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Relevant bibliographies by topics / Mammalian cells

Academic literature on the topic 'Mammalian cells'

Author: Grafiati

Published: 4 June 2021

Last updated: 8 June 2024

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Journal articles on the topic "Mammalian cells"

1

Terskikh, Alexey V., Peter J. Bryant, and Philip H. Schwartz. "Mammalian Stem Cells." Pediatric Research 59 (April 2006): 13R—20R. http://dx.doi.org/10.1203/01.pdr.0000205154.86517.2a.

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Francke, Uta. "Mammalian Cells Mammalian Cell Genetics Martin L. Hooper." BioScience 37, no. 10 (November 1987): 741–42. http://dx.doi.org/10.2307/1310484.

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Kordium, V. A., S. P. Shpilevaya, T. A. Ruban, O. M. Sukhorada, and V. I. Andriyenko. "Autotransformation of mammalian cells." Biopolymers and Cell 21, no. 2 (March 20, 2005): 140–44. http://dx.doi.org/10.7124/bc.0006e4.

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Haas, Michael J. "mAbs from mammalian cells." Science-Business eXchange 1, no. 36 (October 2008): 869. http://dx.doi.org/10.1038/scibx.2008.869.

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Vinson, Valda. "Sounding out mammalian cells." Science 365, no. 6460 (September 26, 2019): 1414.5–1415. http://dx.doi.org/10.1126/science.365.6460.1414-e.

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Abounit, Kadija, Tiziano M. Scarabelli, and Roy B. McCauley. "Autophagy in mammalian cells." World Journal of Biological Chemistry 3, no. 1 (2012): 1. http://dx.doi.org/10.4331/wjbc.v3.i1.1.

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Rodriguez-Osorio, N., R. Urrego, J. B. Cibelli, K. Eilertsen, and E. Memili. "Reprogramming mammalian somatic cells." Theriogenology 78, no. 9 (December 2012): 1869–86. http://dx.doi.org/10.1016/j.theriogenology.2012.05.030.

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Peel, A. "Transfection of mammalian cells." Methods 33, no. 2 (June 2004): 93–94. http://dx.doi.org/10.1016/j.ymeth.2003.11.001.

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Pines, Jonathon. "GFP in mammalian cells." Trends in Genetics 11, no. 8 (August 1995): 326–27. http://dx.doi.org/10.1016/s0168-9525(00)89092-7.

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Cogoli, A. "Microgravity and mammalian cells." Cell Differentiation and Development 27 (August 1989): 180. http://dx.doi.org/10.1016/0922-3371(89)90545-5.

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Dissertations / Theses on the topic "Mammalian cells"

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Iqbal, Syed Amir. "Asymmetric Cell Division in Mammalian Cells." Thesis, University of Manchester, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.503635.

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Lau, Stephen S. K. "Gene silencing in mammalian cells." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ28435.pdf.

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Renglin, Lindh Anna. "Mitotic aberrations in mammalian cells /." Stockholm : Dept. of genetics, microbiology and toxicology, Stockholm university, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-522.

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Xue, Yue 1978. "Iron metabolism in mammalian cells." Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=79216.

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Iron, known for its versatility, is an essential element in the metabolism of mammalian cells. One of the most common iron disorders is autosomal recessive disease---hereditary hemochromatosis, which leads to the iron overload in population of northern European descent. During years of my graduate research, I focused on the study of Hemochromatosis gene Hfe and a point mutation C282Y that leads to more than 80% of all hemochromatosis cases.
Iron Regulatory Proteins (IRPs), which serve as main posttranscriptional regulators of cellular iron homeostasis, are the other interest of research. Iron regulatory proteins reversibly interact with iron regulatory elements (IREs) within ferritin and transferrin receptor (TfR) mRNAs. The binding ability of IRPs is under tight control so that they respond to the changes in the intracellular iron requirements in a coordinate manner by differentially regulating ferritin mRNA translational efficiency and TfR mRNA stability. Besides intracellular iron levels, some other stimuli, such as oxidative stress, are capable of regulating this RNA-protein interactions.

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Hawley, Patricia. "Oligodeoxynucleotide interaction with mammalian cells." Thesis, University of East Anglia, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338058.

