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Journal articles on the topic 'Melanoma, Cancer, Tomato, Polyphenols'
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1
Pop, Teodora Daria, and Zorita Diaconeasa. "Recent Advances in Phenolic Metabolites and Skin Cancer." International Journal of Molecular Sciences 22, no. 18 (September 8, 2021): 9707. http://dx.doi.org/10.3390/ijms22189707.
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Skin cancer represents any tumor development from the cutaneous structures within the epidermis, dermis or subcutaneous tissue, and is considered to be the most prevalent type of cancer. Compared to other types of cancer, skin cancer is proven to have a positive growth rate of prevalence and mortality. There are available various treatments, including chemotherapy, immunotherapy, radiotherapy and targeted therapy, but because of the multidrug resistance development, a low success has been registered. By this, the importance of studying naturally occurring compounds that are both safe and effective in the chemoprevention of skin cancer is emphasized. This review focuses on melanoma because it is the deadliest form of skin cancer, with a significantly increasing incidence in the last decades. As chemopreventive agents, we present polyphenols and their antioxidant activity, anti-inflammatory effect, their ability to balance the cell cycle and to induce apoptosis and their various other effects on skin melanoma. Besides chemoprevention, studies suggest that polyphenols can have treating abilities in some conditions. The limitations of using polyphenols are also pointed out, which are related to their poor bioavailability and stability, but as the technology is well developed, it is possible to augment the efficacy of polyphenols in the case of melanoma.
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Isacescu, Ecaterina, Paul Chiroi, Oana Zanoaga, Andreea Nutu, Liviuta Budisan, Radu Pirlog, Atanas G. Atanasov, and Ioana Berindan-Neagoe. "Melanoma Cellular Signaling Transduction Pathways Targeted by Polyphenols Action Mechanisms." Antioxidants 12, no. 2 (February 7, 2023): 407. http://dx.doi.org/10.3390/antiox12020407.
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Melanoma is the most aggressive type of skin cancer. Although different anti-melanoma treatments are available, their efficacy is still improvable, and the number of deaths continues to increase worldwide. A promising source of antitumor agents could be presented by polyphenols—natural plant-based compounds. Over the past decades, many studies have described multiple anticancer effects of polyphenols in melanoma, presenting their potential interactions with targeted molecules from different signaling pathways. However, to our knowledge, there is no comprehensive review on polyphenols-regulated mechanisms in melanoma cells available in the literature. To fulfill this gap, this article aims to summarize the current knowledge of molecular mechanisms of action regulated by polyphenols involved in melanoma initiation and progression. Here, we focus on in vitro and in vivo effects of polyphenol treatments on tumor-essential cellular pathways, such as cell proliferation, apoptosis, autophagy, inflammation, angiogenesis, and metastasis. Moreover, emerging studies regarding the well-marked role of polyphenols in the regulation of microRNAs (miRNAs), highlighting their contribution to melanoma development, are also epitomized. Finally, we hope this review will provide a firm basis for developing polyphenol-based therapeutic agents in melanoma treatment.
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Martí, Raúl, Salvador Roselló, and Jaime Cebolla-Cornejo. "Tomato as a Source of Carotenoids and Polyphenols Targeted to Cancer Prevention." Cancers 8, no. 6 (June 20, 2016): 58. http://dx.doi.org/10.3390/cancers8060058.
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Chen, Xianjin, Lili Chang, Yan Qu, Jinning Liang, Waishu Jin, and Xiujuan Xia. "Tea polyphenols inhibit the proliferation, migration, and invasion of melanoma cells through the down-regulation of TLR4." International Journal of Immunopathology and Pharmacology 31 (January 1, 2018): 039463201773953. http://dx.doi.org/10.1177/0394632017739531.
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Melanoma is the most common skin cancer and malignant melanoma which can cause skin cancer-related deaths. Toll-like receptor 4 (TLR4) had been reported to play an important role in melanoma, and tea polyphenol (TP) is regarded as an anticancer substance. However, the relationship between TP and TLR4 in melanoma is not well explored. Therefore, our aim is to figure out how TP has an influence on melanoma. Melanoma cell lines (B16F10 and A375) were treated with TP and lipopolysaccharides (LPS). Western blot assay was used to examine TLR4 expression, and MTT assay was conducted to assess proliferation. Wound healing assay was conducted to evaluate the migration of melanoma cells, and transwell assay was used to examine the melanoma cells’ invasiveness. Besides, in vivo experiments were practiced for TP function in mice with melanoma cells. TP inhibited the proliferation, migration and invasion ability of melanoma cells, which displayed a dosage and time dependence. TLR4 was highly expressed in melanoma cells compared with normal skin cells. TP could suppress TLR4 expression both in normal melanomas and in stimulated melanomas by TLR4 agonist LPS. Suppressing TLR4 in melanomas could inhibit cell function (proliferation, migration, and invasion), and blocking the expression of 67LR could abolish TP function on TLR4. TP can inhibit melanoma (B16F10) growth in vivo.
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Helyes, Lajos, Zoltán Pék, Sára Brandt, and Andrea Lugasi. "Analysis of Antioxidant Compounds and Hydroxymethylfurfural in Processing Tomato Cultivars." HortTechnology 16, no. 4 (January 2006): 615–19. http://dx.doi.org/10.21273/horttech.16.4.0615.
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Tomato (Lycopersicon esculentum) fruit is an important source of antioxidants, which are important in the prevention of many cancer types and cardiovascular diseases. In ripe tomato fruit, one of the most significant antioxidant compounds is lycopene. In this study, effects of the cultivars and the environmental conditions on the lycopene content were investigated. Temperature effect was characterized by calculating growing degree-day (GDD). Relationships among lycopene, total polyphenols, organic acid, and total antioxidant status (TAS) were analyzed. A variation of more than 30% was found among the lycopene levels of five processing tomato cultivars. Lycopene content ranged from 79.7 to 155.0 mg·kg–1 fresh weight. There was a significant difference between the lycopene concentrations of fruit harvested at two different dates. The values were higher at the second harvest, probably due to the cooler weather conditions preceding that harvest. Total polyphenols correlated strongly with TAS but the other antioxidants did not have any relationship to TAS. GDD were strongly and positively correlated with hydroxymethylfurfural content in fresh tomatoes.
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Prakash, Monica D., Lily Stojanovska, Jack Feehan, Kulmira Nurgali, Elizabeth L. Donald, Magdalena Plebanski, Matthew Flavel, Barry Kitchen, and Vasso Apostolopoulos. "Anti-cancer effects of polyphenol-rich sugarcane extract." PLOS ONE 16, no. 3 (March 10, 2021): e0247492. http://dx.doi.org/10.1371/journal.pone.0247492.
