Journal articles on the topic 'Medical and biomedical engineering, n.e.c'

The mechanically important constituents of swelling tissues are fibers embedded in an osmotically active fluid. The tissues’ response to external loading is the sum of contribution of the axial stresses in the fibers and of the fluid pressure. The fluid osmotic properties play a key role in determining its equilibrium response. The present study examines the conditions under which the elastic response of tissues as modeled by structural constitutive equations, is thermodynamically plausible. The analysis shows that plausibility is ensured if the fibers’ axial force increases monotonically with stretch and if the fluid osmotic pressure increases convexly with concentration. Published data shows that both conditions prevail in swelling tissues. Plausibility considerations seem to pose no specific restrictions on the structure of the tissues’ fibrous network. It is thus concluded that in swelling tissues, structural constitutive formulation is compatible with thermodynamically plausible response.

Successful improvement of cryopreservation protocols for cells in suspension requires knowledge of how such cells respond to the biophysical stresses of freezing (intracellular ice formation, water transport) while in the presence of a cryoprotective agent (CPA). This work investigates the biophysical water transport response in a clinically important cell type—isolated hepatocytes—during freezing in the presence of dimethylsulfoxide (DMSO). Sprague-Dawley rat liver hepatocytes were frozen in Williams E media supplemented with 0, 1, and 2 M DMSO, at rates of 5, 10, and 50°C/min. The water transport was measured by cell volumetric changes as assessed by cryomicroscopy and image analysis. Assuming that water is the only species transported under these conditions, a water transport model of the form dV/dT = f(Lpg([CPA]), ELp([CPA]), T(t)) was curve-fit to the experimental data to obtain the biophysical parameters of water transport—the reference hydraulic permeability (Lpg) and activation energy of water transport (ELp)—for each DMSO concentration. These parameters were estimated two ways: (1) by curve-fitting the model to the average volume of the pooled cell data, and (2) by curve-fitting individual cell volume data and averaging the resulting parameters. The experimental data showed that less dehydration occurs during freezing at a given rate in the presence of DMSO at temperatures between 0 and −10°C. However, dehydration was able to continue at lower temperatures (<−10°C) in the presence of DMSO. The values of Lpg and ELp obtained using the individual cell volume data both decreased from their non-CPA values—4.33 × 10−13 m3/N-s (2.69 μm/min-atm) and 317 kJ/mol (75.9 kcal/mol), respectively—to 0.873 × 10−13 m3/N-s (0.542 μm/min-atm) and 137 kJ/mol (32.8 kcal/mol), respectively, in 1 M DMSO and 0.715 × 10−13 m3/N-s (0.444 μm/min-atm) and 107 kJ/mol (25.7 kcal/mol), respectively, in 2 M DMSO. The trends in the pooled volume values for Lpg and ELp were very similar, but the overall fit was considered worse than for the individual volume parameters. A unique way of presenting the curve-fitting results supports a clear trend of reduction of both biophysical parameters in the presence of DMSO, and no clear trend in cooling rate dependence of the biophysical parameters. In addition, these results suggest that close proximity of the experimental cell volume data to the equilibrium volume curve may significantly reduce the efficiency of the curve-fitting process.

The sensitive monitoring of dopamine levels in the human body is of utmost importance since its abnormal levels can cause a variety of medical and behavioral problems. In this regard, we report the synthesis of nitrogen-doped graphene quantum dots (N-GQDs) from polyindole (PIN) via a facile single-step hydrothermal synthetic strategy that can act as an efficient electrochemical catalyst for the detection of dopamine (DA). The average diameter of N-GQDs was ∼5.2 nm and showed a C/N atomic ratio of ∼2.75%. These N-GQDs exhibit a cyan fluorescence color under irradiation from a 365 nm lamp, while PIN has no characteristic PL. The presence of richly N-doped graphitic lattices in the N-GQDs possibly accounts for the improved catalytic activity of N-GQDs/GCE towards electrocatalytic DA detection. Under optimum conditions, this novel N-GQDs-modified electrode exhibits superior selectivity and sensitivity. Moreover, it could detect as low as 0.15 nM of DA with a linear range of 0.001–1000 µM. In addition, the outstanding sensing attributes of the detector were extended to the real samples as well. Overall, our findings evidence that N-GQDs-based DA electrochemical sensors can be synthesized from PIN precursor and could act as promising EC sensors in medical diagnostic applications.

Background: The evidence in the medical literature on the treatment of hepatitis C virus–associated glomerular disease is extremely limited. The advent of nonconventional immunosuppressive agents and direct-acting antivirals promises high efficacy and safety. Aims: We conducted an open-label, single-arm clinical study to examine the efficacy and safety of a combined approach for hepatitis C virus–associated glomerular disease. Methods: In the first phase of the study, patients with hepatitis C virus–associated glomerular disease received interferon-based antiviral therapy and immunosuppressive agents; since 2013, interferon-free antiviral therapy was adopted and novel immunosuppressants (including B-cell depleting agents and mycophenolate mofetil) or immunomodulators (ribavirin) were choiced. Virological and clinical responses were evaluated over a long observation period (median follow-up of 60 weeks and 46.5 months after the end of treatment with interferon and direct-acting antiviral agents, respectively). Results: We enrolled 25 consecutive patients with hepatitis C virus–associated glomerular disease, 8 being liver transplant recipients for hepatitis C. A total of 13 patients received therapy with direct-acting antivirals and experienced sustained viral response (serum hepatitis C virus RNA <12 IU/mL, 12 weeks after treatment ended, sustained viral response12). The mean (±standard deviation) proteinuria decreased from 2.61 ± 1.01 at baseline to 1.71 ± 1.43 (g/day) at sustained viral response 48, p = 0.031; microscopic hematuria and serum cryoglobulins disappeared in six (50%) and seven (64%) patients, respectively, after sustained viral response by direct-acting antivirals. Adverse events occurred in 69% (9/13) of patients and were mild, with four cases of ribavirin-related anemia requiring blood transfusions (no drop-outs). After sustained viral response by direct-acting antivirals, immunosuppressive and immunomodulatory agents were initiated in clinical relapsers ( n = 2) and nonresponders ( n = 3) with some benefit. Among patients on interferon-based regimens ( n = 12), viral response (sustained viral response 24) and dropout rates were 58% (7/12) and 33% (4/12), respectively. After sustained viral response by interferon-based therapy, clinical relapsers ( n = 3) were successfully managed with immunosuppressive agents in two patients. Conclusion: Treatment with direct-acting antivirals provides excellent rates of viral response and safety in patients with hepatitis C virus–related glomerular disease; viral response was frequently accompanied by clinical improvement. The absence of hepatitis C virus RNA from serum allowed immunosuppressive and immunomodulatory therapies with benefits for glomerular abnormalities and no concern on hepatitis C virus replication.

Rhelogical measurements on a dilute suspension of red blood cells (RBCs) are interpreted by means of a microheological model that relates the shear evolution of the apparent viscosity to the intrinsic properties of the suspended particles. It is then possible to quantify the average deformability of a RBC population in terms of a mean value of the membrane shear elastic modulus, Es. Dilute suspensions of erthrocytes exhibit a shear-thinning behavior with a constant high shear viscosity. This behavior is identical to the one predicted for a suspension of spherical capsules where the same phenomena of deformation and orientation prevail. A comparison between theoretical and experimental curves yields a mean value of Es, assuming all other cell properties—internal viscosity, geometry—to be otherwise equal. In Dextran, the values of Es for normal RBCs are found to be of order 3.10−6 N/m. For erythrocytes hardened by heat exposure for 15 minutes at 48°C, the increase in Es reaches 45 percent. This procedure of shear elastic modulus determination is easy to perform and seems to give a good discrimination between normal and altered erythrocytes.

