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Cribb, Elizabeth J. "Golden2 gene function in maize leaf development." Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.326139.
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Earl, Hugh J. "Estimating leaf photosynthesis in maize using chlorophyll fluorometry." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ31883.pdf.
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Clayton, Helen. "Carbohydrate oxidation in maize bundle sheath." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335719.
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So, Yoon-Sup. "Corn leaf aphid and polysora rust resistance in tropical maize." Thesis, University of Hawaii at Manoa, 2003. http://hdl.handle.net/10125/7012.
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This research includes two separate studies both of which incorporated generation mean analyses to interpret genetically the resistance to com leaf aphid and polysora rust in tropical maize. The first study focused on resistance to corn leaf aphid in tropical sweet corn inbred Hi38-71. An inoculation technique using hair-pin clip cages and infestation quantification method using digital image analysis were developed for this study. During the study, a heavy natural infestation of com leaf aphids occurred in a seed production nursery. Yield loss by ranged from 38.9% to 98.8%, with an average loss of 71.7%. The clip-cage method was effective in distinguishing resistant and susceptible plants under field conditions. Resistance to com leaf aphid from Hi38-71 appeared to be monogenic and recessive. Aphid reproduction and population growth were measured on four different genotypes of varying aphid tolerance. Aphids on Hi38-71 had poorest performance over all aspects of growth and reproduction examined. Difference in number of progenies produced and days to 50% mortality appeared to account for most of the difference observed in the genetic study. The second study estimated genetic parameters for polysora rust resistance in Hi38-71. Hi38-71 exhibited moderately high resistance to polysora rust as well as resistance to com leaf aphid. Generation mean analysis showed that epistatic interactions of [aa] and [dd] along with simple dominance and additive gene effects were involved in controlling resistance in Hi38-71 to polysora rust. It is concluded that polysora resistance breeding cannot be based on selection of a single parent but a hybrid-breeding or reciprocal recurrent selection approach appears justified. The tropical sweet corn inbred, Hi38-71 is a sib line of Hi38 which was bred from a bt-1 conversion of AA8sh2. AA8sh2 was studied for its resistance to corn leaf aphid in 1970's in Hawaii and was converted to common rust resistance, Rd1-D which broke down due to evolved racial variation of the pathogen. Hi38-71 is thus of particular value in sweet corn breeding for tropical regions. This is due not only to its resistance to corn leaf aphid and polysora, but to its high sweet corn qualities and generally good combining abilities.xiii, 86 leaves
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Lehmensiek, Anke. "Genetic mapping of gray leaf spot resistance genes in maize." Thesis, Stellenbosch : Stellenbosch University, 2000. http://hdl.handle.net/10019.1/51776.
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Thesis (PhD)--Stellenbosch University, 2000.ENGLISH ABSTRACT: Gray leaf spot (GLS) of maize, caused by the fungus Cercospora zeae-maydis, can reduce grain yields by up to 60% and it is now recognized as one of the most significant yield-limiting diseases of maize in many parts of the world. The most sustainable and long-term management strategy for GLS will rely heavily on the development of high-yielding, locally adapted GLS resistant hybrids. Molecular markers could be useful to plant breeders to indirectly select for genes affecting GLS resistance and to identify resistance genes without inoculation and at an early stage of plant development. Only two studies in the USA have examined quantitative trait loci (QTL) association with GLS resistance. The aim of this study was to map GLS resistance genes in a resistant Seed Co LTD, Zimbabwean inbred line. Molecular markers linked to the GLS resistance QTL were identified by using the amplified fragment length polymorphism (AFLP) technique together with bulked segregant analysis. Eleven polymorphic AFLP fragments were identified and converted to sequence-specific PCR (polymerase chain reaction) markers. Eight of the 11 converted AFLP markers were added to the maize marker database of the University of Stellenbosch. Five of the 8 converted AFLP markers were polymorphic between the resistant and the susceptible parent. They were amplified on the DNA of 230 plants of a segregating F2 population and linkage analysis was performed with MAPMAKER/EXP. Two linkage groups consisting of two markers each, with a linkage distance of 10.4 cM (LOD 22.83) and 8.2 cM (LOD 55.41) between the two markers, were identified. QTL mapping with MAPMAKER/QTL confirmed the presence of QTL in both linkage groups. Two publicly available recombinant inbred families (Burr et a/., 1988) were used to localize the converted AFLP markers on the genetic map of maize. The QTL, which were identified with the AFLP markers, were mapped to chromosomes 1 and 5. Another AFLP marker was mapped to chromosome 2 and a further to chromosome 3. To obtain more precise localizations of the QTL on chromosomes 1 and 5, sequence-tagged site markers and microsatellite markers were used. The markers were amplified on the DNA of the 230 plants of the F2 population and linkage analysis was performed with MAPMAKER/EXP. The order of the markers was in agreement with the UMC map of the Maize Genome Database. Interval mapping using MAPMAKERlQTL and composite interval mapping using QTL Cartographer were performed. The QTL on chromosome 1 had a LOD score of 21 and was localized in bin 1.05/06. A variance of 37% was explained by the QTL. Two peaks were visible for the QTL on chromosome 5, one was localized in bin 5.03/04 and the other in bin 5.05/06. Both peaks had a LOD score of 5 and 11% of the variance was explained by the QTL. To test the consistency of the detected QTL, the markers flanking each QTL were amplified on selected plants of two F2 populations planted in consecutive years and regression analysis was performed. Both the QTL on chromosome 1 and the QTL on chromosome 5 were detected in these populations. Furthermore, the presence of a QTL on chromosome 3 was confirmed with these populations. A variance of 8 -10% was explained by the QTL on chromosome 3. In this study, a major GLS resistance QTL was thus mapped on chromosomes 1 and two minor GLS resistance QTL were mapped on chromosomes 3 and 5 using a resistant Seed Co LTD, Zimbabwean inbred line. Markers were identified which could be used in a marker-assisted selection program to select for the GLS resistance QTL.
AFRIKAANSE OPSOMMING: Grys blaarvlek (GBV) van mielies, veroorsaak deur die swam Cercospora zeaemaydis, kan graanopbrengs met tot 60% verlaag en word beskou as een van die vernaamste opbrengs-beperkende siektes wêreldwyd. Die toepaslikste langtermyn stragtegie vir GBV beheer sal wees om plaaslike mieliebasters met hoë opbrengs en GBV weerstand te ontwikkel. Molekulêre merkers kan nuttig deur plantetelers gebruik word om weerstandsgene te selekteer. Seleksie is moontlik in die afwesigheid van inokolum en op 'n vroeë stadium van plant ontwikkeling. Slegs twee vorige studies (in die VSA) het kwantitatiewe-kenmerk-Iokusse (KKL), vir GBVweerstand ondersoek. Die doel van hierdie studie was om die GBV weerstandsgene in 'n weerstandbiedende ingeteelde lyn (Seed Co BPK, Zimbabwe) te karteer. Molekulêre merkers gekoppel aan die GBV weerstands KKL is geïdentifiseer deur gebruik te maak van die geamplifiseerde-fragmentlengte-polimorfisme- (AFLP-) tegniek en gebulkte-segregaat-analise. Elf polimorfiese merkers is geïdentifiseer en omgeskakel na volgorde-spesifieke PKR (polimerase kettingreaksie) merkers. Agt van die elf omgeskakelde AFLP-merkers is by die mieliemerker databasis van die Universiteit van Stellenbosch gevoeg. Vyf van die 8 omgeskakelde AFLP-merkers was polimorfies tussen die bestande en vatbare ouers. Hulle is geamplifiseer op die DNA van 230 plante van 'n segregerende F2-populasie en is gebruik in 'n koppelingstudie met MAPMAKER/EXP. Twee koppelingsgroepe, elk bestaande uit twee merkers, met onderskeidelik koppelingsafstande van 10.4 eM (LOD 22.83) en 8.2 eM (LOD 55.41) tussen die merkers, is geïdentifiseer. KKL-kartering het getoon dat KKL in albei koppelingsgroepe aanwesig is. Twee kommersieël beskikbare, rekombinant-ingeteelde families (Burr et aI., 1988) is gebruik om die omgeskakelde AFLP-merkers op die mielie genetiese kaart te plaas. Die KKL wat met die AFLP-merkers geïdentifiseer is, is gekarteer op chromosome 1 en 5. 'n Verdere AFLP-merker is op chromosoom 2 gekarteer en 'n ander op chromosoom 3. Ten einde die KKL op chromosome 1 en 5 meer akkuraat te karteer, is volgordege- etikeerde en mikrosatelliet merkers gebruik. Die merkers is geamplifiseer op die DNA van die 230 plante van die F2-populasie en koppelings-analises is uitgevoer. Die volgorde van die merkers was dieselfde as die van die UMC-kaart in die Mielie Genoom Databasis. Interval kartering met MAPMAKER/QTL en komposiet interval kartering met QTL Cartographer is uitgevoer. Die KKL op chromosoom 1 het 'n LOD-telling van 21 gehad en is in bin 1.05/06 geplaas. Die KKL was verantwoordelik vir 37% van die variansie. Twee pieke was onderskeibaar vir die KKL op chromosoom 5, een in bin 5.03/04 geleë en die ander in bin 5.05/06. Elke piek het 'n LOD-telling van 5 gehad en die twee KKL was verantwoordelik vir 11% van die variansie. Om die herhaalbaarheid van die effek van die KKL te toets is die merkers naaste aan elke KKL geamplifiseer op geselekteerde plante van twee F2-populasies wat in opeenvolgende jare geplant is. Regressie analise is op die data gedoen. Beide die KKL op chromosoom 1 en die KKL op chromosoom 5 kon in hierdie populasies geïdentifiseer word. Verder kon die aanwesigheid van 'n verdere KKL op chromosoom 3 in hierdie populasies bevestig word. Laasgenoemde KKL was verantwoordelik vir 8-10% van die totale variansie. In hierdie studie is daar dus 'n hoof GBV-weerstands KKL gekarteer op chromosoom 1 en twee kleiner GBV-weerstands KKL gekarteer op chromosome 3 en 5. Merkers is geïdentifiseer wat moontlik in merker-gebaseerdetelingsprogramme gebruik kan word om plante te selekteer wat die GBVweerstands KKL het.
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Gallagher, Kimberly L. "Analysis of asymmetric cell divisions in the maize leaf epidermis /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2000. http://wwwlib.umi.com/cr/ucsd/fullcit?p3007134.
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Roth, Ronelle. "Phenotypic characterization of maize bundle sheath defective mutants." Thesis, University of Oxford, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339349.
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Du, Min. "A greenhouse screening method for resistance to gray leaf spot in maize." Thesis, Virginia Tech, 1993. http://hdl.handle.net/10919/42953.
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Mwangi, Symon Munanda. "Status of Northen Leaf Blight, Phaeosphaeria maydis Leaf Spot, Southern Leaf Blight, Rust, Maize Streak Virus and Physiologic Specialization of Exserohilum turcicum in Kenya." Diss., Virginia Tech, 1998. http://hdl.handle.net/10919/26093.
