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1
Rogers, Stephen, Sandra Sardanelli, and Inga Zasada. "Application of alkaline-stabilised biosolids for Meloidogyne incognita suppression in microplots." Nematology 9, no. 1 (2007): 123–29. http://dx.doi.org/10.1163/156854107779969682.
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AbstractN-Viro Soil (NVS) is an alkaline-stabilised biosolid that has been shown to suppress Meloidogyne incognita. In separate microplot studies, NVS was applied either alone at different rates (0, 25, 50, 75, 100 dry t ha−1), or in combination with M. incognita-resistant and M. incognita-susceptible cultivars, to different M. incognita initial densities (0, 37 500, 75 000 eggs/microplot). NVS suppressed M. incognita. During year 1, increasing rates of NVS resulted in higher soil solution pH and greater M. incognita J2 and egg suppression. Soil solution pH remained higher in NVS-amended plots compared to the unamended control in years 2 and 3. NVS was more effective in reducing moderate than in reducing high initial nematode populations, with 94-100% reduction in egg and juvenile populations compared to 75-79%, respectively. In all experiments, a reduction in nematode populations by NVS, alone or in combination with a resistant cultivar, did not occur consistently in years 2 or 3. Unfortunately, the application rate of NVS required to achieve this reduction in nematode populations is probably not agronomically realistic. Additional research may allow the rate of NVS required to suppress plant-parasitic nematodes to be reduced.
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Waldner, Matthew, Andrea Kinnear, Elhem Yacoub, Tim McAllister, Karen Register, Changxi Li, and Murray Jelinski. "Genome-Wide Association Study of Nucleotide Variants Associated with Resistance to Nine Antimicrobials in Mycoplasma bovis." Microorganisms 10, no. 7 (July 6, 2022): 1366. http://dx.doi.org/10.3390/microorganisms10071366.
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Antimicrobial resistance (AMR) studies of Mycoplasma bovis have generally focused on specific loci versus using a genome-wide association study (GWAS) approach. A GWAS approach, using two different models, was applied to 194 Mycoplasma bovis genomes. Both a fixed effects linear model (FEM) and a linear mixed model (LMM) identified associations between nucleotide variants (NVs) and antimicrobial susceptibility testing (AST) phenotypes. The AMR phenotypes represented fluoroquinolones, tetracyclines, phenicols, and macrolides. Both models identified known and novel NVs associated (Bonferroni adjusted p < 0.05) with AMR. Fluoroquinolone resistance was associated with multiple NVs, including previously identified mutations in gyrA and parC. NVs in the 30S ribosomal protein 16S were associated with tetracycline resistance, whereas NVs in 5S rRNA, 23S rRNA, and 50S ribosomal proteins were associated with phenicol and macrolide resistance. For all antimicrobial classes, resistance was associated with NVs in genes coding for ABC transporters and other membrane proteins, tRNA-ligases, peptidases, and transposases, suggesting a NV-based multifactorial model of AMR in M. bovis. This study was the largest collection of North American M. bovis isolates used with a GWAS for the sole purpose of identifying novel and non-antimicrobial-target NVs associated with AMR.
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Stanly, Christopher, Mariaevelina Alfieri, Alfredo Ambrosone, Antonietta Leone, Immacolata Fiume, and Gabriella Pocsfalvi. "Grapefruit-Derived Micro and Nanovesicles Show Distinct Metabolome Profiles and Anticancer Activities in the A375 Human Melanoma Cell Line." Cells 9, no. 12 (December 21, 2020): 2722. http://dx.doi.org/10.3390/cells9122722.
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Fruit juice is one of the most easily accessible resources for the isolation of plant-derived vesicles. Here we found that micro- and nano-sized vesicles (MVs and NVs) from four Citrus species, C. sinensis, C. limon, C. paradisi and C. aurantium, specifically inhibit the proliferation of lung, skin and breast cancer cells, with no substantial effect on the growth of non-cancer cells. Cellular and molecular analyses demonstrate that grapefruit-derived vesicles cause cell cycle arrest at G2/M checkpoint associated with a reduced cyclins B1 and B2 expression levels and the upregulation of cell cycle inhibitor p21. Further data suggest the inhibition of Akt and ERK signalling, reduced intercellular cell adhesion molecule-1 and cathepsins expressions, and the presence of cleaved PARP-1, all associated with the observed changes at the cellular level. Gas chromatography-mass spectrometry-based metabolomics reveals distinct metabolite profiles for the juice and vesicle fractions. NVs exhibit a high relative amount of amino acids and organic acids whereas MVs and fruit juice are characterized by a high percentage of sugars and sugar derivatives. Grapefruit-derived NVs are in particular rich in alpha–hydroxy acids and leucine/isoleucine, myo-inositol and doconexent, while quininic acid was detected in MVs. Our findings reveal the metabolite signatures of grapefruit-derived vesicles and substantiate their potential use in new anticancer strategies.
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Vaughan, Kathleen, Lena Diaw, Wang Zhengyuan, Chia-Hao Liu, Marci Barr, Deepika Darbari, Kimberley A. Woodhouse, et al. "Comparative Clinical and Gene Expression Analysis of Sickle Cell Anemia and Hemoglobin SC Disease." Blood 124, no. 21 (December 6, 2014): 4059. http://dx.doi.org/10.1182/blood.v124.21.4059.4059.
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Abstract Homozygous hemoglobin (Hb) SS is the most common form of sickle cell disease (SCD), while HbSC represents a third of patients and has been less studied. Despite similarities in pathogenesis, there are significant clinical differences between genotypes. To elucidate any molecular differences, we compared gene expression profiles for HbSS and HbSC using microarrays. The Paxgene (PAX) blood RNA system was used to extract RNA from whole blood, which was then globin reduced. We also isolated peripheral blood mononuclear cell (PBMC) RNA to assess transcript patterns in cell populations lacking erythrocytes and granulocytes. To demonstrate clinical differences, we compared HbSS and HbSC subjects from the Bethesda Sickle Cell Cohort Study (ClinicalTrials.gov identifier: NCT00011648). Hematologic parameters were significantly different, with HbSS having higher leukocytes, lower hematocrit, and higher platelets, reticulocytes and fetal hemoglobin. Significant differences were also observed for transaminases, LDH, bilirubin, creatinine kinase, ESR and ferritin. On the other hand, HbSC patients had higher diastolic blood pressure (P=0.0009), higher SpO2 (P<0.0001), less frequent leg ulcers (Odds Ratio 2.90, 95% CI 1.15-7.35, P=0.008) and more prevalent tricuspid jet velocity above 2.9 m/s (Odds Ratio 5.07, 95% CI 1.75-14.71, P=0.0006). These associations led us to hypothesize that these are distinct forms of SCD whose biological differences might be identified with gene expression profiling. RNA was isolated from ten subjects each with HbSS, HbSC or normal volunteers (NVs) using whole blood, PBMCs and Ficoll purified PBMCs (devoid of platelets). Microarray profiling showed that SCD subjects, regardless of genotype, have significant enrichment in glucose metabolism, cellular respiration, and mitochondrial energy generation pathways compared to NVs. Next, we used hierarchical clustering to distinguish SCD expression profiles from NVs, but we were unable to further distinguish HbSS from HbSC samples using this method. We attribute the above differences between SCD and NV samples to the erythroid cell RNA expression in whole blood. There is significant overlap between the 100 most abundantly expressed transcripts and a published signature in purified reticulocytes. However, no significant differences were observed when comparing SCD and NV samples using PBMCs and Ficoll purified PBMCs. We only observed expression differences comparing HbSS and HbSC at the gene level for ATP1B1 (1.3 fold change, P=1.04E-4), WSB1 (1.3 FC, P=3.64E-3) and IL6ST (1.3 FC, P=1.13E-4), despite the evident hematological and clinical differences. ATP1B1 encodes a small ATPase for Na+/K+ transport and has hardly been explored in hemoglobinopathies. Furthermore, ATP1B1 was highly up-regulated in HbSS patients (against controls) but showed less change in HbSC patients. Our results provide an important first step towards elucidating the molecular pathophysiology that might explain the differences in clinical symptoms and survival between these 2 common forms of SCD. Disclosures No relevant conflicts of interest to declare.
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Sharma, Priyanka, Manoj Sharma, N. Vishnu S. Reddy, and Murali Chintham. "Functional Rejuvenation of Recurrent Laryngeal Nerve after Excision of Parathyroid Cyst: A Case Report and Systematic Review of Literature." An International Journal of Otorhinolaryngology Clinics 12, no. 2 (2020): 31–34. http://dx.doi.org/10.5005/jp-journals-10003-1351.
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ABSTRACT Vocal fold paralysis is a prevalent manifestation of various disorders inclusive of the commonest conditions, such as, cardiothoracic surgeries, head and neck malignancies, whereas parathyroid cysts and cervical osteophytes are the rarest causes of this common presentation. A comprehensive systematic review of the literature was done with keyword “parathyroid cysts causing vocal fold palsy” using MEDLINE, PubMed, additionally following PRISMA 2009 guidelines. A 59-year-old male patient presented with hoarseness of voice with right-sided neck swelling for one year. Excision of swelling adherent to the carotid sheath was done. Postoperative histopathological and biochemical analysis of aspirated fluid was consistent with the parathyroid cyst. Patient is in regular follow-up, and there are no clinical signs of recurrence and there is normal functioning of the affected vocal cord. This report adds to the growing knowledge of rare parathyroid cysts with unusual presentations, along with an understanding of pathophysiology, clinical diagnosis, and recovery options for vocal fold palsy due to lesions in the parathyroid gland. How to cite this article: Reddy NVS, Sharma M, Chintham M, et al. Functional Rejuvenation of Recurrent Laryngeal Nerve after Excision of Parathyroid Cyst: A Case Report and Systematic Review of Literature. Int J Otorhinolaryngol Clin 2020;12(2):31–34.
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McDonald, Ian M., Gavin D. Grant, Michael P. East, Thomas S. K. Gilbert, Emily M. Wilkerson, Dennis Goldfarb, Joshua Beri, et al. "Mass spectrometry–based selectivity profiling identifies a highly selective inhibitor of the kinase MELK that delays mitotic entry in cancer cells." Journal of Biological Chemistry 295, no. 8 (January 2, 2020): 2359–74. http://dx.doi.org/10.1074/jbc.ra119.011083.
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The maternal embryonic leucine zipper kinase (MELK) has been implicated in the regulation of cancer cell proliferation. RNAi-mediated MELK depletion impairs growth and causes G2/M arrest in numerous cancers, but the mechanisms underlying these effects are poorly understood. Furthermore, the MELK inhibitor OTSSP167 has recently been shown to have poor selectivity for MELK, complicating the use of this inhibitor as a tool compound to investigate MELK function. Here, using a cell-based proteomics technique called multiplexed kinase inhibitor beads/mass spectrometry (MIB/MS), we profiled the selectivity of two additional MELK inhibitors, NVS-MELK8a (8a) and HTH-01-091. Our results revealed that 8a is a highly selective MELK inhibitor, which we further used for functional studies. Resazurin and crystal violet assays indicated that 8a decreases triple-negative breast cancer cell viability, and immunoblotting revealed that impaired growth is due to perturbation of cell cycle progression rather than induction of apoptosis. Using double-thymidine synchronization and immunoblotting, we observed that MELK inhibition delays mitotic entry, which was associated with delayed activation of Aurora A, Aurora B, and cyclin-dependent kinase 1 (CDK1). Following this delay, cells entered and completed mitosis. Using live-cell microscopy of cells harboring fluorescent proliferating cell nuclear antigen, we confirmed that 8a significantly and dose-dependently lengthens G2 phase. Collectively, our results provide a rationale for using 8a as a tool compound for functional studies of MELK and indicate that MELK inhibition delays mitotic entry, likely via transient G2/M checkpoint activation.
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Vellios, Nikos, and Panos Tsakiropoulos. "The Effect of Fe Addition in the RM(Nb)IC Alloy Nb–30Ti–10Si–2Al–5Cr–3Fe–5Sn–2Hf (at.%) on Its Microstructure, Complex Concentrated and High Entropy Phases, Pest Oxidation, Strength and Contamination with Oxygen, and a Comparison with Other RM(Nb)ICs, Refractory Complex Concentrated Alloys (RCCAs) and Refractory High Entropy Alloys (RHEAs)." Materials 15, no. 17 (August 23, 2022): 5815. http://dx.doi.org/10.3390/ma15175815.
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In this work, the RM(Nb)IC alloy Nb–30Ti–10Si–5Cr–5Sn–3Fe–2Al–2Hf (NV2) was studied in the as-cast and heat-treated conditions; its isothermal oxidation at 700, 800 and 900 °C and its room temperature hardness and specific strength were compared with other Sn-containing RM(Nb)ICs—in particular, the alloy Nb–24Ti–18Si–5Cr–5Fe–5Sn (NV5)—and with RCCAs and RHEAs. The addition of Fe (a) stabilised Nbss; A15–Nb3X (X = Al, Si and Sn) and Nb3Si; metastable Nb3Si-m’ and Nb5Si3 silicides; (b) supported the formation of eutectic Nbss + Nb5Si3; (c) suppressed pest oxidation at all three temperatures and (d) stabilised a Cr- and Fe-rich phase instead of a C14–Nb(Cr,Fe)2 Laves phase. Complex concentrated (or compositionally complex) and/or high entropy phases co-existed with “conventional” phases in all conditions and after oxidation at 800 °C. In NV2, the macrosegregation of Si decreased but liquation occurred at T >1200 °C. A solid solution free of Si and rich in Cr and Ti was stable after the heat treatments. The relationships between solutes in the various phases, between solutes and alloy parameters and between alloy hardness or specific strength and the alloy parameters were established (parameters δ, Δχ and VEC). The oxidation of NV2 at 700 °C was better than the other Sn-containing RM(Nb)ICs with/without Fe addition, even better than RM(Nb)IC alloys with lower vol.% Nbss. At 800 °C, the mass change of NV2 was slightly higher than that of NV5, and at 900 °C, both alloys showed scale spallation. At 800 °C, both alloys formed a more or less continuous layer of A15–Nb3X below the oxide scale, but in NV5, this compound was Sn-rich and severely oxidised. At 800 °C, in the diffusion zone (DZ) and the bulk of NV2, Nbss was more severely contaminated with oxygen than Nb5Si3, and the contamination of A15–Nb3X was in-between these phases. The contamination of all three phases was more severe in the DZ. The contamination of all three phases in the bulk of NV5 was more severe compared with NV2. The specific strength of NV2 was comparable with that of RCCAs and RHEAs, and its oxidation at all three temperatures was significantly better than RHEAs and RCCAs.
