Relevant bibliographies by topics / Medicago sativa

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Academic literature on the topic 'Medicago sativa'

Author: Grafiati
Published: 4 June 2021
Last updated: 10 March 2023

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Journal articles on the topic "Medicago sativa"

1

Basch, Ethan, Catherine Ulbricht, Michelle Harrison, David Sollars, Michael Smith, Cathi Dennehy, and Philippe Szapary. "Alfalfa (Medicago sativa L.)." Journal Of Herbal Pharmacotherapy 3, no. 2 (March 1, 2003): 69–90. http://dx.doi.org/10.1300/j157v03n02_09.

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2

Eremenko, R. F., L. N. Maloshtan, and E. Yu Yatsenko. "Experimental study of hypoglycemic effect of medicago sativa leaves extract." Kazan medical journal 95, no. 4 (August 15, 2014): 557–61. http://dx.doi.org/10.17816/kmj1843.

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Aim. To study the hypoglycemic effect of the Medicago Sativa leaves extract in intact rats and in rats with a glucose load. Methods. At the first stage, hypoglycemic effect of Medicago Sativa leaves extract was determined in intact rats. 50 mg/kg of metformin and 18 mg/kg of herbal anti-diabetic «Arfazetin» tea were used as comparator drugs. At the second stage, hypoglycemic effect of Medicago Sativa leaves extract was determined in rats with glucose load. Blood samples for glucose analysis were taken before and in 15, 30, 60 and 120 minutes after loading. Blood glucose level was determined by glucose oxidase method. Results. Medicago Sativa leaves extract showed hypoglycemic effect both in intact animals and in animals with glucose load. 25 mg/kg of Medicago Sativa leaves extract decreased blood glucose level in intact animals at 4, 6 and 8 hours by 1.04, 1.14 and 1.11 times compared to the baseline level; the effect was comparable with herbal anti-diabetic «Arfazetin» tea and was inferior to metformin. A single 25 mg/kg dose of Medicago Sativa leaves extract showed hypoglycemic effect in animals with glucose load and significantly reduced blood glucose level in 15, 30, 60 and 120 minutes compared to control. Conclusion. The findings suggest that 25 mg/kg of Medicago Sativa leaves extract can be used as a herbal medication in the complex treatment of type II diabetes mellitus for its hypoglycemic properties.
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Milentyeva, Irina Sergeevna, Anastasiya Igorevna Dmitrieva, Lyudmila Konstantinovna Asyakina, and Yulia Vladimirovna Golubtsova. "Analysis of individual biologically active compounds from root crop extracts of purple alfalfa (medicago sativa) in vitro." LAPLAGE EM REVISTA 7, no. 2 (May 18, 2021): 564–74. http://dx.doi.org/10.24115/s2446-6220202172864p.564-574.

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The authors selected the parameters for obtaining root cultures in vitro Medicago sativa. The number of cell culture lines studied is 15. The duration of the growth cycle is less than 50 days. The number of processed explants is 75. The growth characteristics of root cultures of in vitro purple alfalfa (Medicago sativa) were studied. The growth index for the dry biomass of the in vitro root culture of alfalfa was 31. A qualitative and quantitative analysis of the composition of BAS in the biomass of the obtained root cultures of in vitro alfalfa (Medicago sativa) was carried out. Unique biologically active substances (not previously described for alfalfa) were identified.): apigenin, naringenin, myricetin, chrysoeriol, coumestrol, and scopoletin. Rational parameters of isolation of individual BAS from the extract of root cultures of in vitro alfalfa (Medicago sativa) were selected. The degree of extraction of individual BAS is 80%. Methods of purification of individual BAS Medicago sativa have been developed. The degree of purification of individual BAS is 95%.
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Blondon, François, Dominique Marie, Spencer Brown, and Adam Kondorosi. "Genome size and base composition in Medicago sativa and M. truncatula species." Genome 37, no. 2 (April 1, 1994): 264–70. http://dx.doi.org/10.1139/g94-037.

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The genome size (1C value) and base composition of 14 ecotypes of two species of tetraploid and diploid Medicago have been assessed by flow cytometry. These parameters vary both between and within species. The diploid annual Medicago truncatula Gaertn. had the smallest genome of the group studied (which also covered M. sativa L. subsp. sativa, M. sativa L. subsp. caerulea (Less. ex Ledeb.) Schmalh., M. sativa L. subsp. quasifalcata Sinsk., M. sativa L. subsp. × varia (Martyn) Arcangeli; however, its ecotypes revealed substantial intraspecific variation. The smallest M. truncatula genome observed was ecotype 108-1 with 1C = 0.49 pg and 38.1% GC and the largest was Jemalong with 1C = 0.57 pg and 38.6% GC. The degree of polysomaty in these Medicago was low, although in some tissues the frequency of cells with 4C nuclei reached 50%.Key words: Medicago, genome size, base composition, flow cytometry, symbiosis.
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Natelson, S. "Canavanine in alfalfa (Medicago sativa)." Experientia 41, no. 2 (February 1985): 257–59. http://dx.doi.org/10.1007/bf02002625.

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Chaudhary, Anjali, Shivam ., Neetu Sachan, and Phool Chandra. "A Comprehensive Review: Medicago sativa." International Journal of Pharmaceutical Sciences Review and Research 65, no. 1 (November 15, 2020): 194–200. http://dx.doi.org/10.47583/ijpsrr.2020.v65i01.029.

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7

Segovia-Lerma, A., R. G. Cantrell, J. M. Conway, and I. M. Ray. "AFLP-based assessment of genetic diversity among nine alfalfa germplasms using bulk DNA templates." Genome 46, no. 1 (February 1, 2003): 51–58. http://dx.doi.org/10.1139/g02-100.