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Exserohilum turcicum, Phaeosphaeria maydis, Bipolaris maydis, Puccinia polysora, Puccinia sorghi and maize streak virus were the most serious maize pathogens found in Kenya during a 1995/96 survey of 65 farms representing all the agro-ecological zones. Exserohilum turcicum - induced blight was observed in all maize producing areas with disease incidence reaching over 45%. Lesions were sometimes over 400 mm long by 25 mm in width. Southern leaf blight caused by Bipolaris maydis was most severe in the Kenyan maize granary located in the highlands west of the Rift Valley where it occurred alone or with Phaeosphaeria maydis leaf spot. Incidences of over 75% were recorded for southern leaf blight and Phaeosphaeria maydis leaf spot in western Kenya and over 85% for Phaeosphaeria maydis leaf spot in Thika, Nyeri, Muranga and Kirinyaga Districts of Central Province. Common rust caused by Puccinia sorghi was equally severe in the areas of Molo, Njoro and Bahati in Nakuru District with severity levels of over 65%. Lowland rust caused by Pucinia polysora was important in the coastal region with over 75% disease severity in Kwale District. Traces of lowland rust were observed in Kirinyaga District. Maize streak virus was severe in the Lake Victoria region of western Kenya, central areas of Kenya and in the Coast. This disease was severe where Napier grass (Pennisetum purpureum) with streak like symptoms was near the infected maize plots. Individual cultures of Exserohilum turcicum were not appreciably different in appearance but varied in growth rates at 22oC. A total of nineteen isolates of Exserohilum turcicum were classified into races using the Ht gene bearing maize differential inbred lines. Three isolates were race 0, three were race 1, one was race 2, one was race 3, two isolates were race 12, and eight did not fit in the known classification. These were named and grouped as k1, k2 and k3. The k3 race was virulent to HtN gene only. Four Kenyan and two American lines were inoculated with eight isolates of Exserohilum turcicum. All the Kenyan lines were susceptible to the isolates used. Tables of means comparisons and figures for disease occurrence and severity are provided. This is the first time disease prevalence and severity has been documented for maize in Kenya.Ph. D.
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Dhau, Inos. "Detection, identification, and mapping of maize streak virus and grey leaf spot diseases of maize using different remote sensing techniques." Thesis, University of Limpopo, 2019. http://hdl.handle.net/10386/2866.
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Thesis (PhD. (Geography)) --University of Limpopo, 2019Of late climate change and consequently, the spread of crop diseases has been identified as one of the major threat to crop production and food security in subSaharan Africa. This research, therefore, aims to evaluate the role of in situ hyperspectral and new generation multispectral data in detecting maize crop viral and fungal diseases, that is maize streak virus and grey leaf spot respectively. To accomplish this objective; a comparison of two variable selection techniques (Random Forest’s Forward Variable, (FVS) and Guided Regularized Random Forest: (GRRF) was done in selecting the optimal variables that can be used in detecting maize streak virus disease using in-situ resampled hyperspectral data. The findings indicated that the GRRF model produced high classification accuracy (91.67%) whereas the FVS had a slightly lower accuracy (87.60%) based on Hymap when compared to the AISA. The results have shown that the GRRF algorithm has the potential to select compact feature sub sets, and the accuracy performance is better than that of RF’s variable selection method. Secondly, the utility of remote sensing techniques in detecting the geminivirus infected maize was evaluated in this study based on experiments in Ofcolaco, Tzaneen in South Africa. Specifically, the potential of hyperspectral data in detecting different levels of maize infected by maize streak virus (MSV) was tested based on Guided Regularized Random Forest (GRRF). The findings illustrate the strength of hyperspectral data in detecting different levels of MSV infections. Specifically, the GRRF model was able to identify the optimal bands for detecting different levels of maize streak disease in maize. These bands were allocated at 552 nm, 603 nm, 683 nm, 881 nm, and 2338 nm. This study underscores the potential of using remotely sensed data in the accurate detection of maize crop diseases such as MSV and its severity which is critical in crop monitoring to foster food security, especially in the resource-limited subSaharan Africa. The study then investigated the possibility to upscale the previous findings to space borne sensor. RapidEye data and derived vegetation indices were tested in detecting and mapping the maize streak virus. The results revealed that the use of RapidEye spectral bands in detection and mapping of maize streak virus disease yielded good classification results with an overall accuracy of 82.75%. The inclusion of RapidEye derived vegetation indices improved the classification accuracies by 3.4%. Due to the cost involved in acquiring commercial images, like xviii RapidEye, a freely available Landsat-8 data can offer a new data source that is useful for maize diseases estimation, in environments which have limited resources. This study investigated the use of Landsat 8 and vegetation indices in estimating and predicting maize infected with maize streak virus. Landsat 8 data produced an overall accuracy of 50.32%. The inclusion of vegetation indices computed from Landsat 8 sensor improved the classification accuracies by 1.29%. Overally, the findings of this study provide the necessary insight and motivation to the remote sensing community, particularly in resource-constrained regions, to shift towards embracing various indices obtained from the readily-available and affordable multispectral Landsat-8 OLI sensor. The results of the study show that the mediumresolution multispectral Landsat 8-OLI data set can be used to detect and map maize streak virus disease. This study demonstrates the invaluable potential and strength of applying the readily-available medium-resolution, Landsat-8 OLI data set, with a large swath width (185 km) in precisely detecting and mapping maize streak virus disease. The study then examined the influence of climatic, environmental and remotely sensed variables on the spread of MSV disease on the Ofcolaco maize farms in Tzaneen, South Africa. Environmental and climatic variables were integrated together with Landsat 8 derived vegetation indices to predict the probability of MSV occurrence within the Ofcolaco maize farms in Limpopo, South Africa. Correlation analysis was used to relate vegetation indices, environmental and climatic variables to incidences of maize streak virus disease. The variables used to predict the distribution of MSV were elevation, rainfall, slope, temperature, and vegetation indices. It was found that MSV disease infestation is more likely to occur on low-lying altitudes and areas with high Normalised Difference Vegetation Index (NDVI) located at an altitude ranging of 350 and 450 m.a.s.l. The suitable areas are characterized by temperatures ranging from 24°C to 25°C. The results indicate the potential of integrating Landsat 8 derived vegetation indices, environmental and climatic variables to improve the prediction of areas that are likely to be affected by MSV disease outbreaks in maize fields in semi-arid environments. After realizing the potential of remote sensing in detecting and predicting the occurrence of maize streak virus disease, the study further examined its potential in mapping the most complex disease; Grey Leaf Spot (GLS) in maize fields using WorldView-2, Quickbird, RapidEye, and Sentinel-2 resampled from hyperspectral data. To accomplish this objective, field spectra were acquired from healthy, moderate and xix severely infected maize leaves during the 2013 and 2014 growing seasons. The spectra were then resampled to four sensor spectral resolutions – namely WorldView-2, Quickbird, RapidEye, and Sentinel-2. In each case, the Random Forest algorithm was used to classify the 2013 resampled spectra to represent the three identified disease severity categories. Classification accuracy was evaluated using an independent test dataset obtained during the 2014 growing season. Results showed that Sentinel-2 achieved the highest overall accuracy (84%) and kappa value (0.76), while the WorldView-2, produced slightly lower accuracies. The 608 nm and 705nm were selected as the most valuable bands in detecting the GLS for Worldview 2, and Sentinel-2. Overall, the results imply that opportunities exist for developing operational remote sensing systems for detection of maize disease. Adoption of such remote sensing techniques is particularly valuable for minimizing crop damage, improving yield and ensuring food security.
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Donahue, Patrick J. "Inheritance of reactions to gray leaf spot and maize dwarf mosaic virus in maize and their associations with physiological traits." Diss., Virginia Polytechnic Institute and State University, 1989. http://hdl.handle.net/10919/54518.
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Gray leaf spot, caused by Cercospora zeae-maydis, can be a yield-limiting factor in maize where continuous minimum tillage practices are followed. Commercial corn hybrids were evaluated for response to gray leaf spot for seven years at two Virginia locations (Shenandoah and Wythe Counties) and one year at a third location in Virginia (Montgomery County). Yield losses, when comparing resistant to susceptible classes, were approximately 2,000 kg ha⁻¹ at Wythe County in 1982, 750 kg ha⁻¹ at Shenandoah County in 1984, and 2,150 kg ha⁻¹ at Montgomery County in 1988. The inheritance of reaction to gray leaf spot was studied using a 14 inbred diallel in Montgomery and Wythe Counties, Virginia in 1987 and 1988 planted in randomized complete block designs. Resistance was found to be highly heritable and controlled by additive gene action. Inbreds producing high yielding, resistant, and agronomically superior hybrids were identified (B68, NC250, Pa875, Va14, Va17, and Va85); and several hybrids between these lines had high levels of resistance, high yield, and good general agronomic characters (B68 x KB1250, KB1250 x Pa875, and NC250 x Pa875). Currently available inbreds could be used to produce hybrids with higher levels of resistance than hybrids currently available to growers, and these could serve as a basis for gray leaf spot breeding programs. Lesion size measurements were not correlated with disease scores. Late-season photosynthesis rates were associated positively with resistance. The hybrids of some inbreds were found to produce high levels of pigment (believed to be anthocyanins) around the gray leaf spot lesions. These did not limit the size of the individual lesion later in the season. Some pigment(s)-producing genotypes were found to be resistant when the pigment character was expressed. This type of resistance must prevent or inhibit infection of the leaf but not later colonization, once established. Maize dwarf mosaic virus (MDMV) also limits maize production in some areas where johnsongrass (Sorghum halepense L.) is a problem. Resistance to MDMV was found to be mainly additive and highly heritable. However, a strong specific combining ability component was found, indicating that the background of the material receiving resistance genes may have a strong effect on the expression of resistance. Inbreds capable of producing high-yielding, resistant, and agronomically acceptable hybrids are available (B68, NC250, A632, Pa875, Va17, and Va85); and several hybrids between these lines have high levels of resistance, high yield, and good general agronomic characters (B68 x KB1250, KB1250 x Pa875, and NC250 x Pa875).Ph. D.
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Christie, Nanette. "Transcriptional regulation underlying the quantitative genetic response of maize to grey leaf spot disease." Thesis, University of Pretoria, 2014. http://hdl.handle.net/2263/79215.
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Cercospora zeina causes grey leaf spot (GLS), a yield-limiting disease on maize. The main objective of this study was to exploit maize gene expression data to dissect the quantitative disease response to C. zeina infection. The project addresses the hypothesis that there is an underlying DNA polymorphism that gives rise to a change in gene expression, which in turn affects GLS disease severity. Genomic and functional annotation of the reporters on an Agilent 44K maize microarray was carried out. This microarray was used for global gene expression profiling of earleaf samples collected from 100 recombinant inbred sub-tropical maize lines exposed in the field to C. zeina. Gene expression profiles together with GLS severity scores were used in a weighted gene co-expression network analysis to identify co-expression modules associated with disease severity. Quantitative trait locus (QTL) mapping for GLS severity was combined with expression QTL (eQTL) analyses to investigate the molecular basis of the quantitative response to GLS. An Eqtl data analysis pipeline was developed in Galaxy. The overlap of phenotypic QTLs with cis- and trans-eQTLs revealed putative causal candidate genes and potential mechanisms responsible for the QTLs, respectively. Regulatory network models were constructed for trans-eQTL hotspots coinciding with phenotypic QTLs. A genetic basis for coordinated expression responses to GLS disease was identified. For the susceptible response, the results lead to the hypothesis that a calmodulin-related protein with a cis-eQTL acts as a global regulator of various pathogenesis-related proteins that are activated too late after infection started. For the resistant response, it is hypothesised that a serine threonineprotein kinase with a cis-eQTL acts as a post-translational global regulator regulating phosphatases and kinases involved in activation of defense gene expression. The outcomes of this study were: i) the development of a systems genetics strategy and ii) several hypotheses of maize transcriptional responses to C. zeina which need to be validated with further studies. These results extend the current knowledge of GLS resistance and could aid in the improvement of maize varieties.Thesis (PhD)--University of Pretoria, 2014.
Biochemistry
PhD
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Hughes, Thomas. "The genetic regulation of Kranz anatomy in maize." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:86184e64-c7bb-43e9-9320-0ebbb2793ea8.
