Journal articles on the topic 'MEDIUM FOV'
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Nascimento, Helena Aguiar Ribeiro, Marcos Ely Almeida Andrade, Marco Antonio Gomes Frazão, Eduarda Helena Leandro Nascimento, Flavia Maria Moraes Ramos-Perez, and Deborah Queiroz Freitas. "Dosimetry in CBCT with Different Protocols: Emphasis on Small FOVs Including Exams for TMJ." Brazilian Dental Journal 28, no. 4 (August 2017): 511–16. http://dx.doi.org/10.1590/0103-6440201701525.
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Abstract This study aimed to estimate the absorbed dose in cone beam computed tomography (CBCT) exams according to different exposure parameters and size and position of the field of view (FOV). In addition was compared the absorbed dose of two smaller FOV scans with that of a larger FOV scan for evaluation of temporomandibular joint (TMJ), as it is a bilateral structure. CBCT scans were obtained on OP300 Maxio unit varying scanning mode (standard, high and endo) as well as size (5x5, 6x8 and 8x15 cm) and positioning of FOV. With a small FOV, different areas were scanned (maxilla or mandible, anterior or posterior and TMJ). Absorbed doses were determined using thermoluminescent dosimeters on the skin surface of sensitive organs of an anthropomorphic phantom. Endo mode showed the highest dose, followed by the high and standard modes in all FOV positions. With small FOV, doses were higher in the posterior region, especially in the mandible. Dose reduction occurred when small FOVs were used, but it was not proportional to FOV size reduction. For TMJ, the dose in a single acquisition with large FOV was greater than two acquisitions with small FOV, but lower than two acquisitions with medium FOV (6x8 cm). In conclusion, scanning mode, size and FOV position have great influence on the absorbed dose. Small FOV decreases the dose, but there is no linear relation between FOV size and dose. For bilateral exams of TMJ, double acquisition with small FOVs produces decrease in absorbed dose relative to a large FOV.
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Trindade, Hugo, Inês Morais, and Abigail Moreira. "EXTRAORAL AND CBCT DENTAL EXPOSURES IN PORTUGAL." Radiation Protection Dosimetry 190, no. 3 (July 2020): 283–88. http://dx.doi.org/10.1093/rpd/ncaa102.
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Abstract Data from dental extraoral and cone beam computed tomography (CBCT) exposures in Portugal (2019) were gathered, and patient doses for standard adult exams were evaluated. In panoramic X-rays, 442 units (34% of the existing licensed units) were tested, with a third quartile value (PKA) of 82 mGy.cm2. For cephalometric radiography (88 units), the third quartile value (Ki) was 0.3 mGy for the posteroanterior projection and 0.2 mGy for lateral projection. In CBCT (69 units), the doses for the placement of an upper first molar implant were evaluated with a third quartile value (PKA) of 820 mGy.cm2. Due to the wide range of values (74–3687 mGy.cm2), the CBCT data were divided by FOV dimensions: for small FOV (average FOV of 7 ×8 cm), a value of 580 mGy.cm2 was obtained and for medium FOV (average FOV of 13 × 12 cm) a value of 1167 mGy.cm2. The number of annual panoramic X-rays made in Portugal was 208 per 1000 inhabitants, which is higher than the value for other countries.
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Yu, Panpan, Yifan Liu, Yijing Wu, Jinghan Zhuang, Ziqiang Wang, Yinmei Li, and Lei Gong. "Large-FOV scattering-assisted holographic projection by enhanced sampling of transmission matrix." Applied Physics Letters 122, no. 6 (February 6, 2023): 061104. http://dx.doi.org/10.1063/5.0137279.
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Based on wavefront shaping, scattering materials provide a unique tool to break the trade-off between the viewing angle and field of view (FOV) for three-dimensional holographic projections. However, large-size image projection is limited by the low sampling ability of the transmission matrix (TM) of the scattering medium. Here, we propose a disperse and montage sampling strategy to access the TM for large-size image projection by scattering-assisted holography. Compared with the conventional TM sampling methods, our method achieves control of the output field with an enlarged FOV and improved adaptability. Experimentally, we achieve calibration of a TM corresponding to 1920 × 780 output pixels. As a proof of concept, we demonstrate holographic projection of large-size letter images within an area of 11.25 × 4.57 mm2 behind a scattering medium. In addition, we show that large-FOV vectorial projection can also be achieved by sampling the polarization-related TMs. Our work is expected to benefit scattering-assisted holographic techniques with potential applications in holographic display, imaging, and trapping.
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Elshenawy, Hanaa, Wessam Aly, Nashwa Salah, Sherine Nasry, Enas Anter, and Khalid Ekram. "The Influence of Small, Midi, Medium and Large Fields of View on Accuracy of Linear Measurements in CBCT Imaging: Diagnostic Accuracy Study." Open Access Macedonian Journal of Medical Sciences 7, no. 6 (March 29, 2019): 1037–41. http://dx.doi.org/10.3889/oamjms.2019.232.
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AIM: This study aimed to assess the effect of changing the field of view on the dimensional accuracy of CBCT imaging.
METHODS: The implant-bone models were randomly numbered from 1 to 13 by the principal researcher, and then on each model at the incisors region three positions were selected and marked on the model with a permanent blue marker. Then at each marked position three radio-opaque ‘RO’ markers “gutta-percha pieces” were glued on the model surfaces as following; two pieces on the facial surface one occlusally (at the alveolar crest) and one apically (at the inferior border of the model) both were on the same vertical line and perpendicular to the horizontal plane, while the third one was placed on the lingual surface opposing the occlusally placed buccal piece. CBCT examinations of each bone model were performed using Cranex3Dx CBCT (Helsinki, Finland) machine. Each model was scanned four times with standardised tube current and voltage of 12.5 mA and 90 kVp respectively at four different FOVs. The FOVs used were as following: Small FOV: 50 x 50 mm with voxel size 200 µm, Midi FOV: 61 x 78 mm with voxel size 300 µm, Medium FOV: 78 x 78 mm with voxel size 300 µm, Large FOV: 78 x 150 mm with voxel size 350 µm. The reference standard in this study was the real linear measurements that were obtained directly on the implant-bone models using high precision sliding electronic digital calliper with 0-150 mm internal and external measuring range and 0.01 mm resolution accuracy. The index test in the current study was the CBCT linear measurements obtained from CBCT images of implant-bone models using small, midi, medium and large FOVs.
RESULTS: The results of this study showed that both medium and large FOVs showed a statistically significant difference, which could be translated into clinical relevance only in thickness measurements.
CONCLUSION: The interpretation of these results leads to the assumption that increasing the FOV size together with voxel size could adversely affect the accuracy of CBCT linear measurements, especially when small distances are to be assessed.
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Tran, Hoang Quang Minh, and Anh Vu Le. "Fusing Detected Humans in Multiple Perception Sensors Network." Journal of Advanced Engineering and Computation 1, no. 2 (November 30, 2017): 114. http://dx.doi.org/10.25073/jaec.201712.61.
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A fusion method is proposed to keep a correct number of humans from all humans detected by the robot operating system based perception sensor network (PSN) which includes multiple partially overlapped field of view (FOV) Kinects. To this end, the fusion rules are based on the parallel and orthogonal configurations of Kinects in PSN system. For the parallel configuration, the system will decide whether the detected humans staying in FOV of single Kinect or in overlapped FOV of multiple Kinects by evaluating the angles formed between their locations and Kinect original point on top view (x, z plane) of 3D coordination. Then, basing on the angles, the PSN system will keep the person stay in only one FOV or keep the one with biggest ROI if they stay in overlapped FOV of Kinects. In the case of Kinects with orthogonal configuration, 3D Euclidian distances between detected humans are used to determine the group of humans supported to be same human but detected by different Kinects. Then the system, keep the human with a bigger region of interest (ROI) among this group. The experimental results demonstrate the outperforming of the proposed method in various scenarios. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Pantaleo, Francesca Romana. "The prototype Schwarzschild Couder Telescope: a Medium-Sized Telescope for the Cherenkov Telescope Array." Journal of Physics: Conference Series 2429, no. 1 (February 1, 2023): 012021. http://dx.doi.org/10.1088/1742-6596/2429/1/012021.
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Abstract The Schwarzschild Couder Telescope (SCT) is a dual mirror Medium-Sized telescope proposed for the Cherenkov Telescope Array (CTA), the next-generation very-high energy (from about 20 GeV to 300 TeV) gamma-rays observatory. The SCT design consists of a dual-mirror optics and a high-resolution camera with a field of view (FoV) of 8°, which will allow exceptional performance in terms of angular resolution and background rejection. A prototype telescope (pSCT) has been installed and is operating at the Fred Lawrence Whipple Observatory (FLWO) in Arizona, USA. Its camera is partially equipped with silicon photomultiplier (SiPM) matrices and covers a FoV of 2.7°. The pSCT has recently successfully detected the Crab Nebula with a statistical significance of 8.6 standard deviations. The upgrade of the pSCT focal plane is now ongoing, aimed to equip the full camera with upgraded sensors and electronics, enhancing the telescope field of view from the current 2.7° to the final 8°. An overview of the pSCT project and obtained results will be given, together with the camera upgrade status and expected performance.
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E., Suharyanto, Nurcahyani E., Hadisutrisno B, and Sumardi I. "ANATOMICAL CHARACTERIZATION OF SELECTED PLANLET Vanilla planifolia Andrews. ON FUSARIC ACID, RESISTANT TO Fusarium oxysporum f. sp. vanillae." KnE Life Sciences 2, no. 1 (September 20, 2015): 309. http://dx.doi.org/10.18502/kls.v2i1.164.
