Relevant bibliographies by topics / Malate transport

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Malate transport'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Malate transport"

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Osothsilp, C., and R. E. Subden. "Malate transport in Schizosaccharomyces pombe." Journal of Bacteriology 168, no. 3 (1986): 1439–43. http://dx.doi.org/10.1128/jb.168.3.1439-1443.1986.

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Agbanyo, F. R., G. Moses, and N. F. Taylor. "L-Malate transport and proton symport in vesicles prepared from Pseudomonas putida." Biochemistry and Cell Biology 64, no. 11 (November 1, 1986): 1190–94. http://dx.doi.org/10.1139/o86-156.

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In vesicles from glucose-grown Pseudomonas putida, L-malate is transported by nonspecific physical diffusion. L-Malate also acts as an electron donor and generates a proton motive force (Δp) of 129 mV which is composed of a membrane potential (Δψ) of 60 mV and a ΔpH of 69 mV. In contrast, vesicles from succinate-grown cells (a) transport L-malate by a carrier-mediated system with a Km value of 14.3 mM and a Vmax of 313 nmol∙mg protein−1∙min−1, (b) generate no Δψ, ΔpH, or Δp when L-malate is the electron donor, and (c) produce an extravesicular alkaline pH during the transport of L-malate. A kinetic analysis of this L-malate-induced proton transport gives a Km value of 16 mM and a Vmax of 667 nmol H+∙mg protein−1∙min−1. This corresponds to a H+/L-malate ratio of 2.1. The failure to generate a Δp in these vesicles is considered, therefore, to be consistent with the induction in succinate-grown cells of an electrogenic proton symport L-malate transport system.
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Günzel, Dorothee, Karin Hintz, Simone Durry, and Wolf-Rüdiger Schlue. "Mg2+-Malate Co-Transport, a Mechanism for Na+-Independent Mg2+ Transport in Neurons of the Leech Hirudo medicinalis." Journal of Neurophysiology 94, no. 1 (July 2005): 441–53. http://dx.doi.org/10.1152/jn.01221.2004.

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Mg2+-extrusion from Mg2+-loaded neurons of the leech, Hirudo medicinalis, is mediated mainly by Na+/Mg2+ antiport. However, in a number of leech neurons, Mg2+ is extruded in the nominal absence of extracellular Na+, indicating the existence of an additional, Na+-independent Mg2+ transport mechanism. This mechanism was investigated using electrophysiological and microfluorimetrical techniques. The rate of Na+-independent Mg2+ extrusion from Mg2+-loaded leech neurons was found to be independent of extracellular Ca2+, K+, NO3−, HCO3−, SO42−, HPO42−, and of intra- and extracellular pH. Na+-independent Mg2+ extrusion was not inhibited by 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS), furosemide, ouabain, vanadate, iodoacetate, 4-amino-hippurate, or α-cyano-4-hydroxycinnamate and was not influenced by changes in the membrane potential in voltage-clamp experiments. Na+-independent Mg2+ extrusion was, however, inhibited by the application of 2 mM probenecid, a blocker of organic anion transporters, suggesting that Mg2+ might be co-transported with organic anions. Extracellularly, of all organic anions tested (malate, citrate, lactate, α-ketoglutarate, and 4-amino-hippurate) only high, but physiological, concentrations of malate (30 mM) had a significant inhibitory effect on Na+-independent Mg2+ extrusion. Intracellularly, iontophoretically injected malate, citrate, or fura-2, but not Cl−, α-ketoglutarate, glutamate, succinate, or urate, were stimulating Na+-independent Mg2+ extrusion from those neurons that initially did not extrude Mg2+ in Na+-free solutions. Our data indicate that Mg2+ is co-transported with organic anions, preferably with malate, the predominant extracellular anion in the leech. The proposed model implies that, under experimental conditions, malate drives Mg2+ extrusion, whereas under physiological conditions, malate is actively taken up, driven by Mg2+, so that malate can be metabolized.
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Pallotta, Maria Luigia, Alessandra Fratianni, and Salvatore Passarella. "Metabolite transport in isolated yeast mitochondria: fumarate/malate and succinate/malate antiports." FEBS Letters 462, no. 3 (November 30, 1999): 313–16. http://dx.doi.org/10.1016/s0014-5793(99)01535-5.

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Krom, Bastiaan P., Ronald Aardema, and Juke S. Lolkema. "Bacillus subtilis YxkJ Is a Secondary Transporter of the 2-Hydroxycarboxylate Transporter Family That Transports l-Malate and Citrate." Journal of Bacteriology 183, no. 20 (October 15, 2001): 5862–69. http://dx.doi.org/10.1128/jb.183.20.5862-5869.2001.

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ABSTRACT The genome of Bacillus subtilis contains two genes that code for membrane proteins that belong to the 2-hydroxycarboxylate transporter family. Here we report the functional characterization of one of the two, yxkJ, which codes for a transporter protein named CimHbs. The gene was cloned and expressed inEscherichia coli and complemented the citrate-negative phenotype of wild-type E. coli and the malate-negative phenotype of the E. coli strain JRG4008, which is defective in malate uptake. Subsequent uptake studies in whole cells expressing CimHbs clearly demonstrated the citrate and malate transport activity of the protein. Immunoblot analysis showed that CimHbs is a 48-kDa protein that is well expressed in E. coli. Studies with right-side-out membrane vesicles demonstrated that CimHbs is an electroneutral proton-solute symporter. No indications were found for the involvement of Na+ ions in the transport process. Inhibition of the uptake catalyzed by CimHbs by divalent metal ions, together with the lack of effect on transport by the chelator EDTA, showed that CimHbs translocates the free citrate and malate anions. Among a large set of substrates tested, only malate, citramalate, and citrate competitively inhibited citrate transport catalyzed by CimHbs. The transporter is strictly stereoselective, recognizing only the S enantiomers of malate and citramalate. Remarkably, though citramalate binds to the transporter, it is not translocated.
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Zhang, Lihua, Baiquan Ma, Changzhi Wang, Xingyu Chen, Yong-Ling Ruan, Yangyang Yuan, Fengwang Ma, and Mingjun Li. "MdWRKY126 modulates malate accumulation in apple fruit by regulating cytosolic malate dehydrogenase (MdMDH5)." Plant Physiology 188, no. 4 (January 25, 2022): 2059–72. http://dx.doi.org/10.1093/plphys/kiac023.

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Abstract The content of organic acids greatly influences the taste and storage life of fleshy fruit. Our current understanding of the molecular mechanism of organic acid accumulation in apple (Malus domestica) fruit focuses on the aluminum-activated malate transporter 9/Ma1 gene. In this study, we identified a candidate gene, MdWRKY126, for controlling fruit acidity independent of Ma1 using homozygous recessive mutants of Ma1, namely Belle de Boskoop “BSKP” and Aifeng “AF.” Analyses of transgenic apple calli and flesh and tomato (Solanum lycopersicum) fruit demonstrated that MdWRKY126 was substantially associated with malate content. MdWRKY126 was directly bound to the promoter of the cytoplasmic NAD-dependent malate dehydrogenase MdMDH5 and promoted its expression, thereby enhancing the malate content of apple fruit. In MdWRKY126 overexpressing calli, the mRNA levels of malate-associated transporters and proton pump genes also significantly increased, which contributed to the transport of malate accumulated in the cytoplasm to the vacuole. These findings demonstrated that MdWRKY126 regulates malate anabolism in the cytoplasm and coordinates the transport between cytoplasm and vacuole to regulate malate accumulation. Our study provides useful information to improve our understanding of the complex mechanism regulating apple fruit acidity.
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Zoglowek, Cornelia, Silke Krömer, and Hans W. Heldt. "Oxaloacetate and Malate Transport by Plant Mitochondria." Plant Physiology 87, no. 1 (May 1, 1988): 109–15. http://dx.doi.org/10.1104/pp.87.1.109.

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Wang, Yuqi, Ruihong Li, Demou Li, Xiaomin Jia, Dangwei Zhou, Jianyong Li, Sangbom M. Lyi, et al. "NIP1;2 is a plasma membrane-localized transporter mediating aluminum uptake, translocation, and tolerance in Arabidopsis." Proceedings of the National Academy of Sciences 114, no. 19 (April 24, 2017): 5047–52. http://dx.doi.org/10.1073/pnas.1618557114.