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Donga, R., and J. P. Lund. "Discharge patterns of trigeminal commissural last-order interneurons during fictive mastication in the rabbit." Journal of Neurophysiology 66, no. 5 (November 1, 1991): 1564–78. http://dx.doi.org/10.1152/jn.1991.66.5.1564.
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1. The aim of these experiments was to examine the physiological properties and patterns of firing of trigeminal interneurons during fictive mastication in anesthetized and paralyzed rabbits. Antidromic stimulation was used to show that the 82 interneurons projected to the area of the contralateral fifth nerve motor nucleus (NVmot). 2. Straight-line conduction velocities calculated from stereotaxic coordinates of the stimulating and recording electrodes for 63 interneurons were found to range between 3.7 and 16.3 m/s (mean, 9.5 m/s). 3. Histological reconstructions of recording electrode tracks showed that the interneurons observed in this study were located in the lateral brain stem in or just medial to the rostral trigeminal sensory nuclei, including the intertrigeminal (NVint) and supratrigeminal (NVs) areas, the main sensory nucleus of the fifth nerve (NVsnpr), the rostral subdivision of the oral nucleus of the spinal trigeminal tract (NVor tau), and the rostral part of the parvocellular reticular nucleus (NRpc alpha). 4. Forty-six interneurons were shown to have low-threshold (LT) peripheral receptive fields, and 41 of these (88%) were in the oral cavity. Most of the responses were rapidly adapting. 5. Twenty-eight interneurons changed their pattern of firing during cortically induced fictive mastication. The discharge frequency of 20 neurons varied in phase with the fictive masticatory motor output, which was recorded from central ends of cut hypoglossal nerves (XII) and/or from the NVmot. Others were briefly excited and then inhibited (n = 2), only inhibited (n = 4), or tonically excited during fictive mastication (n = 2). Fifteen others were unaffected by this test. 6. It was found that the rhythmically active neurons could be further subdivided into two categories: those receiving short-latency excitatory input from the masticatory area of the cortex (n = 11) and those that did not (n = 9). No obvious differences in peripheral receptive fields for neurons in these categories were found. 7. We suggest that these phasically active premotor neurons are part of the circuitry generating the rhythmic masticatory pattern, specifically those that directly control the bursts of firing of the trigeminal motoneurons (burst generators, BGs). Their properties allow them to integrate sensory information and descending commands with the masticatory rhythm that is probably generated in midline brainstem reticular nuclei.
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Liu, Gang, Leying Chen, and Shimin Chen. "Zen." Proceedings of the VLDB Endowment 14, no. 5 (January 2021): 835–48. http://dx.doi.org/10.14778/3446095.3446105.
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Emerging <u>N</u>on-<u>V</u>olatile <u>M</u>emory (NVM) technologies like 3DX-point promise significant performance potential for OLTP databases. However, transactional databases need to be redesigned because the key assumptions that non-volatile storage is orders of magnitude slower than DRAM and only supports blocked-oriented access have changed. NVMs are byte-addressable and almost as fast as DRAM. The capacity of NVM is much (4-16x) larger than DRAM. Such NVM characteristics make it possible to build OLTP database entirely in NVM main memory. This paper studies the structure of OLTP engines with hybrid NVM and DRAM memory. We observe three challenges to design an OLTP engine for NVM: tuple metadata modifications, NVM write redundancy, and NVM space management. We propose Zen, a high-throughput log-free OLTP engine for NVM. Zen addresses the three design challenges with three novel techniques: metadata enhanced tuple cache, log-free persistent transactions, and light-weight NVM space management. Experimental results on a real machine equipped with Intel Optane DC Persistent Memory show that Zen achieves up to 10.1x improvement compared with existing solutions to run an OLTP database as large as the size of NVM while achieving fast failure recovery.
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Pacini, G., M. Pendolino, C. Pizzorni, E. Gotelli, A. Sulli, L. Carmisciano, S. Paolino, V. Smith, and M. Cutolo. "POS0850 NAILFOLD CAPILLARY DILATIONS IN RAYNAUD’S PHENOMENON: QUANTIFYING A PREDICTIVE THRESHOLD FOR THE ‘SCLERODERMA PATTERN’." Annals of the Rheumatic Diseases 80, Suppl 1 (May 19, 2021): 679. http://dx.doi.org/10.1136/annrheumdis-2021-eular.2014.
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Background:Non-specific abnormalities could be detected by nailfold videocapillaroscopy (NVC) in subject with primary Raynaud’s Phenomenon (RP) several years before the clinical onset of connective tissue diseases (CTD)s [1]. Previous findings from our group proved that ≤30 μm capillary dilations in RP patients have a negative predictive value for developing the ‘scleroderma pattern’ during follow-up [2].Objectives:To investigate the role of NVC >30 μm capillary dilations as positive predictive factors of the ‘scleroderma pattern’ in RP patients later developing systemic sclerosis (SSc)-related RP.Methods:A 10-year retrospective NVC-based investigation evaluated the dataset of sequential NVCs of 18 RP patients later developing SSc (cases) and 19 sex- and age-matched RP patients later developing other CTDs (controls). Both cases and controls had ≥1 NVC performed before the ‘scleroderma pattern’/CTD diagnosis (basal NVC) showing >30 μm dilated capillaries. Each NVC was qualitatively and semi-quantitatively assessed, recording number of total capillaries, number and average/site-specific diameters (arterial, apical, venous) of >30 μm dilated capillaries [3]. Statistical analysis was performed to stratify the risk of developing the ‘scleroderma pattern’.Results:Significant differences of capillary diameters were observed between cases and controls both at basal NVC and during follow-up (p<0.001). The proportion of >30 μm dilated capillaries in basal NVC was the strongest predictor of ‘scleroderma pattern’ in a median 3-year time, with a 27% cut-off (PPV 0.79, 95%CI 0.54,0.94; p<0.001). Additional “Higher risk” NVC hallmarks for ‘scleroderma pattern’ development were apical diameter >40 μm (p<0.001), venous diameter >25 µm (p<0.05) and average diameter ≥35 µm (p<0.005). Conversely, CTDs patients showed a stable NVC ‘non-scleroderma pattern’ over a median 10-year time.Conclusion:This is the first study to show that NVC-detected homogeneous and progressive capillary loop dilations in RP patients significantly contribute to predict the ‘scleroderma pattern’ evolution within a median 3-year time, possibly providing a “very early” window of opportunity in SSc pre-clinical stages.References:[1]Cutolo M et al. Expert Rev Clin Immunol. 2019;15(7):753–64. [2] Trombetta AC et al. J Rheumatol 2016;43:599–606. [3] Smith et al. Autoimmun Rev 2020; 19(3):102458.Disclosure of Interests:None declared
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Žukaitė-Zelenkienė, Gintarė, and Samanta Fominova. "Viešojo sektoriaus taikomi ribojimai NVO: Rusijos atvejis." Public Policy and Administration 21, no. 3 (September 30, 2022): 213–25. http://dx.doi.org/10.5755/j01.ppaa.21.3.30159.
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Nevyriausybinės organizacijos yra demokratinės visuomenės išraiška. Nepaisant to kartais viešasis sektorius įžvelgia jose konkurenciją savo atliekamoms funkcijoms bei grėsmę valstybės saugumui, todėl įveda veiklos ribojimus. Dažniausiai šis reiškinys išryškėja į autoritarizmą linkusiose valstybėse. Pastaraisiais metais Rusijos Federacijoje yra stebimas nevyriausybinių organizacijų (NVO) kontrolės stiprėjimas, ypač jis akivaizdus NVO, kurios dirba politikos sferoje ir/ar yra finansuojamos užsienio šaltinių lėšomis. Šiame straipsnyje analizuojami viešojo sektoriaus ribojimai NVO veiklai Rusijoje. Tyrimo rezultatai atskleidė, kad NVO veikla yra ribojama keletu aspektų: teisės burtis į asociacijas, žodžio ir susirinkimų laisvės bei finansinių ribojimų. 2012 m. ir 2015 m. priimtos įstatymų pataisos, kurios įvedė „užsienio agento“ ir „nepageidaujamų organizacijų“ sąvokas, yra labiausiai NVO veiklą Rusijoje ribojantys teisiniai veiksniai. Tyrimas atliktas remiantis dokumentų analizės bei pusiau struktūruoto interviu metodu su Rusijos NVO atstovais.
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Pendolino, M., C. Pizzorni, S. Paolino, F. Goegan, E. Gotelli, C. Schenone, F. Cattelan, et al. "FRI0257 CAPILLAROSCOPIC VERY EARLY MORPHOLOGICAL AND QUANTITATIVE SPECIFIC ABNORMALITIES ANTICIPATE THE DEVELOPMENT OF THE “SCLERODERMA PATTERN” IN PATIENTS WITH RAYNAUD’S PHENOMENON." Annals of the Rheumatic Diseases 79, Suppl 1 (June 2020): 712.2–713. http://dx.doi.org/10.1136/annrheumdis-2020-eular.4956.
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Background:Nailfold videocapillaroscopy (NVC) abnormalities in subjects with isolated Raynaud’s phenomenon (RP) may be present before transition to secondary RP(SRP) and development of a NVC “scleroderma pattern” and are known to predict for evolution to a connective tissue disease (CTD) within few years [1]. In a previous study, we have demonstrated that the very early increase of capillary diameter over 30 μm is an independent predictor for development of Systemic Sclerosis (SSc) associated SRP [2].Objectives:Present pilot retrospective study aimed to investigate in a cohort of patients affected by CTD–related RP the presence of very early capillaroscopic morphological and quantitative abnormalities in the acquired pictures of NVC performed before the development of the NVC scleroderma-pattern. In particular, the study was addressed to identify a “very early”scleroderma pattern, in order to intercept patients with RP at high risk of evolution in a CTD, specifically SSc.Methods:We selected the NVCs of 273 SSc patients presenting one of the validated NVC “scleroderma patterns”. We enrolled 26 SSc patients having a NVC analysis performed before the development ofthe “very early”NVC pattern. As controls, we evaluated 26 patients affected by other CTDs with stable non-scleroderma pattern over time. The 16 images per patient obtained by NVC examination were analyzed for total number of capillaries, number and the limbs diameters of capillaries with a diameter >30 μm, and microhemorrhages. Statistical analysis was performed using non-parametric tests.Results:All 26 SSc patients showed dilated capillaries with a diameter >30 μm in their previous NVC. Patients later developing scleroderma pattern had statically higher number and percentage of capillaries with a diameter >30 μm (p=0.0004 and p=0.0005), as well as a larger apical dilatation >40 μm (p=0.002). A progressive and significant increase in all capillary diameters were only detected in patients later diagnosed for SSc (apical p=0.006, venous p=0.02, arterial p=0.03). A significant homogeneous and progressive dilation was observed from the apical region and then involving both venous and arterial branches, only in SSc patients (p=0.002).Conclusion:Present pilot study demonstrates, for the first time that, before to develop a validated NVC scleroderma-pattern, all potential SSc patients present significant very early morphological and quantitative NVC changes. In particular, the progressive and homogeneous capillary loop dilation over 40 μm in over 40% of total number capillaries significantly could contribute to identify RP patients who will develop a SSc pattern after 4-5 years.References:[1]Cutolo M, Sulli A, Pizzorni C, Accardo S. Nailfold videocapillaroscopy assessment of microvascular damage in systemic sclerosis. J Rheumatol. 2000;27:155-60.[2]Trombetta AC, et al. J Rheumatol 2016;43:599-606.Disclosure of Interests:Monica Pendolino: None declared, Carmen Pizzorni: None declared, Sabrina Paolino: None declared, Federica Goegan: None declared, Emanuele Gotelli: None declared, Carlotta Schenone: None declared, Francesco Cattelan: None declared, Massimo Patanè: None declared, Elisa Alessandri: None declared, Alberto Sulli Grant/research support from: Laboratori Baldacci, Vanessa Smith Grant/research support from: The affiliated company received grants from Research Foundation - Flanders (FWO), Belgian Fund for Scientific Research in Rheumatic diseases (FWRO), Boehringer Ingelheim Pharma GmbH & Co and Janssen-Cilag NV, Consultant of: Boehringer-Ingelheim Pharma GmbH & Co, Speakers bureau: Actelion Pharmaceuticals Ltd, Boehringer-Ingelheim Pharma GmbH & Co and UCB Biopharma Sprl, Maurizio Cutolo Grant/research support from: Bristol-Myers Squibb, Actelion, Celgene, Consultant of: Bristol-Myers Squibb, Speakers bureau: Sigma-Alpha
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Rajendiran, K., S. Vidya, and D. Arulmozhi. "In Vitro Callus Proliferation from Leaf Explants of Green Gram after In Situ UV-B Exposure." International Journal of Applied Sciences and Biotechnology 4, no. 1 (March 31, 2016): 82–91. http://dx.doi.org/10.3126/ijasbt.v4i1.14719.
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Callus induction was tried with leaf explants (third leaf from top of canopy) harvested from in situ control and supplementary UV-B irradiated (UV-B = 2 hours daily @ 12.2 kJ m-2 d-1; ambient = 10 kJ m-2 d-1) three varieties of green gram viz. CO-8, NVL-585 and VAMBAN-2 to study their viability for germplam conservation. Callus induction occurred both in control and UV-B stressed NVL-585 leaf explants. VAMBAN-2 both in unstressed and UV-B stressed conditions did not initiate callus. Only control leaf explants from CO-8 proliferated callus. Callus of UV-B irradiated NVL-585 weighed less (51.28 %) than control. Parenchyma cells were smaller in callus inducted from in situ UV-B exposed NVL-585 leaf explants. The leaf explants from UV-B stressed NVL-585 varieties of green gram responded to in vitro callus proliferation making them fit for germplasm conservation for cultivating in UV-B elevated environment.Int J ApplSci Biotechnol, Vol 4(1): 82-91
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Mishra, Shova, Koon-Hui Wang, Brent S. Sipes, and Miaoying Tian. "Suppression of Root-Knot Nematode by Vermicompost Tea Prepared From Different Curing Ages of Vermicompost." Plant Disease 101, no. 5 (May 2017): 734–37. http://dx.doi.org/10.1094/pdis-07-16-1068-re.