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The C4 photosynthetic pathway acts to concentrate CO2 around the enzyme Ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco), ensuring that it catalyses a carboxylation rather than oxygenation reaction, which in turn suppresses photorespiration. In nearly all cases C4 photosynthesis is underpinned by characteristic Kranz anatomy, with concentric wreaths of bundle sheath (BS) and mesophyll (M) cells surrounding closely spaced veins. The increased yields associated with the C4 pathway have lead to the suggestion that C3 crops such as rice should be engineered to undertake C4 photosynthesis, however, this goal is currently held back by a lack of understanding about how the development of Kranz anatomy is regulated. Recently, a number of candidate Kranz regulators have been identified in an RNA-seq study that compared leaf development in maize foliar (Kranz) and husk (non-Kranz) leaves. However, this study did not consider the impact of a recent whole genome duplication in the maize lineage on the gene expression patterns analysed. Therefore, in this thesis maize homeolog gene-pair divergence during early leaf development was assessed. This revealed that expression divergence of homeolog gene-pairs is a significant evolutionary phenomenon. Functional validation of a subset of Kranz candidates revealed that a Zmscr1-1; Zmscr1h-1 double mutant exhibited defects in Kranz patterning, including increased formation of extra BS cells and veins with no separating M cells. Furthermore, Zmnkd1; Zmnkd2 double mutants exhibited a subtle increase in extra BS cell formation. Taken together, this indicates that both ZmSCR1/ZmSCR1h and ZmNKD1/ZmNKD2 function redundantly during Kranz development. No evidence was obtained that two additional genes, ZmSHR2 and ZmRVN1, play a role in Kranz development, and expression of candidate Kranz regulators in rice did not alter leaf anatomy. Together, this work has confirmed roles for a number of genes in Kranz regulation, and has provided insight into the complex regulation underpinning Kranz development in maize.
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Sawers, Ruairidh J. H. "Functional analysis of bundle sheath defective2." Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342541.
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Saropulos, Athanassios S. "Chilling-induced physiological dysfunction in leaves of Zea mays L. and Capsicum annuum L. seedlings." Thesis, University of Reading, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283693.
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Jezek, Mareike [Verfasser]. "Magnesium deficiency in maize and effectiveness of nutrient supply through MgSO4 leaf-application / Mareike Jezek." Kiel : Universitätsbibliothek Kiel, 2016. http://d-nb.info/1111558647/34.
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Khethisa, Joang Adolf. "A highly accessible application for detection and classification of maize foliar diseases from leaf images." Master's thesis, University of Cape Town, 2017. http://hdl.handle.net/11427/25359.
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Crop diseases are a major impediment to food security in the developing world. The development of cheap and accurate crop diagnosis software would thus be of great benefit to the farming community. A number of previous studies, utilizing computer vision and machine-learning algorithms, have successfully developed applications that can diagnose crop diseases. However, these studies have primarily focussed either on developing large scale remote sensing applications more suited for large scale farming or on developing desktop/laptop applications and a few others on developing high end smartphone applications. Unfortunately, the attendant hardware requirements and expenses make them inaccessible to the majority of the subsistence farmers, especially those in sub-Saharan Africa where both smartphones and personal computers ownership is minimal. The primary objective of our research was to establish the feasibility of utilizing computer vision and machine learning techniques to develop a crop diseases diagnosis application that is not only accessible through personal computers and smartphones but is also accessible through any internet enabled feature phone. Leveraging methods established in previous papers, we successfully developed a prototype crop diseases diagnosis application capable of diagnosing two maize foliar diseases, Common Rust and Grey Leaf Spot. This application is accessible through personal computers and high end smartphones as well as through any internet enabled feature phones. The solution is a responsive web based application constructed using open source libraries whose diagnosing engine utilizes an SVM classifier that can be trained using either SIFT or SURF features. The solution was evaluated to establish classification accuracy, page load times when accessed from different networks and its cross-browser support. The system achieved 73.3% overall accuracy rate when tested using images identical to images end users would upload. Page load times were considerably long on GPRS and 2G network tests. However, they were comparable to average page load times users would experience when accessing google search engine pages from similar networks. Cross-browser support tests indicated that the system is fully compatible with all popular mobile and desktop browsers. Based on the evaluation results, we concluded that it is feasible to develop a crop diseases diagnosis application that in addition to being accessible through personal computers and smartphones can also be accessed through any internet enabled feature phones.
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Kinyua, Zakayo Murimi. "Genetic structure, virulence chracteristics and survival of 'Cercospora' populations causing maize grey leaf spot in Kenya." Thesis, Royal Holloway, University of London, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.407993.
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Kakumanu, Akshay. "Effects of Drought on Gene Expression in Maize Reproductive and Leaf Meristem Tissues as Revealed by Deep Sequencing." Thesis, Virginia Tech, 2012. http://hdl.handle.net/10919/33907.
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Drought is a major environmental stress factor that poses a serious threat to food security. The effects of drought on early reproductive tissue at 1-2 DAP (days after pollination) is irreversible in nature and leads to embryo abortion, directly affecting the grain yield production. We developed a working RNA-Seq pipeline to study maize (Zea mays) drought transcriptome sequenced by Illumina GSIIx technology to compare drought treated and well- watered fertilized ovary (1-2DAP) and basal leaf meristem tissue. The pipeline also identified novel splice junctions - splice variants of previously known gene models and potential novel transcription units. An attempt was also made to exploit the data to understand the drought mediated transcriptional events (e.g. alternative splicing). Gene Ontology (GO) enrichment analysis revealed massive down-regulation of cell division and cell cycle genes in the drought stressed ovary only. Among GO categories related to carbohydrate metabolism, changes in starch and sucrose metabolism-related genes occurred in the ovary, consistent with a decrease in starch levels, and in sucrose transporter function, with no comparable changes occurring in the leaf meristem. ABA-related processes responded positively, but only in the ovaries. GO enrichment analysis also suggested differential responses to drought between the two tissues in categories such as oxidative stress-related and cell cycle events. The data are discussed in the context of the susceptibility of maize kernel to drought stress leading to embryo abortion, and the relative robustness of actively dividing vegetative tissue taken at the same time from the same plant subjected to the same conditions. A hypothesis is formulated, proposing drought-mediated intersecting effects on the expression of invertase genes, glucose signaling (hexokinase 1-dependent and independent), ABA-dependent and independent signaling, antioxidant responses, PCD, phospholipase C effects, and cell cycle related processes.
This work was supported by the National Science Foundation Plant Genome Research Pro- gram (grant no. DBI0922747), iPlant Collaborative (NSF DBI-0735191) and also NSF ABI1062472.Master of Science in Life Sciences
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Das, Biswanath. "Epidemiology and management of cercospora zeae-maydis, casual pathogen of gray leaf spot of maize, in Zambia." Thesis, Imperial College London, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.498197.
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Gordon, Stuart G. "Genetic Mapping and Components of Resistance to Cercospora Zeae-Maydis in Maize." The Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=osu1041605948.
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Ntuli, Jean Felistas. "Characterisation of phytoalexin accumulation in maize inoculated with Cercospora zeina, the causal organism of grey leaf spot disease." Master's thesis, University of Cape Town, 2016. http://hdl.handle.net/11427/20848.
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Grey Leaf Spot (GLS) is a fungal disease of Zea mays (maize) that is caused by Cercospora zeina. It thrives in sub-tropical climates and causes devastating crop losses of up to 60% in southern Africa where maize is grown as a staple food source. Phytoalexins are low molecular weight anti-microbial bio-chemicals that are synthesised in planta in response to biotic stress. Related studies have characterised many phytoalexins produced in various plants against several diseases. In maize, phytoalexins fall into to two terpenoid groups: kauralexins and zealexins. To date no studies have been carried out that examine the accumulation in maize of phytoalexins in response to C. zeina. This research project found that in maize samples inoculated with C. zeina, kauralexin accumulation significantly increased with disease development stages (T0 - 0 days post inoculation, T1 - 17 dpi, T2 - 18 dpi and T3 - 24 dpi) while zealexins did not change. Gene expression of the phytoalexin biosynthesis genes TPS6 and TPS11 (both encoding the protein terpene synthase 6/11, specific for zealexins) and CPPS2 (encoding ent-copalyl diphosphate synthase 2, specific for kauralexins) increased significantly at each time point, reaching a maximum level at T2. Infiltration of maize leaves with a chitosan elicitor to mimic fungal pathogen associated molecular pattern (PAMP), and a subsequent callose assay showed positive induction of a callose defence response. However, gene expression and phytoalexin accumulation did not change following chitosan treatment, although zealexin accumulation was higher than kauralexins. Previous studies have shown that phytoalexins accumulate transiently in seedlings. Six diverse Southern African maize lines were compared for phytoalexin accumulation at seedling stage. Zealexin accumulation was generally higher than kauralexins and there were significant differences in both zealexin and kauralexin accumulation in different lines. Gene expression analysis using Genevestigator looked at microarray files and found that expression of TPS6/11 (zealexin biosynthesis) and CPPS2 (kauralexin biosynthesis) genes to be largely co-regulated and highly expressed in response to fungal pathogens, nematodes, insect pests and abiotic stresses; Ustilago maydis, Phytophthora cinnamomi, Fusarium moniliforme, Colletotrichum graminicola, Sporisorium reilianum, Meloidogyne incognita, Ostrinia nubilalis, waterlogging and drought stress. Finally promoter region analysis showed similar cis-acting regulatory elements in the 1kb region upstream of the promoter of both genes and defence specific elements. Thus kauralexin phytoalexins are produced in response to C. zeina inoculation, chitin is not likely to be the key PAMP leading to phytoalexin accumulation, phytoalexin accumulation in seedlings is genotype-dependent and phytoalexin biosynthesis genes are expressed under different conditions suggesting a wider range of action beyond repelling fungal pathogens.
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Kiula, Barnabas Anthony. "Increasing line combining ability and gray leaf spot resistance in maize by integrating conventional breeding with DNA marker technology." Pretoria : [s.n.], 2007. http://upetd.up.ac.za/thesis/available/etd-07282008-134757.
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Galiano, Carneiro Ana Luísa [Verfasser], and Thomas [Akademischer Betreuer] Miedaner. "Genomics-assisted breeding strategies for quantitative resistances to Northern corn leaf blight in maize (Zea mays L.) and Fusarium diseases in maize and in triticale (× Triticosecale Wittm.) / Ana Luísa Galiano Carneiro ; Betreuer: Thomas Miedaner." Hohenheim : Kommunikations-, Informations- und Medienzentrum der Universität Hohenheim, 2021. http://d-nb.info/123663019X/34.
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Edoka, Patrick Nixon. "Influence of leaf area development of early and mid-early maturity varieties of silage maize on dry matter yield and forage quality." Doctoral thesis, Humboldt-Universität zu Berlin, Landwirtschaftlich-Gärtnerische Fakultät, 2006. http://dx.doi.org/10.18452/15441.
