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Journal articles on the topic 'Lyoprotectants'

Author: Grafiati

Published: 28 June 2021

Last updated: 10 February 2022

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1

Li, Li, Xu Yan Zong, Li Xiong, Jing Zhang, and Kai Chen. "Optimization of Protection Condition for Composite Freeze-Dried Starter of Paocai." Advanced Materials Research 798-799 (September 2013): 1087–90. http://dx.doi.org/10.4028/www.scientific.net/amr.798-799.1087.

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To obtain the better formula of the biomaterials lyoprotectant for composite starter of Paocai, five lyoprotectants were studied with math method those are orthogonal design, Quadratic polynomial stepwise regression and etc. The results showed, 14.7% of hydrolyzate of skim milk, 1.1% of amount of glucose, 3.4% of amount of glycerol, 5.0% of amount of sodium glutamate, 1.2% of amount of Vc were added to the culture of Lactobacillus. After lyophilized, colonies of freeze-dried starters can reach 3.25×1010 cfu/mL. Response surfaces are drawn according to the mathematical model among factors, and it was discussed the impact of lyoprotectants.

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2

Woo, Joshua, and Jeoung Soo Lee. "Effects of lyoprotectants on long-term stability and transfection efficacy of lyophilized poly(lactide-co-glycolide)-graft-polyethylenimine/plasmid DNA polyplexes." Nanomedicine 16, no. 15 (June 2021): 1269–80. http://dx.doi.org/10.2217/nnm-2021-0065.

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Aim: We investigated the effect of lyoprotectants on the long-term stability and transfection efficiency of lyophilized (Lyo.) polyplexes prepared from poly(lactide-co-glycolide)-graft-polyethylenimine (PgP) and plasmid DNA encoding green fluorescent protein (pGFP). Materials & methods: Lyo. PgP/pGFP polyplexes prepared with/without lyoprotectants were stored at -20°C over 6 months. Polyplex stability was analyzed by gel electrophoresis and heparin competition assay. Transfection efficiency and cytotoxicity were evaluated in rat glioma (C6) cells in medium containing 10% serum. Results: Lyo. PgP/pGFP polyplexes prepared with 5% sucrose as a lyoprotectant remained stable up to 6 months and retained transfection efficiency up to 4 months. Conclusion: Lyo. PgP-based polyplexes retain bioactivity during extended storage, potentially enabling transport to remote regions and less stable settings, increasing access to life-changing gene therapy.

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3

Songyang, Lin, Kang Qiaozhen, Pan Dan, Liu Xin, Lu Laizheng, Hao Limin, and Lu Jike. "Identification of Lyoprotectants Released from Gradient-freezing Pretreated Entocyte in Lactococcus Lactis Subsp. Lactis IL1403." Current Topics in Nutraceutical Research 17, no. 2 (July 29, 2017): 122–34. http://dx.doi.org/10.37290/ctnr2641-452x.17:122-134.

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Performance of Lactococcus lactis as a starter culture in food production largely depends on the use of lyoprotectants during lyophilization. Gradient-freezing of bacterial cultures was conducted at 4°C, −20°C, and −80°C by storing the samples at each temperature for 2 h, successively. The entocytes extracted from the frozen cells were used as a lyoprotectant in the follow-up freeze-drying process of Lactococcus lactis subsp. lactis IL1403. The cell survival rate of gradient-freezing group increased 6.4-fold by bacterial plate count method. Furthermore, a proteomics and bioinformatics method was applied to elucidate the protein changes of Lactococcus lactis subsp. lactis IL1403 in response to gradient-freezing by gel-free proteomics using tandem mass tags (TMTs). The results showed that 121 stress-related proteins were significantly influenced by gradient-freezing. These proteins were involved in several metabolism pathways including ribosome metabolism, amino acid metabolism, quorum sensing, phosphotransferase system (PTS), pentose phosphate pathway, microbial metabolism in diverse environments, and nitrogen metabolism, etc. Some of these proteins especially the up-regulated proteins are potential lyoprotectants in vitro and they still need to be further investigated.

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4

MacFarlane, D. R., J. Pringle, and G. Annat. "Reversible self-polymerizing high Tg lyoprotectants." Cryobiology 45, no. 2 (October 2002): 188–92. http://dx.doi.org/10.1016/s0011-2240(02)00127-x.

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5

Qi, Kangru, He Chen, Hongchang Wan, Man Hu, and Yuxi Wu. "Response Surface Optimization of Lyoprotectant from Amino Acids and Salts for Bifidobacterium Bifidum During Vacuum Freeze-Drying." Acta Universitatis Cibiniensis. Series E: Food Technology 21, no. 2 (December 1, 2017): 3–10. http://dx.doi.org/10.1515/aucft-2017-0009.