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Suppression of root-knot nematodes (Meloidogyne spp.) by vermicompost tea (VCT) has been inconsistent. Greenhouse and laboratory trials were conducted to compare the effects of VCT prepared from different curing ages of vermicompost (VC) on root penetration, reproduction, and hatching of M. incognita. In the penetration experiment, zucchini (Cucurbita pepo) seedlings were drenched with VCT prepared from (i) uncured (UVC), (ii) partially cured (PVC), (iii) completely cured (CVC) vermicompost, and (iv) water or no vermicompost (NVC) 3 days prior to M. incognita inoculation. The experiment was repeated twice on cucumber (Cucumis sativus) and terminated one week after nematode inoculation. All three trials showed that UVC and PVC reduced (P ≤ 0.05) penetration of M. incognita compared with CVC and NVC. Two greenhouse trials showed that VCT from different curing ages of VC did not reduce the abundance of M. incognita juveniles in soil and eggs in roots 2.5 months after nematode inoculation. Two laboratory trials to examine hatching consistently showed that VCT from UVC and PVC suppressed hatching (P ≤ 0.05) compared with NVC, achieving 83.1% hatch reduction by UVC. Overall, VCT from UVC and PVC suppressed root penetration and hatching, but not the reproduction of M. incognita over time.
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Mokini, Zhirajr, Valentina Genocchio, Patrice Forget, and Flavia Petrini. "Metoclopramide and Propofol to Prevent Nausea and Vomiting during Cesarean Section under Spinal Anesthesia: A Randomized, Placebo-Controlled, Double-Blind Trial." Journal of Clinical Medicine 11, no. 1 (December 26, 2021): 110. http://dx.doi.org/10.3390/jcm11010110.
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Background: Intra-operative nausea, vomiting and retching (NVR) are frequently associated with subarachnoid anesthesia (SA) in women undergoing cesarean section (CS). In this study performed in women undergoing CS under SA with a risk factor control strategy, we compared saline (placebo), propofol, metoclopramide and both drugs to prevent NVR. Methods: We recorded NVR events in 110 women undergoing CS who were randomized after umbilical cord clamping to receive saline (S; n = 27), metoclopramide 10 mg (M; n = 28), propofol 1 mg/kg/h (P; n = 27) or both drugs (PM; n = 28). Results: The proportion of women with intra-operative nausea was: S: 17/27 (63%); P: 15/27 (56%); M: 13/28 (46%); PM: 6/28 (21%) (p = 0.012, Cramér’s V = 0.31 (large effect). The proportion of women with intra-operative vomiting/retching was: S: 9/27 (33%); M: 7/27 (25%); P: 3/28 (11%); PM 2/28 (7%) (p = 0.049, Cramér’s V = 0.26 (medium effect). Post-hoc multiple comparisons revealed a significant reduction in NVR episodes and NRS scores between the PM group and control. Sedation scores did not differ among groups. Conclusion: In women undergoing CS under SA with a risk factor control strategy, combined propofol and metoclopramide reduce nausea and vomiting.
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Lee, Jun, Jang-Yeon Hwang, and Jaekook Kim. "Electrode Design for High-Power Sodium-Ion Batteries: Cr Doping into Nasicon-Structured Na3V2(PO4)3 Cathode with Self-Carbon-Coating." ECS Meeting Abstracts MA2022-01, no. 4 (July 7, 2022): 539. http://dx.doi.org/10.1149/ma2022-014539mtgabs.
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NASICON-structured Na3V2(PO4)3 (NVP) is one of the most promising cathode material for rechargeable sodium-ion batteries. NVP is characterized by a robust 3D structural framework and a high operating potential; these properties have enabled it to be widely studied as a stable and high-energy density cathode material for sodium-ion batteries (SIBs).1-4 In the present study, we designed a Na3V1.6Cr0.4(PO4)3/C (NVCrP@C) cathode by implanting Cr into the crystal structure of NVP and simultaneously coating the surface of NVP with carbon for realizing high power density SIBs. NVP is fabricated using a low-cost pyro synthesis technique with the advantage of self-carbon-coating and nano sized particles are gained through this facile technique. The substitution of Cr with vanadium in the NVP structure significantly enhanced the structural stability of the electrode while the uniform and thin carbon layer improved the electrical conductivity. Interestingly, the NVCrP@C cathode showed high electrochemical activities with multiple V3+/4+/5+ redox reactions triggered by Cr3+ substitution in a wide voltage range (2.5–4.1 V). Consequently, the NVCrP@C cathode delivered excellent cycling stability over 500 cycles even at 15 C-rate and power capability up to 70 C-rate. References Y. Cao, L. Xiao, W. Wang, D. Choi, Z. Nie, J. Yu, L. V. Saraf, Z. Yang and J. Liu, Mater., 2011, 23, 3155-3160. M. Giot, L. C. Chapon, J. Androulakis, M. A. Green, P. G. Radaelli and A. Lappas, Rev. Lett., 2007, 99, 247211 M. H. Han, E. Gonzalo, G. Singh and T. Rojo, Energy Environ. Sci., 2015, 8, 81-102. H. Liu, J. Xu, C. Ma and Y. S. Meng, Chem. Comm., 2015, 51, 4693-4696
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Latalska, Małgorzata, Joanna Bartosińska, Ewa Kosior-Jarecka, Dorota Krasowska, and Jerzy Mackiewicz. "Nailfold Videocapillaroscopy in Patients with Central Serous Chorioretinopathy and Its Relationship to Morphological and Functional Findings." Journal of Clinical Medicine 9, no. 12 (November 30, 2020): 3891. http://dx.doi.org/10.3390/jcm9123891.
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The aim of the study was to evaluate the results of nailfold videocapillaroscopy (NVC) in patients with central serous chorioretinopathy (CSC) and their correlation with different functional, morphological features and general risk factors. Material and methods: The examined group included 59 CSC patients (14 F, 45 M, mean age 47.2 ± 9.4) and 53 healthy controls (13 F, 40 M, mean age 46 ± 11.5). The NVC was qualified as a normal or abnormal pattern. In the CSC group, the ophthalmoscopy, angio-Optical Coherence Tomography (OCT-A), OCT and microperimetry were performed. The medical history regarding CSC, chronic general disorders and known risk factors was recorded. Results: In the CSC group an abnormal NVC pattern was more common (33.88% CSC vs. 7.54%, p < 0.001). Dilated apical part of capillaries, microaneurysmal dilatation, megacapillaries, fresh microhaemorrhages, bizarre and broken capillaries were observed only in CSC patients. Dilation of capillaries (47.56% vs. 13.21%, p = 0.004), ramified capillaries and neoangiogenesis (38.98% vs. 5.66%, p < 0.001), glomerular loops (32.20% vs. 1.88% p < 0.001) were more frequent in the CSC group. Conclusions: The presence of various NVC abnormalities confirms microvascular involvement in CSC pathogenesis. The results correlate with visual acuity, microperimetry, OCT data and stress. The NVC technique may play a useful diagnostic and prognostic role in CSC.
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Martins, Madlles Queiroz, Fábio Luiz Partelli, Adelmo Golynski, Adésio Ferreira, Weverton Pereira Rorigues, and José Cochicho Ramalho. "Vegetative growth of 28 genotypes of Coffea canephora at 850 meters of altitude." OCTOBER 2020, no. 14(10):2020 (October 20, 2020): 1616–22. http://dx.doi.org/10.21475/ajcs.20.14.10.p2392.
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Coffea canephora plants grown at low or elevated temperatures undergo changes in photosynthetic processes, which have a direct impact on vegetative growth. This species shows plants with distinct stress tolerance mechanisms and a great genetic diversity; however, there are no studies concerning the study of altitude on plant growth for these genotypes. The objective of this study was to evaluate the growth rates of orthotropic and plagiotropic branches from 28 Coffea canephora genotypes, ca. 3 years of age, cultivated at 850 m of altitude. The experimental design was a randomized complete block design with four repetitions. Each repetition was compounded by five plants. The period of the experimental evaluation was from 01/23/2013 to 07/07/2013. We selected and identified the orthotropic and plagiotropic branches, which were evaluated bimonthly according to their length (mm), assessing the growth rates. The results were associated with climatic factors that occurred during the experimental period. The genetic material of Coffea canephora evaluated in the study shows genetic diversity to the characteristics growth rate of orthotropic and plagiotropic branches, thus, it is possible to select genotypes with potential for cultivation at 850 m of altitude. In general, genotypes of Coffea canephora exhibited variations in growth rates, mainly in response to changes in temperature, with a sharp and constant drop in growth rates between the period from 05/05/2013 to 05/ 23/2013, when the recorded temperatures were always below 13 °C. Genotypes A1, NV2 and NV1 can be pointed as the ones which showed the best vegetative growth results at 850 meters of altitude.
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Pereira, João Kleber Novais, João Agostinho Machado-Neto, Matheus Rodrigues Lopes, Fabiola Traina, Fernando Ferreira Costa, Sara Teresinha Olalla Saad, and Patricia Favaro. "Cellular and Molecular Effects of NVP-BKM120 in Lymphoblastic Leukemia Cells." Blood 124, no. 21 (December 6, 2014): 2241. http://dx.doi.org/10.1182/blood.v124.21.2241.2241.
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Abstract Background: PI3K/AKT/mTOR signaling controls most hallmarks of cancer. Constitutive activation of PIK3 pathway in T cells acute lymphoblastic leukemia (T-ALL) has been reported; in a mouse model, PI3K activation, together with MYC, cooperates in Burkitt lymphoma (BL) pathogenesis. NVP-BKM120 is an orally bioavailable 2,6-dimorpholine pyrimidine derivative, and considered a highly selective pan-class I PI3K inhibitor. In preclinical studies, it has shown efficacy in a variety of malignancies and is currently being investigated in phase I/II/III clinical testing, mainly for advanced solid tumors (clinicaltrials.gov). Aims: Here, we described the effects of the pan-PI3K/AKT/mTOR inhibitor NVP-BKM120 on T-ALL and BL cell lines. Methods: T-ALLcell lines, Jurkat and MOLT-4, and BL cell lines, NAMALWA and Daudi, were obtained from ATCC. NVP-BKM120 was kindly provided by Novartis, and was prepared as a 10mM stock solution in DMSO. Different concentrations of the drug were used as indicated, where cells treated only with DMSO served as control. Cell viability was measured by MTT. Colony formation was carried out in semisolid methyl cellulose medium. The induction of apoptosis was assessed by annexin-V-APC/PI and by caspase cleavage. The cell cycle was analyzed by a PI-staining method. Western blot analysis was performed by standard methods. Vital staining and flow cytometry analysis with acridine orange was performed for the detection and quantification of acidic vesicular organelles (AVOs). Comparisons between the two groups were performed by the t test. Pvalue <0.05 was considered statistically significant. Results: Cell viability decreased in a concentration-dependent manner, with an IC50 range of 7-8 μM and the clonogenic growth was significantly decreased at the concentration of 1μM and at 10µM the colony formation was completely inhibited in all cells tested. After 6 hours of NVP-BKM120 treatment, we observed an increase in apoptotic cells, as well as an increase in the cleavage of procaspase 3, 8 and 9 in Jurkat, MOLT-4 and NAMALWA cells. Compared with DMSO control, NVP-BKM120 does not have any effect during apoptosis induction in the Daudi cell line. NVP-BKM120 treatment also resulted in G2/M arrest, associated with a decrease in the G1population and a decrease in Cyclin B1 protein levels. Immunoblotting analysis of cells treated with the drug revealed decreased phosphorylation, in a dose-dependent manner, of AKT, P70SK6 and 4EBP1, with stable total proteins levels. Additionally, we observed a dose-dependent decrease in BAD phosphorylation, followed by an increase in BAX:BCL2 ratio. Quantification of AVOs showed a dose-dependent increase of AVOs in all cells tested, after NVP-BKM120 treatment. Conclusions: NVP-BKM120 induced apoptosis in a dose-dependent manner in Jurkat, MOLT-4 and NAMALWA cells, while effects of the drug in the Daudi cell line were mainly cytostatic. In those cells, the induction of apoptosis suggested that the death receptor and mitochondrial pathways were activated after drug treatment. In our study, we found that NVP-BKM120 decreased the phosphorylation levels of BAD, which is linked to a pro-apoptotic activity, and up-regulated the BAX:BCL2 ratio. These results are consistent with the activation of caspase-9 and 3, related to the mitochondrial apoptosis. The accumulation of leukemia cells in the G2/M phase of the cell cycle has been associated with enhanced apoptosis. Our results suggest that decreased Cyclin B1 protein expression might be the molecular mechanism through which NVP-BKM120 induces G2/M arrest. The effects of NVP-BKM120 on the PI3K pathway indicate that NVP-BKM120 treatment may overcome rapamycin-induced AKT activation. P70SK6 and 4EBP1 are the two best-characterized substrates of mTOR1. Hence, the decrease in the phosphorylation levels of P70SK6 and 4EBP1 results in impaired oncogenic protein synthesis. Moreover, P70SK6 is one of the kinases whose phosphorylation by mTOR1 results in opposing autophagy. Accordingly, NVP-BKM120 resulted in increased AVOs, which is a characteristic feature of cells engaged in autophagy. In summary, our present study establishes that NVP-BKM120 effectively presents an antitumor activity against T-ALL and BL cell lines. The reduction of proliferation is possibly by down-regulation of Cyclin B1 and the increased BAX:BCL2 ratio is one of the mechanisms involved in the induction of apoptosis. Disclosures Off Label Use: NVP-BKM120 is an orally bioavailable 2,6-dimorpholino pyrimidine derivative, and considered a highly selective pan-class I PI3K inhibitor. .
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P.S. KHAPTE, H.M. MEENA, PRADEEP KUMAR, UDAY BURMAN, ANURAG SAXENA, and PRAVEEN KUMAR. "Influence of different protected cultivation structures on performance of cucumber (Cucumis sativus L.) in Indian hot arid region." Journal of Agrometeorology 23, no. 3 (October 5, 2021): 265–71. http://dx.doi.org/10.54386/jam.v23i3.25.
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The performance of gynoecious cucumber (cv. Terminator) was evaluated under three protected structures viz., naturally ventilated polyhouse (NVP), insect proof net house (INH) and shade net house (SNH) at Jodhpur, Rajasthan in hot arid region of India. The photosynthetically active radiation (PAR) inside these structures during cropping period ranged from 154-842 μmol m-2 s-1 which was much lower than the outside. Among structures, air temperature was 1.2°C and 0.7°C lower while relative humidity (RH) was 17 and 4 per cent higher in NVP and SNH respectively as compared to INH. Relatively low air as well as soil temperature, coupled with high RH and optimal radiation in NVP led to better plant growth and physiological activity which resulted in 42 and 142per cent higher yield than INH and SNH, respectively. Hence, it can be recommended that NVP is the best low-tech protected structure which modifies the microclimate favouring successful cultivation of greenhouse cucumber in Indian hot arid regions.