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Kenntnisse zur Blattflächenentwicklung von Silomaissorten während der Vegetationsperiode sind erforderlich, um die Ausreife der Pflanzen charakterisieren und neue Sorte bewerten zu können. Die Blattfläche ist eine Funktion von Blattzahl und Blattfläche und kann den Ertrag und die Futterqualität von Silomais in Abhängigkeit von den Umweltbedingungen in unterschiedlichem Ausmaß variieren. Ein maßgebliches Kriterium für das Erreichen einer guten Futterqualität ist die Prognose des optimalen Erntetermins. In den Jahren 2002 und 2003 wurden zwei Experimente am Standort Berge des Institutes für Pflanzenbauwissenschaften (Landwirtschaftlich-Gärtnerische Fakultät der Humboldt-Universität zu Berlin) durchgeführt, um zu zeigen, wie sich Silomaissorten der Reifegruppen früh und mittelfrüh im Blattflächenindex, in der Blattentwicklung sowie spezifischen Blattfläche unterscheiden und welche Unterschiede zwischen zwei Messmethoden zur Bestimmung des Blattflächenindexes bestehen. Unter Beachtung von Ertrag und Futterqualität haben sich bei limitiertem Wasserangebot unter den gegebenen Standortbedingungen Sorten mit einer geringeren Anzahl von Blattgenerationen (13 bis 16) als geeignet erwiesen. Um Trockenmassegehalte in der Gesamtpflanze im optimalen Bereich von 30 bis 35 % im Erntegut garantieren zu können, sollte Silomais speziell unter trocken-heißen Abreifebedingungen dann geerntet werden, wenn mindestens zwei Blätter unterhalb des Kolbenansatzes noch grün sind.Knowledge of leaf area development of silage maize varieties during the vegetation period is useful in the characterisation of the maturity conditions of plants and in the evaluation of new varieties. Leaf area, which is a function of leaf number and leaf size may affect yield and quality parameters of silage maize at varying levels, depending on the environmental conditions under which the crops are grown. One of the criteria for obtaining good quality forage is prognosis for optimum harvest time. Two experiments were conducted in 2002 and 2003 at Berge research station, belonging to the Institute of Crop Science (Faculty of Agriculture and Horticulture, Humboldt-University Berlin) with the aim to assess how silage maize varieties of maturity group early and mid early differ in LAI, leaf area development, specific leaf area, what differences exist between the two methods used to measure LAI. Considering yield and forage quality, under the condition of location Berge, with limited water availability, varieties with fewer leaves (13-16) may be suitable. To maintain the whole plant dry matter content within the optimum range (30-35%), especially under drought condition, harvest time must fall within the period when at least a minimum of two leaves below the cob leaf are still green.
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Perduns, Renke [Verfasser]. "The role of histone modifications in gene regulation : from C4 genes to a genome-wide exploration along the maize leaf gradient / Renke Perduns." Hannover : Technische Informationsbibliothek (TIB), 2016. http://d-nb.info/1107035236/34.
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Dignat, Grégoire. "Dissection génétique de la croissance foliaire et de ses composantes écophysiologiques chez le maïs." Thesis, Montpellier, SupAgro, 2012. http://www.theses.fr/2012NSAM0041.
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L'objectif de cette thèse était d'analyser le déterminisme génétique de la croissance foliaire (LER) du maïs (Zea mays L.). Nous avons combiné plusieurs approches visant à (i) résumer l'information génétique tirée de trois populations de cartographie de QTL (une d'origine tropicale et deux tempérées), (ii) tester l'effet de l'introgresssion de diversité allélique dans les QTL les plus prometteurs, (iii) évaluer jusqu'à quel point les QTL de croissance foliaire affectent la croissance d'autres organes de la plante (iv) disséquer des QTLs d'intérêt par cartographie fine ou génétique d'association locale. La première partie de ce travail concerne le déterminisme génétique de la croissance foliaire maximale (LERmax) évaluée dans des conditions optimales la nuit. LERmax, telle que mesurée en plateforme de phénotypage, partage dans une forte proportion, son contrôle génétique avec la croissance d'autres organes. Des QTL qui affectaient LERmax ou/et la croissance d'autres organes ont alors été disséqués. Une région génomique a été cartographiée avec 23 lignées quasi-isogéniques (NILs) séquentiellement introgressées dans les bins 1.10-11, réduisant ainsi l'intervalle de confiance du QTL d'un facteur 3. Une seconde région génomique a été analysée par une méthode innovante fondée sur une étude d'association ciblée sur une région génomique dans un série allélique générée par introgression de 62 allèles donneurs tirés des lignées parentales d'hybrides cultivés et de populations historiques de maïs d'Amérique Latine dans une lignée élite. L'étude d'association dans cette région relativement petite révèle plus de polymorphismes causaux qu'attendus (six SNP en faible déséquilibre de liaison vs trois QTL consensus).La seconde partie de ce travail considère la sensibilité de la croissance foliaire à la demande évaporative et au déficit hydrique du sol. Un détermisme génétique commun aux deux sensibilités a été mis en évidence par méta-analyse de QTLs initialement détectés dans trois populations en ségrégation et par le test de NILs. Huit métaQTL situés dans quatre régions génomiques ont été testés avec 6 à 17 allèles introgressés pour identifier des NILs qui manifestaient les plus forts effets sur le phénotype. Nous avons initié une cartographie fine dans une de ces régions génomiques à partir d'une populations de recombinants issues de l'introgresssion d'un donneur d'origine tropicale dans B73The objective of this thesis was to analyze the genetic control of the Leaf Elongation Rate (LER) of maize (Zea mays L.). We combined approaches that (i) summarize the QTL information of three mapping populations (one tropical, two temperate), (ii) tested the impact of the introgression of allelic diversity at most promising QTLs, (iii) test to what extent QTLs of LER affect different traits (iv) dissect QTLs of interest by fine mapping or local association mapping.The first part of this document focuses on the genetic control of maximum LER (LERmax) measured in near-optimal conditions during the night. LERmax, as measured in a phenotyping platform, shares an appreciable proportion of its genetic control with the growth abilities of other organs. QTLs affecting LERmax and/or the growth of other organs were therefore dissected. One genomic region was fine-mapped with 23 Near-Isogenic Lines (NILs), sequentially introgressed in the bins 1.10-11, resulting in a reduction of the confidence interval by a factor 3. A second genomic region was analysed after the development of an innovative method of local association mapping on a collection of NILs, introgressed with 62 donor parents from historical populations from different altitude and latitudes in Latin America. This relatively small region harbors more causal polymorphisms than expected (six associated markers in low linkage disequilibrium vs three cQTLs).The second part focuses on the sensitivities of LER to evaporative demand or to soil water deficit. The two sensitivities share a large part of their genetic control as demonstrated by a metaQTL analysis on three mapping populations and the test of NILs. Eight metaQTLs in four genomic regions were tested with 6 to 17 different alleles to find the NILs that best impact the phenotype. We started a fine mapping on one genomic region by using one population of NILs involving a tropical donor
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Fouracre, Jim P. "Genetic regulation of Kranz anatomy." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:7f10306d-d942-49cd-b12f-35b29311ad3c.
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The C₄ photosynthetic cycle acts to concentrate CO₂ around the enzyme Rubisco. By doing so, C₄ photosynthesis leads to increased radiation, water and nitrogen use efficiencies. As such, C₄ photosynthesis is the most productive form of photosynthesis known. Because it enables such high levels of productivity there are large international efforts to introduce C₄ photosynthesis into non-C₄ crop species such as rice. Kranz anatomy is a characteristic leaf cellular arrangement of concentric rings of bundle sheath and mesophyll cells around closely spaced veins and is crucial to C₄ photosynthesis in almost all known examples. Despite the fact that Kranz has evolved on over 60 times independently little is known about the genetic regulation of Kranz development, as attempts to elucidate Kranz regulators using conventional mutagenesis screens have provided few insights. However, the advent of next generation DNA sequencing technologies has enabled the interrogation of genetic networks at a previously unprecedented scale. The work in this thesis describes a genome-wide transcriptomic analysis of leaf development in maize, a C₄ species, that develops both Kranz-type and non-Kranz-type leaves. Detailed bioinformatics analyses identified candidate regulators of both Kranz development and additional aspects of maize leaf development. Three of the identified Kranz candidates were functionally characterised in both C₄ and non-C₄ species. Furthermore, expression and phylogenetic analyses of GOLDEN2-LIKE (GLK) genes, a small transcription factor family previously implicated in C₄ development in maize, were extended to determine the generality of GLK function in C₄ evolution.
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Solontsi, Mhlangabezi. "The response of maize to selected ratios of organic fertilizers mixed with inorganic fertilizers in Ndlambe Local Municipality, Eastern Cape, South Africa." Thesis, Nelson Mandela Metropolitan University, 2013. http://hdl.handle.net/10948/d1020902.
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The majority of small-scale farmers in the Eastern Cape apply inadequate quantities of manure, inorganic (NPK) fertilizer and Gromor as lone fertilizers or tend to mix manure or Gromor with chemical fertilizers for crops, without any scientific basis (Mkile, 2001). Hence this study – aimed at achieving effective and efficient use of chemical fertilizers, manure and Gromor, either as lone fertilizers or mixed with one another, in maize production – was established. A three-year dry land field experiment, arranged in a RCBD with 3 replicates where cattle manure, chemical fertilizer and Gromor were mixed at different ratios – as treatments FM1 (10 percent fertilizer + 90 percent manure); FM2 (25 percent fertilizer + 75 percent manure); FM3 (50 percent fertilizer + 50 percent manure); MAP (100 percent fertilizer); M (100 percent cattle manure); FG1 (10 percent fertilizer + 90 percent Gromor); FG2 (25 percent fertilizer + 75 percent Gromor); FG3 (50 percent fertilizer + 50 percent Gromor); G (100 percent Gromor) and a control (with nothing applied) – was conducted at the Nolukhanyo agricultural project at Bathurst in the Ndlambe Local Municipality. Maize cultivars PAN 6480 and Sahara were used as test crops. Manure, Gromor and soil were analyzed for their nutrient composition. Data recorded involving number of kernels per cob-1, grain and dry matter (DM) yields for the 2010-11, 2011-12 and 2012-13 seasons, and leaf area index (LAI) – were subjected to Genstat Version 14.1 for statistical analysis. Grain yields of PAN 6480 and Sahara recorded in seasons 2010-11, 2011-12 and 2012-13 were significantly higher (p≤0.05) than those of the controls. In 2012-13, PAN 6480 on treatments FG3 and FG1 produced the highest grain yields (5 327 and 5 107kg ha-1) respectively, while in 2010-11 the highest yield was 3 780kg ha-1. In the 2012-13 season, Sahara on F treatment was the highest with 4 553kg ha-1 whilst there were no significant differences in F and M treatments. Dry matter yields of Sahara were higher than those of PAN 6480 in all seasons. The highest Sahara LAI obtained on FM2 was 5.85 in the 2012-13 season, whereas PAN 6480 had LAI of 4.77 on F treatment, followed by FG3 with 4.72 LAI. PAN 6480 number of leaves for 6 plants in m2 and LAI in week 8 of the 2012-13 season had a positive correlation, r2=0.84. PAN 6480 number of kernels and cob length in the same area correlated quite well (r2 =0.91). Chemical fertilizer corrects the imbalances of nutrients inherent in organic manures and increases both maize grain yields and dry matter yields, and also influences number of leaves and LAI. Treatments FG1, FG3 and FM1 of PAN 6480 resulted in high maize grain yields when planted 13 in late November of the 2012-13 growing season and when water in the soil profile was conserved as a result of good rains received prior to planting. Treatments FG1 (10 percent fertilizer + 90 percent Gromor), FG3 (50 percent fertilizer + 50 percent Gromor) of PAN 6480 produced the highest grain yields (5 107kg ha-1 and 5 327kg ha-1 respectively) in the 2012-13 season, whilst FM1 (10 percent fertilizer + 90 percent manure) gave the highest grain yield (of 4 277kg ha-1) for the same cultivar in the 2010-11 season.
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Uddin, Md Nesar [Verfasser]. "Effect of salinity in the first phase of salt stress on leaf cell-wall components of maize with special reference to cell-wall extensibility / Md. Nesar Uddin." Gießen : Universitätsbibliothek, 2012. http://d-nb.info/1064838774/34.
