The 50mg/ml methanolic extract of Selaginella Bryopteris shows maximum inhibition (7mm) whereas the blank methanol does not show any kind of inhibition.

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Buchanan, B. R., and D. E. Honigs. "Detection of Methanol in Gasolines Using Near-Infrared Spectroscopy and an Optical Fiber." Applied Spectroscopy 41, no. 8 (November 1987): 1388–92. http://dx.doi.org/10.1366/0003702874447077.

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Because of methanol's relatively low cost and its utility as an octane booster, a need to monitor the concentration of methanol in gasolines has evolved. Near-infrared analysis can be used to monitor methanol in gasolines with the use of optical fiber. If stepwise multiple linear regression is used, methanol can be predicted to within 0.25 weight percent. The use of an optical fiber lowers personnel and instrument exposure to hazardous environments and minimizes the risk of explosion. It is pointed out, however, that care must be taken in the prediction of future specimens. The differences between enumerative and analytical calibrations are also discussed.

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Khan, Arif, Rahmat Ali Khan, Mushtaq Ahmed, and Nadia Mushtaq. "In vitro antioxidant, antifungal and cytotoxic activity of methanolic extract of Calligonum polygonoides." Bangladesh Journal of Pharmacology 10, no. 2 (April 10, 2015): 316. http://dx.doi.org/10.3329/bjp.v10i2.22448.

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Present study is aimed at the pharmacological characterization of methanol extract of Calligonum polygonoides from District Bannu. Dried plant was grounded and extracted with methanol to prepare methanol crud extract. In vitro biological assays were conducted using this methanolic extract according to standard protocol. Cytotoxic activity of plant methanolic extract against brine shrimps while antifungal activity was also measured. Eighty percent death rate of brine shrimp was observed at 1,000 μg/mL of plant extract. 70 ± 0.0% growth inhibition of Aspergillus niger was measured during the present study. Significant scavenging results were observed during scavenging of free radicles viz; 78.1% against DPPH, 83.1% to ABTS and 36% against superoxide at 500 μg/mL were obtained. The results obtained in this study indicate that C. polygonoides possess significant antioxidant, antifungal and cytotoxic bioactive compounds.

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P, Selvaraj, Christina Angelin P, and Princy Rathnamala Jayaseeli J. "INSECTICIDAL AND ANTIBACTERIAL POTENTIAL OF SYZYGIUM AROMATICUM (L.) MERRILL AND PERRY." Journal of Biopesticides 12, no. 02 (December 1, 2019): 191–96. http://dx.doi.org/10.57182/jbiopestic.12.2.191-196.

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The present study was designed to appraise the insecticidal and antibacterial potential of Clove, Syzygium aromaticum (L.) against some laboratory hosts and Escherichia coli respectively. The phytochemicals constituents were extracted with two solvents methanol and acetone. The result of the phytochemical analysis revealed that methanol extract has alkaloids, steroids, tannins, saponins, flavonids, cardiac glycosides, phenolic compounds and aromatic acids but in acetone extract all the constituents are similar as methanolic extract except aromatic acids. Results of the repellent activity observed against Tribolium castaneum and Corcyra cephalonica implies that silver based nanoparticles from methanol extract of 1% concentration showed maximum repellent activity of 71% against T.castaneum. Antibacterial efficacy of clove extracts tested against Gram negative bacterium E. coli revealed that 1% of methanolic extract of clove is found to have maximum growth inhibition (25mm). Results of the larvicidal activity tested against Bombyx mori recorded 100% mortality in 4% acetone extracts of clove. Keywords: Syzygium aromaticum, Tribolium castaneum, Corcyra cephalonica, Bombyx mori, Repellent activity, Larvicidal ac

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Arabestani, Bita, Laleh Babaeekhou, and Maryam Ghane. "Antibacterial Activity of Different Artemisia dracunculus Extracts Against Dental Caries-Related Pathogens." Avicenna Journal of Dental Research 15, no. 3 (September 30, 2023): 87–91. http://dx.doi.org/10.34172/ajdr.492.