Background: Quasilinear viscoelasticity (QLV) theory has been widely and successfully used to describe the time-dependent response of connective tissues. Difficulties remain, however, particularly in material parameter estimation and sensitivities. In this study, we introduce a new alternative: the fractional order viscoelasticity (FOV) theory, which uses a fractional order integral to describe the relaxation response. FOV implies a fractal-like tissue structure, reflecting the hierarchical arrangement of collagenous tissues. Method of Approach: A one-dimensional (1-D) FOV reduced relaxation function was developed, replacing the QLV “box-spectrum” function with a fractional relaxation function. A direct-fit, global optimization method was used to estimate material parameters from stress relaxation tests on aortic valve tissue. Results: We found that for the aortic heart valve, FOV had similar accuracy and better parameter sensitivity than QLV, particularly for the long time constant (τ2). The mean (n=5) fractional order was 0.29, indicating that the viscoelastic response of the tissue was strongly fractal-like. Results summary: mean QLV parameters were C=0.079, τ1=0.004, τ2=76, and mean FOV parameters were β=0.29, τ=0.076, and ρ=1.84. Conclusions: FOV can provide valuable new insights into tissue viscoelastic behavior. Determining the fractional order can provide a new and sensitive quantitative measure for tissue comparison.

The first two authors contributed equally to this study. Bioactivity and cell adhesion properties are major factors for fabricating medical devices such as coronary stents. The aim of this study was to evaluate the advantages of atmospheric-pressure plasma jet in enhancing the biocompatibility and endothelial cell-favorites. The experimental objects were divided into before and after atmospheric-pressure plasma jet treatment with the ratio of nitrogen:argon = 3:1, which is similar to air. The treated surfaces were basically characterized by means of a contact angle analyzer for the activation property on their surfaces. The effect of atmospheric-pressure plasma jet on cellular response was examined by endothelial cell adhesion and XTT analysis. It was difficult to detect any changeable morphology after atmospheric-pressure plasma jet treatment on the surface. The roughness was increased after atmospheric-pressure plasma jet treatment compared to nonatmospheric-pressure plasma jet treatment (86.781 and 7.964 nm, respectively). The X-ray photoelectron spectroscopy results showed that the surface concentration of the C–O groups increased slightly from 6% to 8% after plasma activation. The contact angle dramatically decreased in the atmospheric-pressure plasma jet treated group (22.6 ± 15.26°) compared to the nonatmospheric-pressure plasma jet treated group (72.4 ± 15.26°) ( n = 10, p < 0.05). The effect of the increment in hydrophilicity due to the atmospheric-pressure plasma jet on endothelial cell migration and proliferation was 85.2% ± 12.01% and 34.2% ± 2.68%, respectively, at 7 days, compared to the nonatmospheric-pressure plasma jet treated group (58.2% ± 11.44% in migration, n = 10, p < 0.05). Taken together, the stent surface could easily obtain a hydrophilic property by the atmospheric-pressure plasma jet method. Moreover, the atmospheric-pressure plasma jet might affect re-endothelialization after stenting.

The over prescribing of broad-spectrum antibiotics has given rise to the multidrug resistance in bacteria. The antimicrobial resistance caused the medicines and treatments to become ineffective and became difficult to treat infection. In order to avoid the overuse of antibiotics, antibacterial material have been developed and applied in preventing bacterial infection, drug delivery, and medical implant. Nanomaterials materials show great potential in many applications [1-33]. Therefore, in our study, we designed nanosilica islands on graphene oxide template to disperse the silver nanoparticles and improve its antibacterial properties. Silver nanoparticles have been known for its exceptional antimicrobial properties. However, some bacteria were able to use their flagellum to cause the silver nanoparticles agglomerated. By using silica nanoparticles and graphene oxide, we not only avoid the agglomerated issues but also improve the antibacterial ability of the material. Our novel composite was characterized by using Powder X-Ray Diffraction (P-XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS), Tranmission electron microscopy (TEM), and Fourier Transform Infrared (FTIR). The silica nanoparticles and graphene template helped to form dispersed nanoparticles of 7 nm. The composite successfully eliminated 99.99% of Escherichia coli and Bacillus Subtilis within one hour. References: ME Mahmoud, SS Haggag, MA Rafea, TM Abdel-Fattah, Polyhedron 28 (16), 3407-3414 (2009) C Huff, JM Long, A Aboulatta, A Heyman, TM Abdel-Fattah, ECS Journal of Solid State Science and Technology 6 (10), M115 (2017) A Wixtrom, J Buhler, T Abdel-Fattah, Journal of Chemical Education 91 (8), 1232-1235 (2014) ME Mahmoud, SS Haggag, TM Abdel-Fattah, Polyhedron 26 (14), 3956-3962 (2007) C Huff, T Dushatinski, A Barzanji, N Abdel-Fattah, K Barzanji, ECS Journal of Solid State Science and Technology 6 (5), M69 (2017) TM Abdel-Fattah, B Bishop, Journal of Environmental Science and Health, Part A 39 (11-12), 2855-2866 (2014) Quach, E. Biehler, A. Elzamzami, C. Huff, J.M. Long, T.M. Abdel Fattah, Catalysts, 11, 118 (2021). Biehler, Q. Quach, C. Huff, T. M. Abdel-Fattah, Materials, 15, 2692 (2022). TM Abdel-Fattah, ME Mahmoud, MM Osmam, SB Ahmed, Journal of Environmental Science and health, part A 49 (9), 1064-1076 (2014) ME Mahmoud, TM Abdel-Fattah, MM Osman, SB Ahmed, Journal of Environmental Science and Health, Part A 47 (1), 130-141 (2012) C Huff, E Biehler, Q Quach, JM Long, TM Abdel-Fattah, Colloids and Surfaces A: Physicochemical and Engineering Aspects 610 (5), 125734 (2021) K Foe, G Namkoong, TM Abdel-Fattah, H Baumgart, MS Jeong, DS Lee, Thin solid films 534, 76-82 (2013) M Abdel-Fattah, A Wixtrom, K Zhang, W Cao, H Baumgart, ECS Journal of Solid State Science and Technology 3 (10), M61 (2014) M. Abdel Fattah, M.E. Mahmoud, S.B. Ahmed, M.D. Huff, J.W. Lee, S. Kumar, Journal of Industrial and Engineering Chemistry, 22, 103-109 (2015) M. Abdel-Fattah, M.E Mahmoud, M. M. Osmam, S.B. Ahmed, Journal of Environmental Science and health part A, 49, 1064-1076 (2014) ME Mahmoud, MA Khalifa, YM El Wakeel, MS Header, TM Abdel-Fattah, Journal of Nuclear Materials 487, 13-22 (2017) C Huff, T Dushatinski, TM Abdel-Fattah, International Journal of Hydrogen Energy 42 (30), 18985-18990 (2017) M Stacey, C Osgood, BS Kalluri, W Cao, H Elsayed-Ali, T Abdel-Fattah, Biomedical Materials 6 (1), 011002 (2011) SE Mohmed Labeb, Abdel-Hamed Sakr, Moataz Soliman, Tarek M.Abdel-Fattah, Optical Materials 79, 331-335 (2018) ME Mahmoud, MM Osman, SB Ahmed, TM Abdel-Fattah, Chemical engineering journal 175, 84-94 (2011) TM Abdel-Fattah, ME Mahmoud, Chemical engineering journal 172 (1), 177-183 (2011) R Bhure, TM Abdel-Fattah, C Bonner, JC Hall, A Mahapatro, Journal of biomedical nanotechnology 6 (2), 117-128 (2010) TM Abdel-Fattah, D Loftis, A Mahapatro, Journal of biomedical nanotechnology 7 (6), 794-800 (2011) OH Elsayed-Ali, T Abdel-Fattah, HE Elsayed-Ali, Journal of hazardous materials 185 (2-3), 1550-1557 (2011) R Bhure, A Mahapatro, C Bonner, TM Abdel-Fattah, Materials Science and Engineering: C 33 (4), 2050-2058 (2013) BE Bishop, BA Savitzky, T Abdel-Fattah, Ecotoxicology and Environmental Safety 73 (4), 565-571 (2010) C Huff, JM Long, A Heyman, TM Abdel-Fattah, ACS Applied Energy Materials 1 (9), 4635-4640 (2018) TM Abdel-Fattah, EM Younes, G Namkoong, EM El-Maghraby, Synthetic Metals 209, 348-354 (2015) S Ebrahim, M Soliman, TM Abdel-Fattah, Journal of electronic materials 40 (9), 2033-2041 (2011) SH Lapidus, A Naik, A Wixtrom, NE Massa, V Ta Phuoc, L del Campo, Crystal growth & design 14 (1), 91-100 (2014) A Mahapatro, TD Matos Negrón, C Bonner, TM Abdel-Fattah, Journal of Biomaterials and Tissue Engineering 3 (2), 196-204 (2013) S Ebrahim, M Labeb, T Abdel-Fattah, M Soliman, Journal of Luminescence 182, 154-159 (2017) T Dushatinski, C Huff, TM Abdel-Fattah, Applied Surface Science 385, 282-288 (2016)

