Relevant bibliographies by topics / Lungs Cytology

Academic literature on the topic 'Lungs Cytology'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Lungs Cytology"

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Musin, M. F., A. F. Yusupova, and A. V. Bondarev. "Intensive diagnosis of destructive lung diseases." Kazan medical journal 69, no. 2 (April 15, 1988): 115–18. http://dx.doi.org/10.17816/kazmj97211.

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We studied diagnostic value of different methods of bronchopulmonary pathology research, identified the optimal number of methods for diagnosing destructive processes in the lungs, analyzed economic parameters of one or another method in order to optimize and efficiently use available equipment in the recognition of destructive processes in the lungs. We examined 240 patients with lung diseases in the pulmonology and thoracic departments. From them we chose 100 patients in whom the diagnosis was verified by postoperative biopsy (48), aspiration biopsy and cytology (52).
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Chowdhury, Mohammad Moinuddin, Mahmud Hassan Arif, Enshad Ekram Ullah, Abdullah Al Mamun, Mirza Nurul Karim, and Rajib Biswas. "A Prospective Observational Study between the Value of Sputum Cytology and FNAC of Bronchial Growth in Diagnosing Lung Cancer at Chattogram Medical College Hospital, Bangladesh." Asian Journal of Medical Sciences 12, no. 9 (September 1, 2021): 55–59. http://dx.doi.org/10.3126/ajms.v12i9.37250.

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Background: Lung disease is viewed as perhaps the most far reaching and deadly malignancies all throughout the planet. The most seasoned and most crucial technique is based on sputum cytology. The last outskirts for getting sufficient material are fine needle yearning cytology (FNAC) of bronchial development. Aims and Objective: To relate the meaning of sputum cytology and fine needle goal cytology of bronchial tissue under CT rules for diagnosing cellular breakdown in the lungs. Materials and Methods: This potential observational investigation was completed by the division of medication in Chattogram Medical College Hospital, Chattogram, Bangladesh. Where data was collected from January 2019 to June 2020. A total of 50 patients with a suspected history, symptoms, and risk profile of having primary lung cancer, as demonstrated by chest radiography and CT scan, were chosen for the research population. Fifty patients with clinical and biochemical verification of suspected. All collected data were coding and input in SPSS-25 for further analysis. Both descriptive and inferential statistics were tested. Results: Among the 50 patients the vast majority of the patients were 51-60 years of age and the biggest number of the (94 %) patients were male. Sputum cytology is 8% touchy which isn’t steady with different examinations and CT guided FNAC is 94% sensitive. Conclusion: A definitive point of picture guided histological or cytological examination is to stay away from unnecessary thoracotomy and accomplish a particular determination with sensible exactness and least results. So, in this examination we found that sputum cytology is 8% delicate which isn’t steady with different investigations and CT guided FNAC is 94% touchy to last histological analysis of lung cancer. The discoveries recommended that CT guided FNAC discovered to be protected, feasible and viable.
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Lloyd, J. K., J. H. Harris, and N. T. Nichols. "STEM/EDS microanalysis of smoker lungs and ferruginous bodies." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 670–71. http://dx.doi.org/10.1017/s0424820100127773.

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Previous studies have used digestion techniques with SEM/EDS to identify foreign material in lungs, These studies have identified elements with inclusions of lung tissue exposed to cigarette smoke, and clinical cases having ferruginous bodies. We have established a protocol to examine fixed lung tissue using x-ray microanalysis with STEM. Our technique allowed both localization and elemental analysis of foreign material in tissue. In the present study lung tissue from cigarette smokers and bronchial washings containing ferruginous bodies were examined.Histological slides of smoker lung tissue were examined to locate dark granular inclusions in cells and the interstitium. Cytology cell block slides of bronchial washings depicting ferruginous bodies were also selected. After locating these granules and bodies, tissues were carefully removed from the paraffin blocks, deparaffinized, and embedded in Spurr. Sections ranging in thickness from 180nm - 500nm were cut with a diamond knife and collected on nylon grids.
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Saleh, Husain A., and John Haapaniemi. "Aspiration Biopsy Cytology of Malignant Hemangiopericytoma Metastatic to the Lungs." Acta Cytologica 41, s1 (1997): 1265–68. http://dx.doi.org/10.1159/000333517.

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Liao, Winston W. P., K. S. Clifford Chao, Tom K. Hei, and Simon Cheng. "Association of IL17-expressing γδ t cells with acute radiation-induced pneumonitis." Journal of Clinical Oncology 30, no. 15_suppl (May 20, 2012): e21097-e21097. http://dx.doi.org/10.1200/jco.2012.30.15_suppl.e21097.

