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1

EARDLEY, CONNAL. "A taxonomic revision of the southern African species of dauber bees in the genus Megachile Latreille (Apoidea: Megachilidae)." Zootaxa 3460, no. 1 (September 7, 2012): 1. http://dx.doi.org/10.11646/zootaxa.3460.1.1.

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The five dauber subgenera of Megachile that occur in southern Africa are revised. They are: Gronoceras, Maximegachile,Callomegachile, Chalicodoma and Pseudomegachile. This group comprises 43 valid species, nine of which are new to sci-ence. They are: Megachile (Callomegachile) soutpansbergensis sp.n., Megachile (Chalicodoma) gessorum sp.n., Mega-chile (Chalicodoma) sarahae sp.n., Megachile (Chalicodoma) richtersveldensis sp.n., Megachile (Pseudomegachile) gessisp.n., Megachile (Pseudomegachile) namibensis sp.n., Megachile (Pseudomegachile) pseudotaraxis sp.n., Megachile(Pseudomegachile) taraxis sp.n. and Megachile (Pseudomegachile) pseudotaraxis sp.n. Thirty-four new synonynyms havebeen recorded. Megachile simpsoni race yapiensis Cockerell is a junior synonym of Megachile bombiformis Gerstaecker;Megachile combusta Smith, Megachile nigrocincta Ritsema and Megachile tricolor Friese are synonyms of Megachile cincta(Fabricius); Megachile cerberus var. optima Cockerell is a synonym of Megachile felina Gerstaecker; Megachile chrysor-rhoea Gerstaecker is a synonym of Megachile rufipennis (Fabricius); Megachile perniciosa Friese, Megachile perniciosa var.pallipennis Friese and Megachile aridissima Cockerell are synonyms of Megachile rufiventris Guérin–Méneville; Megachileexcavata Cockerell is a synonym of Megachile demeter (Cockerell); Megachile kamerunensis totafusca Pasteels is a syn-onym of Megachile kamerunensis Friese; Megachile tritacantha Pasteels is a synonym of Megachile sheppardi (Pasteels);Megachile musculus Friese, Megachile johannis Pasteels, Megachile biexcisa Pasteels and Megachile johannis fulvosetosaPasteels are synonyms of Megachile karooensis Brauns; Megachile insolita Pasteels, Megachile reicherti Brauns, Megachileacanthura Cockerell, Megachile bipunctulata Pasteels and Megachile aurulenta Pasteels are synonyms of Megachile muri-na Friese; Megachile cinctiventris Friese, Megachile albopilosa Friese and Megachile lineofasciata Pasteels are synonymsof Megachile niveofasciata Friese; Megachile congruens Friese and Megachile flaviventris Friese are synonyms of Mega-chile schulthessi Friese; Megachile empeyi Pasteels is a synonym of Megachile cradockensis Friese; Megachile torridusSmith, Megachile decemsignata Radoszkowski and Megachile junodi Friese are synonyms of Megachile fervida (Smith);Megachile bullata Friese and Megachile trisecta Pasteels are synonyms of Megachile nasicornis Friese; Megachile laminataFriese and Megachile armatipes Friese are synonyms of Megachile mossambica Gribodo. The nomenclatorial history ofeach species is documented, descriptions are given, food plants are recorded and distribution maps are provided. A key to the included subgenera and keys to the species in each subgenus are given.
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2

Engel, Michael S. "Replacement names for bees in the tribe Megachilini (Hymenoptera: Megachilidae)." Journal of Melittology, no. 70 (June 29, 2017): 1–5. http://dx.doi.org/10.17161/jom.v0i70.6558.

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Eight instances of homonymy are identified in the megachiline genera Coelioxys Latreille and Megachile Latreille, with replacement names established for the junior homonyms. Coelioxys (Coelioxys) lethosyne Engel, new name, is proposed for C. simplex latefasciata Friese (nec Morawitz); C. (Liothyrapis) wuae Engel, new name, is proposed for C. albofasciata Wu (nec Radoszkowski); Megachile (Eutricharaea) hisarica Engel, new name, is proposed for M. dentata Rahman & Chopra (nec Friese); M. (Megachile) agnosta Engel, new name, is proposed for M. japonica Matsumura (nec Alfken); M. (Dasymegachile) schwimmeri Engel, new name, is proposed for M. mitchelli Raw (nec Gupta); M. (Creightonella) taftanica Engel, new name, is proposed for M. aurantiaca Rebmann (nec Friese); M. (Callomegachile) biseta austrina Engel, new name, is proposed for M. biseta australis Pasteels (nec Lucas); and M. (C.) ceratops Engel, new name, is proposed for M. monoceros Friese (nec Pallas).
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3

Kumari, Priyanka, and Neelima R. Kumar. "A new species from Genus Megachile Subgenus Callomegachile (Hymenoptera, Megachilidae) from Chandigarh and Punjab plains." Journal of Applied and Natural Science 10, no. 1 (March 1, 2018): 454–58. http://dx.doi.org/10.31018/jans.v10i1.1650.

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A new species Megachile pseudodisjuncta of the bee is being reported from Genus Megachile and Subgenus Callomegachile. It bears a close resemblance to M. disjuncta from which it has been distinguished on the basis of certain morphological characters and genitalia. Megachilid bees bear utmost significance because they are very good pollinators of both cultivated and wild fauna. Consequently, their taxonomic identification and conservation are very crucial. The detailed taxonomically important morphological characters of both the species were studied and photographed. Male sternum and genitalia were studied microscopically and photographed. The material examined and floral associations have also been presented in the paper.
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4

EARDLEY, CONNAL. "A taxonomic revision of the southern African leaf-cutter bees, Megachile Latreille sensu stricto and Heriadopsis Cockerell (Hymenoptera: Apoidea: Megachilidae)." Zootaxa 3601, no. 1 (January 11, 2013): 1–134. http://dx.doi.org/10.11646/zootaxa.3601.1.1.