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Background: This study investigated the antibacterial properties of four types of tarragon (Artemisia dracunculus) extracts against two caries contributing bacteria, namely, Streptococcus mutans and Streptococcus sobrinus. Methods: The extracts of A. dracunculus were prepared using n-hexane, ethyl acetate, methanol, and water solvents. In addition, the diameter of inhibition zones, minimum inhibitory concentrations (MICs), and minimum bactericidal concentrations (MBCs) were determined, and finally, MTT (tetrazolium-based colorimetric assay) was used to analyze the cytotoxic effects of the extracts. Results: The well-diffusion method showed the antibacterial property of four tested extracts against bacteria. Methanol and water extracts made the highest inhibition zone diameters (P<0.001). This was true for both tested bacteria. The mIC of the methanol, water, n-hexane, and ethyl acetate extracts were 0.78, 1.5, 3.1, and 1.5 mg/mL, as well as 0.78, 0.78, 1.5, and 1.5 mg/mL against S. mutans and S. sobrinus, respectively. The half-maximal inhibitory concentration (IC50) values for methanolic, aqueous, ethyl acetate, and n-hexane extracts were 0.78, 0.78, 1.56, and 3.12 mg/mL, respectively. Conclusions: The results provided a rational reason for the traditional use of A. dracunculus extracts against anti-caries-related bacteria. The methanolic extract demonstrated better activity, thus methanol can probably extract a wider range of plant compounds with antibacterial effects.

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More, Keshav Kalyan, Rajendra Maratrao Gade, Nitin Vitthal Gurav, and Rupeshkumar Jagannath Chaudhari. "Phytochemical Investigation and Thin Layer Chromatography of Methanol Extract of Psoralea corylifolia and Emblica officinalis Leaves." International Journal of Plant & Soil Science 35, no. 19 (August 24, 2023): 653–63. http://dx.doi.org/10.9734/ijpss/2023/v35i193595.

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The current study focuses on phytochemical screening of Psoralea corylifolia and Emblica officinalis leaf extracts in methanol solvent. The antibacterial compounds found in both leaf extracts of Psoralea corylifolia and Emblica officinalis plants were investigated using phytochemical studies. The extracts contained flavonoids, terpenoids, tannins, alkaloids, saponins, and phenolic chemicals, according to preliminary phytochemical screening. The solvent systems of toluene: ethyl acetate: methanol (24:5:2) and chloroform: ethyl acetate: acetic acid (50:50:1) yielded the most phytochemicals from methanolic extracts of Psoralea corylifolia and Emblica officinalis plants, respectively. On TLC plates, these chemicals were separated, resulting in the discovery of different spots in both leaf extracts. The Rf values of methanol extract of P. corylifolia run under toluene: ethyl acetate: methanol (24:5:2) solvent system was 0.12, 0.19, 0.30, 0.41, 0.53, 0.65, 0.77, 0.84, 0.89, and 0.92, respectively, while Rf values of methanol leaf extract of E. officinalis run under chloroform: ethyl acetate: acetic acid (50:50:1)solvent system was 0.23, 0.31, 0.41, 0.64, 0.76 and 0.88 respectively. The results of the investigation will be used to confirm the proper identification of antibacterial fractions from P. corylifolia and E. officinalis crude plant extracts by GC-MS. The optimum solvent for extracting antibacterial components from P. corylifolia and E. officinalis leaves was methanol.

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Maharramova, L. G. "CONVERSION OF METHANOL TO DIMETHOXYMETHANE." Azerbaijan Chemical Journal, no. 4 (December 12, 2019): 65–69. http://dx.doi.org/10.32737/0005-2531-2019-4-65-69.

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Lu, Xiaoqing, Zhigang Deng, Chen Guo, Weili Wang, Shuxian Wei, Siu-Pang Ng, Xiangfeng Chen, Ning Ding, Wenyue Guo, and Chi-Man Lawrence Wu. "Methanol Oxidation on Pt3Sn(111) for Direct Methanol Fuel Cells: Methanol Decomposition." ACS Applied Materials & Interfaces 8, no. 19 (May 4, 2016): 12194–204. http://dx.doi.org/10.1021/acsami.6b02932.