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Members of the aquaporin (AQP) family have been suggested to transport aluminum (Al) in plants; however, the Al form transported by AQPs and the roles of AQPs in Al tolerance remain elusive. Here we report that NIP1;2, a plasma membrane-localized member of the Arabidopsis nodulin 26-like intrinsic protein (NIP) subfamily of the AQP family, facilitates Al-malate transport from the root cell wall into the root symplasm, with subsequent Al xylem loading and root-to-shoot translocation, which are critical steps in an internal Al tolerance mechanism in Arabidopsis. We found that NIP1;2 transcripts are expressed mainly in the root tips, and that this expression is enhanced by Al but not by other metal stresses. Mutations in NIP1;2 lead to hyperaccumulation of toxic Al3+ in the root cell wall, inhibition of root-to-shoot Al translocation, and a significant reduction in Al tolerance. NIP1;2 facilitates the transport of Al-malate, but not Al3+ ions, in both yeast and Arabidopsis. We demonstrate that the formation of the Al-malate complex in the root tip apoplast is a prerequisite for NIP1;2-mediated Al removal from the root cell wall, and that this requires a functional root malate exudation system mediated by the Al-activated malate transporter, ALMT1. Taken together, these findings reveal a critical linkage between the previously identified Al exclusion mechanism based on root malate release and an internal Al tolerance mechanism identified here through the coordinated function of NIP1;2 and ALMT1, which is required for Al removal from the root cell wall, root-to-shoot Al translocation, and overall Al tolerance in Arabidopsis.
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Ramesh, Sunita A., Muhammad Kamran, Wendy Sullivan, Larissa Chirkova, Mamoru Okamoto, Fien Degryse, Michael McLaughlin, Matthew Gilliham, and Stephen D. Tyerman. "Aluminum-Activated Malate Transporters Can Facilitate GABA Transport." Plant Cell 30, no. 5 (April 4, 2018): 1147–64. http://dx.doi.org/10.1105/tpc.17.00864.

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Waters, James K., Thomas P. Mawhinney, and David W. Emerich. "Nitrogen Assimilation and Transport by Ex Planta Nitrogen-Fixing Bradyrhizobium diazoefficiens Bacteroids Is Modulated by Oxygen, Bacteroid Density and l-Malate." International Journal of Molecular Sciences 21, no. 20 (October 13, 2020): 7542. http://dx.doi.org/10.3390/ijms21207542.

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Symbiotic nitrogen fixation requires the transfer of fixed organic nitrogen compounds from the symbiotic bacteria to a host plant, yet the chemical nature of the compounds is in question. Bradyrhizobium diazoefficiens bacteroids were isolated anaerobically from soybean nodules and assayed at varying densities, varying partial pressures of oxygen, and varying levels of l-malate. Ammonium was released at low bacteroid densities and high partial pressures of oxygen, but was apparently taken up at high bacteroid densities and low partial pressures of oxygen in the presence of l-malate; these later conditions were optimal for amino acid excretion. The ratio of partial pressure of oxygen/bacteroid density of apparent ammonium uptake and of alanine excretion displayed an inverse relationship. Ammonium uptake, alanine and branch chain amino acid release were all dependent on the concentration of l-malate displaying similar K0.5 values of 0.5 mM demonstrating concerted regulation. The hyperbolic kinetics of ammonium uptake and amino acid excretion suggests transport via a membrane carrier and also suggested that transport was rate limiting. Glutamate uptake displayed exponential kinetics implying transport via a channel. The chemical nature of the compounds released were dependent upon bacteroid density, partial pressure of oxygen and concentration of l-malate demonstrating an integrated metabolism.
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Dissertations / Theses on the topic "Malate transport"

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Charbonnier, Teddy. "Transport du pyruvate et régulations du métabolisme central par le malate chez Bacillus subtilis." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLS076/document.

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Chez Bacillus subtilis comme pour toutes les bactéries, le métabolisme central du carbone est essentiel pour la croissance de la cellule. Elle utilise le glucose (source de carbone glycolytique) et le malate (source de carbone gluconéogenique) comme sources de carbone préférentielles. Ces deux sources de carbone sont capables d'induire la répression catabolique au travers de la protéine régulatrice CcpA et ainsi d'établir une hiérarchie dans l'utilisation des sources alternatives de carbone. Au centre du métabolisme du carbone se trouve le pyruvate que B. subtilis est capable d'utiliser comme seule source de carbone, mais son transporteur reste inconnu.Des analyses transcriptomiques ont montré que seul l'opéron ysbAB était spécifiquement induit en présence de pyruvate, et nous avons montré que sa délétion entraînait une perte de croissance presque totale sur pyruvate. En utilisant des protéines étiquetées, nous avons mis en évidence qu'YsbA et YsbB formaient un complexe se localisant à la membrane. Nous avons ensuite montré que ce complexe est le transporteur principal du pyruvate et fonctionne comme un transporteur par diffusion facilitée. A l'aide d'une fusion rapportrice, nous avons démontré que l'opéron lytST situé en amont d'ysbAB, et codant pour un système à deux composants, était responsable de l'induction d'ysbAB. Nous avons également montré qu'en plus d'une répression par CcpA en présence de glucose ou de malate, une régulation dépendante de l'activité enzyme malique de MaeA s'exerce sur ysbAB. Cette régulation est due à l'accumulation de pyruvate dans la cellule qui perturbe l'activation d'ysbAB par LytST.Nous avons aussi montré qu'une régulation indépendante de CcpA s'exerce sur dctP, le gène codant pour le transporteur du succinate et du fumarate en présence de malate, suggérant un mécanisme similaire à celui observé pour ysbAB. Enfin, nous avons montré que le flux métabolique traversant MaeA était également impliqué dans la régulation par CcpA de l'entrée des sources glycolytique par le malate
In Bacillus subtilis like for all the bacteria, the central carbon metabolism is essential for growth. It uses glucose (a glycolytic carbon source) and malate (a gluconeogenic carbon source) as preferential carbon sources. These two carbon sources are able to induce carbon catabolite repression through the transcription factor CcpA and thus establishing a hierarchy in the use of alternative carbon sources. The pyruvate is in the middle of the carbon metabolism, and can be used by B. subtilis as sole carbon source; however its transporter remains unknown.Transcriptome analyses revealed that the only operon specifically expressed in cells grown on pyruvate is ysbAB, and we showed that its deletion led to a strong growth defect on pyruvate. Using tagged proteins, we highlighted that YsbA and YsbB formed a complex localized at the membrane. We next showed that this complex is the major pyruvate transporter, and operates as a facilitated transporter. Using a reporter fusion, we showed that the operon lytST located upstream of ysbAB, and coding for a two-component system, is responsible for the induction of ysbAB. We also showed that besides the CcpA-mediated repression by both glucose and malate, an additional regulation mechanism through the malic enzyme activity of MaeA is acting on ysbAB. This regulation is due to the accumulation of pyruvate in the cell which hinders the LytST-mediated induction of ysbAB.We also showed that a CcpA-independent repression is exerted on dctP, the gene coding for the succinate and fumarate transporter, in the presence of malate, suggesting a regulation mechanism similar to the one observed for ysbAB. Finally, we showed that the metabolic flux going through MaeA is also involved in the CcpA-dependent repression of the genes coding for glycolytic transporter in presence of malate
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Saayman, Maryna. "Characterisation of the malate transporter and malic enzyme from Candida utilis." Thesis, Stellenbosch : University of Stellenbosch, 2011. http://hdl.handle.net/10019.1/16520.

