Academic literature on the topic 'Ltp protein'
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Journal articles on the topic "Ltp protein"
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Abbas, Abdul-Karim, Agnès Villers, and Laurence Ris. "Temporal phases of long-term potentiation (LTP): myth or fact?" Reviews in the Neurosciences 26, no. 5 (October 1, 2015): 507–46. http://dx.doi.org/10.1515/revneuro-2014-0072.
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AbstractLong-term potentiation (LTP) remains the most widely accepted model for learning and memory. In accordance with this belief, the temporal differentiation of LTP into early and late phases is accepted as reflecting the differentiation of short-term and long-term memory. Moreover, during the past 30 years, protein synthesis inhibitors have been used to separate the early, protein synthesis-independent (E-LTP) phase and the late, protein synthesis-dependent (L-LTP) phase. However, the role of these proteins has not been formally identified. Additionally, several reports failed to show an effect of protein synthesis inhibitors on LTP. In this review, a detailed analysis of extensive behavioral and electrophysiological data reveals that the presumed correspondence of LTP temporal phases to memory phases is neither experimentally nor theoretically consistent. Moreover, an overview of the time courses of E-LTP in hippocampal slices reveals a wide variability ranging from <1 h to more than 5 h. The existence of all these conflictual findings should lead to a new vision of LTP. We believe that the E-LTP vs. L-LTP distinction, established with protein synthesis inhibitor studies, reflects a false dichotomy. We suggest that the duration of LTP and its dependency on protein synthesis are related to the availability of a set of proteins at synapses and not to the de novo synthesis of plasticity-related proteins. This availability is determined by protein turnover kinetics, which is regulated by previous and ongoing electrical activities and by energy store availability.
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Nynca, Joanna, Mariola A. Dietrich, Barbara Bilińska, Małgorzata Kotula-Balak, Tomasz Kiełbasa, Halina Karol, and Andrzej Ciereszko. "Isolation of lipocalin-type protein from rainbow trout seminal plasma and its localisation in the reproductive system." Reproduction, Fertility and Development 23, no. 2 (2011): 381. http://dx.doi.org/10.1071/rd10118.
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The lipocalin protein family is a large and diverse group of small extracellular proteins characterised by their ability to bind hydrophobic molecules. In the present study, we describe the isolation procedure for rainbow trout seminal plasma protein, characterised by a moderate migration rate during polyacrylamide gel electrophoresis, providing information regarding its basic features and immunohistochemical localisation. This protein was identified as a lipocalin-type protein (LTP). The molecular mass of LTP was found to be 18 848 Da and it was found to lack any carbohydrate components. Only a few Salmoniformes contain LTP in their seminal plasma. The abundance of LTP in the Sertoli and Leydig cells of the testes of the rainbow trout, as well as in secretory cells of the efferent duct, suggests that this protein is specific for rainbow trout milt, where it acts as a lipophilic carrier protein. Moreover, the specific localisation of LTP in the flagella of the spermatozoa suggests a role for LTP in sperm motility. Further experiments are necessary to identify the endogenous ligands for LTP in rainbow trout seminal plasma and to characterise the binding properties of this protein.
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Huang, Y. Y., and E. R. Kandel. "Recruitment of long-lasting and protein kinase A-dependent long-term potentiation in the CA1 region of hippocampus requires repeated tetanization." Learning & Memory 1, no. 1 (1994): 74–82. http://dx.doi.org/10.1101/lm.1.1.74.
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To study how the late phase of long-term potentiation (LTP) in hippocampus arises, we examined the resulting LTP for its time course and its dependence on protein synthesis and different second-messenger kinases by applying various conditioning tetani. We find that one high-frequency train (100 Hz) produces a form of LTP that lasts longer than 1 hr but less than 3 hr (the early phase of LTP, or E-LTP). It is blocked by inhibitors of calcium/calmodulin kinase II (Cam kinase II) but is not affected by an inhibitor of cAMP-dependent protein kinase [protein kinase A (PKA) and the protein synthesis inhibitor anisomycin] nor is it occluded by the cAMP activator forskolin. In contrast, when three high-frequency trains are used, the resulting potentiation persists for at least 6-10 hr. The L-LTP induced by three trains differs from the E-LTP in that it requires new protein synthesis, is blocked by an inhibitor of cAMP-dependent protein kinase, and is occluded by forskolin. These results indicate that the two mechanistically distinctive forms of LTP, a transient, early component (E-LTP) and a more enduring form (L-LTP), can be recruited selectively by changing the number of conditioning tetanic trains. Repeated tetani induce a PKA and protein synthesis-dependent late component that adds to the amplitude and duration of the potentiation induced by a single tetanus.
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Soderling, Thomas R., and Victor A. Derkach. "Postsynaptic protein phosphorylation and LTP." Trends in Neurosciences 23, no. 2 (February 2000): 75–80. http://dx.doi.org/10.1016/s0166-2236(99)01490-3.
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Chong, L. D. "STKE: Protein Translation and LTP." Science 295, no. 5555 (January 25, 2002): 589c—589. http://dx.doi.org/10.1126/science.295.5555.589c.
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Yang, Hong-Wei, Xiao-Dong Hu, Hong-Mei Zhang, Wen-Jun Xin, Ming-Tao Li, Tong Zhang, Li-Jun Zhou, and Xian-Guo Liu. "Roles of CaMKII, PKA, and PKC in the Induction and Maintenance of LTP of C-Fiber-Evoked Field Potentials in Rat Spinal Dorsal Horn." Journal of Neurophysiology 91, no. 3 (March 2004): 1122–33. http://dx.doi.org/10.1152/jn.00735.2003.
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Long-term potentiation (LTP) of C-fiber-evoked field potentials in spinal dorsal horn may be relevant to hyperalgesia, an increased response to noxious stimulation. The mechanism underlying this form of synaptic plasticity is, however, still unclear. Considerable evidence has shown that calcium/calmodulin-dependent protein kinase II (CaMKII), protein kinase A (PKA), and protein kinase C (PKC) are important for LTP in hippocampus. In this study, the roles of these three protein kinases in the induction and maintenance of LTP of C-fiber-evoked field potentials were evaluated by application of specific inhibitors of CaMKII (KN-93 and AIP), PKA (Rp-CPT-cAMPS), and PKC (chelerythrine and Gö 6983) at the recording segments before and after LTP induction in urethane-anesthetized Sprague-Dawley rats. We found both KN-93 and AIP, when applied at 30 min prior to tetanic stimulation, completely blocked LTP induction. At 30 min after LTP induction, KN-93 and AIP reversed LTP completely, and at 60 min after LTP induction, they depressed spinal LTP in most rats tested. Three hours after LTP induction, however, KN-93 or AIP did not affect the spinal LTP. Rp-CPT-cAMPS, chelerythrine, and Gö 6983 blocked the spinal LTP when applied at 30 min before tetanic stimulation and reversed LTP completely at 15 min after LTP induction. In contrast, at 30 min after LTP induction, the drugs never affected the spinal LTP. These results suggest that activation of CaMKII, PKA, and PKC may be crucial for the induction and the early-phase but not for the late-phase maintenance of the spinal LTP.
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Rizzi, Angela, Raffaella Chini, Riccardo Inchingolo, Valentina Carusi, Franco Pandolfi, Antonio Gasbarrini, and Eleonora Nucera. "Nickel allergy in lipid transfer protein sensitized patients: Prevalence and clinical features." International Journal of Immunopathology and Pharmacology 34 (January 2020): 205873842097489. http://dx.doi.org/10.1177/2058738420974895.
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Nickel (Ni), the main responsible for allergic contact dermatitis worldwide, is also involved in systemic condition called “Systemic Nickel Sulfate Allergy Syndrome (SNAS).” Likewise, IgE-mediated reactivity to Lipid Transfer Protein (LTP) represents the main cause of primary food allergy in adults of Mediterranean countries. We evaluated the prevalence of SNAS in LTP allergic patients and investigated patients’ clinical features with double sensitization (LTP and Ni). A retrospective, single-center, observational study was conducted performing a complete allergological work-up including: (1) skin prick tests; (2) serum specific IgE for plant food allergens and rPru p3 (LTP); (3) patch test with 5% Ni sulfate in petrolatum. We enrolled 140 LTP allergic patients of which 36 patients (25.7% of sample) showed additional positivity to Ni patch test. Patients with double sensitization were more frequently females and reported fewer cutaneous symptoms. Higher values of sIgE for peach, apple, peanut, walnut, grain, corn, and garlic were found in LTP allergic patients, while higher values for hazelnut in the other subgroup. The prevalence of SNAS in the LTP allergic population is clinically relevant. Moreover, the clinical and immunological profiles of patients with double sensitization were different from patients monosensitized to LTP.
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Sheng, Nengyin, Michael A. Bemben, Javier Díaz-Alonso, Wucheng Tao, Yun Stone Shi, and Roger A. Nicoll. "LTP requires postsynaptic PDZ-domain interactions with glutamate receptor/auxiliary protein complexes." Proceedings of the National Academy of Sciences 115, no. 15 (March 26, 2018): 3948–53. http://dx.doi.org/10.1073/pnas.1800719115.
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Long-term potentiation (LTP) is a persistent strengthening of synaptic transmission in the brain and is arguably the most compelling cellular and molecular model for learning and memory. Previous work found that both AMPA receptors and exogenously expressed kainate receptors are equally capable of expressing LTP, despite their limited homology and their association with distinct auxiliary subunits, indicating that LTP is far more promiscuous than previously thought. What might these two subtypes of glutamate receptor have in common? Using a single-cell molecular replacement strategy, we demonstrate that the AMPA receptor auxiliary subunit TARP γ-8, via its PDZ-binding motif, is indispensable for both basal synaptic transmission and LTP. Remarkably, kainate receptors and their auxiliary subunits Neto proteins share the same requirement of PDZ-binding domains for synaptic trafficking and LTP. Together, these results suggest that a minimal postsynaptic requirement for LTP is the PDZ binding of glutamate receptors/auxiliary subunits to PSD scaffolding proteins.
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Abbas, Abdul-Karim, Mikhail Dozmorov, Rui Li, Fen-Sheng Huang, Fredrik Hellberg, Jonas Danielson, Ye Tian, Jörgen Ekström, Mats Sandberg, and Holger Wigström. "Persistent LTP without triggered protein synthesis." Neuroscience Research 63, no. 1 (January 2009): 59–65. http://dx.doi.org/10.1016/j.neures.2008.10.008.