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Abstract High quality probiotic powder can lay the foundation for the commercial production of functional dairy products. The freeze-drying method was used for the preservation of microorganisms, having a deleterious effect on the microorganisms viability. In order to reduce the damage to probiotics and to improve the survival rate of probiotics during freeze-drying, the Response Surface Methodology (RSM) was adopted in this research to optimize lyoprotectant composed of amino acids (glycine, arginine) and salts (NaHCO3 and ascorbic acid). Probiotic used was Bifidobacterium bifidum BB01. The regression model (p<0.05) was obtained by Box–Behnken experiment design, indicating this model can evaluate the freeze-drying survival rate of B. bifidum BB01 under different lyoprotectants. The results indicated these concentrations as optimal (in W/V): glycine 4.5%, arginine 5.5%, NaHCO3 0.8% and ascorbic acid 2.3%, respectively. Under these optimal conditions, the survival rate of lyophilized powder of B. bifidum BB01 was significantly increased by 80.9% compared to the control group (6.9±0.62%), the results were agreement with the model prediction value (88.7%).

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6

Kelly, Jessica M., Elizabeth E. Pearce, Douglas R. Martin, and Mark E. Byrne. "Lyoprotectants modify and stabilize self-assembly of polymersomes." Polymer 87 (March 2016): 316–22. http://dx.doi.org/10.1016/j.polymer.2016.02.007.

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7

Hariyadi, Dewi Melani, Tutiek Purwanti, and Destia Wardani. "Stability of Freeze-Dried Ovalbumin-Alginate Microspheres with Different Lyoprotectants." Research Journal of Pharmacy and Technology 9, no. 1 (2016): 20. http://dx.doi.org/10.5958/0974-360x.2016.00005.6.

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8

Yun, Gyiae, Iqra Haleem, Hyeongmin Kim, Saemi Yoon, Ki‐Hwan Park, and Jaehwi Lee. "Redispersible Freeze‐dried Quercetin‐loaded Liposomal Formulations Stabilized with Lyoprotectants." Bulletin of the Korean Chemical Society 40, no. 6 (June 2019): 594–97. http://dx.doi.org/10.1002/bkcs.11717.

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9

Debulis, Katherine, and Alexander M. Klibanov. "Dramatic enhancement of enzymatic activity in organic solvents by lyoprotectants." Biotechnology and Bioengineering 41, no. 5 (March 5, 1993): 566–71. http://dx.doi.org/10.1002/bit.260410509.

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10

D'Andrea, G., M. L. Salucci, and L. Avigliano. "Effect of lyoprotectants on ascorbate oxidase activity after freeze-drying and storage." Process Biochemistry 31, no. 2 (January 1996): 173–78. http://dx.doi.org/10.1016/0032-9592(95)00045-3.

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11

Li, Jianying, Meina Hu, Huan Xu, Xiu Yu, Feifei Ye, Kaiqian Wang, Xiaojiao Luan, Ling Li, and Di Zhang. "Influence of type and proportion of lyoprotectants on lyophilized ginsenoside Rg3 liposomes." Journal of Pharmacy and Pharmacology 68, no. 1 (January 2016): 1–13. http://dx.doi.org/10.1111/jphp.12489.

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12

Rodriguez Furlán, Laura T., Pamela Aldrete Herrera, Antonio Pérez Padilla, Rosa I. Ortiz Basurto, and Mercedes E. Campderrós. "Assessment of agave fructans as lyoprotectants of bovine plasma proteins concentrated by ultrafiltration." Food Research International 56 (February 2014): 146–58. http://dx.doi.org/10.1016/j.foodres.2013.12.014.

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13

Capolongo, Antonio, Antonello A. Barresi, and Giorgio Rovero. "Freeze-drying of lignin peroxidase: influence of lyoprotectants on enzyme activity and stability." Journal of Chemical Technology & Biotechnology 78, no. 1 (January 2003): 56–63. http://dx.doi.org/10.1002/jctb.742.

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14

Xu, Mengjie, Guangming Chen, Cunhai Zhang, and Shaozhi Zhang. "Study on the Unfrozen Water Quantity of Maximally Freeze-Concentrated Solutions for Multicomponent Lyoprotectants." Journal of Pharmaceutical Sciences 106, no. 1 (January 2017): 83–91. http://dx.doi.org/10.1016/j.xphs.2016.05.007.

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15

Mohammadipanah, Fatemeh, Leila Parvizi, Javad Hamedi, and Farzaneh Azizmohseni. "Protective Effects of Cryoprotectants and Lyoprotectants on the Survival of Persipeptide ProducingStreptomyces zagrosensisUTMC 1154." Biopreservation and Biobanking 15, no. 5 (October 2017): 451–56. http://dx.doi.org/10.1089/bio.2017.0046.