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Hernández-Fernández, Adrián, Yolanda Garrido, Eduardo Iniesta-López, Antonia Pérez de los Ríos, Joaquín Quesada-Medina, and Francisco José Hernández-Fernández. "Recovering Polyphenols in Aqueous Solutions from Olive Mill Wastewater and Olive Leaf for Biological Applications." Processes 11, no. 9 (September 6, 2023): 2668. http://dx.doi.org/10.3390/pr11092668.

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Olive leaf and olive mill wastewater are wastes which are produced in olive industry and can be used to obtain value-added products such as polyphenols. In this work, extracts of polyphenols from olive mill wastewater and olive leaf in an aqueous medium are obtained and their composition in polyphenols were determined and compared with those obtained using the conventional extract agent (methanol–water). The antioxidant capacity of the aqueous extracts of olive mill wastewater and olive leaf were also analysed. It was observed that the olive mill wastewater extracted in water provides a similar content of phenolic compounds in the final extract compared to extraction with methanol–water, with the main polyphenols being hydrotyrosol and tyrosol (20.1 and 6.61 mg gdw−1). In the case of leaf extraction, the methanol-free extract (recovery in water after extraction with methanol–water) had a lower total phenol content compared to the methanolic extract, with the main polyphenol being oleuropein (22.73 and 9.05 mg gdw−1, for the methanol and methanol-free extract, respectively). However, both extracts obtained in aqueous solution present a similar antioxidant capacity at very diluted concentrations of the original extract, with IC50 values (half-maximal antioxidant concentration) of about 20 mg TS L−1. The antioxidant capacity of the extract in aqueous solution facilitates its application as an antioxidant in biological systems, like animal food, where the use of extracts based on organic solvents, like methanol, are not suitable.

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Royani, Ahmad, Muhammad Hanafi, Heddy Julistiono, Achmad Dinoto, Puspa Dewi N. Lotulung, and Azwar Manaf. "The Potential of Tinospora cordifolia Extracts as Antibacterial Material against Pseudomonas aeruginosa." Trends in Sciences 20, no. 1 (November 28, 2022): 3884. http://dx.doi.org/10.48048/tis.2023.3884.

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Mitigation and control of bacterial corrosion must have been prioritized for failure anticipation by corrosion associated with microorganisms. Using synthetic inhibitor for material protection from corrosion have problems because it is not eco-friendly. This work conducted preliminary studies on T. cordifolia stem extracts at various methanol ratios against P. aeruginosa biofilm as a new eco-friendly inhibitor. The T. cordifolia stem was extracted by the maceration method with a different ratio of methanol solvent (100, 75 and 50 %). The bacterial activity was assessed using the dilution method (MTT assay) to determine the minimum inhibitory concentration (MIC). The total phenolic and flavonoid contents were determined using Follin-Ciocalteu and aluminum chloride (AlCl3) colorimetric, respectively. Meanwhile, the structure of the active compounds in the extract was identified by using the liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The yields of T. cordifolia extracts are 9.45, 7.56 and 8.40 % at 100, 75 and 50 % of methanol ratios, respectively. The total phenolic content (TPC) in crude methanol extract of T. cordifolia is 11.20, 9.46 and 6.56 % for 100, 75 and 50 % of methanol ratios. Meanwhile, the flavonoid content (TFC) was obtained is about 5.25, 0.64 and 0.33 % for 100, 75 and 50 % of methanol concentrations, respectively. The T. cordifolia extract has antibacterial activity against P. aeruginosa in all ratios of methanol concentrations. The minimum inhibitory concentration (MIC) of T. cordifolia methanol extract was found at 4096 µg/mL. Methanol solvent concentration with the most active antibacterial activity of the extract was 50 %, followed by 75 and 100 %. The structures of the active compound in the methanolic extract of T. cordifolia are a phenolic group, and it is in the alkaloid derivatives (Calopiptin, d-Lirioferine (Lirioferine), Moupinamide, Piperanine, and Yuanhunine). HIGHLIGHTS The crude methanol extract of Tinospora cordifolia stem has potential against aeruginosa in the marine environment The minimum inhibitory concentration (MIC) was found at 4096 µg/mL of extract in a ratio of 50:50 (v/v) of methanol to water The compound structures in cordifolia methanol extracts are Calopiptin, d-Lirioferine (Lirioferine), Moupinamide, Piperanine, and Yuanhunine GRAPHICAL ABSTRACT