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Plant polyphenols have an array of health benefits primarily thought to be related to their high content of anti-oxidants. These are commonly undervalued and knowledge of their biological properties have grown exponentially in the last decade. Polyphenol-rich sugarcane extract (PRSE), a natural extract from sugar cane, is marketed as high in anti-oxidants and polyphenols, but its anti-cancer activity has not been reported previously. We show that, PRSE exerts anti-cancer properties on a range of cancer cells including human (LIM2045) and mouse (MC38, CT26) colon cancer cells lines; human lung cancer (A549), human ovarian cancer (SKOV-3), pro-monocytic human leukemia (U937) and to mouse melanoma (B16) cell lines; whereas no effects were noted on human breast (ZR-75-1) and human colon (HT29) cancer cell lines, as well as to human normal colon epithelial cell line (T4056). Anti-proliferative effects were shown to be mediated via alteration in cytokines, VEGF-1 and NF-κB expression.
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Fortes, C., S. Mastroeni, F. Melchi, M. A. Pilla, G. Antonelli, D. Camaioni, M. Alotto, and P. Pasquini. "FC4 Foods rich in polyphenols and N-3 fatty acids decrease cutaneous melanoma risk." Melanoma Research 20 (June 2010): e30. http://dx.doi.org/10.1097/01.cmr.0000382806.14581.52.
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Obrador, Elena, Rosario Salvador-Palmer, Ali Jihad-Jebbar, Rafael López-Blanch, Thanh H. Dellinger, Ryan W. Dellinger, and José M. Estrela. "Pterostilbene in Cancer Therapy." Antioxidants 10, no. 3 (March 21, 2021): 492. http://dx.doi.org/10.3390/antiox10030492.
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Natural polyphenols are organic chemicals which contain phenol units in their structures and possess antitumor properties. However, a key problem is their short half-life and low bioavailability under in vivo conditions. Pterostilbene (3,5-dimethoxy-4′-hydroxystilbene; PT) is a phytoalexin originally isolated from the heartwood of red sandalwood. As recently reported by our group, PT was shown to be effective in the treatment of melanoma. Counterintuitively, PT is not effective (cytotoxic) against melanoma in vitro, and only under in vivo conditions does PT display its anticancer activity. This study elucidated that PT can be effective against melanoma through the inhibition of adrenocorticotropic hormone production in the brain of a mouse, which weakens the Nrf2-dependent antioxidant defenses of melanoma and also pancreatic cancers. This results in both the inhibition of tumor growth and sensitization of the tumor to oxidative stress. Moreover, PT can promote cancer cell death via a mechanism involving lysosomal membrane permeabilization. Different grades of susceptibility were observed among the different cancer cells depending on their lysosomal heat shock protein 70 content, a known stabilizer of lysosomal membranes. In addition, the safety of PT administered i.v. has been evaluated in mice. PT was found to be pharmacologically safe because it showed no organ-specific or systemic toxicity (including tissue histopathologic examination and regular hematology and clinical chemistry data) even when administered i.v. at a high dose (30 mg/kg per day × 23 days). Moreover, new pharmacological advances are being developed to increase its bioavailability and, thereby, its bioefficacy. Therefore, although applications of PT in cancer therapy are just beginning to be explored, it represents a potential (and effective) adjuvant/sensitizing therapy which may improve the results of various oncotherapies. The aim of this review is to present and discuss the results that in our opinion best support the usefulness of PT in cancer therapy, making special emphasis on the in vivo evidence.
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Knez Marevci, Maša, Sašo Bjelić, Barbara Dariš, Željko Knez, and Maja Leitgeb. "The Influence of Extracts from Common Houseleek (Sempervivum tectorum) on the Metabolic Activity of Human Melanoma Cells WM-266-4." Processes 9, no. 9 (August 30, 2021): 1549. http://dx.doi.org/10.3390/pr9091549.
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Human melanoma cells are known as one of the most aggressive cancer cells, and consequently, melanoma is one of the most incurable cancer diseases. There is intense activity in research and development of potential medicines for malignant diseases, including alternative forms of remedies. Therefore, the purpose of our work was testing extracts from the common houseleek (Sempervivum tectorum) grown in Slovenia to establish its impact on human melanoma cells. Namely, we wanted to verify if the extracts inhibit growth of malignant cells and their metabolic activity. Soxhlet, cold solvent, ultrasound, and supercritical extraction methods were applied to obtain S. tectorum extracts. Polyphenols and proanthocyanins content in acquired extracts was determined as well as their antioxidative potential. For a relevant comparison, Chinese (CHI) dried and Slovenian (SLO) lyophilized S. tectorum was used. Results showed that the highest contents of polyphenols and proanthocyanins were yielded from lyophilized material, which also had the highest antioxidative potential. The focus of our work was on analysis of possible inhibition effects of the extracts on human melanoma cells since no past studies were found regarding the possible effects of S. tectorum on metabolic activity of WM-266-4. We established that in a 24-h incubation period, the extracts inhibited metabolic activity of the cells at their concentrations of 20, 10, 4, 2, 1, 0.2, and 0.02 mg/mL. Extract obtained from SLO S. tectorum (ultrasound extraction with acetone as a solvent), which showed promising results of inhibitory effect on the mentioned cells, was further described since the local plant was the focus of our study. CHI S. tectorum extract (Soxhlet extraction with ehtanol:water mixture = 1:1 as a solvent) showed the highest inhibitory effect on human melanoma cells WM-266-4, although both obtained extracts are suitable for their growth inhibition of this specific cell line. Our results suggest inhibitory ability of S. tectorum extracts on the metabolic activity of WM-266-4 metastatic cell line, suggesting their potential use as an anticancer agent.
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Szabo, Katalin, Laura Mitrea, Lavinia Florina Călinoiu, Bernadette-Emőke Teleky, Gheorghe Adrian Martău, Diana Plamada, Mihaela Stefana Pascuta, Silvia-Amalia Nemeş, Rodica-Anita Varvara, and Dan Cristian Vodnar. "Natural Polyphenol Recovery from Apple-, Cereal-, and Tomato-Processing By-Products and Related Health-Promoting Properties." Molecules 27, no. 22 (November 17, 2022): 7977. http://dx.doi.org/10.3390/molecules27227977.
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Polyphenols of plant origin are a broad family of secondary metabolites that range from basic phenolic acids to more complex compounds such as stilbenes, flavonoids, and tannins, all of which have several phenol units in their structure. Considerable health benefits, such as having prebiotic potential and cardio-protective and weight control effects, have been linked to diets based on polyphenol-enriched foods and plant-based products, indicating the potential role of these substances in the prevention or treatment of numerous pathologies. The most representative phenolic compounds in apple pomace are phloridzin, chlorogenic acid, and epicatechin, with major health implications in diabetes, cancer, and cardiovascular and neurocognitive diseases. The cereal byproducts are rich in flavonoids (cyanidin 3-glucoside) and phenolic acids (ferulic acid), all with significant results in reducing the incidence of noncommunicable diseases. Quercetin, naringenin, and rutin are the predominant phenolic molecules in tomato by-products, having important antioxidant and antimicrobial activities. The present understanding of the functionality of polyphenols in health outcomes, specifically, noncommunicable illnesses, is summarized in this review, focusing on the applicability of this evidence in three extensive agrifood industries (apple, cereal, and tomato processing). Moreover, the reintegration of by-products into the food chain via functional food products and personalized nutrition (e.g., 3D food printing) is detailed, supporting a novel direction to be explored within the circular economy concept.