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<p>Vanilla is one of the important agricultural export commodity of Indonesia. The vanilla from Indonesia is well known for it high levels of vanillin content,which is about 2,75%. Until recently, the most hazardous disease of vanilla is foot rot infection caused by Fusarium oxysporum f. sp.vanillae (Fov). One alternative way to control the foot rot disease is by using cultivars which resistant to its pathogen. Selected plantlets have been induced by using fusaric acid on the in vitro medium. The goal of this research is to characterize the anatomical point of few especially on leaves, pseudo bulb and root with respect of resistant to Fov. Moreover, it is also addressed to determine the different characters of the phenotype vanilla plantlet whichare total of phenol, peroxydases enzyme activity, the thickness of lignin, and the chlorophyll content. The data on this research were recorded, compiled and categorized as descriptive qualitative and quantitative information. Theresult showed significant increase in chlorophyll a, chlorophyll b, and also the total of chlorophyll. The overall in line correlated with the increasing FA concentration. The total chlorophyll at 90 ppm FA could increased about 1,2 times, while at 100 ppm and 110 ppm FA could enhanced about 1,4 to 1,5 times compared to control. Anatomical characters between vanilla control, infected fov, and resistant to fov, were showed clearly at the stele part especially at xylem as the main function for transporting water from root to the leaf, which formed thyllose. Phenol total at about 4,39 % in control, increased to 5,34% in 90 ppm, and followed by 5,52 % in 100 ppm and 5,87 % at 110 ppm. Peroxidases enzyme activity at FA 90 ppm produced about 0,25 U/mg/min, and at 100 ppm FA on 0,34 U/mg/min, while at FA 110 ppm generated 0.36 U/mg/min compared to control, at 0,12 U/mg/min. The thickness of lignin tracheid in the treatment of 90 ppm was at about 14,59 ìm; 100 ppm 18,16 ìm; and 110 ppm 19,39 ìm compared to controls 10,28 ìm. In conclusion over all showed that FA induced plantlet vanilla inin vitro medium resulted in change of phenotype compare to control, which also affect its anatomy structure, especially on lignification and thyllose formation at surrounding tracheids, which could be use as marker for defend mechanism of plant to Fov.</p><p><br /><strong>Keywords</strong>: Fusarium oxysporum f. sp.Vanillae, peroxydases enzyme, anatomical characters.</p>
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Nurcahyani, E., E. Suharyanto, B. Hadisutrisno, and I. Sumardi. "IN VITRO SELECTION ON FUSARIC ACID OF Vanilla planifolia PLANTLETS FOR OBTAINING A CULTIVAR, WHICH RESISTANT TO Fusarium oxysporum f. sp. vanillae." KnE Life Sciences 2, no. 1 (September 20, 2015): 617. http://dx.doi.org/10.18502/kls.v2i1.229.
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<p>The most production constrain on Vanilla planifolia Andrews plantation recently has been caused by foot rot disease that later influence in decreasing the yield product. This disease is caused by Fusarium oxysporum f. sp. vanillae (Fov). So far, the disease has not been successfully prohibited although some experiments had been conducted. The use of foot rot resistant cultivar has been introduced, which expected has high yield as one alternative method for controlling this disease. A resistant vanilla plantlet to Fov has been initiated by in vitro selection on MS medium containing fusaric acid (FA) on selective concentration. The purpose of research were to study and determine: 1) The proper combination of 2,4-D and NAA for callus initiation and shoot development from shoot tip explants, along with proper concentration of BAP for shoots initiation from nodal explants; 2) The FA concentration of plantlet selection tolerant to steady growth; 3) The proper concentration of FA for in vitro selection for suppressing the Fov. Results showed that: 1) the proper concentration of both combination between 2,4-D and NAA is about 2,0 mg/L and 10 mg/L respectively for callus initiation and shoot development from shoot tip explants; also the BA concentration is 1,0 mg/L for shoot initiation from a nodal explants; 2) the FA tolerant concentration for plantlet selection with vanilla steady growth is between 90 ppm-110 ppm; 3) the 110 ppm of FA was effective for suppressing the Fov compared to 90 ppm and 100 ppm respectively. </p><p><strong>Keywords</strong>: Vanilla planifolia Andrews, the vanilla foot rot disease, Fusarium oxysporum f.sp. vanillae, in vitro, fusaric acid</p>
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Mamyrbayev, Talgat, Katsumasa Ikematsu, Hidekazu Takano, Yanlin Wu, Kenji Kimura, Patrick Doll, Arndt Last, Atsushi Momose, and Pascal Meyer. "Staircase array of inclined refractive multi-lenses for large field of view pixel super-resolution scanning transmission hard X-ray microscopy." Journal of Synchrotron Radiation 28, no. 3 (March 12, 2021): 732–40. http://dx.doi.org/10.1107/s1600577521001521.
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Owing to the development of X-ray focusing optics during the past decades, synchrotron-based X-ray microscopy techniques allow the study of specimens with unprecedented spatial resolution, down to 10 nm, using soft and medium X-ray photon energies, though at the expense of the field of view (FOV). One of the approaches to increase the FOV to square millimetres is raster-scanning of the specimen using a single nanoprobe; however, this results in a long data acquisition time. This work employs an array of inclined biconcave parabolic refractive multi-lenses (RMLs), fabricated by deep X-ray lithography and electroplating to generate a large number of long X-ray foci. Since the FOV is limited by the pattern height if a single RML is used by impinging X-rays parallel to the substrate, many RMLs at regular intervals in the orthogonal direction were fabricated by tilted exposure. By inclining the substrate correspondingly to the tilted exposure, 378000 X-ray line foci were generated with a length in the centimetre range and constant intervals in the sub-micrometre range. The capability of this new X-ray focusing device was first confirmed using ray-tracing simulations and then using synchrotron radiation at BL20B2 of SPring-8, Japan. Taking account of the fact that the refractive lens is effective for focusing high-energy X-rays, the experiment was performed with 35 keV X-rays. Next, by scanning a specimen through the line foci, this device was used to perform large FOV pixel super-resolution scanning transmission hard X-ray microscopy (PSR-STHXM) with a 780 ± 40 nm spatial resolution within an FOV of 1.64 cm × 1.64 cm (limited by the detector area) and a total scanning time of 4 min. Biomedical implant abutments fabricated via selective laser melting using Ti–6Al–4V medical alloy were measured by PSR-STHXM, suggesting its unique potential for studying extended and thick specimens. Although the super-resolution function was realized in one dimension in this study, it can be expanded to two dimensions by aligning a pair of presented devices orthogonally.
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Melo, Thiago Anchieta de, Ilka Márcia Ribeiro de Souza Serra, and Ingrid Tayane Vieira da Silva do Nascimento. "Ascophyllum nodosum seaweed extract effect on morphology and cellulolytic ability of the fungus Fusarium oxysporum f. sp. vasinfectum." Research, Society and Development 9, no. 11 (November 19, 2020): e4079119913. http://dx.doi.org/10.33448/rsd-v9i11.9913.
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This work aimed to verify the effect in vitro, of Ascophyllum nodosum (AN) seaweed extract on the morphology and cellulolytic capacity of the fungus Fusarium oxysporum f. sp. vasinfectum (FOV). Thus, the fungus was placed in contact with different doses of the extract, being these: 0, 0.5, 1.0, 2.0, 4.0 and 8.0%. It was verified that the product, with increasing doses, progressively induced mycelial growth of the fungus, as measured by the diameter of the colonies and fresh mass of mycelium grown in PD (potato-dextrose) culture medium. This result was also corroborated by the progressive increase in the activity of the β-1,3-glucanase and chitinase enzymes required during the hypha elongation process. However, the AN extract progressively reduced FOV sporulation with increasing doses. Furthermore, the cellulolytic capacity of the phytopathogen was significantly reduced in the presence of the algae extract, which was measured by the activity of the enzymes endo-β-1,4-glucanase, exo-β-1,4-glucanase and β-glucosidase. Thus, these facts constitute important information for the management of fusariosis, since the inhibition of sporulation and decreasing degradation capacity of the cellulose by the pathogen, can translate into declined disease in compatible host-pathogen interactions.
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Shi, YingJie, EnLai Guo, Ming Sun, LianFa Bai, and Jing Han. "Non-invasive imaging through scattering medium and around corners beyond 3D memory effect." Optics Letters 47, no. 17 (August 22, 2022): 4363. http://dx.doi.org/10.1364/ol.470222.
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The three-dimensional (3D) memory effect (ME) has been shown to exist in a variety of scattering scenes. Limited by the scope of ME, speckle correlation technology only can be applied in a small imaging field of view (FOV) with a small depth of field (DOF). In this Letter, an untrained neural network is constructed and used as an optimization tool to restore the targets beyond the 3D ME range. The autocorrelation consistency relationship and the generative adversarial strategy are combined. Only single frame speckle and unaligned real targets are needed for online optimization; therefore, the neural network does not need to train in advance. Furthermore, the proposed method does not need to conduct additional modulation for the system. This method can reconstruct not only hidden targets behind the scattering medium, but also targets around corners. The combination strategy of the generative adversarial framework with physical priors used to decouple the aliasing information and reconstruct the target will provide inspiration for the field of computational imaging.
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Park, George D., R. Wade Allen, Theodore J. Rosenthal, and Dary Fiorentino. "Training Effectiveness: How Does Driving Simulator Fidelity Influence Driver Performance?" Proceedings of the Human Factors and Ergonomics Society Annual Meeting 49, no. 25 (September 2005): 2201–5. http://dx.doi.org/10.1177/154193120504902518.
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Driver performance effects were compared between two configuration types: 1) a low-cost, three-monitor, 135 degree field-of-view (FOV), PC desktop with PC gaming steering wheel controls and 2) a medium-cost, fixed-based, projected image, 135 degree FOV, instrumented vehicle cab. The experiment was part of a larger novice driver training experiment with teenage drivers who had yet to receive their license to drive (Allen, Park, et al. 2003). Participants drove a minimum of six training trial runs on either the three-monitor configuration (N = 180) or the vehicle cab configuration (N = 143). A 2 times 6 (configuration type x training trial runs) analysis of variance was performed for a variety of performance measures as well as a one-way analysis of variance to assess the graduation rates between the two configurations. Significant differences were found for certain performance measures suggesting that handling behaviors (i.e. braking and steering) were largely affected by the difference in controls while lane position, vehicle speed, time-to-collision, and simulator sickness ratings were largely affected by the difference in graphical display. However, non-significant differences in certain performance measures (e.g. total accidents and graduation rates) suggested that the three-monitor configuration may be as useful of a tool for driver training, assessment, and research as a higher fidelity vehicle cab.
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Zhang, Chunming, Chengli Qi, Tianhang Yang, Mingjian Gu, Panxiang Zhang, Lu Lee, Mengzhen Xie, and Xiuqing Hu. "Evaluation of FY-3E/HIRAS-II Radiometric Calibration Accuracy Based on OMB Analysis." Remote Sensing 14, no. 13 (July 4, 2022): 3222. http://dx.doi.org/10.3390/rs14133222.