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Akakpo, Christian, John Kenneth Mensah, and Clement Osei Akoto. "Antimicrobial, antioxidant, anthelmintic and anti-inflammatory bioactivities of Sabicea calycina." International Journal of Biological and Chemical Sciences 16, no. 3 (August 27, 2022): 1084–99. http://dx.doi.org/10.4314/ijbcs.v16i3.15.

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Sabicea calycina is broadly used for the treatment of different diseases including sexually transmitted infection, gastrointestinal disorders, wound treatment, and wound dressing. This study is focused on assessing the biological activity (anthelmintic, antioxidant, antimicrobial, and anti-inflammatory) studies of methanol and hexane leaf crude extracts of S. calycina on its ethno medicinal uses. Phytochemical analyses revealed the presence of six phytoconstituents in the methanolic extract and by demonstrating four in the hexane extract. In vitro, the anthelmintic activity of the crude extracts was examined against Milsonia ghanensis (Earthworms) using albendazole as the standard drug. The anthelmintic activity of methanol and hexane extracts at test concentrations was observed to be significantly higher compared to albendazole. The methanolic extract was comparatively the more potent antioxidant as ascertained by its relatively higher antioxidant capacity TAC and its disproportionately lower for the DPPH and H2O2. Regularly for each of the group of four bacterial and one fungal pathogenic microbial cell lines, the methanolic extract showed higher antimicrobial activity values, MICs that were quantitatively lower than that of the hexane extract. In vivo anti-inflammatory activity of methanol and hexane extracts was assessed against diclofenac as a reference drug, utilizing the carrageenan-induced chick foot edema method using 7-day old chicks. This method indicated the methanol extract showing a dose- dependent decreasing in foot edema and the hexane extract lack of showing measurable anti-inflammatory activity. The results show that the extracts could be employed as a remedial contender for the treatment of helminthic, oxidation, microbial and inflammatory diseases.

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Su, Ay, Ying Chieh Liu, Wei Chieh Lin, Chih Kai Cheng, and Jai Houng Leu. "Integration Study of Micro Reformer and High Temperature PEM Fuel Cell." Advanced Materials Research 197-198 (February 2011): 730–35. http://dx.doi.org/10.4028/www.scientific.net/amr.197-198.730.

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An integration platform assembling with methanol reformer and high-temperature proton exchange membrane fuel cell (PEMFC) was constructed in this present. The methanol micro reformer combines with catalytic reaction section and reforming section. Catalytic reaction section with Pt calalysis maintains the constant temperature envoriment for reforming process. SRM reforming results show the 74%~74.9% hydrogen and 23.5%~25.7% of carbon dioxide in the mixture product. Less than 2% of carbon monoxide was produced. Using the reforming product of low carbon monoxide concentration and the highest methanol conversion rate, a micro reformer links with fuel cell integration experiment was performed. Results show the high temperature PEMFC with 3 ~ 4W power output under methaol flow rate 15ml/hr. Due to the lower of hydrogen pressure supplying from the micro reformer, may cause the fuel cell power output become unstable.

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Bashyal, Sagar, and Avijit Guha. "EVALUATION OF TRACHYSPERMUM AMMI SEEDS FOR ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS." Asian Journal of Pharmaceutical and Clinical Research 11, no. 5 (May 1, 2018): 274. http://dx.doi.org/10.22159/ajpcr.2018.v11i5.24430.

