Academic literature on the topic 'Magneto-laser therapy'

Magneto-plasmonic nanocomposites can possess properties inherent to both individual components (iron oxide and gold nanoparticles) and are reported to demonstrate high potential in targeted drug delivery and therapy. Herein, we report on Fe3O4/Au magneto-plasmonic nanocomposites (MPNC) synthesized with the use of amino acid tryptophan via chemical and photochemical reduction of Au ions in the presence of nanosized magnetite. The magnetic field (MF) induced aggregation was accompanied by an increase in the absorption in the near-infrared (NIR) spectral region, which was demonstrated to provide an enhanced photothermal (PT) effect under NIR laser irradiation (at 808 nm). A possibility for therapeutic application of the MPNC was illustrated using cancer cells in vitro. Cultured HeLa cells were treated by MPNC in the presence of MF and without it, following laser irradiation and imaging using confocal laser scanning microscopy. After scanning laser irradiation of the MPNC/MF treated cells, a formation and rise of photothermally-induced microbubbles on the cell surfaces was observed, leading to a damage of the cell membrane and cell destruction. We conclude that the synthesized magneto-plasmonic Fe3O4/Au nanosystems exhibit magnetic field-induced reversible aggregation accompanied by an increase in NIR absorption, allowing for an opportunity to magnetophoretically control and locally enhance a NIR light-induced thermal effect, which holds high promise for the application in photothermal therapy.

Aim. To study clinical and epidemiological data in males and females with ureaplasma infection and to evaluate efficacy of magneto-laser therapy used as additional treatment of ureaplasma infection. Methods. 104 patients (94 men and 10 women) with urogenital ureaplasma infection were observed. Patients were divided into two groups: a study group (n=55) that received standard and magneto-laser therapy, and a comparison group (n=49) that received only standard treatment. Polymerase chain reaction was used to investigate samples for Mycoplasma hominis, Ureaplasma parvum and urealyticum, and bacteriological study for Mycoplasma hominis and Ureaplasma spp. was additionally performed with determining their antibiotic susceptibility. Magnetic therapy was conducted with the use of Michelangelo device (Italy) for 10 minutes to small pelvis area for 10 days. Results. As a result, 78 (82.9%) males were diagnosed with uretritis, 52 (55.3%) with prostatitis, 37 (39.3%) with cystitis. In females monoinfection was more prevalent than in males (50.0% vs 40.4%). Ureaplasmosis predominantly affected subjects aged 20-29 (97.8%) and 30-39 (86.0%) years. In female group, patients aged 20-29 years prevailed, while in a male group - patients aged 30-39 years. In males, the association of Ureaplasma with Mycoplasma hominis (36.1%) prevailed. Conclusion. Complex treatment of ureaplasma infection of urogenital tract including magneto-laser therapy demonstrated high clinical efficacy and allowed achieving clinical and laboratory cure of ureaplasma infection in 85.4% of cases.

In recent years there has been growing evidence to suggest a major role of basophils alongside eosinophils and mast cells in allergic inflammation. The aim of this study was to analyze the dynamics of the basophil phenotypes after the use of magneto-la-ser therapy in children with atopic bronchial asthma. Materials and methods. A total of 66 children with mild persistent atopic bronchial asthma (aged 6 to 18 years old) were examined. Group A included 34 children who received magneto-laser therapy together with basic asthma treatment (low dose of inhaled corticosteroids). Group B included 32 children who received only basic asthma therapy. The level of CD203с + , CD203с + CD63 + , CD203с + IgE + basophils was determined in peripheral blood in the beginning of the study, after 2 weeks and after 3 months. Results. A statistically significant decrease in the absolute levels of CD203с+CD63+ and CD203с + IgE + basophils and in the relative level of CD203с + IgE + among all CD203с + basophils was determined in group A after magneto-laser therapy. The comparison of group A and group B indices revealed a significant difference between the relative level of CD203с + IgE + basophils after 12-15 days from the beginning of the study. This indicator was significantly lower in group A than in group B (p<0.05). The absolute level of CD203с + IgE + basophils was significantly lower in group A in comparison with group B after 82-90 days (p<0.05). Conclusions. Magneto-laser therapy can change the phenotype of basophils in children with atopic bronchial asthma, causing suppression of proallergic pa-rameters. Considering these results there is reason to believe, that it is possible to use this method as an additional immunocorrective treatment in patients with basophilic phenotype of atopic asthma.