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Before infrared hyperspectral data are used in satellite data assimilation systems or retrieval systems, the quantitative analysis of data deviation is necessary. Based on RTTOV’s (Radiative Transfer for TOVS) simulation data of FY-3E/HIRAS-II (Hyperspectral Infrared Atmospheric Sounder) and the observation data of HIRAS-II, we counted the bias of observation minus simulation (OMB) during an on-orbit test; analyzed the characteristics and reasons for the bias from the perspective of the FOV (field of view), the scanning angle of the instrument, the day and night, and the target temperature change; and analyzed the stability of the radiometric calibration accuracy. We also combined the results of the MetOp-C/IASI (infrared atmospheric sounding interferometer), a similar high-precision instrument, with the bias of OMB to compare and evaluate the FY-3E/HIRAS-II radiometric calibration accuracy. In the end, we found that the mean OMB bias of the long-wave and medium-wave infrared bands is within ±2 K, and the bias standard deviation is better than 2 K; the bias of each FOV is consistent and the bias of most channels is better than 2 K. The OMB bias of each channel is consistent with the changes in the angle of the instrument. The bias trend of long-wave and medium-wave infrared channels is more consistent with the deviation of the day and night; the bias of the short-wave infrared channel at night is lower than in the daytime. When counting the bias as the target temperature changed, the results showed that there are no obvious temperature dependencies in the long-wave and medium-wave infrared channels. This reflects that the instrument’s non-linear effect is well ordered. We further evaluated the stability of the radiometric calibration accuracy through statistics from the OMB standard deviation of each channel of FY-3E/HIRAS-II. Most channel accuracy stability values were better than 0.1 K. We calculated that IASI and HIRAS-II OMB have double differences, and the results show that the double difference in most channels is better than 1 K. It shows that the HIRAS-II and IASI observations are highly consistent. Through the statistics of the OMB bias during the on-orbit test period of FY-3E/HIRAS-II, we fully evaluated its radiometric calibration accuracy and laid the foundation for FY-3E/HIRAS-II data to be used in the retrieval application and assimilation system.
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Blanc, Guillermo A. "The Mitchell Spectrograph: Studying Nearby Galaxies with the VIRUS Prototype." Advances in Astronomy 2013 (2013): 1–16. http://dx.doi.org/10.1155/2013/641612.
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The Mitchell Spectrograph (a.k.a. VIRUS-P) on the 2.7 m Harlan J. Smith telescope at McDonald Observatory is currently the largest field of view (FOV) integral field unit (IFU) spectrograph in the world (1.7′×1.7′). It was designed as a prototype for the highly replicable VIRUS spectrograph which consists of a mosaic of IFUs spread over a16′diameter FOV feeding 150 spectrographs similar to the Mitchell. VIRUS will be deployed on the 9.2 meter Hobby-Eberly Telescope (HET) and will be used to conduct the HET Dark Energy Experiment (HETDEX). Since seeing first light in 2007 the Mitchell Spectrograph has been widely used, among other things, to study nearby galaxies in the local universe where their internal structure and the spatial distribution of different physical parameters can be studied in great detail. These observations have provided important insight into many aspects of the physics behind the formation and evolution of galaxies and have boosted the scientific impact of the 2.7 meter telescope enormously. Here I review the contributions of the Mitchell Spectrograph to the study of nearby galaxies, from the investigation the spatial distribution of dark matter and the properties of supermassive black holes, to the studies of the process of star formation and the chemical composition of stars and gas in the ISM, which provide important information regarding the formation and evolution of these systems. I highlight the fact that wide field integral field spectrographs on small and medium size telescopes can be powerful cost effective tools to study the astrophysics of galaxies. Finally I briefly discuss the potential of HETDEX for conducting studies on nearby galaxies. The survey parameters make it complimentary and competitive to ongoing and future surveys like SAMI and MANGA.
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Chen, Jing, Xingjie Lan, Ruimin Jia, Lifang Hu, and Yang Wang. "Response Surface Methodology (RSM) Mediated Optimization of Medium Components for Mycelial Growth and Metabolites Production of Streptomyces alfalfae XN-04." Microorganisms 10, no. 9 (September 16, 2022): 1854. http://dx.doi.org/10.3390/microorganisms10091854.
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Streptomyces alfalfae XN-04 has been reported for the production of antifungal metabolites effectively to control Fusarium wilt of cotton, caused by Fusarium oxysporum f. sp. vasinfectum (Fov). In this study, we used integrated statistical experimental design methods to investigate the optimized liquid fermentation medium components of XN-04, which can significantly increase the antifungal activity and biomass of XN-04. Seven variables, including soluble starch, KNO3, soybean cake powder, K2HPO4, MgSO4·7H2O, CaCO3 and FeSO4·7H2O, were identified as the best ingredients based on one-factor-at-a-time (OFAT) method. The results of Plackett–Burman Design (PBD) showed that soluble starch, soybean cake powder and K2HPO4 were the most significant variables among the seven variables. The steepest climbing experiment and response surface methodology (RSM) were performed to determine the interactions among these three variables and fine-tune the concentrations. The optimal compositions of medium were as follows: soluble starch (26.26 g/L), KNO3 (1.00 g/L), soybean cake powder (23.54 g/L), K2HPO4 (0.27 g/L), MgSO4·7H2O (0.50 g/L), CaCO3 (1.00 g/L) and FeSO4·7H2O (0.10 g/L). A verification experiment was then carried out under the optimized conditions, and the results revealed the mycelial dry weight of S. alfalfae XN-04 reaching 6.61 g/L. Compared with the initial medium, a 7.47-fold increase in the biomass was achieved using the optimized medium. Moreover, the active ingredient was purified from the methanol extract of S. alfalfae XN-04 mycelium and then identified as roflamycoin (a polyene macrolide antibiotic). The results may provide new insights into the development of S. alfalfae XN-04 fermentation process and the control of the Fusarium wilt of cotton and other plant diseases.
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Qin, Yimin, Eric Bloomquist, Tanyel Bulbul, and Joseph Gabbard. "Measuring the impacts of AR HMD on users’ situation awareness during wood frame assembly tasks." Journal of Information Technology in Construction 28 (February 28, 2023): 70–86. http://dx.doi.org/10.36680/j.itcon.2023.004.
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Advances in the development of Augmented Reality (AR) hardware and software allow for novel opportunities to positively influence the traditional construction industry. Recent research studied the feasibility of various AR devices for supporting construction assembly tasks, however, there is limited work examining the usability of AR head-mounted display (HMD) in relation to construction workers’ cognitive skills such as situation awareness. This study evaluated three information display types (paper blueprint, tag-along image and conformal model) during wood frame assembly of three different scales (small, medium, and large) to investigate how AR HMDs impact user’s situation awareness with respect to mental workload. Eighteen construction engineering students were recruited in a within-subjects experiment. The findings showed no significant difference in both the mental workload and the situation awareness for all three display types, suggesting that AR displays did not generate significant excessive mental burden or distractions on users in comparison to traditional paper-based information. Specifically comparing the AR displays, the results revealed that user’s mental workload was affected by field of view (FOV) restriction in AR HMD, whereas situation awareness wasn’t impacted.
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Ortega-Macareno, Luis C., and Lourdes G. Iglesias-Andreu. "Stimulating Effect of Salicylic Acid in the In Vitro and In Vivo Culture of Vanilla (Vanilla planifolia Jacks.)." AGRIVITA Journal of Agricultural Science 44, no. 1 (February 1, 2022): 48–54. http://dx.doi.org/10.17503/agrivita.v44i1.3003.
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Vanilla planifolia Jacks., is the only commercially cultivated orchid for its compound vanillin. This crop has had serious production limitations due to its susceptibility to Fusarium oxysporum f. sp. vanilla (Fov). Salicylic acid (SA) regulates growth and development processes and induces resistance in plants. Our study evaluated its effect on the growth and development of V. planifolia seedlings grown in vitro and in vivo. A completely randomized design was used with seedlings obtained from nodal segments (2-3 cm long) grown in MS medium with different concentrations of SA (0, 0.25, 5, and 7.5 μmol). An experimental design with four SA concentration treatments (0, 0.25, 0.5, and 1 mmol) and 12 replications was used to evaluate the effects of SA on greenhousegrown plantlets. During four weeks, the number of shoots, length, thickness, number of leaves, number and length roots, chlorophyll and carotene content for both conditions were observed. We found the 7.5 μmol and 1 mmol SA stimulated the growth and development of vanilla plantlets grown both in vivo and in vitro. SA does not affect the content of photosynthetic pigments under any growth conditions.
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Poulose, Alwin. "Simulation of an Indoor Visible Light Communication System Using Optisystem." Signals 3, no. 4 (November 1, 2022): 765–93. http://dx.doi.org/10.3390/signals3040046.
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Visible light communication (VLC ) is an emerging research area in wireless communication. The system works the same way as optical fiber-based communication systems. However, the VLC system uses free space as its transmission medium. The invention of the light-emitting diode (LED) significantly updated the technologies used in modern communication systems. In VLC, the LED acts as a transmitter and sends data in the form of light when the receiver is in the line of sight (LOS) condition. The VLC system sends data by blinking the light at high speed, which is challenging to identify by human eyes. The detector receives the flashlight at high speed and decodes the transmitted data. One significant advantage of the VLC system over other communication systems is that it is easy to implement using an LED and a photodiode or phototransistor. The system is economical, compact, inexpensive, small, low power, prevents radio interference, and eliminates the need for broadcast rights and buried cables. In this paper, we investigate the performance of an indoor VLC system using Optisystem simulation software. We simulated an indoor VLC system using LOS and non-line-of-sight (NLOS) propagation models. Our simulation analyzes the LOS propagation model by considering the direct path with a single LED as a transmitter. The NLOS propagation model-based VLC system analyses two scenarios by considering single and dual LEDs as its transmitter. The effect of incident and irradiance angles in an LOS propagation model and an eye diagram of LOS/NLOS models are investigated to identify the signal distortion. We also analyzed the impact of the field of view (FOV) of an NLOS propagation model using a single LED as a transmitter and estimated the bitrate (Rb). Our theoretical results show that the system simulated in this paper achieved bitrates in the range of 2.1208×107 to 4.2147×107 bits/s when the FOV changes from 30∘ to 90∘. A VLC hardware design is further considered for real-time implementations. Our VLC hardware system achieved an average of 70% data recovery rate in the LOS propagation model and a 40% data recovery rate in the NLOS propagation model. This paper’s analysis shows that our simulated VLC results are technically beneficial in real-world VLC systems.