Implant therapy using osseointegratable titanium (Ti) dental implants has revolutionized clinical dental practice and has shown a high rate of success. However, because a metallic implant is in contact with body tissues and fluids in vivo, ions/particles can be released into the biological milieu as a result of corrosion or biotribocorrosion. Ultrananocrystalline diamond (UNCD) coatings possess a synergistic combination of mechanical, tribological, and chemical properties, which makes UNCD highly biocompatible. In addition, because the UNCD coating is made of carbon (C), a component of human DNA, cells, and molecules, it is potentially a highly biocompatible coating for medical implant devices. The aim of the present research was to evaluate tissue response to UNCD-coated titanium micro-implants using a murine model designed to evaluate biocompatibility. Non-coated (n = 10) and UNCD-coated (n = 10) orthodontic Ti micro-implants were placed in the hematopoietic bone marrow of the tibia of male Wistar rats. The animals were euthanized 30 days post implantation. The tibiae were resected, and ground histologic sections were obtained and stained with toluidine blue. Histologically, both groups showed lamellar bone tissue in contact with the implants (osseointegration). No inflammatory or multinucleated giant cells were observed. Histomorphometric evaluation showed no statistically significant differences in the percentage of BIC between groups (C: 53.40 ± 13% vs. UNCD: 58.82 ± 9%, p > 0.05). UNCD showed good biocompatibility properties. Although the percentage of BIC (osseointegration) was similar in UNCD-coated and control Ti micro-implants, the documented tribological properties of UNCD make it a superior implant coating material. Given the current surge in the use of nano-coatings, nanofilms, and nanostructured surfaces to enhance the biocompatibility of biomedical implants, the results of the present study contribute valuable data for the manufacture of UNCD coatings as a new generation of superior dental implants.

Diabetes mellitus (DM) has become a major medical and health problem in my country and even the world. Doctors and patients have gradually realized that a new type of metabolic surgery is a way to treat diabetes. The operation is relatively simple, and the effect of the operation is no less than that of the gastric shunt. The initial hypothesis could not fully explain the blood pressure and blood sugar reduction mechanism in waist and abdominal surgery. According to requirements, they were divided into the sleeve gastrectomy group (SG group, n = 10) and sham operation group (SS group, n = 10), and corresponding measures were taken. Observe their weight changes; perform an oral glucose tolerance test (GB) before surgery and at 2, 8, and 16 weeks after surgery to evaluate the effect of surgery on improving the glucose metabolism. The postoperative GLP-1 specificity curve was detected in the two groups of patients; the immunohistochemical method was used to detect the postoperative changes of the digestive tract l cells in the two groups; RT-PCR was used to detect the mRNA transcription level of the digestive tract GLP-1 receptor. The bodyweight was significantly different 4 weeks after the operation. Food intake and bodyweight were not significantly different between the SG and SS groups. FBG: one week after operation, the SG group was significantly smaller than the SS group. The SS group was significantly lower than the SG group at 12 weeks after operation, and the SS group was significantly lower than the SG group at 14 weeks after operation. The transcription levels of c-kit mRNA and SCF mRNA in jejunum and ileum tissues are significantly different: the transcription levels of c-kit mRNA and SCF mRNA in the SG group are higher than those in the SS group, jejunum and ileum in the SG group. The number of cell 1 was significantly greater than that of the SS group. Sleeve gastrectomy can improve the regulation of the glucose metabolism in diabetic rats. The increase in small bowel motility may be related to the increase in ICC cells, intestinal cells, and GLP after gastric sleeve resection. The increase is in -1R and faster insoluble CHM in bowel motility. It has better contact with cell 1 and GLP-1R and stimulates cell 1 to secrete GLP-1.

This article provides the review of the medical use of pH- and temperature-sensitive polymer hydrogels. Such polymers are characterised by their thermal and pH sensitivity in aqueous solutions at the functioning temperature of living organisms and can react to the slightest changes in environmental conditions. Due to these properties, they are called stimuli-sensitive polymers. This response to an external stimulus occurs due to the amphiphilicity (diphilicity) of these (co)polymers. The term hydrogels includes several concepts of macrogels and microgels. Microgels, unlike macrogels, are polymer particles dispersed in a liquid and are nano- or micro-objects. The review presents studies reflecting the main methods of obtainingsuch polymeric materials, including precipitation polymerisation, as the main, simplest, and most accessible method for mini-emulsion polymerisation, microfluidics, and layer-by-layer adsorption of polyelectrolytes. Such systems will undoubtedly be promising for use in biotechnology and medicine due to the fact that they are liquid-swollen particles capable of binding and carrying various low to high molecular weight substances. It is also important that slight heating and cooling or a slight change in the pH of the medium shifts the system from a homogeneous to a heterogeneous state and vice versa. This providesthe opportunity to use these polymers as a means of targeted drug delivery, thereby reducing the negative effect of toxic substances used for treatment on the entire body and directing the action to a specific point. In addition, such polymers can be used to create smart coatings of implanted materials, as well as an artificial matrix for cell and tissue regeneration, contributing to a significant increase in the survival rate and regeneration rate of cells and tissues. References 1. Gisser K. R. C., Geselbracht M. J., Cappellari A.,Hunsberger L., Ellis A. B., Perepezko J., et al. 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Biologic or synthetic grafts have had limited success in small vessel applications. Studies were initiated to assess the potential use of cryopreserved (CP) arteries as coronary artery bypass conduits. Sheep carotid arteries (internal diameter: 4 mm; length: 10 cm) were cryopreserved in a nutrient media containing 10% DMSO and were stored in a nitrogen vapor at -150°C. After thawing, histological, enzyme-histochemical and functional studies showed slight histological alterations, preservation of enzymal activities and an abolition of the contractile response. In a sheep model, arterial substitution of a 10 cm segment of carotid artery was realised by implantation of fresh autografts (n=4); fresh allografts (n=9) and CP allografts (n=9). After 3 months, all autografts were patent with slight histological alterations. Fresh and CP allografts showed similar modifications: patency rate was 7/9 in both groups. Intimal thickening with cell proliferation was seen in fresh (3/7) and CP (4/8) arteries; loss of smooth muscle medial cells was constant. Adventitia was always involved by a marked inflammatory reaction. One characteristic of CP allografts was the frequent presence of large dystrophic calcifications. In conclusion, morphologic and functional arterial changes occurred after freezing and thawing. In spite of vascular rejection, the patency rate of allografts after 3 months of implantation in arterial circulation remained high and does not seem influenced by cryopreservation.

