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Journal articles on the topic 'Lyophilisation'

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Author: Grafiati

Published: 4 June 2021

Last updated: 22 June 2024

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1

Komissarov, Alexander V., Dmitry N. Bibikov, Oksana A. Volokh, Sergey A. Badarin, Nataliya V. Sinitsyna, Nataliya I. Kostyleva, and Aleksey K. Nikiforov. "Lyophilisation of inactivated vaccines." PROCEEDINGS OF UNIVERSITIES APPLIED CHEMISTRY AND BIOTECHNOLOGY 3, no. 9 (2019): 403–19. http://dx.doi.org/10.21285/2227-2925-2019-9-3-403-419.

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2

Nyanga, Loveness K., Martinus J. R. Nout, Eddy J. Smid, Teun Boekhout, and Marcel H. Zwietering. "Yeasts preservation: alternatives for lyophilisation." World Journal of Microbiology and Biotechnology 28, no. 11 (July 7, 2012): 3239–44. http://dx.doi.org/10.1007/s11274-012-1118-y.

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3

Wolff, Eric, and Henri Gibert. "Développements technologiques nouveaux en lyophilisation." Journal of Food Engineering 8, no. 2 (January 1988): 91–108. http://dx.doi.org/10.1016/0260-8774(88)90057-x.

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4

Mihelčič, Alenka, Klemen Lisjak, and Andreja Vanzo. "Accelerated Solvent Extraction of Phenols from Lyophilised Ground Grape Skins and Seeds." Beverages 9, no. 1 (January 6, 2023): 4. http://dx.doi.org/10.3390/beverages9010004.

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The efficient extraction of phenols from grapes is an important step for their reliable quantification. The aim was to optimise the lyophilisation process and the extraction of phenols from grape skins and seeds. The phenol extraction yield from lyophilised tissues was investigated with different accelerated solvent extraction (ASE) operating conditions. Skins and seeds were separated from frozen berries and lyophilised without being ground. The weight loss during lyophilisation was followed daily. Phenols were extracted from lyophilised, cryo-ground seeds and skins with ASE at room temperature and 10.3 MPa using 80% aqueous acetone and 60% aqueous methanol. The effects of ASE operational parameters (the number of extraction cycles (ECs) and static time (ST) duration) were investigated. The yield of extracted phenols was evaluated spectrophotometrically by determining total phenolic index at 280 nm (TPI). The weight of skins and seeds significantly dropped after 24 h of lyophilisation and continued to decrease, although not significantly, up until the 9th day. The optimal lyophilisation time was estimated to be 3 days and 5 days for skins and seeds, respectively. The phenol extraction yield was significantly affected after changes of ASE conditions. Based on TPI, the optimal ASE conditions were as follows: (i) lyophilised seeds—eight ECs with 10 min ST using aqueous acetone and then four ECs with 20 min ST using aqueous methanol; (ii) lyophilised skins—eight ECs with 1 min ST using aqueous acetone and then one EC with 20 min ST using aqueous methanol.

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5

Prosapio, Valentina, and Estefania Lopez-Quiroga. "Freeze-Drying Technology in Foods." Foods 9, no. 7 (July 13, 2020): 920. http://dx.doi.org/10.3390/foods9070920.

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6

Béal, C., F. Fonseca, A. Thomas, and Mireca Marin. "Caractérisation et lyophilisation de matrices fromagères." Sciences des Aliments 26, no. 1 (February 28, 2006): 89–102. http://dx.doi.org/10.3166/sda.26.89-102.

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7

Ravnik, J., M. Ramšak, M. Zadravec, B. Kamenik, and M. Hriberšek. "Experimental and stochastic analysis of lyophilisation." European Journal of Pharmaceutics and Biopharmaceutics 159 (February 2021): 108–22. http://dx.doi.org/10.1016/j.ejpb.2020.12.011.

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8

Kurcheva, S. A., A. G. Koshkidko, I. V. Zharnikova, D. V. Rusanova, A. A. Semircheva, O. L. Startseva, E. V. Zhdanova, M. M. Kurnoskina, and I. S. Tyumentseva. "Development of a protective lyophilisation medium and conditions to stabilise the erythrocyte diagnostic preparation of tularaemia immunoglobulin." Biological Products. Prevention, Diagnosis, Treatment 22, no. 2 (June 2, 2022): 196–207. http://dx.doi.org/10.30895/2221-996x-2022-22-2-196-207.