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The five southern African subgenera of Megachile with recessed cutting edges between their teeth are revised. The entire group comprises 37 valid species, two of which are new: Megachile (Eutricharaea) gobabebensis sp. n. and Megachile (Eutricharaea) goegabensis sp. n. Other species are: Megachile (Amegachile) fimbriata Smith, Megachile (Amegachile) nasalis Smith, Megachile (Amegachile) bituberculata Ritsema, Megachile (Eutricharaea) afra Pasteels, Megachile (Eutricharaea) aurifera Cockerell, Megachile (Eutricharaea) barbata Smith, Megachile (Eutricharaea) basalis Smith, Megachile (Eutricharaea) bucephala (Fabricius), Megachile (Eutricharaea) cyanescens Friese, Megachile (Eutricharaea) eurymera Smith, Megachile (Eutricharaea) familiaris Cockerell, Megachile (Eutricharaea) konowiana Friese, Megachile (Eutricharaea) meadewaldoi Brauns, Megachile (Eutricharaea) muansae Friese, Megachile (Eutricharaea) pachyceps Friese, Megachile (Eutricharaea) regina Friese, Megachile (Eutricharaea) salsburyana Friese, Megachile (Eutricharaea) venusta Smith, Megachile (Eutricharaea) wahlbergi Friese, Megachile (Heriadopsis) whiteana Cameron, Megachile (Paracella) admixta Cockerell, Megachile (Paracella) barkeri Cockerell, Megachile (Paracella) chrysopogon Vachal, Megachile (Paracella) curtula Gerstaecker, Megachile (Paracella) edwardsi Friese, Megachile (Paracella) filicornis Friese, Megachile(Paracella) frontalis Smith, Megachile (Paracella) malangensis Friese, Megachile (Paracella) pilosella Friese, Megachile (Paracella) semierma Vachal, Megachile (Paracella) ungulata Smith, Megachile (Platysta) khamana Cockerell. Of the 78 new synonymies fimbriata vulpecula Pasteels is M. fimbriata; volkmanni ventrifasciata Strand is M. nasalis; sjoestedti var. rubripedana Strand is M. tuberculata; gratiosa Gerstaecker,concinna Smith, marusa Cameron, robertiana Cameron, venustella Cockerell, umbiloensis Cockerell and acallognatha Cockerell are M. venusta Smith; latimetatarsis Strand and rozenii Pasteels are M. basalis Smith; semifulva Friese and planatipes Cockerell are M. bucephala (Fabricius); seclusiformis Cockerell is M. salsburyana Friese; flava Friese and rhodoleucura Cockerell are M. eurymera Smith; luteola Pasteels and stellensis Pasteels are M. familiaris Cockerell; nasutula Brauns, coelostoma Cockerell, and nitidicauda Cockerell are M. barbata Smith; venustoides Strand, venustella zambesica Cockerell and pondonis Cockerell are wahlbergi Friese; okanjandica Strand and vittatula Cockerell are cyanescens Friese; leucospilura Cockerell is M. muansae Friese; cordata Smith,tardula Cameron, ekuivella Cockerell, krebsiana Strand, rhodesica Cockerell, natalica Cockerell, masaiella Cockerell, chromatica Cockerell, gratiosella Cockerell, rhodesica haematognatha Cockerell, mackieae Cockerell,flammicauda Cockerell, venusta var. semiflava Cockerell, rufulina Cockerell, rufosuffusa Cockerell, melanura Cockerell, asarna Cockerell, capiticola Cockerell, heteroscopa Cockerell, capiticola Cockerell and chrysognatha Cockerell are M. frontalis Smith; boswendica Cockerell, rubrociliata Pasteels and rufisetosa Pasteels are M. pilosella Friese; apiformis Smith is M. ungulata Smith; stellarum Cockerell, laticeps Friese, malangensis mamalapia Pasteels, obesa Pasteels and ovatomaculata Pasteels are M. malangensis Friese; flavibasis Cockerell,heterotricha Cockerell, candidicauda Cockerell, candidigena Cockerell, candidicauda spinarum Cockerell, neliCockerell, albofilosa Cockerell, discretula Cockerell, rubeola Pasteels and meesi Pasteels are M. chrysopogon Vachal; lydenburgiana Strand, aliceae Cockerell, pretoriaensis Pasteels and pycnocephala Pasteels are M. semierma Vachal; benitocola Strand and granulicauda Cockerell are M. curtula Gerstaecker; and spatulicornis Pasteels is M. edwardsi Friese. Brief descriptions are provided for all the species, as are their distributions in southern Africa, known host plants and parasites. Keys for the identification of the species are also given.
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5

EARDLEY, CONNAL. "A taxonomic revision of the southern African species of the subgenus Creightonella Cockerell (Apoidea: Megachilidae: Megachile Latreille)." Zootaxa 3159, no. 1 (January 13, 2012): 1. http://dx.doi.org/10.11646/zootaxa.3159.1.1.

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The southern African species of Megachile (Creightonella) are revised. Twelve valid species are recognised, one of whichis new, Megachile serrula sp. n., and four are only known from the female. Ten new synonymies are recorded. Megachilecognata var. claripennis Friese, Megachile natalensis Friese are synonymized with Megachile cognata Smith. Megachileconsanguinea zamelaena Cockerell is synonymized with Megachile ianthoptera Smith. Megachile hopilitis Vachal,Megachile vanderysti Cockerell, Megachile triangulifera kivuicola Cockerell, and Megachile heptadonta Cockerell aresynonymized with Megachile angulata Smith. Four new lectotypes were designated; they are Megachile natalensis Friese,M. discolor Smith, M. cornigera Friese and M. braunsiana Friese. The nomenclatorial history of each species is documented, diagnoses are given, food plants are recorded and distribution maps are provided.
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6

Engel, Michael S., and Donald Burton Baker. "A remarkable new leaf-cutter bee from Thailand (Hymenoptera: Magachilidae)." Beiträge zur Entomologie = Contributions to Entomology 56, no. 1 (August 15, 2006): 69–74. http://dx.doi.org/10.21248/contrib.entomol.56.1.69-74.

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Die bemerkenswerte und als neu erkannte Blattschneiderbienen-Art, Megachile trichorhytisma Engel sp. n. wird beschrieben. Die Beschreibung wird ergänzt durch Abbildungen verschiedener Ansichten des Habitus und der Genitalien zweier Männchen, die im nördlichen Thailand gesammelt wurden. Die Art wird in die neue Untergattung Aethomegachile Engel & Baker subgen. n. gestellt, die von bisher bekannten Abstammungslinien von Megachile unterschieden wird.StichwörterApoidea, Anthophila, Megachilinae, Megachilini, Southeast Asia, taxonomy.Nomenklatorische HandlungenAethomegachile Engel & Baker, 2006 (Megachile), sgen. n.trichorhytisma Engel, 2006 (Megachile (Aethomegachile)), spec. n.
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7

Orr, Michael C., Zachary M. Portman, and Terry L. Griswold. "Megachile (Megachile) montivaga (Hymenoptera: Megachilidae) nesting in live thistle (Asteraceae: Cirsium)." Journal of Melittology, no. 48 (March 25, 2015): 1. http://dx.doi.org/10.17161/jom.v0i48.4847.

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Although Megachile Latreille (leafcutter bees) are well known for their diverse nesting habits, records of the genus nesting in live plants are rare. Here, we report the widespread Megachile (Megachile) montivaga Cresson nesting in live thistle (Cirsium neomexicanum Gray), the first explicit record of this behavior in the Nearctic.
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8

Maharramov, M. M., A. V. Fateryga, and M. Yu Proshchalykin. "Megachilid bees (Hymenoptera: Megachilidae) of the Nakhchivan Autonomous Republic of Azerbaijan: tribes Lithurgini, Dioxyini, and Megachilini." Far Eastern entomologist 428 (April 2, 2021): 12–24. http://dx.doi.org/10.25221/fee.428.3.

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The data on 38 species of megachilid bees in the tribes Lithurgini, Dioxyini, and Megachilini collected in the Nakhchivan Autonomous Republic of Azerbaijan mainly in 2018–2020 are given. Seventeen species are new to Azerbaijan, four other species are new to the Nakhchivan Autonomous Republic and as a result of the present investigation the fauna of Azerbaijan currently numbers 160 species of the family Megachilidae. A mosaic gynandro¬morph of Megachile albisecta (Klug, 1817) is also described.
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9

Kumari, Priyanka, Neelima R. Kumar, and Avtar K. Sidhu. "Taxonomic studies on subgenus Pseudomegachile Friese under genus Megachile Latreille (Hymenoptera) from Sutlej basin plains (India)." Journal of Applied and Natural Science 12, no. 2 (May 22, 2020): 146–55. http://dx.doi.org/10.31018/jans.vi.2259.