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e21097 Background: Radiation pneumonitis is a substantial cause of morbidity and mortality with thoracic radiation for lung cancer. Little is known about the crucial mechanisms of the inflammatory response. We seek to determine if a key mediator of organ-specific inflammatory disorders and innate immune response, IL-17+ γδ T cells, is associated with radiation pneumonitis. Methods: C3HBe/FeJ mice (7 mice/group) were sham-irradiated as controls or exposed to a single dose of 15 Gy thoracic X-ray to develop pneumonitis. We have previously shown that TGFβ has an immunosuppressive activity in radiation pneumonitis. To potentiate the radiation pneumonitis, one group of mice was administered anti-TGFβ therapy with inhibitory TGFβ mAb (1D11, i.p.10 mg/kg/wk). Bronchoalveolar lavage fluid was assessed for cytology and inflammatory cytokine level. Lung tissues were examined for cell infiltration and histopathological changes. Cell surface marker and intracellular cytokine staining were performed on lymphocytes from the digested lungs by flow cytometry. Results: At 10 weeks post-irradiation, the lungs of the irradiated mice showed substantially more alveolar wall edema and increased infiltration of inflammatory cells compared with sham controls. Pneumonitis-involved lungs contained more IL-17+ γδ T cells (0.85% ± 0.00%) compared with sham controls (0.33% ± 0.02%), p<0.001. Furthermore increased IL-17+ γδ T cells were associated with potentiated radiation pneumonitis with anti-TGFβ therapy. There was a significant increased alveolar inflammation in irradiated mice injected with anti-TGFβ mAb. Anti-TGFβ irradiated lungs also contained significantly more IL-17+ γδ T cells (1.17% ± 0.13%) compared with irradiated controls (0.72% ± 0.13%), p<0.001. There was no increase of other TGFβ-dependent T cell subtypes such as IFNγ+ αβ T cells (Th1), IL-17+ αβ T cells (Th17), CD25+ Foxp3+ Tregs, nor activated macrophages in the potentiated pneumonitis lungs. Conclusions: Our findings implicate a novel role for IL17-expressing γδ T cells in radiation pneumonitis. This study reveals this innate immune response pathway as a potential target for therapeutic intervention in radiation lung injury
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Greimelmaier, Kristina, Thomas Hager, Vasily Moskalenko, Stefan Mueller-Huelsbeck, Henning Feist, Kurt Werner Schmid, Alice Seidel, Danny Jonigk, and Jeremias Wohlschlaeger. "Pulmonary echinococcosis: A rare pseudotumour of the lung." Rare Tumors 13 (January 2021): 203636132110097. http://dx.doi.org/10.1177/20363613211009769.

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Cystic echinococcosis is a widely endemic helminthic disease worldwide but occurs only rarely in Central Europe. Humans are infected as ‘aberrant’ hosts by Echinococcus granulosus and develop cysts in numerous different organs. 20%–30% of the affected individuals develop hydatid disease in the lungs with associated complications including pleuritis, lung abscess and pneumothorax. Radiologically, the pulmonary lesions of cystic echinococcosis occasionally pose difficulties in the differential diagnosis of primary lung carcinoma or metastatic disease and vice versa. Herein we report on a case of pulmonary hydatid disease in a 25-year-old Iraqi male presenting with a cystic lesion of the lung associated with thoracic pain and involuntary weight loss. Despite of its rare occurrence in Central Europe, clinicians, radiologists and pathologists should be aware of this entity and its pulmonary manifestations. During frozen section examination, imprint cytology specimens may facilitate the detection of the pathogens.
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Glück, U., and J. O. Gebbers. "Cytopathology of the Nasal Mucosa in Smokers: A Possible Biomarker of Air Pollution?" American Journal of Rhinology 10, no. 1 (January 1996): 55–58. http://dx.doi.org/10.2500/105065896781795193.

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In our search for an easy, reliable, and inexpensive screening method to assess the toxic effects of air pollution and the attendant cancer risk on the respiratory tract, we investigated to what extent brush cytology of the nasal mucosa satisfies these demands. Using brush cytology, we examined the nasal mucosa of 60 cigarette smokers and compared the cytopathologic findings with those of 60 nonsmokers. All subjects were healthy male office workers with no nasal disorders. Mucosal cells were obtained from the maxillo-turbinal region with a small nylon brush, subsequently processed by Papanicolaou staining on a glass slide, and examined “blinded” by cytopathologists. The cytologic findings were normal in 46 of the nonsmokers, with simple squamous cell metaplasia detectable in the remaining 14 of this group. However, in the group of cigarette smokers, 52 showed unequivocal dysplasia of various degrees, whereas merely eight showed only metaplasia. Dividing the smokers into two groups based on pack/years of cigarette consumption revealed that the severity of dysplasia (mild or moderate) correlated well with the pack/years (P = 0.0001). As yet, no significant relation between smoking habits and the incidence of nasal sinus squamous cell carcinomas has been demonstrated in contrast to the established relationship between smoking and carcinomas of the larynx and lungs. Nevertheless, it is conceivable that cytopathologic changes in the nasal mucosa could act as a biomarker reflecting similar changes in the lower respiratory tract.
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Tydén, Olof, Ulf J. Eriksson, and Christian Berne. "Fetal lung maturation in diabetic pregnancy." Acta Endocrinologica 113, no. 3_Suppl (August 1986): S101—S106. http://dx.doi.org/10.1530/acta.0.111s0101.

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Abstract. The increased incidence of the idiopathic respiratory distress syndrome (IRDS) in infants of diabetic mothers may be explained by preterm delivery and asphyxia but the metabolic derangement per se may also be responsible for the inadequate production of surfactant. Experimental studies of the underlying mechanisms in the lungs of fetuses of pregnant diabetic rats have shown a decreased formation of the two major surfactant phospholipids disaturated phosphatidyl choline and phosphatidyl glycerol. In addition, the activities of key enzymes responsible for the production of these phospholipids are decreased in the fetal lung tissue. Inadequate utilization of pulmonary glycogen for surfactant biosynthesis has also been observed. Furthermore, experimental studies support that other changes than fetal hyperinsulinaemia are needed to produce a state of disturbed surfactant production. In human diabetic pregnancy strict metabolic control allows the fetal lungs to mature in a near-normal fashion. The presence of phosphatidyl glycerol in the amniotic fluid seems to be the best available predictor of lung maturity in diabetic pregnancy, in which both the lecithin/sphingomyelin ratio and amniotic fluid cytology may result in false-positive and false-negative values. The trend towards extension of delivery to term will undoubtedly diminish the need for estimation of fetal lung maturity by amniocentesis. Avoiding preterm delivery and adhering to strict metabolic control of the maternal diabetes would be expected to decrease the neonatal respiratory problems in diabetic pregnancy.
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Munkhdelger, Jijgee, Tomoko Shimooka, Yoshinori Koyama, Sadakatsu Ikeda, Yoshiki Mikami, Junya Fukuoka, Takashi Hori, and Andrey Bychkov. "Basaloid Squamous Cell Carcinoma of the Uterine Cervix: Report of a Case With Molecular Analysis." International Journal of Surgical Pathology 29, no. 7 (April 1, 2021): 770–74. http://dx.doi.org/10.1177/1066896921997132.