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Wiedmann, M., T. Bluethner, M. Niederhagen, K. Schoppmeyer, J. Moessner, and K. Caca. "Two novel histone deacetylase inhibitors NVP-LAQ824 and NVP-LBH589 are active against biliary tract cancer and potentiate the efficacy of gemcitabine." Journal of Clinical Oncology 24, no. 18_suppl (June 20, 2006): 4149. http://dx.doi.org/10.1200/jco.2006.24.18_suppl.4149.
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4149 Background: Chromatin remodelling agents such as histone deacetylase inhibitors have been shown to modulate gene expression in tumor cells and inhibit tumor growth and angiogenesis. NVP-LAQ824 and NVP-LBH589 are two novel chemical entities belonging to a cinnamic hydroxamic acid class of compounds. Little is known about their efficacy for the treatment of biliary tract cancer. Methods: Cell-growth inhibition by NVP-LAQ824 and NVP-LBH589 was studied in vitro in 7 human biliary tract cancer cell lines by MTT assay. In addition, the anti-tumoral effect of NVP-LBH589 was studied in a chimeric mouse model. Anti-tumoral drug mechanism was assessed by immunoblotting for p21WAF-1, acH3Lys9 and acH4, cell cycle analysis, PARP assay, TUNEL assay, and immunhistochemistry for MIB-1. Results: In vitro treatment with both compounds significantly suppressed the growth of all cancer cell lines (mean IC50 (3d) 0.08 and 0.04 μM, respectively) and was associated with hyperacetylation of nucleosomal histones H3 and H4, increased expression of p21WAF-1, cell cycle arrest at G2/M-checkpoint, and induction of apoptosis (PARP cleavage). After 28 d, NVP-LBH589 reduced tumor mass by 66% (bile duct cancer) and 87% (gallbladder cancer) in vivo in comparison to placebo and potentiated the efficacy of gemcitabine. Further analysis of the tumor specimens revealed increased apoptosis (TUNEL) and reduced cell proliferation (MIB-1). Conclusions: Our findings suggest that NVP-LBH589 > NVP-LAQ824 are active against human biliary tract cancer in vitro. In addition, NVP-LBH589 demonstrated significant in vivo activity and potentiated the efficacy of gemcitabine. Therefore, further clinical evaluation of this new drug for the treatment of biliary tract cancer is recommended. No significant financial relationships to disclose.
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Lee, Yongseok, Jung-Keun Yoo, Youngseok Oh, Hyunyoung Park, Wonseok Go, Seung-Taek Myung, and Jongsoon Kim. "Unexpectedly high electrochemical performances of a monoclinic Na2.4V2(PO4)3/conductive polymer composite for Na-ion batteries." Journal of Materials Chemistry A 6, no. 36 (2018): 17571–78. http://dx.doi.org/10.1039/c8ta06238b.
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McMillin, Douglas W., Joseph Negri, Jake Delmore, Patrick Hayden, Melissa Ooi, Nicholas Mitsiades, Robert Schlossman, et al. "Activity of New Heat Shock Protein 90 (hsp90) Inhibitor NVP-AUY922 Against Myeloma Cells Sensitive and Resistant to Conventional Agents." Blood 110, no. 11 (November 16, 2007): 1587. http://dx.doi.org/10.1182/blood.v110.11.1587.1587.
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Abstract Context: The molecular chaperone hsp90 is a major anti-cancer therapeutic target because it regulates the function of proteins with pivotal roles in tumor cell proliferation, survival and drug resistance, including mutated/chimeric oncoproteins or oncogenic kinases/receptors. Our preclinical studies on the ansamycin hsp90 inhibitor tanespimycin (17-AAG) provided the rationale for clinical trials, either alone or in combination with the proteasome inhibitor bortezomib, for treatment of relapsed/refractory MM. In this study, we report preclinical studies of the new, non-ansamycin, hsp90 inhibitor NVP-AUY922. Methods/Results: We tested 36 human MM cell lines and observed with MTT colorimetric survival assays potent time- and dose-dependent anti-MM activity of NVP-AUY922. IC50 values were <12.5 nM for 31 of 35 cell lines, which were also more sensitive than all non-malignant cells tested with NVP-AUY922, including bone marrow stromal cells (BMSCs), immunortalized human hepatocytes and normal donor PBMCs, indicating a differential selectivity of NVP-AUY922 against neoplastic tissues vs. non-malignant cells. Importantly, MM cell lines or primary MM tumor cells resistant to dexamethasone (Dex), melphalan, immunomodulatory thalidomide derivatives (IMIDs), bortezomib or TRAIL were sensitive to NVP-AUY922. In addition, MM-1S cells constitutively over-expressing Akt were equally sensitive to NVP-AUY922 compared to their parental cell line. These anti-MM effects were rapid, as MM cells were committed to cell death within 16 hrs of exposure to only 25 nM of NVP-AUY922. NVP-AUY922 overcame the protective effects conferred to MM cells by exogenous IL-6 and IGF-1, while BMSC co-culture attenuated NVP-AUY922 activity only at doses <20 nM, as evidenced by compartment-specific bioluminescence imaging (CS-BLI). Mechanistic studies of NVP-AUY922 treatment of MM-1S cells showed early G2/M arrest followed by increased cell death, accompanied by caspase-3 and -8 cleavage (as early as 16 hrs). These events were preceded by decreased levels of Akt, B-Raf, phospho-MEK, cIAP2, and XIAP; and compensatory upregulation of hsp27 and hsp70. Given the highly single-agent activity of NVP-AUY922 at even low nM concentrations, formal statistical documentation of synergy was not observed in NVP-AUY922 combinations with conventional (e.g. Dex, doxorubicin) or novel (e.g. bortezomib) anti-MM agents. Encouragingly, no evidence of antagonism with any of these combinations was observed, indicating that NVP-AUY922 can be combined with current anti-MM agents in clinical settings. Conclusion: The new hsp90 inhibitor NVP-AUY922 has potent in vitro activity against MM cells resistant to conventional therapeutics, with selectivity for malignant compared to normal cells. Ongoing in vivo experiments and studies to identify biomarkers of pronounced sensitivity to NVP-AUY922 will help provide a framework for potential clinical trials of NVP-AUY922 in MM and other neoplasias.
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Wiedmann, M., M. Haefner, M. Niederhagen, K. Schoppmeyer, C. Wittekind, C. Moebius, J. Mossner, and K. Caca. "Treatment of pancreatic cancer with two novel histone deacetylase inhibitors NVP-LAQ824 and NVP-LBH589." Journal of Clinical Oncology 25, no. 18_suppl (June 20, 2007): 4624. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.4624.
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4624 Background: Pancreatic cancer is the fifth to sixth leading cause of cancer death in Europe and up to 90% of patients will present with locally advanced or metastatic disease. Unfortunately, little progress has been obtained from chemotherapy regimens in the past decade. Treatment with histone deacetylase inhibitors, like NVP-LAQ824 and NVP-LBH589, either alone or in combination with conventional chemotherapy may be a novel alternative. Methods: Cell-growth inhibition by NVP-LAQ824 and NVP-LBH589 was studied in vitro in 8 human pancreatic cancer cell lines by MTT assay. In addition, the anti-tumoral effect of NVP-LBH589 was studied in a chimeric mouse model. Anti-tumoral drug mechanism was assessed by immunoblotting for p21WAF-1, acH4, p42/p44, Phospho-p42/p44, AKT, Phospho- AKT, cell cycle analysis, TUNEL assay, and immunohistochemistry for MIB-1. Results: In vitro treatment with both compounds significantly suppressed the growth of all cancer cell lines (mean IC50 (6d) 0.06 and 0.03 μM, respectively) and was associated with hyperacetylation of nucleosomal histones H4, increased expression of p21WAF-1, and cell cycle arrest at G2/M-checkpoint. After 21 d, NVP-LBH589 alone reduced tumor mass in vivo by 70% and in combination with gemcitabine by 81% in comparison to placebo for cell line L3.6 pl. Further analysis of the tumor specimens revealed slightly increased apoptosis (TUNEL) and no significant reduction of cell proliferation (MIB-1). Protein levels of p42/p44, and AKT remained stable, whereas levels of Phospho-p42/p44 and Phospho-AKT increased. Conclusions: Our findings suggest that NVP-LBH589 > NVP-LAQ824 are active against human pancreatic cancer in vitro. In addition, NVP-LBH589 demonstrated significant in vivo activity and potentiated the efficacy of gemcitabine, although the precise mechanism of drug action is not yet completely understood. Therefore, further preclinical and clinical evaluation of this new drug for the treatment of pancreatic cancer is recommended. No significant financial relationships to disclose.
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Patterson-Fortin, Jeffrey, Heta Jadhav, Constantia Pantelidou, Tin Phan, Carter Grochala, Jie Hao, Junning Wang, et al. "Abstract C010: Polymerase theta inhibition synergizes with anti-PD-1 immunotherapy in BRCA2-deficient pancreatic ductal adenocarcinoma." Cancer Research 82, no. 22_Supplement (November 15, 2022): C010. http://dx.doi.org/10.1158/1538-7445.panca22-c010.
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Abstract Recently developed inhibitors of polymerase theta (POLQ) have demonstrated synthetic lethality in pre-clinical tumor models of BRCA1 and BRCA2-deficient cancers. Since other DNA repair inhibitors, including PARP inhibitors, induce changes in the tumor immune microenvironment (Pantelidou et al., Cancer Discovery, 2019), we asked whether POLQ inhibition would cause similar alterations in preclinical models of pancreatic ductal adenocarcinoma (PDAC). Initially, we generated BRCA2-deficient cells by CRISPR-mediated deletion of the BRCA2 gene in a murine KrasLSL-G12D/+;Trp53LSL-R172H/+;Pdx1-Cre;Rosa26YFP/YFP (KPCY) PDAC cell line (Li et al., Immunity, 2018). Injection of these cells into syngeneic mice generated an aggressive immunocompetent BRCA2-deficient tumor model. We next demonstrated that novobiocin (NVB), a first-in-class inhibitor of the POLQ ATPase domain (Zhou et al., Nature Cancer, 2021), induces both innate and adaptive immune responses in this model through activation of the cGAS/STING pathway. NVB exposure resulted in upregulation of micronuclei, increased cGAS/STING pathway activation, increased type I interferon expression, increased CD8+ T-cell infiltration, and local paracrine activation of dendritic cells. NVB-mediated POLQ inhibition also upregulated PD-L1 expression, correlating with increased cGAS/STING pathway activation. These NVB-induced changes were associated with antitumor activity. Whereas depletion of CD8+ T-cells compromised the efficacy of NVB, the antitumor effects of POLQ inhibition were augmented when combined with anti-PD-1 immunotherapy. Taken together, our findings demonstrate that NVB-mediated POLQ inhibition induces immune responses in a cGAS/STING-dependent manner and provides a rationale for combining POLQ inhibition with immune checkpoint blockade for the treatment of BRCA2-deficient pancreatic cancers. Citation Format: Jeffrey Patterson-Fortin, Heta Jadhav, Constantia Pantelidou, Tin Phan, Carter Grochala, Jie Hao, Junning Wang, Elizabeth A. Andrews, Jennifer L. Guerriero, Brian M. Wolpin, Ben Z. Stanger, Andrew A. Aguirre, James M. Cleary, Alan A. D'Andrea, Geoffrey I. Shapiro. Polymerase theta inhibition synergizes with anti-PD-1 immunotherapy in BRCA2-deficient pancreatic ductal adenocarcinoma [abstract]. In: Proceedings of the AACR Special Conference on Pancreatic Cancer; 2022 Sep 13-16; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2022;82(22 Suppl):Abstract nr C010.
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Liu, Xiang Ming, and Yang Wang. "Design of Red High-Definition NVD Mecha and Vibration Analysis." Applied Mechanics and Materials 26-28 (June 2010): 528–34. http://dx.doi.org/10.4028/www.scientific.net/amm.26-28.528.
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The problem considered in the paper is the structure design and modal analysis of Mecha in HD-NVD player. Regarding general DVD player, the influence on pickup from structure designs of spindle motor and base, assembly accuracy etc. have already been solved. But for HD-NVD player, as the speed of spindle motor working in the range from 3000 to 7000 r/m, so the these influence on pickup above correlative element have to be solved by complicated analysis. Through using ALGOR to design and analyze Mecha, obtained the structure dimension of base and modal characteristic, found its material property effecting greatly on the vibration of Mecha. With the increase of material rigidity in the base and motor stator, the system rigidity increases, then the vibration of system decreases. The result indicates that the Mecha designed and analyzed by ALGOR can meet the operating demand of HD-NVD player.
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Gourdeau, Lionel, William S. Kessler, Russ E. Davis, Jeff Sherman, Christophe Maes, and Elodie Kestenare. "Zonal Jets Entering the Coral Sea." Journal of Physical Oceanography 38, no. 3 (March 1, 2008): 715–25. http://dx.doi.org/10.1175/2007jpo3780.1.
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Abstract The South Equatorial Current (SEC) entering the Coral Sea through the gap between New Caledonia and the Solomon Islands was observed by an autonomous underwater vehicle (Spray glider) and an overlapping oceanographic cruise during July–October 2005. The measurements of temperature, salinity, and absolute velocity included high-horizontal-resolution profiles to 600-m depth by the glider, and sparser, 2000-m-deep profiles from the cruise. These observations confirm the splitting of the SEC into a North Vanuatu Jet (NVJ) and North Caledonian Jet (NCJ), with transport above 600 m of about 20 and 12 Sv, respectively. While the 300-km-wide NVJ is associated with the slope of the main thermocline and is thus found primarily above 300 m, the NCJ is a narrow jet about 100 km wide just at the edge of the New Caledonian reef. It extends to at least a 1500-m depth with very little shear above 600 m and has speeds of more than 20 cm s−1 to at least 1000 m. An Argo float launched east of New Caledonia with a parking depth fixed at 1000 m became embedded in the NCJ and crossed the glider/cruise section at high speed about 3 months before the glider, suggesting that the jet is the continuation of a western boundary current along the east side of the island and extends across the Coral Sea to the coast of Australia. In the lee of New Caledonia, the glider passed through a region of eddies whose characteristics are poorly understood.
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Pizzorni, C., G. Ferrari, C. Schenone, E. Gotelli, S. Paolino, A. Sulli, G. Pacini, E. Alessandri, V. Smith, and M. Cutolo. "POS0862 NAILFOLD CAPILLAROSCOPY IN UNDIFFERENTIATED AND MIXED CONNECTIVE TISSUE DISEASES." Annals of the Rheumatic Diseases 80, Suppl 1 (May 19, 2021): 686. http://dx.doi.org/10.1136/annrheumdis-2021-eular.2562.