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Genus Megachile forms an integral part of the ecosystem as its members are important pollinators of both wild and cultivated flora. Surveying on a large scale helps in gathering a lot of information about their spatial and temporal data. This also helps in studying their biodiversity and taxonomy. Three species of solitary bees under subgenus Pseudomegachile Friese of the genus Megachile Latreille collected from Sutlej basin plains of India have been studied and described taxonomically. Megachile (Pseudomegachile) creusa (Bingham), M. (P.) ramakrishnae Cockerell and M. (P.) elfrona (Cameron) with 21 examples are described including their morphological characters, taxonomically important characters, zoogeographic records, floral associations along with genitalic and sternal plates. Since most of the areas included in the study have been surveyed first time for Megachile study, these are new records for the studied area.
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10

RAW, ANTHONY. "Nomenclatural changes in leafcutter bees of the Americas: Megachile Latreille 1802 (Hymenoptera; Megachilidae)." Zootaxa 766, no. 1 (December 14, 2004): 1. http://dx.doi.org/10.11646/zootaxa.766.1.1.

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Five species of Megachile are placed in their correct subgenera, raising the total number of correctly placed species of this genus to 519 in the Americas. Megachile (Dasymegachile) mitchelli new name is proposed for Cressoniella (Chaetochile) golbachi Schwimmer, in Mitchell, 1980.
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11

Le Goff, Gérard. "Nidification dans des cavités pierreuses de deux Megachilidae de Grèce (Epire) : Hoplitis (Hoplitis) fabrei van der Zanden, 1987 et Megachile (Chalicodoma) manicata Giraud, 1861 (Hymenoptera, Megachilidae, Osmiini et Megachilini)." Osmia 5 (2012): 3–4. http://dx.doi.org/10.47446/osmia5.2.

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Nesting in stony cavities of two Greek Megachilidae (Epirus): Hoplitis (Hoplitis) fabrei van der Zanden, 1987 and Megachile (Chalicodoma) manicata Giraud, 1861 (Hymenoptera, Megachilidae, Osmiini and Megachilini). - In this note are described two nests built in little stony cavities by two Megachilidae in Greece: Hoplitis fabrei and Megachile manicata.
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12

SARDAR, SAYAN, ANANDHAN RAMESHKUMAR, and SARFRAZUL ISLAM KAZMI. "First report of Megachile (Callomegachile) sculpturalis Smith, 1853 (Apoidea: Megachilidae) from India." Journal of Insect Biodiversity 23, no. 2 (April 16, 2021): 43–49. http://dx.doi.org/10.12976/jib/2021.23.2.2.

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The giant resin bee, Megachile (Callomegachile) sculpturalis (Smith, 1853) is an invasive solitary bee that has spread all over North America and Europe in the last decade. Although native to Asia, not much is known about its distribution in this continent. In the present study, Megachile (Callomegachile) sculpturalis is reported for the first time from India. Related information about this species regarding its nesting habits, floral association, and impact in introduced range obtained through bibliographical research along with the possible dispersal route after introduction in South Eastern Asia is provided. The checklist of Megachile (Callomegachile) in India is also provided. Key words: Pollinator, giant resin bee, range extension, Northeast India
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13

Chatthanabun, Nontawat, John S. Ascher, Nantasak Pinkaew, Chawatat Thanoosing, Prapun Traiyasut, and Natapot Warrit. "Resin bees of genus Megachile, subgenera Callomegachile and Carinula (Hymenoptera, Megachilidae) from Thailand with description of a new species." ZooKeys 997 (November 25, 2020): 95–144. http://dx.doi.org/10.3897/zookeys.997.34935.

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Resin bees of the genus Megachile subgenus Callomegachile sensu lato (Hymenoptera; Megachilidae) from Thailand are reviewed. The 14 species treated include those described or revised in the subgenus Alocanthedon, a junior synonym of Callomegachile (three species), and in Carinula (one species). One new species is described, Megachile chiangmaiensis Chatthanabun and Warrit, sp. nov. The replacement name Megachile parornata Chatthanabun, Warrit and Ascher, nom. nov., is proposed for M. gigas Wu (not Schrottky), which is recorded for the first time outside China. For each species, maps and full label data for the examined material documenting occurrences in Thailand are provided. In addition, global ranges, floral associations, and other life history data are summarized and a key to the Thai species is provided for females.
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14

NIU, ZE-QING, YAN-RU WU, and CHAO-DONG ZHU. "A review of Megachile (Chelostomoda) Michener (Megachilidae: Megachilini) known from China with the description of a new species." Zootaxa 3267, no. 1 (April 12, 2012): 55. http://dx.doi.org/10.11646/zootaxa.3267.1.3.

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The Chinese species of Megachile (Chelostomoda) Michener, 1962, are treated in this paper. Megachile (C.) guangxiensesp. nov. is described and illustrated. A checklist of the known Chinese species, distribution records, and an updated iden-tification key are provided. The type specimens of M. guangxiense are deposited in the Insect Collection of the Institute of Zoology, Chinese Academy of Sciences, Beijing, China (IZCAS, Beijing).
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GONZALEZ, VICTOR H., and TERRY L. GRISWOLD. "A review of the North and Central American Megachile subgenus Argyropile Mitchell (Hymenoptera: Megachilidae)." Zootaxa 1461, no. 1 (April 30, 2007): 1–14. http://dx.doi.org/10.11646/zootaxa.1461.1.1.

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We provide a synopsis of Megachile subgenus Argyropile, describe a new species from Mexico and Guatemala, present descriptions for previously unknown males, and explore the internal phylogeny of the group. We also provide an illustrated key in Spanish and English to all of its species. Seven species, including Megachile longuisetosa Gonzalez & Griswold, new species, are recognized herein. Females of M. longuisetosa can be separated from other species of Argyropile by the combination of the following characters: mid basitarsus with anterodistal margin strongly, acutely projected, mesoscutal-mesoscutellar suture with distinct pubescent fascia, and apical margin of sixth sternum abruptly bent dorsally and males by the sixth tergum with preapical carina and apical margin entire, fifth and sixth sterna with simple, unmodified setae, and apex of gonoforceps with long setae. The males of Megachile sabinensis and M. rossi, are described and illustrated for the first time. Megachile asterae and M. tulariana are considered as new synonyms of M. parallela. In a cladistic analysis, two most parsimonious trees were obtained for Argyropile, from 13 morphological characters. Two clades were clearly differentiated: one clade includes M. sabinensis and M. subparallela, while the other one includes the remaining species. The phylogenetic relationship of the new species and the biology of the subgenus are discussed.
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Kumari, Priyanka, Neelima R. Kumar, Avtar K. Sidhu, and Kailash Chandra. "Taxonomic studies on species belonging to subgenus Eutricharaea Thomson of the genus Megachile (Hymenoptera: Megachilidae)." Journal of Applied and Natural Science 11, no. 3 (September 10, 2019): 612–18. http://dx.doi.org/10.31018/jans.v11i3.2125.