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16

Fonte, Pedro, Francisca Araújo, Vítor Seabra, Salette Reis, Marco van de Weert, and Bruno Sarmento. "Co-encapsulation of lyoprotectants improves the stability of protein-loaded PLGA nanoparticles upon lyophilization." International Journal of Pharmaceutics 496, no. 2 (December 2015): 850–62. http://dx.doi.org/10.1016/j.ijpharm.2015.10.032.

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17

Hirsjärvi, Samuli, Leena Peltonen, Laura Kainu, and Jouni Hirvonen. "Freeze-Drying of Low Molecular Weight Poly(L-lactic acid) Nanoparticles: Effect of Cryo- and Lyoprotectants." Journal of Nanoscience and Nanotechnology 6, no. 9 (September 1, 2006): 3110–17. http://dx.doi.org/10.1166/jnn.2006.439.

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The chemical and physical stability of polymeric nanoparticles is poor in aqueous suspensions, and the drying of these particles is often problematic. In the present study, the stability of freeze-dried low molecular weight poly(L-lactic acid) (PLA) nanoparticles was enhanced by adding glucose and/or lactose to the formulation as cryo- and lyoprotectants, respectively. Also the effect of an extra stabilizer, Tween 80, was studied. The best freeze-dried PLA nanoparticle formulations were achieved, when glucose and lactose were added in combination so that the amount of lactose was double the amount of glucose. With this combination the redispersion of high-quality nanoparticles (homogenous particle dispersion with original size and without aggregates) was achieved. The addition of Tween 80 further improved the quality of freeze-dried PLA nanoparticles by facilitating the redispersion of the lyophilized cake into optimal nanoparticles.

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18

Musumeci, T., L. Vicari, C. A. Ventura, M. Gulisano, R. Pignatello, and G. Puglisi. "Lyoprotected Nanosphere Formulations for Paclitaxel Controlled Delivery." Journal of Nanoscience and Nanotechnology 6, no. 9 (September 1, 2006): 3118–25. http://dx.doi.org/10.1166/jnn.2006.452.

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The preparation and technological characterization of nanosphere formulations (NS) containing the anticancer drug paclitaxel (PTX) are reported. Poly(lactide) (PLA) and poly(lactide-co-glycolide) (PLGA) nanospheres (NS) were prepared by a solvent displacement method. They showed a mean particle size in the range 150–300 nm, with a high homogeneity (polydispersity index <0.3). For long term stability, NS require additional procedures, such as freeze-drying. In this study, the effect on NS particle size and surface charge of different lyoprotectants (mono- and disaccharides, polyalcohols, and hydroxypropyl-ß-cyclodextrin) at various concentrations was tested by means of light scattering size analysis. The formulations freeze-dried with the addition of 10% glucose (w/v) showed interesting characteristics after freeze-drying. They were chosen for specific studies on drug encapsulation efficiency, in vitro drug release and biological activity on the human anaplastic thyroid carcinoma cell line 8305C. The PLGA NS, in particular, showed a cell growth inhibitory activity comparable to the free drug.

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19

Spadaro, Davide, Annalisa Alessandra Ciavorella, Jorge Giovanny Lopez-Reyes, Angelo Garibaldi, and Maria Lodovica Gullino. "Effect of culture age, protectants, and initial cell concentration on viability of freeze-dried cells ofMetschnikowia pulcherrima." Canadian Journal of Microbiology 56, no. 10 (October 2010): 809–15. http://dx.doi.org/10.1139/w10-068.

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The effect of freeze-drying using different lyoprotectants at different concentrations on the viability and biocontrol efficacy of Metschnikowia pulcherrima was evaluated. The effects of initial yeast cell concentration and culture age on viability were also considered. Yeast cells grown for 36 h were more resistant to freeze-drying than were 48 h cells. An initial concentration of 108cells·mL–1favoured the highest survival after freeze-drying. When maltose (25%, m/v) was used as protectant, a high cell viability was obtained (64.2%). Cells maintained a high viability after 6 months of storage at 4 °C. The biocontrol efficacy of freeze-dried cells was similar to the activity of fresh cells on ‘Gala’ apples and was slightly lower on ‘Golden Delicious’ apples. After optimizing freeze-drying conditions, the viability of M. pulcherrima cells was similar to that obtained in other studies. The results constitute a first step towards the commercial development of M. pulcherrima as a biocontrol agent.

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20

Wang, Fuli, Chuanfen Pu, Mengyao Liu, Risheng Li, Yue Sun, Wenting Tang, Qingjie Sun, and Qin Tian. "Fabrication and characterization of walnut peptides-loaded proliposomes with three lyoprotectants: Environmental stabilities and antioxidant/antibacterial activities." Food Chemistry 366 (January 2022): 130643. http://dx.doi.org/10.1016/j.foodchem.2021.130643.

