Relevant bibliographies by topics / MALDI-TOF MS

Academic literature on the topic 'MALDI-TOF MS'

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Published: 4 June 2021
Last updated: 11 March 2023

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Journal articles on the topic "MALDI-TOF MS"

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NIRASAWA, TAKASHI. "MALDI-TOF-MS." Kagaku To Seibutsu 34, no. 4 (1996): 255–59. http://dx.doi.org/10.1271/kagakutoseibutsu1962.34.255.

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Rim, John Hoon, Yangsoon Lee, Sung Kuk Hong, Yongjung Park, MyungSook Kim, Roshan D’Souza, Eun Suk Park, Dongeun Yong, and Kyungwon Lee. "Insufficient Discriminatory Power of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Dendrograms to Determine the Clonality of Multi-Drug-ResistantAcinetobacter baumanniiIsolates from an Intensive Care Unit." BioMed Research International 2015 (2015): 1–8. http://dx.doi.org/10.1155/2015/535027.

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While pulsed-field gel electrophoresis (PFGE) is recognized as the gold standard method for clonality analysis, MALDI-TOF MS has recently been spotlighted as an alternative tool for species identification. Herein, we compared the dendrograms of multi-drug-resistant (MDR)Acinetobacter baumanniiisolates by using MALDI-TOF MS with those by using PFGE. We used direct colony and protein extraction methods for MALDI-TOF MS dendrograms. The isolates with identical PFGE patterns were grouped into different branches in MALDI-TOF MS dendrograms. Among the isolates that were classified as very close isolates in MALDI-TOF MS dendrogram, PFGE band patterns visually showed complete differences. We numeralized similarity among isolates by measuring distance levels. The Spearman rank correlation coefficient values were 0.449 and 0.297 between MALDI-TOF MS dendrogram using direct colony and protein extraction method versus PFGE, respectively. This study is the first paper focusing solely on the dendrogram function of MALDI-TOF MS compared with PFGE. Although MALDI-TOF MS is a promising tool to identify species in a rapid manner, our results showed that MALDI-TOF MS dendrograms could not substitute PFGE for MDRAcinetobacter baumanniiclonality analysis.
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Chen, Xin-Fei, Xin Hou, Meng Xiao, Li Zhang, Jing-Wei Cheng, Meng-Lan Zhou, Jing-Jing Huang, Jing-Jia Zhang, Ying-Chun Xu, and Po-Ren Hsueh. "Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) Analysis for the Identification of Pathogenic Microorganisms: A Review." Microorganisms 9, no. 7 (July 19, 2021): 1536. http://dx.doi.org/10.3390/microorganisms9071536.

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Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used in the field of clinical microbiology since 2010. Compared with the traditional technique of biochemical identification, MALDI-TOF MS has many advantages, including convenience, speed, accuracy, and low cost. The accuracy and speed of identification using MALDI-TOF MS have been increasing with the development of sample preparation, database enrichment, and algorithm optimization. MALDI-TOF MS has shown promising results in identifying cultured colonies and rapidly detecting samples. MALDI-TOF MS has critical research applications for the rapid detection of highly virulent and drug-resistant pathogens. Here we present a scientific review that evaluates the performance of MALDI-TOF MS in identifying clinical pathogenic microorganisms. MALDI-TOF MS is a promising tool in identifying clinical microorganisms, although some aspects still require improvement.
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Sousa, Roberto B., Keila S. C. Lima, Caleb G. M. Santos, Tanos C. C. França, Eugenie Nepovimova, Kamil Kuca, Marcos R. Dornelas, and Antonio L. S. Lima. "A New Method for Extraction and Analysis of Ricin Samples through MALDI-TOF-MS/MS." Toxins 11, no. 4 (April 3, 2019): 201. http://dx.doi.org/10.3390/toxins11040201.

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We report for the first time the efficient use of accelerated solvent extraction (ASE) for extraction of ricin to analytical purposes, followed by the combined use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and MALDI-TOF MS/MS method. That has provided a fast and unambiguous method of ricin identification for in real cases of forensic investigation of suspected samples. Additionally, MALDI-TOF MS was applied to characterize the presence and the toxic activity of ricin in irradiated samples. Samples containing ricin were subjected to ASE, irradiated with different dosages of gamma radiation, and analyzed by MALDI-TOF MS/MS for verification of the intact protein signal. For identification purposes, samples were previously subjected to SDS-PAGE, for purification and separation of the chains, followed by digestion with trypsin, and analysis by MALDI-TOF MS/MS. The results were confirmed by verification of the amino acid sequences of some selected peptides by MALDI-TOF MS/MS. The samples residual toxic activity was evaluated through incubation with a DNA substrate, to simulate the attack by ricin, followed by MALDI-TOF MS/MS analyses.
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Madhavan, Anitha, Arun Sachu, Nandini Sethuraman, Anil Kumar, and Jayalakshmi Vasudevapanicker. "Evaluation of Matrix-assisted laser desorption/ionization Time-of flight Mass spectrometry (MALDI TOF MS) and VITEK 2 in routine microbial identification." Ghana Medical Journal 55, no. 4 (December 1, 2021): 308–10. http://dx.doi.org/10.4314/gmj.v55i4.12.