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Goto, Jun-Ichi, Satoshi Fujii, Hiroki Fujiwara, Katsuhiko Mikoshiba, and Yoshihiko Yamazaki. "Synaptic plasticity in hippocampal CA1 neurons of mice lacking inositol-1,4,5-trisphosphate receptor-binding protein released with IP3 (IRBIT)." Learning & Memory 29, no. 4 (March 29, 2022): 110–19. http://dx.doi.org/10.1101/lm.053542.121.
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In hippocampal CA1 neurons of wild-type mice, a short tetanus (15 or 20 pulses at 100 Hz) or a standard tetanus (100 pulses at 100 Hz) to a naive input pathway induces long-term potentiation (LTP) of the responses. Low-frequency stimulation (LFS; 1000 pulses at 1 Hz) 60 min after the standard tetanus reverses LTP (depotentiation [DP]), while LFS applied 60 min prior to the standard tetanus suppresses LTP induction (LTP suppression). We investigated LTP, DP, and LTP suppression of both field excitatory postsynaptic potentials and population spikes in CA1 neurons of mice lacking the inositol 1,4,5-trisphosphate (IP3) receptor (IP3R)-binding protein released with IP3 (IRBIT). The mean magnitudes of LTP induced by short and standard tetanus were not different in mutant and wild-type mice. In contrast, DP and LTP suppression were attenuated in mutant mice, whereby the mean magnitude of responses after LFS or tetanus were significantly greater than in wild-type mice. These results suggest that, in hippocampal CA1 neurons, IRBIT is involved in DP and LTP suppression, but is not essential for LTP. The attenuation of DP and LTP suppression in mice lacking IRBIT indicates that this protein, released during or after priming stimulations, determines the direction of LTP expression after the delivery of subsequent stimulations.
Dissertations / Theses on the topic "Ltp protein"
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Hurst, Katrina M. "Modulation of Synaptic Plasticity: Endocannabinoids and Novel G-protein Coupled Receptors Expression and Translational Effects in Interneurons." BYU ScholarsArchive, 2017. https://scholarsarchive.byu.edu/etd/6940.
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Learning and memory are important processes that occur in the brain. The brain is comprised of neurons that make connections with each other known as synapses. Synaptic plasticity is widely believed to be the physiologic mechanism by which learning and memory occur. Synapses can either be strengthened through a process known as long-term potentiation (LTP) or weakened through long-term depression (LTD). The area of the brain that is most studied for its role in learning and memory is the hippocampus, which has been shown to be involved in memory consolidation. The detection of endocannabinoids and their receptors has opened a whole new field of study in regards to synaptic plasticity. Cannabinoid receptor 1 (CB1) and transient receptor potential vanilloid 1 (TRPV1) are among the commonly studied endocannabinoid receptors found in the central nervous system. In the brain, these receptors' natural ligands, anandamide and 2-arachidonylglycerol (2-AG), are found in abundance. Yet not all forms of observed plasticity are accounted for by just these two receptors, so studies into other G-protein coupled receptors (GPCRs) continues. One GPCR, GPR55 is found in many regions of the brain, as well as lysophosphatidylinositol (LPI), its specific ligand. Here we have researched the role of GPR55 in modulating synaptic plasticity in the hippocampus. Using quantitative reverse transcription PCR and immunohistochemistry, we have found GPR55 to be expressed in the hippocampus with highest expression in pyramidal cells, the main excitatory neurons in the hippocampus. Using field and whole cell electrophysiology, we have investigated its effects on synaptic plasticity, discovering that activation of GPR55 by LPI significantly enhances LTP. In memory behavioral assays there are no significant differences between GPR55 KO mice and wild type littermates, indicating that it may not be involved in endogenous memory processes. However, our electrophysiology data makes GPR55 a potential target for treating memory disorders such as dementia. We have also investigated GPR18 and GPR119 for their potential roles in synaptic plasticity. First, we confirmed their expression in the hippocampus and then investigated the effects of their agonists on plasticity. Another receptor, TRPV1 has been studied to alter plasticity. However, the study of how protein translation and RNA transcription involvement in TRPV1 plasticity in mammals has not been investigated. While translation and transcription are known to be important in many forms of LTP, it is unknown whether these processes are important for TRPV1-induced LTD. We are investigating their necessity via whole cell patching and using translation and transcription inhibitors Anisomycin and Actinomycin D, both previously used in slice electrophysiology.
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PII, Youry. "Involvement of auxin and LTP proteins in the regulation of root nodule formation in Medicago truncatula - Sinorhizobium meliloti Symbiosis." Doctoral thesis, Università degli Studi di Verona, 2009. http://hdl.handle.net/11562/337398.
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Questo progetto di dottorato ha avuto come obiettivi: i) valutazione del ruolo del’auxina di derivazione batterica nella simbiosi rizobio-leguminosa, che dà origine a noduli di tipo indeterminato, ii) lo studio funzionale di MtN5, una proteina di tipo “Pathogenesis Related”, che viene indotta precocemente durante la nodulazione e che presenta omologie di sequenza con membri della famiglia delle Lipid Transfer Protein vegetali.
L’auxina (acido indol-3-acetico, IAA) è un ormone vegetale implicato in molti aspetti che riguardano la vita e lo sviluppo delle piante; un suo coinvolgimento nello sviluppo del nodulo radicale era stato ipotizzato già all’inizio del secolo scorso. Studi successivi hanno dimostrato un’inibizione del trasporto acropeto di IAA nella radice a seguito dell’infezione con rizobi, con un conseguente accumulo di fitormone a livello del sito di infezione. E’ stato dimostrato che la maggior parte dei batteri della rizosfera che producono effetti di promozione sulla crescita della pianta, rizobi inclusi, possiedono vie biosintetiche per IAA. I rizobi sono in grado di sintetizzare auxina in coltura liquida e, molto probabilmente, mantengono questa capacità anche durante la nodulazione. Ad oggi, però, i dati riguardanti il ruolo dell’auxina batterica nello sviluppo dei noduli sono ancora controversi; sono stati infatti documentati sia effetti stimolatori che inibitori.
Molti degli eventi che stanno alla base dell’associazione simbiotica tra rizobi e leguminose devono ancora essere chiariti. Ad esempio, la natura e la funzione dei segnali ormonali scambiati tra ospite e simbionte non sono ancora stati compresi nel dettaglio, così come le differenze e i parallelismi nella risposta delle leguminose verso il simbionte e verso i patogeni della radice. A tal riguardo, recenti osservazioni hanno dimostrato che la repressione della via di segnalazione intracellulare dell’auxina risulta in una maggiore resistenza innata delle piante verso microrganismi patogeni.
Piante di Medicago truncatula, specie modello per le leguminose che producono noduli di tipo indeterminato, e Medicago sativa (erba medica), specie correlata di interesse agronomico, sono state nodulate sia con rizobi wild-type e che con rizobi in grado di iper-produrre IAA (S. meliloti IAA). I risultati ottenuti hanno dimostrato che piante nodulate con S. meliloti IAA producevano un numero maggiore di noduli (aumento del 50% in M. sativa e
aumento del 100% in M. truncatula) e un apparato radicale più ramificato. Inoltre il contenuto di auxina nei noduli prodotti da rizobi IAA è mediamente 10 volte superiore alla concentrazione dei noduli prodotti da rizobi wild-type. I livelli di espressione dei geni responsabili del trasporto di auxina è stato valutato mediante RT-PCR quantitativa (qRT-PCR) e il carrier di efflusso MtPIN2 è risultato significativamente più espresso (circa 2 volte) nel tessuto radicale di piante nodulate con rizobi IAA rispetto alle radici infettate con il rizobio di controllo. Questi risultati suggeriscono che l’effetto di promozione osservato sulla nodulazione e sull’accrescimento della radice laterale siano dovuti alla produzione di IAA nel nodulo e ad una sua redistribuzione all’interno dell’apparato radicale.
E’ stato ampiamente dimostrato che l’ossido nitrico (NO) agisce come secondo messaggero nell’induzione di radici laterali e avventizie stimolata da auxina. Considerando la comune organogenesi tra radici laterali e avventizie e noduli indeterminati, in questo lavoro abbiamo dimostrato che esiste un collegamento tra auxina e NO nella via di segnalazione che porta all’induzione del nodulo.
Per mezzo di uno screening preliminare, condotto mediante qRT-PCR e volto ad individuare geni differenzialmente espressi in piante nodulate con rizobi IAA e piante nodulate con rizobi wild-type, fu osservato che il gene MtN5 era più espresso negli apparati radicali di piante infettate con rizobi iperproduttori di auxina. Il prodotto genico di MtN5 è stato annotato come una Lipid Transfer Protein (LTP) putativa. Le LTP vegetali sono caratterizzate dalla capacità sia di legare lipidi in vitro che di inibire la crescita di microrganismi. In questo progetto di tesi è stato dimostrato che MtN5 possiede la capacità di legare lisolipidi e che, come molti altri membri di questa famiglia di proteine, possiede attività antimicrobica in vitro, in particolare contro Fusarium semitectum, Xanthomonas campestris e S. meliloti.
Lo studio del profilo di espressione conferma che MtN5 viene precocemente indotta durante la nodulazione e che è specificamente localizzata all’interno del nodulo radicale. Inoltre, l’infezione di piante con F. semitectum provoca l’accumulo di MtN5 nel tessuto radicale.