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Spissu, Ylenia, Katarzyna Angelika Gil, Antonio Dore, Giulia Sanna, Giuseppe Palmieri, Andrea Sanna, Maurizio Cossu, et al. "Anti- and Pro-Oxidant Activity of Polyphenols Extracts of Syrah and Chardonnay Grapevine Pomaces on Melanoma Cancer Cells." Antioxidants 12, no. 1 (December 29, 2022): 80. http://dx.doi.org/10.3390/antiox12010080.
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The phenolic composition of Syrah and Chardonnay grape pomaces was studied to assess their antioxidant and prooxidant properties. Polyphenols were extracted by a "green" hydroalcoholic solvent (ethanol/water 1:1 v/v), and a detailed chemical and electrochemical characterization of the phenolic compounds was performed. The antioxidant and prooxidant capacity of the pomace was first studied by cyclic voltammetry (CV) and other reference analytical assays, then with biological tests on B16F10 metastatic melanoma cancer cells. Electrochemical data showed that, when a +0.5 V potential was applied, a low to moderate antioxidant capacity was observed. MTT test showed an increasing viability of melanoma cells, after treatments at low concentration (up to 100 µg/mL) and for a short time (6 h), but when cells were treated with higher doses of extract (≥ 250 µg/mL for 12/24 h), their viability decreased from 25 to 50% vs. control, depending on treatment time, dose, and extract origin. A stronger prooxidant activity resulted when 250 µg/mL of extract was combined with non-toxic doses of H2O2; this activity was correlated with the presence of copper in the extracts. This study shows the potential of winemaking by-products and suggests the opportunity to exploit them for the production of cosmeceuticals, or for combined therapies with approved anticancer drugs.
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Gonzali, Silvia, and Pierdomenico Perata. "Anthocyanins from Purple Tomatoes as Novel Antioxidants to Promote Human Health." Antioxidants 9, no. 10 (October 20, 2020): 1017. http://dx.doi.org/10.3390/antiox9101017.
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Anthocyanins are plant secondary metabolites belonging to the class of polyphenols, whose beneficial roles in the prevention and treatment of several important human diseases have been demonstrated in many epidemiological studies. Their intake through diet strictly depends on the eating habits, as anthocyanins are contained in red and purple fruit and vegetables as well as in some processed foods and beverages, such as red wine. Genetic engineering and breeding programs have been recently carried out to increase the content of anthocyanins in candidate plant species which cannot offer satisfactory levels of these precious compounds. Tomato (Solanum lycopersicum) is a vegetable commodity where these strategies have resulted in success, leading to the production of new anthocyanin-rich fruit varieties, some of which are already marketed. These varieties produce purple fruits with a high nutraceutical value, combining the health benefits of the anthocyanins to the other classical tomato phytochemicals, particularly carotenoids. The antioxidant capacity in tomato purple fruits is higher than in non-anthocyanin tomatoes and their healthy role has already been demonstrated in both in vitro and in vivo studies. Recent evidence has indicated a particular capacity of tomato fruit anthocyanins to act as scavengers of harmful reactive chemical species and inhibitors of proliferating cancer cells, as well as anti-inflammatory molecules.
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Luz, Jefferson Romáryo Duarte da, Jorge A. López, Macelia Pinheiro Ferreira, Rubiamara Mauricio de Sousa, Saulo Victor e. Silva, Maria das Graças Almeida, and Gabriel Araujo-Silva. "In Vitro Antithrombotic, Antitumor and Antiangiogenic Activities of Green Tea Polyphenols and Its Main Constituent Epigallocatechin-3-gallate." Processes 11, no. 1 (December 28, 2022): 76. http://dx.doi.org/10.3390/pr11010076.
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The balance between embolic risk and bleeding represents a clinical challenge in cancer patient treatment, encouraging studies on adjuvant oncologic treatments. Thereby, this study evaluated the in vitro effect of green tea extract (GTE) and epigallocatechin-3-gallate (EGCG) on hemostasis modulation and the antineoplastic effect on melanoma cells (B16-F10) by applying platelet aggregation, angiogenesis and viability cell assays. The results displayed a significant platelet antiaggregant effect, corresponding to 50 and 80% for the extract and EGCG, respectively, compared to the negative control. Furthermore, both GTE and EGCG exhibited antitumor effects by reducing melanoma cell growth by 25 and 50%, respectively, verified by cellular apoptosis. Regarding angiogenesis, these substances inhibited blood vessel formation, reaching about 25% and 99% for GTE and EGCG at 100 μg/mL, respectively. Moreover, TNF-α cell stimulation evidenced VEGF and IL-8 secretion inhibition at 55 and 20% with GTE, while EGCG promoted an inhibition around 78% for both VEGF and IL-8. The results indicate the promising performance of GTE and EGCG as an option for treating cancer and its side effects. Nonetheless, further studies are required to elucidate their action mechanism on clotting, cell death and angiogenesis.
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Prasad, Ram, and Santosh K. Katiyar. "Polyphenols from green tea inhibit the growth of melanoma cells through inhibition of class I histone deacetylases and induction of DNA damage." Genes & Cancer 6, no. 1-2 (February 14, 2015): 49–61. http://dx.doi.org/10.18632/genesandcancer.52.
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Wu, W. B., H. S. Chiang, J. Y. Fang, and C. F. Hung. "Inhibitory effect of lycopene on PDGF-BB-induced signalling and migration in human dermal fibroblasts: a possible target for cancer." Biochemical Society Transactions 35, no. 5 (October 25, 2007): 1377–78. http://dx.doi.org/10.1042/bst0351377.
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Tumours are complex tissues composed of both matrix proteins and stromal cells such as fibroblasts and inflammatory cells. Tumour progression is often the result of dynamic interactions between the tumour cells and their surroundings. Lycopene, a natural carotenoid that is abundant in tomato, has been shown to inhibit proliferation of several types of cancer cells through arrest of tumour cell-cycle progression, IGF-1 (insulin-like growth factor 1) signalling transduction, induction of apoptosis etc. However, in our recent study, we found that lycopene inhibited PDGF-BB (platelet-derived growth factor-BB)-induced signalling and cell migration in human cultured skin fibroblasts through a novel mechanism of action, i.e. direct binding to PDGF-BB. Trapping of PDGF by lycopene also compromised melanoma-induced fibroblast migration and attenuated signalling transduction in fibroblasts simulated by melanoma-derived conditioned medium, suggesting that lycopene may interfere with tumour–stroma interactions. The trapping activity of lycopene on PDGF suggests that it may act as an inhibitor on stromal cells, tumour cells and their interactions, which may contribute to its anti-tumour activity.