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Background: Microbial Identification was done by phenotypic methods. VITEK-2 and Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) are now being increasingly used in laboratories.Objectives: To compare and evaluate the usefulness of MALDI-TOF MS and VITEK-2 in routine microbial identification.Methods: The performances of MALDI-TOF MS and VITEK 2 were compared for identifying microorganisms.Results: MALDI- TOF MS and VITEK-2 correctly identified 96 % (96/100) and 97% (97/100) of the isolates upto the genus level.Conclusion: MALDI TOF MS and VITEK -2 gave comparable identification and error rates. The rapid reduction in turnaround time with MALDI TOF is a significant game-changer in the field of clinical microbiology
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Tan, Zhi Lei, Yu Qiao Wei, Shuang Liang, Ran Zhang, Miao Liu, and Shi Ru Jia. "Rapid Identification of Microorganisms Isolated from Pickled Vegetables by MALDI-TOF Mass Spectrometry." Advanced Materials Research 712-715 (June 2013): 494–97. http://dx.doi.org/10.4028/www.scientific.net/amr.712-715.494.

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Matrix-assisted laser desorption/ionization time-off light mass spectrometry (MALDI-TOF MS) is increasingly used as a microbial diagnostic method for species identification of pathogens. However, MALDI-TOF MS identification of food bacteria was seldom reported. Ten strains isolated from different pickled vegetables were rapid identified by MALDI-TOF MS. The results of MALDI-TOF MS were confirmed by 16S rDNA sequencing method. Different score values in MALDI-TOF MS revealed nineLeuconostoc mesenteroides and oneStaphylococcus.Identification success at the species and genus levels was 90% (9/10) and 100% (10/10), respectively.16S rDNA sequencing results showed that nine stains were identified asLeuconostoc mesenteroides and one wasStaphylococcus saprophyticus.Results obtained demonstrate that MALDI-TOFMS is a promising method for discriminating and identifying food bacteria.
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Mareković, Ivana, Zrinka Bošnjak, Marko Jakopović, Zagorka Boras, Mateja Janković, and Sanja Popović-Grle. "Evaluation of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in Identification of Nontuberculous Mycobacteria." Chemotherapy 61, no. 4 (2016): 167–70. http://dx.doi.org/10.1159/000442517.

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Background/Aims: Species-level identification of nontuberculous mycobacteria (NTM) is important in making decisions about the necessity and choice of antimicrobial treatment. The reason is predictable clinical significance and the susceptibility profile of specific NTM species. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is recognized as a diagnostic tool for routine identification of bacteria and yeasts in the clinical laboratory based on protein fingerprint analysis. The aim of the study was to evaluate MALDI-TOF MS in the identification of NTM. Methods: A total of 25 NTM isolates from liquid cultures were identified with both polymerase chain reaction (PCR)-based hybridization assay and MALDI-TOF MS at the University Hospital Center Zagreb. Results: PCR-based hybridization assay identified 96% (24/25) and MALDI-TOF MS 80% (20/25) of tested NTM isolates. Five isolates with no reliable MALDI-TOF MS identification belonged to the Mycobacterium avium-intracellulare complex. Seventy percent (14/20) of NTM isolates successfully identified with MALDI-TOF MS had a score higher than 2.0, indicating reliable species identification. Conclusion: MALDI-TOF MS is a promising tool for the identification of NTM. With a further improvement of the protein extraction protocol, especially regarding the M. avium-intracellulare complex, MALDI-TOF MS could be an additional standard method for identification of NTM.
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Han, Sang-Soo, Young-Su Jeong, and Sun-Kyung Choi. "Current Scenario and Challenges in the Direct Identification of Microorganisms Using MALDI TOF MS." Microorganisms 9, no. 9 (September 9, 2021): 1917. http://dx.doi.org/10.3390/microorganisms9091917.