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21

Pieters, Sigrid, Thomas De Beer, Julia Christina Kasper, Dorien Boulpaep, Oliwia Waszkiewicz, Mohammad Goodarzi, Christophe Tistaert, et al. "Near-Infrared Spectroscopy for In-Line Monitoring of Protein Unfolding and Its Interactions with Lyoprotectants during Freeze-Drying." Analytical Chemistry 84, no. 2 (December 23, 2011): 947–55. http://dx.doi.org/10.1021/ac2022184.

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22

Zu, Yuangang, Xinyang Yu, Xiuhua Zhao, Weiguo Wang, and Kunlun Wang. "Nanocrystallization of the Pharmaceutically Active Agent Genipin by an Emulsion Solvent Evaporation Method." Journal of Nanomaterials 2014 (2014): 1–13. http://dx.doi.org/10.1155/2014/240950.

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To improve the water solubility and dissolution rate, genipin was nanocrystallized by an emulsion solvent evaporation method, followed by freeze-drying. The optimization condition of nanocrystallization process was carried out by single-factor experiment. The effects of five experimental parameters, such as concentration of surfactants the proportion of water to organic phase, homogenate speed and time, homogenization pressure and times, and the proportion of genipin to lyoprotectants on the mean particle size (MPS) of genipin nanoparticles, were investigated. Under the optimum conditions by single-factor experiments, genipin nanoparticles with an MPS of 59.8 nm were obtained. The genipin nanoparticles were characterized by SEM, FTIR, XRD, DSC, solvent residue, drug purity test, dissolution testing, and bioavailability analysis. The analysis results indicated that the chemical structure of genipin nanoparticles was unchanged, but the crystallinity was reduced. The solubility of genipin nanoparticles was 9.05 times of the raw drug. In addition, the residual amounts of chloroform and ethanol were separately less than the ICH limit for class II, and the oral bioavailability of the genipin nanoparticles powder was 7.99 times of raw genipin. According to the results above, genipin nanoparticles show the potential application value of its oral absorption.

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23

Srisang, Siriwan, and Norased Nasongkla. "Spray coating of foley urinary catheter by chlorhexidine-loadedpoly(ε-caprolactone) nanospheres: effect of lyoprotectants, characteristics, and antibacterial activity evaluation." Pharmaceutical Development and Technology 24, no. 4 (October 22, 2018): 402–9. http://dx.doi.org/10.1080/10837450.2018.1502317.

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24

Beck-Broichsitter, Moritz, Pia Kleimann, Thomas Schmehl, Thomas Betz, Udo Bakowsky, Thomas Kissel, and Werner Seeger. "Impact of lyoprotectants for the stabilization of biodegradable nanoparticles on the performance of air-jet, ultrasonic, and vibrating-mesh nebulizers." European Journal of Pharmaceutics and Biopharmaceutics 82, no. 2 (October 2012): 272–80. http://dx.doi.org/10.1016/j.ejpb.2012.07.004.

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25

Muñoz-Rojas, Jesús, Patricia Bernal, Estrella Duque, Patricia Godoy, Ana Segura, and Juan-Luis Ramos. "Involvement of Cyclopropane Fatty Acids in the Response of Pseudomonas putida KT2440 to Freeze-Drying." Applied and Environmental Microbiology 72, no. 1 (January 2006): 472–77. http://dx.doi.org/10.1128/aem.72.1.472-477.2006.

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ABSTRACT Pseudomonas putida KT2440, a saprophytic soil bacterium that colonizes the plant root, is a suitable microorganism for the removal of pollutants and a stable host for foreign genes used in biotransformation processes. Because of its potential use in agriculture and industry, we investigated the conditions for the optimal preservation of the strain and its derivatives for long-term storage. The highest survival rates were achieved with cells that had reached the stationary phase and which had been subjected to freeze-drying in the presence of disaccharides (trehalose, maltose, and lactose) as lyoprotectants. Using fluorescence polarization techniques, we show that cell membranes of KT2440 were more rigid in the stationary phase than in the exponential phase of growth. This is consistent with the fact that cells grown in the stationary phase exhibited a higher proportion of C17:cyclopropane as a fatty acid than cells in the exponential phase. Mutants for the cfaB gene, which encodes the main C17:cyclopropane synthase, and for the cfaA gene, which encodes a minor C17:cyclopropane synthase, were constructed. These mutants were more sensitive to freeze-drying than wild-type cells, particularly the mutant with a knockout in the cfaB gene that produced less than 2% of the amount of C17:cyclopropane produced by the parental strain.

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26

Asprea, Martina, Francesca Tatini, Vieri Piazzini, Francesca Rossi, Maria Bergonzi, and Anna Bilia. "Stable, Monodisperse, and Highly Cell-Permeating Nanocochleates from Natural Soy Lecithin Liposomes." Pharmaceutics 11, no. 1 (January 16, 2019): 34. http://dx.doi.org/10.3390/pharmaceutics11010034.