La funzione di MtN5 nella nodulazione è stata studiata mediante la generazione di radici avventizie transgeniche, sia overesprimenti che silenziante per il gene di interesse. Le radici
silenziate per MtN5 sviluppano circa la metà dei noduli rispetto a radici di controllo, mentre in radici transgeniche over-esprimenti MtN5 il numero di noduli è risultato incrrementato del 300% rispetto al controllo. I risultati ottenuti dimostrano che MtN5 facilita l’interazione simbiotica tra M. truncatula e S. meliloti, agendo probabilmente negli stadi precoci dell’infezione, e suggeriscono che MtN5 potrebbe avere un ruolo nella protezione dei noduli verso patogeni della radice. Ulteriori studi sono comunque necessari per ottenere una immagine più chiara del ruolo di MtN5 sia nella simbiosi che nella risposta verso i patogeni.The present thesis has had two main focuses: i) the evaluation of the role of bacteria-derived auxin in the symbiosis between rhizobia and legumes that bear indeterminate nodules, ii) the functional study of MtN5, a pathogenesis related protein which presents sequence homology with the members of the plant Lipid Transfer Proteins (LTP) family and is precociously induced during nodulation. Auxin (indol-3-acetic acid, IAA) is a phytohormone involved in many aspects of plants growth and development; The role of auxin in the development of the rhizobia-legumes symbiosis was first hypothesised at the beginning of the twentieth century. More recent studies have demonstrated that auxin is accumulated at the site of infection as a consequence of the inhibition of the acropetal auxin transport in roots upon rhizobia inoculation. The production of IAA has also been documented in plant-associated rhizobacteria, including rhizobia, that have promoting effects on plants growth. When grown in liquid media, rhizobia can synthesize auxin and most likely they retain the same capability also during the nodule development. However, up to date, the data concerning the role of bacteria-derived auxin in the establishment of the symbiotic association are still contradictory, since both stimulatory and inhibitory effects have been documented. Thus, there are still open questions in the understanding of the events that result in the establishment of the symbiosis. First of all the nature and the function of the hormonal signal(s) exchanged between the host and the symbiont are not thoroughly unfolded, as well as the parallelisms and the differences in the responses of legumes against root pathogens and root symbiont. In these regards, recent findings have pointed out that plants innate immunity results, at least in part, from the down-regulation of the auxin signalling pathway. Medicago truncatula and Medicago sativa plants were nodulated with both wild-type and auxin hyper-synthesising rhizobia (Sinorhizobium meliloti IAA). The results obtained showed that plants nodulated with S. meliloti IAA produced a higher number of root nodules (50% more nodules in M. sativa and 100% more nodules in M. truncatula) and a more branched root apparatus. The root nodules elicited by S. meliloti IAA had a higher IAA content (at least 10-fold) when compared to control nodules. The expression levels of the auxin carriers were evaluated and the efflux facilitator MtPIN2 resulted more abundant (about 2-fold) in the root tissue of IAA plants when compared to wild-type plants These data suggested that such promoting effects on nodulation and lateral root growth might be due to the increased auxin content detected in IAA nodule produced by auxin hyper-synthesising rhizobia, as well as to a redistribution of the phytohormone in the root tissue. It has been largely demonstrated that nitric oxide (NO) acts as second messenger in the auxin-induced pathway that leads to formation of lateral and adventitious roots. Since root nodules have the same organogenetic origin of lateral and adventitious roots, the possible connection between NO and root nodule induction was investigated and we demonstrated that NO participate in the signalling pathway for root nodule induction. During a preliminary screening carried out by means of qRT-PCR, it has been found that N5 gene of M. truncatula was more abundantly expressed in roots nodulated with S. meliloti IAA with respect to roots infected by wild-type rhizobia. The gene product of MtN5 was annotated as putative Lipid Transfer Protein (LTP). LTPs are characterized by their ability to bind lipids in vitro and the majority of them exhibits antimicrobial activity. In this thesis, it has been demonstrated that the recombinant MtN5 protein is able to bind lysolipids and possesses inhibitory activity against Fusarium semitectum, Xanthomonas campestris and S. meliloti. The studies of the expression pattern of both MtN5 transcript and MtN5 protein confirmed that it is precociously induced during nodulation and revealed that it is specifically localized in the root nodule. In addition, when M. truncatula plants are infected with the root pathogenic fungus F. semitectum, MtN5 protein is accumulated in the root apparatus. The function of MtN5 in nodulation has been studied through the generation of transgenic adventitious roots, both over-expressed and silenced for the gene of interest. MtN5-silenced roots developed approximately 50% fewer nodules as compared to control roots, whereas in hairy roots over-expressing MtN5 the nodule number was increased by about 300% with respect to control roots. Collectively the data indicate that MtN5 facilitates the symbiotic interaction between M. truncatula and S. meliloti, probably acting in the early stages of rhizobia infection, and suggest that it might have a role in the protection of nodules against root pathogen. However, further studies are needed to have a clear picture of the role played by MtN5 in both symbiosis and defence response against pathogens.
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Andrews, Shantaya. "Localization of SIP470, a Plant Lipid Transfer Protein in Nicotiana tabacum." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etd/3520.
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SABP2-interacting protein 470 (SIP470), a non-specific lipid transfer protein (nsLTP), was discovered in a yeast two-hybrid screening using SABP2 as bait and tobacco leaf proteins as prey. SABP2 is an important enzyme in systemic acquired resistance that converts salicylic acid to methyl salicylate. Localization studies are an important aspect to understanding the biological function of proteins. nsLTPs are generally considered apoplastic proteins and has been localized intracellularly and extracellularly. Transient expression shows highest expression of SIP470-eGFP at 2 days post infiltration into Nicotiana benthamiana. Confocal microscopy showed localization near the periphery of the cell. Subcellular localization using differential centrifugation showed that SIP470 is localized in the mitochondria. Mitochondria membranes are rich in lipids and have shown lipid exchange with the endoplasmic reticulum in mammalian systems. Co-localization of SIP470-eGFP+mCherry did not express complete co-localization in the targeted organelles. Co-localization pattern suggests possible localization in the endoplasmic reticulum.
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Silva, Renan Gonçalves da. "Análise in silico do gene lipid transfer protein (LTP) de cana-de-açúcar e funcional em transformantes de (Nicotiana tabacum) /." Jaboticabal, 2017. http://hdl.handle.net/11449/151990.
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Orientador: Sonia Marli ZingarettiBanca: janete Apparecida Desiderio
Banca: Juliana da Silva Coppede
Resumo: A grande expansão da cultura de cana-de-açúcar pelo território brasileiro leva à necessidade do desenvolvimento de cultivares melhoradas e adaptadas às diferentes condições de clima a que são submetidas. Os estresses bióticos e abióticos são fatores que afetam a produtividade de uma cultura e entre esses, o estresse hídrico assume grande importância em função do regime de chuvas e do aumento de temperatura iminente nos próximos anos. Analisar a expressão de genes em plantas submetidas a estresses pode contribuir de forma expressiva para elucidar as rotas de defesa das plantas, contribuindo sobremaneira para o melhoramento da cultura e o desenvolvimento de novas variedades. O projeto teve como objetivo obter transformantes de Nicotiana tabacum in vitro com o gene LTP (Lipid Transfer Protein) e avaliar sua funcionalidade em relação ao estresse por deficiência hídrica, em casa de vegetação por hidroponia. Feita a seleção da EST com base em resultados anteriores obtidos pelo grupo de pesquisa, posterior análise em bancos de dados, realizou-se a aquisição do clone no Centro de Estocagem de Genes (BCCCenter) e o re-sequenciamento para comprovar sua identidade. Estudos in silico foram realizados através da utilização de softwares de bioinformática e a análise da função do gene foi realizada a partir da transformação genética de Nicotiana tabacum via Agrobacterium tumefaciens. Seis transformantes de N. tabacum com o inserto de interesse LTP foram obtidos e testados quanto à tolerânci... (Resumo completo, clicar acesso eletrônico abaixo)
The great expansion of sugarcane cultivation across Brazilian territory leads to the need to develop better cultivars and adapted to the different climatic conditions that are submitted. The biotic and abiotic stresses are factors that affect the productivity of a crop and among them, the water stress will assume great importance due to the rainfall regime and the increase of the imminent temperature in the next years. Analyzing the expression of genes in stress - stressed plants can contribute in an expressive way to elucidate as plant defense routes, contribute to the improvement of the culture and the development of new varieties. The objective of this project was to obtain transformers of Nicotiana tabacum in vitro with the LTP (Lipid Transfer Protein) gene and to evaluate its functionality in relation to stress due to water deficiency in a greenhouse by hydroponics. We made EST selection based on previous results obtained by a research group, later analysis in databases, performing a clone acquisition in the Gene Storage Center (BCCCenter) and resequencing to prove its identity. Silicon studies were carried out through the application of bioinformatics software and an analysis of the genetic function was performed from the genetic transformation of Nicotiana tabacum via Agrobacterium tumefaciens. Six N. tabacum transformants with the LTP insert of interest were obtained and tested for tolerance to water deficit by induction of different concentrations of mannitol. Transformer tobacco plants showed better phenotypic performance compared to untransformed plant and good readaptation after stress. The T1 generation these plants will be used in studies for the biological and functional verification of the action of the inserted gene, through the Real-Time qPCR technique.
Mestre
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Mohammad, Sameh. "Long-term depression in the rat hippocampus as a memory model : Interrogating the role of protein synthesis in NMDA- and mGluR-dependent synaptic plasticity." Thesis, Högskolan i Skövde, Institutionen för vård och natur, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-4715.
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Long-term potentiation (LTP) and depression (LTD) are important forms of activity-dependent synaptic plasticity believed to play a role in memory at the cellular level. It has previously been described that synthesis of new proteins is needed to maintain LTP longer than a few hours. Other reports argue that sufficient proteins for stable LTP are already available. The present study aims to examine the role of protein synthesis in LTD, the presumed mirror mechanism of LTP. Experiments were carried out in hippocampal slices from young (12-45 days) and old (12-18 weeks) Sprague-Dawley rats. Extracellular techniques were used to study synaptic responses in the Schaffer-collateral-commissural pathway. Plasticity was induced electrically by low frequency stimulation (2-3 trains at 1 Hz for 15 min) or chemically by brief exposure to certain glutamate receptor agonists (NMDA at 20 µM for 3 min or DHPG at 100 µM for 10 min). Whole slice protein synthesis was quantified by assessing 3H-leucine incorporation. Stable LTD (> 8 h) was be obtained by either electrical or chemical activation. Protein synthesis inhibitors anisomycin (40 uM) and cycloheximide (100 uM) both failed to influence the magnitude of LTD. Moreover, no age difference was found, in terms of stable LTD in both young and old rats under inhibition of protein synthesis. The potency of the inhibitors was found to be high, depressing synthesis down to a few percent. It is concluded that sufficient proteins for generating stable LTD are normally present in the brain, implying a large safety-margin for cellular memory.
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Pascal, i. Capdevila Mariona. "Allergenic protein and epitope recognition in food allergy: a new perspective for the molecular and clinical characterization of shellfish and lipid transfer protein allergy / Reconeixement de proteïnes i epítops al•lergènics en al•lèrgia alimentaria: una nova perspectiva per a la caracterització clínica i molecular de l’al•lèrgia al marisc i a les proteïnes de transferència de lípids." Doctoral thesis, Universitat de Barcelona, 2012. http://hdl.handle.net/10803/84070.
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Currently food allergy diagnostic tests are not able to predict clinical reactivity in sensitized patients (those with specific IgE against a particular allergen). Traditionally, allergy diagnostic tests used complete extracts of allergenic sources containing multiple molecules, some allergic and others not. This greatly limits the accuracy in the diagnosis and identification of possible allergic reactions to different foods by the existence of cross reactivity. Through the last decades, thanks to advances in the characterization of allergens at molecular level, the concept of Component-Based Diagnosis has been developed. This is based on the reasoning that the presence of specific IgE for the protein actually responsible for the allergic response should be detected and not the one against a mixture of molecules. Furthermore, the study of IgE and IgG4 recognition at epitope level using microarrays of synthetic peptides has been described as a useful tool for diagnosis, prognosis and development of a therapy for food allergy.