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Solitary bees belonging to genus Megachile are important pollinators very crucial for the wellbeing of the ecosystem. Large scale surveying will return a wealth of spatio-temporal species data which is critical for systematics, taxonomy and conservation of biodiversity. Detailed taxonomic characterization of solitary bee species belonging to subgenus Eutricharaea collected from the Sutlej basin of Indian northern plains is being described. A total of four species viz. Megachile hera Bingham, M. femorata Smith, M. vera Cameron and M. argentata Cameron belonging to subgenus Eutricharaea with 18 examples were studied. Detailed morphological descriptions, zoogeographic records, specimen examined, floral associations with illustrations and morphological measurements have been provided. Various areas finalised from different parts of Sutlej basin of northern plains were surveyed for the first time for the documentation of Megachile fauna. Hence, all these four species are first records for the studied area.
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Kumari, Priyanka, and Neelima R. Kumar. "Description of a new species of Neocressionella Gupta (Hymenoptera: Megachilidae) from North western India." Journal of Applied and Natural Science 6, no. 2 (December 1, 2014): 779–81. http://dx.doi.org/10.31018/jans.v6i2.535.

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A new species, Megachile (Neocressionella) patialensis is described from Patiala, India. Full description of male with their floral relationship, morphological measurements and specimens examined is provided. Knowledge of the life history and nesting habits of Megachile bees will pave the way for their manipulation so that these can be used to increase pollination. Documentation of the genera and species will increase our knowledge of the biodiversity of these bees in the study area.
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Le Féon, Violette, and Benoît Geslin. "Écologie et distribution de l’abeille originaire d’Asie Megachile sculpturalis Smith 1853 (Apoidea - Megachilidae - Megachilini) : un état des connaissances dix ans après sa première observation en Europe." Osmia 7 (2018): 31–39. http://dx.doi.org/10.47446/osmia7.6.

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Ecology and distribution of the Asian bee Megachile sculpturalis Smith 1853 (Apoidea - Megachilidae - Megachilini): a state of knowledge ten years after its first observation in Europe. - Megachile sculpturalis (the Giant Resin Bee) is a bee species native to East Asia that has been observed for the first time in Europe in 2008 in Allauch near Marseille (France). In the USA, it has been reported in 1994 and has since then rapidly expanded through North America. We here provide a review on its ecology and its geographical distribution.
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Gonzalez, Victor H., Diego A. Guevara, Joanna Jaramillo-Silva, and Rodulfo Ospina. "Discovery of Megachile (Pseudomegachile) lanata (Fabricius, 1775) (Hymenoptera, Megachilidae) in Colombia, an adventive bee species from the Old World." Check List 15, no. 1 (January 18, 2019): 45–48. http://dx.doi.org/10.15560/15.1.45.

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We record for the first time for Colombia Megachile (Pseudomegachile) lanata (Fabricius, 1775), a bee species from Southeast Asia. This is the first record of an adventive bee species for the country besides Apis mellifera Linnaeus, 1758, the European honey bee. Megachile lanata appears to have arrived to the Caribbean coast of Colombia nearly half a century ago, reaching the Orinoquia region recently. We provide comments on diagnostic features to facilitate the recognition of this bee species and discuss its possible establishment in Colombia.
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20

DURANTE, SILVANA, and NORA CABRERA. "Cladistic analysis of Megachile (Chrysosarus) Mitchell and revalidation of Megachile (Dactylomegachile) Mitchell (Hymenoptera, Megachilidae)." Zootaxa 2284, no. 1 (November 9, 2009): 48–62. http://dx.doi.org/10.11646/zootaxa.2284.1.4.

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Megachile is the largest genus of the family Megachilidae, being widely distributed throughout temperate and tropical areas of the world. The validity of M. (Chrysosarus) and M. (Dactylomegachile) was investigated based on a cladistic analysis of 63 characters of adult morphology of 14 taxa. The single most parsimonious tree (181 steps, CI=0.60; RI=0.63) resulting from an implied weighting analysis recovered M. (Chrysosarus) and M. (Dactylomegachile) as monophyletic groups. Both subgenera are redescribed and the value of the most frequently used characters in the taxonomy of Megachile is discussed.
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Vereecken, Nicolas J., and Éric Barbier. "Premières données sur la présence de l’abeille asiatique Megachile (Callomegachile) sculpturalis Smith (Hymenoptera, Megachilidae) en Europe." Osmia 3 (2009): 4–6. http://dx.doi.org/10.47446/osmia3.3.

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TORRETTA, JUAN PABLO, and SILVANA PATRICIA DURANTE. "Nesting ecology of Megachile (Sayapis) mendozana Cockerell and its synonymy with Megachile (Sayapis) santiaguensis Durante (Hymenoptera: Megachilidae)." Zootaxa 3008, no. 1 (August 30, 2011): 63. http://dx.doi.org/10.11646/zootaxa.3008.1.3.

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We synonymized M. santiaguensis Durante with M. mendozana Cockerell based on individual siblings of different sex obtained from a trap-nest. Similarity in morphology between individuals of both sexes of M. mendozana, as well as the overlapping distribution, provides further evidence to support this synonymy. Also, we report aspects of nesting ecology and analyze the pollen in cell provisions and/or feces of 11 samples of 4 different trap-nests. Additionally, floral host, associated organisms, and new geographical records are provided.
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RAW, ANTHONY. "An annotated catalogue of the leafcutter and mason bees (genus Megachile) of the Neotropics." Zootaxa 1601, no. 1 (September 28, 2007): 1–127. http://dx.doi.org/10.11646/zootaxa.1601.1.1.

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Megachile is treated here as a single genus combining Megachile with Chalicodoma. In the Americas the genus occurs from Alaska and Canada to southern Chile and Argentina, and is well represented in the tropics. Despite their economic and ecological importance as pollinators, the biology of few species has been studied in detail largely because they are difficult to identify. The 419 species in the catalogue are allocated to 28 subgenera (including 10 Old World species introduced to the region and one species dubiously recorded from the New World). Summaries of information on each species’ classification, biology and geographical distribution are given.
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Rocha-Filho, Léo Correia, Aline C. Martins, and Paola Marchi. "Notes on a nest of Megachile (Moureapis) apicipennis Schrottky (Megachilidae) constructed in an abandoned gallery of Xylocopa Frontalis (Olivier) (Apidae)." Sociobiology 64, no. 4 (December 27, 2017): 488. http://dx.doi.org/10.13102/sociobiology.v64i4.1928.

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Notes on a nest of the leafcutting bee Megachile (Moureapis) apicipennis Schrottky (Megachilidae) found in an abandoned gallery excavated by the carpenter bee Xylocopa frontalis (Olivier) (Apidae) are presented. A total of four nests were found at the end of the gallery. Brood cells were lined with imbricate pieces of leaves of Centrosema virginianum (L.) Benth. (Fabaceae). Four males and two females of M. apicipennis emerged as well as individuals of three species of natural enemies: Coelioxys otomita Cresson (Megachilidae), Brachymeria paraguayensis (Brèthes) and Melittobia australica Girault (Eulophidae). Our results were similar to the data obtained from other species of Megachile (Moureapis) Raw.
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Oliveira, Priscila Soares, and Rodrigo Barbosa Gonçalves. "Trap-nesting bees and wasps (Hymenoptera, Aculeata) in a Semidecidual Seasonal Forest fragment, southern Brazil." Papéis Avulsos de Zoologia (São Paulo) 57, no. 13 (March 16, 2017): 149. http://dx.doi.org/10.11606/0031-1049.2017.57.13.