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Gohari, Nasrollah Saleh. "Homologous recombination in mammalian cells." Thesis, University of Sheffield, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.414660.

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Jiang, Lei. "Mitochondrial Distribution in Mammalian Cells." University of Dayton / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1259968456.

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Heikinheimo, Liisa. "Phosphatidylserine translocation in mammalian cells." Helsinki : University of Helsinki, 2002. http://ethesis.helsinki.fi/julkaisut/mat/bioti/vk/heikinheimo/.

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Bekei, Beata [Verfasser]. "In-cell NMR Spectroscopy in Mammalian Cells / Beata Bekei." Berlin : Freie Universität Berlin, 2013. http://d-nb.info/1043198059/34.

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Fengler, John Josef Paul. "Respiration induced oxygen gradients in cultured mammalian cells." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/28381.

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Oxygen is known to sensitize X-irradiated cells to lethal radiation damage. At low ambient oxygen tensions, however, the molecular mechanisms of the sensitization process and the metabolic requirements of the cell may be forced to compete for the cellular oxygen supply. The effect of cell respiration on the availability of intracellular oxygen during irradiation was consequently investigated by comparing the radiosensitivities of respiring and non-respiring cells. Cultured mammalian cells were irradiated in single cell suspensions and thin film monolayers at respiration inhibiting (4°C) and at normal cell culturing (37°C) temperatures. Due to oxygen equilibration and radiolytic depletion problems, the results of the suspension culture experiments were inconclusive. By subsequently analyzing the diffusive mass transfer of oxygen in the suspension medium, the stirrer flask was determined to be an inappropriate culture vessel in which to irradiate cells at constant low oxygen concentrations. A thin film cell culture system in which the oxygen concentrations to which the cells were exposed during irradiation could be more accurately controlled was then developed. A comparison of the oxygen enhanced radiosensitivities of the respiring and non-respiring cells in thin film monolayers suggested that the metabolic depletion of oxygen at low oxygen tensions has a significant effect on the local and intracellular oxygen distribution. These effects are representative of those that would be produced if respiration induced oxygen gradients existed inside and immediately around respiring cells. The magnitude of the differential radiosensitivities was found to be dependent on cell shape and to have values that agreed very well with theoretical predictions based on the existence of such gradients.
Science, Faculty of
Physics and Astronomy, Department of
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Books on the topic "Mammalian cells"

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Edgerton, Jane. Electroporation of mammalian cells. Birmingham: University of Birmingham, 1992.

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C, Heiser William, ed. Gene delivery to mammalian cells. Totowa, N.J: Humana Press, 2004.

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Heiser, William C. Gene Delivery to Mammalian Cells. New Jersey: Humana Press, 2003. http://dx.doi.org/10.1385/1592596495.

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Heiser, William C. Gene Delivery to Mammalian Cells. New Jersey: Humana Press, 2003. http://dx.doi.org/10.1385/1592596509.

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Hartley, James L., ed. Protein Expression in Mammalian Cells. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-352-3.

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Simpson, Nicholas. Metabolic engineering in mammalian cells. Birmingham: University of Birmingham, 1999.

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S, Gupta Radhey, ed. Drug resistance in mammalian cells. Boca Raton, Fla: CRC Press, 1989.

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M, Gottesman Michael, ed. Molecular genetics of mammalian cells. San Diego: Academic Press, 1987.

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1940-, Dexter Daniel L., ed. Mammalian tumor cell heterogeneity. Boca Raton, Fla: CRC Press, 1986.

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Hacker, David L., ed. Recombinant Protein Expression in Mammalian Cells. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8730-6.

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Book chapters on the topic "Mammalian cells"

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Sandig, Volker, Thomas Rose, Karsten Winkler, and Rene Brecht. "Mammalian Cells." In Production of Recombinant Proteins, 233–52. Weinheim, FRG: Wiley-VCH Verlag GmbH & Co. KGaA, 2005. http://dx.doi.org/10.1002/3527603670.ch11.

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Tzfira, Tzvi, Talya Kunik, Yedidya Gafni, and Vitaly Citovsky. "Mammalian Cells." In Agrobacterium Protocols Volume 2, 435–51. Totowa, NJ: Humana Press, 2006. http://dx.doi.org/10.1385/1-59745-131-2:435.