In recent years there has been growing evidence to suggest a major role of basophils alongside eosinophils and mast cells in allergic inflammation. The aim of this study was to analyze the dynamics of the basophil phenotypes after the use of magneto-la-ser therapy in children with atopic bronchial asthma. Materials and methods. A total of 66 children with mild persistent atopic bronchial asthma (aged 6 to 18 years old) were examined. Group A included 34 children who received magneto-laser therapy together with basic asthma treatment (low dose of inhaled corticosteroids). Group B included 32 children who received only basic asthma therapy. The level of CD203с + , CD203с + CD63 + , CD203с + IgE + basophils was determined in peripheral blood in the beginning of the study, after 2 weeks and after 3 months. Results. A statistically significant decrease in the absolute levels of CD203с+CD63+ and CD203с + IgE + basophils and in the relative level of CD203с + IgE + among all CD203с + basophils was determined in group A after magneto-laser therapy. The comparison of group A and group B indices revealed a significant difference between the relative level of CD203с + IgE + basophils after 12-15 days from the beginning of the study. This indicator was significantly lower in group A than in group B (p<0.05). The absolute level of CD203с + IgE + basophils was significantly lower in group A in comparison with group B after 82-90 days (p<0.05). Conclusions. Magneto-laser therapy can change the phenotype of basophils in children with atopic bronchial asthma, causing suppression of proallergic pa-rameters. Considering these results there is reason to believe, that it is possible to use this method as an additional immunocorrective treatment in patients with basophilic phenotype of atopic asthma.

The purpose of this study is to evaluate the outcome of regenerative therapy of peri-implantitis. The 32 patients involved in this study with inflammatorydestructive processes in the field of peri-implant tissues of osseointegrated implants. The diagnostic parameters used for assessing peri-implantitis include clinical indices, Probing Pocket Depth (PPD), Bleeding On Probing (BOP), periimplant radiography. 16 implants with peri-implant mucositis, 8 implants with early peri-implantitis and 5 implants with moderate peri-implantitis was treated only conservative treatments methods, 6 implants with early peri-implantitis, 7 implants with moderate peri-implantitis and 4 implants with severe periimplantitis was treated surgically. PPD and BOP data at the re-examination were retrospectively compared to baseline data. A statistical significant reduction in both PPD and BOP were seen at alltimepoints as compared with the baseline clinical measurements. Stable clinical measurements PPD and BOP were demonstrated after 1 year the initial treatment, remaining stable during the following three years. Surgical regenerative treatment combined with mechanical and chemical detoxification of the implants’ surface, magneto-laser therapy and regenerative therapy using an autologous bone, xenograft, hyaluronic acid and a restorablemembrane a reliable method for stopping and treatment peri-implantitis. Key words: Peri-Implantitis; Dental Implant; Regenerative Therapy; Magneto-Laser Therapy; Hyaluronic Acid.

In this study, we investigated the hyperthermia efficiency of magnetic hyperthermia therapy (MHT), photo-thermal therapy (PTT), and the combination of both techniques by employing SPIO-based magneto-nanomicelles as the heating agents. Magneto-nanomicelles in aqueous suspension were exposed to 808-nm laser irradiation (PTT mode), alternating magnetic field (MHT mode), and both modalities (DUAL mode). All the three methods can offer effective temperature increases (above 20 °C). DUAL-mode resulted in an approximately 2-fold increase in heating efficiency (36 °C) compared with PTT or MHT alone. For in vivo experiments, a total of 24 Lewis carcinoma-bearing mice were randomly divided into four groups: the control group (no therapy), PTT, MHT, and DUAL group. In the three therapy groups, magneto-nanomicelles were injected into the tumor and the corresponding treatment measures were performed every other day for a total of three times each. MRI scans were used to calculate tumor volume after each treatment. One-way analysis of variance (ANOVA) was employed to compare the curative effect of different treatment groups. Compared with the control group, PTT, MHT, and DUAL groups all showed a significant inhibitory effect on tumor volume (P < 0.05). In the DUAL group, the mean tumor volume was smaller than that of the PTT or the MHT group. Our work demonstrated that hyperthermia using SPIO-based magnetonanomicelles has a remarkable suppressive effect in anticancer therapy. Moreover, the combined model of hyperthermia in vivo can achieve synthetic effects with shorter healing time by using the same magneto-nanomicelles.