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Dissertation (PhD)--University of Stellenbosch, 2005.
ENGLISH ABSTRACT: Yeast species differ remarkably in their ability to degrade extracellular dicarboxylic acids and to utilise them as their only source of carbon. The fission yeast Schizosaccharomyces pombe effectively degrades L-malate, but only in the presence of an assimilable carbon source. In contrast, the yeast Saccharomyces cerevisiae is unable to effectively degrade L-malate, which is ascribed to the slow uptake of L-malate by diffusion. In contrast, the yeast Candida utilis can utilise L-malate as the only source of carbon and energy, but this is subject to substrate induction and catabolite repression. Very little research has been done on a molecular level in C. utilis and only a few of its genes have been studied. In this study, we have shown that the yeast C. utilis effectively degraded extracellular L-malate and fumarate, but in the presence of glucose or other assimilable carbon sources, the transport and degradation of these dicarboxylic acids was repressed. The transport of both dicarboxylic acids was shown to be strongly inducible by either L-malate or fumarate and kinetic studies suggest that the same transporter protein transports the two dicarboxylic acids. In contrast, S. pombe effectively degraded extracellular L-malate, but not fumarate, only in the presence of glucose or other assimilable carbon sources. The S. pombe malate transporter was unable to transport fumarate, although fumarate inhibited the uptake of L-malate. In order to clone the C. utilis dicarboxylic acid transporter, a cDNA library from C. utilis was constructed using a number of strategies to ensure representativeness and high transformation frequencies. The cDNA library was transformed in a S. cerevisiae strain carrying a plasmid containing the S. pombe malic enzyme gene (mae2) to allow screening for a malate-degrading S. cerevisiae clone. However, no positive clones that would indicate the successful cloning of the C. utilis malate transporter were obtained. The C. utilis malic enzyme gene, CuME, was subsequently isolated from the cDNA library based on conserved sequence homologies with the genes of S. cerevisiae and S. pombe, and characterised on a molecular and biochemical level. Sequence analysis revealed an open reading frame of 1926 bp, encoding a 641 amino acid polypeptide with a predicted molecular weight of 70.2 kDa. The optimum temperature for the C. utilis malic enzyme was 52°C and the enzyme was stable at 50°C for 2 hours. The inferred amino acid sequence showed significant homology with the malic enzymes of S. pombe and S. cerevisiae. Expression of the CuME gene is subject to glucose repression and substrate induction, as was observed for the dicarboxylic acid transporter from C. utilis. The CuME gene was successfully coexpressed with the S. pombe malate permease gene (mae1), resulting in a recombinant strain of S. cerevisiae able to effectively degrade L-malate.
AFRIKAANSE OPSOMMING: Daar is ’n merkwaardige verskil in die vermoë van verskillende gisspesies om ektrasellulêre dikarboksielsure af te breek en dit as enigste bron van koolstof te benut. Die splitsingsgis Schizosaccharomyces pombe kan L-malaat effektief afbreek, maar slegs in die teenwoordigheid van ’n ander benutbare koolstofbron. In teenstelling hiermee is dit vir die gis Saccharomyces cerevisiae onmoontlik om L-malaat effektief af te breek en te benut, wat hoofsaaklik toegeskryf kan word aan die stadige opname van L-malaat deur middel van diffusie. Die gis Candida utilis kan egter L-malaat as die enigste bron van koolstof en energie benut, maar dit is onderhewig aan substraat-induksie en kataboliet onderdrukking. Baie min navorsing op molekulêre vlak is tot hede in C. utilis uitgevoer en slegs ’n paar gene in hierdie gis is al bestudeer. In hierdie studie het ons aangetoon dat die gis C. utilis L-malaat en fumaraat effektief afbreek, maar dat glukose of ander benutbare koolstofbronne die opname en afbraak van hierdie dikarboksielsure onderdruk. Die opname van beide dikarboksielsure is sterk induseerbaar deur L-malaat óf fumaraat, terwyl kinetiese studies toon dat beide dikarboksielsure deur dieselfde transporter-proteïen vervoer word. In teenstelling hiermee kan S. pombe ekstrasellulêre L-malaat, maar nie fumaraat nie, in die teenwoordigheid van glukose of ’n ander benutbare koolstofbron effektief afbreek. Die S. pombe L-malaat transporter was nie in staat om fumaraat te vervoer nie, alhoewel fumaraat die opname van L-malaat onderdruk het. Ten einde die dikarboksielsuur transporter van C. utilis te kloneer, is verskeie strategieë gevolg ten einde ’n cDNA-biblioteek van C. utilis te konstrueer wat verteenwoordiging en hoë transformasie-frekwensies kan verseker. Die cDNA-biblioteek is getransformeer in ’n S. cerevisiae ras wat die S. pombe malaatensiem geen (mae2) bevat om die sifting van ’n S. cerevisiae kloon wat malaat effektief kan afbreek, moontlik te maak. Geen positiewe klone wat dui op die klonering van die C. utilis malaat transporter kon egter gevind word nie. Die C. utilis malaatensiem geen, CuME, is vervolgens van uit die cDNA biblioteek geïsoleer deur van gekonserveerde DNA-homologie met S. cerevisiae en S. pombe gebruik te maak, en op molekulêre en biochemiese vlak gekarakteriseer. DNA-volgordebepaling het ’n oopleesraam van 1926 bp onthul, wat kodeer vir ’n 641 aminosuur polipeptied met ’n verwagte molekulêre gewig van 70.2 kDa. Die optimale temperatuur van die C. utilis malaatensiem was 52°C en die ensiem was vir 2 ure stabiel by 50°C. Die afgeleide aminosuurvolgorde het beduidende homologie met die malaatensieme van S. pombe en S. cerevisiae getoon. Die CuME geen is suksesvol saam met die S. pombe malaat permease geen (mae1) uitgedruk om ’n rekombinante S. cerevisiae ras te genereer wat in staat is om L-malaat effektief af te breek.
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Li, Youzhong, and Youzhong Li@health gov au. "Respiration and nitrogen fixation by bacteroids from soybean root nodules : substrate transport and metabolism in relation to intracellular conditions." The Australian National University. Faculty of Science, 2003. http://thesis.anu.edu.au./public/adt-ANU20040630.114138.

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Bacteroids of B. japonicum from nodules of soybean roots were isolated using differential centrifugation (the standard bench method) and density gradient centrifugation methods (either sucrose- or Percoll-) under anaerobic conditions in which N2 fixation was preserved. The relationships between N2 fixation and respiration, O2 supply, O2 demand, substrate (mainly malate) transport and metabolism in bacteroids were investigated using the flow chamber system. In related experiments, the primary products of N2 fixation which leave the bacteroids were investigated using a 15N-labelling technique in a closed shaken system and other biochemical methods.¶ In the flow chamber experiments, the rates at which O2 was supplied to bacteroids in the chamber were varied by (a) changing the flow rate of reaction medium through the chamber; (b) by changing the [O2 free] in the inflowing reaction medium by using either 3-5% (v/v) or 100% air in the gas mixture above the stirred reaction medium in two reservoir flasks; (c) by successively withdrawing bacteroids from the chamber, thus increasing the supply of O2 per bacteroid to those remaining in the chamber. The results showed that the rate of O2 supply regulates respiratory demand for O2 by bacteroids rather than the O2 concentration present in the reaction system. Respiration is always coupled to N2 fixation. ¶ Uptake of malate by bacteroids withdrawn from the flow chamber was measured under microaerobic conditions. Malate uptake by these N2-fixing bacteroids was lower than that by bacteroids isolated under aerobic conditions, which eliminate N2 fixation of bacteroids, but is closely correlated with bacteroid respiration rates. When respiration was increased by an increase in O2 supply, malate uptake by bacteroids was also increased. This suggested that transport of malate through the bacteroid membrane is also regulated by O2 supply, but indirectly. Higher uptake by bacteroids under aerobic conditions was observed because respiration was enhanced by the high availability of O2, but the fast uptake of malate by bacteroids driven by the abnormal respiration rates may not reflect the reality of malate demand in vivo by bacteroids when N2 fixation by bacteroids is fully coupled. ¶ The results of 15N labelling experiments and other biochemical assays once again demonstrated that ammonia is the principal significant 15N labelled product of N2 fixation accumulated during 30 min in shaken assays with 0.008-0.01 atm O2. Alanine although sometimes found in low concentrations in the flow chamber reactions, was not labelled with 15N in shaken closed system experiments. No evidence could be obtained from the other biochemical assays, either. Therefore, it is concluded that these and earlier results were not due to contamination with host cytosolic enzymes as suggested by Waters et al. (Proc. Natl. Aca. Sci. 95, 1998, pp 12038-12042). ¶ Malate transported into bacteroids is oxidized in a modified TCA cycle present in bacteroids. The results of flow chamber experiments with a sucA mutant (lacking a-ketoglutarate dehydrogenase) showed that respiratory demand for O2 by the mutant bacteroids is regulated by O2 supply in the same way as the wild-type. Despite differences in other symbiotic properties, rates of nitrogen fixation by the mutant bacteroids, based on the bacteroid dry weight, appeared to be the same as in the wild-type. Also N2 fixation was closely coupled with respiration in the same manner in both mutant bacteroids and wild type bacteroids. These results and other supporting data, strongly support the conclusion that there is an alternative pathway of the TCA cycle in bacteroids, which enables the missing step in the mutant to be by-passed with sufficient activity to support metabolism of transported malate.
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Vogl, Malte [Verfasser], and Tobias [Akademischer Betreuer] Brandes. "Collective and critical effects in bosonic transport / Malte Vogl. Betreuer: Tobias Brandes." Berlin : Universitätsbibliothek der Technischen Universität Berlin, 2013. http://d-nb.info/103199369X/34.