Simulasi desain implan sendi pinggul bertujuan untuk menganalisis total deformasi maksimum, tegangan prinsipal maksimum, dan tegangan geser maksimum dengan kombinasi variasi waktu, gerakan, dan pembebanan. Simulasi memungkinkan suatu objek diujicobakan secara visual sebelum diproduksi sebagai purwarupa. Fungsi dari simulasi adalah untuk meminimalisir terjadinya kegagalan dan untuk memangkas biaya produksi. Sebelum memulai simulasi suatu objek perlu dibuatkan desain. Desain dalam penelitian ini menggunakan software CAD yakni Inventor 2014, sedangkan analisis metode elemen hingga dalam desain implan memanfaatkan simulasi ANSYS 18.1. Analisis metode elemen hingga didasarkan pada aktivitas berjalan, melompat, dan menuruni tangga selama kurun waktu 0 detik hingga 4,5 detik. Hasil simulasi menunjukkan bahwa desain implan sendi pinggul menghasilkan 4079 nodal, 2157 Elemen, dan total deformasi maksimum sebesar 0,097 mm (berjalan), 0,2 mm (melompat), dan 0,11 mm (menuruni tangga). Tegangan prinsipal maksimum adalah 32 MPa (berjalan), 66,96 MPa (melompat), dan 73,93 MPa (menuruni tangga). Tegangan geser maksimum adalah 19,74 MPa (berjalan), 41,28 MPa (melompat), dan 45,58 MPa (menuruni tangga).Kata kunci: simulasi; implan sendi pinggul; metode elemen hingga; Mg Alloy.The simulation of the hip joint implant design aims to analyze maximum total deformation, maximum principal stress, and maximum shear stress with a combination of variations in time, motion and loading. Simulation allows an object to be tested visually before being produced as a prototype. Function of simulation is to minimize the occurrence of failures and to cut production costs. Before starting to simulate an object a design needs to be made. The design in this study used CAD software, namely Inventor 2014, while the finite element method analysis in implant design used ANSYS 18.1 simulation. Finite element method analysis is based on walking, jumping and descending stairs over a period of 0 seconds to 4.5 seconds. Simulation results show that hip joint implant design produces 4079 nodals, 2157 elements, and a maximum total deformation of 0.097 mm (walking), 0.2 mm (jumping), and 0.11 mm (descending stairs). Maximum principal stresses are 32 MPa (walking), 66.96 MPa (jumping), and 73.93 MPa (descending stairs). Maximum shear stresses are 19.74 MPa (walking), 41.28 MPa (jumping), and 45.58 MPa (descending stairs).Keywords : Simulation; Hip Joint Implants; Finite Element Method; Mg AlloyDAFTAR RUJUKANAhmed, A., Hameed, P., Shaikh, F., Hussain, Z., Hussain, N., & Aslam, M. (2017). Simulation tools application for arti fi cial lighting in buildings. Renewable and Sustainable Energy Reviews, August, 0–1. https://doi.org/10.1016/j.rser.2017.10.035Boyd, D. D. (2016). General aviation accidents related to exceedance of airplane weight/center of gravity limits. Accident Analysis and Prevention, 91, 19–23. https://doi.org/10.1016/j.aap.2016.02.019Conlisk, N., Howie, C. R., & Pankaj, P. (2017). Computational modelling of motion at the bone–implant interface after total knee arthroplasty: The role of implant design and surgical fit. The Knee, 24(5), 994–1005. https://doi.org/10.1016/j.knee.2017.07.003Constantinou, M., Loureiro, A., Carty, C., Mills, P., & Barrett, R. (2017). Hip joint mechanics during walking in individuals with mild-to-moderate hip osteoarthritis. Gait and Posture, 53, 162–167. https://doi.org/10.1016/j.gaitpost.2017.01.017Gu, X., Shiflet, G. J., Guo, F. Q., & Poon, S. J. (2005). Mg–Ca–Zn Bulk Metallic Glasses with High Strength and Significant Ductility. Journal of Materials Research, 20(08), 1935–1938. https://doi.org/10.1557/JMR.2005.0245Guo, W., Cui, W., Shi, Y., Liu, J., & Song, B. (2016). Function failure and failure boundary analysis for an aircraft lock mechanism. Engineering Failure Analysis, 70, 428–442. https://doi.org/10.1016/j.engfailanal.2016.10.003Hagihara, K., Shakudo, S., Fujii, K., & Nakano, T. (2014). Degradation behavior of Ca – Mg – Zn intermetallic compounds for use as biodegradable implant materials. Materials Science & Engineering C, 44, 285–292. https://doi.org/10.1016/j.msec.2014.08.037Hutař, P., Poduška, J., Šmíd, M., Kuběna, I., Chlupová, A., Náhlík, L., Polák, J., & Kruml, T. (2017). Short fatigue crack behaviour under low cycle fatigue regime. International Journal of Fatigue, 103, 207–215. https://doi.org/10.1016/j.ijfatigue.2017.06.002Kiani Khouzani, M., Bahrami, A., & Eslami, A. (2018). Metallurgical aspects of failure in a broken femoral HIP prosthesis. Engineering Failure Analysis, 90(November 2017), 168–178. https://doi.org/10.1016/j.engfailanal.2018.03.018Lestari, F. P., Kartika, I., Sriyono, B., Puspiptek, K., Tangerang, S., & Banten, S. (2013). Pengaruh Waktu Milling Pada Paduan Mg-Ca-Zn-CaH 2 Untuk Aplikasi Implan.Liu, X., Guo, J., Bai, C., Sun, X., & Mou, R. (2015). Drop test and crash simulation of a civil airplane fuselage section. Chinese Journal of Aeronautics, 28(2), 447–456. https://doi.org/10.1016/j.cja.2015.01.007Longman, J., Veres, D., & Wennrich, V. (2018). Utilisation of XRF core scanning on peat and other highly organic sediments. Quaternary International, January, 0–1. https://doi.org/10.1016/j.quaint.2018.10.015Meischel, M., Hörmann, D., Draxler, J., Tschegg, E. K., Eichler, J., Prohaska, T., & Stanzl-Tschegg, S. E. (2017). Bone-implant degradation and mechanical response of bone surrounding Mg-alloy implants. Journal of the Mechanical Behavior of Biomedical Materials, 71, 307–313. https://doi.org/10.1016/j.jmbbm.2017.03.025Mutlu, I. (2018). Production and fluoride treatment of Mg-Ca-Zn-Co alloy foam for tissue engineering applications. Transactions of Nonferrous Metals Society of China (English Edition), 28(1), 1–8. https://doi.org/10.1016/S1003-6326(18)64644-8Riccio, A., Cristiano, R., Saputo, S., & Sellitto, A. (2018). Numerical methodologies for simulating bird-strike on composite wings. Composite Structures. https://doi.org/10.1016/j.compstruct.2018.03.018Saini, M. (2015). Implant biomaterials: A comprehensive review. World Journal of Clinical Cases, 3(1), 52. https://doi.org/10.12998/wjcc.v3.i1.52Šamec, B., Potrč, I., & Šraml, M. (2011). Low cycle fatigue of nodular cast iron used for railway brake discs. Engineering Failure Analysis, 18(6), 1424–1434. https://doi.org/10.1016/j.engfailanal.2011.04.002Saulacic, N., Bosshardt, D. D., Bornstein, M. M., Berner, S., & Buser, D. (2012). BONE Apposition To A Titanium-Zirconium Alloy Implant , As Compared To Two Other Titanium-Containing Implants. 273–288.Seguin, C., Blaquière, G., Loundou, A., Michelet, P., & Markarian, T. (2018). Unmanned aerial vehicles ( drones ) to prevent drowning ☆. Resuscitation, 127(January), 63–67. https://doi.org/10.1016/j.resuscitation.2018.04.005Shi, K. K., Cai, L. X., Chen, L., Wu, S. C., & Bao, C. (2014). Prediction of fatigue crack growth based on low cycle fatigue properties. International Journal of Fatigue, 61, 220–225. https://doi.org/10.1016/j.ijfatigue.2013.11.007Yatim, F. (2006). Penyakit Tulang dan Persendian (Arthristis atau Arthralgia). Pustaka Populer Obor.Zameer, S., & Haneef, M. (2015). Fatigue Life Estimation of Artificial Hip Joint Model Using Finite Element Method. Materials Today: Proceedings, 2(4–5), 2137–2145. https://doi.org/10.1016/j.matpr.2015.07.220Zander, D., & Zumdick, N. A. (2015). Influence of Ca and Zn on the microstructure and corrosion of biodegradable Mg-Ca-Zn alloys. Corrosion Science, 93(January), 222–233. https://doi.org/10.1016/j.corsci.2015.01.027