The hypothesis of this thesis is that these new methodologies can improve the diagnosis of shellfish and lipid transfer protein (LTP) allergy. The aim of this thesis is to characterize clinically and at a molecular level these two types of food allergies, using these new methodologies.
Regarding shellfish allergy, we found that tropomyosin, sarcoplasmic protein binding of calcium and myosin light chain are the allergens associated with clinical reactivity, i.e, are more common in shrimp allergic patients than in tolerant individuals sensitized shrimp. On the other hand, arginine kinase and hemocyanin allergens would be more involved in the phenomena of cross-reactivity with other arthropods (mites and/or cockroach). Additionally the synthetic peptide microarray has identified differential recognition of IgE and IgG4 epitopes among allergic and tolerant individuals. Regarding allergy to LTP, allergenic proteins ubiquitously distributed in the plant kingdom, we observed that patients suffer from reactions with a wide range of plant foods to which are sensitized, being peach the most common one. Furthermore, these patients have a variety of clinical symptoms from very mild to very severe and life-threatening as in the case of anaphylaxis, which can be attributed to allergens from different families. The component-based diagnosis in a microarray format that includes a diverse panel of allergenic proteins from different families is useful for diagnosing these patients, since the only proteins identified as responsible for the clinical symptoms are the LTPs, although the symptoms are diverse and sometimes more closely resemble to those caused by other allergens such as profilins or homologues of Bet v 1. Moreover, it offers an overview of positive and negative sensitivities in these patients in a single trial, with its multiplex properties. Cases of anaphylaxis with a cofactor involvement, such as NSAIDs, were frequently observed in these patients. The simultaneous presence of these drugs with the food allergen triggers allergic reactions in the individual that would not occur without the presence of the drug or would have been of less severity. We have developed a preliminary in vitro model based on the basophil activation test that allowed us to observe the in vitro effect observed in vivo. We observed an increase of degranulation/activation of basophils when stimulation is done with food in the presence the drug, compared to when stimulated only with food.
In this thesis we can conclude that both the component-based diagnosis and epitope mapping are useful tools for the characterization of food allergy to shellfish proteins and LTP, and that they should be considered to improve the efficiency of diagnosis of these two types of food allergies.Actualment els mètodes diagnòstics de l'al•lèrgia alimentària no són capaços de predir la reactivitat clínica dels pacients sensibilitzats (els que tenen IgE específica davant un determinat al•lergen). Tradicionalment les proves diagnòstiques de l'al•lèrgia han utilitzat extractes complets de fonts al•lergèniques que contenen múltiples molècules, algunes al•lergèniques i altres no. Això limita enormement la precisió en el diagnòstic i la possibilitat d'identificar reaccions al•lèrgiques a diferents aliments per l'existència de reactivitats creuades. Gràcies a la caracterització dels al•lèrgens a nivell molecular, s'ha desenvolupat el concepte del Diagnòstic Basat en Components que es basa en el raonament de detectar la presència d'IgE específica per a la proteïna realment responsable de la resposta al•lèrgica i no per una mescla de molècules. Addicionalment, l'estudi del reconeixement IgE i IgG4 a nivell d'epítops amb microarrays de pèptids sintètics pot ser una eina útil per al diagnòstic, pronòstic i desenvolupament d'una teràpia per l'al•lèrgia alimentària. La hipòtesi d'aquesta tesi és que aquestes noves metodologies poden millorar el diagnòstic de l'al•lèrgia al marisc i a les proteïnes de transferència de lípids (LTP), presents en múltiples aliments vegetals. L'objectiu és doncs caracteritzar clínicament i a nivell molecular aquests dos tipus d'al lèrgies alimentàries, utilitzant aquestes noves metodologies. Respecte a l'al•lèrgia al marisc, els al lèrgens tropomiosina, proteïna sarcoplàsmica d'unió de calci i la cadena lleugera de miosina s'associen amb la reactivitat clínica a la gamba. D'altra banda, els al•lèrgens arginina quinasa i hemocianina estarien més implicats en fenòmens de reactivitat creuada amb altres artròpodes. Addicionalment, amb el microarray de pèptids sintètics s'ha pogut identificar un reconeixement diferencial d'epítops IgE i IgG4 entre pacients al•lèrgics i tolerants. Respecte a l'al•lèrgia a les LTP, els pacients pateixen reaccions amb un ampli ventall d'aliments vegetals, sent el préssec el més freqüent, amb una gran diversitat de símptomes clínics, que poden atribuir-se a al•lèrgens de diferents famílies. El diagnòstic basat en components en el format d'un microarray que inclou proteïnes al•lergèniques de diferents famílies és útil per al diagnòstic d'aquests pacients, ja que permet identificar que les úniques proteïnes responsables els símptomes clínics són les LTP, encara que els símptomes siguin molt variats i en algunes ocasions s'assemblin més als provocats per altres al•lèrgens com les profilines o els homòlegs de Bet v 1. En aquests pacients són freqüents els casos d'anafilàxia en què està involucrat un cofactor, com els antiinflamatoris no esteroïdals. La presència del fàrmac amb l'al•lergen alimentari desencadena reaccions al•lèrgiques que sense el fàrmac no es donarien o serien de menor severitat. Hem desenvolupat un model preliminar in vitro basat en el test d'activació de basòfils que ens ha permès observar in vitro l'efecte observat in vivo. En conclusió, el diagnòstic basat en components i el mapatge d'epítops són eines útils per a la caracterització de l'al•lèrgia alimentària al marisc i a les proteïnes LTP, i s'han de considerar per millorar l'eficiència del diagnòstic d'aquests dos tipus d'al•lèrgies alimentàries.
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Audam, Timothy Ndagi. "Characterization of SIP470, a Family 1 Lipid Transfer Protein and its Role in Plant Stress Signaling." Digital Commons @ East Tennessee State University, 2016. https://dc.etsu.edu/etd/3103.
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SIP470, a putative tobacco lipid transfer protein, was identified in a yeast two-hybrid screen to interact with SABP2. SABP2 is a critical role in SA-mediated signaling in tobacco and other plants. In vitro studies using purified recombinant SIP470 confirmed that it is a lipid binding protein. In an attempt to determine its role in mediating stress responses, Arabidopsis T-DNA insertion knockout lines lacking SIP470 homolog were used for the analysis. These mutant plants were defective in basal resistance against microbial pathogens. Expression of defense gene PR-1 was also delayed in these mutant plants. Interestingly, these mutant plants were not defective in inducing systemic acquired resistance. Besides biotic stress, these mutant plants also showed increased susceptibility to abiotic stresses. To directly study the role of SIP470 in tobacco plants, transgenic tobacco lines, with reduced levels of SIP470 expression, were generated using RNAi and transgenic lines overexpressing SIP470 were also generated.
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CENTINI, BARBARA. "Correlazione tra deterioramento cognitivo, plasticità sinaptica corticale e livelli liquorali di amiloide-β1-42 nella Sclerosi multipla." Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2009. http://hdl.handle.net/2108/208702.
Full textAbstract:
La presenza di disturbi cognitivi nei pazienti con Sclerosi Multipla (MS) era già stata descritta nel 1877 da Charcot; comunque solo negli ultimi 30 anni si è assistito ad una attenzione degli studiosi in merito alla comprensione delle compromissioni cognitive quantitative e qualitative presenti in questi pazienti. Circa il 45-65% dei pazienti con MS mostra disfunzioni cognitive di una certa entità, tali disturbi vanno da disturbi selettivi di specifiche funzioni a una compromissione grave e diffusa. Le disfunzioni cognitive frequentemente osservate nei pazienti con MS, sono associate con alterazioni della materia grigia, atrofia cerebrale e alterazioni della connettività e plasticità sinaptica. Recenti studi mostrano come anchvi e l'infiammazione acuta può esacerbare i deficit cognitivi indipendentemente dal sistema funzionale primariamente coinvolto.In questo studio noi misuriamo i livelli di amiloide beta 1-42 e proteina tau in pazienti con MS e CIS (Clinical Isolated Syndrome) , da sempre le due proteine sono state associate con il deterioramento cognitivo presente nei pazienti con malattia di Alzheimer (AD). Nella AD, l'amiloide beta 1-42 si accumula nel tessuto nervoso in placche extracellulari insolubili; questo fenomeno rende possibile la spiegazione del fatto che la forma solubile della beta 1-42 amiloide sia ridotta nel liquido cefalo rachidiano di questi pazienti. Nel nostro campione di pazienti affetti da MS, i livelli di amiloide beta 1-42 erano significativamente più bassi in pazienti con deficit cognitivi e erano inversamente correlati con il numero di lesioni GD+ alle immagini della risonanza magnetica (MRI). Sono state inoltre evidenziate correlazioni positive tra i livelli di amiloide beta 1-42 e i deficit di attenzione e concentrazione. Inoltre, l'anormale neuroplasticità della corteccia cerebrale è stata esplorata con TBS (theta brust magnetic stimulation). Da questa analisi è emerso che in pazienti con deficit cognitivi c'è una correlazione positiva tra i livelli di amiloide beta 1-42 presenti nel CSF e l'ampiezza dei long-term-potentiation (LPT) indotti dalla TBS. Nessuna correlazione è stata invece individuata tra la concentrazione della proteina tau, MRI, parametri cognitivi e gli effetti della TBS in questi pazienti. Insieme questi risultati indicano che nella MS l'infiammazione del SNC è capace di alterare il metabolismo della beta amiloide, riducendo la sua concentrazione nel liquido cerebro spinale e portando ad un danneggiamento della plasticità sinaptica e delle funzioni cognitive.Cognitive dysfunction is of frequent observation in Multiple Sclerosis (MS). It is associated with grey matter pathology, brain atrophy and altered connectivity, and recent evidence showed that acute inflammation can exacerbate mental deficits independently of the primary functional system involved. In the present study, we measured cerebrospinal fluid (CSF) levels of amyloid-1-42 and tau protein in MS and in Clinical Isolated Syndrome (CIS) patients, since both proteins have been associated with cognitive decline in Alzheimer's disease (AD). In AD, amyloid-1-42 accumulates in the brain as insoluble extracellular plaques, possible explaining why soluble amyloid-ß1–42 is reduced in the CSF of these patients. In our sample of MS patients, amyloid-1-42 levels were significantly lower in patients cognitively impaired and were inversely correlated with the number of Gadolinium-enhancing (Gd+) lesions at the magnetic resonance imaging (MRI). Positive correlations between amyloid-1-42 levels and measures of attention and concentration were also found. Furthermore, abnormal neuroplasticity of the cerebral cortex, explored with theta burst magnetic stimulation (TBS), was observed in cognitively impaired patients, and a positive correlation was found between amyloid-1-42 CSF contents and the magnitude of long-term potentiation (LTP)-like effects induced by TBS. No correlation was conversely found between tau protein concentrations and MRI findings, cognitive parameters, and TBS effects in these patients. Together, our results indicate that in MS central inflammation is able to alter amyloid- metabolism, by reducing its concentration in the CSF, and leading to impairment of synaptic plasticity and cognitive function.