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Kgasago, Hans. "Effect of planting dates and densities on yield and yield components of short and ultra-short growth period maize (Zea mays L.)." Diss., University of Pretoria, 2007. http://hdl.handle.net/2263/28056.
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In general, yield reduction in most dryland maize growing areas of South Africa occur because seasonal rainfall distribution is erratic with annual variation that cannot be predicted accurately. Cultivar selection, planting date and plant density are other factors that consistently affect maize yield. Long growing season maize cultivars are higher yielding, particularly under conditions of good moisture and nutrient supply. However, as both moisture and nutrient availability becomes more limiting, yield tends to decline. Short growing season maize cultivars could yield more than long season counterparts because they can maximize the growing season and potentially reach the critical flowering stage before traditional midsummer droughts occur. The short growing season maize cultivars, which have only recently been developed, have traits, which can address the problem of reduced yield, which is ascribed to midsummer drought. There has been no previous effort to evaluate the effects of planting dates and plant densities on yield and yield components of these short and ultra-short growth period maize cultivars. This prompted research in the 2004/05 growing season. One field experiment was conducted at each of two selected areas (Bethlehem&Potchefstroom) in the “Maize Triangle” of South Africa. The aim was to evaluate the response of short and ultra-short growth period maize cultivars to planting dates and plant densities at two localities with distinct environmental conditions. The effects of planting date, plant density and cultivar on yield and yield components were investigated. Both yield and yield components were affected by planting date, plant density and cultivar at both localities. At both localities early and optimum planting dates as well as low and optimum plant densities promoted increases in yield components, which contributed to increased grain yield. As for the cultivars, PAN6017 proved to be the most consistent since it out-performed other cultivars in terms of both vegetative growth, yield components and grain yield at both localities. At both localities, plant height, leaf area index and dry matter yield were affected by both planting date and plant density, with optimum planting date and optimum plant density contributing to highest yield components and yield. PAN 6017 was superior to the other cultivars at all planting dates and plant densities at both localities. In order to make findings from a study such as this applicable to the “Maize Triangle”, more research on short and ultra-short growth period maize cultivars should be conducted over a wider range of locations and seasons.Dissertation (M Inst Agrar (Agronomy))--University of Pretoria, 2007.
Plant Production and Soil Science
M Inst Agrar
unrestricted
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Mohamed, Abdel Kareem Sayed Hussein [Verfasser]. "The role of Na+/H+ antiporter activity in leaf cells of newly developed maize (Zea mays L.) hybrids for sodium inclusion and salt resistance / Abdel Kareem Sayed Hussein Mohamed." Gießen : Universitätsbibliothek, 2011. http://d-nb.info/1063111668/34.
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Edoka, Patrick Nixon [Verfasser], Frank [Gutachter] Ellmer, and Karlheinz [Gutachter] Richter. "Influence of leaf area development of early and mid-early maturity varieties of silage maize on dry matter yield and forage quality / Patrick Nixon Edoka ; Gutachter: Frank Ellmer, Karlheinz Richter." Berlin : Humboldt-Universität zu Berlin, 2006. http://d-nb.info/1208074512/34.
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Almeida, Bruno Marçal de. "Déficit e excesso hídrico na cultura do milho (Zea mays L.) em ambiente protegido." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11152/tde-28042016-113144/.
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O milho é uma das culturas de grande importância no cenário agrícola mundial devido sua importante participação no setor alimentício, bem como suas exigências hídricas no decorrer do seu ciclo. Sendo assim, tornam-se importantes os estudos quanto a utilização dos recursos hídricos, onde as estratégias de irrigação são de grande importância para a economia de água. O objetivo do presente estudo foi o de avaliar diferentes lâminas de irrigação (déficit e excesso) no decorrer do ciclo total e em fases fenológicas especificas da cultura do milho. Foram avaliadas as características de desenvolvimento, produção e estado hídrico da planta. Os experimentos foram realizados no Departamento de Engenharia de Biossistemas na Escola Superior de Agricultura \"Luiz de Queiroz\", ESALQ-USP, localizada em Piracicaba - SP. O delineamento experimental foi em blocos casualizados, com 12 tratamentos e quatro repetições. Os tratamentos consistiam na reposição de 150, 100, 50 e 30% da água evapotranspirada (ETc), aplicadas em quatro subperíodos do ciclo total da cultura: subperíodo 1 (V4 a V8), subperíodo 2 (V8 a Vp), subperíodo 3 (Vp a R1) e subperíodo 4 (R1 a R6). Os dados de cada experimento foram submetidos a análise de variância individual e quando possível, análise de variância conjunta, utilizando os dados médios dos experimentos. O 1° plantio foi realizado em 18 de maio de 2012 e o 2° 21 de abril de 2013 em ambiente protegido, totalizando 137 e 144 dias de ciclo, com soma-térmica de 1413 e 1444°C graus dias acumulados (GDA), respectivamente. Avaliou-se a altura das plantas (ALT), altura de inserção da espiga (AIT.I), número de espigas por planta (N.E), peso da espiga com palha (PEP), peso da espiga despalhada (PED), número de grão por espiga (NG), número de fileiras de grão por espiga (NFG), tamanho da espiga (TE), diâmetro da espiga (DE) diâmetro do sabugo (DS), número de entrenós (NE) e produtividade (PG). Mediu-se a temperatura foliar (Tf), déficit de pressão e vapor (DPV) e potencial hídrico foliar(-Ψf). O potencial hídrico foliar mostrou ser o método eficaz quanto ao estado hídrico da planta, havendo diferenciação entre os quatro tratamentos estudados (IIII, EEEE, D50 e D70), demonstrando variação do seu valor ao longo do dia. Calculou-se o coeficiente de resposta da cultura ao déficit hídrico (KY), onde o período de pendoamento e floração foram os mais críticos quando a deficiência hídrica foi imposta em fases fenológicas especificas. A partir dos dados de produtividade e lâminas de água aplicadas nos dois experimentos, foi possível ajustar modelos polinomiais de 2° grau para a produtividade da água (PA) e função de produção da cultura. Os resultados permitiram concluir que a maior PA foi obtida no tratamento D50.Deficit and water excess in maize (Zea mays L.) in greenhouse Maize is a culture with importance in global scenario due your function in food industry, as well as yours water requirements in the course of your cycle. Therefore, studies became important regarding the use of water resources where irrigation strategies are of great importance for water savings. This study aimed evaluate different irrigation levels (deficit and water excess) during a total maize cycle and at different specifics phenological stages of the culture. Development characteristics, production and hydric state of the plant were evaluated. The experiments were conducted at Department of Biosystems Engineering from \"Luiz de Queiroz\" College of Agriculture, located at Piracicaba, São Paulo State. The experimental design was a randomized block, with 12 treatments and 4 repetitions. Treatments consisted in replacement of 150, 100, 50 and 30% of water evapotranspired (ETc), applied in four subperiods of the total culture cycle: subperiod 1 (V4 to V8), subperiod 2 (V8 to Vp), subperiod 3 (Vp to R1) and subperiod 4 (R1 to R6). The experimental data were submitted to individual variance analysis, and when were possible, conjunct variance analysis, using the average data of the experiments. The first planting was carried out on 18 May of 2012 and the second, on April 21 of 2013, in greenhouse, totaling 137 and 144-day cycle, with sum-Thermal 1413 and 1444 °C accumulated degree days (ADD),respectively. Were evaluated the plant height (PH); ear height insertion (AIT.I); number of ears per plant (NE); ear weight with straw (PEP); weight of ear without straw (PED); number of grain per ear (NG); number of grain rows per ear (NFG); ear size (TE); ear diameter (dE); diameter of the cob (DS); number of between-nodes (NE) and productivity (PG). The leaf temperature was measured (Tm), also the vapor pressure deficit (VPD) and leaf water potential (Pl). Leaf water potential showed to be an efficient method regarding hydric state of the plant, there were difference between the four treatments studied (III, EEEE, D50 e D70), demonstrating variation of its value over the day. the response coefficient of the crop to water deficit (KY) were calculated, where the period of bolting and flowering were the most critical when water stress was imposed in specific phenological stages. From the productivity data and water levels applied in both experiments, was possible adjust second degree polynomials models to water productivity (WP) and culture production function. The results allowed conclude that a higher WP were obtained in the treatment D50.
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Brunelli, Kátia Regiane. "Cercospora zeae-maydis: esporulação, diversidade morfo-genética e reação de linhagens de milho." Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/11/11135/tde-13122004-085408/.
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A incidência e severidade da mancha de cercospora, causada por Cercospora zeae-maydis Tehon & Daniels, aumentou significativamente em território brasileiro a partir do ano 2000, sendo hoje considerada uma das principais doenças foliares da cultura do milho. Mesmo assim, poucos estudos com este patossistema foram realizados no Brasil. Este trabalho teve por objetivo determinar meio de cultura e regime luminoso para adequada esporulação de C. zeae-maydis, estudar a reação de um grupo de 118 linhagens endogâmicas de milho quanto a resistência ao patógeno em dois ambientes distintos (Indianópolis-MG e Jardinópolis-SP), observar aspectos microscópicos da esporulação, germinação e penetração em hospedeira suscetível e avaliar diferenças morfológicas, genéticas e de agressividade entre isolados coletados na região centro-sul do país. Os resultados indicaram que a melhor esporulação do fungo foi obtida em meio V8 e suco de tomate temperado quando submetidos a fotoperíodo 12/12h (luz/escuro). Quanto a reação das linhagens à doença, foi possível verificar interação diferencial significativa entre genótipo de milho e os dois ambientes, indicando que fatores ambientais ou patogênicos, distintos entre os locais, podem ter contribuído para os discrepantes comportamentos de alguns genótipos. Também foi possível verificar elevado nível de resistência em 12 linhagens em ambos locais, demonstrando a existência de genótipos mais estáveis para resistência com possibilidade de uso em programas de melhoramento da cultura. Através da análise do padrão de restrição gerado pela digestão da região ITS-5.8S do rDNA, de 104 locos AFLP e de mensurações morfométricas dos conídios, foi possível verificar a existência de dois grupos geneticamente distintos de C. zeae-maydis em território brasileiro. Estes são relatados na literatura como grupos I e II ou espécies afins (siblings species). Estes grupos foram detectados em todos os locais de coleta do território brasileiro, com exceção de Goiás, onde o grupo I não foi observado. Quanto aos aspectos microscópicos deste patógeno, foi possível verificar que sob condições ambientais adequadas a germinação dos esporos ocorre 13 horas após o contato do esporo com a hospedeira, e a penetração, via estômato, tem início 16 horas após a inoculação. Também foi observado o fenômeno da conidiação microcíclica nos isolados brasileiros. Vinte e seis por cento daqueles pertencentes ao grupo I produziram microconídios, enquanto nenhum do grupo II apresentou esta característica. Deste modo, este é o primeiro relato da existência deste fenômeno no grupo I e ausência no grupo II. Estes estudos demonstram que a população brasileira de C. zeae-maydis se assemelha àquelas existentes nos Estados Unidos e na África, com a prevalência dos dois grupos genéticos.The incidence and severity of cercospora leaf spot, caused by Cercospora zeae-maydis Tehon & Daniels, increased significantly in Brazil in 2000, being considered today one of the major leaf disease of the crop. Despite this, few researches about the pathosystem come being carried in Brazil. The aims of this work were to identify the suitable culture media and light conditions for sporulation of C. zeae-maydis; to study the reaction of 118 mayze genotypes to pathogen in two different locations (Indianópolis Minas gerais State and Jardinópolis São Paulo State); to observe some microscopical aspects of esporulation, germination and penetration in a susceptible maize genotype; and finally to assess morphological and genetic differences among a group of isolates collected in center-south Brazil. The results showed that the better culture media for esporulation was the V8 media and tomato juice, under 12-hours photoperiod. Concerning to genotype reaction to disease, it was possible to verify significant interaction between genotypes and environment, indicanting that environmental or pathogenic factors, distinct between locations, may have influenced the reactions of some genotypes. It was possible to identify highly level of resistance in 12 lines in both places, evidencing the existence of stable genotypes that can be used in breeding programs. Analysis of restriction fragments from ITS-5.8S of rDNA, 104 AFLP loci, and conidial measurements, showed the existence of two genetically divergent groups of C. zeae-maydis in Brazil. These groups are similar to the ones reported previously reported as I and II groups or siblings species. Both groups were detected in all sampled regions, except Goiás State where no isolates from group I were detected. Concerning to microscopic traits, it was possible to verify that the brazilian isolates of this pathogen have the ability for production of microconidia. Twenty six percent of the isolates of the group I produced microconidia, while none of the group II showed this trait. Thus, this is the first report with presence of MC in the group I but absence in the group II. The results showed that Brazilian isolates are very similar to isolates from USA and Africa, occurring both genetic groups.