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Liquid erythrocyte diagnostic preparations have a practical disadvantage; i.e., long-distance transportation involving possible non-compliance with cold-chain requirements may result in a complete loss of biological activity. A lyophilisation technology is necessary to ensure that the preparations retain their original properties for a long time. The aim of the work was to develop a protective medium and conditions for lyophilisation to stabilise the erythrocyte diagnostic preparation of tularaemia immunoglobulin. Materials and methods: Gelatin, thiourea, trehalose, sucrose, dextran, and Tween 80 were used as excipients for protective media. The authors used nine strains of homologous and heterologous microorganisms of different genera and species to control the lyophilised diagnostic preparation sensitivity and specificity. Evaluation of the main stability-related quality attributes (appearance of the dried preparation, loss on drying, solubility, appearance after reconstitution, appearance after settling, sensitivity, specificity) considered the temperatures specific to the climatic zones where the in vitro diagnostics is intended to be marketed and used. Results: The authors developed protective stabilising media with different compositions, used them in freeze-drying of the preparation and carried out control testing. The most promising was the lyophilisation medium containing a smaller amount of ingredients —6% of dextran, 0.06% of Tween 80 and up to 0.01% of sodium azide—as it was the simplest one to prepare and ensured complete preservation of the quality attributes. The authors carried out practical evaluation of lyophilisation procedures, and the 12–14-hour procedure proved to be the most cost-effective. Conclusions: The results of long-term, or real time, and accelerated stability testing of the lyophilised diagnostic preparation demonstrated the possibility of two-year storage at a labelled temperature of 2–8 °C, as well as at elevated and low temperatures of 30±2 °С and –18 °С, respectively. The tests showed no negative effects of the temperatures on the controlled quality attributes.

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9

Radwan-Pragłowska, J., M. Piątkowski, Ł. Janus, D. Bogdał, and D. Matysek. "Biodegradable, pH-responsive chitosan aerogels for biomedical applications." RSC Advances 7, no. 52 (2017): 32960–65. http://dx.doi.org/10.1039/c6ra27474a.

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10

Palazzese, L., D. A. Anzalone, P. Toschi, and P. Loi. "40 Comparison of slow and rapid freezing in freeze-dried ram spermatozoa." Reproduction, Fertility and Development 32, no. 2 (2020): 146. http://dx.doi.org/10.1071/rdv32n2ab40.

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Semen lyophilisation is an interesting technique that might be a cheap alternative to long-term storage in liquid nitrogen. The first significant result of this method was achieved by Wakayama and Yanagimachi in the 1998 and demonstrated, for the first time, the birth of healthy mouse pups from epididymal freeze-dried (mouse) spermatozoa. The authors follow the lyophilisation technique, commonly used in the pharmaceutical and food industries, namely, deep freezing, which requires direct immersion of the semen sample into liquid nitrogen before vacuum drying. In this work, we focused on the freezing phase to improve and make the technique more reliable. We compared two protocols: 1) rapid freezing, where the semen is plunged directly into liquid nitrogen (LN group), and 2) slow freezing, where the sample is frozen with a freezing rate of 1°C min−1 until −50°C (SL group). Then, both frozen samples were lyophilized. Subsequently, after an interval ranging between 1 and 3 months, dry spermatozoa from LN and SL groups were used for intracytoplasmic sperm injection (ICSI), and the embryo development was evaluated at 24h (2-cell stage) and 7 days (expanded blastocyst) post-ICSI. Moreover, acrosome integrity was evaluated with Pisum sativum agglutinin (PSA) staining on part of the semen, immediately after freezing. The LN-group semen showed the acrosome completely melted, whereas the SL group showed better integrity of the acrosome, which was comparable to that of the normal frozen (vital) spermatozoa. At 24h post-ICSI the number of cleaved embryos in the SL group was higher than in the LN group (42/100 (42%) vs. 19/75 (25.3%), SL and LN, respectively; P=0.0253). The blastocyst rate 7 days after ICSI in the SL group was higher (7/100 (7%) than that in the LN group (2/75 (2.7%); P=0.0238). Our data show that lyophilisation can be conveniently achieved in ram spermatozoa without liquid nitrogen, thus simplifying the procedure. These data support the idea that lyophilisation might be a valuable and cheaper alternative to liquid nitrogen for long-term storage of ram semen.

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11

Safaryan, Abeer H. M., Adam M. Smith, Thomas S. Bedwell, Elena V. Piletska, Francesco Canfarotta, and Sergey A. Piletsky. "Optimisation of the preservation conditions for molecularly imprinted polymer nanoparticles specific for trypsin." Nanoscale Advances 1, no. 9 (2019): 3709–14. http://dx.doi.org/10.1039/c9na00327d.

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Molecularly imprinted polymer nanoparticles are shown as stable after lyophilisation, autoclaving and other common sterilisation techniques, which further extends their shelf-life and is paramount for their application in Life Sciences.

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12

Egashira, Naomichi, Kazuya Yamamoto, and Jun-ichi Kadokawa. "Enzymatic grafting of amylose on chitin nanofibers for hierarchical construction of controlled microstructures." Polymer Chemistry 8, no. 21 (2017): 3279–85. http://dx.doi.org/10.1039/c7py00521k.

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In this study, controlled microstructures were constructed by enzymatic grafting on amidinium chitin nanofibers, followed by lyophilisation, which were changed from network to porous morphologies depending on the molecular weights of amylose graft chains.