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MALDI TOF MS-based microbial identification significantly lowers the operational costs because of minimal requirements of substrates and reagents for extraction. Therefore, it has been widely used in varied applications such as clinical, food, military, and ecological research. However, the MALDI TOF MS method is laced with many challenges including its limitation of the reference spectrum. This review briefly introduces the background of MALDI TOF MS technology, including sample preparation and workflow. We have primarily discussed the application of MALDI TOF MS in the identification of microorganisms. Furthermore, we have discussed the current trends for bioaerosol detection using MALDI TOF MS and the limitations and challenges involved, and finally the approaches to overcome these challenges.
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Oliveira, Thais Cristina de Assis, Maria Aparecida Vasconcelos Paiva Brito, Marcia Giambiagi-de Marval, Nívea Maria Vicentini, and Carla Christine Lange. "Identification of bovine mastitis pathogens using MALDI-TOF mass spectrometry in Brazil." Journal of Dairy Research 88, no. 3 (August 2021): 302–6. http://dx.doi.org/10.1017/s0022029921000595.

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AbstractIn this Research Communication we evaluate the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify 380 bacteria isolated from cases of bovine mastitis in Brazil. MALDI-TOF MS identifications were compared to previous identifications by biochemical tests and 16S rRNA sequencing. MALDI-TOF MS achieved a typeability of 95.5%. The accuracy of MALDI-TOF MS for the identification of Staphylococcus isolates was 93.2%. The agreement between MALDI-TOF MS and biochemical identification of Streptococcus agalactiae was 96%, however, the agreement between these techniques for identifying other catalase-negative, Gram-positive cocci was lower. Agreement in identifying Gram-negative bacteria at the genus level was 90.5%. Our findings corroborate that MALDI-TOF MS is an accurate, rapid and simple technique for identifying bovine mastitis pathogens. The availability of this methodology in some research institutions would represent a significant step toward increasing the diagnosis and epidemiological studies of bovine mastitis and other animal infectious diseases in Brazil.
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Schiller, Jurgen. "MALDI-TOF MS in lipidomics." Frontiers in Bioscience 12, no. 1 (2007): 2568. http://dx.doi.org/10.2741/2255.

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Dissertations / Theses on the topic "MALDI-TOF MS"

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Leander, Ellinor. "Artidentifiering av mögelsvamp med MALDI-TOF MS." Thesis, Linnéuniversitetet, Institutionen för kemi och biomedicin (KOB), 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-80166.

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Snabb och korrekt artidentifiering är avgörande för effektiv behandling av svampinfektioner, särskilt bland immunsupprimerade patienter. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) används rutinmässigt på kliniska laboratorier för identifiering av karaktäristiska proteinmönster hos bakterier och jästsvampar genom tolkning av proteinspektra i en masspektradatabas för korrekt artidentifiering. Mögelsvamparnas hårda cellvägg och heterogena växtsätt med varierande proteinuttryck beroende på mognadsstadie, försvårar identifiering med MALDI-TOF MS. Metodens tänkbara fördelar mot traditionella metoden mikroskopering är förkortade svarstider, säkrare artidentifiering av fler arter och mindre beroende av subjektiv morfologisk bedömning. Studiens syfte var att undersöka om MALDI-TOF MS kunde anpassas och användas för identifieringen av mögelsvamp i klinisk rutindiagnostik. Fyra referensstammar (Aspergillus niger, A. fumigatus, A.terreus, A.flavus) och ett kliniskt isolat (A.terreus) undersöktes. Preparationsmetoderna (I) fullständig myrsyraextraktion, (II) direktapplicering och (III) suspension i destillerat vatten användes för analys av sporer och frontmycel hos yngre och äldre mögelkulturer. Två olika masspektradatabaser för artidentifiering jämfördes; rutindatabasen BDAL och den specialiserade mögeldatabasen Filamentous Fungi Library. Även plocktekniken av mögelmaterial inför analys med MALDI-TOF MS utvärderades. Vid vissa tillfällen förbättrades artidentifieringen efter extraktion av mögelkulturerna, medan i andra fall var direktapplicering fullt tillräcklig. Mögelmaterial med mycket sporer tenderade ge något fler artidentifieringar i BDAL oavsett kulturernas ålder.  Filamentous Fungi Library tenderade i vissa fall ge bättre resultat jämfört med BDAL för yngre kulturer. Fler studier krävs för att utvärdera och optimera MALDI-TOF MS som metod för artidentifiering av mögelsvamp.
Rapid and accurate species identification is crucial for successful treatment of fungal infections, especially among immunosuppressed patients. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is used routinely at clinical laboratories to identify characteristic protein patterns of bacteria and yeast by the interpretation of protein spectra in a database for accurate species identification. The hard cell wall of the mold and the heterogeneous growth with varying protein expression due to maturation, complicates identification with MALDI-TOF MS. The potential benefits of this method compared to microscopy as traditional method are shortened turn-around times, safer species identification of more species that is independent on subjective morphological assessment. The purpose of the study was to investigate whether MALDI-TOF MS could be adapted and used for the identification of molds in clinical routine diagnostics. Four reference strains (Aspergillus niger, A.fumigatus, A.terreus, A.flavus) and a clinical isolate (A.terreus) were examined. The preparation methods (I) complete formic acid extraction, (II) direct application and (III) suspension in distilled water were used for analysis of spores and frontmycelium from younger and older mold cultures. Two different masspektradatabases for species identification were compared; routine database BDAL and the specialized mold database, Filamentous Fungi Library. Also the collecting technique of mold prior to analysis with MALDI-TOF MS was evaluated. Sometimes, the species identification improved after extraction of mold cultures, while in other cases direct application was sufficient. Cultures with a lot of spores tended to give slightly more species identifications in BDAL regardless of the age of cultures. Filamentous Fungi Library, in some cases, tended to improve the performance compared to BDAL for younger cultures. More studies are required to evaluate and optimize MALDI-TOF MS as a method of mold identification.
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Kempka, Martin. "Improved mass accuracy in MALDI-TOF-MS analysis." Licentiate thesis, Stockholm : Division of Analytical Chemistry, Royal Institute of Technology, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-313.