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Trap-nesting bee and wasp inventories are common in Brazil but many phytophysiognomies are still poorly studied. The main objective of this study is to survey trap-nesting bees and wasps in a Semidecidual Seasonal Forest fragment. Also, we test the differences on nesting between interior and edge transects. A sum of 1,500 trap nests was made with bamboo cane internodes and two consecutive years were monitored. In the first year 46 nests were occupied by Pachodynerus grandis (19 nests), Pachodynerus guadulpensis (19), Centris analis (two), and Centris tarsata, Megachile fiebrigi, Megachile guaranitica, Megachile susurrans, Trypoxylon sp and Zethus smithii with one nest each. No statistical differences were found between interior and edge transects for richness and occupation rate, but the species composition was different. In the second year 39 nests were occupied by four species, three previously recorded, C. analis (seven nests), P. guadulpensis and P. grandis (six nests each), plus Monobia angulosa with 15 nests. Parasitoids from four families and one cleptoparasite were recorded and the mortality rate was higher in bees than in wasps. These findings reinforce the notion that trap nests assemblages from different studies are not directly comparable for richness and composition.
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Lu, Huanhuan, Bo He, Youjin Hao, Zeyang Zhou, Chengyong Su, and Dunyuan Huang. "Comparative Mitogenomic Analysis of Two Cuckoo Bees (Apoidea: Anthophila: Megachilidae) with Phylogenetic Implications." Insects 12, no. 1 (January 5, 2021): 29. http://dx.doi.org/10.3390/insects12010029.

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Bees (Hymenoptera, Apoidea and Anthophila) are distributed worldwide and considered the primary pollinators of angiosperm. Megachilidae is one of the largest families of Anthophila. In this study, two complete mitogenomes of cuckoo bees in Megachilidae, namely Coelioxys fenestrata and Euaspis polynesia, were amplified and sequenced, with a length of 17,004 bp (C. fenestrata) and 17,682 bp (E. polynesia). The obtained results show that 37 mitogenomic genes and one putative control region were conserved within Hymenoptera. Truncated stop codon T was found in the cox3 gene of E. polynesia. The secondary structure of small (rrnS) and large (rrnL) rRNA subunits contained three domains (28 helices) and five domains (44 helices) conserved within Hymenoptera, respectively. Compared with ancestral gene order, gene rearrangement events included local inversion and gene shuffling. In order to reveal the phylogenetic position of cuckoo bees, we performed phylogenetic analysis. The results supported that all families of Anthophila were monophyletic, the tribe-level relationship of Megachilidae was Osmiini + (Anthidiini + Megachilini) and Coelioxys fenestrata was clustered to the Megachile genus, which was more closely related to Megachile sculpturalis and Megachile strupigera than Euaspis polynesia.
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Sheffield, Cory Silas, and Julio A. Genaro. "A new species of Megachile (Litomegachile) from Cuba, the Antilles (Hymenoptera: Megachilidae)." Journal of Melittology, no. 19 (October 7, 2013): 1. http://dx.doi.org/10.17161/jom.v0i19.4564.

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A new species of leafcutter bee, Megachile (Litomegachile) droegei Sheffield & Genaro, new species, is described from Guantanamo Bay, Cuba. Megachile droegei is one of 12 species in this New World subgenus, and is part of the M. brevis species group (with M. brevis Say, M. onobrychidis Cockerell, and M. pseudobrevis Mitchell). This is the first record of the subgenus Litomegachile from the Antilles. A diagnosis for distinguishing this species from other species of Litomegachile, and full descriptions of the female and male with illustrations are provided. In addition, the male of M. pankus Bzdyk is described, and M. cleomis Cockerell is recognized as a valid species (new status) based on 1.45% divergence in COI (cytochrome c oxidase, subunit 1) from M. texana Cresson, and eastern (M. texana) /western (M. cleomis) distributions. Based on morphological data and COI sequence data, four distinct species groups are recognized within Litomegachile. Putative synapomorphies that support the monophyly of these groups are also discussed.RESUMEN: Se describe una especie nueva de abeja cortadora de hojas, Megachile (Litomegachile) droegei, para la Bahía de Guantánamo, Cuba. Megachile droegei es una de las 12 especies pertenecientes al subgénero del Nuevo Mundo, Litomegachile y es parte del grupo de especies de M. brevis (junto a M. brevis Say, M. onobrychidis Cockerell y M. pseudobrevis Mitchell). Este constituye el primer registro del subgénero Litomegachile para Las Antillas. Se provee una diagnosis para separar a la especie nueva del resto de las especies del mismo subgénero, asícomo descripciones completas e ilustraciones de ambos sexos. Además, se describe el macho de M. pankus Bzdyk y se reconoce M. cleomis Cockerell como una especie válida (nuevo estado) basado en una divergencia del COI (citocromo coxidasa, subunidad 1) de 1,45 %, respecto a las distribuciones con M. texana Cresson, y poblaciones del Este (M. texana) /Oeste (M. cleomis). Basado en datos morfológicos y datos de la secuencia de COI, cuatro grupos de especies son reconocidos en Litomegachile. Tambiense discuten posibles sinapormofias que apoyan la monofilia de estos grupos.
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Roig Alsina, Arturo. "The subgenus Chelostomoides in Argentina (Hymenoptera: Megachilidae: Megachile)." Revista de la Sociedad Entomológica Argentina 78, no. 3 (September 26, 2019): 22–25. http://dx.doi.org/10.25085/rsea.780304.

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Teixeira, Frederico Machado, Thaís Araújo Cordeiro Schwartz, and Maria Cristina Gaglianone. "Biologia da nidificação de Megachile (Moureapis) benigna Mitchell." EntomoBrasilis 4, no. 3 (November 26, 2011): 92–99. http://dx.doi.org/10.12741/ebrasilis.v4i3.140.

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Este trabalho descreve aspectos da biologia, ecologia e arquitetura de ninhos de Megachile (Moureapis) benigna Mitchell em ninhos-armadilha (NA). Os NA foram instalados em cinco fragmentos florestais de 2,1 a 920ha, e duas áreas antrópicas (pasto e plantação). Foram utilizados gomos de bambu (NB) e tubos de cartolina preta inseridos em placas de madeira (NC) para atrair fêmeas em nidificação. As abelhas ocuparam 17 NA (12 NB - 9 a 21mm de diâmetro - e 5 NC - 8 a 10mm) em quatro fragmentos florestais, construindo 26 ninhos (um a seis por NB). Nenhum ninho foi fundado nas áreas antrópicas. Não houve diferença entre o tamanho dos ninhos construídos em NB e NC (t= 0,31; p= 0,763; gl= 25). O número de células variou de 1 a 13 por ninho, sendo mais largas em NB do que em NC (t= 2,26; p= 0,033; gl= 25). A atividade de construção ocorreu durante o período chuvoso (outubro a março), sem correlação com parâmetros climáticos. A emergência (1 a 12 por ninho) foi correlacionada com a umidade (t= 3,013; p= 0,006). O tempo de emergência variou de 1 a 141 dias a partir da coleta do ninho. A proporção sexual foi de 1,8:1 macho/fêmea, diferindo de 1:1 (Χ2= 9,39; p<0,002). Ninhos foram parasitados por Coelioxys otomita Cresson e outras duas espécies de Coelioxys. A espécie demonstra plasticidade na utilização do substrato, com preferência por cavidades com diâmetros maiores que 9mm. Existe uma possível dependência da espécie a ambientes florestais, uma vez que a maior ocorrência de nidificação se deu em áreas de floresta em estágio avançado de regeneração. Nesting Biology of Megachile (Moureapis) benigna Mitchell Abstract. This paper describes the biology, ecology and nest architecture of Megachile (Moureapis) benigna Mitchell in trap nests (NA). The NA were installed in five forest fragments of 2.1 to 920ha, and two disturbed areas (pasture and plantation). We used bamboo canes (NB) and black cardboard tubes placed on wooden boards (NC) to attract nesting females. Bees occupied 17 NA (12 NB - 9 to 21mm in diameter - and 5 NC - 8 to 10mm) in four forest fragments forming 26 nests (one to six nests per NB). No nest was founded in disturbed areas. There was no difference between NB and NC related to the size of formed nests (t= 0.31, p= 0.763, df= 25). The number of cells ranged from 1 to 13 per nest, being larger in NB than in NC (t= 2.26, p= 0.033, df= 25). The construction activity occurred during the rainy season (October to March), with no correlation to climate parameters. Emergence (1 to 12 per nest) was correlated with humidity (t= 3.013, p= 0.006). Time to adult emergence varied from 1 to 141 days. The sex ratio was 1.8:1 male/female, differing from 1:1 (Χ2= 9.39, p<0.002). The parasites were Coelioxys otomita Cresson and two other species of Coelioxys. The species demonstrates plasticity in nesting substrate, and preference for cavities with diameters larger than 9mm. There is a possible dependence of the species to forest environments, since the higher occurrence of nesting was in forest areas in advanced stages of regeneration.
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Gonzalez, Victor H. "Taxonomic comments on Megachile subgenus Chrysosarus (Hymenoptera: Megachilidae)." Journal of Melittology, no. 5 (February 18, 2013): 1. http://dx.doi.org/10.17161/jom.v0i5.4451.