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Urbonavičienė, Dalia, Česlovas Bobinas, Ramunė Bobinaitė, Lina Raudonė, Sonata Trumbeckaitė, Jonas Viškelis, and Pranas Viškelis. "Composition and Antioxidant Activity, Supercritical Carbon Dioxide Extraction Extracts, and Residue after Extraction of Biologically Active Compounds from Freeze-Dried Tomato Matrix." Processes 9, no. 3 (March 5, 2021): 467. http://dx.doi.org/10.3390/pr9030467.
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Supercritical carbon dioxide extraction (SCE-CO2) is an attractive, green technology that is used for the recovery of biologically active compounds from plant material. The antioxidant potential of lipophilic fractions (extract obtained with SCE-CO2) and hydrophilic fractions (extracts obtained from the residue after extraction) obtained from a matrix of freeze-dried tomatoes (cvs. “Admiro” F1, “Jurgiai”, “Vilina”, “Pirmutis”, and “Skariai”) was assessed via different antioxidant activity methods. The total amount of polyphenols, carotenoids, and carotenoid isomers before and after SCE-CO2 extraction was also determined. To investigate the effect of the SCE-CO2 extract on the viability of cancer cells, rat glioblastoma C6 cells were chosen. The SCE-CO2 yielded an average of 800 mg of lipophilic fraction per 100 g of freeze-dried tomatoes. The ABTS•+ scavenging activity of the extract was 251 ± 3.4 µmol TE/g. After SCE-CO2 extraction, the DPPH•-RSA of the freeze-dried tomato matrix was 7 to 12% higher. There was a strong positive correlation (R = 0.84) between the total polyphenolics content and the DPPH•-RSA of the tomato samples. The SCE-CO2 increased the radical scavenging activity of the extraction residue, indicating that a considerable fraction of the hydrophilic compounds with particular antioxidant capacity remain unextracted from the tomato matrix. Our results reveal the cytotoxic effect of lycopene extract rich in cis-isomers (62% cis-isomers of the total lycopene content) on rat glioblastoma C6 cells. The viability of the glioblastoma C6 cells significantly decreased (−42%) at a total lycopene concentration of 2.4 µM after 24 h of incubation.
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Vitalini, Sara, Marta Di Martile, Vittoria Cicaloni, Matteo Iannone, Laura Salvini, Donatella Del Bufalo, Marcello Iriti, and Stefania Garzoli. "Volatile and Non-Volatile Content Determination and Biological Activity Evaluation of Fresh Humulus lupulus L. (cv. Chinook) Leaves and Inflorescences." Separations 10, no. 2 (January 29, 2023): 91. http://dx.doi.org/10.3390/separations10020091.
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In this work, the fresh leaves and inflorescences of Humulus lupulus L. cv. Chinook hops were investigated in order to describe their chemical composition and evaluate their biological activities. The analyses were carried out first on fresh untreated samples and then on pulverized ones using the SPME-GC-MS technique. In total, forty-two molecules belonging to different chemical classes were identified, and among these, twenty-three were terpene compounds. In order to carry out the activity assays, the powders were subjected to extraction with two different solvents (methanol and distilled water) by stirring and subsequent sonication at room temperature. To chemically characterize the extracts, the methanolic ones were analyzed by direct injection into the GC-MS apparatus, while the aqueous ones were analyzed by DI-SPME-GC-MS. In addition, with the aim to obtain information on the non-volatile content of the methanolic extracts, they were also subjected to derivatization, and the silylated derivatives were analyzed by GC-MS. The antioxidant activity was then evaluated by means of DPPH and ABTS assays after the determination of the total content of polyphenols and flavonoids. The greatest effects were observed on the methanolic extracts rather than on the aqueous ones. Furthermore, a preliminary study on the cytotoxic power of the methanolic extracts was also conducted on three different human cancer cell lines, such as non-small cell lung cancer (H1299), melanoma (A375) and breast cancer (MCF7). The obtained results showed that the two extracts induced a marginal effect on reducing breast tumor, melanoma and non-small cell lung cancer cell proliferation.
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Jabłońska-Trypuć, Agata, Urszula Wydro, Elżbieta Wołejko, Grzegorz Świderski, and Włodzimierz Lewandowski. "Biological Activity of New Cichoric Acid–Metal Complexes in Bacterial Strains, Yeast-Like Fungi, and Human Cell Cultures In Vitro." Nutrients 12, no. 1 (January 6, 2020): 154. http://dx.doi.org/10.3390/nu12010154.
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Cichoric acid (CA) belongs to the group of polyphenols, which occurs in a variety of plant species and it is characterized by anticancer, antibacterial, and antiviral properties. Selected polyphenols have the ability to combine with metal ions to form chelate complexes that reveal greater biological activity than free compounds. In order to study possible antimicrobial and anticancer effect of CA and its complexes with copper(II)/zinc(II)/nickel(II)/cobalt(II) we decided to conduct cytotoxicity tests to estimate the most effective concentrations of tested compounds. The results of the presented study demonstrated, for the first time, that the treatment with newly synthesized CA-metal complexes has anticancer and antimicrobial effects, which were examined in seven different cell lines: MCF-7, MDA-MB-231, and ZR-75-1 breast cancer cell lines, A375 melanoma cell line, DLD-1 cell line, LN-229 cell line, FN cell line; five bacterial strains: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, Proteus vulgaris, Lactobacillus rhamnosus, yeast Sacchcaromyces boulardii, and pathogenic yeast-like fungi Candida albicans. The presented study indicates that CA-metal complexes could be considered as a potential supplementary tool in anticancer therapy, however, because of their possible toxic activity on fibroblasts, they should be used with caution. Some of the tested complexes have also preservative properties and positive influence on normal non-pathogenic microorganisms, which was demonstrated in selected microbial strains, therefore they may serve as food preservatives of natural origin with cytoprotective properties.
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Wang, Shihua, Valerie L. DeGroff, and Steven K. Clinton. "Tomato and Soy Polyphenols Reduce Insulin-Like Growth Factor-I–Stimulated Rat Prostate Cancer Cell Proliferation and Apoptotic Resistance In Vitro via Inhibition of Intracellular Signaling Pathways Involving Tyrosine Kinase." Journal of Nutrition 133, no. 7 (July 1, 2003): 2367–76. http://dx.doi.org/10.1093/jn/133.7.2367.
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Danciu, Corina, Oana Cioanca, Claudia Watz Farcaș, Monica Hancianu, Roxana Racoviceanu, Delia Muntean, Istvan Zupko, et al. "Botanical Therapeutics (Part II): Antimicrobial and In Vitro Anticancer Activity against MCF7 Human Breast Cancer Cells of Chamomile, Parsley and Celery Alcoholic Extracts." Anti-Cancer Agents in Medicinal Chemistry 21, no. 2 (December 31, 2020): 187–200. http://dx.doi.org/10.2174/1871520620666200807213734.