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There is a lack of knowledge about molecular alterations in basaloid squamous cell carcinoma (BSCC) of the uterine cervix. A 72-year-old woman with a history of previous subtotal hysterectomy and current vaginal bleeding was referred to our hospital. Initially, adenoid cystic carcinoma (ACC) was diagnosed upon cervical cytology and biopsy. Chest imaging showed multiple metastatic lesions in both lungs. The surgical specimen showed BSCC with diffuse p16 immunoreactivity and negativity for S-100, c-kit, and neuroendocrine markers. There was a focal minor ACC component, which could have explained the previous cytology and biopsy diagnosis. Next-generation sequencing with two different panels showed coexisting PIK3CA mutation and NTRK2 fusion with 10 additional variants of unknown significance ( ATR, DAXX, FAM123B, JAK1, KEL, MLL2, NOTCH2, PALB2, POLD1, POLE). The MYB gene fusions were not identified. The patient received chemotherapy with TRK inhibitor larotrectinib and carboplatin, which caused shrinkage of metastatic lung nodules. This is the first report of cervical BSCC with extensive molecular workup, which detected multiple genetic events, including targetable ones, which are potentially implicated in the development of a tumor. The accumulation of data and further studies on this tumor are necessary to define its diagnostic criteria and its clinical and biological behavior.
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Kumar, Ashok, Anil Kumar Geetha Virupakshappa, and Sushma Kenkare Lokanatha. "CT Imaging of Primary Lung Tumours with CT Guided Fine Needle Aspiration Cytology Correlation among Guwahati, Assam Population." Journal of Evidence Based Medicine and Healthcare 8, no. 31 (August 2, 2021): 2855–64. http://dx.doi.org/10.18410/jebmh/2021/522.

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BACKGROUND The primary lung masses (tumours) are those that originate from the lung tissue. Although most primary pulmonary tumours are carcinomas, a large histological spectrum of benign and malignant tumours of the lung exists. Although chest xray is still considered to be the primary imaging modality of lungs, computed tomography (CT) not only shows the segments that are involved but also the extent of involvement. We wanted to study the sensitivity and specificity of CT in the diagnosis of primary neoplastic lesions of lung, study the CT patterns of different histological variants of bronchogenic carcinoma, and correlate CT findings with CT guided fine needle aspiration and cytology (FNAC) findings. METHODS The present descriptive cross-sectional study was conducted among 34 patients suspected clinically of having lung neoplasms, in Gauhati Medical College and Hospital, Guwahati, Assam from December 2010 to November 2011. RESULTS Considering FNAC / histopathological examination (HPE) as the gold standard, the positive predictive value and false negative value of CT scan for diagnosis of neoplastic lesions of lung were 97 % and 3 % respectively, in our study. Among our study population, mean age with lung tumours was 61 years, highest number of cases was seen in the age group of 51 - 60 years (35 %); Males and females affected were 27 (79.41 %), and 7 in number (20.59 %), respectively. CONCLUSIONS CT is more sensitive in the detection of neoplastic lesions of the lung and associated hilar / mediastinal adenopathy than chest roentgenography. CT has a high efficacy in detecting neoplastic lesions of lung, delineating its lobar and segmental anatomy, thereby helping surgical resection of lung. In this study, CT guided FNAC and cytological findings correlated well with CT diagnosis of primary neoplastic lesions of lung. KEYWORDS Primary Lung Tumour, Contrast Enhanced Computed Tomography (CECT), Fine Needle Aspiration and Cytology (FNAC)
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Dissertations / Theses on the topic "Lungs Cytology"

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Neethling, Greta Sophie. "Automated sputum screening using the BD FocalPointTM Slide Profiler : correlation with transbronchial and transthoracic needle aspirates in a high risk population." Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/86677.