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Objective: The objective of this study is to identify medicinally important phytochemicals and evaluate the antimicrobial potential of Trachyspermum ammi seeds. Methods: Four different extracts (methanol, acetone, chloroform, and water) were prepared using a soxhlet apparatus, antimicrobial activity was tested using agar well-diffusion technique. Results: The results revealed the presence of flavonoids and saponins in all the extracts prepared. Similarly, alkaloids and phenols presence were obtained in methanol and aqueous extracts. Glycosides and carbohydrates in methanol, chloroform, and aqueous extracts. Further, proteins, terpenoids, and tannins presence were found in methanol, chloroform, and aqueous extracts, respectively. The maximum zone of inhibition was found in the methanolic extract (13.5 mm). Acetone, chloroform, and water extracts showed 9 mm, 10.5 mm, 11 mm respectively, while ciprofloxacin (control) showed 17.5 mm of the zone of inhibition. Conclusion: T. ammi seeds exert biological properties due to the presence of various chemical constituents. Thus, it can be used to obtain novel antibacterial compounds for the treatment of infectious diseases in the future.

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Berkowicz, Gabriela, Witold Żukowski, and Jerzy Baron. "Oxidative decomposition of methanol in a vibroacoustic fluidized bed of Ag-coated cenosphere core-shell catalyst." Polish Journal of Chemical Technology 18, no. 4 (December 1, 2016): 71–75. http://dx.doi.org/10.1515/pjct-2016-0073.

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Abstract The paper presents results of a study of oxidative decomposition of methanol in a fluidized bed of silver catalyst. The process of methanol oxidation was carried out on Ag-coated cenospheres core-shell catalyst. The catalyst was obtained by precipitation of silver from methanolic solution of silver nitrate on cenospheres. Cenospheres are lightweight, inert, hollow spheres, which can be easily introduced into a fluidized bed. Application of the catalyst in a form of fluidized bed should ensure good temperature equalization which is very important at low temperatures due to the possibility of generation of formaldehyde. It turned out that local hot points occur in that kind of a catalyst (in the form of a fluidized bed but with very low density), thus use of additional acoustic wave is necessary to ensure good control over the temperature of the process. The products of the process of methanol oxidation were monitored on-line by Fourier transform infrared spectroscopy (FTIR). The catalyst has proven to be highly active in the oxidative decomposition of methanol. Full of methanol conversion in reaction of complete oxidation was achieved at 350°C.

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Sugihartini, Nining, Mohamad Usman Nur, and Sapto Yuliani. "The Effect of Methanol Concentration on the Extraction of Moringa Leaf (Moringa oleifera) and Papaya Fruit (Carica papaya) on Elastase and Hyaluronidase Installing Activities." Open Access Macedonian Journal of Medical Sciences 10, A (September 3, 2022): 1463–70. http://dx.doi.org/10.3889/oamjms.2022.10302.

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Abstract BACKGROUND OF THE STUDY: Β-carotene, flavonoids and phenolic compounds found in methanol extracts of Moringa leaves and papaya fruit have high antioxidant activity so that they can be used for antiaging. The concentration of solvent is a factor that will affect the levels of active ingredients in methanol extract. This study aims to determine the effect of the concentration of the methanol solvent derived from the extraction of Moringa leaves and papaya fruit on the inhibitory activity of the elastase and hyaluronidase enzymes. AIM OF THE STUDY: Knowing the best extracts of methanol 50, 70, and 96% of Moringa leaf (Moringa oleifera) and papaya fruit (Carica papaya) as antiaging agents through inhibition of elastase and hyaluronidase enzymes. METHODOLOGY: In this study variations in the concentration of methanol 50%, 70%, and 96% were used for the extraction of Moringa leaves and papaya fruit. The extract was obtained by maceration method which was then tested for the inhibition of the enzyme activity of elastase and hyaluronidase using ELISA (Enzyme-Linked Immunosorbent Assay). RESULTS: The results of the elastase enzyme inhibitory activity test on Moringa leaf extract with 50%, 70%, and 96% methanol solvents IC50 respectively = 9453.38; 6604.70; 12346.44 μg / mL, while the yield of papaya extract 5995.31; 9046.25; 11571.54 μg / mL. In addition, the test results showed the inhibitory activity of the hyaluronidase enzyme showed that the Moringa leaf extract with 50%, 70%, and 96% solvents IC50 respectively = 2944.53; 1028,36; 3001.83 μg / mL, while the yield of methanol extract of papaya fruit is 982.67; 2982.96; 3530.18 μg / mL. CONCLUSION: Based on the test results it can be concluded that the most effective solvent concentration as an inhibition of the enzyme elastase and hyaluronidase methanolic extract of Moringa leaves is 70% methanol solvent. While papaya fruit methanol extract which is effective in inhibiting the enzymes elastase and hyaluronidase is 50% methanol solvent.