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(1) Background: Andrographolide (AN), the main diterpenoid constituent of Andrographis paniculata, has a wide spectrum of biological activities. The aim of this study was the development of nanocochleates (NCs) loaded with AN and based on phosphatidylserine (PS) or phosphatidylcholine (PC), cholesterol and calcium ions in order to overcome AN low water solubility, its instability under alkaline conditions and its rapid metabolism in the intestine. (2) Methods: The AN-loaded NCs (AN–NCs) were physically and chemically characterised. The in vitro gastrointestinal stability and biocompatibility of AN–NCs in J77A.1 macrophage and 3T3 fibroblasts cell lines were also investigated. Finally, the uptake of nanocarriers in macrophage cells was studied. (3) Results: AN–NCs obtained from PC nanoliposomes were suitable nanocarriers in terms of size and homogeneity. They had an extraordinary stability after lyophilisation without the use of lyoprotectants and after storage at room temperature. The encapsulation efficiency was 71%, while approximately 95% of AN was released in PBS after 24 h, with kinetics according to the Hixson–Crowell model. The in vitro gastrointestinal stability and safety of NCs, both in macrophages and 3T3 fibroblasts, were also assessed. Additionally, NCs had extraordinary uptake properties in macrophages. (4) Conclusions: NCs developed in this study could be suitable for both AN oral and parental administration, amplifying its therapeutic value.

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27

Li, B. Q., and S. P. Tian. "Effect of intracellular trehalose in Cryptococcus laurentii and exogenous lyoprotectants on its viability and biocontrol efficacy on Penicillium expansum in apple fruit." Letters in Applied Microbiology 44, no. 4 (April 2007): 437–42. http://dx.doi.org/10.1111/j.1472-765x.2006.02080.x.

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28

Basholli-Salihu, Mimoza, Monika Mueller, Sharareh Salar-Behzadi, Frank M. Unger, and Helmut Viernstein. "Effect of lyoprotectants on β-glucosidase activity and viability of Bifidobacterium infantis after freeze-drying and storage in milk and low pH juices." LWT - Food Science and Technology 57, no. 1 (June 2014): 276–82. http://dx.doi.org/10.1016/j.lwt.2014.01.011.

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29

Jian-guo, ZUO, HUA Ze-zhao, LIU Baolin, ZHOU Guoyan, and XU Yi. "Annealing Properties of Lyoprotectant Solution." Acta Physico-Chimica Sinica 21, no. 10 (2005): 1178–81. http://dx.doi.org/10.3866/pku.whxb20051024.

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30

SHIRAKASHI, Ryo, Randolph REUSS, Alexander SCHULZ, Vladimir L. SUKHORUKOV, and Ulrich ZIMMERMANN. "Evaluation of FEM Electric Field Analysis Using Electric Properties of Giant Unilamellar Vesicle (GUV)/Jurkat Cell Measured by Electrorotation (Spectroscopy: Introduction of Cryo- and Lyoprotectants by Electrofusion of GUV and Living Cell)." Transactions of the Japan Society of Mechanical Engineers Series B 74, no. 743 (2008): 1625–32. http://dx.doi.org/10.1299/kikaib.74.1625.

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31

Shirakashi, Ryo, Randolph Reuss, Alexander Schulz, Vladimir L. Sukhorukov, and Ulrich Zimmermann. "Evaluation of FEM electric field analysis using the electric properties of giant unilamellar vesicles (GUV) and Jurkat cells measured by electrorotation spectroscopy: Introduction of cryo- and lyoprotectants by electrofusion of GUVs and living cells." Heat Transfer-Asian Research 38, no. 3 (May 2009): 168–82. http://dx.doi.org/10.1002/htj.20242.

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32

Iskandarsyah, HerviantiNurfitria Nugrahani, and Harmita. "Stability study of azelaic acid proethosomes with lyoprotectant as stabilizer." Journal of Advanced Pharmaceutical Technology & Research 9, no. 2 (2018): 61. http://dx.doi.org/10.4103/japtr.japtr_252_18.

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33

Chen, He, Shiwei Chen, Chuanna Li, and Guowei Shu. "Response Surface Optimization of Lyoprotectant forLactobacillus bulgaricusDuring Vacuum Freeze-Drying." Preparative Biochemistry and Biotechnology 45, no. 5 (October 7, 2014): 463–75. http://dx.doi.org/10.1080/10826068.2014.923451.

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Rodríguez Furlán, Laura T., Antonio Pérez Padilla, and Mercedes E. Campderrós. "Inulin like lyoprotectant of bovine plasma proteins concentrated by ultrafiltration." Food Research International 43, no. 3 (April 2010): 788–96. http://dx.doi.org/10.1016/j.foodres.2009.11.015.

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35

Hinrichs, W. L. J., N. N. Sanders, S. C. De Smedt, J. Demeester, and H. W. Frijlink. "Inulin is a promising cryo- and lyoprotectant for PEGylated lipoplexes." Journal of Controlled Release 103, no. 2 (March 2005): 465–79. http://dx.doi.org/10.1016/j.jconrel.2004.12.011.