Radioactive elements such as iodine (e.g., 129I or 131I isotopes), which is discharged into the environment as a result of nuclear detonations and reactor accidents, has a negative impact on human health and the ecosystem [1-3]. Radioactive iodine can be discharged as a direct aqueous pollutant when nuclear fission reactors are cooled with water. Both the Chernobyl and Fukushima nuclear disasters resulted in huge amounts of radioactive iodine being released into the atmosphere (the majority of which was subsequently deposited on the soil, where it leached into water), as well as directly into water bodies [1-3]. Medical is a secondary source of radioactive iodine, as radioactive isotopes are commonly employed for diagnostic and therapeutic purposes. Also, seawater is a natural source of iodine, due to that, the iodine can move into drinking water aquifers, causing increased iodine levels. The removal of radioactive elements such as iodine have been the target of numerus studies using different adsorption methods. However, most of the research to far has concentrated on the adsorption of iodine gas or iodine from organic solvents. There have been few cases of iodine removal from water at room temperature [1-3]. Nanotechnology have been widely used in many research projects related to environmental issues such as suitable energy and remediations [4-21]. The major goal of this research is to synthesize and investigate different nanoporous silicates with different pore size based on synthetic and naturally occurring materials such as zeolites modified with silver to remove iodine from water with high selectivity. The resultant materials are thoroughly investigated, via numerous characterization techniques such as XRD, XRF, SEM/EDX, TEM, and BET surface area measurements. The adsorption study shows a great promises for the resultant composites. For example, iodine was removed from aqueous solutions using synthetic zeolite-13X-Ag (Ag-13X) nanocomposites. The Ag-13X composites results revealed a high removal efficiency (up to 84% removal of iodine from aqueous media) compared to 13X that remove only 22% of iodine under the same adsorption conditions. References Steinhauser, A. Brandl , T.E. Johnson, 470–47, 800-817 (2014) Zhao, X., Han, X., Li, Z., Huang, H., Liu, D., Zhong, C., Appl. Surf. Sci. 351, 760–764 (2015). Tauanov, V.J. Inglezakis, Science of The Total Environment, 682, 259-270 (2019.). W.Chapman, P.J Chupas., T.M Nenoff. J. Am. Chem. Soc., 132, 8897–8899 (2010). ME Mahmoud, SS Haggag, MA Rafea, TM Abdel-Fattah, Polyhedron 28 (16), 3407-3414 (2009) C Huff, JM Long, A Aboulatta, A Heyman, TM Abdel-Fattah, ECS Journal of Solid State Science and Technology 6 (10), M115 (2017) A Wixtrom, J Buhler, T Abdel-Fattah, Journal of Chemical Education 91 (8), 1232-1235 (2014) ME Mahmoud, SS Haggag, TM Abdel-Fattah, Polyhedron 26 (14), 3956-3962 (2007) C Huff, T Dushatinski, A Barzanji, N Abdel-Fattah, K Barzanji, ECS Journal of Solid State Science and Technology 6 (5), M69 (2017) TM Abdel-Fattah, B Bishop, Journal of Environmental Science and Health, Part A 39 (11-12), 2855-2866 (2014) TM Abdel-Fattah, ME Mahmoud, MM Osmam, SB Ahmed, Journal of Environmental Science and health, part A 49 (9), 1064-1076 (2014) ME Mahmoud, TM Abdel-Fattah, MM Osman, SB Ahmed, Journal of Environmental Science and Health, Part A 47 (1), 130-141 (2012) C Huff, E Biehler, Q Quach, JM Long, TM Abdel-Fattah, Colloids and Surfaces A: Physicochemical and Engineering Aspects 610 (5), 125734 (2021) M Abdel-Fattah, A Wixtrom, K Zhang, W Cao, H Baumgart, ECS Journal of Solid State Science and Technology 3 (10), M61 (2014) M. Abdel-Fattah, M.E Mahmoud, M. M. Osmam, S.B. Ahmed, Journal of Environmental Science and health part A, 49, 1064-1076 (2014) C Huff, T Dushatinski, TM Abdel-Fattah, International Journal of Hydrogen Energy 42 (30), 18985-18990 (2017) C Huff, JM Long, A Heyman, TM Abdel-Fattah, ACS Applied Energy Materials 1 (9), 4635-4640 (2018) A Mahapatro, TD Matos Negrón, C Bonner, TM Abdel-Fattah, Journal of Biomaterials and Tissue Engineering 3 (2), 196-204 (2013) T Dushatinski, C Huff, TM Abdel-Fattah, Applied Surface Science 385, 282-288 (2016) ME Mahmoud, MM Osman, SB Ahmed, TM Abdel-Fattah, Chemical engineering journal 175, 84-94 (2011) TM Abdel-Fattah, ME Mahmoud, Chemical engineering journal 172 (1), 177-183 (2011) R Bhure, TM Abdel-Fattah, C Bonner, JC Hall, A Mahapatro, Journal of biomedical nanotechnology 6 (2), 117-128 (2010) TM Abdel-Fattah, D Loftis, A Mahapatro, Journal of biomedical nanotechnology 7 (6), 794-800 (2011) OH Elsayed-Ali, T Abdel-Fattah, HE Elsayed-Ali, Journal of hazardous materials 185 (2-3), 1550-1557 (2011) R Bhure, A Mahapatro, C Bonner, TM Abdel-Fattah, Materials Science and Engineering: C 33 (4), 2050-2058 (2013) BE Bishop, BA Savitzky, T Abdel-Fattah, Ecotoxicology and Environmental Safety 73 (4), 565-571 (2010)

In this study, a tubular hybrid vascular tissue composed of vascular cells and collagen was implanted as a venous substitute, and its remodeling process was histologically investigated. First, a hybrid medial tissue was prepared by pouring a cold mixed solution of canine jugular smooth muscle cells (SMCs) and Type I collagen into a tubular glass mold and subsequent incubation at 37°C. Culture in medium for 10 days produced a dense tubular tissue. Seeding of jugular endothelial cells (ECs) onto the luminal surface of the tissue produced a hybrid vascular tissue with a hierarchical structure. These vascular tissues (inner diameter, 7 mm; length, 3 cm; wall thickness, 1 mm; n = 14) were implanted autologously in the canine posterior vena cava wrapped in Dacron mesh for up to 24 wk. Nine of 14 grafts were patent throughout implantation. In patent grafts, monolayered ECs were oriented in the direction of blood flow at 1 wk. Circumferentially oriented SMCs accumulated at the subendothelial layer and ingrown fibroblasts were sparsely distributed throughout the wall at 12 wk. Contractile phenotype of SMCs was evident at 24 wk. Collagen fibrils, which were sparsely distributed at an early period of implantation, gradually assembled to form fibrous meshes at 24 wk. Sheet-like elastic lamellae were also observed at this time. Marked wall thinning was observed at 12 and 24 wk. The resultant tissues became highly dense. The specific gravity of tissues increased with time, and reached those of natural vessels at 24 wk. Tissue remodeling progressed in a time-dependent manner and appeared to be almost complete within 6 mo of implantation.