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Graupner, Michael. "Induction and Maintenance of Synaptic Plasticity." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2008. http://nbn-resolving.de/urn:nbn:de:bsz:14-ds-1221145787153-31869.
Full textAbstract:
Synaptic long-term modifications following neuronal activation are believed to be at the origin of learning and long-term memory. Recent experiments suggest that these long-term synaptic changes are all-or-none switch-like events between discrete states of a single synapse. The biochemical network involving calcium/calmodulin-dependent protein kinase II (CaMKII) and its regulating protein signaling cascade has been hypothesized to durably maintain the synaptic state in form of a bistable switch. Furthermore, it has been shown experimentally that CaMKII and associated proteins such as protein kinase A and calcineurin are necessary for the induction of long-lasting increases (long-term potentiation, LTP) and/or long-lasting decreases (long-term depression, LTD) of synaptic efficacy. However, the biochemical mechanisms by which experimental LTP/LTD protocols lead to corresponding transitions between the two states in realistic models of such networks are still unknown. We present a detailed biochemical model of the calcium/calmodulin-dependent autophosphorylation of CaMKII and the protein signaling cascade governing the dephosphorylation of CaMKII. As previously shown, two stable states of the CaMKII phosphorylation level exist at resting intracellular calcium concentrations. Repetitive high calcium levels switch the system from a weakly- to a highly phosphorylated state (LTP). We show that the reverse transition (LTD) can be mediated by elevated phosphatase activity at intermediate calcium levels. It is shown that the CaMKII kinase-phosphatase system can qualitatively reproduce plasticity results in response to spike-timing dependent plasticity (STDP) and presynaptic stimulation protocols. A reduced model based on the CaMKII system is used to elucidate which parameters control the synaptic plasticity outcomes in response to STDP protocols, and in particular how the plasticity results depend on the differential activation of phosphatase and kinase pathways and the level of noise in the calcium transients. Our results show that the protein network including CaMKII can account for (i) induction - through LTP/LTD-like transitions - and (ii) storage - due to its bistability - of synaptic changes. The model allows to link biochemical properties of the synapse with phenomenological 'learning rules' used by theoreticians in neural network studies.
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Graupner, Michael. "Induction and Maintenance of Synaptic Plasticity." Doctoral thesis, Technische Universität Dresden, 2007. https://tud.qucosa.de/id/qucosa%3A23857.
Full textAbstract:
Synaptic long-term modifications following neuronal activation are believed to be at the origin of learning and long-term memory. Recent experiments suggest that these long-term synaptic changes are all-or-none switch-like events between discrete states of a single synapse. The biochemical network involving calcium/calmodulin-dependent protein kinase II (CaMKII) and its regulating protein signaling cascade has been hypothesized to durably maintain the synaptic state in form of a bistable switch. Furthermore, it has been shown experimentally that CaMKII and associated proteins such as protein kinase A and calcineurin are necessary for the induction of long-lasting increases (long-term potentiation, LTP) and/or long-lasting decreases (long-term depression, LTD) of synaptic efficacy. However, the biochemical mechanisms by which experimental LTP/LTD protocols lead to corresponding transitions between the two states in realistic models of such networks are still unknown. We present a detailed biochemical model of the calcium/calmodulin-dependent autophosphorylation of CaMKII and the protein signaling cascade governing the dephosphorylation of CaMKII. As previously shown, two stable states of the CaMKII phosphorylation level exist at resting intracellular calcium concentrations. Repetitive high calcium levels switch the system from a weakly- to a highly phosphorylated state (LTP). We show that the reverse transition (LTD) can be mediated by elevated phosphatase activity at intermediate calcium levels. It is shown that the CaMKII kinase-phosphatase system can qualitatively reproduce plasticity results in response to spike-timing dependent plasticity (STDP) and presynaptic stimulation protocols. A reduced model based on the CaMKII system is used to elucidate which parameters control the synaptic plasticity outcomes in response to STDP protocols, and in particular how the plasticity results depend on the differential activation of phosphatase and kinase pathways and the level of noise in the calcium transients. Our results show that the protein network including CaMKII can account for (i) induction - through LTP/LTD-like transitions - and (ii) storage - due to its bistability - of synaptic changes. The model allows to link biochemical properties of the synapse with phenomenological 'learning rules' used by theoreticians in neural network studies.
Books on the topic "Ltp protein"
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Gideon, Alexander, and European Organization for Nuclear Research., eds. Polarization at LEP. Geneva: CERN, European Organization for Nuclear Research, 1988.
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Nicolas, Rey, ed. LKP: Guadeloupe : le mouvement des 44 jours. Paris: Syllepse, 2010.
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G, Altarelli, Kleiss Ronald, and Verzegnassi Claudio, eds. Z physics at LEP 1. Geneva: CERN, European Organization for Nuclear Research, 1989.
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author, Kaulanjan-Diamant Axelle, ed. Abécédaire LKP: Clés analytiques et critiques du mouvement : enquête journalistique, analyse sociologique. Matoury, Guyane: Ibis Rouge Éditions, 2012.
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Praag, Philip, ed. Political Science and Changing Politics. NL Amsterdam: Amsterdam University Press, 2017. http://dx.doi.org/10.5117/9789462987487.
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Politics is about conflict, struggle, decision-making, power and influence. But not every conflict and not every situation in which power is exercised is widely regarded as politics. A football coach who decides to leave a player on the bench because he has given him a bit of lip, is exerting power, and there is conflict here, too. However, few people would consider this a political issue. The same applies to a mother who quarrels with her adolescent daughter about going to a house party, a schoolteacher who gives a student detention, and so on. But if we were to limit our understanding of politics to official decisions that are taken by governments, in parliaments or on municipal councils, we would fail to recognise the political meaning of trade unions, lobbyists, protest groups, corporations and other more-or-less organised groups that influence collective decision-making.
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Moseley, V. J. "Jon", Andreas Lampropoulos, Eftychia Apostolidi, and Christos Giarlelis. Characteristic Seismic Failures of Buildings. Edited by Stephanos E. Dritsos. Zurich, Switzerland: International Association for Bridge and Structural Engineering (IABSE), 2019. http://dx.doi.org/10.2749/sed016.
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<p>Earthquakes can cause considerable fatalities, injuries and financial loss. The forces of nature cannot be blamed, as the problem lies with the structures in seismic regions that may not have been designed or constructed to a sufficient degree to resist earthquake actions or they may have design flaws. This Structural Engineering Document (SED) concerns reinforced concrete and masonry buildings together with geotechnical aspects and presents in a concise and practical way the state of the art of current understanding of building failures due to earthquakes. It classifies the different types of seismic failure, explains the reasons for each failure, describes good practices to avoid such failures and also describes seismic retrofitting/upgrading procedures for pre-earthquake strengthening and post-earthquake repair and/or strengthening techniques for deficient buildings. Carefully selected photographs and diagrams illustrate the different failure types. This document could be considered as quite unique, as this is the first time such material concerning characteristic seismic failures of buildings has been presented together in one single document. It is intended to be a valuable educational reference textbook aimed at all levels of experience of engineers. It provides background information, ideas, guidance and reassurance to engineers in earthquake regions faced with the task of building a safer future for the public and to protect lives. <p> <iframe width="560" height="315" src="https://www.youtube-nocookie.com/embed/Oddi3VTtxCM" frameborder="0" allow="accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe>
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Fernandez, Elizabeth. Debiry Innovations Ltd. Manufacturers of Protein Fabric. Lulu Press, Inc., 2009.
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Wang, David Derwoei. The role of Epstein-Barr virus latent infection membrane protein (LMP) in cell transformation. 1988.
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How to Plan for Long-Term Care: Protect Your Family with a Smart LTC Plan. Plan Ahead, Inc., 2014.
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Simple LTC Solution: How to Protect Your Life's Savings with a Long-Term Care Partnership Program. Independently Published, 2018.
Find full textBook chapters on the topic "Ltp protein"
1
Malinow, Roberto, and Richard W. Tsien. "Identifying and Localizing Protein Kinases Necessary for LTP." In Excitatory Amino Acids and Neuronal Plasticity, 301–5. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4684-5769-8_33.
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Lullien-Pellerin, V., T. Ihorai, C. Devaux, D. Marion, M. Ptak, P. Joudrier, and M. F. Gautier. "Site-Directed Mutagenesis of Wheat 9 kDa Lipid Transfer Protein (LTP)." In Plant Proteins from European Crops, 88–93. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-662-03720-1_15.
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Michon, T., G. Compoint, J. Douliez, P. Sodano, M. Ptak, and D. Marion. "Lipid-Transfer Protein (LTP) from Wheat Kernel Possesses a Weak, Specific Esterase-like Activity Towards Short Chain Fatty Acid Esters." In Plant Proteins from European Crops, 36–40. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-662-03720-1_6.
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Bollati, Michela, and Louise J. Gourlay. "Protein Crystallization of Two Recombinant Lpt Proteins." In Lipopolysaccharide Transport, 249–63. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2581-1_15.
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Okamoto, Hiroshi, and Kazuhisa Ichikawa. "Amplification of Switching Characteristics of Biochemical-Reaction Networks Involving Ca2+/Calmodulin-Dependent Protein Kinase II: Implication for LTP Induced by a Single Burst during the Theta Oscillation." In Information Processing in Cells and Tissues, 125–36. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-5345-8_14.
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Thresher, J. J. "The LEP Project at CERN." In New Aspects of High-Energy Proton-Proton Collisions, 75–89. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4615-9540-3_3.
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Peterson, Stacey N., and Norbert O. Reich. "LRP: A Nucleoid-Associated Protein with Gene Regulatory Functions." In Bacterial Chromatin, 353–64. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3473-1_15.
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Fiske, Lucy. "Hunger Strike, Lip Sewing and Self-harm." In Human Rights, Refugee Protest and Immigration Detention, 113–46. London: Palgrave Macmillan UK, 2016. http://dx.doi.org/10.1057/978-1-137-58096-2_5.