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Mathioni, Sandra Marisa. "Agressividade de isolados de Cercospora zeae-maydis em genótipos de milho." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-05072006-103641/.
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A cultura do milho (Zea mays L.) apresenta grande importância cultural, social e econômica, tanto no Brasil como no mundo. Entre os fatores que contribuem grandemente para a diminuição de sua produtividade estão as doenças. A mancha de cercospora ou cercosporiose, causada pelo fungo Cercospora zeae-maydis Tehon & Daniels, é uma das principais doenças da cultura em vários países. Uma vez que o uso de híbridos resistentes é a medida mais eficiente para o seu controle, a caracterização e a discriminação de genótipos de milho quanto ao nível de resistência e de isolados do patógeno quanto ao nível de agressividade é uma etapa fundamental de qualquer programa de melhoramento. Isolados de C. zeae-maydis podem ser classificados em dois grupos genéticos, denominados I e II, segundo análise por marcadores AFLP e por restrição da região intergênica espaçadora do rDNA 5.8S. No Brasil, há ocorrência dos dois grupos, mas nos Estados Unidos há uma prevalência do grupo I sobre o grupo II, ao passo que em países do sul da África verifica-se somente a presença de indivíduos do grupo II. Presentemente, não há nenhum relato sistemático sobre diferenças em agressividade entre indivíduos destes grupos. Assim, o objetivo deste trabalho foi avaliar a agressividade de 20 isolados de C. zeae-maydis dos grupos genéticos I e II quando inoculados em um híbrido de milho suscetível e também testar o comportamento de 10 linhagens de milho frente a oito isolados em dois ambientes para verificar a possível presença de interação diferencial entre isolado, linhagem e local. Para tanto, os isolados foram cultivados em sementes de sorgo para produção de inóculo. Sementes colonizadas foram depositadas no cartucho das plantas e as avaliações foram realizadas utilizando-se escalas diagramáticas. No experimento em casa de vegetação foi verificada uma variação em agressividade entre os isolados do grupo genético I, do grupo genético II e entre os grupos. Observou-se ainda que isolados pertencentes ao grupo genético II são, em média, mais agressivos que isolados do grupo genético I. Este é o primeiro relato de diferenças em agressividade entre os grupos genéticos. No experimento em campo não foram observadas diferenças significativas quanto à agressividade dos isolados avaliados em Jardinópolis (SP) e Indianópolis (MG). Entretanto, observou-se forte interação entre linhagens e locais, indicando que o ambiente exerce influência na doença. Dessa forma, recomendam-se avaliações em ambientes diferentes e a utilização de isolados mais agressivos e pertencentes aos dois grupos genéticos a fim de otimizar a discriminação de genótipos de milho com relação à resistência a C. zeae-maydis.Maize (Zea maydis L.) is of great social, cultural and economical importance in Brazil and in the world. Maize diseases are the main factors that reduce crop yield. Gray leaf spot, caused by the fungus Cercospora zeae-maydis Tehon & Daniels, is one of the most important diseases in many countries. Given that the disease can be controlled by the use of resistant maize hybrids, the characterization and discrimination of maize genotypes according to their level of resistance and of pathogen isolates according to their aggressiveness are fundamental steps in any breeding program. Isolates of C. zeae-maydis can be classified into two genetic groups, named I and II, by analysis with AFLP markers and restriction of the intergenic spacer region of the 5.8S rDNA. In Brazil, both groups occur whereas in the United States isolates from group I prevail and in some South African countries only isolates from group II can be found. Presently, there are no systematic reports on differences in aggressiveness between these groups. Therefore, the objective of this study was to evaluate the aggressiveness of 20 isolates of C. zeae-maydis from both genetic groups when inoculated in a susceptible maize hybrid and also to test the reaction of 10 maize inbreds to eight C. zeae-maydis isolates in two environments in order to assess the possible occurrence of differential interaction between isolates, inbreds, and locations. Isolates were cultivated in sorghum seeds for innoculum production. Colonized seeds were placed into the whorl of the plants and the disease reaction was evaluated using a diagrammatic scale. In the greenhouse experiment significant variation in aggressiveness was observed among isolates of group I, group II and between groups. Also, it was observed that isolates from group II were, on average, more aggressive than isolates from group I. This is the first report on differences in aggressiveness between the two genetic groups of C. zeae-maydis. In the field experiment no significant differences were observed in aggressiveness among isolates evaluated in Jardinópolis (SP) and Indianópolis (MG) for the inbreds tested. However, a strong interaction between reactions of maize inbreds and regions was observed indicating that the environment influences the disease. Thus, evalutions in several locations with aggressive isolates from both groups is recommended in order to optimize the discrimination of maize genotypes regarding their resistance to C. zeae-maydis.
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Soares, Marcio Augusto. "Influência de nitrogênio, zinco e boro e de suas respectivas interações no desempenho da cultura de milho (Zea mays L.)." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-20102003-165553/.
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O presente trabalho teve como objetivo avaliar o modo como os micronutrientes zinco e boro e suas interações com a adubação nitrogenada influenciam no desempenho da cultura do milho (Zea mays L.). O experimento foi conduzido em área pertencente à Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), no município de Piracicaba-SP, no ano agrícola de 2000/2001, em solo classificado como Nitossolo Vermelho, Eutrófico, onde foi semeado o híbrido Cargill 909. O delineamento experimental utilizado foi casualizado em blocos com vinte e sete tratamentos e três repetições, onde tanto o zinco como o boro foram aplicados de forma localizada no sulco, por ocasião da semeadura do milho. Os tratamentos corresponderam a cinco doses de zinco (0, 2, 4, 8 e 16 kg.ha -1 ) e cinco doses de boro (0, 1, 2, 4 e 8 kg.ha -1 ), associados ou não ao nitrogênio, o qual foi aplicado em três doses (0, 120 e 240 kg.ha -1 ). Cabe salientar que o nitrogênio foi aplicado parceladamente na forma de uréia (45% N), sendo 30 kg.ha -1 aplicados no sulco de semeadura e o complemento, específico de cada tratamento, em cobertura, no momento em que a cultura de milho apresentava cinco folhas plenamente expandidas. Como fontes de zinco e de boro, foram utilizados o sulfato de zinco (20% Zn) e o ácido bórico (17% B), respectivamente. Todas as parcelas receberam, no sulco de semeadura, 80 kg.ha -1 de P2O5, na forma de superfosfato simples, e 60 kg.ha -1 de K2O, na forma de cloreto de potássio. Visando avaliar especificamente a influência dos referidos nutrientes nas plantas de milho, as parcelas experimentais foram mantidas totalmente livre da presença de pragas, doenças e plantas daninhas durante todo o ciclo da cultura. Quanto às avaliações, foram determinados o índice de área foliar, o número de folhas fotossinteticamente ativas por planta, a altura de planta, a altura de inserção da espiga, o diâmetro do colmo, o comprimento do primeiro e do segundo internódio do colmo e a massa de matéria seca de folhas, colmo, pendão e espiga. Ainda, foram determinados os componentes de produção como o número de espigas por planta (prolificidade), o comprimento da espiga, o número de grãos por fileira, o número de fileiras de grãos na espiga, o comprimento dos grãos, a massa de mil grãos e a produtividade de grãos. De acordo com os resultados obtidos, concluiu-se que a aplicação de nitrogênio em doses crescentes proporcionou aumento tanto da produtividade de grãos, como de uma série de outras variáveis que contribuíram para esse aumento. As variáveis numero de folhas e índices de área foliar aos 20 dias após a emergência, comprimento do primeiro internódio do colmo, massa de matéria seca de pendão, numero de fileiras de grãos e o comprimento dos grãos não apresentaram respostas a nenhum dos nutrientes estudados. A máxima produtividade de grãos (9182 kg.ha -1 ) foi obtida mediante o uso da maior dose de N, sendo essa dose considerada não recomendada devido ao baixo incremento que proporcionou (9%) em relação à aplicação de 120 kg.ha -1 de N. Doses de N maiores que 120 kg.ha -1 não proporcionaram aumento satisfatórios das variáveis estudadas, inclusive da produtividade. Doses elevadas de zinco ( até 16 kg.ha -1 ) e de boro (até 8 kg.ha -1 ) aplicadas no sulco de semeadura não exerceram influência sobre a produtividade de grãos, permitindo concluir que, nas condições em que o trabalho foi desenvolvido, a toxidez provocada pelo excesso de micronutriente é mínimo. A variável que apresentou maior correlação com a produtividade de grãos foi o comprimento de espigas. Houve também alta correlação do numero de folhas com o índice de área foliar.With the purpose of evaluating the nitrogen, zinc and boron and their interactions in the maize crop performance, a field experiment was carried out at Piracicaba, São Paulo State, Brazil (University of São Paulo, ESALQ), during the agricultural year of 2000/2001, where the Typic Kandiudalf soil and Cargill 909 hybrid were used. The experimental design was completely randomized blocks with twenty seven treatments and three replications, where zinc and boron were applied at maize sowing day. The treatments had five zinc doses (0, 2, 4, 8 and 16 kg.ha -1 ) and five boron doses (0, 1, 2, 4 and 8 kg.ha -1 ), associated or not to the nitrogen, which was applied in three doses (0, 120 and 240 kg.ha -1 ). The nitrogen was applied using urea form (45% N), being 30 kg.ha -1 applied at sowing day and the complement, specific for each treatment, when the maize crop presented five leaves completely expanded. The zinc sulphate (20% Zn) and boric acid had been used (17% B) as source of zinc and boron, respectively. All plots had received, at sowing day, 80 kg.ha -1 of P2O5, and 60 kg.ha -1 of K2O (potassium chloride). To evaluate the influence of the nutrient related of maize crop, the experimental area had been kept total free of weeds, pests and diseases during all crop cycle. The following evaluations were done: the maize crop phenological stages, leaf area, spike insertion and plant height, diameter of stem internode (first and second), dry mass of leaves, stem, tassel and spike, number of spikes per plant, mass of spikes with and without straw, mass of thousand grains, spike diameter, grain length, number of grain rows in the spike, spike length, number of grains per row and grains productivity. Accordging to the results, it was possible to verify that the nitrogen application in crescent doses increased the grain productivity and other variables. The following variables did not present relationship with all nutrients studied: number of leaves and leaf area index at twenty days after emergency, length of first internode stem, tassel dry mass, number of grain rows and the grain length. The maximum grain productivity (9182 kg.ha -1 ) was obtained under higher dose of N, where it was not reccomended because the yiled increment was only 9% when compared with 120 kg.ha -1 of N dose. Doses of N higher than 120 kg.ha -1 did not increase all studied variables, including grain productivity. High doses of zinc (up to 16 kg.ha -1 ) and boron (up to 8 kg.ha -1 ) applied at sowing date did not influence the grain productivity, allowing to conclude that, under field experiment condition, the toxicity produced by micronutrient excess is minimum. The spike length was the variable with higher correlation with grain productivity. There was also high correlation between number of leaves and leaf area index.