There is a consensus about the benefit of exercise in shoulder periarthritis. However, there is uncertainty about the optimal combination of physical factors, the upgrading effect of exercise over physiotherapy and about the exercise parameters, such as frequency, intensity and duration. The aim of this study is to assess the upgrading effect of exercise. A two-week effect between two combinations of physical factors without exercise was compared versus the same double combinations of physical factors with exercise. To estimate the better combination between these two double physical factors, their two-week effect was compared. To evaluate the prophylactic effect of the frequency, the intensity and the duration of the home-prescribed exercise, these parameters were analysed after six months in all patients. Forty-eight outpatients (age 56.2±15.69 years) with shoulder periarthritis (pain initiation 3.39±3.15 months before recruitment) were treated for two weeks and followed up six months later. They were divided into four treatment groups of 12 patients. The first group was treated with magnetic field and electrophoresis with lidocaine. The second group received the same combination and exercise. The third group was treated with interferential current and laser. The fourth group received the same combination and exercise. The pain was recorded before and after the daily procedures by visual-analogue scale. Before and after the two-week treatment course, shoulder range of motion and manual muscle testing were performed. After the treatment course, each patient was instructed to perform home-exercises. After six months follow-up the frequency, the intensity and the duration of the pain were recorded, as well as the frequency, the intensity and the duration of the home-exercises. ANOVA, correlation and regression analysis were applied in the statistical analysis. After the treatment course in all patients there was significant improvement regarding pain (P < 0.05), muscle strength (P < 0.05) and shoulder mobility (P < 0.05). The most significant results were recorded in the combination of interferential current, laser and exercises (P < 0.05); followed by the combination of interferential current and laser (P < 0.05); followed by the combination of electrophoresis, magneto-therapy and exercises (P < 0.05); and finally by the combination of electrophoresis and magneto-therapy (P < 0.05). There was an upgrading effect of exercise over physiotherapy (P > 0.05). There was a correlation between pain frequency and home-exercise frequency (P < 0.05). The pain frequency tended to zero at more than five times daily home-exercises (P < 0.05). Over the two-week therapeutic course the lowest pain and the highest mobility and strength showed the combination of interferential current, laser and exercises; followed by the combination of interferential current and laser; followed by the combination of electrophoresis, magneto-therapy and exercises; and finally the combination of electrophoresis and magneto-therapy. There was an upgrading effect of exercise over physiotherapy. Only the frequency of the exercise had a prophylactic effect. The incidence of pain tended to zero at exercise frequency over five times daily.