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Halgunset, Anders. "Typing av Legionella pneumophila med MALDI-TOF MS." Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for bioteknologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-24697.

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Legionella pneumophila er fakultativ intracellulære bakterier som lever naturlig i akvatiske habitater. De kan replikere i makrofager og protozoer, bli overført til mennesker gjennom areosoler og føre til smitte. Ved eventuelle utbrudd er det viktig å kunne identifisere kilden, noe som i dag bli gjort ved å typebestemme L. pneumophila med sekvensbasert typing (SBT). Samtidig har matrix-assisted laser desorption/ionization ? time-of-flight (MALDI?TOF) mass spectrometry (MS) vist seg å kunne brukes til rask og effektiv identifisering av en rekke mikroorganismer på artsnivå, deriblant Legionella. Hos enkelte andre mikroorganismer har MALDI?TOF MS også vist å kunne skille mellom underarter. MALDI?TOF MS ble i denne oppgaven benyttet for å undersøke om det var mulig å skille L. pneumophila fra hverandre på stammenivå ved hjelp av MALDI Biotyper 3.0 programvare. Standardprotokollen anbefalt av Bruker Daltonics for proteinekstraksjon fra mikroorganismer ble tilpasset/optimalisert for bruk på L. pneumophila, slik at reproduserbare massespektre ble oppnådd. Evalueringen viste at proteinekstraksjon fra ca 2 µl biologisk materiale med 10 µl acetonnitrill og 10 µl maursyre, ga best scoreverdi opp mot Bruker Daltonics referansebibliotek. Den optimale temperaturen og varigheten for dyrkning ble vist å være henholdsvis 37 ˚C og 48 ± 2 timer. Det ble vist at lagring av skåler/kolonier med L. pneumophila ved 37 ˚C førte til forandringer i massespektrene, mens lagring ved 20 ˚C ikke medførte slike forandringer og at stabile massespektre ble oppnådd etter seks dagers lagring.Det ble bygget opp et referansebibliotek i MALDI Biotyper 3.0 bestående av referansespekter/referansetopplister (MSP) fra til sammen 48 Legionella spp. hvorav 41 var L. pneumophila -isolater. Med referansebiblioteket ble det vist at MSP (dannet ved standard innstillinger) for mange L. pneumophila var for like hverandre til å gi identifisering mot egne MSP. En klyngeanalyse ble brukt i sammenligning av L. pneumophila mellom SBT og MSP fra referansebiblioteket dannet med MALDI?TOF MS. Sammenligningen viste at diversiteten i proteinsammensetningen hos L. pneumophila, representert som MSP, ikke ga samme oppløselighet som gjennom sekvensvariasjonene fra genene i SBT -analysen.
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Bertilsson, Sarah. "Glycanmapping of glycoproteins with UPLC-FLR-MALDI/TOF-MS." Thesis, Uppsala universitet, Analytisk kemi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-228040.

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Dempwolf, Wibke. "MALDI-TOF in der kontrollierten radikalischen Polymerisation und Präpolymeranalyse." Clausthal-Zellerfeld Papierflieger, 2007. http://d-nb.info/990376834/04.

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Worster, Belinda Mary. "MALDI-TOF-MS and neuropeptide signalling in the nervous system." Thesis, University of Sussex, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360682.

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Niare, Sirama. "Identification du repas sanguin des moustiques par MALDI-TOF MS." Thesis, Aix-Marseille, 2017. http://www.theses.fr/2017AIXM0575/document.