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Thesis (MMed)--Stellenbosch University, 2014.
ENGLISH ABSTRACT: Background: Sputum is a non-invasive, economic investigation whereby bronchogenic carcinoma can be identified. Manual cytological screening is labour intensive, time-consuming and requires a continuous high level of alertness. Automation has recently been successfully introduced in gynaecological cytology. Since sputum samples are similar to cervical smears, the question arises as to whether they are also suitable for automated screening. Objective: This study presented with various objectives: 1) To test automated sputum screening using the BD FocalPoint™ Slide Profiler (FP) and compare with manual sputum screening. 2) To determine the sensitivity and specificity of sputum in identification of bronchogenic carcinoma. 3) To ascertain if any clinical, radiological or bronchoscopy findings would be predictors for bronchogenic carcinoma. 4) To determine the significance of adequacy. Method: Sputum samples were collected prospectively from patients attending the Division of Pulmonology at Tygerberg hospital for a transbronchial fine needle aspiration biopsy (TBNA) or a transthoracic fine needle aspiration biopsy (TTNA) for the period from 2010 to 2012. A pre-bronchoscopy sputum was collected and submitted for processing. Stained slides were put through the FP for automated screening. After slides were qualified, sputum slides were put back in the routine screening pool. Correlation was done using the TBNA/TTNA result as the standard to evaluate the sputum results. Results: 108 sputum samples were included in this study. Of the 84.3% malignant (n=91) and 15.7% benign (n=17) cases confirmed with a diagnostic procedure, sputum cytology had a sensitivity of 38.5% (35/91 malignant cases), and a specificity of 100% (17/17 benign cases). Automated screening had a better sensitivity of 94.3% (33/35 positive sputum cases), while manual screening showed a sensitivity of 74.3% (26/35 positive sputum cases) when compared to the final sputum result. Individual parameters with a significant association with positive sputum included the presence of an endobronchial tumour, partial airway obstruction / stenosis, round mass, spiculated mass (negative association), loss of weight (negative association) and squamous cell carcinoma as the histological subtype. Adequacy was not as significant as hypothesised since 85.3% of true positive sputum, but also 65.5% of false negative sputum, had large numbers of alveolar macrophages present. Conclusion: Sputum cytology remains an important part of the screening programme for bronchogenic carcinoma in the public health sector of South Africa. Results confirm that sputum cytology is very specific, and automated screening improves sensitivity. Automated screening proved to be more time efficient, resulting in 83.1% reduction (p<0.0001) in the screening time spent per case by a cytotechnologist. Results confirm that the quantity of alveolar macrophages is not directly proprtional to pathology representation. Positive sputum results did however improve with sputum adequacy, but had no significant association. Recommendations from this study include adopting automated sputum screening.
AFRIKAANSE OPSOMMING: Agtergrond: Die verkryging van ‘n sputummonster is ‘n nie-indringende, ekonomiese ondersoek waardeur bronguskarsinoom identifiseer kan word. Nie-geoutomatiseerde sitologiese ondersoek is arbeidsintensief, tydrowend en vereis ‘n deurlopende hoë vlak van konsentrasie en fokus. Outomatisering is onlangs suksesvol geïmplementeer in ginekologiese sitologie-ondersoeke. Aangesien sputummonsters soortgelyk aan servikale monsters is, het die vraag ontstaan of sputummonsters ook geskik sou wees vir geoutomatiseerde sifting. Doelwit: Hierdie studie het verskeie doelwitte gehad: 1) Om geoutomatiseerde sifting van sputummonsters te toets deur gebruik te maak van BD Focal Point ™ Slide Profiler (FP), en te vergelyk met nie-geoutomatiseerde sputum sifting. 2) Om die sensitiwiteit en spesifisiteit van sputum in die identifikasie van bronguskarsinoom te bepaal. 3) Om vas te stel of enige kliniese, radiologiese of brongoskopiese bevindings bronguskarsinoom sou kon voorspel. 4) Om die belang van ‘n verteenwoordigende monster te bepaal. Metode: ‘n Prospektiewe studie van die pasiënte wat die Divisie van Pulmonologie by Tygerberg Hospitaal vir transbrongiale nodale aspirasie (TBNA) of ‘n transtorakale aspirasie (TTNA) vanaf Julie 2010 tot Mei 2012 bygewoon het, is gedoen. ‘n Prebrongoskopiese sputum is geneem en gestuur vir prosessering. Die gekleurde skuifies is deur die FP gestuur vir geoutomatiseerde ondersoek. Indien die sputumskuifies gekwalifiseer het vir geoutomatiseerde sifting, is hulle in die groep vir ondersoek ingesluit. ‘n Korrelasiestudie, om die sputumresultate te evalueer, is uitgevoer deur die TBNA/TTNA bevindings as standaard te gebruik. Resultate: Vir hierdie studie is 108 sputummonsters ingesluit. Vanuit die 84.3% maligne (n=91) en 15.7% benigne (n=17) gevalle, bevestig deur ‘n diagnostiese prosedure, het sputumsitologie ‘n sensitiwiteit van 38.5% (35/91 maligne gevalle) en ‘n spesifisiteit van 100.0% (17/17 benigne gevalle), getoon. Geoutomatiseerde sifting het ‘n beter sensitiwiteit met 94.3% (33/35 maligne gevalle), terwyl nie-geoutomatiseerde (ondersoek) ‘n sensitiwiteit van 74.3% (26/35 maligne gevalle) wanneer met die finale resultaat vergelyk, gevind. Individuele parameters met ‘n betekenisvolle assosiasie het die teenwoordigheid van ‘n endobrongiale tumor, gedeeltelike lugwegobstruksie / stenose, ronde massa, ‘n spekuleerde massa (negatiewe assosiasie), gewigsverlies (negatiewe assosiasie) en plaveiselkarsinoom as die histologiese subtipe, ingesluit. Geskiktheid van die monster was nie so betekenisvol as wat in die hipotese gestel is nie: aangesien 85.3% van ware positief gediagnoseerde sputummonsters, maar ook 65.5% van die vals negatiewe sputummonsters, groot hoeveelhede alveolêre makrofae ingesluit het. Gevolgtrekking: Sputumsitologie bly steeds ‘n belangrike deel van die siftingsprogram vir bronguskarsinoom in die openbare gesondheidssektor in Suid-Afrika. Resultate van hierdie studie bevestig dat sputumsitologie baie spesifiek is en dat geoutomatiseerde sifting die sensitiwiteit verbeter. Ge-outomatiseerde sifting het bewys dat dit meer tydsbesparend is, met ‘n 83.1% vermindering (p<0.0001) in die siftingstyd wat deur een sitotegnoloog per geval bestee word. Resultate het bevestig dat die hoeveelheid alveolêre makrofae nie direk proporsioneel verwant is tot die patologie nie. Hoe meer verteenwoordigend die sputummonster was, hoe groter was die kanse om ‘n akkurate positiewe diagnose te maak. Die assosiasie van die geskiktheid van die sputummonster en die positiewe resultate het egter nie ‘n statisties betekenisvolle resultaat getoon nie. Aanbevelings vir hierdie studie sluit in die aanwending van geoutomatiseerde sputumondersoeke.
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Boudria, Asma. "Les variants d'épissage du VEGF-A : leur rôle dans la progression et la réponse aux thérapies anti-angiogéniques des carcinomes pulmonaires." Thesis, Grenoble, 2014. http://www.theses.fr/2014GRENV010/document.