In this work, we aimed to develop liposomal nanocomposites containing citric-acid-coated iron oxide magnetic nanoparticles (CMNPs) for dual magneto-photothermal cancer therapy induced by alternating magnetic field (AMF) and near-infrared (NIR) lasers. Toward this end, CMNPs were encapsulated in cationic liposomes to form nano-sized magnetic liposomes (MLs) for simultaneous magnetic hyperthermia (MH) in the presence of AMF and photothermia (PT) induced by NIR laser exposure, which amplified the heating efficiency for dual-mode cancer cell killing and tumor therapy. Since the heating capability is directly related to the amount of entrapped CMNPs in MLs, while the liposome size is important to allow internalization by cancer cells, response surface methodology was utilized to optimize the preparation of MLs by simultaneously maximizing the encapsulation efficiency (EE) of CMNPs in MLs and minimizing the size of MLs. The experimental design was performed based on the central composite rotatable design. The accuracy of the model was verified from the validation experiments, providing a simple and effective method for fabricating the best MLs, with an EE of 87% and liposome size of 121 nm. The CMNPs and the optimized MLs were fully characterized from chemical and physical perspectives. In the presence of dual AMF and NIR laser treatment, a suspension of MLs demonstrated amplified heat generation from dual hyperthermia (MH)–photothermia (PT) in comparison with single MH or PT. In vitro cell culture experiments confirmed the efficient cellular uptake of the MLs from confocal laser scanning microscopy due to passive accumulation in human glioblastoma U87 cells originated from the cationic nature of MLs. The inducible thermal effects mediated by MLs after endocytosis also led to enhanced cytotoxicity and cumulative cell death of cancer cells in the presence of AMF–NIR lasers. This functional nanocomposite will be a potential candidate for bimodal MH–PT dual magneto-photothermal cancer therapy.

The combination of magnetic and plasmonic properties at the nanoscale promises the development of novel synergetic image-guided therapy strategies for the treatment of cancer and other diseases, but the fabrication of non-contaminated magneto-plasmonic nanocomposites suitable for biological applications is difficult within traditional chemical methods. Here, we describe a methodology based on laser ablation from Fe target in the presence of preliminarily ablated water-dispersed Au nanoparticles (NPs) to synthesize ultrapure bare (ligand-free) core-satellite nanostructures, consisting of large (several tens of nm) Fe-based core decorated by small (mean size 7.5 nm) Au NPs. The presence of the Fe-based core conditions a relatively strong magnetic response of the nanostructures (magnetization of >12.6 emu/g), while the Au NPs-based satellite shell provides a broad extinction peak centered at 550 nm with a long tale in the near-infrared to overlap with the region of relative tissue transparency (650–950 nm). We also discuss possible mechanisms responsible for the formation of the magnetic-plasmonic nanocomposites. We finally demonstrate a protocol to enhance colloidal stability of the core-satellites in biological environment by their coating with different polymers. Exempt of toxic impurities and combining strong magnetic and plasmonic responses, the formed core-satellite nanocomposites can be used in biomedical applications, including photo- and magneto-induced therapies, magnetic resonance imaging or photoacoustic imaging.

Обсяг дипломної роботи становить 54 сторінки, міститься 16 ілюстрацій, 15 таблиць. Загалом опрацьовано 41 джерел. Актуальність: результати досліджень, які будуть проведені за допомогою даного приладу зможуть покращити якість життя. Мета: проектування приладу неінвазивного впливу для комплексної фізіотерапії при лікування цукрового діабету. Завдання: визначити методи впливу фізичних факторів на стан підшлункової залози;Ознайомитися з наявними приладами;Розробити структуру приладу;Визначити параметри магнітного поля, еклектростимулюючих сигналів, параметри світлоопромінюючих приладів; Продумати конструкцію приладу комплексної стимуляції;Визначити спосіб введення біологічного зворотного зв’язку. Основні результати: за результатами літературного аналізу було зроблено висновки які свідчать про розвиток напряму магнітолазерної терапії, але на даний час не було створено прилад на блютуз підсилювачі. Отримавши певну базу знань про вже існуючі прилади, розроблено структуру приладу.
The volume of the thesis is 54 pages, contains 16 illustrations, 15 tables. In total 41 sources were processed. Relevance: the research results that will be carried out with this device will improve the quality of life. Goal: designing a non-invasive effect device for complex physiotherapy in the treatment of diabetes mellitus. Objective: To determine the methods of the influence of physical factors on the state of the pancreas; Look at the available devices; Develop the structure of the device; Determine the parameters of the magnetic field, electrostatic signals, parameters of the light emitting devices; Think about the design of a complex stimulation device; Determine the way of introducing a biological feedback. The main results: according to the results of the literary analysis, conclusions were drawn that indicate the development of the direction of magnetic laser therapy, but at the present time there was no device for bluetooth amplifiers. Having received a certain knowledge base about existing devices, the structure of the device was developed.