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13

Voropaev, A. A., O. V. Fadeikina, T. N. Ermolaeva, and D. S. Davydov. "Lyophilisation of bacterial test strains in a manifold-type apparatus: Effects of freezing and drying parameters, ampoule fill volume, and cotton filter density." Biological Products. Prevention, Diagnosis, Treatment 23, no. 3 (September 8, 2023): 348–60. http://dx.doi.org/10.30895/2221-996x-2023-23-3-348-360.

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Scientific relevance. Lyophilisation is the preferred method at the National Collection of Pathogenic Microorganisms (NCPM) of the Scientific Centre for Expert Evaluation of Medicinal Products of the Ministry of Health of the Russian Federation. Lyophilisation is used to provide for high standards of test-strain deposition, storage, and transportation and to ensure that test strains maintain their properties. Successful lyophilisation requires conducting experiments to establish the key parameters and critical conditions of the process.Aim. The study aimed to evaluate the effects that the speed and time of freezing, the time of drying, the fill volume of ampoules, and the density of cotton filters have on the quality of NCPM indicator microorganisms lyophilised in a manifold-type apparatus.Materials and methods. Pseudomonas aeruginosa NCTC 12924, Staphylococcus aureus NCTC 10788, and Salmonella Abony NCTC 6017 were freeze-dried using a manifold-type apparatus (M. S. R. 18, Usifroid). The authors used a low-temperature freezer at –70±2 °C for slow freezing and a mixture of dry ice and alcohol for quick freezing. The statistical analysis was performed using Microsoft Excel and Statistica 10.Results. The minimum time needed for freezing the samples in a low-temperature freezer at –70±2 °C was 4 hours. Further storage at this temperature for up to 1 month was shown possible without compromising the quality of the final product. The time needed for freezing the samples in a mixture of dry ice and alcohol was under 1 minute. No differences in quality parameters were observed between the lyophilised samples frozen slowly or quickly, except for the cake appearance. Quick freezing resulted in cakes that were non-uniform, crumbled, and pulled away from the ampoule walls, which is considered undesirable. The primary drying stage for ampoules with a fill volume of 0.2 mL took 6–8 hours. The secondary drying stage of 11, 18, 35, and 59 hours resulted in comparable lyophilisate quality: the authors observed no statistically significant differences in viable cell counts (CFU/mL) at the end of lyophilisation and at the end of stress testing. The residual moisture content after 59-hour secondary drying was less than 2%. The cotton filter density had a critical influence on the lyophilisate quality. Therefore, the authors recommend using cotton filters weighing 50 mg or less.Conclusions. The authors analysed the main stages of the lyophilisation process used for NCPM test strains and considered the effects that the speed and time of freezing, the time of drying, the fill volume of ampoules, and the density of cotton filters have on the quality of the final lyophilised product. The NCPM has implemented the results of this study in its work.

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14

Brown, Christopher J., Thomas Simon, Chiara Cilibrasi, Peter J. Lynch, Rhiannon W. Harries, Aline Amorim Graf, Matthew J. Large, et al. "Tuneable synthetic reduced graphene oxide scaffolds elicit high levels of three-dimensional glioblastoma interconnectivity in vitro." Journal of Materials Chemistry B 10, no. 3 (2022): 373–83. http://dx.doi.org/10.1039/d1tb01266e.

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A tuneable 3D scaffold of reduced graphene oxide from a scaleable lyophilisation technique is shown. Good biocompatibility, and a high degree of cellular interconnection in GBM is seen across the scaffold without the use of targeted growth factors.

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15

Bissoyi, Akalabya, Awanish Kumar, Albert A. Rizvanov, Alexander Nesmelov, Oleg Gusev, Pradeep Kumar Patra, and Arindam Bit. "Recent Advances and Future Direction in Lyophilisation and Desiccation of Mesenchymal Stem Cells." Stem Cells International 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/3604203.

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Mesenchymal Stem Cells (MSCs) are a promising mammalian cell type as they can be used for the reconstruction of human tissues and organs. MSCs are shown to form bone, cartilage, fat, and muscle-like cells under specific cultivation conditions. Current technology of MSCs cryopreservation has significant disadvantages. Alternative technologies of mammalian cells preservation through lyophilisation or desiccation (air-drying) are among the upcoming domains of investigation in the field of cryobiology. Different protectants and their combinations were studied in this context. Loading of the protectant in the live cell can be a challenging issue but recent studies have shown encouraging results. This paper deals with a review of the protectants, methods of their delivery, and physical boundary conditions adopted for the desiccation and lyophilisation of mammalian cells, including MSCs. A hybrid technique combining both methods is also proposed as a promising way of MSCs dry preservation.

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16

Tabaszewska, Małgorzata, and Elżbieta Sikora. "The Effect of the Plant Stabilisation Method on the Composition and Antioxidant Properties of Elderflower (Sambucus nigra L.) Extract." Molecules 28, no. 5 (March 4, 2023): 2365. http://dx.doi.org/10.3390/molecules28052365.