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Le MALDI-TOF MS (Matrix Assisted, Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry) est une technique protéomique qui est utilisée en routine pour l’identification des microorganismes dans les laboratoires de microbiologie. Ainsi, dans ce travail nous avons évalué le MALDI-TOF MS pour l’identification du repas sanguin des moustiques. Dans la première partie de notre travail, une revue bibliographique a été effectuée sur les différentes méthodes (sérologiques, biologie moléculaire) connues dans les études de préférence trophiques des arthropodes. La deuxième partie fut l’optimisation du MALDI-TOF MS pour l’identification de l’origine du repas sanguin des moustiques. Pour l’optimisation, Anopheles gambiae Giles et Aedes albopictus ont été artificiellement nourris sur le sang de plusieurs hôtes vertébrés en utilisant l’appareil Hemotek durant deux heures sous les conditions standard. Nos résultats ont montré que la comparaison des spectres provenant des moustiques nourris sur le même type de sang révèle une grande reproductibilité des profils protéiques. L’interrogation des MS spectres contre la base de données a révélé une identification correcte de l'origine du repas sanguin pour les spécimens collectés moins de 24 heures après la prise du repas sanguin. Pour les échantillons collectés sur le terrain, le MALDI-TOF MS a permis de détecter dans le repas de sang des moustiques une grande diversité d’hôtes domestiques. En conséquence la technique MALDI-TOF MS serait un outil efficace pour les études de surveillance épidémiologique des maladies vectorielles et l'identification de la préférence trophique de spécimens fraichement gorgés
MALDI-TOF MS (Matrix Assisted, Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry) is a proteomic technique that routinely used for microorganisms identification in clinical microbiology laboratory. Recently, the MALDI-TOF MS was successfully used as a innovative tool for arthropod identification. Thus, in this work we evaluated the MALDI-TOF MS to identify the blood meal sources from engorged mosquitoes. In the first part of our work, a bibliographical review was carried out on the different methods (serological, molecular biology) known in the trophic preference determination of hematophagous arthropods. The second part was optimization of the MALDI-TOF MS for identifying the origin of the blood meal of mosquitoes. For optimization, the Anopheles gambiae Giles and Aedes albopictus were artificially fed on several vertebrate hosts blood using the Hemotek device for two hours under standard conditions. Our results showed intra-species reproducibility and inter-species specificity of MS spectra from mosquitoes engorged on the same or different vertebrate hosts. The MS spectra querying against the database reveal a correct identification of the the blood meal origin from the specimens collected less than 24 hours post-feeding. For field samples, MALDI-TOF MS allowed to detect the mosquitoes blood meal fed on wide variety of domestic hosts. Consequently the MALDI-TOF MS technique would be an effective tool for epidemiological surveys of vector-borne diseases and the identification of the trophic preference of mosquito freshly engorged
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Dallacker-Losensky, Kevin. "Identifizierung von obligaten Anaerobiern der Bacteroides fragilis Gruppe einschließlich Metronidazol-resistenter und Enterotoxin-positiver Stämme mittels MALDI-TOF MS." Doctoral thesis, Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-206555.

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Die klassische Identifizierung von obligat anaeroben Bakterien ist mit einem hohen Labor- und Zeitaufwand verbunden. Um festzustellen, ob die Identifizierung mittels Matrix-unterstützter Laser-Desorption/Ionisation und Massenspektrometrie mit Flugzeitanalysator (Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; MALDI-TOF MS) ein Verfahren ist, um obligate Anaerobier eindeutig zu identifizieren, wurde mit der vorliegenden Arbeit die Identifizierung von unterschiedlichen Spezies der B. fragilis Gruppe mittels MALDI-TOF MS untersucht. Hierfür wurden 105 obligate Anaerobier der B. fragilis Gruppe aus der Stammsammlung des Institutes für Medizinische Mikrobiologie und Infektionsepidemiologie der Universität Leipzig untersucht. Es fanden sich für die untersuchten Erreger Spektren mit sehr guter Auflösung. Eine Identifizierung und Differenzierung war eindeutig möglich. Unter Verwendung dieser Daten wurde eine Referenzdatenbank erstellt. Die erhaltenen Ergebnisse wurden mittels einer verblindeten Studie überprüft, wobei 52 von 53 (98,1%) der untersuchten Stämme eindeutig identifiziert werden konnten. Dies schließt ebenfalls die Identifizierung und Differenzierung von 15 Metronidazol-sensiblen/ Enterotoxin-negativen, 8 Metronidazol-resistenten/ Enterotoxin-negativen und 8 Metronidazol-sensiblen/ Enterotoxin-positiven B. fragilis Stämmen ein. Die Identifizierung mittels MALDI-TOF MS ist somit eine zuverlässige Methode zur Identifizierung von obligaten Anaerobiern der B. fragilis Gruppe. Weiterhin finden sich Hinweise, dass ein Nachweis von Resistenz-, Virulenz- und Pathogenitätsfaktoren mittels MALDI-TOF MS bei diesen Erregern möglich ist.
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Chen, Shuo. "MALDI-TOF MS data processing using wavelets, splines and clustering techniques." [Johnson City, Tenn. : East Tennessee State University], 2004. http://etd-submit.etsu.edu/etd/theses/available/etd-1112104-113123/unrestricted/ChenS121404f.pdf.