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Alonso, Nivaldo, and Julia Amundson. "Bone Substitute: Alveolar Bone Grafting (ABG) with rhBMP-2 (Recombinant Bone Morphogenic Protein-2)." In Cleft Lip and Palate Treatment, 263–68. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-63290-2_17.
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Hatzubai, A., M. Anafi, G. Klein, and D. Sulitzeanu. "Expression of the EBV-Encoded Membrane Protein (LMP) in Virally Transformed Cells." In Epstein-Barr Virus and Human Disease, 255–56. Totowa, NJ: Humana Press, 1987. http://dx.doi.org/10.1007/978-1-4612-4590-2_56.
Full textConference papers on the topic "Ltp protein"
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Gonçalves da Silva, Renan, Janeth Silva Pinheiro Marciano, Larissa Cadeo Hulm, and Sonia Marli Zingaretti. "Análise in silico do Gene LTP (Lipid Transfer Protein) sob condições de Estresse Abiótico." In Simpósio de Bioquímica e Biotecnologia. Londrina - PR, Brazil: Galoa, 2017. http://dx.doi.org/10.17648/simbbtec-2017-80906.
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de Vries, C. J. M., N. K. Veerman, and H. Pannekoek. "ARTIFICIAL EXON SHUFFLING: CONSTRUCTION OF HYBRID cDNAS CONTAINING DOMAINS OF TISSUE-TYPE PLASMINOGEN ACTIVATOR (T-PA) AND UROKINASE (u-PA)." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643940.
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The intriguing finding that functions of t-PA coincide with structural domains and that these domains occur in related proteins, has been the basis to construct hybrid proteins by artificial exon shuffling to prove the conservation of functions in the shuffled domains. The heavy chain (Hch) of t-PA mediates both binding to fibrin and stimulation of plasminogen activator activity via its Finger- and Kringle-2 domain, whereas the light chain (Lch) contains the serine protease moiety of the protein. The Hch of u-PA is very homologous to the Lch of t-PA, but exhibits a higher plasminogen activator activity. This activity of u-PA is not stimulated by fibrin. We employed the ‘M13 in vitro outlooping’ technique to fuse the Hch of t-PA cDNA and the Hch of u-PA cDNA, to create two different hybrid cDNAs. On one hybrid cDNA, the t-PA and the u-PA sequences are coupled precisely at the exon-intron boundaries of the corresponding genes, while the other hybrid cDNA lacks a u-PA segment at the junction, encoding 13 amino acids of u-PA. The hybrid cDNAs were transiently expressed in mouse Ltk- cells and the recombinant proteins were characterized. The plasminogen activator activity of these proteins was determined in an indirect amidolytic assay, using plasminogen and the chromogenic substrate S2251. As anticipated, the activity of both t-PA/u-PA hybrid proteins is stimulated by fibrin, however, not to the same extent as t-PA. Remarkably, we found a decreased inhibition of the hybrid proteins by the endothelial plasminogen activator inhibitor (PAI-1) as compared to t-PA and u-PA, although stable complexes between the hybrid proteins and the inhibitor are formed. We conclude that functions of structural domains are maintained during exon shuffling
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Asen, Nancy, and Rotimi Aluko. "Functional Properties of Enzymatic Pea Protein Hydrolysates That Inhibit in vitro Activities of Acetylcholinesterase and Butyrylcholinesterase." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/ktht4252.
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Overview: Alzheimer’s disease (AD) is a neurodegenerative disorder prevalent among the aged population with morbidity and mortality rate of ~ 12%. Research has linked the cause of this disorder to the loss of acetylcholine through excessive activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Therefore, a promising therapeutic approach for AD treatment is the inhibition of AChE/BChE activities. Common features of an AD brain include low levels of acetylcholine, the presence of amyloid-β peptides deposits and severe oxidative stress triggered by lipid peroxidation and formation of free radicals. Natural peptides that possess antioxidant and bioactivities could have prospects for use in AD management. Objectives: Optimize enzymatic hydrolysis of yellow field pea proteins into protein hydrolysates that possess antioxidant, anti-AChE and anti-BChE activities. Methods: Pea protein (70%) was hydrolyzed using six as alcalase (AH), chymotrypsin (CHH), flavourzyme (FZH), pancreatin (PCH), pepsin (PEH) and trypsin (TPH). The supernatants were sequentially passed through ultrafiltration (UF) membranes with molecular weight cut-off of 1, 3, 5 and 10 kDa to collect the permeates as < 1, 1-3, 3-5, and 5-10 kDa, respectively. The hydrolysates and UF fractions were screened for inhibition of linoleic acid peroxidation (LAP) in addition to radical scavenging (hydroxyl and superoxide) and anti-AChE/BChE properties.Results: Hydrolysates showed varying degrees of radicals scavenging and LAP, as well as anti-AChE and anti-BChE activities but the potency improved by >10% for most UF fractions. AH, FZH, PEH and the UF fractions (1-3 kDa) exhibited better and statistically significant (p<0.05) radical scavenging and AChE/BChE activities than other hydrolysates by 20-30% and 20-40% respectively at same concentrations (10-50 µg).Significance of study: The results suggest that pea protein-derived peptides could be potential candidates for use in the inhibition of AChE and BChE activities, which could provide therapeutic tools suitable for the prevention and treatment of AD.
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Дмитриев, Дмитрий, Dmitry Dmitriev, Василий Финогеев, and Vasily Finogeev. "CONTENT OF MAJOR NUTRIENTS AND SOME MACRO AND MICROELEMENTS IN HAYLAGE AND SILAGE OF THE AGRICULTURAL ENTERPRISE "KRASNYY MAYAK" LTD FOR 2016." In Multifunctional adaptive feed production. ru: Federal Williams Research Center of Forage Production and Agroecology, 2019. http://dx.doi.org/10.33814/mak-2019-21-69-98-103.
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In the proposed material the features of the accumulation of basic nutrients is considered, such as crude protein, crude fiber and crude fat, as well as some macro- and microelements in haylage and silage, of the agricultural enterprise CJSC "Krasny Mayak".
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Jin, Hongwei, and Xun Chen. "Gromov-Wasserstein Discrepancy with Local Differential Privacy for Distributed Structural Graphs." In Thirty-First International Joint Conference on Artificial Intelligence {IJCAI-22}. California: International Joint Conferences on Artificial Intelligence Organization, 2022. http://dx.doi.org/10.24963/ijcai.2022/294.
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Learning the similarity between structured data, especially the graphs, is one of the essential problems. Besides the approach like graph kernels, Gromov-Wasserstein (GW) distance recently draws a big attention due to its flexibility to capture both topological and feature characteristics, as well as handling the permutation invariance. However, structured data are widely distributed for different data mining and machine learning applications. With privacy concerns, accessing the decentralized data is limited to either individual clients or different silos. To tackle these issues, we propose a privacy-preserving framework to analyze the GW discrepancy of node embedding learned locally from graph neural networks in a federated flavor, and then explicitly place local differential privacy (LDP) based on Multi-bit Encoder to protect sensitive information. Our experiments show that, with strong privacy protection guaranteed by ε-LDP algorithm, the proposed framework not only preserves privacy in graph learning, but also presents a noised structural metric under GW distance, resulting in comparable and even better performance in classification and clustering tasks. Moreover, we reason the rationale behind the LDP-based GW distance analytically and empirically.
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Biao Liu, Hao-yu Liu, Yan Liu, Xian-yu Zhang, Zhuang Wei, and Zhao-xia Wang. "Localization of S-lap/GFP fusion protein and its effect on cell cycle in 293T cells." In 2011 International Symposium on Information Technology in Medicine and Education (ITME 2011). IEEE, 2011. http://dx.doi.org/10.1109/itime.2011.6132097.
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Jukoski, Tayana Schultz, Talita Helen B. Gomig, Tamyres MIngorance Carvalho, Cicero Andrade Urban, and Enilze Maria Souza Fonseca Ribeiro. "IN SILICO AND PROTEOMICS APPROACHES SUGGEST UPREGULATION OF miR-146a-5p IN TNBC AND MODULATION OF CRITICAL PROTEINS." In Scientifc papers of XXIII Brazilian Breast Congress - 2021. Mastology, 2021. http://dx.doi.org/10.29289/259453942021v31s1051.
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Introduction: Breast cancer (BC) is the most common type of cancer after non-melanoma skin tumors among Brazilian women, with 61.61 cases estimated for 100 thousand women in 2020. New biomarkers, such as miRNAs and selected proteins, are essential in personalized medicine. Objectives: To evaluate the expression and possible role of miR-146a-5p in subtypes of BC. Methods: miRNAs selection was performed using in silico analysis from the TCGA (The Cancer Genome Atlas) database. Data from the miRNAs expression of 1,085 patients were accessed and compared among BC subtypes. After normalization, the Bayesian Student t-test evaluated differential expression (DE) analysis via the limma R package. Lists with DE miRNAs were divided between up and down-regulated status (FC = ±2). A second approach was to submit the data obtained from BC samples´ mass spectrometry to IPA software to predict the activation/inhibition of upstream regulators in DE proteins lists in the tumor (T) versus contralateral tissue (CT). Results: A total of 206 upstream regulators were discovered at p <0.05; 12.6% of them were predicted with z-score values. In a TCGA analysis, miR-146a-5p was found up-regulated in triple-negative (TNBC) in comparison to other subtypes as a hormonal receptor (HR)+, HER2+, and non-TNBC (HR+ plus HER2+). The same was observed in TNBC cell lines by RT-qPCR. This miRNA was also predicted as an indirect regulator of CAT, LTF, CFH, and PGLYRP2 proteins in an IPA analysis. The proteomic analysis also demonstrated these molecules´ relation with cancer hallmarks such as invasion, inflammation, and immune response. Conclusions: The results suggest that miR-146a-5p deregulation has a role in BC, mainly in TNBC, via the regulation of essential proteins. A better understanding of these molecules in BC is critical to define new biomarkers.
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Teneva, Ivanka. "LIGHT-REPRESSED PROTEIN (LRP) AS A SUITABLE MOLECULAR MARKER FOR PHYLOGENETIC ANALYSES AND TAXONOMIC CLASSIFICATION WITHIN CYANOBACTERIA." In 18th International Multidisciplinary Scientific GeoConference SGEM2018. Stef92 Technology, 2018. http://dx.doi.org/10.5593/sgem2018/5.2/s20.075.