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MUNOZ, RIVERO MANUEL. "Evaluation medicale des transports primaires heliportes." Aix-Marseille 2, 1989. http://www.theses.fr/1989AIX20230.

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GUILLEMIN, OLIVIER. "Transport des nouveau-nes de tres faible poids : a propos de 122 cas d'enfants de poids de naissance inferieur ou egal a 1 500 grammes transportes par le samu de lyon." Lyon 1, 1993. http://www.theses.fr/1993LYO1M351.

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BOSSARD, SYLVAIN. "Bilan des transports intra-hospitaliers des patients adultes effectues par le samu 44 du 01. 01. 1988 au 31. 12. 1988." Nantes, 1990. http://www.theses.fr/1990NANT146M.

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DUPEYRE, CHRISTOPHE. "Les helistations et helisurfaces des hopitaux et cliniques de midi-pyrenees." Toulouse 3, 1994. http://www.theses.fr/1994TOU31022.

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BERREGHIS, SORAYA. "Le transport heliporte des malades cardiaques au samu 72 : bilan, incidents, perspectives." Nantes, 1993. http://www.theses.fr/1993NANT048M.

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Morel, Marina. "Régulation du transport des mitochondries dans les neurones et expression des moteurs moléculaires dans le cortex humain: implication pour l'étude des anomalies du transport axoplasmique dans la maladie d'Alzheimer." Doctoral thesis, Universite Libre de Bruxelles, 2011. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209885.

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La maladie d’Alzheimer est la maladie neurodégénérative la plus fréquente dans le monde industrialisé. Sur le plan neuropathologique, cette maladie est caractérisée par deux types de lésions :les plaques séniles et les dégénérescences neurofibrillaires.

Des observations morphologiques précédentes ont permis de mettre en évidence des anomalies du transport axoplasmique dans les neurones chez les patients atteints de la maladie d’Alzheimer. Les mécanismes menant à cette perturbation du transport axoplasmique ne sont pas encore bien établis. La glycogen synthase kinase-3β (GSK-3β) et la cyclin-dependent kinase 5 (Cdk5) associée à son activateur pathologique p25, sont deux kinases clés dont la dérégulation intervient dans la pathogenèse de la maladie d’Alzheimer (MA). Nous avons émis l'hypothèse que ces kinases pourraient jouer un rôle dans la perturbation du transport axoplasmique dans cette maladie.

Dans la première partie de notre travail, nous nous sommes intéressés à l’effet de la GSK-3β et de Cdk5/p25 sur la croissance des neurites (un processus dépendant du transport axoplasmique) dans un modèle cellulaire, les PC12 différenciées prétraitées au NGF.

La surexpression de GSK-3β et de p25 provoque une importante réduction de la croissance neuritique dans ces cellules. Par western blot, nous avons montré que cette réduction est associée à des modifications post-traductionnelles des protéines impliquées dans la régulation du cytosquelette. Ces modifications sont la phosphorylation de la protéine tau et des neurofilaments et l’acétylation de la tubuline α.

Cette étude indique donc que la GSK-3β et la protéine p25 contrôlent négativement la croissance neuritique.

Dans la deuxième partie de notre travail, afin d’étudier la relation entre ces kinases et le transport axoplasmique, nous avons analysé dans des neurones en culture l’effet d’une augmentation d’activité de la GSK-3β et de Cdk5/p25 sur le transport des mitochondries.

Pour étudier le déplacement des mitochondries, les neurones en cultures ont été doublement transfectées avec deux plasmides :un marqueur mitochondrial combiné avec la GSK-3β ou p25. Après transfection, le mouvement des mitochondries a été enregistré grâce à la technique du time-lapse.

L’étude de la fréquence de trois comportements (mouvement antérograde, mouvement rétrograde et état stationnaire) nous a indiqué que les mitochondries sont normalement en position immobile pendant 70 % de leur temps. La surexpression de GSK-3β ou de p25 augmente la fréquence de cet état stationnaire et diminue de manière plus importante les mouvements antérogrades que rétrogrades sans affecter la vitesse des mitochondries. L’observation au microscope électronique a permis de démontrer la persistance du réseau de microtubules dans les cellules surexprimant GSK-3β ou p25.

Le transport des mitochondries est un processus actif faisant intervenir les moteurs moléculaires (kinésine et dynéine) dont le rôle est le transport d’organelles qui repose sur un réseau intact de microtubules.

Cette étude suggère donc que la GSK-3β et p25 contrôlent négativement le transport des mitochondries en agissant au niveau des moteurs moléculaires (kinésine et dynéine) plutôt qu’en détruisant le réseau de microtubules.

Dans la troisième partie de notre travail, nous nous sommes intéressés à l’expression et à la localisation dans le cortex frontal humain et dans le cortex cérébelleux de deux protéines appartenant aux moteurs moléculaires responsables des transports axoplasmiques antérograde et rétrograde :la chaîne légère de la kinésine (KLC1) et la chaîne intermédiaire de la dynéine (DIC).

Nous avons observé une diminution du niveau d’expression de la KLC1 et de la DIC dans le cortex frontal (une zone atteinte dans la MA) mais pas dans le cortex cérébelleux chez les patients atteints de la maladie d’Alzheimer par rapport à des sujets contrôles. Une diminution du niveau d’expression de la tubuline-β3 et de la synaptophysine -deux marqueurs neuronaux- a aussi été observée dans le cortex frontal mais pas dans le cortex cérébelleux. Nous avons aussi démontré une hausse de l’état de phosphorylation de la KLC1 dans un modèle cellulaire surexprimant la GSK-3β. Dans le cortex frontal dans la MA, nous avons observé une augmentation de la forme active de la GSK-3β, et une hausse de la phosphorylation de la KLC1. Cette phosphorylation accrue de la KLC1 diminue son activité de transport des organelles.

Ces anomalies de l’expression et de la phosphorylation des moteurs moléculaires pourraient jouer un rôle dans les perturbations des transports axoplasmiques dans la MA.


Doctorat en Sciences biomédicales et pharmaceutiques
info:eu-repo/semantics/nonPublished

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Books on the topic "Malate transport"

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D, Patterson Christina, ed. Critical care patient transport: Principles and practice. 5th ed. [Suffolk, Va.]: Critical Care Concepts, 2008.

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Langlet, Michèle. Ergonomie et soins infirmiers: La santé des soignants. Paris: Lamarre, 1990.

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Advanced emergency care and transportation of the sick and injured. 2nd ed. Boston: Jones & Bartlett Learning, 2012.

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Emergency medical responder: A skills approach. 2nd ed. Toronto: Pearson Prentice Hall, 2006.

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Emergency medical responder: A skills approach. 3rd ed. Toronto: Pearson Canada, 2009.

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Association, National Fire Protection, and International Association of Fire Chiefs, eds. Vehicle extrication level I & II: Principles and practice. Boston: Jones & Bartlett Learning, 2012.

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Minor, Mary Alice D. Patient care skills: Documentation, vital signs, bandaging, aseptic techniques, positioning, range of motion, wheelchairs, and transfer. 2nd ed. Norwalk, Conn: Appleton & Lange, 1990.

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Duesterhaus, Minor Scott, ed. Patient care skills. 6th ed. Upper Saddle River, N.J: Pearson Education, 2010.

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Duesterhaus, Minor Scott, ed. Patient care skills. 3rd ed. Norwalk, Conn: Appleton & Lange, 1995.

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Minor, Mary Alice D. Patient care skills. 4th ed. Stamford, Conn: Appleton & Lange, 1999.

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Book chapters on the topic "Malate transport"

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Martinoia, E., and D. Rentsch. "Uptake of Malate and Citrate into Plant Vacuoles." In Transport and Receptor Proteins of Plant Membranes, 101–9. Boston, MA: Springer US, 1992. http://dx.doi.org/10.1007/978-1-4615-3442-6_9.

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Lu, Ming, Suhanti Banerjee, Gerald M. Saidel, and Xin Yu. "Regulation of Cytosolic and Mitochondrial Oxidation via Malate-Aspartate Shuttle: An Observation Using Dynamic 13C NMR Spectroscopy." In Oxygen Transport to Tissue XXXII, 185–92. Boston, MA: Springer US, 2011. http://dx.doi.org/10.1007/978-1-4419-7756-4_25.