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Le VEGF-A est l'un des facteurs de croissance les plus importants au cours de la néo-angiogénèse tumorale. Ce rôle en a fait une cible de choix pour le développement de thérapies. Ainsi, différents médicaments le ciblant (Bevacizumab, AvastinR) ou ciblant ses voies de signalisation (inhibiteurs des récepteurs VEGFR) sont actuellement utilisés en clinique, notamment dans les adénocarcinomes pulmonaires. Cependant, malgré des résultats initiaux prometteurs, un grand nombre de patients apparaissent d'emblée résistants ou échappent à ces thérapies, voir même développent des tumeurs plus agressives. A ce jour, il n'existe aucun moyen d'identifier les patients susceptibles de répondre à ces thérapies. Récemment, de nouveaux variants d'épissage du VEGF-A issus d'un épissage alternatif différentiel au niveau du dernier exon ont été identifiés, les isoformes VEGFxxxb. A l'inverse des VEGFxxx, ces variants sont anti-angiogéniques. Si les effets paracrines des isoformes du VEGF-A sur les cellules endothéliales ont été bien caractérisés, bien peu d'études se sont penchées sur leurs effets autocrines sur les cellules tumorales qui expriment à leur surface les récepteurs VEGFR1 et VEGFR2. Dans ce contexte, le but principal de notre étude était de déterminer le statut d'expression et les fonctions biologiques de l'isoforme VEGF165b dans les tumeurs primaires et dans des modèles cellulaires dérivés de Carcinomes Pulmonaires Non à Petites Cellules (CBnPCs). Nos résultats identifient des profils d'expression variables du VEGF165b chez les patients atteints de CBnPCs, et montrent que de hauts niveaux intratumoraux de VEGF165b sont associés à un envahissement ganglionnaire. De plus, nos résultats identifient une boucle autocrine au travers de laquelle l'activation des récepteurs VEGFR1/VEGFR2 par le VEGF165b conduit à l'apparition de cellules tumorales présentant un phénotype plus invasif. Finalement, nous montrons que le traitement des cellules tumorales par le Bevacizumab (BVZ) mais aussi par les sels de platine auquel il est associé en clinique augmente l'expression du VEGF165b, la signalisation autocrine qu'il active et conduit à l'apparition de cellules tumorales plus agressives et résistantes à l'apoptose induite par le cisplatine. Ces résultats sont la première démonstration de la capacité du VEGF165b à signaliser sur les cellules tumorales. Dans une seconde étape de notre travail, nous avons étudié le rôle que pourraient jouer les intégrine β1 et β3 dans le maintien de la signalisation induite par le VEGF165b dans les cellules de CBnPCs. Nous montrons que le VEGF165b active l'intégrine β1. Cette activation aboutit à un réarrangement du cytosquelette d'actine en fibres de stress, ce qui pourrait favoriser la migration de ces cellules. De manière intéressante, le BVZ est capable d'induire ce même phénotype par un mécanisme nécessitant l'expression du VEGF165b, de l'intégrine β1, mais aussi de l'intégrine β3. La formation de ces fibres de stress est associée à l'activation de voies de signalisation d'aval mettant en jeu la phosphorylation des protéines FAK et cofiline. De plus, nous mettons en évidence l'existence de complexes entre la neuropiline 2 et l'intégrine β1 dans les cellules de CBnPCs qui seraient susceptibles de participer à l'activation de ces voies. Ainsi, le VEGF165b et le BVZ apparaissent capable de signaliser à travers les intégrines β1 et β3 dans les cellules de CBnPCs, suggérant un rôle de ces intégrines dans la réponse des cellules tumorales aux thérapies anti-angiogéniques. En conclusion, nos résultats identifient un rôle du variant VEGF165b dans la progression des CBnPCs et la réponse aux thérapies anti-angiogéniques et aux chimiothérapies, ce qui suggère que le VEGF165b pourrait être un marqueur réponse au BVZ dans les CBnPCs
VEGF-A is one of the most important factors during tumor neoangiogenesis. This role has made it a prime target for the development of therapies. Thus, various drugs targeting VEGF-A (Bevacizumab, Avastin®) or its signaling pathways (VEGFR inhibitors) are currently used in clinical practice, especially in lung adenocarcinomas treatment. However, despite promising initial results, many patients are refractory or escape these therapies, and sometimes, even develop more aggressive tumors. To date, there is no means to identify patients who are likely to respond these treatments. Recently, new splicing variants of VEGF-A resulting from an alternative splicing at the last exon, were identified and called VEGFxxxb. Unlike VEGFxxx, these variants are antiangiogenic. If paracrine effects of VEGF-A isoforms on endothelial cells have been well characterized, few studies have examined their autocrine effects on tumor cells expressing VEGFR1 and VEGFR2. In this context, the main aim of our study was to determine the expression status and biological functions of VEGF165b in primary tumors and derived cell models of non small cell lung carcinoma (NSCLC). Our results identify variable expression profiles of VEGF165b in NSCLC patients, and show that high levels of intratumoral VEGF165b are associated with lymph node metastases. Furthermore, our results identify an autocrine loop through which the VEGFR1/VEGFR2 activation by VEGF165b leads to the appearance a more invasive phenotype on tumor cells. Finally, our results show that treatment of tumor cells by Bevacizumab (BVZ) but also platinum salts, with which it is associated clinics, increases VEGF165b expression and autocrine signaling, which leads to appearance of tumor cells that are more aggressive and resistant to cisplatin-induced apoptosis. These results are the first evidence of the VEGF165b ability to signalize on tumor cells. In a second stage of our work, we investigated the potential role of β1 and β3 integrins in maintaining VEGF165b signaling in NSCLC cells. We show that VEGF165b activates beta1 integrin. This activation leads to a rearrangement of the actin cytoskeleton in stress fibers, which may promote tumor cell migration. Interestingly, BVZ is able to induce this same phenotype by a mechanism requiring the expression of VEGF165b , beta1 integrin and beta3 integrin. The formation of these stress fibers is associated with the activation of downstream signaling pathways involving proteins phosphorylation of FAK and cofilin. In addition, we highlight the existence of neuropilin-2/β1 integrin complexes in NSCLC cells, which are likely to participate in these pathways activation. Thus, VEGF165b and especially BVZ appear able to signal through beata1 and beta3 integrins NSCLC cells, suggesting a role of these integrins in tumor cells response to antiangiogenic therapies. In conclusion, our results are the first evidence of a role of VEGF165b in NSCLC progression and response to antiangiogenic therapies and chemotherapies; they suggest that VEGF165b could be a marker of response to BVZ in NSCLC
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Maier, Eddie. "Analyse multielementaire par fluorescence x a dispersion d'energie des liquides de lavage broncho-alveolaire chez le sujet sain et pour diverses pathologies respiratoires." Université Louis Pasteur (Strasbourg) (1971-2008), 1987. http://www.theses.fr/1987STR13051.