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Beaton, Nigel, Jagat Adhikari, Yuehan Feng, Roland Bruderer, Ron Tomlinson, Ivan Cornella-Taracido, and Lukas Reiter. "Abstract 298: Dissection of drug-protein interactions by HR-LiP-MS in target validation and lead optimization." In Proceedings: AACR Annual Meeting 2021; April 10-15, 2021 and May 17-21, 2021; Philadelphia, PA. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.am2021-298.
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Abreu, Maria M., and Linda Sealy. "Abstract 2861: The C/EBPbeta isoform, liver-inhibitory protein (LIP), induces autophagy in breast cancer cell lines." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-2861.
Full textReports on the topic "Ltp protein"
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Porat, Ron, Gregory T. McCollum, Amnon Lers, and Charles L. Guy. Identification and characterization of genes involved in the acquisition of chilling tolerance in citrus fruit. United States Department of Agriculture, December 2007. http://dx.doi.org/10.32747/2007.7587727.bard.
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Citrus, like many other tropical and subtropical fruit are sensitive to chilling temperatures. However, application of a pre-storage temperature conditioning (CD) treatment at 16°C for 7 d or of a hot water brushing (HWB) treatment at 60°C for 20 sec remarkably enhances chilling tolerance and reduces the development of chilling injuries (CI) upon storage at 5°C. In the current research, we proposed to identify and characterize grapefruit genes that are induced by CD, and may contribute to the acquisition of fruit chilling tolerance, by two different molecular approaches: cDNA array analysis and PCR cDNA subtraction. In addition, following the recent development and commercialization of the new Affymetrix Citrus Genome Array, we further performed genome-wide transcript profiling analysis following exposure to CD and chilling treatments. To conduct the cDNA array analysis, we constructed cDNA libraries from the peel tissue of CD- and HWB-treated grapefruit, and performed an EST sequencing project including sequencing of 3,456 cDNAs from each library. Based on the obtained sequence information, we chose 70 stress-responsive and chilling-related genes and spotted them on nylon membranes. Following hybridization the constructed cDNA arrays with RNA probes from control and CD-treated fruit and detailed confirmations by RT-PCR analysis, we found that six genes: lipid-transfer protein, metallothionein-like protein, catalase, GTP-binding protein, Lea5, and stress-responsive zinc finger protein, showed higher transcript levels in flavedo of conditioned than in non-conditioned fruit stored at 5 ᵒC. The transcript levels of another four genes: galactinol synthase, ACC oxidase, temperature-induced lipocalin, and chilling-inducible oxygenase, increased only in control untreated fruit but not in chilling-tolerant CD-treated fruit. By PCR cDNA subtraction analysis we identified 17 new chilling-responsive and HWB- and CD-induced genes. Overall, characterization of the expression patterns of these genes as well as of 11 more stress-related genes by RNA gel blot hybridizations revealed that the HWB treatment activated mainly the expression of stress-related genes(HSP19-I, HSP19-II, dehydrin, universal stress protein, EIN2, 1,3;4-β-D-glucanase, and SOD), whereas the CD treatment activated mainly the expression of lipid modification enzymes, including fatty acid disaturase2 (FAD2) and lipid transfer protein (LTP). Genome wide transcriptional profiling analysis using the newly developed Affymetrix Citrus GeneChip® microarray (including 30,171 citrus probe sets) revealed the identification of three different chilling-related regulons: 1,345 probe sets were significantly affected by chilling in both control and CD-treated fruits (chilling-response regulon), 509 probe sets were unique to the CD-treated fruits (chilling tolerance regulon), and 417 probe sets were unique to the chilling-sensitive control fruits (chilling stress regulon). Overall, exposure to chilling led to expression governed arrest of general cellular metabolic activity, including concretive down-regulation of cell wall, pathogen defense, photosynthesis, respiration, and protein, nucleic acid and secondary metabolism. On the other hand, chilling enhanced various adaptation processes, such as changes in the expression levels of transcripts related to membranes, lipid, sterol and carbohydrate metabolism, stress stimuli, hormone biosynthesis, and modifications in DNA binding and transcription factors.
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Sela, Shlomo, and Michael McClelland. Investigation of a new mechanism of desiccation-stress tolerance in Salmonella. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7598155.bard.
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Low-moisture foods (LMF) are increasingly involved in foodborne illness. While bacteria cannot grow in LMF due to the low water content, pathogens such as Salmonella can still survive in dry foods and pose health risks to consumer. We recently found that Salmonella secretes a proteinaceous compound during desiccation, which we identified as OsmY, an osmotic stress response protein of 177 amino acids. To elucidate the role of OsmY in conferring tolerance against desiccation and other stresses in Salmonella entericaserovarTyphimurium (STm), our specific objectives were: (1) Characterize the involvement of OsmY in desiccation tolerance; (2) Perform structure-function analysis of OsmY; (3) Study OsmY expression under various growth- and environmental conditions of relevance to agriculture; (4) Examine the involvement of OsmY in response to other stresses of relevance to agriculture; and (5) Elucidate regulatory pathways involved in controlling osmY expression. We demonstrated that an osmY-mutant strain is impaired in both desiccation tolerance (DT) and in long-term persistence during cold storage (LTP). Genetic complementation and addition of a recombinantOsmY (rOsmY) restored the mutant survival back to that of the wild type (wt). To analyze the function of specific domains we have generated a recombinantOsmY (rOsmY) protein. A dose-response DT study showed that rOsmY has the highest protection at a concentration of 0.5 nM. This effect was protein- specific as a comparable amount of bovine serum albumin, an unrelated protein, had a three-time lower protection level. Further characterization of OsmY revealed that the protein has a surfactant activity and is involved in swarming motility. OsmY was shown to facilitate biofilm formation during dehydration but not during bacterial growth under optimal growth conditions. This finding suggests that expression and secretion of OsmY under stress conditions was potentially associated with facilitating biofilm production. OsmY contains two conserved BON domains. To better understand the role of the BON sites in OsmY-mediated dehydration tolerance, we have generated two additional rOsmY constructs, lacking either BON1 or BON2 sites. BON1-minus (but not BON2) protein has decreased dehydration tolerance compared to intact rOsmY, suggesting that BON1 is required for maximal OsmY-mediated activity. Addition of BON1-peptide at concentration below 0.4 µM did not affect STm survival. Interestingly, a toxic effect of BON1 peptide was observed in concentration as low as 0.4 µM. Higher concentrations resulted in complete abrogation of the rOsmY effect, supporting the notion that BON-mediated interaction is essential for rOsmY activity. We performed extensive analysis of RNA expression of STm undergoing desiccation after exponential and stationary growth, identifying all categories of genes that are differentially expressed during this process. We also performed massively in-parallel screening of all genes in which mutation caused changes in fitness during drying, identifying over 400 such genes, which are now undergoing confirmation. As expected OsmY is one of these genes. In conclusion, this is the first study to identify that OsmY protein secreted during dehydration contributes to desiccation tolerance in Salmonella by facilitating dehydration- mediated biofilm formation. Expression of OsmY also enhances swarming motility, apparently through its surfactant activity. The BON1 domain is required for full OsmY activity, demonstrating a potential intervention to reduce pathogen survival in food processing. Expression and fitness screens have begun to elucidate the processes of desiccation, with the potential to uncover additional specific targets for efforts to mitigate pathogen survival in desiccation.
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Shomer, Ilan, Louise Wicker, Uzi Merin, and William L. Kerr. Interactions of Cloud Proteins, Pectins and Pectinesterases in Flocculation of Citrus Cloud. United States Department of Agriculture, February 2002. http://dx.doi.org/10.32747/2002.7580669.bard.
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The overall objective was to understand the cloud flocculation of citrus juice by characterization of the interactions between proteins and pectins, and to determine the role of PE isozymes in catalyzing this phenomenon. Specific objectives were to: 1. identify/characterize cloud-proteins in relation to their coagulable properties and affinity to pectins; 2. to determine structural changes of PME and other proteins induced by cation/pectin interactions; 3. localize cloud proteins, PME and bound protein/pectates in unheated and pasteurized juices; 4. to create "sensitized" pectins and determine their effect on clarification. The original objectives were not changed but the methods and approach were modified due to specific research requirements. Two i postulates were: 1. there is a specific interaction of cloud proteins with de-esterified regions of ! pectin and this contributes to cloud loss; 2. isozymes of pectin-methyl-esterase (PME) vary in efficiency to create sensitized pectins. The appearance of citrus fruit juice is an important quality factor and is determined by the color and turbidity that .are conferred by the suspended particles, i.e., by the cloud and its homogeneity. Under some circumstances the cloud tend to flocculate and the juice clarifies. The accepted approach to explain the clarification is based on pectin demethoxylation by PME that promotes formation of Ca-pectate. Therefore, the juice includes immediate heat-inactivation upon ~ squeezing. Protein coagulation also promotes cloud instability of citrus fruit extracts. However, the clarification mechanism is not fully understood. Information accumulated from several laboratories indicates that clarification is a more complex process than can be explained by a single mechanism. The increasing trend to consume natural-fresh juice emphasizing the importance of the knowledge to assure homogeneity of fresh juice. The research included complementary directions: Conditions that induce cloud-instability of natural- juice [IL]. Evaluate purification schemes of protein [USA]. Identifications of proteins, pectin and neutral sugars ([IL]; Structure of the cloud components using light and electron microscopy and immuno-labeling of PME, high-methoxyl-pectin (HMP) and low-methoxyl-pectin (LMP); Molecular weight of calcium sensitized pectins [US]; Evaluation of the products of PME activity [US]. Fractions and size distribution and cloud components [IL-US]. The optimal pH activity of PME is 7 and the flocculation pH of the cloud is 3-4. Thus, the c roles of PME, proteins and pectins in the cloud instability, were studied in pH ranges of 2- 7. The experiments led to establish firstly repeatable simulate conditions for cloud instability [IL]. Thermostable PME (TS-PE) known to induce cloud instability, but also thermolabile forms of PME (TL-PE) caused clarification, most likely due to the formation and dissolution of inactive :. PE-pectin complexes and displacement of a protective colloid from the cloud surface [US]. Furthermore, elimination of non-PME protein increases TS-PE activity, indicating that non-PME proteins moderate PME activity [US]. Other experiments Concomitantly with the study of the PME activity but promotes the association of cloud-proteins to pectin. Adjusting of the juice pH to f 7 retains the cloud stability and re-adjusting of the pH to 40% DE reacts to immuno-labeling in the cloud fragments, whereas
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Roser T. and J. Skelly. Emittance Measurements of 200 MeV Proton Beam in the LTB Transfer Line. Office of Scientific and Technical Information (OSTI), June 1992. http://dx.doi.org/10.2172/1131605.