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Ostheimer, Gerard J. "Cell Cycle of Mammalian Cells." In Encyclopedia of Systems Biology, 303–5. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-9863-7_20.

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Escobar, María Luisa, Gerardo H. Vázquez-Nin, and Olga M. Echeverría. "Prefollicular Cells." In Cell Death in Mammalian Ovary, 165–70. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-1134-1_10.

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Escobar, María Luisa, Gerardo H. Vázquez-Nin, and Olga M. Echeverría. "Follicular Cells." In Cell Death in Mammalian Ovary, 185–200. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-1134-1_12.

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O’Connor, Patrick M., and Joany Jackman. "Synchronization of Mammalian Cells." In Cell Cycle — Materials and Methods, 63–74. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-57783-3_6.

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Pörtner, R. "Bioreactors for Mammalian Cells." In Cell Engineering, 89–135. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-10320-4_4.

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Pfizenmaier, Jennifer, and Ralf Takors. "Host Organisms: Mammalian Cells." In Industrial Biotechnology, 643–71. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2016. http://dx.doi.org/10.1002/9783527807796.ch17.

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Swartz, Randall W. "Mammalian Cells as Factories." In The Impact of Chemistry on Biotechnology, 102–20. Washington, DC: American Chemical Society, 1988. http://dx.doi.org/10.1021/bk-1988-0362.ch008.

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Bligh, J. "Cells, Cell-Talk and Mammalian Homeothermy." In Thermoreception and Temperature Regulation, 163–73. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-75076-2_16.

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Conference papers on the topic "Mammalian cells"

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Aksan, Alptekin, and Mehmet Toner. "Measurement of Molecular Mobility in Mammalian Cells." In ASME 2004 International Mechanical Engineering Congress and Exposition. ASMEDC, 2004. http://dx.doi.org/10.1115/imece2004-61508.

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Preservation of mammalian cells requires establishing a reversible stasis condition by reducing the intra/extracellular molecular mobility ensuring reduced chemical reaction and deterioration rates. Molecular mobility may be reduced by various techniques. For example, in cryopreservation, mobility within and surrounding the cell is reduced through freezing the free water that constitutes 70–90% of the cell’s composition. In dried-state preservation applied successfully to preserve seeds, pharmacological materials and foodstuff (mimicking the anhydrobiosis phenomenon seen in nature), reduction in molecular mobility is established by removing intra/extracellular water. Certain carbohydrates (such as trehalose and sucrose) can be artificially uploaded into mammalian cells to replace the removed water and to form an intra/extracellular glass. In this research, a fluorescent rotor is utilized to determine the changes in intracellular molecular mobility during carbohydrate uploading of mammalian cells. It was shown that using this technique, it is feasible to make real-time mobility measurements at a single cell level.

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Stevenson, David, Ben Agate, Lynn Paterson, Tanya Lake, Muriel Comrie, Tom Brown, Andrew Riches, et al. "Optical transfection of mammalian cells." In Photonics Europe, edited by Romualda Grzymala and Olivier Haeberle. SPIE, 2006. http://dx.doi.org/10.1117/12.662325.

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Munoz, S., J. L. Sebastian, M. Sancho, J. M. Miranda, and B. Ribas. "SAR Distribution In Cylindrical Mammalian Cells." In 30th European Microwave Conference, 2000. IEEE, 2000. http://dx.doi.org/10.1109/euma.2000.338624.

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Wha Lin, Shu, J. Ware, H. Roberts, N. McGraw, W. McAllister, and D. Stafford. "EXPRESSION OF HUMAN FACTOR IX IN MAMMALIAN CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643567.