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Ellis, L. C., and B. R. Nemetallah. "Sperm maturation and transport." In Male Fertility and Its Regulation, 397–409. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-4894-5_27.

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Rousseau, G. G., J. I. Quivy, C. F. Rolin Jacquemyns, D. A. N. Sirett, M. T. de Reviers, M. C. Viguier-Martinez, S. Delpech, and G. Colas. "Non-steroidal inhibitors of androgen transport and metabolism." In Male Fertility and Its Regulation, 331–42. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-4894-5_22.

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Wright, Tessa. "Gender Segregation in the Construction and Transport Sectors." In Gender and Sexuality in Male-Dominated Occupations, 61–84. London: Palgrave Macmillan UK, 2016. http://dx.doi.org/10.1057/978-1-137-50136-3_3.

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Esteves, Sandro, and Deborah Spaine. "Sperm Transport and Maturation." In Medical and Surgical Management of Male Infertility, 14. Jaypee Brothers Medical Publishers (P) Ltd., 2014. http://dx.doi.org/10.5005/jp/books/11840_3.

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Sylvester, Steven R., and Michael D. Griswold. "Molecular Biology of Iron Transport in the Testis." In Molecular Biology of the Male Reproductive System, 311–26. Elsevier, 1993. http://dx.doi.org/10.1016/b978-0-08-091764-1.50013-1.

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Twidale, C. R. "Granitic Terrains." In The Physical Geography of Southeast Asia. Oxford University Press, 2005. http://dx.doi.org/10.1093/oso/9780199248025.003.0019.

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Granite underlies substantial areas of Southeast Asia. It forms the core of many of the major uplands. Yet exposures are scarce. High rainfall, consistently high temperatures, and the naturally abundant vegetation have together caused the granite to be deeply weathered. Most of the land surface is underlain by a more or less thick mantle of weathered rock or regolith. Only where the regolith has been removed by natural agencies, for example on some hill crests and steep midslopes, in river channels, and in coastal areas, is the bedrock naturally exposed, though road cuttings, quarries, and other artificial excavations provide excellent sections. Anthropogenically induced and accelerated soil erosion have also revealed bedrock morphology in places. The granitic terrains consist essentially of high ridges rising abruptly from the valley floors or adjacent plains. In detail, slopes, river channels, and rocky coasts strewn with granite blocks and boulders are characteristic of the region, and the nature of granite weathering has also influenced the character of the sediment load transported to rivers and coasts. Though the granites of Southeast Asia are well documented geologically and as sources of tin and other minerals, there are few modern accounts of their geomorphological aspects. Early travellers like Logan (1848) made astute observations relevant to the development of granitic forms, and the officers of the geological surveys of Malaya and, later, of Malaysia have, taking their lead from the first director onwards, noted salient features of the granitic terrains they mapped. These observations and interpretations, taken together with the few specifically geomorphological studies of particular features, and analyses of granitic landforms in other countries, permit the granitic terrains of Southeast Asia to be placed in context. Granitic rocks are widely distributed in Southeast Asia, particularly in the mainland states (Hutchison 1989). Those of the Malay Peninsula were emplaced at various depths: shallow epizonal, deep catazonal, but mostly mesozonal emplacement at 5–11 km depth. In plan, granites are widely distributed (Gobbett and Tjia 1973; Chinese Geosciences Research Institute 1975; UNESCO 1980). In the Malay Peninsula, granites occupy the cores of major regional anticlines, and many plutons are exposed in the breached crests of such structures.
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Beinart, William, and Lotte Hughes. "Rubber and the Environment in Malaysia." In Environment and Empire. Oxford University Press, 2007. http://dx.doi.org/10.1093/oso/9780199260317.003.0019.

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The rise of the motor car created two very different commodity frontiers in the British Empire, one producing oil and the other rubber. The demand for rubber followed an often-repeated pattern in that it was shaped by scientific invention, technological change, and new patterns of consumption in the industrialized world. It was related directly to the development of new fossil fuels. Coal transformed shipping and overland transport by rail. Oil (Chapter 15) opened new realms for mobility. The invention in 1867 of the internal combustion engine by a German, Nikolaus Otto, and in 1885 of automobiles powered by gasoline-driven engines revolutionized transport, culture, and the South-East Asian environment. During the late nineteenth century, wild natural rubber booms swept through the tropical world, from Brazil to the Congo, leaving in their wake hardship and scandal. In Malaysia, there was a very different outcome—the development of plantations on a new capitalist agrarian frontier. Rubber became one of the single most important commodities produced in the Empire, and was enormously valuable to Britain not only for its own motor industry but also to sell to the United States. Whereas demand for some earlier imperial commodities was largely British, there was also significant consumption of rubber and oil in other parts of the Empire, especially the settler dominions. In the early decades of the twentieth century, rubber plantations, in parallel with expanding sugar production in Queensland, Natal, Trinidad, and Fiji, extended and intensified Britain’s engagement with the tropical zones of the world. Indentured workers replaced slaves as the major plantation workforce. South India was the major labour source for Malaysia, where the ports and tin-mining centres already had substantial Chinese communities. British colonialism in Malaysia left as its legacy a multi-ethnic society. By the 1930s about 55 per cent were indigenous Malays and Orang Asli, 35 per cent of Chinese origin, and close to 10 per cent Indian. Although capital was increasingly mobile by the late nineteenth century, extraction and production of the three major commodities of the twentieth century Empire proved to be highly location specific. Gold and oil were trapped in particular geological formations.
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Simpson, Juliet. "Imaging the Uncanny Memory." In W.G. Sebald’s Artistic Legacies. Nieuwe Prinsengracht 89 1018 VR Amsterdam Nederland: Amsterdam University Press, 2023. http://dx.doi.org/10.5117/9789463729758_ch02.

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“A man lives not only his personal life, as an individual, but also, consciously or unconsciously, the life of his epoch and his contemporaries.” Thomas Mann’s 1924 words on war, memory, and art also distils his emblematic encounter with Matthias Grünewald’s Isenheim Altarpiece.1 Transported from Colmar to Munich in October 1917, exhibited from 1918–19 at the Munich Alte Pinakothek, as this article explores, it became the focus of an extraordinary moment of German national crisis and expiation widely imaged, projected, reported, and seen by countless visitors including Mann. My concerns are two-fold. First, to explore responses in word and image stimulated by the Altarpiece’s display and extensive photographic imaging in the contexts of a significant cultural shift toward engagement with the potency of medieval art, and its mediation of practices of “uncanny” memory. Entwined with an acute sense of present trauma, this activates what Hans Belting calls the borderlines between memory and image; devotion and distance2—pivoting in 1918–19 on the Altarpiece’s power as an afterlife, restaged to make present what appears absent: a medieval turbulence as a contemporary imaging and consciousness of pain. Second is to consider how this process becomes amplified in the aftermath of the war, in particular, via its writing as an unheimlich past in Thomas Mann’s The Magic Mountain, echoed in Marc Bloch’s invocation of a material memory of power, malady and sacred touch in his Les Rois thaumaturges, both 1924. Thus the conclusions suggest the recurrent, yet overlooked artistic figure of pre-modern memory within this constellation as pivotal to an imaginary of the unimaginable of what is seen and unseen.
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Conference papers on the topic "Malate transport"

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GRINDLAY, ALEJANDRO L., and SERGIO MARTÍNEZ-HORNOS. "CITY–PORT RELATIONSHIPS IN MALAGA, SPAIN: EFFECTS OF THE NEW PORT PROPOSALS ON URBAN TRAFFIC." In URBAN TRANSPORT 2017. Southampton UK: WIT Press, 2017. http://dx.doi.org/10.2495/ut170051.

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Fu, Yu, Zhe Jia, Lu Yu, Xingsi Zhong, and Richard Brooks. "A covert data transport protocol." In 2016 11th International Conference on Malicious and Unwanted Software (MALWARE). IEEE, 2016. http://dx.doi.org/10.1109/malware.2016.7888734.

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de Paula Diniz, Daniel, Ana Katarina Pessoa de Oliveira, and Monica Carvalho. "PEGADA DE CARBONO ASSOCIADA AO TRANSPORTE DE MALTE EM CERVEJARIAS NO NORDESTE BRASILEIRO." In XVII Encontro Nacional de Estudantes de Engenharia Ambiental e V Fórum Latino-Americano de Engenharia e Sustentabilidade. Recife, Brasil: Even3, 2019. http://dx.doi.org/10.29327/15304.17-155555.