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Elderflower extracts are known to be a source of valuable substances that show a wide spectrum of biological activity, including antibacterial and antiviral properties, which demonstrate a degree of effectiveness against SARS CoV-2. In this work, the influence of fresh inflorescence stabilisation methods (freezing, air drying, and lyophilisation) and extraction parameters on the composition and antioxidant properties of the extracts were studied. Wild elderflower plants growing in the Małopolska Region of Poland were studied. Antioxidant activities were evaluated by 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging ability and ferric-reducing antioxidant power assays. The total phenolic content was determined using the Folin-Ciocalteu method and the phytochemical profile of the extracts was analysed using HPLC. The obtained results showed that the best method for the stabilisation of elderflower was lyophilisation, and the determined optimal maceration parameters were 60% methanol as a solvent and a process time of 1–2 days.

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17

Daoud-Mahammed, S., P. Couvreur, and R. Gref. "Novel self-assembling nanogels: Stability and lyophilisation studies." International Journal of Pharmaceutics 332, no. 1-2 (March 2007): 185–91. http://dx.doi.org/10.1016/j.ijpharm.2006.09.052.

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18

Woolfson, A. D., D. F. McCafferty, and A. P. Launchbury. "Stabilisation of hydrotropic temazepam parenteral formulations by lyophilisation." International Journal of Pharmaceutics 34, no. 1-2 (December 1986): 17–22. http://dx.doi.org/10.1016/0378-5173(86)90004-9.

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19

Borůvková, Karolína, and Jakub Wiener. "Properties of modified carboxymethyl cellulose prepared by lyophilisation." Autex Research Journal 13, no. 3 (September 30, 2013): 79–81. http://dx.doi.org/10.2478/v10304-012-0031-7.

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Abstract This study deals with the change of selected properties of samples of modified carboxymethyl cellulose after freeze drying. The investigated properties were changes in thickness, permeability, water sorption, thermal area resistance and thermal absorbing capacity. The prepared materials were evaluated by scanning electron microscopy. The product of presented technology has large internal surface, high wettability and biodegradability. It is nontoxic with high potential in biological applications.

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20

Franks, Felix. "Freeze-Drying/Lyophilisation of Pharmaceutical and Biological Products." Cryobiology 40, no. 4 (June 2000): 381–82. http://dx.doi.org/10.1006/cryo.2000.2256.

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21

Som, S., Subrata Das, S. Dutta, Hendrik G. Visser, Mukesh Kumar Pandey, Pushpendra Kumar, Ritesh Kumar Dubey, and S. K. Sharma. "Synthesis of strong red emitting Y2O3:Eu3+ phosphor by potential chemical routes: comparative investigations on the structural evolutions, photometric properties and Judd–Ofelt analysis." RSC Advances 5, no. 87 (2015): 70887–98. http://dx.doi.org/10.1039/c5ra13247a.

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A comparative investigation on the structural and photoluminescence properties of Y₂O₃:Eu³⁺ phosphor prepared by different routes such as sol-lyophilisation, combustion, hydrothermal and microwave assisted hydrothermal combustion, has been reported for the first time.

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22

Oliver-Urrutia, Carolina, Raúl Rosales Ibañez, Miriam V. Flores-Merino, Lucy Vojtova, Jakub Salplachta, Ladislav Čelko, Jozef Kaiser, and Edgar B. Montufar. "Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells." Materials 14, no. 1 (January 5, 2021): 227. http://dx.doi.org/10.3390/ma14010227.

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This work shows the synthesis of a polyvinylpyrrolidone (PVP) hydrogel by heat-activated polymerization and explores the production of hydrogels with an open porous network by lyophilisation to allow the three-dimensional culture of human oral mucosa stem cells (hOMSCs). The swollen hydrogel showed a storage modulus similar to oral mucosa and elastic solid rheological behaviour without sol transition. A comprehensive characterization of porosity by scanning electron microscopy, mercury intrusion porosimetry and nano-computed tomography (with spatial resolution below 1 μm) showed that lyophilisation resulted in the heterogeneous incorporation of closed oval-like pores in the hydrogel with broad size distribution (5 to 180 μm, d50 = 65 μm). Human oral mucosa biopsies were used to isolate hOMSCs, expressing typical markers of mesenchymal stem cells in more than 95% of the cell population. Direct contact cytotoxicity assay demonstrated that PVP hydrogel have no negative effect on cell metabolic activity, allowing the culture of hOMSCs with normal fusiform morphology. Pore connectivity should be improved in future to allow cell growth in the bulk of the PVP hydrogel.

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23

Mariychuk, R., A. Eliasova, J. Porubska, J. Poracova, and V. Simko. "Isolation and lyophilisation of anthocyanins from fruits of blackcurrant." Acta Horticulturae, no. 1133 (May 2016): 329–34. http://dx.doi.org/10.17660/actahortic.2016.1133.51.

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24

Vayanos, Zoe, Alexander Richardson, Kristie Chapman, and Peter Graham. "Review of lyophilisation of external quality assurance program material." Pathology 54 (March 2022): S83. http://dx.doi.org/10.1016/j.pathol.2021.12.269.