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Brunamonti, Simone, Giovanni Martucci, Gonzague Romanens, Yann Poltera, Frank G. Wienhold, Maxime Hervo, Alexander Haefele, and Francisco Navas-Guzmán. "Validation of aerosol backscatter profiles from Raman lidar and ceilometer using balloon-borne measurements." Atmospheric Chemistry and Physics 21, no. 3 (February 16, 2021): 2267–85. http://dx.doi.org/10.5194/acp-21-2267-2021.
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Abstract. Remote-sensing measurements by light detection and ranging (lidar) instruments are fundamental for the monitoring of altitude-resolved aerosol optical properties. Here we validate vertical profiles of aerosol backscatter coefficient (βaer) measured by two independent lidar systems using co-located balloon-borne measurements performed by Compact Optical Backscatter Aerosol Detector (COBALD) sondes. COBALD provides high-precision in situ measurements of βaer at two wavelengths (455 and 940 nm). The two analyzed lidar systems are the research Raman Lidar for Meteorological Observations (RALMO) and the commercial CHM15K ceilometer (Lufft, Germany). We consider in total 17 RALMO and 31 CHM15K profiles, co-located with simultaneous COBALD soundings performed throughout the years 2014–2019 at the MeteoSwiss observatory of Payerne (Switzerland). The RALMO (355 nm) and CHM15K (1064 nm) measurements are converted to 455 and 940 nm, respectively, using the Ångström exponent profiles retrieved from COBALD data. To account for the different receiver field-of-view (FOV) angles between the two lidars (0.01–0.02∘) and COBALD (6∘), we derive a custom-made correction using Mie-theory scattering simulations. Our analysis shows that both lidar instruments achieve on average a good agreement with COBALD measurements in the boundary layer and free troposphere, up to 6 km altitude. For medium-high-aerosol-content measurements at altitudes below 3 km, the mean ± standard deviation difference in βaer calculated from all considered soundings is −2 % ± 37 % (−0.018 ± 0.237 Mm−1 sr−1 at 455 nm) for RALMO−COBALD and +5 % ± 43 % (+0.009 ± 0.185 Mm−1 sr−1 at 940 mm) for CHM15K−COBALD. Above 3 km altitude, absolute deviations generally decrease, while relative deviations increase due to the prevalence of air masses with low aerosol content. Uncertainties related to the FOV correction and spatial- and temporal-variability effects (associated with the balloon's drift with altitude and different integration times) contribute to the large standard deviations observed at low altitudes. The lack of information on the aerosol size distribution and the high atmospheric variability prevent an accurate quantification of these effects. Nevertheless, the excellent agreement observed in individual profiles, including fine and complex structures in the βaer vertical distribution, shows that under optimal conditions, the discrepancies with the in situ measurements are typically comparable to the estimated statistical uncertainties in the remote-sensing measurements. Therefore, we conclude that βaer profiles measured by the RALMO and CHM15K lidar systems are in good agreement with in situ measurements by COBALD sondes up to 6 km altitude.
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Ludlow, John B., and Marija Ivanovic. "Weightbearing CBCT, MDCT, and 2D imaging dosimetry of the foot and ankle." International Journal of Diagnostic Imaging 1, no. 2 (March 5, 2014): 1. http://dx.doi.org/10.5430/ijdi.v1n2p1.
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Aims: This study compares effective doses associated with 2D lateral, oblique, and AP radiographs with 3D MDCT and Cone Beam CT images of the foot and ankle. Methods: An anthropomorphic phantom of the foot and ankle was constructed from an adult human skeleton and soft tissue equivalent material. Optical stimulated dosimeters were placed at 21 locations within and on tissues and anatomy of interest. Effective dose was calculated following 2007 ICRP recommendations. Three projections were exposed to simulate conventional 2D imaging. Standard and optimized dose MDCT scans were exposed to simulate typical CT options. Ten, 20, and 30cm fields of view, 100 and 120kVp and 4.5 and 6.8mAs exposures were tested using a PedCAT® CBCT device designed for weightbearing imaging. Dose was calculated for an adult as well as for a 5 and 10-year-old child to assess the impact of age on risk estimation. Results: Standard adult effective doses for single foot imaging were 0.6µSv for 2D, 3.8µSv for CBCT, and 25µSv for MDCT (p = 0.0013). Dose differed significantly with age (p = 0.0185). For a 5-year-old, doses rise to 0.8µSv for 2D, 18µSv for CBCT, and 200µSv for MDCT. Small and medium CBCT fields produced adult doses of 2.3 mSv and 0.9 mSv respectively. Conclusions: The effective dose for small FOV CBCT or conventional 2D series examinations is comparable to a few hours of equivalent background dose. Such doses are negligible; therefore, the dose of radiation should not be a concern when considering the use of CBCT imaging for foot/ankle examination.
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Della Bruna, Lorenza, Angela Adamo, Janice C. Lee, Linda J. Smith, Mark Krumholz, Arjan Bik, Daniela Calzetti, et al. "Studying the ISM at ∼10 pc scale in NGC 7793 with MUSE." Astronomy & Astrophysics 650 (June 2021): A103. http://dx.doi.org/10.1051/0004-6361/202039402.
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Context. Feedback from massive stars affects the interstellar medium (ISM) from the immediate surroundings of the stars (parsec scales) to galactic (kiloparsec) scales. High-spatial resolution studies of H II regions are critical to investigate how this mechanism operates. Aims. We study the ionised ISM in NGC 7793 with the MUSE instrument at ESO Very Large Telescope (VLT), over a field of view (FoV) of ∼2 kpc2 and at a spatial resolution of ∼10 pc. The aim is to link the physical conditions of the ionised gas (reddening, ionisation status, abundance measurements) within the spatially resolved H II regions to the properties of the stellar populations producing Lyman continuum photons. Methods. The analysis of the MUSE dataset, which provides a map of the ionised gas and a census of Wolf Rayet stars, is complemented with a sample of young star clusters (YSCs) and O star candidates observed with the Hubble Space Telescope (HST) and of giant molecular clouds traced in CO(2–1) emission with the Atacama Large Millimeter/submillimeter Array (ALMA). We estimated the oxygen abundance using a temperature-independent strong-line method. We determined the observed total amount of ionising photons (Q(H0)) from the extinction corrected Hα luminosity. This estimate was then compared to the expected Q(H0) obtained by summing the contributions of YSCs and massive stars. The ratio of the two values gives an estimate for the escape fraction (fesc) of photons in the region of interest. We used the [S II]/[O III] ratio as a proxy for the optical depth of the gas and classified H II regions into ionisation bounded, or as featuring channels of optically thin gas. We compared the resulting ionisation structure with the computed fesc. We also investigated the dependence of fesc on the age spanned by the stellar population in each region. Results. We find a median oxygen abundance of 12 + log(O/H) ∼ 8.37, with a scatter of 0.25 dex, which is in agreement with previous estimates for our target. We furthermore observe that the abundance map of H II regions is rich in substructures, surrounding clusters and massive stars, although clear degeneracies with photoionisation are also observed. From the population synthesis analysis, we find that YSCs located in H II regions have a higher probability of being younger and less massive as well as of emitting a higher number of ionising photons than clusters in the rest of the field. Overall, we find fesc,HII = 0.67−0.12+0.08 for the population of H II regions. We also conclude that the sources of ionisation observed within the FoV are more than sufficient to explain the amount of diffuse ionised gas (DIG) observed in this region of the galaxy. We do not observe a systematic trend between the visual appearance of H II regions and fesc, pointing to the effect of 3D geometry in the small sample probed.
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Ibrahim, M. Syed, Wahab Uddin, Bhuwan Joshi, Ramesh Chandra, and Arun Kumar Awasthi. "Investigation of two coronal mass ejections from circular ribbon source region:Origin, Sun-Earth propagation and Geoeffectiveness." Research in Astronomy and Astrophysics 21, no. 12 (February 1, 2022): 318. http://dx.doi.org/10.1088/1674-4527/21/12/318.
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Abstract In this article, we compare the properties of two coronal mass ejections (CMEs) that show similar source region characteristics but different evolutionary behaviors in the later phases. We discuss the two events in terms of their near-Sun characteristics, interplanetary evolution and geoeffectiveness. We carefully analyzed the initiation and propagation parameters of these events to establish the precise CME-interplanetary CME (ICME) connection and their near-Earth consequences. The first event is associated with poor geomagnetic storm disturbance index (Dst ≈-20 nT) while the second event is associated with an intense geomagnetic storm of DST ≈-119 nT. The configuration of the sunspots in the active regions and their evolution are observed by Helioseismic and Magnetic Imager (HMI). For source region imaging, we rely on data obtained from Atmospheric Imaging Assembly (AIA) on board Solar Dynamics Observatory (SDO) and Hα filtergrams from the Solar Tower Telescope at Aryabhatta Research Institute of Observational Sciences (ARIES). For both the CMEs, flux rope eruptions from the source region triggered flares of similar intensities (≈M1). At the solar source region of the eruptions,we observed a circular ribbon flare (CRF) for both cases, suggesting fan-spine magnetic configuration in the active region corona. The multi-channel SDO observations confirm that the eruptive flares and subsequent CMEs were intimately related to the filament eruption. Within the Large Angle and Spectrometric Coronograph (LASCO) field of view (FOV) thetwo CMEs propagated with linear speeds of 671 and 631 km s−1, respectively. These CMEs were tracked up to the Earth by Solar Terrestrial Relations Observatory (STEREO) instruments. We find that the source region evolution of CMEs, guided by the large-scale coronal magnetic field configuration, along with near-Sun propagation characteristics, such as CME-CME interactions, played important roles in deciding the evolution of CMEs in the interplanetary medium and subsequently their geoeffectiveness.
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Cao, Yunfeng, Manyao Li, and Yuzhen Zhang. "Estimating the Clear-Sky Longwave Downward Radiation in the Arctic from FengYun-3D MERSI-2 Data." Remote Sensing 14, no. 3 (January 27, 2022): 606. http://dx.doi.org/10.3390/rs14030606.