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Improving commercial utilization of perennial Medicago collections requires developing approaches that can rapidly and accurately characterize genetic diversity among large numbers of populations. This study evaluated the potential of using amplified fragment length polymorphism (AFLP) DNA markers, in combination with DNA bulking over multiple genotypes, as a strategy for high-throughput characterization of genetic distances (D) among alfalfa (Medicago sativa L.) accessions. Bulked DNA templates from 30 genotypes within each of nine well-recognized germplasms (African, Chilean, Flemish, Indian, Ladak, Medicago sativa subsp. falcata, Medicago sativa subsp. varia, Peruvian, and Turkistan) were evaluated using 34 primer combinations. A total of 3754 fragments were identified, of which 1541 were polymorphic. The number of polymorphic fragments detected per primer combination ranged from 20 to 85. Pairwise D estimates among the nine germplasms ranged from 0.52 to 1.46 with M. sativa subsp. falcata being the most genetically dissimilar. Unweighted pair-group method arithmetic average (UPGMA) analysis of the marker data produced two main clusters, (i) M. sativa subsp. sativa and M. sativa subsp. varia, and (ii) M. sativa subsp. falcata. Cluster-analysis results and D estimates among the Chilean, Peruvian, Flemish, and M. sativa subsp. varia germplasms supported the hypothesis that Peruvian was more similar to original Spanish introductions into Central and South America than Chilean. Hierarchical arrangement of the nine germplasms was supported by their respective geographic, subspecific, and intersubspecific hybrid origins. Subsets of as few as seven highly informative primer pairs were identified that produced comparable D estimates and similar heirarchical arrangements compared with the complete dataset. The results indicate that use of primer-pair subsets for AFLP analysis of bulk DNA templates could serve as a high-throughput system for accurately characterizing genetic diversity among large numbers of alfalfa populations.Key words: Medicago sativa, DNA bulking, genetic distance.
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Sharma, Prashant, Sujit Das, and Tanmay Mohanta. "A Study on the Wound Healing Properties of Medicago sativa." Journal of Drug Delivery and Therapeutics 11, no. 4 (July 15, 2021): 132–35. http://dx.doi.org/10.22270/jddt.v11i4.4940.

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There are lots of herbal plants are using for various treatment, food remedy etc from the prehistoric time. India has taken a great part to garage lots of herbal plants. 3-12 years lifespan containing and cool climate growing Medicago sativa is one of them good rank holder herbal plant. After studying the chemical constituents of M. sativa, it contains vitamin C, vitamin K, genistein which have great wound healing activity as well as it can cure kidney pain, cough, sore muscle, asthma etc. But it cannot take those people who are suffering breast cancer, ovarian cancer etc. At last, after overall study, Medicago sativa has great reflection on human and animals. Keywords: Medicago sativa, wound healing, vitamin C, vitamin K, genistein, food remedy.
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R, Gomathi, Banu S, and Usha K. "PHYTOCHEMICAL ANALYSIS AND IN VITRO FREE RADICAL SCAVENGING ACTIVITIES OF MEDICAGO SATIVA SEEDS." Kongunadu Research Journal 2, no. 1 (June 30, 2015): 128–32. http://dx.doi.org/10.26524/krj82.

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Phytochemical analysis and in vitro free radical scavenging activities were analyzed in the various extracts of Medicago sativa seeds. The phytochemical analysis showed the presence of alkaloids, carbohydrates, flavonoids, glycosides, saponins, phytosterols, tannins, terpenoids and phenols. Among the various extracts, phytochemicals were extracted best in ethanol. Free radical scavenging activities such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, superoxide, 2,2'-azino-bis (3-ethylbenzothiazoline-6- sulphonic acid) (ABTS), ferrous ion chleating activity and non radicals such as hydrogen peroxide and nitric oxide were analyzed in the various extracts of Medicago sativa seeds and were compared with standard antioxidant ascorbic acid. All the extracts of Medicago sativa seeds scavenged the free radicals in a concentration dependent manner. The antioxidative activity of all the extracts was found to be more pronounced than that of the standard antioxidant ascorbic acid. Among the various extracts, the antioxidant activity was found to be more pronounced in ethanolic extract of Medicago sativa seeds
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Molor, Adiyasuren, Altantsetseg Khajidsuren, Uuganzaya Myagmarjav, and Enkhchimeg Vanjildorj. "COMPARATIVE ANALYSIS OF DROUGHT TOLERANCE OF MEDICAGO L. PLANTS UNDER STRESSED CONDITIONS." Mongolian Journal of Agricultural Sciences 19, no. 3 (January 10, 2017): 32–40. http://dx.doi.org/10.5564/mjas.v19i3.733.

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As a perennial forage crop, alfalfa (Medicago L.) can be cultivated in marginal lands and has a high yield and good quality of high-protein content. Alfalfa has deep vertical roots; this species is able to absorb even depth waters about 5 m depth and more. This advantage saves plant’s life in long term drought. The objective of this study was to compare the performance of drought stress during germination and growth stage of 2 species (Medicago sativa, Medicago falcata) and 2 varieties (Mongolian Medicago varia Marthz var.Burgaltai and Inner Mongolian Medicago varia Martyn var.Nutag Belcheer-2) in laboratory condition. In order to expose the drought stresses in plant by polyethylene glycol (PEG 4000) with 0 (control), -0.3, -0.5 and -1.0 MPa for 2 weeks respectively. The results represented that Medicago sativa had higher seed germination percentage than other species and varieties. Water uptake, dry matter index are observed in M.sativa higher than others.The highest chlorophyll content was in Medicago varia Marthz var.Burgaltai. The highest result of root, shoot length and weight were observed in Medicago sativa. In contrast, the significantly lowest result of morphological parameters was in Mediago varia Martyn var.Nutag Belcheer-2. M.sativa is highly tolerant to drought stress among species. M.varia Marth var.Burgaltai was moderately tolerant to drought stress among varieties.
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Dissertations / Theses on the topic "Medicago sativa"

1

Forsthoefel, Nancy Rose 1963. "Mitochondrial inheritance in Medicago sativa." Thesis, The University of Arizona, 1991. http://hdl.handle.net/10150/291585.