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Zhao, Fei, XiShang Sun, Ruifeng Lu, and Lihua Kang. "Adsorption of methanol, methanal, toluene, ethylbenzene, and styrene in zeolites: a grand canonical Monte Carlo simulation study." Canadian Journal of Chemistry 95, no. 12 (December 2017): 1241–47. http://dx.doi.org/10.1139/cjc-2017-0218.

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In this study, the adsorption behaviors of methanol, methanal, toluene, ethylbenzene, and styrene molecules in FAU, FER, CON, and MWW zeolites were investigated. The adsorption isotherms of the five adsorbates in the four zeolites at 298 and 350 K were simulated using grand canonical Monte Carlo simulations. Moreover, binary component adsorptions were considered. The results revealed that the saturated adsorption capacity of single components in different zeolites decreased in the order of FAU > MWW > CON > FER, and the adsorption capacity of the five adsorbates in the same zeolite decreased in the order of methanal > methanol > toluene > styrene > ethylbenzene. The equilibrium adsorption capacity slightly decreased with increasing temperature. In terms of binary component adsorption, intense competition existed between the smaller adsorbed molecules. As the differences among the molecular structures increased, the competition in adsorption became more intense.

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Paik, Younkee, Seong-Soo Kim, and Oc Hee Han. "Methanol Behavior in Direct Methanol Fuel Cells." Angewandte Chemie International Edition 47, no. 1 (January 2008): 94–96. http://dx.doi.org/10.1002/anie.200703190.

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Thrane, Joachim, Sebastian Kuld, Niels D. Nielsen, Anker D. Jensen, Jens Sehested, and Jakob M. Christensen. "Methanol‐Assisted Autocatalysis in Catalytic Methanol Synthesis." Angewandte Chemie 132, no. 41 (August 13, 2020): 18346–50. http://dx.doi.org/10.1002/ange.202006921.

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Thrane, Joachim, Sebastian Kuld, Niels D. Nielsen, Anker D. Jensen, Jens Sehested, and Jakob M. Christensen. "Methanol‐Assisted Autocatalysis in Catalytic Methanol Synthesis." Angewandte Chemie International Edition 59, no. 41 (August 13, 2020): 18189–93. http://dx.doi.org/10.1002/anie.202006921.

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Paik, Younkee, Seong-Soo Kim, and Oc Hee Han. "Methanol Behavior in Direct Methanol Fuel Cells." Angewandte Chemie 120, no. 1 (January 2008): 100–102. http://dx.doi.org/10.1002/ange.200703190.

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Alawode, T. T., L. Lajide, B. J. Owolabi, and M. T. Olaleye. "Investigation of Bulb Extracts of Crinum jagus for Antibacterial and Antifungal Activities." Journal of Applied Sciences and Environmental Management 25, no. 1 (March 31, 2021): 113–17. http://dx.doi.org/10.4314/jasem.v25i1.16.