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Surface longwave downward radiation (LWDR) plays a key role in determining the Arctic surface energy budget, especially in insolation-absent boreal winter. A reliable LWDR product is essential for understanding the intrinsic physical mechanisms of the rapid changes in the Arctic climate. The Medium-Resolution Spectral Imager (MERSI-2), a major payload of the Chinese second-generation polar-orbiting meteorological satellite, FengYun-3D (FY-3D), was designed similar to the NASA Moderate-Resolution Imaging Spectroradiometer (MODIS) in terms of the spectral bands. Although significant progress has been made in estimating clear-sky LWDR from MODIS observations using a variety of methods, few studies have focused on the retrieval of clear-sky LWDR from FY-3D MERSI-2 observations. In this study, we propose an advanced method to directly estimate the clear-sky LWDR in the Arctic from the FY-3D MERSI-2 thermal infrared (TIR) top-of-atmosphere (TOA) radiances and auxiliary information using the extremely randomized trees (ERT) machine learning algorithm. The retrieval accuracy of RMSE and bias, validated with the Baseline Surface Radiation Network (BSRN) in situ measurements, are 14.14 W/m2 and 4.36 W/m2, respectively, which is comparable and even better than previous studies. The scale effect in retrieval accuracy evaluation was further analyzed and showed that the validating window size could significantly influence the retrieval accuracy of the MERSI-2 clear-sky LWDR dataset. After aggregating to a spatial resolution of 9 km, the RMSE and bias of MERSI-2 retrievals can be reduced to 9.43 W/m2 and −0.14 W/m2, respectively. The retrieval accuracy of MERSI-2 clear-sky LWDR at the CERES SSF FOV spatial scale (approximately 20 km) can be further reduced to 8.64 W/m2, which is much higher than the reported accuracy of the CERES SSF products. This study demonstrates the feasibility of producing LWDR datasets from Chinese FY-3D MERSI-2 observations using machine learning methods.
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Palaniappan, Prasannakumar, Sebastian Meyer, Martin Rädler, Florian Kamp, Claus Belka, Marco Riboldi, Katia Parodi, and Chiara Gianoli. "X-ray CT adaptation based on a 2D–3D deformable image registration framework using simulated in-room proton radiographies." Physics in Medicine & Biology 67, no. 4 (February 9, 2022): 045003. http://dx.doi.org/10.1088/1361-6560/ac4ed9.
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Abstract The aim of this work is to investigate in-room proton radiographies to compensate realistic rigid and non-rigid transformations in clinical-like scenarios based on 2D–3D deformable image registration (DIR) framework towards future clinical implementation of adaptive radiation therapy (ART). Monte Carlo simulations of proton radiographies (pRads) based on clinical x-ray CT of a head and neck, and a brain tumor patients are simulated for two different detector configurations (i.e. integration-mode and list-mode detectors) including high and low proton statistics. A realistic deformation, derived from cone beam CT of the patient, is applied to the treatment planning CT. Rigid inaccuracies in patient positioning are also applied and the effect of small, medium and large fields of view (FOVs) is investigated. A stopping criterion, as desirable in realistic scenarios devoid of ground truth proton CT (pCT), is proposed and investigated. Results show that rigid and non-rigid transformations can be compensated based on a limited number of low dose pRads. The root mean square error with respect to the pCT shows that the 2D–3D DIR of the treatment planning CT based on 10 pRads from integration-mode data and 2 pRads from list-mode data is capable of achieving comparable accuracy (∼90% and >90%, respectively) to conventional 3D–3D DIR. The dice similarity coefficient over the segmented regions of interest also verifies the improvement in accuracy prior to and after 2D–3D DIR. No relevant changes in accuracy are found between high and low proton statistics except for 2 pRads from integration-mode data. The impact of FOV size is negligible. The convergence of the metric adopted for the stopping criterion indicates the optimal convergence of the 2D–3D DIR. This work represents a further step towards the potential implementation of ART in proton therapy. Further computational optimization is however required to enable extensive clinical validation.
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Yang, Ke, Xiao Yang, Chaoru Gao, Changyi Hua, Chenggang Hong, and Ling Zhu. "A Novel Microfluidic Device for the Neutrophil Functional Phenotype Analysis: Effects of Glucose and Its Derivatives AGEs." Micromachines 12, no. 8 (August 11, 2021): 944. http://dx.doi.org/10.3390/mi12080944.
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Neutrophil dysfunction is closely related to the pathophysiology of patients with diabetes mellitus, but existing immunoassays are difficult to implement in clinical applications, and neutrophil’s chemotaxis as a functional biomarker for diabetes mellitus prognostic remains largely unexplored. Herein, a novel microfluidic device consisted of four independent test units with four cell docking structures was developed to study the neutrophil chemotaxis, which allowed multiple cell migration observations under a single field of view (FOV) and guaranteed more reliable results. In vitro studies, the chemotaxis of healthy neutrophils to N-Formyl-Met-Leu-Phe (fMLP) gradient (0, 10, 100, and 1000 nM) was concentration-dependent. The distinct promotion or suppression in the chemotaxis of metformin or pravastatin pretreated cells were observed after exposure to 100 nM fMLP gradient, indicating the feasibility and efficiency of this novel microfluidic device for clinically relevant evaluation of neutrophil functional phenotype. Further, the chemotaxis of neutrophils pretreated with 25, 50, or 70 mM of glucose was quantitatively lower than that of the control groups (i.e., 5 mM normal serum level). Neutrophils exposed to highly concentrated advanced glycation end products (AGEs) (0.2, 0.5, or 1.0 μM; 0.13 μM normal serum AGEs level), a product of prolonged hyperglycemia, showed that the higher the AGEs concentration was, the weaker the migration speed became. Specifically, neutrophils exposed to high concentrations of glucose or AGEs also showed a stronger drifting along with the flow, further demonstrating the change of neutrophil chemotaxis. Interestingly, adding the N-benzyl-4-chloro-N-cyclohexylbenzamide (FPS-ZM1) (i.e., high-affinity RAGE inhibitor) into the migration medium with AGEs could hinder the binding between AGEs and AGE receptor (RAGE) located on the neutrophil, thereby keeping the normal chemotaxis of neutrophils than the ones incubated with AGEs alone. These results revealed the negative effects of high concentrations of glucose and AGEs on the neutrophil chemotaxis, suggesting that patients with diabetes should manage serum AGEs and also pay attention to blood glucose indexes. Overall, this novel microfluidic device could significantly characterize the chemotaxis of neutrophils and have the potential to be further improved into a tool for risk stratification of diabetes mellitus.
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Almeida, Joyce. "Furry friend or foe?" Medical Humanities 38, no. 1 (October 28, 2011): 61–62. http://dx.doi.org/10.1136/medhum-2011-010127.
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Arriola, Laura L., Mary K. Hausbeck, John Rogers, and Gene R. Safir. "The Effect of Trichoderma harzianum and Arbuscular Mycorrhizae on Fusarium Root Rot in Asparagus." HortTechnology 10, no. 1 (January 2000): 141–44. http://dx.doi.org/10.21273/horttech.10.1.141.
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Commercially available biocontrol agents Trichoderma harzianum Rifai and the arbuscular mycorrhizal fungus Glomus intraradices Schenck and Smith were tested for their efficacy in controlling fusarium root rot in potted asparagus (Asparagus officinalis L.) seedlings. High and low concentrations of Fusarium oxysporum (Schlect.) emend. Snyd. & Hans. f. sp. asparagi Cohen & Heald (FOA) were combined with G. intraradices and/or T. harzianum treatments. In both experiments included in this study, T. harzianum and G. intraradices alone and in combination effectively reduced root rot caused by FOA when asparagus seedlings were grown in low levels of FOA-infested medium. When seedlings were grown in high levels of FOA-infested medium, the combination of T. harzianum + G. intraradices significantly increased dry shoot mass and limited root rot compared to the control.
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Rusli, Iqna Khayatina, Arif Wibowo, and Christanti Sumardiyono. "Viabilitas dan Virulensi Fusarium oxysporum f. sp. cubense yang Dipreservasi dengan Liofilisasi." Jurnal Perlindungan Tanaman Indonesia 22, no. 2 (November 15, 2018): 167. http://dx.doi.org/10.22146/jpti.25715.
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Wilt disease caused by Fusarium oxysporum f. sp. cubense (Foc) is still pose as constraint to banana production all over the world. The objective of this research was to examine the viability and virulence of Foc isolates which had been preserved with lyophilization for 18 years. The experiment used 19 isolates of Foc, which were Pekulo, Sukorejo, Kali Sepanjang, Kalibaru, Purwojoyo, Prembun, Mulyosari, Sidogede, Sidatan, Kemiri, Juwangen, A-17, A-11, PS-5, B4-3-1, Fo2.16.16., PS-10, A-2, and Irja. The results showed that 12 isolates could grow well on PDA medium, namely Pekulo, Sukorejo, Kali Sepanjang, Kalibaru, Purwojoyo, Prembun, Mulyosari, Sidatan, Juwangen, PS-5, B4-3-1, and Fo2.16.16. Meanwhile, seven isolates (Sidogede, Kemiri, A-17, A-11, PS-10, A-2, and Irja) could not grow or develop on PDA medium. The result of pathogenicity test showed that six tested Foc isolates had very high virulences, with disease severity index of about 2.14–2.71. Other three isolates revealed high virulences with disease severity index around 2.01–2.07. Meanwhile, another three less virulent isolates demonstrated disease severity index in the range of 1.39–1.67. Intisari Penyakit layu yang disebabkan oleh jamur Fusarium oxysporum f. sp.cubense (Foc) masih menjadi kendala dalam produksi pisang di seluruh dunia. Tujuan dari penelitian ini adalah untuk menguji viabilitas dan virulensi isolat Foc yang telah dipreservasi dengan liofilisasi selama 18 tahun. Penelitian menggunakan 19 isolat Foc yaitu Pekulo, Sukorejo, Kali Sepanjang, Kalibaru, Purwojoyo, Prembun, Mulyosari, Sidogede, Sidatan, Kemiri, Juwangen, A-17, A-11, PS-5, B4-3-1, Fo2.16.16., PS-10, A-2, dan Irja. Hasil penelitian menunjukkan bahwa 12 isolat Foc mampu tumbuh dengan baik pada medium PDA yaitu Pekulo, Sukorejo, Kali Sepanjang, Kalibaru, Purwojoyo, Prembun, Mulyosari, Sidatan, Juwangen, PS-5, B4-3-1, dan Fo2.16.16. 7 isolat tidak mampu tumbuh pada medium PDA yaitu Sidogede, Kemiri, A-17, A-11, PS-10, A-2, dan Irja. Enam isolat Foc yang diuji memiliki virulensi sangat tinggi dengan indeks keparahan penyakit berkisar antara 2,14–2,71. Tiga isolat yang lain memiliki virulensi tinggi dengan indeks keparahan penyakit berkisar antara 2,01–2,07. Sedangkan 3 isolat lainnya memiliki virulensi yang lebih rendah dengan indeks keparahan penyakit berkisar antara 1,39–1,67.