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25

Cao, Xun, Myat Noe Thet, Yu Zhang, Say Chye Joachim Loo, Shlomo Magdassi, Qingyu Yan, and Yi Long. "Solution-based fabrication of VO2 (M) nanoparticles via lyophilisation." RSC Advances 5, no. 33 (2015): 25669–75. http://dx.doi.org/10.1039/c4ra16840b.

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Freeze drying was employed to produce highly pure and crystalline thermochromic VO₂ nanoparticles. By changing the precursor concentration and annealing temperature, particle size and crystallinity can be controlled.

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26

Corveleyn, Sam, Stefaan De Smedt, and Jean Paul Remon. "Moisture absorption and desorption of different rubber lyophilisation closures." International Journal of Pharmaceutics 159, no. 1 (December 1997): 57–65. http://dx.doi.org/10.1016/s0378-5173(97)00263-9.

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27

Paper, D. H., M. Marchesan, and G. Franz. "FC56 hydrolysis of κ and ι-carrageenan by lyophilisation." European Journal of Pharmaceutical Sciences 2, no. 1-2 (September 1994): 114. http://dx.doi.org/10.1016/0928-0987(94)90165-1.

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28

Pereira, P., S. M. Kelly, A. Cooper, H. J. Mardon, P. R. Gellert, and C. F. van der Walle. "Solution formulation and lyophilisation of a recombinant fibronectin fragment." European Journal of Pharmaceutics and Biopharmaceutics 67, no. 2 (September 2007): 309–19. http://dx.doi.org/10.1016/j.ejpb.2007.03.009.

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29

Ngah, Nurul Aida, George J. Dias, Darryl C. Tong, Siti Noor Fazliah Mohd Noor, Jithendra Ratnayake, Paul R. Cooper, and Haizal Mohd Hussaini. "Lyophilised Platelet-Rich Fibrin: Physical and Biological Characterisation." Molecules 26, no. 23 (November 25, 2021): 7131. http://dx.doi.org/10.3390/molecules26237131.

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Background: Platelet-rich fibrin (PRF) has gained popularity in craniofacial surgery, as it provides an excellent reservoir of autologous growth factors (GFs) that are essential for bone regeneration. However, the low elastic modulus, short-term clinical application, poor storage potential and limitations in emergency therapy use restrict its more widespread clinical application. This study fabricates lyophilised PRF (Ly-PRF), evaluates its physical and biological properties, and explores its application for craniofacial tissue engineering purposes. Material and methods: A lyophilisation method was applied, and the outcome was evaluated and compared with traditionally prepared PRF. We investigated how lyophilisation affected PRF’s physical characteristics and biological properties by determining: (1) the physical and morphological architecture of Ly-PRF using SEM, and (2) the kinetic release of PDGF-AB using ELISA. Results: Ly-PRF exhibited a dense and homogeneous interconnected 3D fibrin network. Moreover, clusters of morphologically consistent cells of platelets and leukocytes were apparent within Ly-PRF, along with evidence of PDGF-AB release in accordance with previously reports. Conclusions: The protocol established in this study for Ly-PRF preparation demonstrated versatility, and provides a biomaterial with growth factor release for potential use as a craniofacial bioscaffold.

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30

Medvedevskikh, M. Y., N. L. Vostrikova, A. S. Sergeeva, and V. V. Studenok. "Application of the lyophilization system for preparation of reference materials for composition of nutrition products." Measurement Standards. Reference Materials 17, no. 1 (May 8, 2021): 35–45. http://dx.doi.org/10.20915/2687-0886-2021-17-1-35-45.

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The article presents the application of the lyophilization system for preparation of reference materials (RM) for composition of nutritive products samples. The purpose of the research was the development of lyophilization procedure of RM for composition of poultry meat samples preparation with certified values of mass fraction of moisture, nitrogen (protein) and fat.The poultry meat of two types was used as the primary material for RM for poultry meat composition preparation -1) white meat (chicken breast); 2) red meat (chicken thigh). The procedure included pounding, boiling, freezing and lyophilisation (syn. freeze drying). The researches for homogeneity were implemented with hot air dryer standard system from the State primary standard GET 173-2017. Measurement of nitrogen (protein) mass fraction was performed on State secondary standard GVET176-1-2010. Measurements of fat mass fraction were performed with accordance to State Primary Reference Measurement Procedure.The total time of lyophilization process for samples № 1 and № 2 was 19 and 28 hours, respectively. The total mass loss for sample № 1 was about 63 %, for sample № 2 was about 65 %. The difference in material moisture mass fraction values of materials dried on different trays of freeze dryer was statistically significant, i. e., material was non-homogeneous. To obtain a homogeneous material, an additional homogenization procedure was performed: grinding in a laboratory mill, sieving through a sieve, thorough mixing and conditioning. The certified values of moisture, nitrogen, protein, fat mass fraction for the sample № 1 were accordingly 4,5 %, 14,74 %, 92,1 %, 7,9 %. The same values in the same sequence were 6,3 %, 12,21 %, 76,3 %, 23,8 %.The procedure of lyophilisation was developed for production of reference materials for composition of boiled and freeze-dried poultry meat. The usage of this lyophilisation system allowed to ensure a RM expiration date of six months at ambient temperature of (7±3) °C and relative humidity no more than 60 %. The Reference Materials for composition of boiled and freeze-dried poultry meat was added into Register of certified RMs under № GSO 11276-2019 by the results.