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Numerous studies show that plastid DNA is inherited biparentally in alfalfa (Medicago sativa L.). Mitochondrial DNA has been shown to be inherited in a uniparental-maternal fashion in a limited number of sexual crosses. This study investigated the inheritance of mitochondrial DNA in 54 alfalfa progenies from crosses between two cytoplasmic male sterile maternal plants and 54 paternal plants, representing each of the eight basic germplasm groups of M. sativa. Cloned mitochondrial DNA fragments from M. sativa were used as hybridization probes to identify polymorphisms in mitochondrial DNA for Eco RI restriction sites. Polymorphisms were analyzed for patterns of mitochondrial DNA inheritance. All progenies displayed the Eco RI mitochondrial DNA restriction pattern of the maternal parent, indicating that maternal mitochondrial inheritance predominates in alfalfa. The same progenies were also examined for their plastid DNA inheritance pattern. Plastid DNA displayed a mixed inheritance pattern, with both uniparental-maternal and uniparental-paternal inheritance. Variation observed between paternal parents for plastid transmission was absent for mitochondrial transmission.
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au, ycheng@murdoch edu, and Yvonne Cheng. "Plant Mechanisms Contributing to Acid Impairment of Nodulation of Medicago murex and Medicago sativa by Sinorhizobium medicae." Murdoch University, 2003. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20040504.151812.

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The widespread sowing of the perennial forage legume Medicago sativa to lower groundwater tables in south-western Australia is limited as many soils targeted for its use are too acidic (pHCaCl2 < 5) for consistent nodulation with Sinorhizobium spp. The annual medic M. murex grows and nodulates well in these acidic soils, but it cannot fill the niche of M. sativa in lowering groundwater tables. The differential ability of M. murex and M. sativa to nodulate in acid soils provided the opportunity to compare the nodulation responses between the two species and to identify the mechanisms contributing to the poor nodulation of M. sativa in soil of low pH. An initial glasshouse experiment compared the nodulation of M. murex cv. Zodiac and M. sativa cv. Aquarius with S. medicae strains WSM419 and CC169. Subsequent glasshouse and laboratory experiments used only the more acid-tolerant S. medicae strain WSM419. In the glasshouse in soil of pHCaCl2 4.3, the uppermost nodule on both M. murex and M. sativa formed at 4-5 cm below the hypocotyl, but the nodules on M. sativa formed almost 4 weeks later than those on M. murex. The difference in nodulation response between M. murex and M. sativa was related to numbers of S. medicae in the rhizosphere. After 24 d growth in soil of pHCaCl2 4.3, there were 100-fold higher numbers of S. medicae WSM419 associated with the roots of M. murex than M. sativa. This difference in rhizobial numbers was not due to differences in root growth as there were similar rates of root elongation in M. murex and M. sativa, or differences in the root products released as root exudates of M. murex and M. sativa produced at low pH had no significant effect on the growth of S. medicae. Using a ‘root mat’ approach on soil disks of pHCaCl2 4.49, M. sativa acidified its rhizosphere by approximately 0.2 0.4 pH-units within 4 d, while M. murex did not acidify its rhizosphere. Rates of H+ release were higher from M. sativa than from M. murex. Using videodensitometry with agarose of pH 4.5, ature parts of the tap-root of both pecies exuded OH– ions, but this was pproximately 2-times higher in M. murex than in M. sativa. Consequently, oung parts of the M. sativa rhizosphere were more acidic than hat of . murex. The higher rate of acidification by the oots of M. sativa made its hizosphere less favourable for the survival and growth of S. medicae. oot hair development was initially similar for both M. murex and . sativa. However by 7 d after sowing in soil of pHCaCl2 .3, he density of root airs on M. murex increased to 37 root hairs mm-1 root, while the density of root airs on M. sativa decreased to 20 root hairs mm-1 root. Due to higher oot hair density, the roots of M. murex provided a greater surface area for the attachment and colonisation of S. medicae compared to the roots of M. sativa. Indeed, confocal laser scanning microscopy at 7 d after sowing showed there were larger populations of a green fluorescent protein-marked transconjugant of S. medicae WSM419 colonised at 4-5 cm below the hypocotyl on the root of M. murex (3.28 pixel intensity units) compared to M. sativa (1.78 pixel intensity units). The smaller population of S. medicae colonised on the M. sativa root resulted in the observed delay in nodule development in M. sativa compared to M. murex. Two plant mechanisms contributed to the greater numbers of S. medicae in the M. murex rhizosphere compared to M. sativa rhizosphere when plants were grown in an acidic soil: (1) roots of M. murex had a higher density of root hairs, and thus provided a larger root surface area for the growth and colonisation of S. medicae than M. sativa, and (2) roots of M. murex acidified the rhizosphere less, and thus provided more favourable conditions for the growth and colonisation of S. medicae than the rhizosphere of M. sativa. Models explaining the different nodulation responses between M. murex and M. sativa in soil of pHCaCl2 4.3 and 7.0 are presented.
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Cheng, Yvonne. "Plant mechanisms contributing to acid impairment of nodulation of Medicago murex and Medicago sativa by Sinorhizobium medicae." Thesis, Cheng, Yvonne (2003) Plant mechanisms contributing to acid impairment of nodulation of Medicago murex and Medicago sativa by Sinorhizobium medicae. PhD thesis, Murdoch University, 2003. https://researchrepository.murdoch.edu.au/id/eprint/655/.