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This study investigates the bulb extracts of Crinum jagus for antimicrobial activities. The bulb samples were dried, ground and subjected to successive extraction using hexane, ethylacetate and methanol. The extracts were screened for activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhi, Klebisidlae pneumonae, Candida albicans, Aspergillus niger, Penicillium notatum and Rhizopus stolonifer at concentrations between 6.25 and 200 mg/ml. Antimicrobial assays werecarried out using agar diffusion method. The Minimum Inhibitory Concentration (MIC) of the extracts was determined. The percentage yields obtained for the hexane, ethylacetate and methanol extracts of the bulbs are 0.28 %, 0.44% and 24.68 % respectively. Results showed that the methanolic extract had better antibacterial and antifungal activities than the other extracts. The methanol extract showed the highest antibacterial activity against S. aureus with a zone of inhibition of 28 mm at 200 mg/ml. Also, the methanol extract showed the highest antifungal activities against C. albicans, A. niger and P. notatum with a zone of inhibition of 20 mm at 200 mg/ml. The hexane and ethylacetate extracts had no activity against the microorganisms at 6.25 mg/ml.The methanol extract had the lowest Minimum Inhibitory Concentration (MIC) of 2.50 mg/ml against S. aureus, E. coli, B. subtilis, P. aeruginosa and S. typhi. The methanol extract could be a source of potent antimicrobial compounds. Keywords: Antibacterial, Antifungal, Crinum jagus, Agar diffusion, MIC

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Khan, Arif, Arvind Singh Jadon, and Poonam Bhadauriya. "Evaluation of Extractive Value and In-Vitro antimicrobial potential of Curcuma longa using disk diffusion method." Journal of Drug Delivery and Therapeutics 12, no. 6-S (December 15, 2022): 30–35. http://dx.doi.org/10.22270/jddt.v12i6-s.5842.

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The efficiency of turmeric fractions, such as petroleum ether, chloroform, benzene, methanol and aqueous were evaluated for their inhibitory effect on clinical and standard strains of pathogenic bacteria and fungus. The methanolic fraction of Curcuma longa rhizome had high potential to inhibit some pathogenic bacteria i.e. S. aureus to a greater degree than other fractions of Curcuma longa. In our study the results show that the different fractions (petroleum ether, methanol etc.) of Curcuma longa rhizome were more effective antimicrobial agents than the crude extract of Curcuma longa. Methanolic extract of curcuma longa was evaluated against gram positive and gram-negative bacteria. The bacteria isolates include Bacillus subtilis and Escherichia coli. Antimicrobial activities were estimated by Well diffusion method and antifungal activity was evaluated against Candida Albicans and Aspergillus Niger. The results were pointed as the methanol extract was more effective on both bacteria and fungus then water also similar effective on both bacteria and fungus but against Aspergillus Nigerit has outstand results. Keywords: Curcuma longa, Phytochemical, Antimicrobial, Free radical, Scavenging Potential

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Kokkaiah Irulandi, Sethupandian Geetha, Anantharaj Tamilselvan, and Palanichamy Mehalingam. "GC-MS analysis and phytochemical studies of methanolic fruits extract of Garcinia cambogia Hort. Ex Boerl and Ziziphus trinervia Roth." JOURNAL OF ADVANCED APPLIED SCIENTIFIC RESEARCH 1, no. 3 (December 15, 2021): 90–95. http://dx.doi.org/10.46947/joaasr13201623.

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The investigation was carried out to determine the possible phytochemical components from the methanolic extracts of Garcinia cambogia (fruits) and Ziziphus trinervia (fruits). Among the phytochemical screening of these two plant extracts showed that the plant was rich in alkaloids, flavonoids, phenols, saponins and quinones. This study was extended by analyzing the potent bioactive compounds in the methanolic extract of Garcinia cambogia (fruits) and Ziziphus trinervia (fruits) using GC-MS. The analysis revealed that Garcinia cambogia fruits extracts 40 compounds were identified in the fruit methanol extract. Another one plant Ziziphus trinervia methanol fruit extract showed 3 compounds were identified. Medicinal potential of these compounds needs further research on microbial aspects to develop safe drug

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Tirumala, Duguta, and V. Jayashree. "Isolation of Ethyl Acetate Fraction of Methanolic Extract of Cinnamomum malabatrum Leaves by Column Chromatography." INTERNATIONAL JOURNAL OF PHARMACEUTICAL QUALITY ASSURANCE 14, no. 04 (December 25, 2023): 888–92. http://dx.doi.org/10.25258/ijpqa.14.4.11.