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Sastre González, Julián, Maria Cuello León, Cinta Romero Adame, and Noemí Vaquero Redondo. "¿Qué es un DOT? Análisis de casos." In CIT2016. Congreso de Ingeniería del Transporte. Valencia: Universitat Politècnica València, 2016. http://dx.doi.org/10.4995/cit2016.2016.3182.

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Son claros los efectos negativos del patrón de crecimiento urbano disperso y del enfoque de la planificación del transporte desde la oferta, en vez de hacerlo desde la demanda. Las medidas que buscan un incremento de la oferta de infraestructura viaria (mediante construcción de nuevas o ampliación de las existentes) con el fin de satisfacer la demanda creciente de tráfico motorizado, orientadas a la mejora del desplazamiento en vehículo privado, no sólo son insuficientes para solucionar el problema de la movilidad urbana, sino que, favorecen el crecimiento de los viajes en coche y con ello generan una mayor congestión. La idea del desarrollo sostenible, que propone crecer económicamente con equidad, sin dañar los ecosistemas y superando la pobreza, es una referencia obligada en las diversas actividades y disciplinas. El urbanismo no es la excepción, sobre todo si se considera que la ciudad transforma de manera intensa el medio ambiente. Por lo tanto, el urbanismo tiene una estrecha relación con la propuesta de sostenibilidad. Aparece una nueva tendencia conjuga urbanismo y movilidad, es el denominado DOT: “Desarrollo Urbano Orientado al Transporte Público”. Esta tendencia se basa en el desarrollo urbano en áreas adyacentes a estaciones de transporte masivo con el objetivo de fomentar un mayor uso del sistema de transporte público y a la vez potenciar la sostenibilidad financiera de dicho sistema mediante la concentración de la demanda de usuarios del transporte público a partir de la re /densificación urbana en zonas estratégicas. Los desarrollos DOT se diseñan según ocho principios ligados al urbanismo sostenible: Caminar, Pedalear, Conectar, Transportar, Mezclar, Densificar, Compactar y Cambiar. Se analizan tres experiencias internacionales de DOT estudiando la aplicación de cada uno de los principios DOT a través de un análisis comparativo de buenas y malas prácticas.DOI: http://dx.doi.org/10.4995/CIT2016.2016.3182
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González Peréz, Luis Miguel, Johan Wideberg, and Borja GONZALEZ PEREZ-SOMARRIBA. "Bicycling-related accidents and factors contributing to injury." In CIT2016. Congreso de Ingeniería del Transporte. Valencia: Universitat Politècnica València, 2016. http://dx.doi.org/10.4995/cit2016.2016.3718.

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Objective: This study was conducted to find the epidemiological characteristics of bicycling-related maxillofacial fractures in a defined population, and identify factors contributing to injury. Methodology: A prospective study was carried out involving patients presenting with maxillofacial fractures sustained in bicycling-related accidents. Results: Between 908 of all cycling accidents attending for medical treatment, 122 patients (13% of all cycling accidents) were admitted with facial fractures between 2007 and 2014. Male and female ratio was 2.6:1, and the mean age was 29.4 years (standard deviation: 12.8, range: 12-79 years). Causes of injury included collisions (63%) and accidental falls (37%). The fracture patterns seen were mandibular (49%), zygomatic (32%), orbital (13%), nasal (7%), maxillary (2%), and frontal (2%). Condylar fractures were the most common of the mandibular fractures (63%). The most frequently observed concomitant lesions were orthopedic injuries. Conclusions: Bicycling-related maxillofacial injuries are common and therefore important to identify in order to design a sustainable transport system and for units that provide assistance to traffic accident victims. Missed diagnosis or delayed treatment can lead to facial deformities and functional problems. Wearing protective helmets and the improvement of the helmets design is one aspect that would be of interest for the prevention of injuries. Keywords: Cycling; bicycle-related trauma; maxillofacial fractures; risk factors; helmets.DOI: http://dx.doi.org/10.4995/CIT2016.2016.3718
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Berrones Sanz, Luis David, and Victoria Alejandra Muro Báez. "Accidentes viales de los motociclistas en México: subgrupos y factores de riesgo." In CIT2016. Congreso de Ingeniería del Transporte. Valencia: Universitat Politècnica València, 2016. http://dx.doi.org/10.4995/cit2016.2016.2172.

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Motorcycling as a means of transportation has grown rapidly in Mexico. In the last decade, the number of motorcycles increased 338.05%, which means that motorbikes represent 5.97% of the total number of vehicles in the country. During this period, however, the annual average shows that 3.16% of motorcyclists had an accident, which is proof that motorcycle riders are vulnerable users with high risks in terms of road safety. The objective of this research is to identify the subgroups of motorcyclers with higher accident risks and to identify risk factors. The sample for the quantitative study included all motorbike riders who died or were injured as a result of a reported road accident, between 2000 and 2014. The sample was obtained using the database at the National Institute for Statistics and Geography and the General Direction of Health Information. Descriptive variables were determined for all the categories and were matched to the death cause to find statistical correlation. These systems of information registrered, during 2014 in Mexico, more than 41,881 accidents and 826 deaths caused by motorcycle accident. The highest number of accidents involved men (P<0.001) between 20 and 30 years old, where 20.55% had head injuries and only 16.59% of bikers were wearing a helmet at the time of the accident. This combination – age 20-30, male gender and not wearing a helmet – seems to be a powerful risk factor. However, accidents are largely underreported due to the fact that the format used to receive attention after suffering a violent attack or injury (SIS-SS-127-P) does not include the motorcycle as an agent of injury. For this reason, it is of the utmost importance to create a reliable statistical system and promote a good road safety culture together with protective factors and safety equipment.DOI: http://dx.doi.org/10.4995/CIT2016.2016.2172
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Shanley, Kevin T., Goodarz Ahmadi, Philip K. Hopke, and Yung-Sung Cheng. "Fibrous and Spherical Particle Transport and Deposition in the Human Nasal Airway: A Computational Fluid Dynamics Model." In ASME 2009 Fluids Engineering Division Summer Meeting. ASMEDC, 2009. http://dx.doi.org/10.1115/fedsm2009-78204.

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As the interface between the human respiratory system and the environment, the nose plays many vital roles. Not the least of which is filter. Resulting from numerous natural and anthropogenic processes, particulate matter becomes airborne. Should particulate matter reach the lower portions of the respiratory tract, a host of maladies may occur. In an attempt to further understand the physics behind particulate matter transitioning from the environment into humans a computational model has been developed to predict the efficiency with which human noses can remove particles before they reach the lungs. To this end computational fluid dynamics and Lagrangian particle tracking simulations have been run to gather information on the deposition behavior of both fibrous and spherical particles. MRI data was collected from the left and right passages of a 181.6 cm, 120.2 kg, human male. The two passages were constructed into separate computational volumes consisting of approximately 950,000 unstructured tetrahedral cells each. A steady laminar flow model was used to simulate the inhalation portion of a human breathing cycle. Volumetric flow rates were varied to represent the full range of human nasal breathing. General agreement was shared quantitatively and qualitatively with previously published in vitro studies on other nasal models. Lagrangian particle tracking was performed for varying sizes of fibrous and spherical particles. Deposition efficiency was shown to increase with fiber aspect ratio, particle size, and flow rate. Anatomy was also identified as effecting deposition.
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Brooks, Joseph Bruno Bidin. "De novo variant in the MAPK8IP3 gene in the differential diagnosis of global development delay. Case report." In XIII Congresso Paulista de Neurologia. Zeppelini Editorial e Comunicação, 2021. http://dx.doi.org/10.5327/1516-3180.181.