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Comments on the phylogenetic relationships and taxonomy of American leafcutter bees of the genus Megachile subgenus Chrysosarus are provided. The South American subgenera Austrosarus Raw, Stelodides Moure, and Zonomegachile Mitchell are newly synonymized under Chrysosarus Mitchell.
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31

Roig Alsina, Arturo. "Joergensenella, a new subgenus of Neotropical Megachile (Hymenoptera: Megachilidae), with a key to Argentinean Megachile with specialized facial pollen-harvesting hairs." Revista del Museo Argentino de Ciencias Naturales 22 (2020): 21–46. http://dx.doi.org/10.22179/revmacn.22.663.

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32

Starr, Christopher K. "A nesting aggregation of the solitary bee Megachile atrata (Hymenoptera: Megachilidae) in the Philippines." Journal of Melittology, no. 68 (April 20, 2017): 1–5. http://dx.doi.org/10.17161/jom.v0i68.6467.

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A nesting aggregation of Megachile (Creightonella) atrata Smith in the Philippines comprised almost 300 active nests. The bees in rapid flight resemble the hornet Vespa tropica Linnaeus. The nest structure is similar to that reported for M. (C.) frontalis (Fabricius) in New Guinea.
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Fateryga, A. V., and M. Yu Proshchalykin. "New records of megachilid bees (Hymenoptera: Megachilidae) from the North Caucasus and the south of European Russia." Caucasian Entomological Bulletin 16, no. 2 (2020): 225–331. http://dx.doi.org/10.23885/181433262020162-225331.

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New data on 22 species of bees of the family Megachilidae from the North Caucasus and the south of European Russia are reported. Six species are new to Russia: Hoplitis curvipes (Morawitz, 1871), Osmia cinerea Warncke, 1988, O. ligurica Morawitz, 1868, O. cyanoxantha Pérez, 1879, Protosmia glutinosa (Giraud, 1871), and Coelioxys mielbergi Morawitz, 1880. Hoplitis turcestanica (Dalla Torre, 1896), sp. resurr. is treated as a distinct species, not a junior synonym of H. caularis (Morawitz, 1875). Megachile albocristata Smith, 1853 and M. alborufa Friese, 1911 are listed instead of previously recorded M. lefebvrei (Lepeletier de Saint-Fargeau, 1841) and M. pyrenaica (Lepeletier de Saint-Fargeau, 1841), respectively. Fourteen new regional records are reported: seven species are new to the North Caucasus, five ones are new to the south of European Russia, and two species are new to the European part of Russia as a whole. The numbers of megachilid bee species currently known in Russia, the North Caucasus, and the south of European Russia are 217, 130, and 71, respectively. The lectotype of Osmia proxima Morawitz, 1875 is designated.
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Richards, K. W. "Ovarian development in the alfalfa leafcutter bee,Megachile rotundata." Journal of Apicultural Research 33, no. 4 (January 1994): 199–203. http://dx.doi.org/10.1080/00218839.1994.11100871.

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dos Santos, Adauto Alex, Daniele Parizotto, Clemens Schlindwein, and Celso Feitosa Martins. "Nesting biology and flower preferences of Megachile (Sayapis) zaptlana." Journal of Apicultural Research 59, no. 4 (January 9, 2020): 609–25. http://dx.doi.org/10.1080/00218839.2019.1703084.

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36

Roig-Alsina, A., and J. P. Torretta. "The leucografa species group of Megachile (Chrysosarus) (Hymenoptera: Megachilidae)." Journal of Natural History 55, no. 7-8 (February 16, 2021): 457–70. http://dx.doi.org/10.1080/00222933.2021.1905097.

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Pitts-Singer, Theresa L. "Olfactory Response of Megachilid Bees, Osmia lignaria, Megachile rotundata, and M. pugnata, to Individual Cues from Old Nest Cavities." Environmental Entomology 36, no. 2 (April 1, 2007): 402–8. http://dx.doi.org/10.1093/ee/36.2.402.

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38

Le Féon, Violette, David Genoud, and Benoît Geslin. "Actualisation des connaissances sur l’abeille Megachile sculpturalis SMITH, 1853 en France et en Europe (Hymenoptera : Megachilidae)." Osmia 9 (July 12, 2021): 25–36. http://dx.doi.org/10.47446/osmia9.4.

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Update of knowledge on the bee Megachile sculpturalis SMITH, 1853 in France and Europe (Hymenoptera: Megachilidae). - Megachile sculpturalis is a bee native to eastern Asia that is now also present in North America and Europe. This article follows the first synthesis on the ecology and geographical distribution in Europe of this species published in this same journal in 2018 and updates their knowledge. Megachile sculpturalis has been observed in 13 European countries. Its range now extends from the French Atlantic coast in the west to the Crimean Peninsula in the east. It has also been recorded on two Mediterranean islands, Elba (Italy) and Mallorca (Spain). In France, between 2008 and 2020, it was observed in 259 communes in 46 departments. The most northerly French observation was made in 2020 near Reims in the Marne department. Concerning feeding behaviour, new pollen analyses corroborate the results of previous ones which suggested a polylectic diet with a marked preference for shrubs of Asian origin (especially Sophora japonica and Ligustrum sp.). Regarding nesting behaviour, M. sculpturalis makes extensive use of bee hotels. However, a range of diameters (holes in logs or sections of plant stems) between 4 and 8 mm is suitable for the main native species likely to nest in the hotels, while limiting the establishment of M. sculpturalis. New observations have confirmed the tendency of M. sculpturalis to empty the nests of other species in order to establish its own larval cells. In addition, in Switzerland, a female was observed killing a bee of the genus Heriades. Such events, if frequent, could have negative impacts on native bees, but data are currently insufficient to quantify these impacts on populations.
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Rahayu, Sayekti Kurnia, Supriyadi Supriyadi, Supriyono Supriyono, Retno Wijayanti, Retno Bandriyati Arni Putri, and Retno Bandriyati Arni Putri. "Keanekaragaman serangga pengunjung bunga pada tanaman tumpang sari kedelai dengan tanaman orok-orok (Crotalaria juncea)." Jurnal Entomologi Indonesia 15, no. 1 (October 10, 2018): 23. http://dx.doi.org/10.5994/jei.15.1.23.