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Background: This study was designed as a continuation of a complex investigation about the phytochemical composition and biological activity of chamomile, parsley, and celery extracts against A375 human melanoma and dendritic cells. Objective: The main aim was the evaluation of the antimicrobial potential of selected extracts as well as the in vitro anticancer activity against MCF7 human breast cancer cells. Methods: In order to complete the picture regarding the phytochemical composition, molecular fingerprint was sketched out by the help of FTIR spectroscopy. The activity of two enzymes (acetylcholinesterase and butyrylcholinesterase) after incubation with the three extracts was spectrophotometrically assessed. The antimicrobial potential was evaluated by disk diffusion method. The in vitro anticancer potential against MCF7 human breast cancer cells was appraised by MTT, LDH, wound healing, cell cycle, DAPI, Annexin-V-PI assays. Results: The results showed variations between the investigated extracts in terms of inhibitory activity against enzymes, such as acetyl- and butyrilcholinesterase. Chamomile and parsley extracts were active only against tested Gram-positive cocci, while all tested extracts displayed antifungal effects. Among the screened samples at the highest tested concentration, namely 60μg/mL, parsley was the most active extract in terms of reducing the viability of MCF7 - human breast adenocarcinoma cell line and inducing the release of lactate dehydrogenase. On the other hand, chamomile and celery extracts manifested potent anti-migratory effects. Furthermore, celery extract was the most active in terms of total apoptotic events, while chamomile extract induced the highest necrosis rate. Conclusion: The screened samples containing phytochemicals belonging in majority to the class of flavonoids and polyphenols can represent candidates for antimicrobial and anticancer agents.
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Georgieva, Almira, Yana Ilieva, Zlatina Kokanova-Nedialkova, Maya Margaritova Zaharieva, Paraskev Nedialkov, Ana Dobreva, Alexander Kroumov, Hristo Najdenski, and Milka Mileva. "Redox-Modulating Capacity and Antineoplastic Activity of Wastewater Obtained from the Distillation of the Essential Oils of Four Bulgarian Oil-Bearing Roses." Antioxidants 10, no. 10 (October 14, 2021): 1615. http://dx.doi.org/10.3390/antiox10101615.
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The wastewater from the distillation of rose oils is discharged directly into the soil because it has a limited potential for future applications. The aim of the present study was to determine in vitro the chromatographic profile, redox-modulating capacity, and antineoplastic activity of wastewater obtained by distillation of essential oils from the Bulgarian Rosa alba L., Rosa damascena Mill., Rosa gallica L., and Rosa centifolia L. We applied UHPLC-HRMS for chromatographic analysis of rose wastewaters, studied their metal-chelating and Fe(III)-reducing ability, and performed MTT assay for the evaluation of cytotoxic potential against three tumorigenic (HEPG2—hepatocellular adenocarcinoma, A-375—malignant melanoma, A-431—non-melanoma epidermoid squamous skin carcinoma) and one non-tumorigenic human cell lines (HaCaT—immortalized keratinocytes). The median inhibitory concentrations (IC50) were calculated with nonlinear modeling using the MAPLE® platform. The potential of the wastewaters to induce apoptosis was also examined. Mono-, di-, and acylated glycosides of quercetin and kaempferol, ellagic acid and its derivatives as main chemical components, and gallic acid and its derivatives—such as catechin and epicatechin—were identified. The redox-modulating capacity of the samples (TPTZ test) showed that all four wastewaters exhibited the properties of excellent heavy metal cleaners, but did not exert very strong cytotoxic effects. The lowest IC50 rate was provided in wastewater from R. centifolia (34–35 µg/mL of gallic acid equivalents after a 72 h period for all cell lines). At 24 and 48 hours, the most resistant cell line was HEPG2, followed by HaCaT. After 72 h of exposure, the IC50 values were similar for tumor and normal cells. Still, R. damascena had a selectivity index over 2.0 regarding A-431 non-melanoma skin cancer cells, showing a good toxicological safety profile in addition to moderate activity—IC50 of 35 µg/mL polyphenols. The obtained results related to wastewaters acquired after the distillation of essential oils from the Bulgarian R. alba, R. damascena, R. gallica, and R. centifolia direct our attention to further studies for in-depth elucidation of their application as detoxifying agents under oxidative damage conditions in other experimental datasets.
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Aguiar, Joselin, João L. Gonçalves, Vera L. Alves, and José S. Câmara. "Relationship between Volatile Composition and Bioactive Potential of Vegetables and Fruits of Regular Consumption—An Integrative Approach." Molecules 26, no. 12 (June 15, 2021): 3653. http://dx.doi.org/10.3390/molecules26123653.
Full textAbstract:
In recent years, there has been a growing interest in studying and exploring the potential health benefits of foods, mainly from vegetables and fruits from regular intake. The presence of secondary metabolites, namely polyphenols, carotenoids and terpenes, in certain food matrices seems to contribute to their functional properties, expressed through an increased prevention in the development of certain chronic diseases, namely coronary heart diseases, neurodegenerative diseases, cancer and diabetes. However, some foods’ volatile secondary metabolites also present important bioactive properties, although this is a poorly scientifically explored field. In this context, and in order to explore the potential bioactivity of volatile metabolites in different vegetables and fruits from regular consumption, the volatile composition was established using a green extraction technique, solid phase microextraction in headspace mode (HS-SPME), combined with gas chromatography tandem mass spectrometry (GC-MS). A total of 320 volatile metabolites, comprising 51 terpenic compounds, 45 organosulfur compounds, 31 aldehydes, 37 esters, 29 ketones, 28 alcohols, 23 furanic compounds, 22 hydrocarbons, 19 benzene compounds, 13 nitrogenous compounds, 9 carboxylic acids, 7 ethers, 4 halogenated compounds and 3 naphthalene derivatives, were positively identified. Each investigated fruit and vegetable showed a specific volatile metabolomic profile. The obtained results revealed that terpenic compounds, to which are associated antimicrobial, antioxidant, and anticancer activities, are the most predominant chemical family in beetroot (61%), orange carrot (58%) and white carrot (61%), while organosulfur compounds (antiviral activity) are dominant in onion, garlic and watercress. Broccoli and spinach are essentially constituted by alcohols and aldehydes (enzyme-inhibition and antimicrobial properties), while fruits from the Solanaceae family are characterized by esters in tamarillo and aldehydes in tomato.
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Buda, Valentina, Ana-Maria Brezoiu, Daniela Berger, Ioana Zinuca Pavel, Delia Muntean, Daliana Minda, Cristina Adriana Dehelean, et al. "Biological Evaluation of Black Chokeberry Extract Free and Embedded in Two Mesoporous Silica-Type Matrices." Pharmaceutics 12, no. 9 (September 1, 2020): 838. http://dx.doi.org/10.3390/pharmaceutics12090838.