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Hu, Ming-Hsiu, and 胡銘修. "Application of cytology pleural effusion cell block section analysis on lung adenocarcinoma diagnosis." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/14358156470506742637.

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碩士
中山醫學大學
生化暨生物科技研究所
102
Lung cancer is the main cause of cancer-related death worldwide, lung cancer (also known as carcinoma of the lung) is a malignant lung tumor characterized by uncontrolled cell growth in tissues of the lung. The main primary types are small-cell lung cancer (SCLC), and non-small-cell lung cancer (NSCLC). The most common form is on-small cell lung cancer (NSCLC), accounting for 85 % of all cases. Nearly 40% of lung cancers are adenocarcinoma, which usually originates in peripheral lung tissue. However, among people who never-smokers in their lifetimes, adenocarcinoma is the most common form of lung cancer and Easy to transfer pleural effusion caused. About 10% of people with lung cancer do not have symptoms at diagnosis, Outcomes are generally worse in the developing world. Stage is often advanced at the time of diagnosis. These cancers are incidentally found on routine chest radiography. Lung cancer may be seen on chest radiographs and computed tomography (CT) scans. The diagnosis is confirmed by biopsy which is usually performed by bronchoscopy or CT-guidance, these methods have their own constraints on specimen collection. In this study, a method using a cytology pleural effusion cells made cell block through immunohistochemical staining CK7, CK20, and TTF-1 combination approach, In lung adenocarcinoma compliance with CK7 + / CK20-/TTF-1 + its AUC of 0.8425, represents an excellent discriminant, And more easily and quickly, Let clinicians one more method to detect lung adenocarcinoma cancer.
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Liliane, Nganso Nganpiep. "Characterization of the surface macrophages of the avian lung with observations on a phagocytic respiratory epithelium." Thesis, 2001. http://hdl.handle.net/10539/22750.

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Dissertation submitted to the Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, in fulfillment of the requirements for the degree of Master of Science in Anatomical Sciences.
Due to paucity of free respiratory macrophages (FRMs), compared with mammals, birds have been alleged to be more susceptible to pulmonary infections and affliction. The goal of this study was to question or validate this speculation. Twenty-two mature healthy chickens, 24 domestic ducks and 20 rats were used in various experiments. After pulmonary lavage, FRMs were stained with trypan blue for cell count and with neutral red and trypan blue to assess cell longevity. The cell dynamics was determined by counts made on serial lavages. The morphological attributes of the FRMs and that of the respiratory “phagocytic epithelium” were quantified stereo logically. The rat had a significantly greater number of macrophages and the surface density of the filopodia of the FRMs was higher than that of the birds. The volume density of the vesicular bodies of the macrophages in the three groups of animals was not significantly different. Putative cell flux onto the respiratory surface was observed in the birds and not in the mammal. The surface area of the “phagocytic epithelium” of the birds was very extensive. Compared with mammals, in general, FRMs are fewer in birds. Despite this, the pulmonary defensive status in birds may not necessarily be compromised: functionally, avian FRMs appear to be more efficient. Moreover, their defense function is complimented particularly by the phagocytic activity of the epithelium immediate to the blood-gas barrier, the most vulnerable site of the lung-air sac system.
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Anslinger, Tobias. "Interobserver-Agreement zwischen Pneumologen und dem Zytopathologen, die identische TBNA-Ausstriche bewertet haben." Doctoral thesis, 2019. http://hdl.handle.net/21.11130/00-1735-0000-0005-13FA-C.

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Books on the topic "Lungs Cytology"

1

Diagnostic pulmonary cytology. 2nd ed. Chicago: American Society of Clinical Pathologists Press, 1986.

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Fraire, Armando E. Atlas of neoplastic pulmonary disease: Pathology, cytology, endoscopy, and radiology. New York: Springer, 2010.

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T, Schumacker Paul, ed. Respiratory physiology: Basics and applications. Philadephia: W.B. Saunders Co., 1993.

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1946-, Covell Jamie L., ed. Fine needle aspiration cytology and its clinical applications: Breast & lung. Chicago: American Society of Clinical Pathologists Press, 1985.

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S, Brody Jerome, Center David M, and Tkachuk V. A, eds. Signal transduction in lung cells. New York: Dekker, 1993.

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J, Sterk Peter, and Djukanović Ratko, eds. An atlas of induced sputum: An aid for research and diagnosis. Boca Raton: Parthenon Pub. Group, 2003.

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A colour atlas of sputum cytology: The early diagnosis of lung cancer. London, England: Wolfe Medical Publications, 1988.