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36

Kim, Moo-Kon, Hyo-Won Kwak, Jeong-Yun Lee, Hae-Sung Yun, Min-Hwa Kim, and Ki-Hoon Lee. "Effect of Lyoprotectant on the Solubility and Structure of Silk Sericin." International Journal of Industrial Entomology 25, no. 1 (September 30, 2012): 133–37. http://dx.doi.org/10.7852/ijie.2012.25.1.133.

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37

Neupane, Yub Raj, Chenyuan Huang, Xiaoyuan Wang, Wei Heng Chng, Gopalakrishnan Venkatesan, Olga Zharkova, Matthias Gerhard Wacker, et al. "Lyophilization Preserves the Intrinsic Cardioprotective Activity of Bioinspired Cell-Derived Nanovesicles." Pharmaceutics 13, no. 7 (July 9, 2021): 1052. http://dx.doi.org/10.3390/pharmaceutics13071052.

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Recently, bioinspired cell-derived nanovesicles (CDNs) have gained much interest in the field of nanomedicine due to the preservation of biomolecular structure characteristics derived from their parent cells, which impart CDNs with unique properties in terms of binding and uptake by target cells and intrinsic biological activities. Although the production of CDNs can be easily and reproducibly achieved with any kind of cell culture, application of CDNs for therapeutic purposes has been greatly hampered by their physical and chemical instability during long-term storage in aqueous dispersion. In the present study, we conceived a lyophilization approach that would preserve critical characteristics regarding stability (vesicles’ size and protein content), structural integrity, and biological activity of CDNs for enabling long-term storage in freeze-dried form. Compared to the lyoprotectant sucrose, trehalose-lyoprotected CDNs showed significantly higher glass transition temperature and lower residual moisture content. As assessed by ATR-FTIR and far-UV circular dichroism, lyophilization in the presence of the lyoprotectant effectively maintained the secondary structure of cellular proteins. After reconstitution, lyoprotected CDNs were efficiently associated with HeLa cells, CT26 cells, and bone marrow-derived macrophages at a rate comparable to the freshly prepared CDNs. In vivo, both lyoprotected and freshly prepared CDNs, for the first time ever reported, targeted the injured heart, and exerted intrinsic cardioprotective effects within 24 h, attributable to the antioxidant capacity of CDNs in a myocardial ischemia/reperfusion injury animal model. Taken together, these results pave the way for further development of CDNs as cell-based therapeutics stabilized by lyophilization that enabled long-term storage while preserving their activity.

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Miao, Zongcheng, Yang Zhao, and Xiaoping Huo. "Assessment and determination of lyoprotectant for survival of freeze-dried Lactobacillus rhamnosus." Acta Universitatis Cibiniensis. Series E: Food Technology 20, no. 1 (June 1, 2016): 105–13. http://dx.doi.org/10.1515/aucft-2016-0009.

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Abstract Currently research of lactic acid bacteria focus primarily on the functional probiotics, which are major beneficial biota in the gastrointestinal tract, have been industrial manufactured. Probiotics confer health benefits on the host need adequate amounts. However, the absence of data makes it difficult to ensure the maintenance biological activities and population of probiotic. In this research, a fractional factorial design and steepest ascent experiment were used to analyze the influence of lyoprotectant as carbohydrates, prebiotics and amino acids on the survival of the probiotic Lactobacillus rhamnosus. The results indicated a maximum survival rate and population of viable bacteria of L. rhamnosus to be 55.84 % and 1.60 ×1011 CFU/g after freeze-dried by using a combination of 10 g/100mL Sucrose, 2.5 g/100mL Isomaltooligosaccharide, 12 g/100mL Hydroxyproline. To a large extent, the survival and viability were dependent on the cryoprotectant used and make probiotics more attractive from a practical application in industrial viewpoint.

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Kavitha, R., and N. Damodharan. "Protective Effect of Various Lyoprotectant on the Survival of Antineoplastic Drug Producing Serratia Marcescens." Indian Journal of Public Health Research & Development 10, no. 4 (2019): 1243. http://dx.doi.org/10.5958/0976-5506.2019.00882.9.

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Hong, Sunhwa, Jun Gyu Sim, and Eun Young Lee. "Effect of Substrates and Lyoprotectant on the Survival Ratio of Lyophilized Bacillus sp. SH1RP8." Microbiology and Biotechnology Letters 43, no. 4 (December 28, 2015): 385–90. http://dx.doi.org/10.4014/mbl.1507.07007.

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Wilding, Kristen M., Emily Long Zhao, Conner C. Earl, and Bradley C. Bundy. "Thermostable lyoprotectant-enhanced cell-free protein synthesis for on-demand endotoxin-free therapeutic production." New Biotechnology 53 (November 2019): 73–80. http://dx.doi.org/10.1016/j.nbt.2019.07.004.