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KATO, Fumitaka, Yuji MORI, Shiroh ITAI, and Yoshiyuki MIWA. "Massive fog media." Proceedings of Mechanical Engineering Congress, Japan 2016 (2016): S1210102. http://dx.doi.org/10.1299/jsmemecj.2016.s1210102.
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Alex, Alice, and Zoltán Zajacz. "A new method to quantitatively control oxygen fugacity in externally heated pressure vessel experiments." European Journal of Mineralogy 32, no. 1 (February 24, 2020): 219–34. http://dx.doi.org/10.5194/ejm-32-219-2020.
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Abstract. Oxygen fugacity (fO2) is a fundamental variable affecting phase equilibrium in magmas, and in externally heated pressure vessel experiments it is typically controlled by using redox buffer assemblages. However, these do not allow fine enough resolution; for example, most arc magmas fall between the fO2 imposed by the neighboring Ni–NiO and Re–ReO2 buffers and so does the transition of S2− to S6+ in magmas. Here we propose a new method to quantitatively impose fO2 in hydrous high-P–T experiments in molybdenum hafnium carbide (MHC) pressure vessels by admixing small amounts of hydrogen into the Ar pressure medium. The thermodynamic calculation procedure used to determine the initial amount of hydrogen to be loaded to constrain desired fO2 values was verified by CoPd alloy redox sensor experiments to be accurate within ±0.3 log units for the pressure (P) – temperature (T) range of 940–2060 bar and 800–1100 ∘C. As hydrogen can be slowly lost from the pressure medium due to diffusion through the vessel walls at high T, we also determined the hydrogen permeability of the MHC alloy as a function of T. The such-obtained hydrogen permeability equation for the MHC alloy can be used to determine the rate of fO2 increase for any MHC pressure vessel configuration. As the rate of fO2 increase is slow (e.g., 0.36 log units per day in our setup at T= 1000 ∘C), we propose that H2 addition to the Ar pressure medium is an effective way to accurately impose fO2 in many types of experiments conducted in MHC vessels allowing experimentation up to T= 1200 ∘C and P= 300 MPa.
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Lee, Jang Hee, Ji Ung Na, Dong Hyuk Shin, Pil Cho Choi, Sang O. Park, Won Jae Kim, and Sang Kuk Han. "Utility of a Modified Oropharyngeal Airway for Performing Tracheal Intubation Using a Fiberoptic Bronchoscope and Video Stylet: A Randomized Crossover Trial Using a Manikin." Emergency Medicine International 2020 (October 29, 2020): 1–7. http://dx.doi.org/10.1155/2020/3017297.
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Purpose. The purpose of this study was to assess if a modified airway (MA), developed by the authors, would act as a guide and improve the performance of intubation when used with a video stylet (VS) or fiberoptic bronchoscope (FOB) for endotracheal intubation. Methods. This randomized crossover simulation study using manikins was conducted with 36 novice operators. Time to complete intubation, time to see the glottis, and success rate of intubation of each device were measured and compared with or without use of MA. Results. For intubation using FOB with MA, the median time to complete intubation significantly reduced from 46 to 31 seconds with a medium effect size ( p = 0.004 , r = 0.483), and the median time to see the glottis significantly reduced from 7 to 5 seconds with a medium effect size ( p = 0.032 , r = 0.357). The overall success rate was not statistically different between FOB with MA (33/36, 91.7%) and FOB alone (31/36, 86.1%); however, the cumulative success rate over time for FOB with MA was higher than that for FOB alone ( p = 0.333 ). For intubation using VS, there were no differences in the time to see the glottis and time to complete intubation between VS with MA and VS alone ( p = 0.065 and p = 0.926 , respectively), and the cumulative success rate was not statistically significant ( p = 0.594 ). Conclusion. Adjunct use of MA helped reduce time to complete intubation in FOB, but not in VS. If an inexperienced operator uses FOB, it would be helpful to use MA as an adjunct device.
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Sembiring, Albert, and Natalia Lusianingsih Sumanto. "ISOLATION OF ANTIFUNGI BACTERIA FROM BANANA RHIZOSPHERE TO INHIBIT Fusarium Oxysproum f.sp cubense (FOC) GROWTH." JURNAL BIOSAINS 5, no. 3 (December 2, 2019): 105. http://dx.doi.org/10.24114/jbio.v5i3.15293.
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Fusarium wilt disease on banana has been known as panama disease one of the main diseases that cause huge losses for banana farmers. It is caused by the soil-borne fungal pathogen, Fusarium oxysporum f.sp cubense (Foc), which is very hard control because it is saprophytic in the soil. The mold infiltrates the root to vascular tissue that induces yellowing on the leaf, so this pathogen can attack the root, stem dan leaf. The research aimed to search bacteria from the banana rhizosphere that have an antifungal activity to inhibit Foc growth. Bacteria was isolated by serial dilution then was spread on King’s B agar medium incubation 28oC (room temperature). Four quadrants in vitro test on PDA medium used twenty bacterial from isolation, from the test was obtained six isolates have the potential to inhibit the growth of Foc. Based on percentage inhibition radial growth four isolates that have inhibition 50% over which TR2 was the highest at 79.07%. The in vitro test confirmed that bacteria from the banana rhizosphere have potential as biocontrol agent because it was able to inhibit the Foc growth.
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Teshima, Takahiro, Yuyo Yasumura, Ryohei Suzuki, and Hirotaka Matsumoto. "Antiviral Effects of Adipose Tissue-Derived Mesenchymal Stem Cells Secretome against Feline Calicivirus and Feline Herpesvirus Type 1." Viruses 14, no. 8 (July 30, 2022): 1687. http://dx.doi.org/10.3390/v14081687.
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Mesenchymal stem cells (MSCs) have excellent anti-inflammatory and immunomodulatory capabilities and therapeutic effects in some viral diseases. The therapeutic impact of MSCs mainly relies on the paracrine effects of various secreted substances. Feline calicivirus (FCV) and feline herpesvirus type 1 (FHV1) are common and highly prevalent pathogens causing upper respiratory diseases, and FCV is associated with gingivostomatitis in cats. Recently, feline MSC treatment has been reported to improve the clinical symptoms of feline chronic gingivostomatitis, but the antiviral effects of feline MSCs on FCV and FHV1 are not known. In this study, we evaluated the antiviral efficacy of using feline MSC secretome as a conditioned medium on FCV and FHV1 viral replication in Crandell–Reese feline kidney (CRFK) cells, and RNA sequencing was used to analyze how the CRFK cells were altered by the MSC secretomes. The feline MSC secretome did not inhibit FCV or FHV1 viral entry into the CRFK cells but had antiviral effects on the replication of both FCV and FHV1 in a dose-dependent manner.
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Al-Wahaibi, Lamya H., Muneera S. M. Al-Saleem, Osama A. A. Ahmed, Usama A. Fahmy, Nabil A. Alhakamy, Basma G. Eid, Ashraf B. Abdel-Naim, Wael M. Abdel-Mageed, Maha M. AlRasheed, and Gamal A. Shazly. "Optimized Conjugation of Fluvastatin to HIV-1 TAT Displays Enhanced Pro-Apoptotic Activity in HepG2 Cells." International Journal of Molecular Sciences 21, no. 11 (June 10, 2020): 4138. http://dx.doi.org/10.3390/ijms21114138.
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Accumulating evidence indicates that statins reduce the risk of different cancers and inhibit the proliferation of liver cancer cells. This study aims to explore whether the electrostatic conjugation of optimized fluvastatin (FLV) to human immunodeficiency virus type 1 (HIV-1) trans-activator transcription peptide (TAT) would enhance the anti-proliferative activity against HepG2 cells. FLV–TAT conjugation was optimized to achieve the lowest size with highest zeta potential. Nine formulae were constructed, using a factorial design with three factors—FLV concentration, TAT concentration, and pH of the medium—while the responses were zeta potential and size. The optimized formula showed a particle size of 199.24 nm and 29.14 mV zeta potential. Data indicates that conjugation of FLV to TAT (optimized formula) significantly enhances anti-proliferative activity and uptake by HepG2 cells when compared to raw FLV. Flow cytometry showed significant accumulation of cells in the pre-G phase, which highlights higher apoptotic activity. Annexin V staining indicated a significant increase in total cell death in early and late apoptosis. This was confirmed by significantly elevated caspase 3 in cells exposed to FLV–TAT preparation. In conclusion, the FLV–TAT optimized formula exhibited improved anti-proliferative action against HepG2. This is partially attributed to the enhanced apoptotic effects and cellular uptake of FLV.
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Martínez, Gustavo, Barlin O. Olivares, Juan Carlos Rey, Juan Rojas, Jaime Cardenas, Carlos Muentes, and Carolina Dawson. "The Advance of Fusarium Wilt Tropical Race 4 in Musaceae of Latin America and the Caribbean: Current Situation." Pathogens 12, no. 2 (February 8, 2023): 277. http://dx.doi.org/10.3390/pathogens12020277.
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The fungus Fusarium oxysporum f. sp. cubense tropical race 4 (syn. Fusarium odoratissimum) (Foc TR4) causes vascular wilt in Musaceae plants and is considered the most lethal for these crops. In Latin America and the Caribbean (LAC), it was reported for the first time in Colombia (2019), later in Peru (2021), and recently declared in Venezuela (2023). This work aimed to analyze the evolution of Foc TR4 in Musaceae in LAC between 2018 and 2022. This perspective contains a selection of topics related to Foc TR4 in LAC that address and describe (i) the threat of Foc TR4 in LAC, (ii) a bibliometric analysis of the scientific production of Foc TR4 in LAC, (iii) the current situation of Foc TR4 in Colombia, Peru, and Venezuela, (iv) medium-term prospects in LAC member countries, and (v) export trade and local food security. In this study, the presence of Foc TR4 in Venezuela and the possible consequences of the production of Musaceae in the long term were reported for the first time. In conclusion, TR4 is a major threat to banana production in Latin America and the world, and it is important to take measures to control the spread of the fungus and minimize its impact on the banana industry. It is important to keep working on the control of Foc TR4, which requires the participation of the local and international industry, researchers, and consumers, among others, to prevent the disappearance of bananas.