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The widespread sowing of the perennial forage legume Medicago sativa to lower groundwater tables in south-western Australia is limited as many soils targeted for its use are too acidic (pHCaCl2 < 5) for consistent nodulation with Sinorhizobium spp. The annual medic M. murex grows and nodulates well in these acidic soils, but it cannot fill the niche of M. sativa in lowering groundwater tables. The differential ability of M. murex and M. sativa to nodulate in acid soils provided the opportunity to compare the nodulation responses between the two species and to identify the mechanisms contributing to the poor nodulation of M. sativa in soil of low pH. An initial glasshouse experiment compared the nodulation of M. murex cv. Zodiac and M. sativa cv. Aquarius with S. medicae strains WSM419 and CC169. Subsequent glasshouse and laboratory experiments used only the more acid-tolerant S. medicae strain WSM419. In the glasshouse in soil of pHCaCl2 4.3, the uppermost nodule on both M. murex and M. sativa formed at 4-5 cm below the hypocotyl, but the nodules on M. sativa formed almost 4 weeks later than those on M. murex. The difference in nodulation response between M. murex and M. sativa was related to numbers of S. medicae in the rhizosphere. After 24 d growth in soil of pHCaCl2 4.3, there were 100-fold higher numbers of S. medicae WSM419 associated with the roots of M. murex than M. sativa. This difference in rhizobial numbers was not due to differences in root growth as there were similar rates of root elongation in M. murex and M. sativa, or differences in the root products released as root exudates of M. murex and M. sativa produced at low pH had no significant effect on the growth of S. medicae. Using a 'root mat' approach on soil disks of pHCaCl2 4.49, M. sativa acidified its rhizosphere by approximately 0.2 0.4 pH-units within 4 d, while M. murex did not acidify its rhizosphere. Rates of H+ release were higher from M. sativa than from M. murex. Using videodensitometry with agarose of pH 4.5, ature parts of the tap-root of both pecies exuded OH- ions, but this was pproximately 2-times higher in M. murex than in M. sativa. Consequently, oung parts of the M. sativa rhizosphere were more acidic than hat of . murex. The higher rate of acidification by the oots of M. sativa made its hizosphere less favourable for the survival and growth of S. medicae. oot hair development was initially similar for both M. murex and . sativa. However by 7 d after sowing in soil of pHCaCl2 .3, he density of root airs on M. murex increased to 37 root hairs mm-1 root, while the density of root airs on M. sativa decreased to 20 root hairs mm-1 root. Due to higher oot hair density, the roots of M. murex provided a greater surface area for the attachment and colonisation of S. medicae compared to the roots of M. sativa. Indeed, confocal laser scanning microscopy at 7 d after sowing showed there were larger populations of a green fluorescent protein-marked transconjugant of S. medicae WSM419 colonised at 4-5 cm below the hypocotyl on the root of M. murex (3.28 pixel intensity units) compared to M. sativa (1.78 pixel intensity units). The smaller population of S. medicae colonised on the M. sativa root resulted in the observed delay in nodule development in M. sativa compared to M. murex. Two plant mechanisms contributed to the greater numbers of S. medicae in the M. murex rhizosphere compared to M. sativa rhizosphere when plants were grown in an acidic soil: (1) roots of M. murex had a higher density of root hairs, and thus provided a larger root surface area for the growth and colonisation of S. medicae than M. sativa, and (2) roots of M. murex acidified the rhizosphere less, and thus provided more favourable conditions for the growth and colonisation of S. medicae than the rhizosphere of M. sativa. Models explaining the different nodulation responses between M. murex and M. sativa in soil of pHCaCl2 4.3 and 7.0 are presented.
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Cheng, Yvonne. "Plant mechanisms contributing to acid impairment of nodulation of Medicago murex and Medicago sativa by Sinorhizobium medicae." Cheng, Yvonne (2003) Plant mechanisms contributing to acid impairment of nodulation of Medicago murex and Medicago sativa by Sinorhizobium medicae. PhD thesis, Murdoch University, 2003. http://researchrepository.murdoch.edu.au/655/.

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The widespread sowing of the perennial forage legume Medicago sativa to lower groundwater tables in south-western Australia is limited as many soils targeted for its use are too acidic (pHCaCl2 < 5) for consistent nodulation with Sinorhizobium spp. The annual medic M. murex grows and nodulates well in these acidic soils, but it cannot fill the niche of M. sativa in lowering groundwater tables. The differential ability of M. murex and M. sativa to nodulate in acid soils provided the opportunity to compare the nodulation responses between the two species and to identify the mechanisms contributing to the poor nodulation of M. sativa in soil of low pH. An initial glasshouse experiment compared the nodulation of M. murex cv. Zodiac and M. sativa cv. Aquarius with S. medicae strains WSM419 and CC169. Subsequent glasshouse and laboratory experiments used only the more acid-tolerant S. medicae strain WSM419. In the glasshouse in soil of pHCaCl2 4.3, the uppermost nodule on both M. murex and M. sativa formed at 4-5 cm below the hypocotyl, but the nodules on M. sativa formed almost 4 weeks later than those on M. murex. The difference in nodulation response between M. murex and M. sativa was related to numbers of S. medicae in the rhizosphere. After 24 d growth in soil of pHCaCl2 4.3, there were 100-fold higher numbers of S. medicae WSM419 associated with the roots of M. murex than M. sativa. This difference in rhizobial numbers was not due to differences in root growth as there were similar rates of root elongation in M. murex and M. sativa, or differences in the root products released as root exudates of M. murex and M. sativa produced at low pH had no significant effect on the growth of S. medicae. Using a 'root mat' approach on soil disks of pHCaCl2 4.49, M. sativa acidified its rhizosphere by approximately 0.2 0.4 pH-units within 4 d, while M. murex did not acidify its rhizosphere. Rates of H+ release were higher from M. sativa than from M. murex. Using videodensitometry with agarose of pH 4.5, ature parts of the tap-root of both pecies exuded OH- ions, but this was pproximately 2-times higher in M. murex than in M. sativa. Consequently, oung parts of the M. sativa rhizosphere were more acidic than hat of . murex. The higher rate of acidification by the oots of M. sativa made its hizosphere less favourable for the survival and growth of S. medicae. oot hair development was initially similar for both M. murex and . sativa. However by 7 d after sowing in soil of pHCaCl2 .3, he density of root airs on M. murex increased to 37 root hairs mm-1 root, while the density of root airs on M. sativa decreased to 20 root hairs mm-1 root. Due to higher oot hair density, the roots of M. murex provided a greater surface area for the attachment and colonisation of S. medicae compared to the roots of M. sativa. Indeed, confocal laser scanning microscopy at 7 d after sowing showed there were larger populations of a green fluorescent protein-marked transconjugant of S. medicae WSM419 colonised at 4-5 cm below the hypocotyl on the root of M. murex (3.28 pixel intensity units) compared to M. sativa (1.78 pixel intensity units). The smaller population of S. medicae colonised on the M. sativa root resulted in the observed delay in nodule development in M. sativa compared to M. murex. Two plant mechanisms contributed to the greater numbers of S. medicae in the M. murex rhizosphere compared to M. sativa rhizosphere when plants were grown in an acidic soil: (1) roots of M. murex had a higher density of root hairs, and thus provided a larger root surface area for the growth and colonisation of S. medicae than M. sativa, and (2) roots of M. murex acidified the rhizosphere less, and thus provided more favourable conditions for the growth and colonisation of S. medicae than the rhizosphere of M. sativa. Models explaining the different nodulation responses between M. murex and M. sativa in soil of pHCaCl2 4.3 and 7.0 are presented.
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Bernal, Libia Maritza. "Bio-engineering alfalfa (Medicago sativa L.) to accumulate fructans." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ61873.pdf.