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Gimenes, Leila. "Estudo fitoquímico de Annona sylvatica (Annonaceae). Avaliação das atividades inseticida e fungicida frente às formigas cortadeiras e seu fungo simbionte e ao gorgulho do milho." Universidade Federal de São Carlos, 2013. https://repositorio.ufscar.br/handle/ufscar/6570.
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Previous issue date: 2013-03-27Universidade Federal de Sao Carlos
The leaf-cutting ants are social species, living in permanent colonies. The genera of the greater importance, Atta (saúvas) and Acromyrmex (quenquéns), are the major plagues of Brazilian agriculture, to cause serious damages by its large and uncontrolled grazing, which leads to serious economic losses for many agrosystems. In the same way, the corn maize weevil, Sitophilus zeamais, belonging to the family Curculionidae, is the major pest species of stored corn. The corn grains are drilled and damaged by larvae and adults, causing numerous qualitative and quantitative losses of corn grains disabling its marketing. As the consequence, the effective control of these plagues is extremely important for agriculture and economy that aims to provide the nacional and international market quality products with high commercial value. In this context, the phytochemistry study of Annona sylvatica provides an alternative way to control these plagues, since that studies of these species showed a wide variety of natural products with promising activities, including, insecticide and fungicide, due to the large presence of acetogenins and lignans as secondary metabolites. This study allowed the isolation of fourteen compounds (four lignans, four acetogenins, three alkaloids and three steroids), however were identified eleven compounds due to the high structural complexity presented for the acetogenins. The identification techniques employed have been using single and two-dimensional spectra of 1H and 13C NMR and Mass Spectrometry (HPLC/NMR; HPLC/ESI-MS/MS e HPLC/APCI-MS/MS). For the biological tests, the ethanolic extract of the seeds stood out as potentially toxic to three bioassays cited above. The leaves and branches dichlorometanic fractions obtained by liquid-liquid partitioning of the extracts were toxic for leaf-cutting ants and their symbiotic fungus. Among the acetogenins isolated, only one of them showed a significant activity against corn maize weevil.
As formigas cortadeiras são espécies sociais, vivendo em colônias permanentes. Os gêneros de maior importância econômica, Atta (saúvas) e Acromyrmex (quenquéns), são as principais pragas da agricultura brasileira, por causarem sérios danos pela sua grande e descontrolada herbivoria, o que gera sérios prejuízos econômicos para muitos agroecossitemas. Nessa mesma vertente, o gorgulho do milho, Sitophilus zeamais, pertencente à família Curculionídea, é a principal espécie praga do milho armazenado. Os grãos são perfurados e danificados pelas larvas e adultos, inutilizando sua comercialização. Como consequência, o controle eficaz de tais pragas é de extrema importância para a agricultura e economia do país que visa oferecer ao mercado nacional e internacional produtos de qualidade e alto valor comercial. Nesse contexto, o estudo fitoquímico de Annona sylvatica fornece uma forma alternativa de controle a estas pragas, uma vez que estudos destas espécies mostraram uma ampla variedade de produtos naturais com atividades promissoras, entre elas, inseticida e fungicida, devido à ampla presença de acetogeninas e lignanas como metabólitos secundários. Este estudo resultou no isolamento de 14 compostos (quatro lignanas, quatro acetogeninas, três alcaloides e três esteroides), no entanto, foram identificados 11 compostos, devido à alta complexidade estrutural apresentada para as acetogeninas. As técnicas de identificação utilizadas foram através dos espectros uni e bidimensionais de RMN 1H e 13C e por Espectrometria de Massas (HPLC/NMR; HPLC/ESI-MS/MS e HPLC/APCI-MS/MS). Para os ensaios biológicos, o extrato etanólico das sementes destacou-se como potencial tóxico frente aos três bioensaios citados acima. As frações diclorometânicas das folhas e ramos obtidas através do particionamento líquido-líquido dos extratos foram tóxicas para as formigas cortadeiras e seu fungo simbionte. Dentre as acetogeninas isoladas, apenas uma apresentou uma atividade significativa frente ao gorgulho do milho.
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DeRose, Robert Justin. "Leaf Area Index - Relative Density Relationships in Even-Aged Abies Balsamea - Picea Rubens Stands in Maine." Fogler Library, University of Maine, 2004. http://www.library.umaine.edu/theses/pdf/DeRoseRJ2004.pdf.
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Jiang, Jingyi. "Retrieving leaf and canopy characteristics from their radiative properties using physically based models : from laboratory to satellite observations Estimation of leaf traits from reflectance measurements: comparison between methods based on vegetation indices and several versions of the PROSPECT model a model of leaf optical properties accounting for the differences between upper and lower faces Speeding up 3D radiative transfer simulations: a physically based approximation of canopy reflectance dependency on wavelength, leaf biochemical composition and soil reflectance Effective GAI for crops is best estimated from reflectance observations as compared to GAI and LAI Optimal learning for GAI and chlorophyll estimation from 1D and 3D radiative transfer model inversion: the case of wheat and maize crops observed by Sentinel2." Thesis, Avignon, 2019. http://www.theses.fr/2019AVIG0708.
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La mesure des caractéristiques des feuilles et du couvert végétal par télédétection est un moyen efficace et non destructif d’effectuer un suivi des cultures, que ce soit pour la prise de décision dans la gestion d’itinéraires techniques an agriculture de précision ou pour le phénotypage au champ pour améliorer l'efficacité de la sélection variétale. Grâce à l’augmentation de la puissance de calcul des machines et à la disponibilité croissante d'images à haute résolution spatiale, les méthodes d’estimation peuvent maintenant bénéficier de simulations plus précises des modèles de transfert radiatif (RT) dans la végétation. L'objectif de ce travail est de proposer et d'évaluer des moyens efficaces pour estimer les caractéristiques des feuilles et du couvert végétal à partir d'observations rapprochées ou de télédétection en utilisant des modèles RT basés sur une description réaliste de la structure des feuilles et du couvert. Au niveau des feuilles, nous avons d'abord évalué la capacité des différentes versions du modèle PROSPECT à estimer des variables biochimiques comme la chlorophylle (Cab), la teneur en eau et en matière sèche. Nous avons ensuite proposé le modèle FASPECT pour décrire les différences de propriétés optiques entre les faces supérieure et inférieure des feuilles en considérant un système à quatre couches. Après avoir étalonné les coefficients d'absorption spécifiques des principaux constituants de la feuille, nous avons validé FASPECT sur 8 jeux de données. Nous avons montré que les spectres de réflectance et de transmittance des deux faces sont simulés avec une très bonne précision, et même meilleure que PROSPECT pour la face supérieure. De même, en mode inverse, les performances d'estimation de la teneur en matière sèche sont considérablement améliorées avec FASPECT par rapport à PROSPECT, et restent du même ordre de grandeur pour la chlorophylle et l’eau. Au niveau du couvert végétal, nous avons utilisé le simulateur de rendu physique réaliste LuxCoreRender pour calculer le transfert radiatif à partir d'une description 3D de l’architecture de la culture. Nous avons d’abord vérifié ses bonnes performances par comparaison aux modèles 3D les plus récents en utilisant ROMC (RAMI On Line Model Checker). Afin d’accélérer les simulations, nous avons développé une méthode qui repose sur l’utilisation d’un nombre limité de propriétés optiques du sol et des feuilles. Pour estimer les variables d'état du couvert végétal (indice de surface verte, GAI, contenu en chlorophylle du couvert (CCC) ou des feuilles (Cab), nous avons ensuite entrainé des algorithmes d’apprentissage automatique à partir de bases de données « culture spécifique » simulées avec LuxCoreRender pour le blé et le maïs et d’une base de données générique simulée avec le modèle 1D PROSAIL de transfert radiatif. Les résultats sur des simulations et sur des données in situ combinés aux images SENTINEL2 ont montré que les algorithmes spécifiques aux cultures surpassent les algorithmes génériques pour les trois variables, en particulier lorsque la structure du couvert s’éloigne de l'hypothèse 1D du milieu turbide, comme dans le cas du maïs où la structure en rang domine pendant toute une partie de la saison de croissanceMeasuring leaf and canopy characteristics from remote sensing acquisitions is an effective and non destructive way to monitor crops both for decision making within the smart agriculture practices or for phenotyping under field conditions to improve the selection efficiency. With the advancement of computer computing power and the increasing availability of high spatial resolution images, retrieval methods can now benefit from more accurate simulations of the Radiative Transfer (RT) models within the vegetation. The objective of this work is to propose and evaluate efficient ways to retrieve leaf and canopy characteristics from close and remote sensing observations by using RT models based on a realistic description of the leaf and canopy structures. At the leaf level, we first evaluated the ability of the different versions of the PROSPECT model to estimate biochemical variables like chlorophyll (Cab), water and dry matter content. We then proposed the FASPECT model to describe the optical properties differences between the upper and lower leaf faces by considering a four-layer system. After calibrating the specific absorption coefficients of the main absorbing material, we validated FASPECT against eight measured ground datasets. We showed that FASPECT simulates accurately the reflectance and transmittance spectra of the two faces and overperforms PROSPECT for the upper face measurements. Moreover, in the inverse mode, the dry matter content estimation is significantly improved with FASPECT as compared to PROSPECT. At the canopy level, we used the physically based and unbiased rendering engine, LuxCoreRender to compute the radiative transfer from a realistic 3D description of the crop structure. We checked its good performances by comparison with the state of the art 3D RT models using the RAMI online model checker. Then, we designed a speed-up method to simulate canopy reflectance from a limited number of soil and leaf optical properties. Based on crop specific databases simulated from LuxCoreRender for wheat and maize and crop generic databases simulated from a 1D RT model, we trained some machine learning inversion algorithms to retrieve canopy state variables like Green Area Index GAI, Cab and Canopy Chlorophyll Content (CCC). Results on both simulations and in situ data combined with SENTINEL2 images showed that crop specific algorithms outperform the generic one for the three variables, especially when the canopy structure breaks the 1D turbid medium assumption such as in maize where rows are dominant during a significant part of the growing season
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Kenefic, Laura. "Leaf Area, Stemwood Volume Growth, and Stand Structure in a Mixed-Species, Multi-Aged Northern Conifer Forest." Fogler Library, University of Maine, 2000. http://www.library.umaine.edu/theses/pdf/KeneficLS2000.pdf.
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Deng, Yinghai 1966. "Development and disease resistance of leafy reduced stature maize (Zea mays L.)." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=38177.
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Previous studies on Leafy reduced-stature (LRS) maize found that it had extremely early maturity and a higher harvest index (HI), leading to high yields for its maturity rating. Whether this apparent high HI is relaxed to its earliness, or can also exist among the medium or late maturity LRS maize has not been previously investigated. It was also of interest to know if the traits that produced the LRS canopy structure have pleiotropic effects on root architecture. Finally, field observations indicated that LRS maize had a lower incidence of common smut. It is not known whether this apparent resistance is specific to smut or includes other diseases.Using a wide range of the most recently developed LRS hybrids and some conventional hybrids, a two-year field experiment was conducted to examine the HI and disease resistance of LRS maize. HI, yield, and yield components were compared between the two genotype groups (LRS and conventional) under different population densities. The resistance to the natural incidence of common smut and artificially inoculated Gibberella ear rot was also tested. Morphology and fractal dimension analyses of roots at an early development stage were conducted in indoor experiments. These analyses were performed with WinRHIZO (version 3.9), an interactive scanner-based image analysis system.