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31

Antal, Tamás, László Sikolya, and Benedek Kerekes. "Evaluation of freezing pre-treatments for the lyophilisation of apple." Acta Universitatis Sapientiae, Agriculture and Environment 5, no. 1 (December 1, 2013): 56–68. http://dx.doi.org/10.2478/ausae-2014-0004.

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Abstract The effect of freezing rate on the quality of dried Jonagold and Idared (Malus domestica Borkh.) was studied. Apple slices underwent various pre-treatments, i.e. freezing in household freezer (freezing speed/rate: 0,5◦C/min), contact plate freezing (2◦C/min) and vacuumfreezing (3◦C/min). The quality of the freeze-dried product was then evaluated in terms of water activity (aw), hardness, color and rehydration. The texture and color experiments were carried out with texture analyser and colorimeter. The aw of apple slices was measured by aw apparatus. It was found that drying time was influenced by freezing rate. The freezing in household freezer (slow freezing rate) significantly reduces the duration of the freeze-drying process and consequently the process costs. The slow freezing rate allows the growth of large ice crystals at the beginning of the freeze-drying process; this fact should consequently lead to larger pores and injured cell walls and thus to shorter freeze-drying time. Quality of the freezing in household freezer product was assessed as higher than the quality of the other freezing pre-treated material. Slow freezing rate resulted softer texture and higher rehydration capacity than that of other pre-treated samples. In all cases, slow freezing speed lead to lower final moisture content, total color difference and water activity. Freeze-dried samples prepared with higher freezing rates (3◦C/min) were the most white in color because small pores, originated by sublimation of small ice crystals formed by fast freezing.

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32

Spengler, A., A. Gross, and H. Kaltwasser. "Successful freeze storage and lyophilisation for preservation of Helicobacter pylori." Journal of Clinical Pathology 45, no. 8 (August 1, 1992): 737. http://dx.doi.org/10.1136/jcp.45.8.737.

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33

Marcotty, T., D. Berkvens, R. K. Besa, B. Losson, T. T. Dolan, M. Madder, G. Chaka, P. Van den Bossche, and J. Brandt. "Lyophilisation and resuscitation of sporozoites of Theileria parva: preliminary experiments." Vaccine 22, no. 2 (December 2003): 213–16. http://dx.doi.org/10.1016/s0264-410x(03)00564-4.

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34

Zhai, S., R. Taylor, R. Sanches, and N. K. H. Slater. "Measurement of lyophilisation primary drying rates by freeze-drying microscopy." Chemical Engineering Science 58, no. 11 (June 2003): 2313–23. http://dx.doi.org/10.1016/s0009-2509(03)00090-3.

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35

Daraoui, N., P. Dufour, H. Hammouri, and A. Hottot. "Model predictive control during the primary drying stage of lyophilisation." Control Engineering Practice 18, no. 5 (May 2010): 483–94. http://dx.doi.org/10.1016/j.conengprac.2010.01.005.

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36

Zhang, Shaozhi. "Simulation of the freezing process in lyophilisation of human platelets." Cryobiology 80 (February 2018): 172. http://dx.doi.org/10.1016/j.cryobiol.2017.10.072.

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Sravya, Maddukuri, Rajamanickam Deveswaran, Srinivasan Bharath, Basappa Veerbadraiah Basavaraj, and Varadharajan Madhavan. "Development of Orodispersible Tablets of Candesartan Cilexetil-β-cyclodextrin Complex." Journal of Pharmaceutics 2013 (September 24, 2013): 1–13. http://dx.doi.org/10.1155/2013/583536.

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The aim of this study was to investigate the use of inclusion complexation technique employing β-cyclodextrin in improving the dissolution profile of candesartan cilexetil, a BCS class-II drug, and to formulate the inclusion complex into orodispersible tablets. The inclusion complexes were formed by physical mixing, kneading, coevaporation, and lyophilisation methods. Inclusion complexes were characterized by FTIR, DSC, XRD, NMR, and mass spectral studies. Inclusion complexes prepared using kneading, and lyophilisation techniques in the molar ratio 1 : 5 with β-cyclodextrin were used for formulating orodispersible tablets by direct compression with different superdisintegrants like croscarmellose sodium, crospovidone, sodium starch glycolate, and low substituted hydroxypropyl cellulose in varying concentrations. The directly compressible powder was evaluated for precompression parameters, and the prepared orodispersible tablets were evaluated for postcompression parameters. Drug-excipient compatibility studies showed no interaction, and characterization proved the formation of inclusion complex. In vitro disintegration time was found to be within 3 minutes, and all the formulations showed complete drug release of 100% within 20 minutes. The optimized formulation was found to be stable after 6 months and showed no significant change in drug content. This work proved β-cyclodextrins to be effective solubilizing agent in improving the solubility of poorly water soluble drugs.