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A colour atlas of sputum cytology: The early diagnosis of lung cancer. London: Wolfe Medical, 1988.

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M, Effros Richard, and Chang H. K. 1940-, eds. Fluid and solute transport in the airspaces of the lungs. New York: M. Dekker, 1994.

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Stem cells in the respiratory system. New York: Humana Press, 2010.

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Book chapters on the topic "Lungs Cytology"

1

Hirsch, F. R. "Histopathology, Ultrastructure, and Cytology." In Lung Tumors, 37–53. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-82873-7_5.

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Johnston, William W. "Cytology and Lung Cancer." In Clinical and Experimental Pathology of Lung Cancer, 65–74. Dordrecht: Springer Netherlands, 1986. http://dx.doi.org/10.1007/978-94-009-5036-8_8.

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Zydowicz, Sara, Anjana Yeldandi, and Kirtee Raparia. "Cytology of the Lung." In Cancer Treatment and Research, 59–82. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-38850-7_4.

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VanderLaan, Paul A. "Molecular Diagnostics in Lung Cytology." In Molecular Diagnostics in Cytopathology, 223–47. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-97397-5_11.

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Dennis, Katie, and Fang Fan. "Lung and Respiratory Tract Cytology." In Practical Cytopathology, 95–117. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-24059-2_7.

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Domanski, Henryk A., Nastaran Monsef, and Anna M. Domanski. "Lung." In Atlas of Fine Needle Aspiration Cytology, 219–63. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-76980-6_7.

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Domanski, Henryk A., Nastaran Monsef, and Anna M. Domanski. "Lung." In Atlas of Fine Needle Aspiration Cytology, 161–93. London: Springer London, 2013. http://dx.doi.org/10.1007/978-1-4471-2446-7_6.

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Di Lorito, Alessia, Daniel Stieber, and Fernando C. Schmitt. "Molecular Cytology Applications on the Lung." In Molecular Applications in Cytology, 79–102. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-74942-6_5.

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Li, Qing Kay, and Walid E. Khalbuss. "Lung Cytopathology (Bronchial and Aspiration Cytology)." In Diagnostic Cytopathology Board Review and Self-Assessment, 1–119. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1477-7_1.

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Dey, Pranab. "Lung Lesion with Cough and Haemoptysis." In Fine Needle Aspiration Cytology, 145–50. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-9772-1_21.

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Conference papers on the topic "Lungs Cytology"

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Fiori, Mariana, Carlos Marino Cabral Calvano Filho, Pollyanna Dornelas Pereira, Marco Vinícius Fernandes, and Daniela Omar de Souza. "BREAST CRYPTOCOCCOSIS IN IMMUNOCOMPETENT PATIENTS." In Scientifc papers of XXIII Brazilian Breast Congress - 2021. Mastology, 2021. http://dx.doi.org/10.29289/259453942021v31s1022.

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Introduction: Cryptococcosis is prevalent in immunocompromised individuals. Immunocompetent patients can develop latent infections, the breast being a rare focus of primary disease, with few reports in the literature. Case report: GHMC, female, 27 years old, married, ticketing operator, resident in Valparaíso / GO, Brazil. She denied comorbidities, use of medication, smoking or drinking, as well as contact with caves, farms, farms, wild animals and ingestion of game meat. She reported fever (38°C), left mastalgia associated with hardened erythema with subsequent fistulization and removal of purulent secretion. Upon examination, she was in good general condition, with a palpable nodule of about 6 x 4 cm, in union of the lower quadrants (ULQ) of the left breast (LB), which was regular, soft, felt a little painful on palpation, with increased local temperature and without lymph node enlargement or papillary discharge. The ultrasound of the breasts showed a heterogeneous solid mass, with cystic areas of permeation, in ULQ of LB, of 4.2x2.2 cm, partially defined contours coinciding with a nodular image of 4 cm in the same topography in the mammography. Magnetic resonance imaging showed a nodular, irregular, hypodense image in T1, hyperdense in T2, with parietal enhancement and heterogeneous, progressive internal enhancement, in addition to capturing septa, measuring 6.1x4.0x4.6 cm, suggesting mucinous carcinoma. Core biopsy of the solid part of the lesion and collection of mucinous fluid was performed. Concomitantly, oxacillin was started for seven days. There was no laboratory change during the entire disease period. Fifteen days after the end of the antibiotic use, the lesion became an erythematous lenticular ulcer, with flat edges, of 5.0x4.0 cm, with colloid secretion leaving its bed. Histology showed cryptococcosis, and liquid cytology showed cryptococcus neoformans. During immunosuppression investigation, the patient underwent chest and skull CT scans, serology, tumor markers, ANF (antinuclear factor), rheumatoid factor, C3, C4, lumbar puncture and blood cultures (all excluded any immunosuppressive pathology). The treatment was carried out with Fluconazole 800 mg/day for three months, with a reduction to 300mg/day for another three months. Two months after the start of treatment, the lesion resolved. Cryptococcosis is an invasive mycosis with high morbidity and mortality. It affects immunosuppressed individuals, and is rare in immunocompetent individuals. The main pathogenic species, C. neoformans and C. gatti, are prevalent in tropical and subtropical climates. The main sites affected are the brain and the lungs; other sites are rare. The dosage and duration of breast therapy is unknown, but 2- 3g/day of amphotericin B or 400-800mg/day of fluconazole for 8–12 weeks has achieved therapeutic success in reported cases.
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Bulman, William A., Jonas J. Heymann, Nike Beaubier, Roger Maxfield, Mahesh Mansukhani, and Anjali Saqi. "Molecular Testing Of Lung Adenocarcinoma Using EBUS-TBNA Cytology: Concordance Between Cytology And Histology." In American Thoracic Society 2012 International Conference, May 18-23, 2012 • San Francisco, California. American Thoracic Society, 2012. http://dx.doi.org/10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a2539.