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Rodrigues, Sandra Maria Gonçalves. "Caracterização de Sinorhizobium sp. isolado de Medicago sativa L." Master's thesis, Universidade de Aveiro, 2009. http://hdl.handle.net/10773/832.

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Mestrado em Biologia Molecular e Celular
A utilização de luzernas (Medicago sativa L. e Medicago polymorpha L.) em pastagens na zona mediterrânea requer a inoculação de sementes com estirpes de Sinorhizobium (S. meliloti e S. medicae) eficazes e bem adaptadas às condições de clima semi-árido que prevalecem nesta região. Este trabalho teve como objectivo caracterizar a população de Sinorhizobium de um solo do Alentejo (Elvas-ENMP), isolada de nódulos de diferentes cultivares de M. sativa (ABT, Coussouls, Magali, Melissa e Mamuntanas), através da avaliação da diversidade genética e da eficácia simbiótica, bem como da tolerância a temperaturas elevadas (28 a 42ºC) e à salinidade (0 a 1000 mM de NaCl). A análise dos perfis obtidos mediante REP e ERIC PCR permitiram determinar a existência de diversos “clusters”, indicando uma elevada diversidade genética entre a população de Sinorhizobium estudada. Os resultados obtidos com os perfis de restrição do 16S rDNA, utilizando a enzima RsaI demonstraram uma prevalência de estirpes de S. medicae (73) em relação às estirpes de S. meliloti (18). Nos restantes parâmetros o comportamento das estirpes variou consoante a sua origem. Verificou-se a existência dum maior número de estirpes isoladas das cultivares Melissa e Mamuntanas com eficácia elevada, quando em simbiose com M. polymorpha e moderada com M. sativa, contrariamente às isoladas da cultivar Coussouls, que foram as menos eficazes com as duas espécies de Medicago. Relativamente à temperatura, foi na temperatura de 40ºC que as estirpes tiveram comportamentos mais diferenciados desde muito sensíveis a muito tolerantes, existindo um grande número de estirpes das cultivares Coussouls, Melissa e Mamuntanas entre as últimas. Apenas duas estirpes toleraram a temperatura de 42ºC, uma isolada de Coussouls e outra de Melissa. Nos ensaios de salinidade, a maioria das estirpes tolerou a concentração de 500 mM e sete toleraram a concentração de 1 M. Da totalidade de estirpes estudadas, apenas uma estirpe Melissa 5c apresentou elevada tolerância à temperatura e salinidade e simultaneamente uma eficácia simbiótica moderada. ABSTRACT: The use of alfalfa (Medicago sativa, L. and Medicago polymorpha) in pastures in the Mediterranean area implies the inoculation of seeds with Sinorhizobium (S. meliloti and S. medicae) bacteria effective and well adapted to the semi-arid environmental conditions which prevails in this area. The aim of this work was to characterize S. meliloti natural population present in an Alentejo soil (Elvas- ENMP), isolated from nodules of different M. sativa varieties (ABT, Coussouls, Magali Mamuntanas and Melissa). Genetic diversity and symbiotic efficiency as well as the tolerance to high temperatures (28 to 42ºC) and salinity (0 to 1000 mM of NaCl) were evaluated. Analyzing the different patterns obtained by REP and ERIC PCR, the existence of several clusters was verified, indicating a high genetic diversity among Sinorhizobium population studied. The results obtained with RsaI enzyme showed a prevalence of S. medicae strains (73) in relation to S. meliloti strains (18).Strains behaviour when facing high temperatures or salinity varied according to the host cultivar of origin. It was verified that a great number of strains isolated from cultivar Melissa and Mamuntanas had a high effectiveness, when in symbiosis with M. polymorpha and moderated with M. sativa, whereas strains isolated from cultivar Coussouls were poorly effective with both Medicago species. For tolerance to temperature, at 40ºC the strains had different behaviours from very sensitive to very tolerant, existing a great number of strains from the cultivar Coussouls, Melissa and Mamuntanas among the last ones. Only two strains tolerated the temperature of 42ºC, one isolated from Coussouls and another from Melissa. Salinity tests showed that almost all strains tolerate 500 mM, although seven tolerate 1 M. Among the strains studied, only one (Melissa 5c) has overcome the tests of tolerance, achieving medium values of nitrogen fixation.
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McKimmie, Timothy Irving 1948. "CHARACTERIZATION OF SALT TOLERANCE IN ALFALFA (MEDICAGO SATIVA L.)." Thesis, The University of Arizona, 1986. http://hdl.handle.net/10150/276348.