Full textAbstract:
Black chokeberry fruits possess a wide range of biological activities, among which the most important that are frequently mentioned in the literature are their antioxidant, anti-inflammatory, anti-proliferative, and antimicrobial properties. The present paper reports, for the first time, the encapsulation of the ethanolic extract of Aronia melanocarpa L. fruits into two mesoporous silica-type matrices (i.e., pristine MCM-41 and MCM-41 silica decorated with zinc oxide nanoparticles). The aim of this work was to evaluate the antiradicalic capacity, the antimicrobial potential, and the effects on the cell viability on a cancer cell line (i.e., A375 human melanoma cell line) versus normal cells (i.e., HaCaT human keratinocytes) of black chokeberry extract loaded on silica-type matrices in comparison to that of the extract alone. The ethanolic polyphenolic extract obtained by conventional extraction was characterized by high-performance liquid chromatography with a photodiode array detector (HPLC–PDA) and spectrophotometric methods. The extract was found to contain high amounts of polyphenols and flavonoids, as well as good radical scavenging activity. The extract-loaded materials were investigated by Fourier transform infrared spectroscopy, N2 adsorption–desorption isotherms, thermal analysis, and radical scavenger activity on solid samples. The black chokeberry extract, both free and loaded onto mesoporous silica-type matrices, exhibited a significant antioxidant capacity. Antibacterial activity was recorded only for Gram-positive bacteria, with a more potent antibacterial effect being observed for the extract loaded onto the ZnO-modified MCM-41 silica-type support than for the free extract, probably due to the synergistic effect of the ZnO nanoparticles that decorate the pore walls of silica. The cellular viability test (i.e., MTT assay) showed dose- and time-dependent activity regarding the melanoma cell line. The healthy cells were less affected than the cancer cells, with all tested samples showing good cytocompatibility at doses of up to 100 µg/mL. Improved in vitro antiproliferative and antimigratory (i.e., scratch assay) potential was demonstrated through the loading of black chokeberry extract into mesoporous silica-type matrices, and the screened samples exhibited low selectivity against the tested non-tumor cell line. Based on presented results, one can conclude that mesoporous silica-type matrices are good hosts for black chokeberry extract, increasing its antioxidant, antibacterial (on the screened strains), and in vitro antitumor (on the screened cell line) properties.
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Sufianova, Galina, Ilgiz Gareev, Ozal Beylerli, Jianing Wu, Alina Shumadalova, Albert Sufianov, Xin Chen, and Shiguang Zhao. "Modern aspects of the use of natural polyphenols in tumor prevention and therapy." Frontiers in Cell and Developmental Biology 10 (September 12, 2022). http://dx.doi.org/10.3389/fcell.2022.1011435.
Full textAbstract:
Polyphenols are secondary plant metabolites or organic compounds synthesized by them. In other words, these are molecules that are found in plants. Due to the wide variety of polyphenols and the plants in which they are found, these compounds are divided according to the source of origin, the function of the polyphenols, and their chemical structure; where the main ones are flavonoids. All the beneficial properties of polyphenols have not yet been studied, since this group of substances is very extensive and diverse. However, most polyphenols are known to be powerful antioxidants and have anti-inflammatory effects. Polyphenols help fight cell damage caused by free radicals and immune system components. In particular, polyphenols are credited with a preventive effect that helps protect the body from certain forms of cancer. The onset and progression of tumors may be related directly to oxidative stress, or inflammation. These processes can increase the amount of DNA damage and lead to loss of control over cell division. A number of studies have shown that oxidative stress uncontrolled by antioxidants or an uncontrolled and prolonged inflammatory process increases the risk of developing sarcoma, melanoma, and breast, lung, liver, and prostate cancer. Therefore, a more in-depth study of the effect of polyphenolic compounds on certain signaling pathways that determine the complex cascade of oncogenesis is a promising direction in the search for new methods for the prevention and treatment of tumors.
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Prieto, Karol, Yu Cao, Eslam Mohamed, Jimena Trillo-Tinoco, Rosa A. Sierra, Claudia Urueña, Tito Alejandro Sandoval, Susana Fiorentino, Paulo C. Rodriguez, and Alfonso Barreto. "Polyphenol-rich extract induces apoptosis with immunogenic markers in melanoma cells through the ER stress-associated kinase PERK." Cell Death Discovery 5, no. 1 (September 9, 2019). http://dx.doi.org/10.1038/s41420-019-0214-2.
Full textAbstract:
Abstract Polyphenols elicit antitumor activities, in part, through the induction of anti- or pro-oxidant effects in cancer cells which promote priming of protective anti-tumor immunity. We recently characterized a polyphenol-rich extract from Caesalpinia spinosa (P2Et) that stimulates in vivo antitumor responses against breast and melanoma tumor models via the promotion of immunogenic cancer cell death (ICD). However, the primary mediators whereby P2Et promotes ICD remained unknown. Here, we sought to elucidate the role that severe endoplasmic reticulum (ER) stress plays in mediating P2Et-induced apoptosis and ICD in murine melanoma cells. Our findings demonstrate a substantial selective induction of specific ER-stress mediators in B16-F10 melanoma cells treated with P2Et. While knockout of the ER stress-associated PKR-like ER kinase (PERK) prevented induction of apoptosis and expression of ICD markers in P2Et-treated cells, deletion of X-box binding protein 1 (Xbp1) did not. P2Et-driven activation of PERK in melanoma cells was found to promote ER-calcium release, disrupt mitochondrial membrane potential, and trigger upregulation of ICD drivers, surface calreticulin expression, and extracellular release of ATP and HMGB1. Notably, calcium release inhibition, but not targeting of PERK-driven integrated stress responses, prevented P2Et-induced apoptosis. Collectively, these results underline the central role of PERK-directed calcium release in mediating the antitumor and immunogenic actions of P2Et in melanoma cells.
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Das, Rina, Dinesh Kumar Mehta, and Meenakshi Dhanawat. "Medicinal Plants in Cancer Treatment: Contribution of Nuclear Factor-Kappa B (NF-kB) Inhibitors." Mini-Reviews in Medicinal Chemistry 22 (March 7, 2022). http://dx.doi.org/10.2174/1389557522666220307170126.
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Abstract: Nuclear factor-kappa B (NF-κB) is one of the principal inducible proteins that is a predominant transcription factor known to control the gene expression in mammals and plays a pivotal role in regulating cell signalling in the body under certain physiological and pathological conditions. In cancer cells, such as colon, breast, pancreatic, ovarian, melanoma, and lymphoma, the NF-κB pathway has been reported to be active. In cellular proliferation, promoting angiogenesis, invasion, metastasis of tumour cells and blocking apoptosis, the constitutive activity of NF-κB signalling has been reported. Therefore, immense attention has been given to developing drugs targeting NF-κB signalling pathways to treat many types of tumours. They are a desirable therapeutic target for drugs, and many studies concentrated on recognizing compounds. They may be able to reverse or standstill the growth and spread of tumours that selectively interfere with this pathway. Recently, numerous substances derived from plants have been evaluated as possible inhibitors of the NF-κB pathway. These include various compounds, such as flavonoids, lignans, diterpenes, sesquiterpenes, polyphenols, etc. A study supported by folk medicine demonstrated that plant-derived compounds could suppress NF-κB signalling. Taking this into account, the present review revealed the anticancer potential of naturally occurring compounds which have been verified both by inhibiting the NF-κB signalling and suppressing growth and spread of cancer and highlighting their mechanism of NF-κB inhibition.