The aim of the present study was to evaluate the protective effects of linseed oil on lipid peroxidation, antioxidative capacity, as well as serum glucose, total cholesterol (TC), LDL-C and TG levels, in cafeteria-diet-fed dams during gestation and lactation, and in their offspring throughout adulthood. Food and energy intakes were also evaluated. The cafeteria diet led to higher energy intake, body weight, hyperglycemia and hyperlipidemia (higher TC, LDL-C and TG) in dams’ rats and their pups. Plasma vitamin C, Erythrocyte GSH levels and catalase activity were lower, whereas plasma diene conjugates (DC), Malondialdehyde and protein carbonyl levels (PC) in plasma and erythrocytes were higher in cafeteria-diet-fed mothers and their pups compared to controls. Supplement of linseed oil significantly enhanced plasma antioxidant defense capacities, as evaluated by the marked increase in the levels of plasma vitamin C and erythrocyte GSH as well as the activities of CAT and the significant reduction in lipid peroxidation (lower DC and MDA) and PC in CAFL-diet-fed mothers and their offspring. Simultaneous intake of linseed oil also reduced body weight, plasma TG, TC and LDL-C contents in obese pregnant and lactating dams and their pups at day 30 and day 90. The flaxseed oil supplementation may prevent lipid peroxidation and metabolic disorders which might be helpful in preventing obesity complications in mothers during pregnancy and lactation and in their offspring. REFERENCES:Adeoye, O., Olawumi, J., Opeyemi, A., & Christiania, O. (2016). Review on the role ofglutathione on oxidative stress and infertility. JBRA Assisted Reproduction, 22(1), 61-66.Aebi, H. (1974). Catalase. 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Healthcare education and practice has significantly been impacted by COVID-19. This includes the challenge on pedagogical approaches that highlight the potential of technology to facilitate innovative new approaches in response to social distancing, lockdowns, remote learning and improving the patient experience and positive outcomes. Many of these innovative approaches are not fundamentally new but are now seeing relevance beyond early adopters to mainstream implementation. This presentation draws upon collaborations with educational researchers and technologists that have explored the integration of technology into healthcare education and practice. COVID-19 Adversity to Opportunity Many healthcare programmes required reenvisaging teaching and learning approaches in response to COVID-19 restrictions. This had a particular impact on the development of interpersonal and practical knowledge and skills essential for healthcare graduates. The limited access to on-campus learning provided an opportunity for both institutional and individual evaluation of pedagogical practices. The affordances of traditional, didactic, and “hands-on” skills were compared with those that could be facilitated using online asynchronous/ synchronous strategies. A particular concern was the development of the interpersonal and practical skills required in safe and effective healthcare practice. Alongside easing of restrictions, these skills were adapted using online demonstrations within the limits of socially distanced “bubbles”, telehealth and limited clinical placements. Reconsideration of summative assessments was also required- with the introduction online synchronous and asynchronous verbal assessments, and asynchronous submissions of practical skills online (Cochrane et al., 2021; Narayan et al., 2021). In the prospect of COVID-19 restrictions continuing to lift, it is envisioned that most of the reenvisaged pedagogical approaches to healthcare education will persist, without compromising student critical thinking or practical skills. Interprofessional Collaboration This presentation will highlight the importance of interprofessional collaboration in healthcare curriculum design using a Design-Based-Research methodology (Chen et al., 2020; Kartoğlu et al., 2020) to facilitate authentic learning and develop self-determined learning capabilities for healthcare professionals. DBR- Design Principles in response to COVID Transferable design principles will be introduced for enhancing healthcare education that will improve practice in a COVID19 world, particularly drawing from eight healthcare projects including: STUDIO602 – enhancing clinical practice with mobile technologies (Cochrane & Sinfield, 2021), developing a virtual reality handover experience for healthcare students (Cochrane et al., 2018), using immersive reality to develop critical thinking in clinical health education (Stretton et al., 2018), enhancing first responder clinical simulation education using immersive reality and biometrics (Cochrane et al., 2020), designing authentic learning for graduate entry nursing students (Macdiarmid et al., 2021), designing public and environmental health education (Kersey et al., 2018), Biomedical engineering (Lam et al., 2021), and physiology education (Fabris et al., 2019). References Chen, W., Sandars, J., & Reeves, T. C. (2020). Navigating complexity: The importance of design-based research for faculty development. Medical Teacher, 1-3. https://doi.org/10.1080/0142159X.2020.1774530 Cochrane, T., Aiello, S., Cook, S., Aguayo, C., & Wilkinson, N. (2020). MESH360: A framework for designing MMR enhanced Clinical Simulations [Journal]. Research in Learning Technology, 28(Mobile Mixed Reality - Themed Collection). https://doi.org/10.25304/rlt.v28.2357 Cochrane, T., Narayan, V., Aiello, S., Birt, J., Cowie, N., Cowling, M., Deneen, C., Goldacre, P., Alizadeh, M., Sinfield, D., Stretton, T., & Worthington, T. (2021, 29th November- 1st December 2021). Post Pandemic Socially Constructed Blended Synchronous Learning: Vignettes from the Mobile Learning SIG. ASCILITE 2021: 38th International Conference on Innovation, Practice and Research in the Use of Educational Technologies in Tertiary Education, University of New England (UNE), Armidale, Australia. Cochrane, T., & Sinfield, D. (2021). STUDIO602: A model for designing real world collaborations between Higher education and Industry. In K. MacCallum & D. Parsons (Eds.), Industry Practices, Processes and Techniques Adopted in Education - Supporting innovative teaching and learning practice (Vol. In preparation). Springer. http://davidparsons.ac.nz/industry-in-ed/ Cochrane, T., Stretton, T., Aiello, S., Britnell, S., Cook, S., & Narayan, V. (2018). Authentic Interprofessional Health Education Scenarios using Mobile VR [Journal]. Research in Learning Technology, 26, 2130. https://doi.org/10.25304/rlt.v26.2130 Fabris, C. P., Rathner, J. A., Fong, A. Y., & Sevigny, C. P. (2019). Virtual Reality in Higher Education. International Journal of Innovation in Science and Mathematics Education (formerly CAL-laborate International), 27(8). Kartoğlu, Ü., Siagian, R. C., & Reeves, T. C. (2020). Creating a "Good Clinical Practices Inspection" Authentic Online Learning Environment through Educational Design Research. TechTrends : for leaders in education & training, 1-12. https://doi.org/10.1007/s11528-020-00509-0 Kersey, K., Lees, A., Conn, C., Cochrane, T., Narayan, V., & Williams, M. (2018). “Context matters”: The challenges and opportunities of designing tertiary public and environmental health education in South Auckland. Pacific Health, 1(1), 1-12. https://doi.org/https://doi.org/10.24135/pacifichealth.v1i1.8 Lam, L., Cochrane, T., Rajagopal, V., Davey, K., & John, S. (2021). Enhancing student learning through trans-disciplinary project-based assessment in bioengineering. Pacific Journal of Technology Enhanced Learning, 3(1), 4-5. https://doi.org/10.24135/pjtel.v3i1.80 Macdiarmid, R., Winnington, R., Cochrane, T., & Merrick, E. (2021). Using educational design research to develop authentic learning for Graduate Entry Nursing students in New Zealand. Nurse Education in Practice, 102965. https://doi.org/10.1016/j.nepr.2021.102965 Narayan, V., Cochrane, T., Aiello, S., Birt, J., Cowie, N., Cowling, M., Deneen, C., Goldacre, P., Alizadeh, M., Sinfield, D., Stretton, T., & Worthington, T. (2021, 29 November - 1 December). Mobile learning and socially constructed blended learning through the lens of Activity Theory. ASCILITE 2021: 38th International Conference on Innovation, Practice and Research in the Use of Educational Technologies in Tertiary Education, University of New England (UNE), Armidale, Australia. Stretton, T., Cochrane, T., & Narayan, V. (2018). Exploring Mobile Mixed Reality in Healthcare Higher Education: A Systematic Review [Journal]. Research in Learning Technology, 26, 2131. https://doi.org/10.25304/rlt.v26.2131

Objective: To study the effect of estrogen on lungs of adult male mice by assessing and comparing the following histological parameters including bronchiolar smooth muscle size and peri-bronchial lymphocytic Infiltration. Study Design: Randomized control trial Place and Duration of Study: National institute of Health sciences, Islamabad from 1st October 2018 to 31st July 2019. Methodology: Ninety BALB/c mice divided into 2 groups (n=30 per group) were enrolled. The control (male) group received only distilled water, while the interventional groups received pills (estradiol valerate) mixed in distal water according to body weight of the mice for (60) day. Results: 10% of the total mice had nil, 60% had mild, 30% of the total showed moderate while no severe peri-bronchiolar lymphocytic infiltration in response to estrogen. The results also showed that estrogen produced marked hyperplasia of bronchial smooth muscle cells. Conclusion: Estrogen is the sexual hormones which modulate inflammatory processes in the lungs producing pulmonary inflammatory responses leading to asthma and causes hyperplasia of the bronchiolar smooth muscles. Keywords: Estrogen, Asthma, Bronchiolar smooth muscle, Peri-bronchial lymphocytic infiltration, BALB/c mice

Abstract Diabetic foot syndrome is a secondary disease of diabetes mellitus that can lead to amputations, reduced mobility and in some cases death if treatment is delayed. The objective of this study was to collect user requirements for the development of a shoe insole accompanied by a medical app for continuous pressure measurement, applying a usercentered Design Thinking process.We conducted a systematic competitor analysis and performed 10 context-interviews with both health care professionals (HCPs) (n=7) and patients with diabetic foot syndrome (n=3) at the University Hospital Graz (Austria) and a medical supply store. Structured interview guides, tailored to user groups were used for qualitative interviews. Questionnaires on the acceptance of eHealth applications were designed for quantitative analysis (n=14 patients, n=7 HCPs). The competitor analysis identified 12 inshoe- systems for plantar pressure measurement for patients with diabetes. No current system on the EU market meets the requirements of the users interviewed in this study. Quantitative evaluation showed that 95% of future users would support the use of a medical app to monitor plantar pressure in patients with diabetic foot syndrome. Based on the identified user requirements, a first user interface was developed iteratively. Usability tests of the prototype resulted in a score of 71.7 on the System Usability Scale. The medical app will be further developed and tested in clinical studies.