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Santos, Ana Raquel da Silva. "Biotoxicity assays of quantum dots in in vitro cultures of Medicago sativa and Medicago truncatula." Master's thesis, FCT - UNL, 2009. http://hdl.handle.net/10362/2295.

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Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para obtenção do grau de Mestre em Biotecnologia
The aim of this thesis is a survey of the toxicity of Quantum Dots (QD’s) in in vitro cultures of the legumes Medicago sativa and Medicago truncatula. Two samples of QD’s were used to assess toxicity: QD50_MPA tested in a concentration of [160 nM] and QD68_MPA with [40 nM]. The QD50_MPA sample was applied in the already available embryogenic cell suspension culture of M. truncatula (M9-10a) to evaluate the effect of QD’s in plant differentiation at somatic embryogenesis. Results showed no differences in embryo morphology or development time. A fine suspension culture of Medicago sativa (line M699) was established to evaluate the effect of QD68_MPA QD’s in cell growth, viability as well in oxidative stress by ROS production. It was concluded that no major differences were seen when cells were exposed to QD’s in a period of 4 hours, despite an overall oxidative stress was detected. After 24 hours of exposure of cell suspension cultures to QD’s viability of cells started to decrease and ROS production was intensified, also the morphology of cells had significant changes. Moreover, it was visualized the internalization of both QD´s samples after 72 h of cell suspension cultures exposure to QD’s. QD50_MPA sample was used in the two cell suspension cultures used: M. sativa and M. truncatula, and in both lines QD’s were visualized in the nucleus and in cytosol, with the exception of the vacuoles. QD68_MPA sample was found to be up-taked by M. sativa cells and located mainly in the hyaloplasm and organelles such as plasts, and similarly to the other sample, no internalization was observed in the vacuoles.
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Gregory, Abigail C. E. "The metabolism of isoflavonoid phytoalexins in alfalfa (Medicago sativa L.)." Thesis, Durham University, 1995. http://etheses.dur.ac.uk/5422/.

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The synthesis of isoflavonoid phytoalexins in legumes is relatively well understood, but far less is known about how these phytotoxic compounds are metabolised by the plant when no longer required. In this project medicarpin, the major isoflavonoid phytoalexin in alfalfa, was prepared in radiolabelled form and fed to cell cultures and seedlings of alfalfa. The metabolism of the radioactive phytoalexin was then studied by characterising the radiolabelled metabolites formed. Uptake of radiolabelled phytoalexin by cells was faster in elicitor-treated cultures than in untreated cultures. However, there was little difference in pattern or speed of metabolism in treated or untreated cultures. Labelled medicarpin was rapidly metabolised to a complex range of extractable medicarpin products (MPs). A very small proportion of the dose was broken down to (^14)C02. A total of 8 MPs could be resolved as distinct metabolites by HPLC and TLC. However, as incubation time increased the radioactivity became associated with multiple minor components which could not be identified. The 8 MPs were characterised by UV and mass-spectrometry and where possible by co- chromatography with authentic standards by TLC and HPLC. Four MPs were unambiguously identified as medicarpin-3-0-glucoside-6"-O-malonate (MGM), the isoflavans vestitol and sativan and the pterocarpan 6a-hydroxy-3,4'- dimethoxypterocarpan (variabilin). In addition a hydroxylated derivative of medicarpin, termed pseudomedicarpin was also tentatively identified. Of the four remaining metabolites MPl had a relative molecular mass of 166 but remained unidentified. MP2 was formed from pseudomedicarpin, but could not be characterised due to its labile nature. Similarly MP3 and MP6 remained unidentified, though the evidence suggested that MPS was a demethylated product of medicarpin. The metabolism of medicarpin in seedlings resembled that of cell cultures with the exception that rather more of the medicarpin was conjugated to form MGM.
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Ramachandran, Rupesh Ram Kariyat. "Alternate methods for cultivar synthesis in alfalfa (Medicago sativa L.)." Laramie, Wyo. : University of Wyoming, 2007. http://proquest.umi.com/pqdweb?did=1400950151&sid=1&Fmt=2&clientId=18949&RQT=309&VName=PQD.

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Books on the topic "Medicago sativa"

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Manchanda, R. K. Monograph on homoeopathic drug alfalfa (medicago sativa). Edited by Central Council for Research in Homoeopathy (India). New Delhi, India: Central Council for Research in Homoeopathy, 2014.

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1852-1908, Fletcher James, Shutt Frank T. 1859-1940, and Dominion Experimental Farms and Stations (Canada), eds. Alfalfa or lucern (Medicago sativa, L.): Its culture, use and value. Ottawa: Dept. of Agriculture, 1997.

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Wasser, Clinton H. Alfalfa (Medicago sativa): Section 7.3.1, US Army Corps of Engineers wildlife resources management manual. Vicksburg, Miss: U.S. Army Engineer Waterways Experiment Station, 1986.

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Klimenko, Irina, Nikolay Kozlov, Sergey Kostenko, Anastasia Shamustakimova, and Yulian Mavlyutov. Identification and certification of forage grasses (meadow clover, alfalfa, sowing and hop) based on DNA markers. ru: Federal Williams Research Center of Forage Production and Agroecology, 2020. http://dx.doi.org/10.33814/978-5-6043194-9-9.