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Perużyńska, Magdalena, Anna Nowak, Radosław Birger, Paula Ossowicz-Rupniewska, Maciej Konopacki, Rafał Rakoczy, Łukasz Kucharski, et al. "Anticancer properties of bacterial cellulose membrane containing ethanolic extract of Epilobium angustifolium L." Frontiers in Bioengineering and Biotechnology 11 (February 20, 2023). http://dx.doi.org/10.3389/fbioe.2023.1133345.
Full textAbstract:
Epilobium angustifolium L. is a medicinal plant well known for its anti-inflammatory, antibacterial, antioxidant, and anticancer properties related to its high polyphenols content. In the present study, we evaluated the antiproliferative properties of ethanolic extract of E. angustifolium (EAE) against normal human fibroblasts (HDF) and selected cancer cell lines, including melanoma (A375), breast (MCF7), colon (HT-29), lung (A549) and liver (HepG2). Next, bacterial cellulose (BC) membranes were applied as a matrix for the controlled delivery of the plant extract (BC-EAE) and characterized by thermogravimetry (TG), infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) images. In addition, EAE loading and kinetic release were defined. Finally, the anticancer activity of BC-EAE was evaluated against the HT-29 cell line, which presented the highest sensitivity to the tested plant extract (IC50 = 61.73 ± 6.42 µM). Our study confirmed the biocompatibility of empty BC and the dose and time-dependent cytotoxicity of the released EAE. The plant extract released from BC-2.5%EAE significantly reduced cell viability to 18.16% and 6.15% of the control values and increased number apoptotic/dead cells up to 37.53% and 66.90% after 48 and 72 h of treatment, respectively. In conclusion, our study has shown that BC membranes could be used as a carrier for the delivery of higher doses of anticancer compounds released in a sustained manner in the target tissue.
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Ferrari, Virginia, Gustavo Rodríguez, Matías González, Esteban Vicente, Gustavo Giménez, Danilo Cabrera, and Facundo Ibañez. "Nutritional Quality and Nutraceutical Potential of Fruits and Vegetables as a tool for Genetic Breeding Programs." Agrociencia Uruguay 25, NE2 (January 5, 2022). http://dx.doi.org/10.31285/agro.25.814.
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Prevention of the development of chronic diseases related to metabolic syndrome has been found to depend on a healthy diet. Among healthy foods, fruits and vegetables play a very important role due to their nutrient content, especially antioxidants, vitamins and polyphenols. For 20 years, INIA has promoted the concept of quality associated to the differentiation of products in the fruit and horticultural sectors, considering aspects not only linked to the organoleptic qualities, but also the production processes that ensure food safety and nutritional contribution. More recently, research works have been implemented to contribute to highlighting the role of fruits and vegetables as nutraceutical foods, contributing to the prevention of chronic non-transmissible human diseases such as diabetes, cancer, or obesity. Also, to contribute to the knowledge of the effects of factors as breeding and culture management on the nutritional potential and promote production, consumption and commercialization. The bioactive compounds content and in vitro antioxidant activity (DPPH and ORAC) were characterized in different genotypes of native fruits, onion, potato, strawberry, sweet potato and tomato. High levels of flavonoids (quercetin) were found in onions and advanced lines. Samples of tomatoes, potatoes, sweet potatoes, and strawberries showed high contents of phenolic compounds, ascorbic acid, anthocyanins, total carotenoids, and total antioxidant capacity (ORAC) with a great interspecies variability; so, they can be included in the development of varieties differentiated by nutraceutical attributes. The analyses conducted also demonstrate the potential of native fruits as sources of essential nutrients, with contents comparable to and even superior to other fruits considered "superfruits". Through selection and breeding, national varieties can be developed with outstanding organoleptic characteristics, good levels of bioactive compounds, and antioxidant properties that contribute to the health of the population.
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Cicero, Arrigo F. G., Olta Allkanjari, Gian Maria Busetto, Tommaso Cai, Gaetano Larganà, Vittorio Magri, Gianpaolo Perletti, et al. "Nutraceutical treatment and prevention of benign prostatic hyperplasia and prostate cancer." Archivio Italiano di Urologia e Andrologia 91, no. 3 (October 2, 2019). http://dx.doi.org/10.4081/aiua.2019.3.139.
Full textAbstract:
During the last years, pharmaceutical innovations in primary care are dramatically less frequent and will be even more rare in the next future. In this context, preclinical and clinical research oriented their interest toward natural compounds efficacy and safety, supporting the development of a new “nutraceutical” science. Medicinal plants, in the form of plant parts or extracts of them, are commonly used for the treatment of prostate diseases such as benign hypertrophy, prostatitis and chronic pelvic pain syndrome. The pharmacological properties searched for the treatment of prostatic diseases are anti-androgenic, anti-estrogenic, antiproliferative, antioxidant and anti-inflammatory. The most studied and used medicinal plants are Serenoa repens, Pygeum africanum and Urtica dioica. Other promising plants are Cucurbita pepo, Epilobium spp, Lycopersum esculentum, Secale cereale, Roystonea regia, Vaccinium macrocarpon. In parallel, epidemiological studies demonstrated that diet may play an important role on incidence and development of prostatic diseases. The Mediterranean diet is rich of elements with anti-oxidant properties that act as a protective factor for prostatic cancer. Similarly, low intake of animal protein, high intake of fruits and vegetable, lycopene and zinc are a protective factor for benign prostatic hyperplasia (BPH). Serenoa repens in the treatment of symptoms of BPH has been tested either alone or, more frequently, in combination with other medicinal plants, alpha-blockers and inhibitors of 5- alpha reductase (5-ARI). Recent meta-analyses found the effectiveness of Serenoa repens similar or inferior of that of finasteride and tamsulosin but clearly higher than that of placebo in the treatment of mild and moderate low urinary tract symptoms (LUTS), nocturia and discomfort. Clinical trials showed potential synergistic effect of Serenoa repens with other medicinal plants and drugs. In addition to Serenoa repens, there are many other medicinal plants for which clinical evidence is still controversial. Urtica dioica, Pygeum africanum and Curcubita pepo can be considered as an adjunct to the common therapies and their use is supported by studies showing improvement of symptoms and flowmetric indices. Lycopene and selenium are natural products with antioxidant and anti-inflammatory action. The combination of lycopene and selenium with Serenoa repens was able to reduce inflammation in histological prostate sections and to further improve symptom scores and urinary flow in patients with BPH on tamsulosin treatment. Similar effects could be obtained with the use of other carotenoids, such as astaxanthin, and/or zinc. Efficacy on symptoms of patients with BPH of some polyphenols such as quercitin, equol and curcumin have been demonstrated by clinical studies. Pollen extract is a mixture of natural components able to inhibit several cytokines and prostaglandin and leukotriene synthesis resulting in a potent anti-inflammatory effect. Pollen extracts significantly improve symptoms, pain, and quality of life in patients affected by chronic pelvic pain syndrome and chronic prostatitis. Beta-sitosterol is a sterol able to improve urinary symptoms and flow measures, but not to reduce the size of the prostate gland. Palmitoylethanolamide (PEA) is an endogenous fatty acid amide-signaling molecule with anti-inflammatory and neuroprotective effects that can have an interesting role in the management of chronic pelvic pain syndrome and chronic urological pain. Finally, several plant-based products have been subjected to preclinical, in vitro and in vivo, investigations for their potential pharmacological activity against prostate cancer. Some epidemiological studies or clinical trials evaluated the effects of beverages, extracts or food preparations on the risk of prostate cancer. Some plant species deserved more intense investigation, such as Camelia sinensis (green or black tea), Solanum lycopersicum (common tomato), Punica granatum (pomegranate), Glycine max (common soy) and Linum usitatissimum (linen).