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Kannan, Vinayagam, Pavan Balabathula, Laura A. Thoma, and George C. Wood. "Effect of sucrose as a lyoprotectant on the integrity of paclitaxel-loaded liposomes during lyophilization." Journal of Liposome Research 25, no. 4 (December 23, 2014): 270–78. http://dx.doi.org/10.3109/08982104.2014.992023.

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Lee, Hungyen, Dahai Jiang, and William M. Pardridge. "Lyoprotectant Optimization for the Freeze-Drying of Receptor-Targeted Trojan Horse Liposomes for Plasmid DNA Delivery." Molecular Pharmaceutics 17, no. 6 (April 21, 2020): 2165–74. http://dx.doi.org/10.1021/acs.molpharmaceut.0c00310.

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Lian, Bolin, Yong Li, Xiuhua Zhao, Yuangang Zu, Ying Wang, Yin Zhang, and Yuanyuan Li. "Preparation and Optimization of 10-Hydroxycamptothecin Nanocolloidal Particles Using Antisolvent Method Combined with High Pressure Homogenization." Journal of Chemistry 2017 (2017): 1–10. http://dx.doi.org/10.1155/2017/5752090.

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The aim of this study was to prepare 10-hydroxycamptothecin nanocolloidal particles (HCPTNPs) to increase the solubility of drugs, reduce the toxicity, improve the stability of the drug, and so forth. HCPTNPs was prepared by antisolvent precipitation (AP) method combined with high pressure homogenization (HPH), followed by lyophilization. The main parameters during antisolvent process including volume ratio of dimethyl sulfoxide (DMSO) and H2O and dripping speed were optimized and their effects on mean particle size (MPS) and yield of HCPT primary particles were investigated. In the high pressure homogeneous procedure, types of surfactants, amount of surfactants, and homogenization pressure (HP) were optimized and their influences on MPS, zeta potential (ZP), and morphology were analyzed. The optimum conditions of HCPTNPs were as follows: 0.2 mg/mL HCPT aqueous suspension, 1% of ASS, 1000 bar of HP, and 20 passes. Finally, the HCPTNPs via lyophilization using glucose as lyoprotectant under optimum conditions had an MPS of 179.6 nm and a ZP of 28.79 ± 1.97 mV. The short-term stability of HCPTNPs indicated that the MPS changed in a small range.

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Ward, K. R., G. D. J. Adams, H. O. Alpar, and W. J. Irwin. "Protection of the enzyme l-asparaginase during lyophilisation—a molecular modelling approach to predict required level of lyoprotectant." International Journal of Pharmaceutics 187, no. 2 (October 1999): 153–62. http://dx.doi.org/10.1016/s0378-5173(99)00163-5.

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Nugraheni, Raditya Weka, Dwi Setyawan, and Helmy Yusuf. "Physical Characteristics of Liposomal Formulation Dispersed in HPMC Matrix and Freeze-Dried Using Maltodextrin and Mannitol as Lyoprotectant." Pharmaceutical Sciences 23, no. 4 (December 30, 2017): 285–92. http://dx.doi.org/10.15171/ps.2017.42.

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Paramanandana, Abhimata, Retno Sari, Pawahid Pawahid, Eryka A. Novarinandha, and Widji Soeratri. "Pengaruh Lioprotektant Terhadap Karakteristik Nanopartikel Artesunat-Kitosan Yang Dibuat Dengan Gelasi Ionik-Pengeringan Beku." JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA 3, no. 2 (April 9, 2018): 74. http://dx.doi.org/10.20473/jfiki.v3i22016.74-80.

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Latar belakang: Nanopartikel merupakan partikel dengan rentang ukuran diameter antara 1 - 1000 nm. Nanopartikel Artesunat-Khitosan diformulasikan menjadi nanopartikel menggunakan metode gelasi ionik-pengering beku ditujukan untuk melindungi bahan obat dari peruraian selama proses pembuatan. Untuk memperoleh produk dari pengeringan beku yang baik, optimasi lioprotektan sangat diperlukan. Tujuan: Tujuan dari penelitian ini adalah untuk mempelajari karakteristik fisik nanopartikel Artesunat-Khitosan yang diformulasikan dengan tiga lioprotektan yang berbeda: sukrosa, trihalosa dan maltodekstrin menggunakan metode pengeringan beku. Metode: Pembuatan nanopartikel dilakukan menggunakan gelasi ionik dengan tripolifosfat sebagai crosslinker. Evaluasi nanopartikel kering meliputi: morfologi, sifat fisik, spektrum inframerah dan redispersabilitas. Hasil: Data deferential thermal analysis (DTA) menunjukkan, penambahan lioprotektan menjebak artesunat dalam sistem nanopartikel chitosan, dimana formula tanpa penambahan lioprotektan tidak ditemukan artesunat di dalam matriks chitosan. Pemeriksaan morfologi menggunakan scanning electron microscopy (SEM) menunjukkan nanopartikel dengan 2,5% maltodextrin memiliki permukaan yang halus dan bentuk yang speris. Selain itu, maltodextrin menunjukkan efek perlindungan selama pengeringan beku, laju pengendapan yang lebih lambat dan kemampuan untuk redipersi yang lebih baik dibandingkan dengan sukrosa dan trehalosa. Lioprotektan dengan konsentrasi tinggi menunjukkan redispersibilitas nanopartikel artesunat-chitosan yang lebih baik. Kesimpulan: Berdasarkan hasil penelitian ini, dapat disimpulkan bahwa dengan penambahan lyoprotectant mempengaruhi karakterisitik nanopartikel artesunat-chitosan.