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A technology has been developed for DNA identification and certification of varieties of meadow clover (Trifolium pratense L.), alfalfa (Medicago varia Mart.), Sowing (M. sativa L.) and hop (M. lupuli-na L.) based on molecular analysis with using SSR and SRAP markers. The recommendations contain a description of the sequence of experiments and protocols for DNA typing procedures. The presented methods were developed by the authors on the basis of their own experimental research and using the data available in the literature. A characteristic of informative primers for each marking system is given, a set of DNA identification markers is proposed, and unique molecular genetic formulas of varieties are drawn up as the basis for a reference genetic passport. Methodological recommendations were prepared with the aim of mastering the technology of DNA certification of forage grasses in practice. Designed for managers and specialists of research and control laboratories, can serve as a textbook for students and postgraduates in specialized specialties.
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Migliorini, Paola. La terminologia medica come strumento espressivo della satira di Persio. [Pistoia]: [Quaderni di Anazetesis], 1990.

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Baḍonī, Arūṇa Kumāra. Gaṛhavāla Himālaya meṃ jaivika vividhatā saṃrakshaṇa evaṃ satata vikāsa hetu jaṛī-būṭī udyoga. Deharādūna: Sosāyaṭī phôra Himālayana Envāiranamenṭala Risarca, 1995.

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Newman, Art. The illustrated treasury of medical curiosa. New York: McGraw-Hill Book Co., 1988.

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Richard, Gordon. Great medical mysteries. London: Arrow, 1985.

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Gordon, Richard. Great medical disasters. New York: Barnes & Noble, 1995.

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Kivistö, Sari. Medical analogy in Latin satire. New York: Palgrave Macmillan, 2009.

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Book chapters on the topic "Medicago sativa"

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Bährle-Rapp, Marina. "Medicago sativa." In Springer Lexikon Kosmetik und Körperpflege, 344. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_6380.

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De Smet, P. A. G. M. "Medicago sativa." In Adverse Effects of Herbal Drugs, 161–70. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-49340-9_13.

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Khan, Nabeeha Aslam, Imran Ul Haq, Siddra Ijaz, and Barbaros Cetinel. "Medicago sativa." In Sustainable Winter Fodder, 83–110. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003055365-6.

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Khare, C. P. "Medicago sativa Linn." In Indian Medicinal Plants, 1. New York, NY: Springer New York, 2007. http://dx.doi.org/10.1007/978-0-387-70638-2_986.

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Azimova, Shakhnoza S., and Anna I. Glushenkova. "Medicago sativa L." In Lipids, Lipophilic Components and Essential Oils from Plant Sources, 586–87. London: Springer London, 2012. http://dx.doi.org/10.1007/978-0-85729-323-7_1915.

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Paniagua-Zambrana, Narel Y., Rainer W. Bussmann, and Carolina Romero. "Medicago sativa L. Fabaceae." In Ethnobotany of Mountain Regions, 1–5. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-319-77093-2_186-1.

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Paniagua-Zambrana, Narel Y., Rainer W. Bussmann, and Carolina Romero. "Medicago sativa L. Fabaceae." In Ethnobotany of Mountain Regions, 1191–95. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-28933-1_186.

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Sastry, K. Subramanya, Bikash Mandal, John Hammond, S. W. Scott, and R. W. Briddon. "Medicago sativa (Alfalfa/Lucerne)." In Encyclopedia of Plant Viruses and Viroids, 1508–22. New Delhi: Springer India, 2019. http://dx.doi.org/10.1007/978-81-322-3912-3_579.

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Jan, Hammand Ahmad, Wahid Hussain, Ripu M. Kunwar, Rainer W. Bussmann, and Narel Y. Paniagua-Zambrana. "Medicago sativa L. Fabaceae." In Ethnobotany of the Himalayas, 1–7. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-45597-2_150-1.

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Fu, Chunxiang, Timothy Hernandez, Chuanen Zhou, and Zeng-Yu Wang. "Alfalfa (Medicago sativa L.)." In Methods in Molecular Biology, 213–21. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1695-5_17.

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Conference papers on the topic "Medicago sativa"

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Kurenkova E.М., E. М., and N. N. Lazarev N. N. "Sustainability of different varieties of alfalfa on soddy-podzoly soils with long-term use." In Растениеводство и луговодство. Тимирязевская сельскохозяйственная академия, 2020. http://dx.doi.org/10.26897/978-5-9675-1762-4-2020-23.

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Stepanova, Galina. "ALFALFA VARIETIES ZONED IN THE CENTRAL CHERNOZEM ZONE OF THE RUSSIAN FEDERATION." In Multifunctional adaptive fodder production. ru: Federal Williams Research Center of Forage Production and Agroecology, 2020. http://dx.doi.org/10.33814/mak-2020-24-72-64-78.

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The article describes the main morphological and biological features of alfalfa varieties included in the State register of breeding achievements approved for use in the Central Chernozem zone of Russia. A total of 32 alfalfa varieties are included in the State register. This is 9 varieties of blue alfalfa (Medicago sativa L. subsp. Sativa) of domestic selection and 8 foreign, 11 varieties of variable alfalfa (Medicago sativa L. nothosubsp. varia (Martyn) Arcang.) domestic selection and 1 variety of foreign and 3 varieties of yellow alfalfa (Medicago sativa L. subsp. falcata (L.) Arcang.). It shows the average and maximum yield of varieties determined in the process of state variety testing, as well as independent evaluation in research institutions in the region. Varieties of blue alfalfa of domestic selection Kevsala, Elena, Satellite, Vavilovskaya Yubileynaya were the most productive. The average yield of dry matter of these varieties reaches 8.4–9.2 t/ha, the maximum — 15.3–17.7 t/ha. Alfalfa varieties Timbale and Galaxy were the most productive among foreign varieties: the average yield of dry matter was 8.1 and 8.3 t/ha, the maximum – 15.5 and 17.2 t/ha. Varieties of alfalfa variable Vitalina and Vela provided an average yield of dry matter of 7.6 and 9.0 t/ha, the maximum yield reached 15.4 and 18.1 t/ha.
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"Some nutritional and physical properties of alfalfa (Medicago sativa Linn) seeds." In 2016 ASABE International Meeting. American Society of Agricultural and Biological Engineers, 2016. http://dx.doi.org/10.13031/aim.20162461889.