A “super” bioactive antibacterial hydrogel, Icariin-β-CD-inclusion complex/Bacterial cellulose and an equally capable counterpart Icariin-Bacterial cellulose (ICBC) were successfully produced with excellent antioxidant properties. The highly porous hydrogels demonstrated very high fluid/liquid absorption capability and were functionally active as Fourier Transform Infrared Spectrometer (FTIR) test confirmed the existence of abundant hydroxyls (-OH stretching), carboxylic acids (-CH2/C-O stretching), Alkyne/nitrile (C≡C/C≡N stretching with triple bonds) and phenol (C-H/N-O symmetric stretching) functional groups. Scanning electron microscope (SEM) and X-ray diffraction (XRD) tests confirmed a successful β-CD-inclusion complexation with Icariin with a great potential for sustained and controlled drug release. In vitro drug release test results indicated a systemic and controlled release of the drug (Icariin) from the internal cavities of the β-CD inclusion complex incorporated inside the BC matrix with high Icariin (drug) release rates. Impressive inactivation rates against Gram-negative bacteria Escherichia coli ATCC 8099 and gram-positive bacteria Staphylococcus aureus ATCC 6538; >99.19% and >98.89% respectively were recorded, as the materials proved to be non-toxic on L929 cells in the in vitro cytotoxicity test results. The materials with promising versatile multipurpose administration of Icariin for wound dressing (as wound dressers), can also be executed as implants for tissue regeneration, as well as face-mask for cosmetic purposes.

Dendrons fitted with three oligo(ethylene glycol) (OEG) chains, one of which contains a fluorinated or hydrogenated end group and bears a bisphosphonate polar head (C n X2 n +1OEG8Den, X = F or H; n = 2 or 4), were synthesized and grafted on the surface of iron oxide nanoparticles (IONPs) for microbubble-mediated imaging and therapeutic purposes. The size and stability of the dendronized IONPs (IONP@C n X2 n +1OEG8Den) in aqueous dispersions were monitored by dynamic light scattering. The investigation of the spontaneous adsorption of IONP@C n X2 n +1OEG8Den at the interface between air or air saturated with perfluorohexane and an aqueous phase establishes that exposure to the fluorocarbon gas markedly increases the rate of adsorption of the dendronized IONPs to the gas/water interface and decreases the equilibrium interfacial tension. This suggests that fluorous interactions are at play between the supernatant fluorocarbon gas and the fluorinated end groups of the dendrons. Furthermore, small perfluorohexane-stabilized microbubbles (MBs) with a dipalmitoylphosphatidylcholine (DPPC) shell that incorporates IONP@C n X2 n +1OEG8Den (DPPC/Fe molar ratio 28:1) were prepared and subsequently characterized using both optical microscopy and an acoustical method of size determination. The dendrons fitted with fluorinated end groups lead to smaller and more stable MBs than those fitted with hydrogenated groups. The most effective result is already obtained with C2F5, for which MBs of ≈1.0 μm in radius reach a half-life of ≈6.0 h. An atomic force microscopy investigation of spin-coated mixed films of DPPC/IONP@C2X5OEG8Den combinations (molar ratio 28:1) shows that the IONPs grafted with the fluorinated dendrons are located within the phospholipid film, while those grafted with the hydrocarbon dendrons are located at the surface of the phospholipid film.

Cu–N–C single-atom nanozymes were successfully designed via a one-pot solvothermal method and their excellent peroxidase-mimicking activity has been investigated and applied for H₂O₂ and glucose determination.

In cardiopulmonary resuscitation (CPR), in practice, the rescuer usually uses two hands to perform the action of chest compressions. During chest compressions action, the two arms of the rescuer actually constitute a parallel mechanism. Inspired by this performance, this paper presents a novel structure of parallel manipulators from Delta robot family for chest compressions in rescuing a patient. Also, two new control methodologies are applied to track the desired trajectory. Based on one supervisory approach and another one based upon adaptive neuro-fuzzy inference system (ANFIS) approach. Inverse dynamic modeling is performed based on principle of virtual work and the results are verified using MSC.Adams© software. The proportional derivative (PD) controllers of computed torque (C-T) method usually need manual retuning to make a successful task, particularly in the presence of disturbance. In the present paper, we study and compare the feasibility of applying supervisory controller and ANFIS instead of conventional controller used in C-T method to cope with the above mentioned problem. Several computer simulations imply that the proposed method is encouraging compared with C-T method implemented with conventional controller.

Abstract Encapsulation is essential for mechanically flexible and electrically active implants as it protects them from the harsh environment inside the body. Since the performance and longevity of implants depends on the quality of encapsulation, research continues into new and better encapsulation strategies. Chemical vapour deposition can be used to deposit Parylene-C, a biocompatible polymer, at room temperature. Plasma enhanced atomic layer deposition can be used to deposit ultra-thin, conformal, and hermetic metal oxide layers. These strategies are both used for the encapsulation of implants. In this work, these two methods are combined to create a polymer/metal oxide hybrid system in which a layer system of metal oxides is sandwiched between two Parylene- C layers. DC leakage-current measurement and elevated temperature are used to qualify the barrier property of the layer-system. After more than one year, the barrier properties still show no significant decrease.

Constant force component is very useful in medical device, such as forceps with constant force, which may prevent soft tissues from injures due to overloading. This paper studied the optimization procedure in constant force component for superelastic shape memory alloy, and tried to find the rule of obtaining constant force within a relatively large deformation range for superelastic C-shaped shape memory alloy sheet. The optimization concept of combing finite element analysis in ANSYS with genetic algorithm in MATLAB was presented for designing constant force component using superelastic SMA. The computational optimization and experimental results of the C-shaped shape memory alloy sheet showed that the proposed optimization method was potential for superelastic shape memory alloy. The optimization results were consistent with the experimental results. It was demonstrated that constant force could be obtained within a relatively large deformation range by varying the initial shape of the superelastic SMA component.

Biobanking is one of the most important elements of the modern infrastructure for biomedical research. Organization of a biobank on the basis of the N. P. Bochkov Medical Genetics Research Center provides a centralized infrastructure for preparing biomaterial for research. Biobank has the format of a research equipment sharing center and works with two types of unique biomaterials from patients with genetic diseases: blood/blood components and vital cells of various tissue origin. The storage facility of the Biobank is equipped with low-temperature (-80° C) and cryostorage (-196° C) systems. Identification and search of samples is carried out using a bar-coding system and is implemented through the information interface of the biobank, which is integrated into the general database of patients at the Medical Genetics Research Center. Information on biomaterial samples is presented in periodically updated catalogs on the page of equipment sharing center “Biobank”. Biobank collection is available to internal and external users.

A linear optimization model was formulated using a semi-experimental protocol to estimate the forces in the spinal elements of a lumbar motion segment subjected to an extension or lateral bending moment with and without a 120 N compressive preload. A morphometer was used to acquire the three-dimensional locations of the disk center, facet centers and ligament origin and insertion sites with the specimen in a “neutral” position. The relative motion of the superior vertebra, under the loading conditions tested, was monitored using a Selspot II® system. These data allowed the formulation of the static equilibrium equations for the superior vertebra at each of the loading conditions mentioned above. A linear optimization technique was used, along with a suitable cost function, to find an optimum solution for the set of equations and imposed constraints. Results showed that for 6.9 Nm of extension moment, each facet carried a load of 52 N, with the disk carrying an axial tensile load of 104 N. At the 6.9 Nm extension moment coupled with 120 N preload, each facet carried a load of 77.2 N and the disk an axial tensile load of 37 N. In right lateral bending, with and without preload, the load was distributed among the right facet, the disk, the left ligamentum flavum and the left capsular ligament. At the 6.9 Nm load step without preload the right facet carried an axial load of 127.01 N with the disk carrying an axial compressive load of 7.8 N. Ligament forces for this step for the left ligamentum flavum and capsular ligament, respectively, were 61.03 N and 65.14 N. The addition of 120 N of preload reduced the load on the right facet to 83.5 N. The compressive load in the disk increased to 107.5 N. The corresponding ligament forces were 43.2 N (left ligamentum flavum) and 50.7 N (left capsular ligament).

Recent studies show that during slow freezing of biological cells, the cells may be also injured by not only chemical damage but also mechanical damage induced by ice crystal compression. A new experimental procedure is developed to quantify cell destruction by deformation with two parallel surfaces. The viability of cells (prostatic carcinoma cells, 17.5 μm in mean diameter) is measured as a function of gap size ranging from 3.5 μm to 16.2 μm at 0°C, 23°C and 37°C. The viability at a smaller gap size is significantly lower at 37°C than at 23°C, while the difference between 0°C and 23°C is much smaller. This suggests that deformation damage is related to the deformation of the cytoskeleton rather than the mechanical properties of the lipid membrane.