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Background:Microvascular damage is a frequent feature in connective tissue diseases (CTDs) and can be easily detected trough nailfold videocapillaroscopy (NVC) (1,2). Mixed and Undifferentiated connective tissue diseases (MCTD and UCTD) do not show a specific and unique NVC pattern (3). However, a variety of microvascular abnormalities can occur in these two CTDs, both non-specific or specific for the scleroderma like-pattern (3-5).Objectives:To retrospectively assess and compare nailfold microangiopathy observed by NVC in MCTD and stable UCTD versus primary Raynaud’s phenomenon (PRP) (6). In addition, the aim was to correlate NVC findings with serum levels of autoantibodies (Abs) against extractable nuclear antigen (ENA) detected in UCTD.Methods:Files of fourty-six MCTD patients (Kasukawa’s criteria) (mean age 42.8±16 SD years), fourty-seven UCTD patients (mean age 47.7±16.1 SD years), fifty-one PRP (mean age 45.9±17.3 SD years) were retrospectively evaluated in the study. Among UCTD and MCTD patients 95% of both showed Raynaud’s phenomenon. Main NVC parameters (i.e. dilated capillaries, giant capillaries, microhemorrhages, abnormal shapes and number of capillaries) and related semiquantitative scale (score 0–3 for every parameter), were analyzed and compared between the two distinct CTD groups and PRP. Furthermore, ENA Abs (in particular, Ro/SSA, La/SSB, Scl70 and Jo1) were evaluated. The CTD patients were receiving different immunosuppressive treatments. Statistical analysis was performed by non-parametric tests.Results:Among UCTD group, 36% of patients showed a normal NVC pattern, 53% had non-specific NVC abnormalities and 11% had a scleroderma like-pattern. The latter was significantly more frequent in MCTD than in UCTD (p<0.001), in fact 22 out of 46 (48%) MCTD patients presented a scleroderma-like pattern. On the other hand, normal pattern or non-specific NVC abnormalities were respectively found in 9% and 43% MCTD patients. Therefore, CTD patients showing giant capillaries, abnormal shapes (i.e. angiogenesis) and lower capillary density were significantly more affected by MCTD than UCTD (p<0.001). Finally, the absolute number of capillaries was found significantly lower in MCTD versus UCTD patients (mean 7±1.7 SD vs mean 9.2±1 SD, respectively, p<0.001). Not any statistical correlation was observed between NVC parameters and specific Abs ENA in UCTD. PRP showed a normal NVC pattern in 2% and non-specific capillary abnormalities in 98%, (including dilated capillaries and microhemorrhages).Conclusion:NVC features in UCTD patients seem very close to the pattern observed in PRP (mostly non-specific capillary abnormalities), conversely in MCTD the scleroderma-like pattern was found significantly prevalent together with a significant capillary number reduction. The transition from the scleroderma-like to the scleroderma pattern (mean systemic sclerosis) is matter of actual investigation.References:[1]Cutolo M. et al. Best Pract Res Clin Rheumatol 2008; 22:1093-108.[2]Sulli A, Ann Rheum Dis. 2008;67:885-7.[3]Smith V. et al. Autoimmunity Reviews 2020; 19:102458.[4]De Holanda Mafaldo DA, et al. Lupus. 2007; 16:254–8.[5]Smith V, et al. Ann Rheum Dis 2010; 69: 1092-96.[6]Antunes M, et al. RMD Open. 2019, 26;4.Disclosure of Interests:None declared
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Boleto, G., C. Kasser, Y. Allanore, and J. Avouac. "POS0858 NAILFOLD VIDEOCAPILLAROSCOPY FINDINGS AND ASSOCIATIONS WITH ORGAN INVOLVEMENT IN MIXED CONNECTIVE TISSUE DISEASE." Annals of the Rheumatic Diseases 81, Suppl 1 (May 23, 2022): 723.1–723. http://dx.doi.org/10.1136/annrheumdis-2022-eular.1252.
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BackgroundMixed connective tissue disease (MCTD) is a rare autoimmune condition characterized by Raynaud’s phenomenon, positivity of antibodies targeting the U1 small nuclear ribonucleoprotein particle (U1 snRNP), various clinical features and potential risk of severe cardio-pulmonary involvement (interstitial lung disease, ILD, and pulmonary hypertension, PH) (1), (2).Nailfold videocapillaroscopy (NVC) is a simple, non-invasive and inexpensive imaging technique that allows a detailed assessment of skin microcirculation. NVC abnormalities have been barely described in MCTD and associations between NVC features and MCTD disease characteristics have only been partially investigated.ObjectivesTo describe the NVC patterns observed in MCTD and assess their potential association with disease characteristics.MethodsCross-sectional study based on the retrospective analysis of patients hospitalized in the department of Rheumatology, Cochin Hospital, Paris France. To be included, patients were required to fulfil at least one classification criteria used in MCTD (3). The following data were collected: demographics, clinical features, para-clinical and laboratory data, treatment, and NVC findings.Results51 patients met the inclusion criteria. Mean age was 51±12 years, 44 (86%) were women, mean disease duration was 13.8±11.1 years and 16 patients (31%) had ILD.Three different NVC patterns have been identified in our cohort (Figure 1): A) normal findings in 6 patients (11,7 %); B) non-specific organic microangiopathy in 16 patients (31,4%) and C) scleroderma pattern in 18 patients (35.3%), defined by the presence of at least 3 of the 4 following features: at least 1 giant capillary, decreased capillary density (<7/mm), avascular areas and disorganization of capillary bed architecture (4).Scleroderma pattern was associated with clinical features of systemic sclerosis: skin sclerosis (9/18 vs. 5/33; p=0.008) and digital ulcers (6/18 vs. 2/31; p=0.017). Conversely, no association was observed between the normal or the non-specific NVC pattern and disease specific characteristics.Interestingly, we observed, a significant reduction in the number of capillaries in patients with ILD (4.80±1.87 vs. 6.03±1.47; p=0.039), and patients with severe reduction of capillary density (≤4/mm) were more likely to have ILD (5/7 vs. 5/33; p=0.002). Neoangiogenesis was also more frequent in patients with ILD (6/13 vs. 4/27; p=0.034).Multivariate logistic regression analysis showed that the association between severe reduction of capillary density and ILD was observed independently of the presence of a scleroderma NVC pattern and skin fibrosis.Figure 1.ConclusionWe identified three main NVC patterns in MCTD patients. The scleroderma NVC pattern was associated with clinical scleroderma characteristics whereas non-specific microangiopathy and normal NVC were not associated with a specific phenotype. Moreover, severe capillary loss was independently associated with the presence of ILD. These data suggest that NVC may be helpful for disease risk stratification in MCTD, and that NVC findings, and particularly severe capillary loss, may be a warning for the presence of ILD.References[1]Sharp GC, Irvin WS, Tan EM, Gould RG, Holman HR. Mixed connective tissue disease--an apparently distinct rheumatic disease syndrome associated with a specific antibody to an extractable nuclear antigen (ENA). Am J Med. févr 1972;52(2):148‑59.[2]Gunnarsson R, Hetlevik SO, Lilleby V, Molberg Ø. Mixed connective tissue disease. Best Pract Res Clin Rheumatol. févr 2016;30(1):95‑111.[3]John KJ, Sadiq M, George T, Gunasekaran K, Francis N, Rajadurai E, et al. Clinical and Immunological Profile of Mixed Connective Tissue Disease and a Comparison of Four Diagnostic Criteria. Int J Rheumatol. 2020;2020:9692030.[4]Boulon C, Devos S, Mangin M, Decamps-Le Chevoir J, Senet P, Lazareth I, et al. Reproducibility of capillaroscopic classifications of systemic sclerosis: results from the SCLEROCAP study. Rheumatol Oxf Engl. 1 oct 2017;56(10):1713‑20.Disclosure of InterestsGonçalo Boleto: None declared, Camille Kasser: None declared, Yannick Allanore Speakers bureau: YA reports personal fees from Actelion, Bayer, BMS, Boehringer, Curzion, Inventiva, Roche and Sanofi and research grants from Inventiva, Sanofi and Alpine Immunosciences., Jerôme Avouac: None declared
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Negrin, Zenaida, Yoslainy Valdés, Tania Pouron, Edell López, and Keyller Borges. "A novel 2-(2-methyl-2-nitrovinyl)-furan ectoparasitic drug: physicochemical characterization and determination of the raw material by gas chromatography mass spectrometry." Open Chemistry 11, no. 4 (April 1, 2013): 594–603. http://dx.doi.org/10.2478/s11532-012-0194-z.
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Abstract2-(2-methyl-2-nitrovinyl)-furan (NVF) has recently been synthesized and the pharmaceutical industry interest in this compound has grown due to its antibacterial, fungicidal and anti-ectoparasitic activities. Therefore, the physicochemical characterization of new drug was conducted. In addition, two rapid, simple and suitable GC methods were developed for determination of NVF. Analyses were carried out with an Agilent DB-5ms capillary column (60 m × 0.25 mm i.d., 0.25 µm film thickness). The GC-FID analysis employed splitless mode of injection, oven/injector/detector temperature of 160/230/280°C and nitrogen carrier at the flow of 5.0 mL min−1. The GC-MS analysis employed splitless mode of injection, helium carrier at the flow of 1.5 mL min−1, column temperature program with 2 min at 100°C, ramp at 50°C min−1 to 260°C and injector and detector temperature of 250 and 290°tC, respectively. The MS conditions were ionization voltage, 70 eV; mass range, m/z 40–350; and ion source temperature, 200°C. The analysis time took less than 6 min. The results obtained in the validation of the methods suggest that these methods are economic, precise, accurate and linear over the range of analysis. The methods were successfully employed during the synthesis of NVF in order to ensure the quality of the raw material.
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Ferrari, G., S. Paolino, A. Sulli, C. Pizzorni, G. Pacini, E. Gotelli, A. Lercara, V. Smith, and M. Cutolo. "AB0408 DOES THE PRESENCE IN THE SERUM OF ANTIPHOSPHOLIPID ANTIBODIES CORRELATE WITH SPECIFIC/NON SPECIFIC CAPILLAROSCOPIC ABNORMALITIES?" Annals of the Rheumatic Diseases 79, Suppl 1 (June 2020): 1504.2–1504. http://dx.doi.org/10.1136/annrheumdis-2020-eular.4881.
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Background:Antiphospholipid syndrome (APS) is a systemic autoimmune disease characterized by specific vascular and obstetric manifestations and by antiphospholipid antibodies (aPL) positivity [1].To date, little is known regarding nailfold videocapillaroscopy (NVC) alterations in APS patients and in asymptomatic aPL-carriers, non-specific abnormalities being the most frequently reported [2,3,4].Objectives:To retrospectively analyze NVC alterations in APS patients and in asymptomatic aPL-carriers and to correlate NCV alterations with both clinical manifestations and serum aPL profile.Methods:Thirty-five aPL positive patients having received at least one NCV investigation (mean age 47 years, range 16-81, 31 female and 4 male) were retrospectively included in the study. For each patient complete medical history was collected with a particular attention to past vascular thrombosis and pregnancy morbidity. Patients were classified as affected by APS according to the updated Sapporo classification criteria [5]. Lupus anticoagulant (LAC), IgM and IgG anti-cardiolipin antibodies (ACL) and IgM and IgG anti-Beta2 Glycoprotein 1 (anti-B2GP1) were assessed in each patient according to the recommended procedures [5]. NCV parameters were analyzed in each patient, with a particular interest to hemorrhages or nailfold bed-parallel hemosiderin deposits (“comb-like”hemorrhages) presence [2,6]. Statistical analysis was performed by parametric and non-parametric tests.Results:Seventeen patients (mean age 49 years, range 16-81 years) were asymptomatic aPL-carriers and 18 (mean age 46 years, range 26-71 years) were affected by APS. Within APS patients, 16 had a history of vascular thrombosis and 2 had pregnancy morbidity; in 6 patients APS was secondary to other autoimmune rheumatologic conditions (3 to Systemic Lupus Erythematosus, 2 to vasculitides and 1 to Mixed Connective Tissue Disease).Among the total number of aPL-carriers and APS patients six patients showed a normal NVC pattern, 24 patients had non-specific NVC abmormalities and 5 patients had a “scleroderma-like” pattern. Interestingly, NCV microhemorrhages were significantly more frequent in APS patients than in asymptomatic aPL-carriers, both in score and in absolute (p=0.05 andp=0.04, respectively). Particularly, in APS patients “comb-like”hemorrhages had a statistically significant higher prevalence than isolated hemorrhages (p=0.03). Dilated capillaries score was significantly higher in APS patients than in asymptomatic aPL-carriers (p=0.01).Not any statistically significant difference was observed regarding other capillary parameters (score of giant capillaries, loss of capillaries, or anormal shpaes, i.e. angiogenesis). Not any statistical correlation was observed between NVC parameters and different aPL profile.Conclusion:The study shows that the total number of microhemorrhages and in particular the“comb-like”subtype, are significantly the most frequent specific abnormalities in APL patients when compared to asymptomatic aPL carriers. The presence of the “scleroderma like” NVC pattern may suggest a concomitant overlap syndrome. Not any correlation was found between aPL profile and other NVC parameters. Further studies need to develop a more specific APS NVC pattern for APS patients.References:[1]Tektonidou MG, et al RMD Open 2019; 5(1);[2]Cutolo M, Elsevier 2010, pp141-143;[3]Candela M, et al.1998:444-9;[4]Aslanidis S, et al. Clin Exp Rheumatol 2011, 29:307-9;[5]Miyakis S, et al. J Thromb Haemost 2006, 4:295–306;[6]Cutolo M, et al Best Pract Res Clin Rheumatol 2008, 22:1093-108Disclosure of Interests: :Giorgia Ferrari: None declared, Sabrina Paolino: None declared, Alberto Sulli Grant/research support from: Laboratori Baldacci, Carmen Pizzorni: None declared, Greta Pacini: None declared, Emanuele Gotelli: None declared, Adriano Lercara: None declared, Vanessa Smith Grant/research support from: The affiliated company received grants from Research Foundation - Flanders (FWO), Belgian Fund for Scientific Research in Rheumatic diseases (FWRO), Boehringer Ingelheim Pharma GmbH & Co and Janssen-Cilag NV, Consultant of: Boehringer-Ingelheim Pharma GmbH & Co, Speakers bureau: Actelion Pharmaceuticals Ltd, Boehringer-Ingelheim Pharma GmbH & Co and UCB Biopharma Sprl, Maurizio Cutolo Grant/research support from: Bristol-Myers Squibb, Actelion, Celgene, Consultant of: Bristol-Myers Squibb, Speakers bureau: Sigma-Alpha
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Paolino, S., E. Gotelli, A. Casabella, F. Cattelan, C. Schenone, M. Patanè, G. Pacini, et al. "SAT0337 EVALUATION OF BODY COMPOSITION AND BONE STATUS ACCORDING TO MICROVASCULAR INVOLVEMENT IN SYSTEMIC SCLEROSIS PATIENTS." Annals of the Rheumatic Diseases 79, Suppl 1 (June 2020): 1114.3–1114. http://dx.doi.org/10.1136/annrheumdis-2020-eular.5693.