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Thesis (M.S.)--East Tennessee State University, 2004.
Title from electronic submission form. ETSU ETD database URN: etd-1112104-113123 Includes bibliographical references. Also available via Internet at the UMI web site.
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Hamdi, Cassandra. "Clostridium difficile : Rapid typing Clostridium difficile using MALDI-TOF MS analysis." Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17659.

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Books on the topic "MALDI-TOF MS"

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Hosseini, Samira, and Sergio O. Martinez-Chapa. Fundamentals of MALDI-ToF-MS Analysis. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-2356-9.

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Shah, Haroun N., and Saheer E. Gharbia, eds. MALDI-TOF and Tandem MS for Clinical Microbiology. Chichester, UK: John Wiley & Sons, Ltd, 2017. http://dx.doi.org/10.1002/9781118960226.

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Shah, Haroun N., and Saheer E. Gharbia. MALDI-TOF and Tandem MS for Clinical Microbiology. Wiley & Sons, Incorporated, John, 2017.

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Shah, Haroun N., and Saheer E. Gharbia. MALDI-TOF and Tandem MS for Clinical Microbiology. Wiley & Sons, Limited, John, 2017.

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Shah, Haroun N., and Saheer E. Gharbia. MALDI-TOF and Tandem MS for Clinical Microbiology. Wiley & Sons, Incorporated, John, 2017.

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Becker, Karsten, and Sören Schubert, eds. MALDI-TOF MS Application for Susceptibility Testing of Microorganisms. Frontiers Media SA, 2020. http://dx.doi.org/10.3389/978-2-88966-313-2.

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Hosseini, Samira, and Sergio O. Martinez-Chapa. Fundamentals of MALDI-ToF-MS Analysis: Applications in Bio-diagnosis, Tissue Engineering and Drug Delivery. Springer, 2016.

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Book chapters on the topic "MALDI-TOF MS"

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Mehlhorn, Heinz. "MALDI-TOF MS." In Encyclopedia of Parasitology, 1575. Berlin, Heidelberg: Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-662-43978-4_4742.

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Mehlhorn, Heinz. "MALDI-TOF MS." In Encyclopedia of Parasitology, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-642-27769-6_4742-1.

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Santos, Cledir, Paula Galeano, Reginaldo Lima Neto, Manoel Marques Evangelista Oliveira, and Nelson Lima. "MALDI-TOF MS and its requirements for fungal identification." In Trends in the systematics of bacteria and fungi, 119–40. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789244984.0119.

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Abstract Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is now used as a routine technique for the fast and reliable identification of fungi at the species level and, currently, it represents an important phenotypic methodology based on proteomic profiles. The main limitations to MALDI-TOF MS for fungal identification are related to sample quality (e.g. quality of biological material such as rigidity or pigmentation of cell walls), sample preparation (e.g. the myriad of sample preparation methodologies that deliver different data sets to different MALDI-TOF MS databases) and the databases themselves (e.g. the 'black-box' commercial databases). This chapter presents an overview and discussion of the use of MALDI-TOF MS for fungal identification. The major known limitations of the technique for fungal taxonomy, and how to overcome these, are also discussed.
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Pham, Thang V., and Connie R. Jimenez. "Computational Analysis of High-Throughput MALDI-TOF-MS-Based Peptide Profiling." In MALDI MS, 411–30. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2013. http://dx.doi.org/10.1002/9783527335961.ch10.

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Guo, Qingbin, Lianzhong Ai, and Steve W. Cui. "MALDI-TOF-MS for Polysaccharides Analysis." In SpringerBriefs in Molecular Science, 65–68. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-96370-9_8.

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Liyanage, Rohana, and Jackson O. Lay. "An Introduction to MALDI-TOF MS." In Identification of Microorganisms by Mass Spectrometry, 39–60. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/0471748641.ch3.

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Mandal, Santi M., and Debarati Paul. "MALDI-TOF MS for Bacterial Identification." In Automation and Basic Techniques in Medical Microbiology, 77–86. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2372-5_6.

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Hosseini, Samira, and Sergio O. Martinez-Chapa. "Principles and Mechanism of MALDI-ToF-MS Analysis." In Fundamentals of MALDI-ToF-MS Analysis, 1–19. Singapore: Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-10-2356-9_1.

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Hosseini, Samira, and Sergio O. Martinez-Chapa. "Fundamentals of Biosensors and Application of MALDI-ToF-MS in Bio-diagnostic Domain." In Fundamentals of MALDI-ToF-MS Analysis, 21–39. Singapore: Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-10-2356-9_2.