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Human factor IX has been expressed in mammalian cells. A cloned factor IX cDNA missing the first 15 nucleotides of the 5’ end was modified by in vitro mutagenesis to restore the missing codons and add the translation consensus sequence, CCACC, proposed by Kozak to be optimal for translational initiation. Additionally, Bgl II and BamHI sites were added immediately upstream of the CCACC sequence for ease of portability of the fragment. This modified cDNA was inserted into a bovine papillomavirus (BPV) vector under the control of a mouse met alio thionein promoter. The constructed plasmid pBPV-IX was used to transfect a mouse fibroblast cell line C127. After 3 weeks, the transformed foci were isolated and the established cell lines were grown in the presence or absence of vitamin K. Media was collected at 3 day intervals and assayed for factor IX activity in a one stage clotting assay. A standard curve was constructed using purified human factor IX. Cells grown in the presence of vitamin K (3 mg/L) exhibited an activity equivalent to 350 ng/ml of factor IX in the cell media; no (less than 3 ng/ml) activity was detectable in the absence of vitamin K. A monoclonal antibody column specific for the Ca++ dependent form of human factor IX allowed the isolation of approximately 7 ug of purified factor IX from approximately 100 ml of culture medium. Western blot analysis of the purified factor IX revealed 2 protein bands which reacted with a goat anti-human factor IX antibody as well as a human specific monoclonal antibody. One of the immunoreactive bands migrates with authentic human factor IX and the other migrates slower. This expression system provides a convenient way to produce suitable amounts of factor IX and mutated factor IX protein for functional analyses.

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Hebert, Colin G., Carlos Lopez-Mariscal, and Alex Terray. "Antibody-Free Optical Analysis of Mammalian Cells." In Optical Trapping Applications. Washington, D.C.: OSA, 2013. http://dx.doi.org/10.1364/ota.2013.tw5d.2.

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Sabens, David. "Calcium Imaging of Sonoporation of Mammalian Cells." In THERAPEUTIC ULTRASOUND: 5th International Symposium on Therapeutic Ultrasound. AIP, 2006. http://dx.doi.org/10.1063/1.2205531.

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Watt, Fiona M. "Abstract IA06: Stem cells in mammalian epidermis." In Abstracts: AACR Special Conference on Developmental Biology and Cancer; November 30 - December 3, 2015; Boston, Massachusetts. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1557-3125.devbiolca15-ia06.

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Arita, Yoshihiko, Robert F. Marchington, David J. Stevenson, Frank J. Gunn-Moore, and Kishan Dholakia. "High Throughput Photoporation of Mammalian Cells using Microfluidic Cell Delivery." In Biomedical Optics. Washington, D.C.: OSA, 2010. http://dx.doi.org/10.1364/biomed.2010.btud92.

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Marchington, Robert F., Yoshihiko Arita, David J. Stevenson, Frank J. Gunn-Moore, and Kishan Dholakia. "High Throughput Photoporation of Mammalian Cells using Microfluidic Cell Delivery." In Conference on Lasers and Electro-Optics. Washington, D.C.: OSA, 2010. http://dx.doi.org/10.1364/cleo.2010.jmc6.

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He, Xiaoming, Eric Y. H. Park, Alex Fowler, Martin L. Yarmush, and Mehmet Toner. "Ultra-Fast Vitrification of Murine Embryonic Stem Cells Using a Low Concentration of Cryoprotectants." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192248.

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Effective cryopreservation of important mammalian cells and their derivatives is critical to the success of cell based medicine in 21st century [1]. There are currently two approaches to achieve cryopreservation of mammalian cells: conventional slow freezing and vitrification without ice formation. Although conventional slow freezing only requires a low relatively nontoxic concentration of cryoprotectants (1–2 M), it is always associated with cell injury due to ice formation and freeze concentration (i.e., solute effect) [2]. Cryopreservation by vitrification avoids ice formation all together. Existing protocols for vitrification, however, require a very high concentration of cryoprotectants (CPAs, generally more than 4M) that is usually toxic to most mammalian cells [3]. Therefore, it is of great interest to achieve vitrification using a low nontoxic concentration of cryoprotectants, which combines the advantages of the existing slow freezing and vitrification approaches while avoiding their shortcomings. In this study, we report the successful vitrification of murine embryonic stem (ES) cells at a low nontoxic level of cryoprotectants utilizing a thin walled (10μm) quartz microcapillary (outer diameter, 200μm). The ES cells post-vitrification retained high immediate viability, attachment efficiency and similar proliferation characteristic to fresh ES cells. Expression of markers characteristic to the ES cells suggests the ES cells retained the undifferentiated properties of pluripotent cells post-vitrification.