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Rybnikář, Alois, Milan Hejtmánek, and Evžen Weigl. "Survival rate of Trichophyton equinum and T. verrucosum mutants at lyophilisation." Czech Mycology 55, no. 3-4 (December 22, 2003): 273–76. http://dx.doi.org/10.33585/cmy.55312.

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Petrig, Ch. "Stuhlelastase-1: Lyophilisation der Stuhlproben verhindert falsch tiefe Resultate bei Diarrhoe." Praxis 91, no. 23 (2002): 1048. http://dx.doi.org/10.1024/0369-8394.91.23.1048.

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Ward, K. R., H. O. Alpar, G. D. J. Adams, K. N. Atuah, and W. J. Irwin. "Effects of lyophilisation on the formation and stability of stealth liposomes." European Journal of Pharmaceutical Sciences 4 (September 1996): S173. http://dx.doi.org/10.1016/s0928-0987(97)86522-0.

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Smith, G., E. Polygalov, M. S. Arshad, T. Page, J. Taylor, and I. Ermolina. "An impedance-based process analytical technology for monitoring the lyophilisation process." International Journal of Pharmaceutics 449, no. 1-2 (June 2013): 72–83. http://dx.doi.org/10.1016/j.ijpharm.2013.03.060.

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Simić, Lidija, Srećko Stopić, Bernd Friedrich, Matej Zadravec, Žiga Jelen, Rajko Bobovnik, Ivan Anžel, and Rebeka Rudolf. "Synthesis of Complex Concentrated Nanoparticles by Ultrasonic Spray Pyrolysis and Lyophilisation." Metals 12, no. 11 (October 24, 2022): 1802. http://dx.doi.org/10.3390/met12111802.

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The development of new multicomponent nanoparticles is gaining increasing importance due to their specific functional properties, i.e., synthesised new complex concentrated nanoparticles (CCNPs) in the form of powder using ultrasonic spray pyrolysis (USP) and lyophilisation from the initial cast Ag20Pd20Pt20Cu20Ni20 alloy, which was in the function of the material after its catalytic abilities had been exhausted. Hydrometallurgical treatment was used to dissolve the cast alloy, from which the USP precursor was prepared. As a consequence of the incomplete dissolution of the cast alloy and the formation of Pt and Ni complexes, it was found that the complete recycling of the alloy is not possible. A microstructural examination of the synthesised CCNPs showed that round and mostly spherical (not 100%) nanoparticles were formed, with an average diameter of 200 nm. Research has shown that CCNPs belong to the group with medium entropy characteristics. A mechanism for the formation of CCNPs is proposed, based on the thermochemical analysis of element reduction with the help of H2 and based on the mixing enthalpy of binary systems.

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Di Tommaso, Claudia, Caterina Como, Robert Gurny, and Michael Möller. "Investigations on the lyophilisation of MPEG–hexPLA micelle based pharmaceutical formulations." European Journal of Pharmaceutical Sciences 40, no. 1 (April 2010): 38–47. http://dx.doi.org/10.1016/j.ejps.2010.02.006.

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Gaffney, Isabella, Jonathan Brett Sallach, Julie Wilson, Edmund Bergström, and Jane Thomas-Oates. "Metabolomic Approaches to Studying the Response to Drought Stress in Corn (Zea mays) Cobs." Metabolites 11, no. 7 (July 3, 2021): 438. http://dx.doi.org/10.3390/metabo11070438.

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Metabolomics is a technique that allows for the evaluation of the entire extractable chemical profile of a plant, for example, using high-resolution mass spectrometry (HRMS) and can be used to evaluate plant stress responses, such as those due to drought. Metabolomic analysis is dependent upon the efficiency of the extraction protocol. Currently, there are two common extraction procedures widely used in metabolomic experiments, those that extract from plant tissue processed in liquid nitrogen or extraction from lyophilised plant tissues. Here, we evaluated the two using non-targeted metabolomics to show that lyophilisation can stabilise the maize (Zea mays) extractable metabolome, increasing throughput and efficiency of extraction as compared to the more traditional processing in liquid nitrogen. Then, we applied the lyophilisation approach to explore the effect of drought upon the maize metabolome in a non-targeted HRMS metabolomics approach. Metabolomics revealed differences in the mature maize metabolome having undergone three drought conditions imposed at two critical development stages (three-leaf stage and grain-fill stage); moreover, this difference was observed across two tissue types (kernel and inner cob/pith). It was shown that under ideal conditions, the biochemical make-up of the tissue types is different. However, under stress conditions, the stress response dominates the metabolic profile. Drought-related metabolites known from other plant systems have been identified and metabolomics has revealed potential novel drought-stress indicators in our maize system.

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Švarc, Tilen, Srećko Stopić, Žiga Jelen, Matej Zadravec, Bernd Friedrich, and Rebeka Rudolf. "Synthesis of Ni/Y2O3 Nanocomposite through USP and Lyophilisation for Possible Use as Coating." Materials 15, no. 8 (April 13, 2022): 2856. http://dx.doi.org/10.3390/ma15082856.