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Hosseini, Samira, and Sergio O. Martinez-Chapa. "Fundamentals of Tissue Engineering and Application of MALDI-ToF-MS in Analysis of the Scaffold Materials." In Fundamentals of MALDI-ToF-MS Analysis, 41–52. Singapore: Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-10-2356-9_3.

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Conference papers on the topic "MALDI-TOF MS"

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Lopez-Cortes, Xaviera A., Cesar A. Astudillo, Camila Gonzalez, and Sebastian Maldonado. "Semi-supervised learning for MS MALDI-TOF data." In 2021 IEEE Latin American Conference on Computational Intelligence (LA-CCI). IEEE, 2021. http://dx.doi.org/10.1109/la-cci48322.2021.9769825.

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Rusnáková, Lenka, and Josef Havel. "Nano-gold applications in MALDI TOF MS of peptides." In XIIth Conference Biologically Active Peptides. Prague: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2011. http://dx.doi.org/10.1135/css201113120.

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Jianqiu Zhang, Honghui Wang, Anthony Suffredini, Denise Gonzales, Elias Gonzalez, Yufei Huang, and Xiaobo Zhou. "Bayesian peak detection for Pro-TOF MS MALDI data." In ICASSP 2008 - 2008 IEEE International Conference on Acoustics, Speech and Signal Processing. IEEE, 2008. http://dx.doi.org/10.1109/icassp.2008.4517696.

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Kornfeld, Rich, James W. Kenny, Scot R. Weinberger, Yi Yang, and Ron Orlando. "Glycoprotein analysis using enzymatic digestion and MALDI-TOF MS." In Photonics West '96, edited by Gerald E. Cohn, Steven A. Soper, and C. H. Winston Chen. SPIE, 1996. http://dx.doi.org/10.1117/12.237629.

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Huilong Yang, Baoyou Liu, Yuyou Wang, and Limei Han. "MALDI-TOF MS progress and its application in Environmental Science." In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5965161.

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Калинин, А. В., Е. А. Котенева, and О. И. Цыганкова. "Идентификация и дискриминация бактерий рода Bacillus методом MALDI-TOF MS." In VI Пущинская школа-конференция «Биохимия, физиология и биосферная роль микроорганизмов». ИД «Вода: химия и экология», 2019. http://dx.doi.org/10.18334/ibpm2019.25-27.

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Калинин, А. В., Е. А. Котенева, and О. И. Цыганкова. "Идентификация и дискриминация бактерий рода Bacillus методом MALDI-TOF MS." In VI Пущинская школа-конференция «Биохимия, физиология и биосферная роль микроорганизмов». ИД «Вода: химия и экология», 2019. http://dx.doi.org/10.18334/ibpm2019_25-27.

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Erhard, M., M. Metzner, D. Köhler-Repp, B. Köhler, and R. Storandt. "Infektionserreger beim Schwein – Analyse des kultivierbaren Mikrobioms mittels MALDI-TOF MS." In 20. Workshop des Arbeitskreises ‚Respiratorisches System‘ der Deutschen Veterinärmedizinischen Gesellschaft (DVG) in Kooperation mit der Deutschen Gesellschaft für Pneumologie und Beatmungsmedizin (DPG). Georg Thieme Verlag KG, 2017. http://dx.doi.org/10.1055/s-0037-1602728.

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Havlova´, Va´clava, Radek Cˇervinka, Ulrich Noseck, Thomas Brasser, and Josef Havel. "The Ruprechtov Natural Analogue Site (CZ) Study: Mobile Natural Organic Matter Identification, Characterisation and Link to PA Relevant Processes." In ASME 2009 12th International Conference on Environmental Remediation and Radioactive Waste Management. ASMEDC, 2009. http://dx.doi.org/10.1115/icem2009-16341.

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The Ruprechtov Natural Analogue (CZ) Programme has been focused on studying real system processes, relevant to performance assessment (PA) of sediment formations that can form the overburden of geological repository host rocks. The site has been extensively studied due to its geological constitution (granite – kaolin – clay – U mineralisation – organic matter). The presented study used Ruprechtov unique but well-described geological conditions in order to identify and characterise mobile organic matter (MOM) that can be easily released into groundwater and can influence PA relevant specie migration due to complexation/sorption reaction. The modern analytical method MALDI-TOF MS was used for characterisation. It was found that only a small fraction of sedimentary natural organic matter (NOM) from the site was easily releasable (max. 5%) as MOM, resulting in low organic substance concentration in natural groundwater. MOM amount released was decreasing with increasing NOM content. MALDI-TOF MS proved to be a useful tool to characterize organic substances, either natural ones or artificially released from natural organic matter samples. A noticeable fingerprint for all the MOM compounds analysed was found at MALDI-TOF MS spectra. This showed that MOM from the Ruprechtov site was in all cases composed of molecules with low molecular weight (under 1000 Da). As determined by the consequent geochemical analyses, despite groundwater reducing conditions MOM compounds would be mainly interacting with U(VT) in the groundwater, being present as more abundant U specie. Good correspondence of results enabled to consider the extracted humic acid HA 12/3 as a mobile organic matter fraction representative.
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Huang, Hung-Hsiang, Heng-Huan Lee, Pei-Lun Tsai, and Kay-Hooi Khoo. "DISTINCTIVE FEATURES OF MALDI-Q-TOF MS AND MS/MS ANALYSIS OF PERMETHYLATED GLYCANS FOR FACILE GLYCOMIC SEQUENCING." In XXIst International Carbohydrate Symposium 2002. TheScientificWorld Ltd, 2002. http://dx.doi.org/10.1100/tsw.2002.398.