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Reports on the topic "Mammalian cells"

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Hannon, Gregory J. Complementation Screening in Mammalian Cells: Application to Cell Immortalization. Fort Belvoir, VA: Defense Technical Information Center, October 2000. http://dx.doi.org/10.21236/ada392208.

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Hannon, Gregory. Complementation Screening in Mammalian Cells: Application to Cell Immortalization. Fort Belvoir, VA: Defense Technical Information Center, October 1999. http://dx.doi.org/10.21236/ada384034.

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Brown, J. T., and W. Meyer-Ilse. Imaging mammalian cells with soft x rays: The importance of specimen preparation. Office of Scientific and Technical Information (OSTI), April 1997. http://dx.doi.org/10.2172/603453.

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Diamond, A. M., J. L. Murray, P. Dale, R. Tritz, and D. J. Grdina. The effects of selenium on glutathione peroxidase activity and radioprotection in mammalian cells. Office of Scientific and Technical Information (OSTI), September 1995. http://dx.doi.org/10.2172/510356.

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Albrecht-Buehler, Guenter, and Robert L. Rea. The Role of Mitochondria in the Detection of Infrared Light Sources by Mammalian Cells. Fort Belvoir, VA: Defense Technical Information Center, July 2005. http://dx.doi.org/10.21236/ada439696.

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Albrecht-Buehler, Guenter, and Robert L. Rea. The Role of Mitochondria in the Detection of Infrared Light Sources by Mammalian Cells. Fort Belvoir, VA: Defense Technical Information Center, January 2002. http://dx.doi.org/10.21236/ada403584.

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Albrecht-Buehler, Guenter, and Robert L. Rea. The Role of Mitochondria in the detection of Infrared Light Sources by Mammalian Cells. Fort Belvoir, VA: Defense Technical Information Center, March 2000. http://dx.doi.org/10.21236/ada376002.

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Sadot, Einat, Christopher Staiger, and Zvi Kam Weizmann. functional genomic screen for new plant cytoskeletal proteins and the determination of their role in actin mediated functions and guard cells regulation. United States Department of Agriculture, January 2003. http://dx.doi.org/10.32747/2003.7587725.bard.

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The original objectives of the approved proposal were: 1. To construct a YFP fused Arabidopsis cDNA library in a mammalian expression vector. 2. To infect the library into a host fibroblast cell line and to screen for new cytoskeletal associated proteins using an automated microscope. 3. Isolate the new genes. 4. Characterize their role in plants. The project was approved as a feasibility study to allow proof of concept that would entail building the YFP library and picking up a couple of positive clones using the fluorescent screen. We report here on the construction of the YFP library, the development of the automatic microscope, the establishment of the screen and the isolation of positive clones that are plant cDNAs encoding cytoskeleton associated proteins. The rational underling a screen of plant library in fibroblasts is based on the high conservation of the cytoskeleton building blocks, actin and tubulin, between the two kingdoms (80-90% homology at the level of amino acids sequence). In addition, several publications demonstrated the recognition of mammalian cytoskeleton by plant cytoskeletal binding proteins and vice versa. The major achievements described here are: 1. The development of an automated microscope equipped with fast laser auto-focusing for high magnification and a software controlling 6 dimensions; X, Y position, auto focus, time, color, and the distribution and density of the fields acquired. This system is essential for the high throughput screen. 2. The construction of an extremely competent YFP library efficiently cloned (tens of thousands of clones collected, no empty vectors detected) with all inserts oriented 5't03'. These parameters render it well representative of the whole transcriptome and efficient in "in-frame" fusion to YFP. 3. The strategy developed for the screen allowing the isolation of individual positive cDNA clones following three rounds of microscopic scans. The major conclusion accomplished from the work described here is that the concept of using mammalian host cells for fishing new plant cytoskeletal proteins is feasible and that screening system developed is complete for addressing one of the major bottlenecks of the plant cytoskeleton field: the need for high throughput identification of functionally active cytoskeletal proteins. The new identified plant cytoskeletal proteins isolated in the pilot screen and additional new proteins which will be isolated in a comprehensive screen will shed light on cytoskeletal mediated processes playing a major role in cellular activities such as cell division, morphogenesis, and functioning such as chloroplast positioning, pollen tube and root hair elongation and the movement of guard cells. Therefore, in the long run the screen described here has clear agricultural implications.