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Tsukamoto, Masashi, Tomoyuki Okuda, Hirokazu Okamoto, Yuriko Higuchi, Shigeru Kawakami, Fumiyoshi Yamashita, and Mitsuru Hashida. "Bovine Serum Albumin as a Lyoprotectant for Preparation of DNA Dry Powder Formulations Using the Spray-Freeze Drying Method." Biological and Pharmaceutical Bulletin 35, no. 7 (2012): 1178–81. http://dx.doi.org/10.1248/bpb.b110731.

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Meyers, Stuart A. "Dry storage of sperm: applications in primates and domestic animals." Reproduction, Fertility and Development 18, no. 2 (2006): 1. http://dx.doi.org/10.1071/rd05116.

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Cryopreservation of spermatozoa, oocytes and embryos, as well as somatic cells or cell lines for cloning from cells, are all options for the long-term storage of unique genotypes and endangered species. Spermatozoal cryopreservation and storage currently require liquid nitrogen or ultralow refrigeration-based methods for long- or short-term storage, which requires routine maintenance and extensive space requirements. The preservation of stem cells also has strict requirements for long-term storage to maintain genetic integrity. Dessicated (lyopreserved) sperm and stem cells will provide an unprecedented type of long-term storage without the need for expensive and burdensome cryogenic conditions. Experiments were conducted to determine an effective intracellular concentration of the lyoprotectant trehalose. High-pressure liquid chromatography studies revealed that trehalose can be incorporated into mature sperm cells as well as spermatogonial stem cells from rhesus monkeys. In addition, using fourier transform infrared spectroscopy, we determined that thermotropic phase transitions for fresh ejaculates from rhesus monkey and stallion sperm occurred at 10–15, 33–37 and 55–59°C. Preliminary studies in our laboratory have indicated that spermatogonial stem cells can be dried to <3 g g−1 water and maintain viability following rehydration. Studies in our laboratory have provided preliminary results suggesting that the desiccated storage of sperm and spermatogonial stem cells may be a viable alternative to conventional cryopreservation.

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Purwanti, Tutiek, Maimuna Syamsuar, Dewi Melani Hariyadi, and Tristiana Erawati. "Characteristics And Release Of Gentamicin Sulphate From Sodium Alginate Microspheres Entrapped In Emulgel." International Journal of Drug Delivery Technology 9, no. 04 (December 25, 2019): 704–10. http://dx.doi.org/10.25258/ijddt.9.4.33.

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Gentamicin sulfate is a broad-spectrum aminoglycoside antibiotic that can be used for primary and secondary infections of the skin. Microspheres can be used to extend drug release on the skin; the resulting therapeutic effect is constant and has a longer duration of action. Therefore it can reduce the frequency of use and increase patient compliance. This study investigated the appropriate profile and release kinetics model of gentamicin sulfate microspheres entrapped on the emulgel base. Gentamicin-alginate microspheres were made by the ionotropic gelation method with aerosolization technique, using 2.5% Na-alginate low viscosity, CaCl2 solution of 1.5M as a crosslinker, maltodextrin as lyoprotectant and were dried using the freeze-drying method. The result of microspheres characterization, gentamicin microsphere was spherical with smooth surface structure and had particle diameter of 3.021 ± 0.017μm. Gentamicin microspheres had moisture content 2.89%, and maximum swelling index was 2625 ± 21.70% was achieved within 5 hours. The drug loading of microspheres was 1.75 ± 0.11%, and entrapment efficiency was 10.96 ± 0.19%. The release evaluation during 720 minutes showed that the amount of gentamicin release from alginate microspheres on emulgel base was 14.857 ± 0.816%, and gentamicin release on emulgel conventional was 49.239 ± 5.954%. The model of release kinetics of gentamicin microspheres on emulgel was Higuchi model that showed the release of the active ingredient through the diffusion process. While the model of release kinetics of gentamicin on emulgel base was first order, that showed the release of active agent depends on remaining concentration.

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