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Background:Systemic sclerosis (SSc) is a complex autoimmune connective tissue disease, characterized by autoimmune inflammatory microvascular damage with progressive loss of capillaries, fibrosis and ischemia of skin and internal organs. (1) Nailfold videocapillaroscopy (NVC) is a safe toll for early diagnosis of SSc, it identify morphological changes of vessel that are predictive for clinical disease progression and organ involvement.(2) About clinical complication the loss of bone mass and body composition abnormalities, particularly muscle mass and strength loss (sarcopenia), are recognized in advanced disease.(3)Objectives:To evaluated in SSc patients, the body composition and the bone status according to the microvascular condition, assessed and scored by nailfold videocapillaroscopy (NVC, “Early”,”Active”,”Late” patterns).Methods:Body composition and bone mineral density (BMD) were assessed by DEXA in 35 female SSc patients classified according to the 2013 EULAR/ACR criteria and 32 sex-matched healthy subjects. Clinical, laboratory, body composition and bone parameters were analysed according to the different NVC patterns. Means were compared by the Student’s t test or one way analysis of variance; medians were compared by the Kruskall Wallis test; and frequencies by the chi square test.Results:Higher prevalence of vertebral (26.4%vs 9.3%) and femoral (32.3% vs 9.3%) osteoporosis (OP) was found in SSc. Particularly SSc patients with “Late” NVC pattern showed a significantly higher prevalence of vertebral (p=0.018) and femoral OP (p=0.016). Regional assessment of bone mass (BM) in 7 different body areas showed a significant lower BMD only at the total spine (P=0.008) and femoral neck (p=0.027) in advanced microvascular damage. Patients with “Late” NVC pattern showed lower whole body lean mass (LM) compared to “Early” and “Active” NVC patterns, particularly at upper limbs. To note, in all body sites, BMD correlate with LM and BMC according to NVC pattern severity.Conclusion:SSc patients with most severe microvascular damage show a significantly altered body composition and bone status suggesting a strong link between microvascular failure and associated muscle/bone sufference.References:[1]Cutolo M et al. Expert Rev Clin Immunol 2019; 15(7):753-64[2]Cutolo M et al. Clin Rheumatol 2019; 38(9):2293-7[3]Corallo C et al. Rheumatol Int 2019;39(10):1767-75.Disclosure of Interests:Sabrina Paolino: None declared, Emanuele Gotelli: None declared, Andrea Casabella: None declared, Francesco Cattelan: None declared, Carlotta Schenone: None declared, Massimo Patanè: None declared, Greta Pacini: None declared, Carmen Pizzorni: None declared, Alberto Sulli Grant/research support from: Laboratori Baldacci, Vanessa Smith Grant/research support from: The affiliated company received grants from Research Foundation - Flanders (FWO), Belgian Fund for Scientific Research in Rheumatic diseases (FWRO), Boehringer Ingelheim Pharma GmbH & Co and Janssen-Cilag NV, Consultant of: Boehringer-Ingelheim Pharma GmbH & Co, Speakers bureau: Actelion Pharmaceuticals Ltd, Boehringer-Ingelheim Pharma GmbH & Co and UCB Biopharma Sprl, Maurizio Cutolo Grant/research support from: Bristol-Myers Squibb, Actelion, Celgene, Consultant of: Bristol-Myers Squibb, Speakers bureau: Sigma-Alpha
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Lazcano-Gomez, Gabriel, Jeffrey R. Soohoo, Anne Lynch, Levi N. Bonell, Karina Martinez, Mauricio Turati, Roberto Gonzalez-Salinas, Jesus Jimenez-Roman, and Malik Y. Kahook. "Neovascular Glaucoma: A Retrospective Review from a Tertiary Eye Care Center in Mexico." Journal of Current Glaucoma Practice 11, no. 2 (2017): 48–51. http://dx.doi.org/10.5005/jp-journals-10028-1222.
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ABSTRACT Aim To describe the demographic characteristics, ocular comorbidities, and clinical outcomes of patients with neovascular glaucoma (NVG) and to determine the number of patients who returned for a follow-up eye examination. Materials and methods We examined the clinical data of patients with NVG, who attended a glaucoma clinic between July 2010 and November 2014. We collected information on the demographic characteristics of the patients to include the level of education, ocular comorbidities, NVG stage, visual acuity, glaucoma medications, intraocular pressure (IOP), and the number of patients who had a follow-up ocular examination at month 1, 3, 6, and 12. Results Data from 350 patients (473 eyes) with NVG were collected. We found 91% of the cohort had proliferative diabetic retinopathy (PDR). We found blindness in both or one eye in 14% and 31% of the cohort respectively. Low vision was found in both or one eye in 14% and 32% of the eyes respectively. By 6 months follow-up, only 32% of the patients were seen at our clinic and by 12 months follow-up, this number decreased to 15%. Around 60% of the patients were on no IOP lowering drugs at the first visit. We found 53% of the cohort had an incomplete elementary school education. Conclusion The results suggest that advanced NVG is a significant ocular problem for patients referred to our clinic with just over half of the patients presenting as blind. We also found that several socioeconomic factors that had an important role in the development of PDR and NVG, specifically, educational status. Clinical significance We described the characteristics of a large cohort of patients with very advanced NVG, reflecting the fact that the strict control of the underlying disease must be the main goal of the Mexican national health system. How to cite this article Lazcano-Gomez G, Soohoo JR, Lynch A, Bonell LN, Martinez K, Turati M, Gonzalez-Salinas R, Jimenez-Roman J, Kahook MY. Neovascular Glaucoma: A Retrospective Review from a Tertiary Eye Care Center in Mexico. J Curr Glaucoma Pract 2017;11(2):48-51.
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Mendes, Luciano B., Ilda F. F. Tinoco, Nico W. M. Ogink, Keller S. O. Rocha, Jairo A. Osorio S., and Marilú S. Santos. "Ammonia emissions from a naturally and a mechanically ventilated broiler house in Brazil." Revista Brasileira de Engenharia Agrícola e Ambiental 18, no. 11 (November 2014): 1179–85. http://dx.doi.org/10.1590/1807-1929/agriambi.v18n11p1179-1185.
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This study was conducted with the aim of monitoring NH3 emissions from a mechanically and a naturally ventilated broiler house (MVB and NVB, respectively) and calculate their ammonia emission factors (fNH3). Bird stocking density was 13.5 and 11.1 birds m-2 for the MVB and NVB, respectively. The marketing age was 43 days and bedding consisted of dried coffee husks in its first time of use. Ventilation rates were calculated with the metabolic carbon dioxide mass balance method. Values of fNH3 were 0.32 ± 0.10 and 0.27 ± 0.07 g bird-1 d-1 for the MVB and NVB, respectively, and are in agreement to what was presented in other studies performed under similar conditions. The fNH3 estimated on yearly basis was 58 g bird-place-1 year-1. It was concluded that the different types of ventilation system between the studied broiler barns did not significantly affect emissions in the modeling process. The results obtained help providing reliable methodology for the determination of a solid database on NH3 emission factors for tropical conditions that can be used for future inventories, when performed in a sufficient number of barns that is representative for the Brazilian scenario.
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Zaremba, Sigitas, Renatas Aškinis, Agota Piščikaitė, Giedrė Smailytė, and Saulius Cicėnas. "Stemplės vėžio gydymo rezultatai Nacionaliniame vėžio institute 2008–2017 metais." Lietuvos chirurgija 18, no. 2 (July 9, 2019): 91–100. http://dx.doi.org/10.15388/lietchirur.2019.18.10.
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Įvadas. Stemplės vėžys (SV) yra aštunta pagal dažnumą onkologinė liga ir šešta mirties dėl vėžio priežastis pasaulyje. Nepaisant pažangos šią ligą diagnozuojant ir gydant, bendrasis 5-erių metų reliatyvus išgyvenamumas siekia 15–25 proc. Straipsnio tikslas – apžvelgti 2008–2017 m. Nacionaliniame vėžio institute (NVI) gydyto SV atvejų gydymo patirtį, įvertinti operacinio, konservatyvaus ir paliatyvaus gydymo rezultatus, nustatyti ligonių išgyvenamumą. Tyrimo metodika. 2008–2017 m. NVI gydėsi 512 pacientų, sergančių SV. Ligoniams taikytas operacinis, paliatyvus operacinis, chemospindulinis, spindulinis, chemoterapinis ar simptominis gydymas. Statistinė analizė atlikta naudojant STATA 11 statistinę programinę įrangą. Rezultatai. Tirtų pacientų amžiaus vidurkis – 61,8 metai. Iš 512 tiriamųjų 63 buvo moterys (12,3 %), 449 – vyrai (87,7 %). I stadijos vėžiu sirgo 25 (4,9 %), II stadijos vėžiu – 74 (14,4 %), III stadijos vėžiu – 258 (50,4 %), IV stadijos vėžiu – 155 (30,3 %) pacientai. Dažniausia morfologija – plokščialąstelinė karcinoma (445 atvejai, 86,9 %), adenokarcinoma – 48 atvejai (9,4 %). Operuoti radikaliai – 75 (14,6 %), paliatyviai – 271 ligonis. Chemospindulinis gydymas taikytas 97 (19,0 %) ligoniams, spindulinis – 81 (15,8 %), chemoterapinis – 111 (21,7 %), simptominis – 148 (28,9 %). Po radikalių operacijų komplikacijų dažnis – 46,17 proc., mirtingumas – 10,67 proc. Dažniausia komplikacija – fistulė. Bendrasis reliatyvus išgyvenamumas: vienus metus – 33,91 proc., penkerius metus – 9,0 proc. Atsižvelgiant į gydymo rūšį, reliatyvusis 5-erių metų išgyvenamumas po radikalių operacijų siekė 26,53 proc., po chemospindulinio gydymo – 17,95 proc., po radioterapinio gydymo – 5,36 proc., po chemoterapinio gydymo – 1,92 proc., po simptominio gydymo – 1,92 proc. Išvados: Po radikalaus SV operacinio gydymo pooperacinių komplikacijų dažnis siekė 46,17 proc., mirtingumas – 10,67 proc. Po paliatyvaus operacinio gydymo komplikacijų dažnis siekė 4,5 proc., mirtingumas – 0,7 proc. NVI gydytų SV sergančiųjų bendrasis 5-erių metų reliatyvus išgyvenamumas – 9,0 proc. Reikšmingos įtakos išgyvenamumui turėjo taikytas gydymas, diagnozuotos ligos stadija ir pirminio naviko dydis.
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Sambataro, G., D. Sambataro, F. Pignataro, N. Del Papa, M. Colaci, L. Malatino, A. Libra, et al. "FRI0260 NAILFOLD VIDEOCAPILLAROSCOPY IS A USEFUL TOOL TO RECOGNIZE SYSTEMIC SCLEROSIS AND IDIOPATHIC INFLAMMATORY MYOPATHIES IN INTERSTITIAL LUNG DISEASE PATIENTS." Annals of the Rheumatic Diseases 79, Suppl 1 (June 2020): 714.3–714. http://dx.doi.org/10.1136/annrheumdis-2020-eular.4724.
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Background:Nailfold Videocapillaroscopy (NVC) is an essential tool for the assessment of Raynaud’s Phenomenon (RP) among the Scleroderma Spectrum Disorders (SSDs). Recently, NVC abnormalities have been associated with a diagnosis of Idiopathic Inflammatory Myopathies (IIMs), independently of the presence of RP (1). Moreover, both SSDs and IIMs are commonly associated with Interstitial Lung Disease (ILD), which is the main cause of mortality in these conditions.Objectives:To verify whether NVC may allow a better diagnostic classification in a cohort of patients with ILD followed by pulmonologists.Methods:361 patients affected by ILD were prospectively enrolled in a 30-months observational study. All these patients were clinically evaluated by rheumatologists and pulmonologists together and performed general blood tests, autoantibody research, chest High-Resolution Computed Tomography and NVC. The latter was considered positive in the presence of avascular areas or giant capillaries, and also the presence of Bushy Capillaries (BCs) was recorded.Results:NVC was positive in 17.7% of ILD patients, and a third of these patients did not present RP. Patients with NVC abnormalities had a diagnosis of definite Connective Tissue Disease in 78.1% of cases. NVC resulted decisive in 25% of patients with a final diagnosis of Systemic Sclerosis according to the ACR/EULAR 2013 criteria. The presence of BCs and/or NVC+ in ILD patients with normal serum levels of muscular enzymes was associated with amyopathic IIM (BCs= OR 3.9, 95CI 1.05-14.38, p=0.04; NVC+= OR 5, 95CI 1.29-19.3 p=0.02; BCs and/or NVC+= OR 5.41 95CI 1.24-23.48 p=0.02), regardless the presence of RP.Conclusion:NVC proved to be a valid tool in the correct assessment of ILD patients secondary to SSDs and amyopathic IIMs. Therefore, it could be considered in the diagnostic evaluation of patients affected by ILD, regardless of the presence of RP.References:[1]Sebastiani M et al. Nailfold Capillaroscopy Characteristics of Antisynthetase Syndrome and possible clinical associations: results of a multicenter International Study.J Rheumatol2019; 46: 279-284Table 1.Comparison between ILD patients with and without NVC positivity..ItemsNVC+ patientsNVC- patientspNumber64297Mean Age (±SD)61.4±13.367.8±9.80.001Female%65.649.10.01RP%68.717.1<0.0001HRCT patterns%NSIP51.536.80.02OP3.18n.s.UIP-like31.1544.8n.s.DIP1.53.4n.s.LIP1.50.4n.s.Indeterminate10.96.7n.s.Final Diagnosis%SSDs45.31<0.0001IIMs256.4<0.0001Other CTDs6.213.5n.s.IPAF10.919.5n.s.IPF7.827.90.0007Legend:DIP: Desquamative Interstitial Pneumonia; HRCT: High Resolution Computed Tomography; IIMs: Idiopathic Inflammatory Myopathies, Poly/dermatomyositis, antisynthetase syndrome; IPAF: Interstitial Pneumonia with Autoimmune Features; IPF: Idiopathic Pulmonary Fibrosis; LIP: Lymphocytic Interstitial Pneumonia; NSIP: Nonspecific Interstitial Pneumonia; OP: Organising Pneumonia; RP: Raynaud’s Phenomenon; SSDs: Scleroderma Spectrum Disorders, Systemic Sclerosis + Mixed Connective Tissue Disease; UIP: Usual Interstitial Pneumonia.Disclosure of Interests:Gianluca Sambataro: None declared, Domenico Sambataro: None declared, Francesca Pignataro: None declared, Nicoletta Del Papa: None declared, Michele Colaci: None declared, Lorenzo Malatino: None declared, Alessandro Libra: None declared, Fabio Pino: None declared, Sebastiano Emanuele Torrisi Speakers bureau: Boehringer Ingelheim; F. Hoffmann-La Roche Ltd., Stefano Palmucci Consultant of: Boehringer Ingelheim, Speakers bureau: Boehringer Ingelheim, Delphi International Srl, and F. Hoffmann-La Roche Ltd., Lorenzo Cavagna: None declared, Carlo Vanchieri Grant/research support from: F. Hoffmann-La Roche Ltd., Consultant of: AstraZeneca, Boehringer Ingelheim, Chiesi, F. Hoffmann-La Roche Ltd., and Menarini, Speakers bureau: AstraZeneca, Boehringer Ingelheim, Chiesi, F. Hoffmann-La Roche Ltd., and Menarini
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BARBOSA, WEMERSON SAULO DA SILVA, GUILHERME BASTOS LYRA, IVOMBERG DOURADO MAGALHÃES, RICARDO ARAÚJO FERREIRA JÚNIOR, IÊDO TEODORO, and JOSÉ LEONALDO DE SOUZA. "COMPONENTES DE PRODUÇÃO DE SOJA NO TABULEIRO COSTEIRO DE ALAGOAS EM CULTIVOS DE SEQUEIRO E IRRIGADO." IRRIGA 26, no. 4 (December 22, 2021): 906–23. http://dx.doi.org/10.15809/irriga.2021v26n4p906-923.