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Harmon, David L., Israel Bruckental, Gerald B. Huntington, Yoav Aharoni, and Amichai Arieli. Influence of Small Intestinal Protein on Carbohydrate Assimilation in Beef and Dairy Cattle. United States Department of Agriculture, August 1995. http://dx.doi.org/10.32747/1995.7570572.bard.
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The long term goal of the proposed research, "Influence of small intestinal protein on carbohydrate assimilation and metabolism in beef and dairy cattle" was to define the limits of small intestinal starch digestion and clarify regulatory mechanisms involved in starch assimilation in cattle. It was hypothesized that dietary protein plays a critical role in the regulation of intestinal digestion; however, studies clearly identifying this role were lacking. The first two experiments quantified starch digestion (disappearance from the small intestine) in response to known increments in duodenal protein supply and found that the quantity of DM, OM and starch disappearing from the small intestine increased linearly (P <.01) with protein infusion. A follow-up experiment also demonstrated that casein infusion linearly increased pancreatic a-amylase concentration and secretion rate. The final experiment provided critical data on metabolic fates of glucose derived from intestinal starch digestion. These data demonstrated that increasing postruminal starch supply does increase the metabolism of glucose by visceral tissues: however, this increase is minor (20%) compared with the increase in portal production (70%). These changes can have a dramatic impact on the glucose economy of the animal and result in large increases in the amount of glucose reaching peripheral tissues.
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Weller, Joel, Harris Lewin, Micha Ron, and George Wiggans. Detection and Mapping of Genes Affecting Traits of Economic Importance in Dairy Cattle with the Aid of Molecular Genetic Markers. United States Department of Agriculture, December 1995. http://dx.doi.org/10.32747/1995.7613024.bard.
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Forty-seven poly-TG microsatellites were developed at the U of IL, and 11 genetic markers were developed at ARO, nine of which were poly-AGC microsatellites. Markers were typed on the reference families of CSIRO, Australia; GRANADA, Texas; and IRRF, Illinois, for chromosome assignment and linkage mapping. Nine North American al organizations contributed semen to the Dairy Bull DNA Repository (DBDR), which currently has 65,743 units from 3366 bulls. Semen was obtained for 31 out of 35 grandsires. Semen of 28 and 23 sons of two Israeli bulls was also collected. Eighteen grandsires were genotyped for 75 microsatellites. One thousand, three hundred and sixty-two sons with evaluation from 17 families were genotyped for 24 markers. Eleven thousand, six hundred and twenty sons genotypes were determined, of which 8,802 were informative. The genotype data was matched to the bulls' daughter yield deviations (DYD) for seven traits; milk, fat, and protein production; fat and protein percent; somatic cell concentration (SCS); and productive herd life. Seven loci had significant effects at p<0.05, but only two loci, TGLA263 and MGTG7, had significant effects at p<0.01, and the effect of TGLA263 on fat percentage was significant at p<0.0001. There was at least one significant effect for each of the seven traits analyzed.
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Clogher, L. A Precise Measurement of the Proton Elastic Form Factors for 1.75 <= Q**2 <= 8.83 (GeV/c)**2. Office of Scientific and Technical Information (OSTI), June 2004. http://dx.doi.org/10.2172/826980.
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Weller, Joel I., Harris A. Lewin, and Micha Ron. Determination of Allele Frequencies for Quantitative Trait Loci in Commercial Animal Populations. United States Department of Agriculture, February 2005. http://dx.doi.org/10.32747/2005.7586473.bard.
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Individual loci affecting economic traits in dairy cattle (ETL) have been detected via linkage to genetic markers by application of the granddaughter design in the US population and the daughter design in the Israeli population. From these analyses it is not possible to determine allelic frequencies in the population at large, or whether the same alleles are segregating in different families. We proposed to answer this question by application of the "modified granddaughter design", in which granddaughters with a common maternal grandsire are both genotyped and analyzed for the economic traits. The objectives of the proposal were: 1) to fine map three segregating ETL previously detected by a daughter design analysis of the Israeli dairy cattle population; 2) to determine the effects of ETL alleles in different families relative to the population mean; 3) for each ETL, to determine the number of alleles and allele frequencies. The ETL on Bostaurusautosome (BT A) 6 chiefly affecting protein concentration was localized to a 4 cM chromosomal segment centered on the microsatellite BM143 by the daughter design. The modified granddaughter design was applied to a single family. The frequency of the allele increasing protein percent was estimated at 0.63+0.06. The hypothesis of equal allelic frequencies was rejected at p<0.05. Segregation of this ETL in the Israeli population was confirmed. The genes IBSP, SPP1, and LAP3 located adjacent to BM143 in the whole genome cattle- human comparative map were used as anchors for the human genome sequence and bovine BAC clones. Fifteen genes within 2 cM upstream of BM143 were located in the orthologous syntenic groups on HSA4q22 and HSA4p15. Only a single gene, SLIT2, was located within 2 cM downstream of BM143 in the orthologous HSA4p15 region. The order of these genes, as derived from physical mapping of BAC end sequences, was identical to the order within the orthologous syntenic groups on HSA4: FAM13A1, HERC3. CEB1, FLJ20637, PP2C-like, ABCG2, PKD2. SPP, MEP, IBSP, LAP3, EG1. KIAA1276, HCAPG, MLR1, BM143, and SLIT2. Four hundred and twenty AI bulls with genetic evaluations were genotyped for 12 SNPs identified in 10 of these genes, and for BM143. Seven SNPs displayed highly significant linkage disequilibrium effects on protein percentage (P<0.000l) with the greatest effect for SPP1. None of SNP genotypes for two sires heterozygous for the ETL, and six sires homozygous for the ETL completely corresponded to the causative mutation. The expression of SPP 1 and ABCG2 in the mammary gland corresponded to the lactation curve, as determined by microarray and QPCR assays, but not in the liver. Anti-sense SPP1 transgenic mice displayed abnormal mammary gland differentiation and milk secretion. Thus SPP 1 is a prime candidate gene for this ETL. We confirmed that DGAT1 is the ETL segregating on BTA 14 that chiefly effects fat concentration, and that the polymorphism is due to a missense mutation in an exon. Four hundred Israeli Holstein bulls were genotyped for this polymorphism, and the change in allelic frequency over the last 20 years was monitored.
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Chen, Xiaole, Peng Wang, Yunquan Luo, Yi-Yu Lu, Wenjun Zhou, Mengdie Yang, Jian Chen, Zhi-Qiang Meng, and Shi-Bing Su. Therapeutic Efficacy Evaluation and Underlying Mechanisms Prediction of Jianpi Liqi Decoction for Hepatocellular Carcinoma. Science Repository, September 2021. http://dx.doi.org/10.31487/j.jso.2021.02.04.sup.
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Objective: The aim of this study was to assess the therapeutic effects of Jianpi Liqi decoction (JPLQD) in hepatocellular carcinoma (HCC) and explore its underlying mechanisms. Methods: The characteristics and outcomes of HCC patients with intermediate stage B who underwent sequential conventional transcatheter arterial chemoembolization (cTACE) and radiofrequency ablation (RFA) only or in conjunction with JPLQD were analysed retrospectively. The plasma proteins were screened using label-free quantitative proteomics analysis. The effective mechanisms of JPLQD were predicted through network pharmacology approach and partially verified by ELISA. Results: Clinical research demonstrated that the Karnofsky Performance Status (KPS), traditional Chinese medicine (TCM) syndrome scores, neutropenia and bilirubin, median progression-free survival (PFS), and median overall survival (OS) in HCC patients treated with JPLQD were superior to those in patients not treated with JPLQD (all P<0.05). The analysis of network pharmacology, combined with proteomics, suggested that 52 compounds targeted 80 potential targets, which were involved in the regulation of multiple signaling pathways, especially affecting the apoptosis-related pathways including TNF, p53, PI3K-AKT, and MAPK. Plasma IGFBP3 and CA2 were significantly up-regulated in HCC patients with sequential cTACE and RFA therapy treated with JPLQD than those in patients not treated with JPLQD (P<0.001). The AUC of the IGFBP3 and CA2 panel, estimated using ROC analysis for JPLQD efficacy evaluation, was 0.867. Conclusion: These data suggested that JPLQD improves the quality of life, prolongs the overall survival, protects liver function in HCC patients, and exhibits an anticancer activity against HCC. IGFBP3 and CA2 panels may be potential therapeutic targets and indicators in the efficacy evaluation for JPLQD treatment, and the effective mechanisms involved in the regulation of multiple signaling pathways, possibly affected the regulation of apoptosis.
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Rinkevich, Baruch, and Cynthia Hunter. Inland mariculture of reef corals amenable for the ornamental trade. United States Department of Agriculture, January 2006. http://dx.doi.org/10.32747/2006.7695880.bard.
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The worldwide market for ornamental saltwater invertebrates supplies the needs of millions of aquarium hobbyists, public exhibitions (i.e., zoos) universities and research institutions. With respect to reef building corals, it is estimated that more than half a million coral colonies/year from a total 93 genera, were exported globally during the period of 1985-1997. International value of retail sale of live coral trade alone is estimated as $78 million in 1997 (not including the illegally, widely smuggled material). The continuous, large-scale collection of marine organisms is responsible, in many places, for the destruction of coral reefs. The expected expansion of the trade further threatens these fragile habitats. While no true captive-bred corals are commercially available, our long-term goal is to develop ex situ inland farming of coral colonies that will circumvent the need for in situ collections and will provide domesticated specimens for the trade and for research. We simultaneously studied two model branching coral species, Stylophora pistillata (Pocilloporidae; in Israel) and Porites (Poritidae; in the US). The proposal included three specific aims: (a) To develop protocols for nubbins (small fragments, down to the size of a single polyp) usage in coral farming;(b) To address the significance of colony pattern formation to the coral trade; and (c) To develop the protocols of using nubbins in physiological and ecotoxicological assays (using oil dispersants, the expression of the stress protein HSP-70, household detergents, etc.). Ten scientific publications (published manuscripts, accepted for publications, submitted to scientific journals, in preparation), revealing results that were related to all three specific aims, originated from this BARD proposal. As a result of the work supported by the BARD, we have now, in hand, original and improved protocols for coral maintenance ex situ, proven expertise on manipulating coral colonies’ pattern formation and biological knowledge on island mariculture of reef corals (from Hawaii and from the Red Sea) amenable for the ornamental trade (for public and private aquaria use, for experimentation). At least one Israeli company (Red Sea Corals, Ltd., KibbutzSaar) is using our methodologies for further developing this new mariculture sector. We are now in the process of introducing the rationale and methodologies to Hawaiian private entities to expand dissemination of the research outcomes.