Abstract We present a thermal processing device, equipped for local heating and welding of biomaterials, e.g. electrospun nonwovens or polymer films. Depending on the application and choice of materials, we aim to determine appropriate processing parameters for permanent and non-permanent welds. Due to the modular device structure, applicationspecific heating tools can be used. Process temperature up to 250 °C is feasible. In this concept study, a welding tool with interchangeable small-size heating tips of different geometric shapes were investigated regarding their suitability for welding of polycarbonate urethane based silicone elastomer nonwovens. Generated welds were examined by imaging techniques and tensile testing. Regardless of the tip shape, welds were generated withstanding forces up to 3.0 N and 4.3 N for tensile and shear loading, respectively.

We performed in vitro pressure-diameter and axial force-length experiments on nondiseased, passive bovine coronary arteries subjected to bath temperatures from 21 to 80° C for 90 s to 4 hr. Over the strain ranges studied, we found that: (a) vessel behavior remained the same over 20 min of testing at 21 to 55° C, (b) vessels stiffened multiaxially after 5 min of exposure to 60° C and continued to stiffen over 20 min of testing, (c) dramatic multiaxial vessel stiffening and shrinkage occurred after 90 s of exposure to 70 and 80° C, and (d) heat-induced changes at 70° C depended on the intraluminal pressure during heating. Thus, passive bovine coronary arteries exhibit a complex thermomechanical behavior that depends on the temperature, duration of thermal exposure, and the mechanical loads applied during heating.

The rate at which equine and macaque ovarian tissue sections are first cooled from +25°Cto+4°C has a significant effect on the measured water transport when the tissues are subsequently frozen in 0.85M solutions of glycerol, dimethylsulfoxide (DMSO), or ethylene glycol (EG). To determine whether the response of ovarian tissues is altered if they are suspended in mixtures of cryoprotective agents (CPAs), rather than in solutions of a single CPA, we have now measured the subzero water transport from ovarian tissues that were suspended in mixtures of DMSO and EG. Sections of freshly collected equine and macaque ovaries were suspended either in a mixture of 0.9M EG plus 0.7M DMSO (equivalent to a mixture of ∼5%v∕v of EG and DMSO) or in a 1.6M solution of only DMSO or only EG. The tissue sections were cooled from +25°Cto+4°C and then frozen to subzero temperatures at 5°C∕min. As the tissues were being frozen, a shape-independent differential scanning calorimeter technique was used to measure water loss from the tissues and, consequently, the best fit membrane permeability parameters (Lpg and ELp) of ovarian tissues during freezing. In the mixture of DMSO+EG, the respective values of Lpg and ELp for equine tissue first cooled at 40°C∕min between +25°C and +4°C before being frozen were 0.15μm∕minatm and 7.6kcal∕mole. The corresponding Lpg and ELp values for equine tissue suspended in 1.6M DMSO were 0.12μm∕minatm and 27.2kcal∕mole; in 1.6M EG, the values were 0.06μm∕minatm and 21.9kcal∕mole, respectively. For macaque ovarian tissues suspended in the mixture of DMSO+EG, the respective values of Lpg and ELp were 0.26μm∕minatm and 26.2kcal∕mole. Similarly, the corresponding LLg and ELp values for macaque tissue suspended in 1.6M DMSO were 0.22μm∕minatm and 31.4kcal∕mole; in 1.6M EG, the values were 0.20μm∕minatm and 27.9kcal∕mole. The parameters for both equine and macaque tissue samples suspended in the DMSO+EG mixture and first cooled at 0.5°C∕min between +25°C and +4°C were very similar to the corresponding values for samples cooled at 40°C∕min. In contrast, the membrane parameters of equine and macaque samples first cooled at 0.5°C∕min in single-component solutions were significantly different from the corresponding values for samples cooled at 40°C∕min. These results show that the membrane properties of ovarian cells from two species are different, and that the membrane properties are significantly affected both by the solution in which the tissue is suspended and by the rate at which the tissue is cooled from +25°Cto+4°C before being frozen. These observations suggest that these variables ought to be considered in the derivation of methods to cryopreserve ovarian tissues.

The self-heated thermistor technique was used to measure the thermal conductivity and thermal diffusivity of biomaterials at low temperatures. Thermal standards were selected to calibrate the system at temperatures from −10°C to −70°C. The thermal probes were constructed with a convection barrier which eliminates convection inside liquid samples of low viscosity, without affecting the conductivity and diffusivity results. Using this technique, the thermal conductivity and diffusivity of two organ perfusates (HP5 and HP5 + 2M glycerol), one kidney phantom (a low ionic strength gel), as well as rabbit kidney cortex have been measured from −10°C to −70°C.

Drug resistance is a huge challenge during the management of diseases. MicroRNA (miRNA) dys-regulation is known to contribute to tumor progression. Herein we aimed to explore miR-1254’s role in drug resistance in lung cancer. In the present study, we used Pabolizumab to treat drug-resistant and non-drug resistant lung cancer cells followed by analysis of miR-1254 expression by RT-qPCR, epithelial-mesenchymal transition (EMT) related protein and c-Myc expression by western blot, E-cadherin and N-cadherin level by immunofluorescence. Additionally, mouse model of lung cancer was treated with miR-1254 mimic and/or Pabolizumab to assess miR-1254’s role in lung cancer in vivo. Drug-resistant lung cancer cells exhibited significantly increased viability upon treatment with Pabolizumab with decreased miR-1254 expression. Besides, Pabolizumab upregulated E-caderin and downregulated N-cadherin. Importantly, miR-1254 bound to c-Myc in cancer cells. In the presence of miR-1254 mimic or siRNA (si)-c-Myc, the chemosensitivity of lung cancer cells was increased whereas miR-1254 inhibitor augmented cell resistance to Pabolizumab. Furthermore, the chemosensitivity induced by c-Myc could be depleted by miR-1254 inhibitor. Combined treatment of miR-1254 mimic and Pabolizumab significantly decreased tumor weight and volume, and reduced c-Myc level. In conclusion, miR-1254 might suppress EMT by inhibiting c-Myc expression in lung cancer and decrease drug resistance.

The bacterium Xanthomonas campestris, which synthesizes the commercially important polysaccharide xanthan, was grown aseptically in 1.2 L fermenters using semicontinuous cell culture technique (d′ = 0.0035 h−1). The effects of carbon-substrate concentration on xanthan production were investigated at three initial glucose concentrations (G0 = 15, 20, 25 g/L). Cell biomass synthesis was nitrogen-limited by use of a chemically defined medium that contained NH3-N as the sole nitrogen source at a concentration where it was exhausted before glucose. A linear relationship between biomass synthesis and NH3-N depletion was observed. This relationship remained valid only until NH3-N exhaustion, after which biomass concentration slowly rose another 20 percent before declining. Another linear relationship was found between xanthan synthesis and glucose uptake. This relationship was unaffected by the disappearance of NH3-N and held through glucose exhaustion. The quasi-stoichiometric yield coefficients obtained for each linear relationship were not affected by G0. Biomass synthesis kinetics showed no variation with G0 before NH3-N exhaustion; afterwards, cell biomass decline was delayed by increasing G0. Xanthan synthesis kinetics displayed no detectable response to depletion of NH3-N and plateauing of biomass concentration; however, there was a marked slow down in the net rate of xanthan synthesis and a drop in xanthan yield after cell biomass decline became noticeable.

Additive manufacturing's attributes include print customization, low per-unit cost for small- to mid-batch production, seamless interfacing with mainstream medical 3D imaging techniques, and feasibility to create free-form objects in materials that are biocompatible and biodegradable. Consequently, additive manufacturing is apposite for a wide range of biomedical applications including custom biocompatible implants that mimic the mechanical response of bone, biodegradable scaffolds with engineered degradation rate, medical surgical tools, and biomedical instrumentation. This review surveys the materials, 3D printing methods and technologies, and biomedical applications of metal 3D printing, providing a historical perspective while focusing on the state of the art. It then identifies a number of exciting directions of future growth: ( a) the improvement of mainstream additive manufacturing methods and associated feedstock; ( b) the exploration of mature, less utilized metal 3D printing techniques; ( c) the optimization of additively manufactured load-bearing structures via artificial intelligence; and ( d) the creation of monolithic, multimaterial, finely featured, multifunctional implants.