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Ehrlich, Marcelo, John S. Parker, and Terence S. Dermody. Development of a Plasmid-Based Reverse Genetics System for the Bluetongue and Epizootic Hemorrhagic Disease Viruses to Allow a Comparative Characterization of the Function of the NS3 Viroporin in Viral Egress. United States Department of Agriculture, September 2013. http://dx.doi.org/10.32747/2013.7699840.bard.

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Project Title: "Development of a plasmid-based reverse genetics system for the Bluetongue and Epizootic Hemorrhagic Disease viruses to allow comparative characterization of the function of the NS3 viroporin in viral egress". Project details: No - IS-4192-09; Participants – Ehrlich M. (Tel Aviv University), Parker J.S. (Cornell University), DermodyT.S. (Vanderbilt University); Period - 2009-2013. Orbiviruses are insect-borne infectious agents of ruminants that cause diseases with considerable economical impact in Israel and the United States. The recent outbreaks of BTV in Europe and of Epizootic Hemorrhagic Disease Virus (EHDV) in Israel, underscore the need for: (i) a better comprehension of the infection process of orbiviruses, (ii) the identification of unique vs. common traits among different orbiviruses, (iii) the development of novel diagnosis and treatment techniques and approaches; all aimed at the achievement of more effective control and treatment measures. It is the context of these broad goals that the present project was carried out. To fulfill our long-term goal of identifying specific viral determinants of virulence, growth, and transmission of the orbiviruses, we proposed to: (i) develop reverse genetics systems for BTV and EHDV2-Ibaraki; and (ii) identify the molecular determinants of the NS3 nonstructural protein related to viroporin/viral egress activities. The first objective was pursued with a two-pronged approach: (i) development of a plasmid-based reverse genetics system for BTV-17, and (ii) development of an "in-vitro" transcription-based reverse genetics system for EHDV2-Ibaraki. Both approaches encountered technical problems that hampered their achievement. However, dissection of the possible causes of the failure to achieve viral spread of EHDV2-Ibaraki, following the transfection of in-vitro transcribed genomic segments of the virus, revealed a novel characteristic of EHDV2-Ibaraki infection: an uncharacteristically low fold increase in titer upon infection of different cell models. To address the function and regulation of NS3 we employed the following approaches: (i) development (together with Anima Cell Metrology) of a novel technique (based on the transfection of fluorescently-labeledtRNAs) that allows for the detection of the levels of synthesis of individual viral proteins (i.e. NS3) in single cells; (ii) development of a siRNA-mediated knockdown approach for the reduction in levels of expression of NS3 in EHDV2-Ibaraki infected cells; (iii) biochemical and microscopy-based analysis of the localization, levels and post-translational modifications of NS3 in infected cells. In addition, we identified the altered regulation and spatial compartmentalization of protein synthesis in cells infected with EHDV2-Ibaraki or the mammalian reovirus. In EHDV2-Ibaraki-infected cells such altered regulation in protein synthesis occurs in the context of a cell stress reponse that includes the induction of apoptosis, autophagy and activation of the stressrelated kinase c-Jun N-terminal Kinase (JNK). Interestingly, inhibition of such stress-related cellular processes diminishes the production of infectious virions, suggesting that EHDV usurps these responses for the benefit of efficient infection. Taken together, while the present project fell short of the generation of novel reverse genetics systems for orbiviruses, the development of novel experimental approaches and techniques, and their employment in the analysis of EHDV-infected cells, yielded novel insights in the interactions of orbiviruses with mammalian cells.

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SRI INTERNATIONAL MENLO PARK CA. LPI845 Liquid Gun Propellant Dermal Toxicity Studies. An Assessment of the LP1846 Utilizing the Mammalian Cell Cytogenetics Assay With Chinese Hamster Ovary (CHO) Cells. Fort Belvoir, VA: Defense Technical Information Center, February 1990. http://dx.doi.org/10.21236/ada238250.

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