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COMPONENTES DE PRODUÇÃO DE SOJA NO TABULEIRO COSTEIRO DE ALAGOAS EM CULTIVOS DE SEQUEIRO E IRRIGADO WEMERSON SAULO DA SILVA BARBOSA1; GUILHERME BASTOS LYRA2; IVOMBERG DOURADO MAGALHÃES3; RICARDO ARAÚJO FERREIRA JÚNIOR2; IÊDO TEODORO2; JOSÉ LEONALDO DE SOUZA2 1Engenheiro Agrônomo, Doutor em Produção Vegetal- Professor substituto do curso de Agronomia da Universidade Federal do Oeste da Bahia - UFOB. Campus de Barra. Avenida 23 de Agosto, S/N, Assunção, Barra - BA, CEP: 47100-000 Barra - Bahia. E-mail: agrowssb@gmail.com 2Professor Doutor do curso Agronomia da Universidade Federal de Alagoas –UFAL- Campus de Engenharias e Ciências Agrárias– BR-104, Km 85, S/N, CEP: 57100-000- Rio Largo - Alagoas. E-mail: gbastoslyra@gmail.com, ricardo_ceca@hotmail.com, iedoteodoro@gmail.com, leonaldojs@yahoo.com.br 3Doutor em Produção Vegetal- Universidade Federal de Alagoas –UFAL- Campus de Engenharias e Ciências Agrárias– BR-104, Km 85, S/N, CEP: 57100-000- Rio Largo - Alagoas. E-mail: ivomberg31@hotmail.com 1 RESUMO Objetivou-se comparar os componentes de produção de cultivares de soja sob irrigação suplementar e em sequeiro. O delineamento experimental foi em blocos casualizados com cinco repetições e seis cultivares de soja (M 6210, M 6410, BMX-Potência, AS 3730, M 8349 e BRS-9383). Foram analisadas: altura de inserção da primeira vagem (AIPV, cm), número de vagens por planta (NVP, Unid.), massa de mil grãos (MMG, g), índice de colheita (IC), produtividade de água (PA, kg m-3) e rendimento de grãos (RG, kg ha-1). Nos cultivos irrigados, os componentes de produção variaram: AIPV de 13 a 19 cm; NVP de 43 a 118 Unid.; MMG de 147 a 207 g; IC de 17 a 45; PA de 0,80 a 1,28 kg m-3 e RG de 3.871 a 6.198 kg ha-1. Enquanto em sequeiro, a AIPV variou de 5,85 a 16,80 cm; o NVP de 28 a 72 Unid.; a MMG de 83 a 176 g; a PA de 0,55 a 0,86 kg m-3 e o RG de 2.328 a 3.600 kg ha-1. Somente o IC foi maior nos cultivos em sequeiro, variando de 16,80 a 52. Verifica-se, portanto, que o desempenho dessas seis cultivares de soja foi maior sob regime de irrigação. Palavras-chave: genótipos, grãos, Glycine max., irrigação. BARBOSA, W. S. S.; LYRA, G. B.; MAGALHÃES, I. D.; FERREIRA JÚNIOR, R. A.; TEODORO, I.; SOUZA, J. L. PRODUCTION COMPONENTS OF SOYBEAN IN THE COASTAL TABLELANDS OF ALAGOAS IN RAINFED AND IRRIGATED CROPS 2 ABSTRACT The objective was to compare the production components of soybean cultivars under supplemental irrigation and rainfed conditions. The experimental design was randomized blocks with five replications and six soybean cultivars (M 6210, M 6410, BMX-Potência, AS 3730, M 8349, and BRS-9383). The height of the first pod insertion (HFPI, cm), number of pods per plant (NPP, Units), thousand grain mass (TGM, g), harvest index (HI), water productivity (WP, kg m-3), and grain yield (GY, kg ha-1) were analyzed. In irrigated crops, the production components varied HFPI from 13 to 19 cm; NPP from 43 to 118 units; TGM from 147 to 207 g; HI from 17 to 45; WP from 0.80 to 1.28 kg m-3, and GY from 3,871 to 6,198 kg ha-1. While in rainfed conditions, the HFPI ranged from 5.85 to 16.80 cm; NPP from 28 to 72 units; TGM from 83 to 176 g; WP from 0.55 to 0.86 kg m-3, and GY from 2,328 to 3,600 kg ha-1. Only the HI was higher in rainfed crops, ranging from 16.80 to 52. It can be seen; therefore, that the performance of these six soybean cultivars was higher under irrigation. Keywords: genotypes, grains, Glycine max., irrigation.
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Collée, E., E. Van den Berg, E. Visch-Brink, C. Dirven, A. Vincent, and D. Satoer. "OS06.7.A Underlying mechanisms of verbal fluency in glioma patients: language or executive functioning?" Neuro-Oncology 24, Supplement_2 (September 1, 2022): ii15. http://dx.doi.org/10.1093/neuonc/noac174.045.
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Abstract Background Glioma patients often suffer from cognitive deficits, such as in language and executive functioning (EF). A sensitive test to identify these deficits is verbal fluency (VF). In VF, participants are asked to generate as many unique words within a given semantic category (category fluency - CF) or starting with a given letter (letter fluency - LF) within one minute. While both language and EF play a role in VF, the relative contribution of both domains remains unclear. We aim to retrospectively investigate performance on VF and, for the first time, on Non-Verbal Fluency (NVF), requiring to connect 5 points in different patterns within three minutes. Additionally, we aim to explore the influence of language and EF on VF performance in glioma patients. Materials and Methods 69 adults with gliomas in eloquent areas underwent a comprehensive neuropsychological test-battery at 1-2 months before surgery (T1) and 3 (T2) and 12 months (T3) after surgery. The protocol consisted of VF (CF: animals, professions, LF: D-A-T/K-O-M), language tests (Repetition (Akense Afasie Test), Token Test, Boston Naming Test) and EF tests (Five Point Test, Trail Making Test, Stroop Colour-Word Test, Wisconsin Modified Card Sorting Task). VF and NVF performance were compared 1) to healthy population using one sample t-tests/Wilcoxon rank-tests (all patients), and 2) between T1, T2, and T3 with paired sample t-tests/Wilcoxon rank-tests (31 patients). Linear regression analyses were conducted to investigate the predictive value of language and EF on VF for all patients. Results Preoperatively, patients were impaired on all VF tasks. Especially CF profession performance was more prone to deterioration after surgery at T2 and T3. In contrast, NVF was intact and even improved after surgery (T1-T3, T2-T3). NVF (p = .032, EF) was a predictor for CF animals while the Token Test (p = .023, language) was a predictor for CF professions. Both NVF (p = .032, EF) and the Token Test (p = .004, language) were predictors for LF. Conclusion First, glioma patients were preoperatively impaired in VF but were preserved in NVF, as is in line with the literature. Secondly, CF professions appeared to be particularly sensitive to detect long-term postoperative decline. Thirdly, the main result of this study is that both language and EF mechanisms appeared to be responsible for VF performance in glioma patients. These findings are useful for interpreting VF impairment in glioma patients and for therapeutic interventions, suggesting a combination of language and EF training materials.
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Yi, Guo Bin, Ying Wu, and Ping Ke Ai. "Reverse Atom Transfer Radical Polymerization of N-Vinylpyrrolidone in Bulk Catalyzed by AIBN)/FeCl3/ PPh3." Applied Mechanics and Materials 295-298 (February 2013): 3–7. http://dx.doi.org/10.4028/www.scientific.net/amm.295-298.3.
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The reverse atom transfer radical polymerization (RATRP) of N-vinylpyrrolidone (NVP) using azobisisobutyronitrile (AIBN)/FeCl3/triphenylphosphine(PPh3) as the initiating system, was successfully carried out in bulk at 80°C. Plots of In ([M]0/[M]) vs time and molecular weight evolution vs monomer conversion presented a linear dependence and the polymerization was proved to accord with the first-order kinetics. After 10 hours’ reaction, the monomer conversion was up to 84%. Gel permeation chromatography (GPC) was used in testing the molecular weight of polymer and molecular weight distribution, the results showed that polymer molecular weight distribution was as low as 1.018 (Mn=3288 g/mol). Moreover, the resultant polymer was characterized by 1H-NMR, 13C-NMR spectroscopy and Pyrolysis GC-MS, and the results showed that the polymerization mechanism is consistent with RATRP.
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Pinto, Marcos Vinícius Barros, Francisco Eduardo Torres, Angelita dos Santos Zanuncio, Paulo Eduardo Teodoro, Gabriele Gonçalves de Mendonça, and Denise Prevedel Capristo. "Seleção de cultivares de soja na região do ecótono cerrado-pantanal." Research, Society and Development 9, no. 7 (May 21, 2020): e434973823. http://dx.doi.org/10.33448/rsd-v9i7.3823.
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O objetivo deste trabalho foi avaliar o desempenho de dezesseis cultivares de soja e indicar aqueles com melhor adaptação para o cultivo na região de Aquidauana, MS. O trabalho foi realizado na área experimental da Universidade Estadual de Mato Grosso do Sul, Unidade de Aquidauana, no ano agrícola 2014/2015, em sistema plantio convencional. O delineamento experimental utilizado foi o inteiramente casualizado, com 16 tratamentos (RR: 97R21, 97R71, 97R73, 97Y07, AS3610, AS3730, AS3797, B4184, B4377, CD238, MOSOY6410, P98Y11, POTÊNCIA, SYN1163, SYN13671 e SYN9070) e 4 repetições. As parcelas consistiram de sete linhas com 5 m de comprimento, com espaçamento de 0,45 m entre linhas e densidade de 15 plantas por metro. Foram avaliados os caracteres agronômicos altura de planta na maturação, altura de inserção de primeira vagem, número de ramificações, número de vagens por planta, número de grãos por vagens, massa de cem grãos e produtividade de grãos. Após a coleta dos dados foi verificado o ajuste dos erros pelo teste de Liliefors e a homogeneidade das variâncias pelo teste de Bartlett, e os dados foram submetidos a analise de variância e ao agrupamento de médias pelo teste de Skott-Knott á 5% de probabilidade. Conclui-se que a cultivar B4377 obteve o melhor desempenho analisando as variáveis AP, AIV, NR e NVP em conjunto. As cultivares que obtiveram melhor desempenho analisando as variáveis NGV, MCG e PROD em conjunto foram AS3730 e P98Y11. Altura de planta NVP e MCG não influenciaram na melhor produtividade de grãos. Considerando a produtividade de grãos, todos os materiais avaliados podem ser recomendados para a região.
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Zhu, Ming, Weiwei Han, Yang Ling, Qiufeng Qi, Yaping Zhang, Yun Peng, Lin Chen, Yanqing Bao, and Yongping Liu. "Preliminary Study on the In Vitro Antitumor Effects of Nidus Vespae on Gastric Cancer." Evidence-Based Complementary and Alternative Medicine 2021 (June 7, 2021): 1–9. http://dx.doi.org/10.1155/2021/1549359.
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Objective. The aim of this study was to investigate the in vitro antitumor effects of Nidus Vespae on gastric cancer and its ability to promote immune function. Methods. Cell viability was detected by the Cell Counting Kit-8 (CCK-8) assay. Cell cycle distribution and apoptosis were detected using flow cytometry. The THP-1 human monocytic cell line was used as a source of monocytic effector cells for analyzing proliferation and dendritic cell (DC) induction. Enzyme-linked immunosorbent assay was used to detect cytokine production, and multicolor flow cytometry was used to study the phenotype and functionality of THP-1 DCs. Results. A high concentration (>10 mg/mL) of Nidus Vespae decoction (NVD) inhibited SGC-7901 gastric cancer cell growth by inducing G2/M cell cycle arrest and apoptosis. However, a low concentration (≤10 mg/mL) of NVD significantly increased the proliferative ability of THP-1 in serum-containing medium and caused an increase in dendritic protrusions with the typical morphology of DCs compared to the negative control in serum-free medium. The THP-1 DCs had significantly increased expression of cluster of differentiation 11c (CD11c), CD40, CD80, CD83, and CD86, as well as secretion of tumor necrosis factor-alpha. Furthermore, the supernatant of THP-1 DCs significantly inhibited the proliferation of gastric cancer cells by inducing apoptosis and G1/S cell cycle arrest. Conclusions. Our findings suggest that NVD not only directly inhibits the growth of gastric cancer cells but also exerts indirect antitumor effects by enhancing immune function. These results provide an important theoretical basis for the clinical application of Nidus Vespae in gastric cancer treatment.