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Reports on the topic "MALDI-TOF MS"

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Blumwald, Eduardo, and Avi Sadka. Citric acid metabolism and mobilization in citrus fruit. United States Department of Agriculture, October 2007. http://dx.doi.org/10.32747/2007.7587732.bard.

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Accumulation of citric acid is a major determinant of maturity and fruit quality in citrus. Many citrus varieties accumulate citric acid in concentrations that exceed market desires, reducing grower income and consumer satisfaction. Citrate is accumulated in the vacuole of the juice sac cell, a process that requires both metabolic changes and transport across cellular membranes, in particular, the mitochondrial and the vacuolar (tonoplast) membranes. Although the accumulation of citrate in the vacuoles of juice cells has been clearly demonstrated, the mechanisms for vacuolar citrate homeostasis and the components controlling citrate metabolism and transport are still unknown. Previous results in the PIs’ laboratories have indicated that the expression of a large number of a large number of proteins is enhanced during fruit development, and that the regulation of sugar and acid content in fruits is correlated with the differential expression of a large number of proteins that could play significant roles in fruit acid accumulation and/or regulation of acid content. The objectives of this proposal are: i) the characterization of transporters that mediate the transport of citrate and determine their role in uptake/retrieval in juice sac cells; ii) the study of citric acid metabolism, in particular the effect of arsenical compounds affecting citric acid levels and mobilization; and iii) the development of a citrus fruit proteomics platform to identify and characterize key processes associated with fruit development in general and sugar and acid accumulation in particular. The understanding of the cellular processes that determine the citrate content in citrus fruits will contribute to the development of tools aimed at the enhancement of citrus fruit quality. Our efforts resulted in the identification, cloning and characterization of CsCit1 (Citrus sinensis citrate transporter 1) from Navel oranges (Citrus sinesins cv Washington). Higher levels of CsCit1 transcripts were detected at later stages of fruit development that coincided with the decrease in the juice cell citrate concentrations (Shimada et al., 2006). Our functional analysis revealed that CsCit1 mediates the vacuolar efflux of citrate and that the CsCit1 operates as an electroneutral 1CitrateH2-/2H+ symporter. Our results supported the notion that it is the low permeable citrateH2 - the anion that establishes the buffer capacity of the fruit and determines its overall acidity. On the other hand, it is the more permeable form, CitrateH2-, which is being exported into the cytosol during maturation and controls the citrate catabolism in the juice cells. Our Mass-Spectrometry-based proteomics efforts (using MALDI-TOF-TOF and LC2- MS-MS) identified a large number of fruit juice sac cell proteins and established comparisons of protein synthesis patterns during fruit development. So far, we have identified over 1,500 fruit specific proteins that play roles in sugar metabolism, citric acid cycle, signaling, transport, processing, etc., and organized these proteins into 84 known biosynthetic pathways (Katz et al. 2007). This data is now being integrated in a public database and will serve as a valuable tool for the scientific community in general and fruit scientists in particular. Using molecular, biochemical and physiological approaches we have identified factors affecting the activity of aconitase, which catalyze the first step of citrate catabolism (Shlizerman et al., 2007). Iron limitation specifically reduced the activity of the cytosolic, but not the mitochondrial, aconitase, increasing the acid level in the fruit. Citramalate (a natural compound in the juice) also inhibits the activity of aconitase, and it plays a major role in acid accumulation during the first half of fruit development. On the other hand, arsenite induced increased levels of aconitase, decreasing fruit acidity. We have initiated studies aimed at the identification of the citramalate biosynthetic pathway and the role(s) of isopropylmalate synthase in this pathway. These studies, especially those involved aconitase inhibition by citramalate, are aimed at the development of tools to control fruit acidity, particularly in those cases where acid level declines below the desired threshold. Our work has significant implications both scientifically and practically and is directly aimed at the improvement of fruit quality through the improvement of existing pre- and post-harvest fruit treatments.
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