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Tumbuhan berbunga dapat meningkatkan populasi serangga polinator, yang sekaligus berperan penting dalam meningkatkan hasil tanaman. Tujuan penelitian ini untuk mengetahui pengaruh tumpang sari Crotalaria juncea dengan kedelai terhadap keanekaragaman serangga pengunjung bunga dan polinator alami terhadap hasil kedelai. Penelitian dirancang pada petak tunggal, dengan perlakuan: tanpa C. juncea (kontrol), penanaman C. juncea mengelilingi lahan kedelai, penanaman C. juncea setiap 5 baris kedelai, dan penanaman C. juncea setiap 10 baris kedelai. Pengaruh polinator alami terhadap hasil kedelai diamati melalui penyungkupan tanaman dengan jaring serangga. Hasil penelitian menunjukan, bahwa penanaman C. juncea dapat meningkatkan keanekaragaman serangga pengunjung bunga. Indeks keanekaragaman tertinggi 2,37 tercatat pada petak penanaman C. juncea setiap 10 baris kedelai. Keanekaragaman serangga pengunjung bunga terbanyak, yaitu 18 spesies ditemukan pada perlakuan penanaman C. juncea setiap 5 baris kedelai. Serangga pengunjung bunga yang dominan adalah Coccinella transversalis, Apis mellifera, Xylocopa virginica, Megachile parientina, Megachile relativa, Ropalidia fasciata, dan Vespa sp. Polinator alami (tanpa sungkup) dapat meningkatkan jumlah polong 30,11%, jumlah biji 44,63%, dan berat biji per tanaman 15,44% sehingga berperanan penting pada hasil kedelai.
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Sheffield, Cory. "A new species of Megachile Latreille subgenus Megachiloides (Hymenoptera, Megachilidae)." ZooKeys 283 (April 3, 2013): 43–58. http://dx.doi.org/10.3897/zookeys.283.4674.

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41

Wilson, Elisabeth S., Claire E. Murphy, Joseph P. Rinehart, George Yocum, and Julia H. Bowsher. "Microclimate Temperatures Impact Nesting Preference in Megachile rotundata (Hymenoptera: Megachilidae)." Environmental Entomology 49, no. 2 (February 28, 2020): 296–303. http://dx.doi.org/10.1093/ee/nvaa012.

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Abstract The temperature of the nest influences fitness in cavity-nesting bees. Females may choose nest cavities that mitigate their offspring’s exposure to stressful temperatures. This study aims to understand how cavity temperature impacts the nesting preference of the solitary bee Megachile rotundata (Fabricius) under field conditions. We designed and 3D printed nest boxes that measured the temperatures of 432 cavities. Nest boxes were four-sided with cavity entrances facing northeast, northwest, southeast, and southwest. Nest boxes were placed along an alfalfa field in Fargo, ND and were observed daily for completed nests. Our study found that cavity temperature varied by direction the cavity faced and by the position of the cavity within the nest box. The southwest sides recorded the highest maximum temperatures while the northeast sides recorded the lowest maximum temperatures. Nesting females filled cavities on the north-facing sides faster than cavities on the south-facing sides. The bees preferred to nest in cavities with lower average temperatures during foraging hours, and cavities that faced to the north. The direction the cavity faced was associated with the number of offspring per nest. The southwest-facing cavities had fewer offspring than nests on the northeast side. Our study indicates that the nesting box acts as a microclimate, with temperature varying by position and direction of the cavity. Variation in cavity temperature affected where females chose to nest, but not their reproductive investment.
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Alfken, J. D. "Beitrag zur Kenntnis der Megachile-Arten von Japan (Hym., Apid.)." Berliner entomologische Zeitschrift 1931, no. 2-3 (April 19, 2008): 167–76. http://dx.doi.org/10.1002/mmnd.193119310205.

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43

Amala, U., and T. M. Shivalingaswamy. "Trap-Nest Diameter Preference of Megachile lanata (Hymenoptera: Megachilidae)1." Journal of Entomological Science 53, no. 1 (January 2018): 96–98. http://dx.doi.org/10.18474/jes17-88.1.

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44

Buschini, MLT, J. Rigon, and J. Cordeiro. "Plants used by Megachile (Moureapis) sp. (Hymenoptera: Megachilidae) in the provisioning of their nests." Brazilian Journal of Biology 69, no. 4 (November 2009): 1187–94. http://dx.doi.org/10.1590/s1519-69842009000500025.

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This study was carried out in the Parque Municipal das Araucárias, located in the municipality of Guarapuava (PR) from May (06) to April (07). Its aim was to investigate which plants are used by Megachile (Moureapis) sp in provisioning of larvae, and to verify if this bee is oligolectic or polilectic. The collection of the plants was carried out during the bees activity period and in an area covering a 500 m radius from where the nests had been built. A sample of pollens from founded nests and from flowers was used to make permanent slides using the acetolysis method and with the aid of a light microscope, all pollen grains were examined in order to identify the plants used by this bee. A total of 80 pollen grain slides, from 16 nests were analysed. Although 99 plants were collected close to the bee's nesting site, in nest slides, the pollen of Ludwigia peruviana (36%) and Ludwigia sericea (63.6%) (Onagraceae) accounted for 99.6% of the total amount of pollen collected. With these results we are able to conclude that Megachile (Moureapis) sp is an oligolectic species and that these plants species are their important pollen source.
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45

Blanchetot, Alain. "DNA fingerprinting analysis in the solitary bee Megachile rotundata: variability and nest mate genetic relationships." Genome 35, no. 4 (August 1, 1992): 681–88. http://dx.doi.org/10.1139/g92-103.

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The most conventional approach for evaluating genetic variability in an insect population involves assessing the degree of enzyme polymorphism. Hymenoptera display a relatively low level of genetic variability compared with most insect species. DNA probes consisting of tandemly repeated sequences are powerful tools for detecting polymorphisms when employed to develop DNA fingerprinting (DNAfp) profiles in a wide range of organisms. This report describes genetic variability in the solitary bee species Megachile rotundata as assessed by DNAfp using the Ml3 sequence and a synthetic oligonucleotide sequence homologous to a hypervariable region of the α-globin gene. DNAfp comparisons among offspring were used to analyze genealogical structure in M. rotundata nests. The results indicate that polyandry, by a large number of males, is not a common phenomenon in M. rotundata bee species. In the present analysis, it is likely that the broods raised in single nests are mostly the offspring of one singly mated female. However, the data does not preclude that for certain nests two males could have been involved in the mating process.Key words: Megachile rotundata, DNA fingerprinting, M13 sequence, α-globin hypervariable sequence, intra-nest genetic relationships.
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Lu, Rui, and Gerald H. Rank. "Use of RAPD analyses to estimate population genetic parameters in the alfalfa leaf-cutting bee, Megachile rotundata." Genome 39, no. 4 (August 1, 1996): 655–63. http://dx.doi.org/10.1139/g96-083.