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Turkin, A. M., E. L. Pogosbekyan, A. C. Tonoyan, E. I. Shults, I. I. Maximov, M. B. Dolgushin, N. V. Khachanova, et al. "Diffusion Kurtosis Imaging in the Assessment of Peritumoral Brain Edema in Glioblastomas and Brain Metastases." Medical Visualization, no. 4 (August 28, 2017): 97–112. http://dx.doi.org/10.24835/1607-0763-2017-4-97-112.
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Aim: to explore the opportunities of application of diffusionkurtosis imaging (DKI) for assessment and estimation of diffusion scalar metrics in different locations of peritumoral edema for extra- and intracerebral tumors and in contralateral normal tissue.Materials and methods. 38 patients with supratentorial brain tumors were investigated: 24 (63%) patients with primarily revealed glioblastomas (GB) and 14 (37%) patients with solitary cancer brain metastasis (MTS). MRI was performed on 3.0 T MR-scanner (Signa HDxt, General Electric, USA) with the standard protocols for brain tumor and additional protocol for DKI. The standard protocol for brain tumor included: T1-, T2-weighted images, T2-FLAIR, DWI, T1 with contrast enhancement. Diffusion kurtosis MRI based on SE EPI with TR = 10000 ms, TE = 102 ms, FOV = 240 mm, isotropic voxel size 3 × 3 × 3 mm3, 60 noncoplanar diffusion directions. We used three b-values: 0, 1000 and 2500 s/mm2. Аcquisition time was 22 min. Total acquisition time was near 40 min. This study was approved by Ethical committee of Burdenko National Scientific and Practical Center for Neurosurgery. Parametric maps were constructed for the following diffusion coefficients: mean (MK), transverse / radial (RK), longitudinal / axial (AK) kurtozis; medium (MD), transverse / radial (RD) and longitudinal / axial (AD) diffusion; fractional anisotropy (FA) and a bi-exponential diffusion model coefficients: axonal water fractions (AWF), axial (AxEAD) and radial (RadEAD) extra-axonal water diffusion and the water molecules trajectory tortuosity index (TORT). Normative quantitative indicators were obtained for the six regions of the peritumoral zone as they moved away from the tumor (region 2) to the edema periphery (regions 4–5), as well as in the normal brain on the contralateral hemisphere (C/L) (zone 7). A comparative analysis of these indicators was conducted for cases with GB and MTS. DKI scalar metrics were estimated using Explore DTI (http://www.exploredti.com/).Results. Anatomic MRI (T1 without/with contrast enhancement) for all cases with GB and MTS visualized a contrast enhancement tumor. The peritumoral edema, spreading mainly over the brain white matter, was well visualized on T2-FLAIR. Diffusion kurtosis coefficients decreased in the near peritumoral edema (regions 2–3) and a gradually increased to the edema periphery (regions 5–6). In Region 2, MK in both GB and MTS groups were MKGB(2) = 0.637 ± 0.140 and MKMTS(2) = 0.550 ± 0.046; RK in this region were RKGB(2) = 0.690 ± 0.154 and RKMTS (2) = 0.584 ± 0.051. Differences both MK and RK coefficients in patients with GB and MTS of region 2 were significant (p < 0.001). There were no differences in AK values for GB and MTS in region 2 (p > 0.05), but in regions 3 and 4 differences were observed (p < 0.01). The minimum value of AK in the central edema (regions 3–4) was AKMTS(3–4) = 0.433 ± 0.063 in patients with MTS. The values of MK and RK on the contralateral side in patients with MTS were significantly higher than in the GB group (p < 0.02); MKC/LMTC = 0.954 ± 0.140, RKC/LMTC = 1.257 ± 0.308 and MKC/LGB = 0.829 ± 0.146, RKc/LGB = 0.989 ± 0.282. There was no significant difference for contralateral AK between the groups.Conclusions. We found that DKI scalar metrics are the sensitive tumor biomarkers. It allows us to perform a robust differentiation between the infiltrating GB tumor and purely vasogenic edema of МТS. The obtained results will allow further differential diagnosis of extra- and intracerebral tumors and can be used to plan surgical / radiosurgical treatment for brain tumors.
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Johnston, J. B., C. Silva, and C. Power. "Envelope Gene-Mediated Neurovirulence in Feline Immunodeficiency Virus Infection: Induction of Matrix Metalloproteinases and Neuronal Injury." Journal of Virology 76, no. 6 (March 15, 2002): 2622–33. http://dx.doi.org/10.1128/jvi.76.6.2622-2633.2002.
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ABSTRACT The release of neurotoxins by activated brain macrophages or microglia is one mechanism proposed to contribute to the development of neurological disease following infection by lentiviruses, including feline immunodeficiency virus (FIV). Since molecular diversity in the lentiviral envelope gene influences the expression of host molecules implicated in neuronal injury, the role of the envelope sequence in FIV neuropathogenesis was investigated by using the neurovirulent FIV strain V1CSF, the nonneurovirulent strain Petaluma, and a chimera (FIVCh) containing the V1CSF envelope gene in a Petaluma background. All three viruses replicated in primary feline macrophages with equal efficiency, but conditioned medium from V1CSF- or FIVCh-infected cells was significantly more neurotoxic than medium from Petaluma-infected cultures (P < 0.001) and could be attenuated in a dose-dependent manner by treatment with either the matrix metalloproteinase (MMP) inhibitor prinomastat (PMT) or function-blocking antibodies to MMP-2. Although FIV sequences were detectable by PCR in brain tissue from neonatal cats infected with each of the viral strains, immunohistochemistry revealed increased astrogliosis and macrophage activation in the brains of V1CSF- and FIVCh-infected cats relative to the other groups, together with elevated markers of neuronal stress that included morphological changes and increased c-fos immunoreactivity. Similarly, MMP-2, but not MMP-9, mRNA and protein expression was increased in brain tissues of V1CSF- and FIVCh-infected cats relative to Petaluma-infected animals (P < 0.01). Infection with V1CSF or FIVCh was also associated with greater CD4+ cell depletion (P < 0.001) and neurodevelopmental delays (P < 0.005), than in Petaluma-infected animals; these deficits improved following PMT therapy. These findings indicated that diversity in the envelope gene sequence influenced the neurovirulence exhibited by FIV both in vitro and in vivo, possibly through a mechanism involving the differential induction of MMP-2.
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Barrera-Vázquez, Oscar Salvador, Clotilde Cancio-Lonches, Carlos Emilio Miguel-Rodríguez, Monica Margarita Valdes Pérez, and Ana Lorena Gutiérrez-Escolano. "Survivin Overexpression Has a Negative Effect on Feline Calicivirus Infection." Viruses 11, no. 11 (October 30, 2019): 996. http://dx.doi.org/10.3390/v11110996.
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It is known that levels of the anti-apoptotic protein survivin are reduced during Murine norovirus MNV-1 and Feline calicivirus (FCV) infection as part of the apoptosis establishment required for virus release and propagation in the host. Recently, our group has reported that overexpression of survivin causes a reduction of FCV protein synthesis and viral progeny production, suggesting that survivin may affect early steps of the replicative cycle. Using immunofluorescence assays, we observed that overexpression of survivin, resulted in the reduction of FCV infection not only in transfected but also in the neighboring nontransfected CrFK cells, thus suggesting autocrine and paracrine protective effects. Cells treated with the supernatants collected from CrFK cells overexpressing survivin showed a reduction in FCV but not MNV-1 protein production and viral yield, suggesting that FCV binding and/or entry were specifically altered. The reduced ability of FCV to bind to the surface of the cells overexpressing survivin, or treated with the supernatants collected from these cells, correlate with the reduction in the cell surface of the FCV receptor, the feline junctional adhesion molecule (fJAM) 1, while no effect was observed in the cells transfected with the pAm-Cyan vector or in cells treated with the corresponding supernatants. Moreover, the overexpression of survivin affects neither Vaccinia virus (VACV) production in CrFK cells nor MNV-1 virus production in RAW 267.4 cells, indicating that the effect is specific for FCV. All of these results taken together indicate that cells that overexpress survivin, or cell treatment with the conditioned medium from these cells, results in the reduction of the fJAM-1 molecule and, therefore, a specific reduction in FCV entry and infection.
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Segura M., Rafael A., Jetse J. Stoorvogel, Fabio A. Blanco R., and Jorge A. Sandoval F. "A Medium-Term Field Experiment to Study the Effect of Managing Soil Chemical Properties on Fusarium Wilt in Banana (Musa AAA)." Journal of Fungi 7, no. 4 (March 31, 2021): 261. http://dx.doi.org/10.3390/jof7040261.
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Fusarium oxysporum f. sp. cubense (Foc) is a soil-borne fungus causing Fusarium wilt (FW) in banana. It is practically impossible to eradicate Foc in soils. Our understanding of soil–Foc–banana interactions is hampered by inconsistent research results caused by agro-ecological variability and the complexity of the soil system. This study aimed to evaluate the options to manage soil chemical properties to reduce disease expression and maintain banana production. The expression of FW (Foc Race 1) and the agronomic performance of the Gros Michel (Musa AAA) banana were evaluated in two medium-term factorial field experiments at representative locations in the Costa Rican banana region. In the experiments, five soil chemical properties (pH, N, Ca, Mg, and Mn) were managed to achieve a low and a high level. Plant mortality caused by FW, soil fertility, plant nutrition, and agronomic performance were monitored during four crop cycles. After the first crop cycle, the treatments started to present differences in plant mortality. There was a significant rise of plant mortality after the second crop cycle resulting in a cumulative plant mortality exceeding 60% in both experiments. A lower soil pH consistently resulted in significantly higher plant mortality. The interactions between soil properties (pH-N, pH-CaMg, pH-Mn, N-Mn, and CaMg-Mn) also influenced plant mortality. Soil N was the most significant treatment affecting leaf nutrient concentrations, bunch weight, and clusters per bunch. The experiments confirmed the potential role of soil management in FW expression in banana. Our results suggest that the management of soil chemical properties in the conditions here studied may help to reduce the expression rate of FW, but not to control the disease in the long run.