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Context: The global development delay has a high prevalence and heterogeneity in the world population. With the advancement of technology and detection of pathogenic variants detected by sequencing the exome, genes related to global developmental delay could be identified and collaborate for further clinical clarification. Among the studied genes, the MAPK8IP3 gene, became an attractive candidate due to its performance in neuronal axonal transport in vertebrates and invertebrates. This case report was approved by the Ethics Committee of Universidade Metropolitana de Santos. Case Report: The present case refers to a 6-year-old male patient presenting with a clinical picture of global developmental delay without bodily dysmorphia. Cerebellar ataxia, muscle hypotonia and intellectual impairment are important clinical impairments. Skull MRI and complementary exams were normal. The genetic study showed a new and heterozygous pathogenic variant in the MAPK8IP3 gene. Conclusions: Symptomatic treatment with multiprofessional rehabilitation was instituted with partial improvement of symptoms.
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Shaghaghian, Sana, Arash Naseri, Omid Abouali, and Goodarz Ahmadi. "Numerical Simulation of the Virtual Maxillary Sinus Surgery Effects on the Heat Transfer in Human Nasal Airway." In ASME/JSME/KSME 2015 Joint Fluids Engineering Conference. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/ajkfluids2015-26371.

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Uncinectomy and middle meatal antrostomy (MMA) in the maxillary sinus surgery mainly affects the airflow pattern in this sinus. The aim of the present work was to analyze the effect of this surgery on the heating and humidifying function of the nose. A series of CT scan images of a healthy male volunteer was used and a computational model for the human nasal airway including nasal cavity and maxillary sinuses was developed. Then, uncinectomy and MMA was performed virtually on the CT images on a single nasal passage and associated maxillary sinus. The continuity, momentum, energy and moisture transport equations were solved numerically. In particular, a thermal model for evaluating the temperature and moisture distribution on the mucus surface covering the walls of the nasal airway was developed. A steady breathing flow rate related to the rest conditions was investigated, where different relative humidity levels for the ambient air were considered. The airflow pattern, temperature and moisture concentration contours for pre- and post-surgery cases were evaluated and their differences were discussed.
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Yuy, N. D., N. N. Malyutina, and E. A. Leskovets. "THE MAIN ASPECTS OF THE LOCOMOTIVE WORKERS’ INDUSTRIAL MEDICAL REHABILITATION AFTER COVID-19." In The 16th «OCCUPATION and HEALTH» Russian National Congress with International Participation (OHRNC-2021). FSBSI “IRIOH”, 2021. http://dx.doi.org/10.31089/978-5-6042929-2-1-2021-1-601-606.

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Annotation. This article highlights the issues of workers’ industrial medical rehabilitation, one of the leading professions of railway transport who had already had COVID 19. There is a main aspect is the data of the studies carried out for the period 2020-2021 there on the basis of the Center for Restorative Medicine and Rehabilitation of the Private Healthcare Institution KB "Russian Railways-Medicine" Yekaterinburg and the Clinical Hospital of the ChUZ of Perm together with the Department of Faculty Therapy No. 2 of Occupational Pathology and Clinical Laboratory Diagnostics of PSMU im. Wagner. Proceeding from the fact that the institutions of Russian Railways-Medicine are the main link in maintaining the health of the transport system of JSC Russian Railways employees. Methods: On the basis of the rehabilitation department in Yekaterinburg, express questionnaires about quality of life were developed and implemented for JSC Russian Railways employees after this disease. The questionnaires included a subjective assessment of the physiological, psychological, and emotional state of Russian Railways employees (able-bodied) who had recovered in the near future and those who had not more than six months after the illness. The total number of people who have recovered since the beginning of the pandemic is about 4,000. The questionnaires received on feedback from 761 people were processed. In the structure of the interviewed by gender: male - 47%, female - 53%, respondents who have had mild and moderate forms of a new coronavirus infection (COVID-19). In the research and analysis of the material obtained, the criteria for selection for rehabilitation were identified, taking into account age and gender status, as well as the need for rehabilitation measures. The obtained data were compared with the research data of the Department of Faculty Therapy No. 2, Occupational Pathology and Clinical Laboratory Diagnostics of the Vagner State Medical Academy, Perm. Results: The study results are used in the development of rehabilitation programs and are also aimed at reducing industrial risks and hazards arising in the railway transport when performing functions by employees of locomotive crews who returned to production activities in the early period after this infection. Since, in combination with postcovid symptoms, the influence of production factors, the “specificity of the human factor”, can lead to serious consequences, both in health and in the performance of their labor duties on the infrastructure of railway transport, which is an increased danger zone. We have found that rehabilitation measures must be carried out for all employees of Russian Railways who have been ill, regardless of gender, age and degree of lung damage. In our opinion, an important marker for the understanding of functional disorders in postcoid symptomatology is the neutropicity of the virus, its penetration and damage to the limbic system, those important departments responsible for the regulation of general changes in brain activation that affect the integrity of functioning.
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Reports on the topic "Malate transport"

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Beck, Aaron. RiverOceanPlastic: Land-ocean transfer of plastic debris in the North Atlantic, Cruise No. AL534/2, 05 March – 26 March 2020, Malaga (Spain) – Kiel (Germany). GEOMAR Helmholtz Centre for Ocean Research Kiel, 2020. http://dx.doi.org/10.3289/cr_al534-2.

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Cruise AL534/2 is part of a multi-disciplinary research initiative as part of the JPI Oceans project HOTMIC and sought to investigate the origin, transport and fate of plastic debris from estuaries to the oceanic garbage patches. The main focus of the cruise was on the horizontal transfer of plastic debris from major European rivers into shelf regions and on the processes that mediate this transport. Stations were originally chosen to target the outflows of major European rivers along the western Europe coast between Malaga (Spain) and Kiel (Germany), although some modifications were made in response to inclement weather. In total, 16 stations were sampled along the cruise track. The sampling scheme was similar for most stations, and included: 1) a CTD cast to collect water column salinity and temperature profiles, and discrete samples between surface and seafloor, 2) sediment sampling with Van Veen grab and mini-multi corer (mini-MUC), 3) suspended particle and plankton sampling using a towed Bongo net and vertical WP3 net, and 4) surface neusten sampling using a catamaran trawl. At a subset of stations with deep water, suspended particles were collected using in situ pumps deployed on a cable. During transit between stations, surface water samples were collected from the ship’s underway seawater supply, and during calm weather, floating litter was counted by visual survey teams. The samples and data collected on cruise AL534/2 will be used to determine the: (1) abundance of plastic debris in surface waters, as well as the composition of polymer types, originating in major European estuaries and transported through coastal waters, (2) abundance and composition of microplastics (MP) in the water column at different depths from the sea surface to the seafloor including the sediment, (3) abundance and composition of plastic debris in pelagic and benthic organisms (invertebrates), (4) abundance and identity of biofoulers (bacteria, protozoans and metazoans) on the surface of plastic debris from different water depths, (5) identification of chemical compounds (“additives”) in the plastic debris and in water samples.
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2

Lenz, Mark. RV POSEIDON Fahrtbericht / Cruise Report POS536/Leg 1. GEOMAR, October 2020. http://dx.doi.org/10.3289/geomar_rep_ns_56_2020.

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DIPLANOAGAP: Distribution of Plastics in the North Atlantic Garbage Patch Ponta Delgada (Portugal) – Malaga (Spain) 17.08. – 12.09.2019 The expedition POS 536 is part of a multi-disciplinary research initiative of GEOMAR investigating the origin, transport and fate of plastic debris from estuaries to the oceanic garbage patches. The main focus will be on the vertical transfer of plastic debris from the surface and near-surface waters to the deep sea and on the processes that mediate this transport. The obtained data will help to develop quantitative models that provide information about the level of plastic pollution in the different compartments of the open ocean (surface, water column, seafloor). Furthermore, the effects of plastic debris on marine organisms in the open ocean will be assessed. The cruise will provide data about the: (1) abundance of plastic debris with a minimum size of 100 μm as well as the composition of polymer types in the water column at different depths from the sea surface to the seafloor including the sediment, (2) abundance and composition of plastic debris in organic aggregates (“marine snow”), (3) in pelagic and benthic organisms (invertebrates and fish) and in fecal pellets, (4) abundance and the identity of biofoulers (bacteria, protozoans and metazoans) on the surface of plastic debris from different water depths, (5) identification of chemical compounds (“additives”) in the plastic debris and in water samples.
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3

Izhar, Shamay, Maureen Hanson, and Nurit Firon. Expression of the Mitochondrial Locus Associated with Cytoplasmic Male Sterility in Petunia. United States Department of Agriculture, February 1996. http://dx.doi.org/10.32747/1996.7604933.bard.