This work showed that: (1) There was no relationship between the HI and maturity; higher HIs can also exist among the medium and late maturity LRS hybrids. (2) While LRS maize hybrids have the potential for high yield this was not realized in the LRS hybrids used in this work. Further breeding and development of optimum management practices are needed to fully exploit this potential. (3) During early development LRS hybrids generally had more branching and more complex root systems than conventional hybrids. (4) Fractal dimension, as a comprehensive estimation of root complexity, was highly related to major root morphological variables, such as root total length, surface area, branching frequency and dry mass. (5) Of the hybrids tested the greatest resistance to both common smut and Gibberella ear rot, two major ear diseases, occurred in some of the LRS types.
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Nemchenko, Andriy. "Molecular and biochemical characterization of three lipoxygenases in maize." [College Station, Tex. : Texas A&M University, 2006. http://hdl.handle.net/1969.1/ETD-TAMU-1844.
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Guiterman, Christopher Henry. "The Influences of Conventional and Low Density Thinning on Leaf Area, Growth, and Growing Space Relationships of Eastern White Pine (Pinus Strobus L.)." Fogler Library, University of Maine, 2009. http://www.library.umaine.edu/theses/pdf/GuitermanCH2009.pdf.
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Blancon, Justin. "Identification des déterminants génétiques de la tolérance à la sècheresse chez le maïs par l'étude de l'évolution de l'indice foliaire vert au cours du cycle de la plante et le développement d'une méthode de phénotypage innovant." Thesis, Université Clermont Auvergne (2017-2020), 2019. http://www.theses.fr/2019CLFAC026.
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D’ici la fin du siècle, les prévisions climatiques prévoient une diminution de la quantité et de la régularité des pluies s’accompagnant d’une augmentation du risque de sècheresse en Europe et dans de nombreuses régions du monde. La création de nouvelles variétés de maïs plus tolérantes au stress hydrique est un levier indispensable pour faire face à ces contraintes futures. L’objectif principal de cette thèse est d’approfondir les connaissances des déterminismes génétiques de la tolérance à la sècheresse chez le maïs. Pour ce faire, il est proposé de disséquer ce caractère complexe en caractères physiologiques sous-jacents dont le déterminisme génétique est a priori plus simple. L’évolution de l’indice foliaire vert (GLAI : Green Leaf Area Index) au cours du cycle de la plante, par son rôle majeur dans l’interception lumineuse, la transpiration et les échanges de CO2, est un caractère secondaire prometteur pour identifier les bases génétiques de la tolérance à la sècheresse et en améliorer la compréhension. Au cours de cette thèse, nous avons développé une méthode de phénotypage haut débit permettant d’estimer la cinétique du GLAI au champ. Cette méthode combine la caractérisation multispectrale par drone et l’utilisation d’un modèle physiologique simple de GLAI. Elle permet d’estimer la cinétique du GLAI de manière continue sur l’ensemble du cycle de la plante avec une bonne précision, tout en divisant par vingt le temps nécessaire au phénotypage. Nous avons utilisé cette méthode lors de deux essais en conditions optimales et deux essais en conditions de stress hydrique pour mesurer l’évolution du GLAI au sein d’un panel de 324 lignées issues d’une population MAGIC (Multi-parent Advanced Generation Inter-Cross). Les cinétiques estimées présentent une forte héritabilité et expliquent une part significative du rendement en conditions optimales et stressées. Afin d’identifier les bases génétiques de la cinétique du GLAI, trois approches de génétique d’association longitudinales ont été comparées : une approche univariée en deux étapes, une approche multivariée en deux étapes et une approche de régression aléatoire en une étape. Ces trois approches, couplées à la forte densité des données de génotypage disponibles (près de 8 millions de marqueurs), ont permis de révéler de nombreux QTL (Quantitative Trait Loci), dont certains colocalisent avec des QTL de rendement. Enfin, nous avons démontré que les QTL de GLAI identifiés lors de cette étude pouvaient expliquer près de 20 % de la variabilité du rendement observée dans un large réseau d’expérimentations sous stress hydrique. Ce travail fournit des méthodes qui permettront une meilleure caractérisation et une meilleure compréhension des déterminismes génétiques de la cinétique du GLAI, un caractère jusqu’ici inaccessible pour les populations de taille importante. Ce caractère présente toutes les caractéristiques requises pour améliorer l’efficacité des programmes de sélection en conditions de stress hydriqueBy the end of the century, climate forecasts predict a decrease in the quantity and regularity of rainfall with an increasing risk of drought in Europe and in many regions of the world. Breeding for more tolerant varieties will be an essential lever to face these future constraints. The main objective of this work is to characterize the genetic determinisms of drought tolerance in maize. To this aim, it is proposed to dissect this complex trait into underlying physiological traits whose genetic determinism is supposed to be simpler. Green Leaf Area Index (GLAI) dynamics throughout the plant cycle, through its major role in light interception, transpiration and CO2 exchange, is a promising secondary trait to identify and better understand the genetic basis of drought tolerance. During this thesis, we developed a high-throughput method for phenotyping maize GLAI dynamics in the field. This method combines UAV multispectral imagery and a simple GLAI model. It makes possible the estimation of the dynamics of GLAI continuously throughout the whole plant cycle with good accuracy, while reducing the phenotyping time twentyfold. This method was used in two well-watered and two water-deficient trials to characterize the GLAI dynamics of 324 lines from a MAGIC population (Multi-parent Advanced Generation Inter-Cross). The estimated dynamics have a high heritability and explain a significant part of grain yield under well-watered and water-stressed conditions. To characterize the genetic basis of GLAI dynamics, three longitudinal GWAS (Genome Wide Association Study) approaches were compared: a univariate two-step approach, a multivariate two-step approach and a random regression one-step approach. These three approaches, combined with the high density of available genotyping data (nearly 8 million markers), have revealed many QTL (Quantitative Trait Loci), some of which were co-localized with yield QTL. Finally, we demonstrated that the GLAI QTL identified in this study could explain nearly 20 % of the grain yield variability observed in a large network of water-stressed experiments. This work provides methods that will enable a better characterization and understanding of the genetic determinisms of GLAI dynamics, a trait that was out of reach in large populations until now. This trait presents all the characteristics required to improve the effectiveness of selection programs under water stress conditions
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Pace, Micah D. "Effect of Stand Density on Behavior of Leaf Area Prediction Models for Eastern White Pine (Pinus strobus L.) in Maine." Fogler Library, University of Maine, 2003. http://www.library.umaine.edu/theses/pdf/PaceMD2003.pdf.
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Harvey, Abby (Abigail P. ). "Sources of arsenic and lead in drinking water of Eastport, Perry, and Pleasant Point, Maine." Thesis, Massachusetts Institute of Technology, 2018. http://hdl.handle.net/1721.1/119317.
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Thesis: M. Eng., Massachusetts Institute of Technology, Department of Civil and Environmental Engineering, 2018.Cataloged from PDF version of thesis.
Includes bibliographical references (pages 58-61).
Lead and arsenic in drinking water are a health risk to communities throughout the world; lead can be a problem in houses with old piping systems with either lead piping or 50/50 lead solder, and groundwater in Maine contains high arsenic concentrations. This study sought to determine the prevalence and sources of arsenic and lead in the drinking water of Eastport, Perry, and Pleasant Point, Maine. Citizens of these towns submitted water samples from their homes, and arsenic and lead were measured in these samples. Each citizen submitted two samples: one where water stood in the pipes for a minimum of six hours, and another where the tap was flushed for 2+ minutes before sample collection. The primary water sources in the region were municipal water, from the Passamaquoddy Water District (PWD), and well water from private wells. Water samples were also collected from the source waters of the municipal water system, the Passamaquoddy Water District, and immediately following water treatment to determine sources of lead in the municipal system. Lead concentrations were found to be below the Environment Protection Agency (EPA) action level of 15ppb throughout the municipal system, and less than 1% of PWD samples exceeded the action level for lead in the standing samples. Overall, including houses with wells, 2% of houses exceeded the EPA action level in standing samples, and these houses are inferred to contain high lead levels in their piping. Arsenic levels in well water samples were found to exceed the EPA Guideline of 10[mu]g/L in 15% of samples, and did not depend on bedrock type, pH, or well depth, suggesting that bedrock heterogeneity and fracture geometry plays a large role in arsenic concentrations in this region.
by Abby Harvey.
M. Eng.
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Phiri, Donald Mwelwa. "The effect of leguminous browse supplementation on maize husk utilization by goats indigenous to the eastern province of Zambia /." Thesis, McGill University, 1991. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=60531.
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Small maize livestock farmers in the Eastern Province of Zambia badly need forage high in protein to supplement the protein deficient pasture grass. Nutrient intake, especially for the small ruminants with small gastro-intestinal capacity compared to metabolizable energy requirement, will be near or below maintenance if these poor quality feeds are not supplemented. High quality supplements however, are beyond the reach of the small scale farmers. The effect of feeding maize husk and leucaena as a mixture or separately on voluntary intake of maize husk was studied over a 40 day period. Ten male goats with an average weight of 15 kg were used. In a separate experiment the effect of browse supplementation on maize husk utilization by goats was studied. The treatments were; maize husk plus urea; maize husk plus Leucaena leucocephala (3:2); maize husk plus Calliandra calothyrsus (3:2); and maize husk plus leucaena plus calliandra (3:1:1). The results of the study indicate that leucaena and calliandra are both potentially valuable feed components.
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Begna, Sultan Hussein. "Response of leafy reduced-stature maize (Zea mays L.) hybrids to plant population densities and planting patterns in a short-season area." Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=27278.
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The yield of short-season hybrids is lower than long-season hybrids, mainly as a result of the low final leaf area developed by the short-season plants. This is due to the smaller plant stature and smaller leaf number and size of short-season adapted hybrids. In addition, in short-season areas the thermal-time available may be insufficient to mature grain of current maize hybrids. Maize hybrids which accumulate leaf area quickly, mature earlier, yield well and tolerate higher population densities better than the currently available hybrids would be more suitable for production in short-season areas. The "Leafy reduced-stature" maize hybrids, which have only recently been developed, have traits which address these criteria. There has been no previous effort to evaluate the effects of more than two plant population densities or the effects of planting patterns on the yield, yield components and vegetative growth of these hybrids. in 1995, field experiments were conducted at two sites near Montreal to compare the response of leafy reduced-stature (LRS), non-leafy normal stature (NLNS), and non-leafy reduced-stature (NLRS) maize hybrids to plant population densities and planting patterns. LRS maize hybrids showed the most rapid growth of the first ear, and the highest yield per single plant and per hectare at high plant population densities in paired rows. LRS hybrids also had longer grain filling periods, lower grain moisture contents and higher harvest indices than conventional (NLNS) hybrids. Rapid growth of the first ear and a higher harvest index are indications that LRS hybrids should be more tolerant of higher population densities than currently available hybrids. Therefore, LRS hybrids show promise for production in short-season areas at high plant population densities where maize cultivation is not currently economical due to shortness of the growing-season.
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Hussein, Begna Sultan. "Response of leafy reduced-stature maize, Zea mays L., hybrids to plant population densities and planting patterns in a short-season area." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0006/MQ29652.pdf.
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