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Cinnamomum malabatrum (Lauraceae) is available in the Western Ghats and south India, the leaves were collected shade dried and made into fine powder form, and the powder was subjected to extraction by different solvents like petroleum ether, methanol, and water. Further, these extracts were subjected to thin layer chromatography. C. malabatrum ethyl acetate fraction of methanolic extract was given strong evidence for active components, based on evidence ethyl acetate fraction was subjected for further chromatographic separation; column chromatography was used for isolation of component of C. malabatrum ethyl acetate fraction of methanolic extract was subjected to column chromatography by using different ratios of mobile phases (in Hexane and Methanol). Flavonoids and phenols found present.

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Al-Nemi, Ruba, Arwa A. Makki, Khaled Sawalha, Dina Hajjar, and Mariusz Jaremko. "Untargeted Metabolomic Profiling and Antioxidant Capacities of Different Solvent Crude Extracts of Ephedra foeminea." Metabolites 12, no. 5 (May 17, 2022): 451. http://dx.doi.org/10.3390/metabo12050451.

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Ephedra foeminea is a traditional medicinal plant used in the Eastern Mediterranean region. This study aims to investigate the chemical profiles of different solvent extracts of E. foeminea via an untargeted metabolomics approach, alongside determining their antioxidant capacities. E. foeminea samples collected from Jordan were macerated in solvents of varying polarities; dichloromethane/methanol, methanol, ethanol, ethyl acetate, and acetone. The crude extracts were subjected to comprehensive chemical profiling and metabolomics study using Gas chromatography–Mass spectrometry (GC–MS), Liquid chromatography–Mass spectrometry (LC–MS), and Nuclear Magnetic Resonance (NMR). The obtained data were analyzed using Venn diagrams, Principle Component Analysis (PCA), and Metabolite Enrichment Set Analysis (MESA). ABTS assay was performed to measure the crude extracts’ antioxidant activity. MESA revealed the dominant chemical groups as amino acids, fatty acids, carboxylic acids, and carbohydrates. Results indicated that dichloromethane/methanol and methanolic extracts had the most distinct composition as well as the most unique compounds. The methanolic extract had the most potency (IC50 249.6 µg/mL) in the ABTS assay. However, no significant differences were found. In conclusion, solvents influenced the recovery of metabolites in E. foeminea and the antioxidant activity of the E. foeminea methanolic extract could be correlated to the abundant presence of diverse bioactive compounds.

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Ramasamy, Shanmuga Priya, Anitha Rajendran, Muthukrishnan Pallikondaperumal, Priya Sundararajan, Fohad Mabood Husain, Altaf Khan, Mohammed Jamal Hakeem, et al. "Broad-Spectrum Antimicrobial, Antioxidant, and Anticancer Studies of Leaf Extract of Simarouba glauca DC In Vitro." Antibiotics 11, no. 1 (January 3, 2022): 59. http://dx.doi.org/10.3390/antibiotics11010059.

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The current study aimed to screen the preliminary phytochemicals in the leaf extract of the medicinal plant Simarouba glauca and to analyze its potential antimicrobial, antioxidant and anticancer properties. The phytochemical profile of the methanol extract was analyzed, and bioactive compounds were identified using chromatography, FTIR and GCMS. Antimicrobial activity and Minimum Inhibitory Concentration (MIC) were determined against 14 bacterial and 6 fungal strains. Moreover, the synergistic effect of a plant extract with commercially available antibiotics was also evaluated using the checkerboard method. The ethanolic and methanolic extracts showed exclusive activity against S. aureus and profound activity against E. coli and S. marcescens. Upon comparing breakpoints, methanolic extract demonstrated higher antimicrobial activity with a MIC value of 3.2 mg/mL against the test pathogens. Furthermore, the extracts demonstrated potential antioxidant activity; methanol extract had higher antioxidant potential compared to the ethanol extract. The major proactive bioactive compound with maximum antioxidant capacity was observed to be terpenoids. The methanol extract of S. glauca showed significant cytotoxicity against the MCF-7 breast cancer cell line with an IC50 value of 16.12 µg/mL. The overall results of our work provide significant evidence for the usage of methanolic extract of S. glauca as an efficient ethnomedicinal agent and a potential candidate for relieving many human ailments.

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Journal articles on the topic 'Methanol'

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