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WI, Abdel-Fattah, and El-Bassyouni GT. "Medicinal Functions of Physalis Fruits for Biomedical Applications." Austin Journal of Pharmacology and Therapeutics 9, no. 2 (May 24, 2021). http://dx.doi.org/10.26420/austinjpharmacolther.2021.1135.
Full textAbstract:
Drug innovation using natural products is an interesting mission for planning new leads. It describes the bioactive compounds resulting from natural resources, characterization and pharmacological examination. It emphases on the triumph of these resources in the process of finding and realizing new and effective drug compounds that can be beneficial for human resources. For medicinal devotions and for the progress of pharmaceutical substances, medicinal plants were used such as Physalis angulata L which is a medicinal plant used for numerous therapies including wound healing (Figure 1) [1]. Figure 1: Physalis angulata L Fruits within its cover [16]. Physalis peruviana (golden berry) is an herbaceous annual plant belongs to the family Solanaceae [2]. This plant has a tremendous medicinal value for curing out different diseases: cancer, leukemia, diabetes, ulcers, malaria, asthma, hepatitis, dermatitis, rheumatism and several other diseases [3]. The golden berry fruit tastes like a sweet tomato and includes high levels of vitamin C, vitamin A and the vitamin B-complex. The fruit was demonstrated to have both anti-inflammatory and antioxidant properties [4,5]. Herbal specialists and local people of several countries have used many extracts of medicinal plants to achieve and treat various diseases comprising wound healing [6]. Physalis with its notable benefits related to high nutrients and bioactive compounds with extraordinary antioxidant activity and other several medicinal properties have been ascribed to these compounds [7,8]. The bioactive compounds are formed as primary and secondary metabolites of the fruits. These compounds are biologically active with cytotoxic, antimicrobial, antioxidant, antiviral, fungicidal, insecticidal, tranquilizing, analgesic, anti-inflammatory, and contraceptive actions, among others. Such compounds are used for several dedications, such as in medical therapy, to cure diseases, in the cosmetics, and in the food industry as antioxidants or flavorings [9]. Wounds are well-defined as a break in the cellular integrity of the anatomic continuousness of a tissue generally because of a chemical, microbial, physical or thermal injury [10]. Recently, wounds have become a very exciting pathological problem. Abdul-Nasir-Deen et al., explored the anti- inflammatory and wound healing properties of methanol leaf extract of Physalis angulata L [11]. They indicated that such extract possesses anti-inflammatory and wound healing activity which may justify its medicinal uses in the treatment of wounds. The PAL formulated cream at several concentrations of 1.25, 2.5, 5 and 10% w/v verified wound healing properties with obvious angiogenesis, collagenation and re-epithelization distinctive of fibrous tissue formation in wound bed [12] as presented in Figure 2. Figure 2: Therapeutic effect of methanol leaf extract of Physalis angulata on carrageenan- induced oedema in rats. A) Time-course curve; B) Area under the curve of carrageenan induced oedema, Saline: Normal Saline Control group, Aspirin: Aspirin-treated group, PAL: methanol leaf extract of Physalis angulate. Values are mean ± SEM (n=5). Nsp >0.05; *p< 0.05; **p< 0.01; ***p<0.001; yyp< 0.001; yyyp< 0.0001. Compared with normal saline control [11]. Moreover, the methanol leaf extracts of Physalis angulate with the existence of secondary metabolites including flavonoids and tannins justify the biological and pharmacological achieved activities (Figure 3). Figure 3: Histological images (x 400) showing influence of PAL on excised wound tissues from both treated and untreated wound tissues. A) Untreated wound tissues; B) Vehicle treated (aqueous cream only) wound tissues; C) 1% w/w silver sulphadiazine- treated wound tissues; D) 10% w/w PAL-treated wound tissues; E) 5% w/w PAL- treated wound tissues; F) 2.5% w/w PALtreated wound; G) 1.25% w/w PAL-treated wound; DNGT: Diffuse Necrotic Granulation Tissue; MDGT: Moderate Diffuse Granulation Tissue; HF: Hair Follicle; DF: Dense Fibrous Tissue; ASCKE: Atrophic Squamous Cell Keratinized Epithelium; SGT: Reduced Granulation Tissue; SeG: Sebaceous Gland; SwG: Sweat Gland [11]. To formulate a porous carbon material that had an abundance of surface functional groups and a huge specific surface area; Physalis alkekengi L. husk (PH) was used for the first time as a carbon source from PH and designated as porous carbon Physalis alkekengi L. husk (PCPH) by Zhang et al., [13]. The experimental results demonstrate that (PCPH) prepared from PH has good adsorption performance for Malachite Green (MG). MG was used as a model dye for evaluating the adsorption performance of PCPH. Zhang et al concluded that PCPH has excellent application potential in the treatment of environmental water pollution. Therefore, the preparation of PCPH with high adsorption performance has upright scenarios treatment of wastewater from printing and dye industries, and similarly affords a hypothetical basis for the inclusive use of shell-based agricultural waste [14]. The possible adsorption mechanisms of PCPH for MG are anticipated; which comprises H-bond interaction, pore filling, p-p interaction, and electrostatic attraction (Figure 4) [15]. Figure 4: The adsorption mechanism diagram of PCPH to MG [13]. Recently, Zimmer et al., concluded that the extracts of the Physalis fruit have functional properties of great importance, being a source of phenolic compounds possessing antioxidant, antibacterial, and antitumor activities [8]. The pulp and seed extracts displayed moderately active inhibition halos in the existence of Gram-positive bacteria. Both pulp and seeds extracts were talented to reduce the cell viability percentage. The pulp (P) and seed (S) hydroalcoholic extracts of Physalis pubescens showed moderate antibacterial activity against Gram-positive Staphylococcus aureus and Listeria monocytogenes. The pulp (P) and seed (S) extracts showed moderate anti-tumor activity against the rat glioblastoma cell line (C6) and murine melanoma cell line (B16F10) (Figure 5). Figure 5: Viability graphs of cells of murine melanoma lineage (B16F10) exposed to different concentration s for 48h and 72h of hydroalcoholic extracts of pulp (P) and seed (S). *The greater the number of asterisks, the higher the significance [8].