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Orozco Hernández, Luis Alberto, Delfina De Jesús Pérez López, Andrés González Huerta, Omar Franco Mora, Martin Rubí Arriaga, and Luis Isaac Aguilera Gómez. "Efecto de Glomus fasciculatum y su relación con tres abonos orgánicos en dos cultivares de haba." Revista Mexicana de Ciencias Agrícolas 7, no. 6 (September 6, 2017): 1423. http://dx.doi.org/10.29312/remexca.v7i6.204.
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El interés de los productores de haba es ponderar entre sustentabilidad y economía; una producción alta y de calidad podría obtenerse con hongos micorrízicos arbusculares (HMA) y abonos orgánicos. El presente trabajo se estableció en el Campo Experimental Rancho “San Lorenzo”, ubicado en el municipio de Metepec, estado de México. El objetivo principal fue evaluar Glomus fasciculatum con lombricomposta, gallinaza y composta de champiñón en 1, 2 y 3 t ha-1 y N60-P60-K30 aplicado en dos cultivares de haba. Los 40 tratamientos se aleatorizaron en un diseño experimental de bloques completos al azar con tres repeticiones, en arreglo de parcelas divididas; ambos cultivares con y sin micorriza (C+M) fueron la parcela grande y el tipo y la dosis de abono la parcela chica (B). La unidad experimental constó de tres surcos de 4 x 0.80 m. La distancia entre plantas fue de 0.40 m. Se detectaron diferencias significativas para ambos factores y en su interacción en 19 de las 21 variables. Al aplicar G. fasciculatum el cv. San Pedro (C1) expresó el mayor promedio en NNF, AP, IV, NVP, PVP, PSL, P100 S, RG, %COL, LRz, NRs, NBs, % VES y % ARB. La gallinaza y la composta de champiñón con 3 t ha-1 favorecieron una mejor expresión fenotípica en la mayoría de las variables evaluadas. C1 con 3 t ha-1 de gallinaza y HMA mostró el mejor comportamiento para AP, IV, NVP, PSL, P100S, y RG (2.63 t ha-1). C1 con 3 t ha-1 de champiñón y micorrizas originó mayor % COL (72.20%) y de ARB (71.46 %). El cv. Santiago (C2) con 3 t ha-1 de gallinaza y HMA mostraron el mayor PVP (124.10 g), NSL (32.63), PSL (62.40 g), RG (2.40 t ha-1), % ARB (73.30) e % hifas (84.40). Los componentes principales 1 y 2 explicaron 52.42% de la variación total original.
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de Faria, Luciano C., Marcelo A. Romero, and Lúcia F. S. Pirró. "Evaluation of a Coupled Model to Predict the Impact of Adaptive Behaviour in the Thermal Sensation of Occupants of Naturally Ventilated Buildings in Warm-Humid Regions." Sustainability 13, no. 1 (December 29, 2020): 255. http://dx.doi.org/10.3390/su13010255.
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Improving indoor environment quality and making urban centres in tropical regions more sustainable has become a challenge for which computational models for the prediction of thermal sensation for naturally ventilated buildings (NVBs) have major role to play. This work performed analysis on thermal sensation for non-residential NVBs located in Brazilian tropical warm-humid climate and tested the effectiveness of suggested adaptive behaviours to mitigate warm thermal sensation. The research method utilized transient computational fluid dynamics models coupled with a dynamic model for human thermophysiology to predict thermal sensation. The calculated results were validated with comparison with benchmark values from questionnaires and from field measurements. The calculated results for dynamic thermal sensation (DTS) seven-point scale showed higher agreement with the thermal sensation vote than with the predicted mean vote. The test for the suggested adaptive behaviours considered reducing clothing insulation values from 0.18 to 0.32 clo (reducing DTS from 0.1 to 0.9), increasing the air speed in 0.9 m/s (reducing DTS from 0.1 to 0.9), and applying both suggestions together (reducing DTS from 0.1 to 1.3) for five scenarios with operative temperatures spanning 34.5–24.0 °C. Results quantified the tested adaptive behaviours’ efficiency showing applicability to improve thermal sensation from slightly-warm to neutral.
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Deacon, CF, S. Wamberg, P. Bie, TE Hughes, and JJ Holst. "Preservation of active incretin hormones by inhibition of dipeptidyl peptidase IV suppresses meal-induced incretin secretion in dogs." Journal of Endocrinology 172, no. 2 (February 1, 2002): 355–62. http://dx.doi.org/10.1677/joe.0.1720355.
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The incretin hormones glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are degraded by dipeptidyl peptidase IV (DPP IV), thereby losing insulinotropic activity. DPP IV inhibition reduces exogenous GLP-1 degradation, but the extent of endogenous incretin protection has not been fully assessed, largely because suitable assays which distinguish between intact and degraded peptides have been unavailable. Using newly developed assays for intact GLP-1 and GIP, the effect of DPP IV inhibition on incretin hormone metabolism was examined. Conscious dogs were given NVP-DPP728, a specific DPP IV inhibitor, at a dose that inhibited over 90% of plasma DPP IV for the first 90 min following treatment. Total and intact incretin concentrations increased (P<0.0001) following a mixed meal, but on control days (vehicle infusion), intact peptide concentrations were lower (P<0.01) than total peptide concentrations (22.6 +/- 1.2% intact GIP; 10.1 +/- 0.4% intact GLP-1). Following inhibitor treatment, the proportion of intact peptide increased (92.5 +/- 4.3% intact GIP, P<0.0001; 99.0 +/- 22.6% intact GLP-1, P<0.02). Active (intact) incretins increased after NVP-DPP728 (from 4797 +/- 364 to 10 649 +/- 106 pM x min for GIP, P<0.03; from 646 +/- 134 to 2822 +/- 528 pM x m in for GLP-1, P<0.05). In contrast, total incretins fell (from 21 632 +/- 654 to 12 084 +/- 1723 pM x min for GIP, P<0.002; from 5145 +/- 677 to 3060 +/- 601 pM x min for GLP-1, P<0.05). Plasma glucose, insulin and glucagon concentrations were unaltered by the inhibitor. We have concluded that DPP IV inhibition with NVP-DPP728 prevents N-terminal degradation of endogenous incretins in vivo, resulting in increased plasma concentrations of intact, biologically active GIP and GLP-1. Total incretin secretion was reduced by DPP IV inhibition, suggesting the possibility of a feedback mechanism.
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Yamagishi, Akane, Yuki Ikeda, Masayoshi Ikeuchi, Ryuzaburo Yuki, Youhei Saito, and Yuji Nakayama. "Targeting Insulin-Like Growth Factor 1 Receptor Delays M-Phase Progression and Synergizes with Aurora B Inhibition to Suppress Cell Proliferation." International Journal of Molecular Sciences 21, no. 3 (February 5, 2020): 1058. http://dx.doi.org/10.3390/ijms21031058.
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The insulin-like growth factor 1 receptor (IGF1R) is a receptor-type tyrosine kinase that transduces signals related to cell proliferation, differentiation, and survival. IGF1R expression is often misregulated in tumor cells, but the relevance of this for cancer progression remains unclear. Here, we examined the impact of IGF1R inhibition on cell division. We found that siRNA-mediated knockdown of IGF1R from HeLa S3 cells leads to M-phase delays. Although IGF1R depletion causes partial exclusion of FoxM1 from the nucleus, quantitative real-time PCR revealed that the transcription of M-phase regulators is not affected by decreased levels of IGF1R. Moreover, a similar delay in M phase was observed following 2 h of incubation with the IGF1R inhibitors OSI-906 and NVP-ADW742. These results suggest that the M-phase delay observed in IGF1R-compromised cells is not caused by altered expression of mitotic regulators. Live-cell imaging revealed that both prolonged prometaphase and prolonged metaphase underlie the delay and this can be abrogated by the inhibition of Mps1 with AZ3146, suggesting activation of the Spindle Assembly Checkpoint when IGF1R is inhibited. Furthermore, incubation with the Aurora B inhibitor ZM447439 potentiated the IGF1R inhibitor-induced suppression of cell proliferation, opening up new possibilities for more effective cancer chemotherapy.
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Becker, Angelika, Monika Daseking, and Julia Kerner auch Koerner. "Cognitive Profiles in the WISC-V of Children with ADHD and Specific Learning Disorders." Sustainability 13, no. 17 (September 4, 2021): 9948. http://dx.doi.org/10.3390/su13179948.
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Attention Deficit Hyperactivity Disorder (ADHD) has a high comorbidity with specific learning disorders (SLD). Children with ADHD and children with SLD show specific cognitive deficits. This study aims to examine similarities and differences between cognitive profiles of children with ADHD + SLD, children with SLD only, and a control group to find out whether specific or shared deficits can be identified for the groups. We compared the WISC-V profiles of 62 children with ADHD and SLD (19 girls, M-age = 10.44; SD = 2.44), 35 children with SLD (13 girls, M-age = 10.21; SD = 2.11) and 62 control children without ADHD or SLD (19 girls, M-age = 10.42; SD = 2.39). The ADHD + SLD group performed worse than the control group in the WISC-V indices WMI, PSI, FSIQ, AWMI, CPI and worse than the SLD group in these indices and the VCI, NVI and GAI. Therefore, compared to children with SLD, children with ADHD + SLD did not show specific impairments in any particular cognitive domain but rather non-specific impairment in almost all indices. Hence, the WISC-V is suited to depict the cognitive strength and weaknesses of an individual child as a basis for targeted intervention.
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Boyes, E. D. "Low Beam Energies for EDS Chemical Microanalysis in the SEM (LVEDS)." Microscopy and Microanalysis 4, S2 (July 1998): 188–89. http://dx.doi.org/10.1017/s1431927600021061.
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Microanalysis data depe nd strongly on the electron beam energy used in the S E M and the effects are more pronou nced with bulk samples than with thin sections. The situation can be used to advantage but there are also significant problems. The data and any quantitative corrections can be quite different at different beam energies. Th e spectra look different and this can be both helpful and confusing, with low ene rgy x-ra y lines generally more prominent at lower voltages. S p ec tra at several different voltages are particularly informative, both for quantitative analysis and to deco nvo lute three dimensional geometries. There may be no single perfect voltage for an analysis.
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Lovera, Lenon Henrique, Rafael Montanari, Elizeu de Souza Lima, Alan Rodrigo Panosso, Mariele Squizato, and Ingrid Nehmi de Oliveira. "ATRIBUTOS DE UM LATOSSOLO E COMPONENTES PRODUTIVOS DA SOJA: UMA ABORDAGEM LINEAR E GEOESTATÍSTICA." Revista em Agronegócio e Meio Ambiente 11, no. 4 (December 28, 2018): 1109. http://dx.doi.org/10.17765/2176-9168.2018v11n4p1109-1131.
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O estudo dos atributos do solo e componentes de produção da planta utilizando a geoestatística, e a consequente geração de mapas de krigagem, podem ser utilizadas na otimização de fertilizantes e, assim, reduzir a produção. O objetivo deste experimento foi avaliar a dependência espacial dos atributos resistência a penetração (RP), teor de água (TA), densidade do solo (DS), porosidade total (PT), Areia (Are), Argila (Arg), Silte (Sil), fósforo (P), matéria orgânica (MO), potencial Hidrogeniônico (pH), potássio (K) e saturação por bases (V) de um Latossolo Vermelho Distroférrico em duas profundidades (0,00-0,10 m e 0,10-0,20 m), com os componentes produtivos número de vagens por planta (NVP), número de grãos por vagem (NGV), número de grãos por planta (NGP), massa de 100 grãos (MCG) e produtividade de grãos (PG) da soja (Glycine max L.) cultivada em plantio direto no ano agrícola de 2013/2014, no município de Selvíria (MS). Foi instalada uma rede amostral, para a coleta de dados do solo e das plantas, com 100 pontos amostrais distribuídos aletoriamente, em uma área de 7.980 m2. As maiores variabilidades dos atributos analisadas pelo coeficiente de variação ocorreram na camada de 0,00-0,10 m do solo. Alguns dos atributos de solo e planta pesquisados apresentaram dependência espacial, sendo possível mapear a área em estudo. Os valores dos alcances recomendados para os atributos pesquisados deverão estar compreendidos entre 9,9 e 91,8 m.
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Kong, Lin, Lei Zhu, Lanqiang Zhang, Hua Bao, and Changhui Rao. "Real-Time Controller Based on FPGA and DSP for Solar Ground Layer Adaptive Optics Prototype System at 1-m NVST." IEEE Photonics Journal 9, no. 2 (April 2017): 1–11. http://dx.doi.org/10.1109/jphot.2017.2662326.
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Astanto, Ichsan, Fatahul Arifin, Yohandri Bow, and Sirajuddin. "Study of Effect Changing the Blade Shape and Lift Angles on Horizontal Wind Turbine." International Journal of Research in Vocational Studies (IJRVOCAS) 2, no. 1 (April 22, 2022): 33–37. http://dx.doi.org/10.53893/ijrvocas.v2i1.92.
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The lack of energy commodities such as coal and petroleum makes the cost of these commodities soar. This event shows how much countries in the world are still very dependent on fossil fuels, and it is fitting that the transition to new renewable energy can be realized soon, low emission and environmentally friendly energy is the hope in the future. Indonesia has a supply of renewable energy sources that are abundant is a great force for this clean energy revolution. The momentum of the presence of the first wind farm in Indonesia operating in Mattirotasi Village, Sidrap Regency, Central Sulawesi in Indonesia with a wind power plant capacity (PLTB) of 75 MW is positive evidence that the government is starting to pay more attention to the development of renewable energy technology in Indonesia. Turbine technology that is able to optimize wind speed in Indonesia must be the answer so that this energy is more useful. In this study, the idea for an experimental analysis of "Study of the Effect of Blade Shape Change and Lift Angle on Horizontal Wind Tubin using Nvis 6009 Experimentation with Solar and Wind Energy props. The Nvis 6009 is a prop used by vocational schools to understand the concept of a DC power plant based on solar and wind energy. To optimize low wind speed, on Nvis 6009 horizontal axis wind turbine (HAWT) with different type 3 Blade and changes in lift angle, so that we can know the effect of the resulting voltage change, so that we can know the effect of the resulting voltage change. Simple illustrations using the Solidworks application are done as validation of the results of previous research and to obtain preliminary data of research experiments. The final results of the test on Type Blade II obtained maximum data at a Blade angle of 13 ° with a wind speed of 3.9 m / s, Blade Rotation 2587 Rpm produces a voltage of 2.8 V.