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Cenesizoglu, Tolga, Qianqiu Liu, Jonathan J. Reeves, and Haifeng Wu. "Monthly Beta Forecasting with Low-, Medium- and High-Frequency Stock Returns." Journal of Forecasting 35, no. 6 (March 10, 2016): 528–41. http://dx.doi.org/10.1002/for.2396.
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Shoesmith, Gary L. "Co-integration, error correction and improved medium-term regional VAR forecasting." Journal of Forecasting 11, no. 2 (February 1992): 91–109. http://dx.doi.org/10.1002/for.3980110202.
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Dick, C. P., R. P. Johnson, T. Bast, and S. Yamashiro. "Ultrastructure of feline calicivirus-infected crandell's feline kidney (CRFK) cells in culture." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 310–11. http://dx.doi.org/10.1017/s0424820100103619.
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Viruses frequently are difficult to visualize using routine transmission electron microscopy (TEM) of tissues collected from infected animals. Using cell cultures infected with viruses as in vitro models, viral replication and their resultant cytopathic effects can be more easily examined.Feline calicivirus (FCV), a common upper respiratory pathogen in cats has been successfully propagated in Crandell's Feline Kidney (CRFK) cells and a number of ultrastructural viral inclusions noted (1-4). This study represents further investigation of FCV infected CRFK cells using strain 255. The similarities and differences in our findings as compared to those of previous investigations have been explored.Confluent monolayers of CRFK cells in 80 cm2 plastic flasks were inoculated with 105 median cell culture infectious doses of FCV strain 255. When approximately 60 percent of cells exhibit FCV cytopathic effect the medium was decanted and the cells scraped from the surface using a rubber policeman. The cell suspension was centrifuged at 5000 rpm for 20 minutes at 4°C. Details of the procedure have been reported previously (5).
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Linenberger, ML, GH Shelton, MT Persik, and JL Abkowitz. "Hematopoiesis in asymptomatic cats infected with feline immunodeficiency virus." Blood 78, no. 8 (October 15, 1991): 1963–68. http://dx.doi.org/10.1182/blood.v78.8.1963.1963.
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Abstract Anemia and neutropenia often develop in cats that are infected with the feline immunodeficiency virus (FIV), a lentivirus biologically similar to the human immunodeficiency virus (HIV). To assess the role of FIV in the pathogenesis of these abnormalities, marrow culture studies were performed on nine asymptomatic, hematologically normal cats that were chronically infected with FIV. In these experiments, the frequencies of granulocyte/macrophage progenitors (CFU-GM) and early and late erythroid progenitors (CFU-E and BFU-E, respectively) were equivalent to progenitor frequencies in simultaneously studied uninfected control cats. Asymptomatic FIV infection was not associated with a change in the cell-cycle kinetics of CFU-E, BFU-E, or CFU-GM, nor was there an alteration in the dose-response of BFU-E or CFU-GM to hematopoietic growth factors present in fibroblast-derived conditioned medium. Sera from FIV-infected cats supported progenitor growth in vitro as well as normal cat sera. Furthermore, there was no evidence that these sera contained complement-fixing antibodies that recognized hematopoietic progenitors. Therefore, these data show that the in vitro behavior of hematopoietic progenitors is not affected by FIV infection alone, and they are in agreement with recent evidence that human progenitors are not a major target of HIV infection. It is likely that factors associated with progressive immunodeficiency, opportunistic infections, nutritional deficiencies, or malignancies play significant roles in the cytopenias that develop during the symptomatic disease induced by FIV, and by analogy, HIV. Prospective marrow culture studies of FIV-infected cats that develop hematologic abnormalities should provide a valuable animal model of acquired immunodeficiency syndrome-associated hematologic disorders.
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Linenberger, ML, GH Shelton, MT Persik, and JL Abkowitz. "Hematopoiesis in asymptomatic cats infected with feline immunodeficiency virus." Blood 78, no. 8 (October 15, 1991): 1963–68. http://dx.doi.org/10.1182/blood.v78.8.1963.bloodjournal7881963.
Full textAbstract:
Anemia and neutropenia often develop in cats that are infected with the feline immunodeficiency virus (FIV), a lentivirus biologically similar to the human immunodeficiency virus (HIV). To assess the role of FIV in the pathogenesis of these abnormalities, marrow culture studies were performed on nine asymptomatic, hematologically normal cats that were chronically infected with FIV. In these experiments, the frequencies of granulocyte/macrophage progenitors (CFU-GM) and early and late erythroid progenitors (CFU-E and BFU-E, respectively) were equivalent to progenitor frequencies in simultaneously studied uninfected control cats. Asymptomatic FIV infection was not associated with a change in the cell-cycle kinetics of CFU-E, BFU-E, or CFU-GM, nor was there an alteration in the dose-response of BFU-E or CFU-GM to hematopoietic growth factors present in fibroblast-derived conditioned medium. Sera from FIV-infected cats supported progenitor growth in vitro as well as normal cat sera. Furthermore, there was no evidence that these sera contained complement-fixing antibodies that recognized hematopoietic progenitors. Therefore, these data show that the in vitro behavior of hematopoietic progenitors is not affected by FIV infection alone, and they are in agreement with recent evidence that human progenitors are not a major target of HIV infection. It is likely that factors associated with progressive immunodeficiency, opportunistic infections, nutritional deficiencies, or malignancies play significant roles in the cytopenias that develop during the symptomatic disease induced by FIV, and by analogy, HIV. Prospective marrow culture studies of FIV-infected cats that develop hematologic abnormalities should provide a valuable animal model of acquired immunodeficiency syndrome-associated hematologic disorders.
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M.M., Ubaydullaev, and Komilov J.N. "EFFECT OF DEFOLIANTS FOR MEDIUM FIBER COTTON." International Journal of Advance Scientific Research 02, no. 05 (May 1, 2022): 1–5. http://dx.doi.org/10.37547/ijasr-02-05-01.
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García, Víctor, Carmen Márquez, Andrés Cedeño, and Kleber Montesdeoca. "Assessing Bioremediation of Soils Polluted with Fuel Oil 6 by Means of Diffuse Reflectance Spectroscopy." Resources 8, no. 1 (February 13, 2019): 36. http://dx.doi.org/10.3390/resources8010036.
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This study aimed to assess the bioremediation of soils polluted with fuel oil 6 (FO6) using diffuse reflectance (DR) spectroscopy in the visible and near infrared (Vis-NIR) electromagnetic spectrum. To achieve our goal, we determined the spectral signature of fuel oil 6 (FO6), developed a calibration model to quantify the total petroleum hydrocarbons (TPH), and assessed the bioremediation in soils contaminated with FO6 and inoculated with Pseudomonas aeruginosa. Surface soil samples (SS) (0–30 cm depth) from uncontaminated Entisol soil from Termoesmeraldas Thermal Power Plant, Ecuador and quart sand (QS) samples were spiked with FO6 at a known contamination of 0.5, 1, 3, 6, 9, 12 wt.% on a gravimetric basis. A sample of contaminated Entisol soil was taken to isolate P. aeruginosa from a spill site located in Termoesmeraldas. P. aeruginosa was successfully augmented in a molasses medium. The results suggested that the C–H stretch combination overtone band around 2300 nm is the one that makes the significant contribution to the FO6 spectral signature and for the analysis of FO6 contaminated Entisols soil. The calibration model for QS samples and SS showed an excellent agreement with experimental data R2 = 0.9989 and R2 = 0.9968, respectively. The TPH at 0, 7, 14, 21, and 23 days after inoculation were found using a calibration model developed and the Unach hydrocarbon index (UHI). While the QS samples showed the lower recovery rate (13.6%), the Entisols SS showed the higher recovery rate (45.8%) in 23 days. The use of DR spectroscopy and determination of the FO6 spectral signature allowed the assessment of the bioremediation process of QS and Entisols SS samples. The results showed that DR decreased with increasing the FO6 concentration and soil properties affected the degree of biodegradation.
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Lindh, Thomas. "Medium-term forecasts of potential GDP and inflation using age structure information." Journal of Forecasting 23, no. 1 (January 2004): 19–49. http://dx.doi.org/10.1002/for.906.
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Maitra, A., and Marina Dan. "Propagation of pulses at optical wavelengths through fog-filled medium." Radio Science 31, no. 3 (May 1996): 469–75. http://dx.doi.org/10.1029/95rs02451.
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Weathers, Kathleen C., Alexandra G. Ponette-González, and Todd E. Dawson. "Medium, Vector, and Connector: Fog and the Maintenance of Ecosystems." Ecosystems 23, no. 1 (May 6, 2019): 217–29. http://dx.doi.org/10.1007/s10021-019-00388-4.
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KINGSLEY, DAVID H., and HAIQIANG CHEN. "Aqueous Matrix Compositions and pH Influence Feline Calicivirus Inactivation by High Pressure Processing†." Journal of Food Protection 71, no. 8 (August 1, 2008): 1598–603. http://dx.doi.org/10.4315/0362-028x-71.8.1598.
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The individual effects of pH (pH 3 to 8), NaCl (0 to 21%), sucrose (0 to 70%), and whey protein (0 to 2%) on pressure resistance of feline calicivirus (FCV) in Dulbecco's modified Eagle medium with 10% fetal bovine serum were determined. At pH 3 through 8, the virus was more resistant to pressure at a pH of ≤5.2. For FCV samples with sucrose (up to 40%) or NaCl (up to 12%), the amount of FCV inactivated by pressure was inversely proportional to the sucrose or NaCl concentration. For example, a treatment of 250 MPa at 20°C for 5 min reduced the FCV titer by 5.1 log PFU/ml without added sucrose and by 0.9 log PFU/ml with 40% sucrose. Reduced pressure sensitivity with increasing NaCl and sucrose concentrations was not a simple function of water activity. Different PFU reductions were observed for NaCl and sucrose samples with equivalent water activity. Whey protein at concentrations up to 2% did not provide a protective effect. The combined effect of NaCl and sucrose at 4 and 20°C on pressure resistance of FCV also was examined. When both NaCl and sucrose were added to the FCV stock, they had an additive effect on increasing the pressure resistance of FCV. The individual (6% NaCl or 20% sucrose) and combined (6% NaCl plus 20% sucrose) resistance effects did not abrogate enhanced inactivation for pressure treatments at 4°C compared with those at 20°C. Aqueous matrix compositions, in particular different concentrations of NaCl and sucrose or different pH values, can substantially alter the efficiency of virus inactivation by high pressure processing.