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RAPD analyses were performed on five geographically isolated populations of Megachile rotundata. We used haploid males of the alfalfa leaf-cutting bee, M. rotundata, to overcome the limitation of the dominance of RAPD markers in the determination of population genetic parameters. Sixteen primers gave rise to 130 polymorphic and 31 monomorphic bands. The unbiased estimators calculated in this study include within- and between-population heterozygosity, nucleotide divergence, and genetic distance. The genetic diversity (H = 0.32–0.35) was found to be about 10 times that of previous estimates (H = 0.033) based on allozyme data. Contrary to the data obtained at the protein level, our results suggest that Hymenoptera do not have a lower level of genetic variability at the DNA level compared with other insect species. Regardless of the different assumptions underlying the calculation of heterozygosity, divergence, and genetic distance, all five populations showed a parallel interrelationship for the three parameters. We conclude that RAPD markers are a convenient tool to estimate population genetic variation in haploid M. rotundata and that with an adequate sample size the technique is applicable to the evaluation of divergence in diploid populations. Key words : Megachile rotundata, RAPD, heterozygosity, genetic distance, nucleotide divergence.
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47

Falamarzi, Shahram, Behzad Habibpour, Mohammad S. Mossadegh, and Alireza Monfared. "Differentiation of Five Species of Megachile (Hymenoptera: Megachilidae), based on Wing Shape." Journal of Apicultural Science 60, no. 2 (December 1, 2016): 41–50. http://dx.doi.org/10.1515/jas-2016-0015.

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Abstract In the present work we used landmark-based geometric morphometrics to compare the wing shapes of five species of Megachile (belonging to three subgenera) to confirm whether this technique may be used reliably for differentiation of this group. Analyses of wing shape by the use of principal component analysis (PCA), and canonical variate analysis (CVA) led to a clear differentiation among species. We found a close phenotypic similarity in wing shape between M. albisecta (belonging to the subgenus Creightonella) and M. picicornis (belonging to the subgenus Eutricharaea). According to the results of UPGMA, a higher degree of divergence between M. farinosa (belonging to the subgenus Pseudomegachile) and species belonging to other subgenera, was detected. The results of a cross-validation test indicated that geometric morphometrics is an effective technique to use for distinguishing between Megachile species. The reliability rate of this technique was between 85.71-100%. Using only two submarginal cell landmarks for generating shape variables, the cross-validation test correctly assigned individuals to their respective species, with a 92.85-100% reliability rate. Significant differences in wing size were obtained among the analysed species.
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48

Mello, Bruno Nunes da Silva, and Maria Cristina Gaglianone. "Nesting Biology of Sympatric Species of Megachilidae Bees in a Conservation Area in Brazilian Atlantic Forest." Sociobiology 66, no. 1 (April 25, 2019): 52. http://dx.doi.org/10.13102/sociobiology.v66i1.2881.

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Megachilidae bees are important pollinators in the Neotropical region, however information on the ecology and behavior of these species is still scarce. The objective of this study was to analyze the nesting biology of sympatric species in the União Biological Reserve, a remnant of Atlantic Forest in the southeastern Brazil. Our results indicated the occurrence of 17 species, representing significant richness compared to other areas in the Atlantic Forest. Five sympatric species built ¾ of all nests found and the architecture of their nests was studied including, for the first time, nests of a species of Megachile (Ptilosarus). The use of petals or leaf fragments in the construction of the nests was observed for species of Megachile (Chrysosarus), confirming previous data. The nesting activity period in the trap-nests occurred mainly in the rainy season, with different peaks among the species. The results indicate that the distinctive characteristics of the species, such as the type of material used in the nests, the dimensions of cavities, and the asynchronous nesting period, could be important for the niche differentiation of these sympatric species, allowing the maintenance and the survival of the most abundant Megachilidae populations in the area.
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49

PRAZ, CHRISTOPHE, AHMED M. AL-SHAHAT, and NEVEEN S. GADALLAH. "Taxonomic revision of the subgenus Eutricharaea Thomson in Egypt, with a key to the species and the description of two new species (Hymenoptera, Anthophila, Megachilidae, genus Megachile Latreille)." Zootaxa 5032, no. 3 (September 8, 2021): 301–30. http://dx.doi.org/10.11646/zootaxa.5032.3.1.

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The subgenus Eutricharaea Thomson of the genus Megachile Latreille (Hymenoptera: Megachilidae) in Egypt is revised. Fifteen species are recognized, of which two are new: Megachile laniventris Praz sp. nov. and M. rufomandibularis Praz sp. nov. In addition, three unclear taxa known from few specimens in one sex are documented to facilitate future work. The following new synonymies are proposed: M. microxantha Cockerell 1937, M. privigna Rebmann 1968, and M. submucida Alfken 1926 are synonymized with M. leucostoma Pérez 1907 (syn. nov.); M. blanda Rebmann 1968 with M. walkeri Dalla Torre 1896 (syn. nov.); M. uniformis Alfken 1934 (not M. uniformis Mitchell 1929) and the replacement name M. minutuloides Alfken 1936 with M. minutissima Radoszkowski 1876 (syn. nov.); and M. tkalcui Zanden 1996 with M. rugipuncta Alfken 1934 (syn. nov.). Lectotypes are designated for M. soikai Benoist 1961, M. rugipuncta, M. tenuistriga Alfken 1938, M. niveascopa Ferton 1908, and M. naevia Kohl 1906. The previously unknown males of M. impressipuncta Alfken 1934 and M. rugipuncta, as well as the undescribed female of M. soikai are described. Illustrated keys for both males and females are provided and DNA barcodes are published for most species.
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50

Potapov, GS, and YuS Kolosova. "Contribution to the knowledge of the fauna of the long-tongued bees (Hymenoptera, Apoidea: Megachilidae, Apidae) in the north of the Arkhangelsk Region." Arctic Environmental Research 19, no. 3 (November 15, 2019): 99–105. http://dx.doi.org/10.3897/issn2541-8416.2019.19.3.99.

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This article is devoted to a study of the fauna of the long-tongued bees in the north of the Arkhangelsk Region, which is located in the northeast of the European part of Russia, but excludes the Arctic islands. This group includes the bees of the families Megachilidae and Apidae. Forty-four (44) species of bees were found in this region. Most of them (29) are bumblebees (genus Bombus). Fourteen (14) species are megachilid bees (genus Osmia, Coelioxys and Megachile) and one species is Apis mellifera. The largest number of species (39) was recorded in the lower reaches of the Northern Dvina River, due to the long research on this territory. The lowest number of species (16) was recorded in the Mezensky District. In the north of the studied region, the tundra species of B. lapponicus is only presented for the Mezensky District. Many species of bumblebees in the regional fauna belong to the ecological group of the meadow species. These are B. soroeensis, B. ruderarius, B. rupestris and a number of others. They are typical for meadow and ruderal habitats, and are usually not presented in the native taiga habitats. These meadow species are widely represented in the valleys of large rivers, such as the Northern Dvina, the Onega, and the Mezen. Compared to bumblebees, megachilid bees are much rarer in the north of the Arkhangelsk Region. Apis mellifera is presented in the lower reaches of the Northern Dvina River, but here it is rare, compared to the southern part of the Arkhangelsk Region.
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