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Forsberg, Daniel, and Nastaran Monsef. "Evaluating Cell Nuclei Segmentation for Use on Whole-Slide Images in Lung Cytology." In 2014 22nd International Conference on Pattern Recognition (ICPR). IEEE, 2014. http://dx.doi.org/10.1109/icpr.2014.582.

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Moitra, Subhabrata, Kanchan Pyasi, Vandana Vincent, Rahul Kodgule, and Sundeep Salvi. "Association between sputum cytology and lung function in patients with asthma and COPD." In Annual Congress 2015. European Respiratory Society, 2015. http://dx.doi.org/10.1183/13993003.congress-2015.pa2266.

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Souza, Matheus Fellipe Nascimento de, Ana Paula Teixeira da Silva, Gabriela Santos Bianchin, Maria Eduarda Angelo de Mendonça Fileti, Raddib Eduardo Noleto da Nóbrega de Oliveira, Rafael Pereira Guimarães, Thábata Emanuelle Martins Nunes, Gustavo da Cunha Ribas, Carla Heloísa Cabral Moro, and Alexandre Luiz Longo. "Progressive disseminated histoplasmosis with pulmonary and central nervous system involvement: a case report." In XIII Congresso Paulista de Neurologia. Zeppelini Editorial e Comunicação, 2021. http://dx.doi.org/10.5327/1516-3180.559.

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Context: Histoplasmosis (Histoplasma capsulatum) is a systemic disease that affects the lung and immune system1. The severity of histoplasmosis is directly related to the individual’s immune response since it is an opportunistic pathogen2. It is one of the most prevalent infections in immunocompromised patients due to the use of tumor necrosis factor-alpha (TNF-alpha) inhibitors, resulting in a mortality rate of 20%. The evolution to CNS occurs in 5-10% of patients with disseminated symptoms3. Case Report: M.G.M, a woman, 67 years, was admitted with bilateral tonicclonic seizure with focal onset in the right upper limb. The patient had hypertension, diabetes and rheumatoid arthritis, and was use ASA, glibenclamide, hydrochlorothiazide, losartan, amlodipine, adalimumab and methotrexate. Complementary exams were performed that showed lesions suggestive of microangiopathy on cranial CT; nodular lesions in the pulmonary right upper lobe and prominent lymph nodes in the hilum and mediastinum on chest CT; CSF with increased cytology (monomorphonuclear predominance), without glucose consumption. The biopsy of lung lesions identified Histoplasma capsulatum, confirming the diagnosis of progressive disseminated histoplasmosis with pulmonary and CNS involvement. Thus, treatment with amphotericin B was started, however, the patient died. Conclusions: Histoplasmosis is the most prevalent invasive fungal infection in users of TNF-alpha inhibitors. In these cases, the disease can be more aggressive and have a rapid evolution, with CNS involvement - which confers a worse prognosis. Early diagnosis, suspension of the immunomodulator and adequate treatment for infection control are required.
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Abe, S., T. Shimoyama, Y. Aida, H. Kishi, M. Sato, T. Kimura, D. Osaka, et al. "Rapid Assessment of Bronchial Brush Cytology Is Useful for the Diagnosis of Lung Cancer." In American Thoracic Society 2009 International Conference, May 15-20, 2009 • San Diego, California. American Thoracic Society, 2009. http://dx.doi.org/10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a5770.

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Brun, Clement, Maxim Sauvage, Cyril Guibert, Blandine Laurent, Georgia Karpathiou, Sophie Bayle, Fabien Forest, et al. "Cytology and chest computed tomography findings of pleural effusion in patients with lung cancer." In Annual Congress 2015. European Respiratory Society, 2015. http://dx.doi.org/10.1183/13993003.congress-2015.pa2503.

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Brun, Clement, Pierre Gay, Michelle Cottier, Georgia Karpathiou, Fabrice-Guy Barral, Jean-Michel Vergnon, and Marios E. Froudarakis. "Cytology, chest computed and positron emission tomography correlations in lung cancer malignant pleural effusion." In ERS International Congress 2018 abstracts. European Respiratory Society, 2018. http://dx.doi.org/10.1183/13993003.congress-2018.pa2867.

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Hirai, Noriko, Sasaki Takaaki, Yoshinori Minami, Masahiro Kitada, Naoyuki Miyokawa, and Yoshinobu Ohsaki. "Abstract 4209: Genotypic agreement between histological specimen and preoperative cytology in small lung adenocarcinoma." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-4209.

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Shepherd, Gillian, Rachel Mercer, Olalla Castro, Rebecca Varatharajah, Andrew Thayanandan, Qiang Lu, Maged Hassan, et al. "Outcomes of cytology positive malignant pleural effusions; do certain malignancies predispose patients to trapped lung?" In ERS International Congress 2019 abstracts. European Respiratory Society, 2019. http://dx.doi.org/10.1183/13993003.congress-2019.oa492.

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Reports on the topic "Lungs Cytology"

1

Saccomanno, G. Early Lung Cancer Detection in Uranium Miners with Abnormal Sputum Cytology. Office of Scientific and Technical Information (OSTI), June 2000. http://dx.doi.org/10.2172/834057.

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Saccomanno, G. Early lung cancer detection in uranium miners with abnormal sputum cytology. Office of Scientific and Technical Information (OSTI), August 1992. http://dx.doi.org/10.2172/7091811.

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Saccomanno, G. Early lung cancer detection in uranium miners with abnormal sputum cytology. Technical progress report, July 31, 1991--July 31, 1992. Office of Scientific and Technical Information (OSTI), August 1992. http://dx.doi.org/10.2172/10131296.

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