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The main goal of the proposed research was to continue the mutual investigations into the molecular basis of CMS and male fertility restoration [MRF], with the ultimate goal of understanding these phenomena in higher plants. The experiments focused on: (1) dissecting apart the complex CMS - specific mitochondrial S-Pcf locus, in order to distinguish its essential parts which cause sterility from other parts and study its molecular evolution. (2) Studying the expression of the various regions of the S-Pcf locus in fertile and sterile lines and comparing the structure and ultrastructure of sterile and fertile tissues. (3) Determine whether alteration in respiration is genetically associated with CMS. Our mutual investigations further substantiated the association between the S-Pcf locus and CMS by the findings that the fertile phenotype of a population of unstable petunia somatic hybrids which contain the S-Pcf locus, is due to the presence of multiple muclear fertility restoration genes in this group of progenies. The information obtained by our studies indicate that homologous recombination played a major role in the molecular evolution of the S-Pcf locus and the CMS trait and in the generation of mitochondrial mutations in general. Our data suggest that the CMS cytoplasm evolved by introduction of a urs-s containing sublimon into the main mitochondrial genome via homologous recombination. We have also found that the first mutation detected so far in S-Pcf is a consequence of a homologous recombination mechanism involving part of the cox2 coding sequence. In all the cases studied by us, at the molecular level, we found that fusion of two different cells caused mitochondrial DNA recombination followed by sorting out of a specific mtDNA population or sequences. This sequence of events suggested as a mechanism for the generation of novel mitochondrial genomes and the creation of new traits. The present research also provides data concerning the expression of the recombined and complex CMS-specific S-Pcf locus as compared with the expression of additional mitochondrial proteins as well as comparative histological and ultrastructural studies of CMS and fertile Petunia. Evidence is provided for differential localization of mitochondrially encoded proteins in situ at the tissue level. The similar localization patterns of Pcf and atpA may indicate that Pcf product could interfere with the functioning of the mitochondrial ATPase in a tissue undergoing meiosis and microsporogenesis. Studies of respiration in CMS and fertile Petunia lines indicate that they differe in the partitioning of electron transport through the cytochrome oxidase and alternative oxidase pathways. The data indicate that the electron flux through the two oxidase pathways differs between mitochondria from fertile and sterile Petunia lines at certain redox states of the ubiquinone pool. In summary, extensive data concerning the CMS-specific S-Pcf locus of Petunia at the DNA and protein levels as well as information concerning different biochemical activity in CMS as compared to male fertile lines have been accumulated during the three years of this project. In addition, the involvement of the homologous recombination mechanism in the evolution of mt encoded traits is emphasized.
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4

McKinnon, Mark, Sean DeCrane, and Steve Kerber. Four Firefighters Injured in Lithium-Ion Battery Energy Storage System Explosion -- Arizona. UL Firefighter Safety Research Institute, July 2020. http://dx.doi.org/10.54206/102376/tehs4612.

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On April 19, 2019, one male career Fire Captain, one male career Fire Engineer, and two male career Firefighters received serious injuries as a result of cascading thermal runaway within a 2.16 MWh lithium-ion battery energy storage system (ESS) that led to a deflagration event. The smoke detector in the ESS signaled an alarm condition at approximately 16:55 hours and discharged a total flooding clean agent suppressant (Novec 1230). The injured firefighters were members of a hazardous materials (HAZMAT) team that arrived on the scene at approximately 18:28 hours. The HAZMAT team noted low-lying white clouds of a gas/vapor mixture issuing from the structure and nearby components and drifting through the desert. The team defined a hot zone and made several entries into the hot zone to conduct 360-degree size-ups around the ESS using multi-gas meters, colorimetric tubes, and thermal imaging cameras (TICs). The team detected dangerously elevated levels of hydrogen cyanide (HCN) and carbon monoxide (CO) during each entry. The team continued to monitor the ESS and noted the white gas/vapor mixture stopped flowing out of the container at approximately 19:50 hours. The HAZMAT leadership developed an incident action plan with input from a group of senior fire officers and information about the ESS provided by representatives from the companies that owned, designed, and maintained the ESS. The HAZMAT team made a final entry into the hot zone and found that HCN and CO concentrations in the vicinity of the ESS were below an acceptable threshold. In following with the incident action plan, the team opened the door to the ESS at approximately 20:01 hours. A deflagration event was observed by the firefighters outside the hot zone at approximately 20:04 hours. All HAZMAT team members received serious injuries in the deflagration and were quickly transported to nearby hospitals. Note: The lithium-ion battery ESS involved in this incident was commissioned prior to release of a first draft of the current consensus standard on ESS installations, NFPA 855 [1]; the design of the ESS complied with the pertinent codes and standards active at the time of its commissioning.
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5

Firon, Nurit, Prem Chourey, Etan Pressman, Allen Hartwell, and Kenneth J. Boote. Molecular Identification and Characterization of Heat-Stress-Responsive Microgametogenesis Genes in Tomato and Sorghum - A Feasibility Study. United States Department of Agriculture, October 2007. http://dx.doi.org/10.32747/2007.7591741.bard.

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Exposure to higher than optimal temperatures - heat-stress (HS) - is becoming increasingly common to all crop plants worldwide. Heat stress coinciding with microgametogenesis, especially during the post-meiotic phase that is marked by starch biosynthesis, is often associated with starch-deficient pollen and male sterility and ultimately, greatly reduced crop yields. The molecular basis for the high sensitivity of developing pollen grains, on one hand, and factors involved in pollen heat-tolerance, on the other, is poorly understood. The long-term goal of this project is to provide a better understanding of the genes that control pollen quality under heat-stress conditions. The specific objectives of this project were: (1) Determination of the threshold heat stress temperature(s) that affects tomato and sorghum pollen quality whether: a) Chronic mild heat stress conditions (CMHS), or b) Acute heat stress (AHS). (2) Isolation of heat-responsive, microgametogenesis-specific sequences. During our one-year feasibility project, we have accomplished the proposed objectives as follows: Objectrive 1: We have determined the threshold HS conditions in tomato and sorghum. This was essential for achieving the 2nd objective, since our accumulated experience (both Israeli and US labs) indicate that when temperature is raised too high above "threshold HS levels" it may cause massive death of the developing pollen grains. Above-threshold conditions have additional major disadvantages including the "noise" caused by induced expression of genes involved in cell death and masking of the differences between heatsensitive and heat-tolerant pollen grains. Two different types of HS conditions were determined: a) Season-long CMHS conditions: 32/26°C day/night temperatures confirmed in tomato and 36/26°C day maximum/night minimum temperatures in sorghum. b) Short-term AHS: In tomato, 2 hour exposure to 42-45°C (at 7 to 3 days before anthesis) followed by transfer to 28/22±2oC day/night temperatures until flower opening and pollen maturation, caused 50% reduced germinating pollen in the heat-sensitive 3017 cv.. In sorghum, 36/26°C day/night temperatures 10 to 5 days prior to panicle emergence, occurring at 35 days after sowing (DAS) in cv. DeKalb28E, produced starch-deficient and sterile pollen. Objective 2: We have established protocols for the high throughput transcriptomic approach, cDNA-AFLP, for identifying and isolating genes exhibiting differential expression in developing microspores exposed to either ambient or HS conditions and created a databank of HS-responsivemicrogametogenesis-expressed genes. A subset of differentially displayed Transcript-Derived Fragments (TDFs) that were cloned and sequenced (35 & 23 TDFs in tomato and sorghum, respectively) show close sequence similarities with metabolic genes, genes involved in regulation of carbohydrate metabolism, genes implicated in thermotolerance (heat shock proteins), genes involved in long chain fatty acids elongation, genes involved in proteolysis, in oxidation-reduction, vesicle-mediated transport, cell division and transcription factors. T-DNA-tagged Arabidopsis mutants for part of these genes were obtained to be used for their functional analysis. These studies are planned for a continuation project. Following functional analyses of these genes under HS – a valuable resource of genes, engaged in the HS-response of developing pollen grains, that could be modulated for the improvement of pollen quality under HS in both dicots and monocots and/or used to look for natural variability of such genes for selecting heat-tolerant germplasm - is expected.
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6

Fowler, Joanna, and Michael Furey. An open-label, randomized positron emission tomography (PET)study in healthy male volunteers consisiting of Part A and Part B.Part A: Clinical validation of norepinephrine transporter (NET) PET ligand, (S,S)-[11C]O-methylreboxetine ([11C]MRB) using different doses of oral atomoxetine as NET reuptake inhibitor.Part B: Evaluation of NET occupancy, as measured by [11C]MRB, with multiple dosing regimens of orally administered GSK372475. Office of Scientific and Technical Information (OSTI), August 2007. http://dx.doi.org/10.2172/973576.

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