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Fokapić, S., I. Bikit, D. Mra, M. Vesković, J. Slivka, Ž Mihaljev, and Ž Ćupić. "Low Level Gamma Spectroscopy Measurements of Radium and Cesium in Lucerne (Medicago Sativa)." In SIXTH INTERNATIONAL CONFERENCE OF THE BALKAN PHYSICAL UNION. AIP, 2007. http://dx.doi.org/10.1063/1.2733205.

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Yin, Peng, Yue Wu, Liang Zi, and Xia Hu. "Effects of different base fertilizers on enzyme activities in Medicago sativa rhizosphere soil." In 4TH INTERNATIONAL CONFERENCE ON FRONTIERS OF BIOLOGICAL SCIENCES AND ENGINEERING (FBSE 2021). AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0094788.

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Sorina, Popescu. "THE KNOX GENES INVOLVEMENT IN THE DEVELOPMENT OF MULTILEAFLED TRAIT ON TETRAPLOID MEDICAGO SATIVA." In 14th SGEM GeoConference on NANO, BIO AND GREEN � TECHNOLOGIES FOR A SUSTAINABLE FUTURE. Stef92 Technology, 2014. http://dx.doi.org/10.5593/sgem2014/b61/s25.075.

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Tirry, Nabil, Mohamed Ferioun, Aziza Kouchou, Ghizlane Laghmari, Wifak Bahafid, and Naïma El Ghachtouli. "Enhanced Salinity Tolerance of Medicago sativa, Roots AM Colonization and Soil Enzyme Activities by PGPR." In LAFOBA2. Basel Switzerland: MDPI, 2022. http://dx.doi.org/10.3390/environsciproc2022016014.

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Ptashec, Ol'ga, and Lyudmila Luchenok. "Influence of the method of sowing and seeding rate on the yield of alfalfa in the south of Belarus." In Multifunctional adaptive fodder production23 (71). ru: Federal Williams Research Center of Forage Production and Agroecology, 2020. http://dx.doi.org/10.33814/mak-2020-23-71-52-54.

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The results of studies on the influence of agrotechnological techniques on the productivity of alfalfa (Medicago sativa), cultivation on agro-peat soils in the south of Belarus were presented. It was established that the yield of green mass on average over 4 years of life was 393.6–517.9 c / ha when sown under cover and 494.3–629.8 centners ha-1 with coverless sowing. Productivity — 56.5–75.1 and 69.5–89.7 centners ha-1, respectively. A positive correlation between the increase in the yield and productivity of the grass stand with an rise in the seeding rate and doses of fertilizers applied was noted.
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Sherifi, Enver, Naser Shabani, and Nezhda Sherifi. "Heavy Metal Contamination of Roadside Soils and Their Influence on Vascular Boundles to Medicago Sativa L." In University for Business and Technology International Conference. Pristina, Kosovo: University for Business and Technology, 2017. http://dx.doi.org/10.33107/ubt-ic.2017.180.

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Zhao, Chunhua, Jianming Wu, Ziyong Cheng, and Shangli Shi. "Research on mechanical properties and chemical compositions of bottom stems of medicago sativa in harvesting period." In 2011 International Conference on Information Science and Technology (ICIST). IEEE, 2011. http://dx.doi.org/10.1109/icist.2011.5765088.

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Reports on the topic "Medicago sativa"

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Bridson, N. P. The photosynthetic and stomatal response of Medicago sativa cv. saranac to free-air CO{sub 2} enrichment (F.A.C.E.) and nitrogen. Office of Scientific and Technical Information (OSTI), August 1996. http://dx.doi.org/10.2172/380353.

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Kapulnik, Yoram, and Donald A. Phillips. Isoflavonoid Regulation of Root Bacteria. United States Department of Agriculture, January 1996. http://dx.doi.org/10.32747/1996.7570561.bard.

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The overall objective of this project was to develop a conceptual framework for enhancing root colonization by beneficial bacteria. To accomplish this aim we tested the hypothesis that production and excretion of the plant phytoalexin medicarpin can be used for creation of a special niche along the legume roots, where beneficial microorganism, such as rhizobium, will have a selective advantage. On the Israeli side it was shown that higher medicarpin levels are exuded following the application of Rhizobium meliloti to the rhizosphere but the specific biochemical pathway governing medicarpin production was not induced significantly enough to support a constant production and excretion of this molecule to the rhizosphere. Furthermore, pathogenic bacteria and chemical elicitors were found to induce higher levels of this phytoalexin and it became important to test its natural abundance in field grown plants. On the US side, the occurrence of flavonoids and nucleosides in agricultural soils has been evaluated and biologically significant quantities of these molecules were identified. A more virulent Agrobacterium tumefaciens strain was isolated from alfalfa (Medicago sativa L.) which forms tumors on a wide range of plant species. This isolate contains genes that increase competitive colonization abilities on roots by reducing the accumulation of alfalfa isoflavonoids in the bacterial cells. Following gene tagging efforts the US lab found that mutation in the bacterial efflux pump operons of this isolate reduced its competitive abilities. This results support our original hypothesis that detoxification activity of isoflavenoids molecules, based on bacterial gene(s), is an important selection mechanism in the rhizosphere. In addition, we focused on biotin as a regulatory element in the rhizosphere to support growth of some rhizosphere microorganisms and designed a bacterial gene construct carrying the biotin-binding protein, streptavidin. Expressing this gene in tobacco roots did not affect the biotin level but its expression in alfalfa was lethal. In conclusion, the collaborative combination of basic and applied approaches toward the understanding of rhizosphere activity yielded new knowledge related to the colonization of roots by beneficial microorganisms in the presence of biological active molecules exuded from the plant roots.
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