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The Ni/Y2O3 catalyst showed high catalytic activity. Based on this, the aim of this study was to create Ni/Y2O3 nanocomposites powder with two innovative technologies, Ultrasonic Spray Pyrolysis (USP) and lyophilisation. In the USP process, thermal decomposition of the generated aerosols in an N2/H2 reduction atmosphere caused a complete decomposition of the nickel (II) nitrate to elemental Ni, which became trapped on the formed Y2O3 nanoparticles. The Ni/Y2O3 nanocomposite particles were captured via gas washing in an aqueous solution of polyvinylpyrrolidone (PVP) in collection bottles. PVP was chosen for its ability to stabilise nano-suspensions and as an effective cryoprotectant. Consequently, there was no loss or agglomeration of Ni/Y2O3 nanocomposite material during the lyophilisation process. The Ni/Y2O3 nanocomposite powder was analysed using ICP-MS, SEM-EDX, and XPS, which showed the impact of different precursor concentrations on the final Ni/Y2O3 nanocomposite particle composition. In a final step, highly concentrated Ni/Y2O3 nanocomposite ink (Ni/Y2O3 > 0.140 g/mL) and test coatings from this ink were prepared by applying them on a white matte photo paper sheet. The reflection curve of the prepared Ni/Y2O3 nanocomposite coating showed a local maximum at 440 nm with a value of 39% reflection. Given that Ni is located on the surface of the Ni/Y2O3 nanocomposite in the elemental state and according to the identified properties, tests of the catalytic properties of this coating will be performed in the future.

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Quaak, Susanne G. L., John B. A. G. Haanen, Jos H. Beijnen, and Bastiaan Nuijen. "Naked Plasmid DNA Formulation: Effect of Different Disaccharides on Stability after Lyophilisation." AAPS PharmSciTech 11, no. 1 (March 2010): 344–50. http://dx.doi.org/10.1208/s12249-010-9391-2.

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Koetsier, Sabrina, Lisa Jolly, Graham Jones, Peter Graham, Jill Tate, Trisha Andersen, Ronda Greaves, and Lyn Boscato. "Effect of lyophilisation on measurement of selected endocrine analytes – an RCPAQAP study." Pathology 49 (February 2017): S98. http://dx.doi.org/10.1016/j.pathol.2016.12.278.

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Mohammed, Afzal R., Vincent W. Bramwell, Allan G. A. Coombes, and Yvonne Perrie. "Lyophilisation and sterilisation of liposomal vaccines to produce stable and sterile products." Methods 40, no. 1 (September 2006): 30–38. http://dx.doi.org/10.1016/j.ymeth.2006.05.025.

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Zhou, Kangping, Yanshan Dong, Hao Zheng, Bin Chen, Ruifeng Mao, Li Zhou, and Yefu Wang. "Expression, fermentation, purification and lyophilisation of recombinant Subtilisin QK in Pichia pastoris." Process Biochemistry 54 (March 2017): 1–8. http://dx.doi.org/10.1016/j.procbio.2016.12.028.

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Varga, Santiago, Carmen Diez, Lina Fernández, Jenny Álvarez, Adelino Katchicualula, Carlos Hidalgo, Carolina Tamargo, and Maite Carbajo. "Culture system and long-term storage of culture media in the in vitro production of bovine embryos." Acta Veterinaria Hungarica 59, no. 1 (March 1, 2011): 129–39. http://dx.doi.org/10.1556/avet.59.2011.1.12.

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The optimum culture system for in vitro matured and fertilised oocytes still remains to be clarified. Culture media (CM) for mammalian embryos are routinely prepared fresh for use and preserved under refrigeration during one or two weeks. The purposes of this work were (1) to compare the efficiency of a synthetic oviduct fluid (SOF) with two different bovine serum albumin (BSA) concentrations (3 and 8 g/L) for the in vitro production of bovine blastocysts, (2) to test the effect of timing on adding fetal calf serum (FCS) to the SOF, and (3) to evaluate the effects on bovine embryo development of freezing and lyophilisation as procedures for preserving the SOF. Supplementation of SOF with 3 g/L BSA increased Day-7 blastocyst expansion rates (18.3 ± 1.6 vs. 14.4 ± 0.7; P < 0.05), although no differences in hatching rates were found. Addition of FCS to SOFaa (SOF with amino acids) medium supplemented with sodium citrate (SOFaaci) at 48 and at 72 h post-insemination (PI) allowed obtaining higher Day-6 embryo development rates than when FCS was added at 18 or 96 h PI (Day-6 morulae + blastocyst rate: 30.0 ± 1.1, 40.8 ± 1.1, 43.9 ± 2.3 and 39.3 ± 0.5 for FCS addition at 18, 48, 72 and 96 h, respectively). Hatching rates were significantly improved when serum was added at 72 h PI. Finally, both refrigeration and lyophilisation appeared as useful cryopreservation procedures for SOFaaci, although a significant loss of its ability to support embryo development, compared to the control fresh culture medium, was observed.

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