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Journal articles on the topic 'Lpt complex'

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Author: Grafiati

Published: 9 March 2023

Last updated: 10 March 2023

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1

Shin, Jeong Bin, Seong Min Lee, Myeongcheol Kim, Donghyuk Kim, Duk Young Jeon, and Kyung Cheol Choi. "Plasmonically Enhanced Optical Characteristics From Europium Organometallic Complex." IEEE Photonics Technology Letters 25, no. 23 (December 2013): 2342–45. http://dx.doi.org/10.1109/lpt.2013.2285257.

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2

Engin, D. "Complex Optical Low Coherence Reflectometry (OLCR) With Tunable Source." IEEE Photonics Technology Letters 16, no. 5 (May 2004): 1346–48. http://dx.doi.org/10.1109/lpt.2004.826103.

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3

Foster, Scott. "Complex Susceptibility of Saturated Erbium-Doped Fiber Lasers and Amplifiers." IEEE Photonics Technology Letters 19, no. 12 (June 2007): 895–97. http://dx.doi.org/10.1109/lpt.2007.896625.

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4

Adams, D. B., and C. K. Madsen. "Complex Optical Spectrum Analysis Using a Tunable Dispersive Optical Filter." IEEE Photonics Technology Letters 20, no. 2 (January 2008): 90–92. http://dx.doi.org/10.1109/lpt.2007.911507.

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5

Teng, J. H., L. F. Chong, J. R. Dong, S. J. Chua, S. S. Ang, Y. J. Wang, and E. L. Lim. "Complex-Coupled DFB Laser Using a Buried SiO$_{2}$ Grating." IEEE Photonics Technology Letters 20, no. 4 (February 2008): 231–33. http://dx.doi.org/10.1109/lpt.2007.913262.

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6

Li, Wei, Wen Ting Wang, Wen Hui Sun, Jian Guo Liu, and Ning Hua Zhu. "Microwave Photonic Notch Filter With Complex Coefficient Based on DDMZM." IEEE Photonics Technology Letters 26, no. 18 (September 15, 2014): 1859–62. http://dx.doi.org/10.1109/lpt.2014.2320459.

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7

Greenberg, M., and M. Orenstein. "Filterless "Add" multiplexer based on novel complex gratings assisted coupler." IEEE Photonics Technology Letters 17, no. 7 (July 2005): 1450–52. http://dx.doi.org/10.1109/lpt.2005.848560.

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8

Lysak, V. V., I. A. Sukhoivanov, O. V. Shulika, I. M. Safonov, and Y. T. Lee. "Carrier tunneling in complex asymmetrical multiple-quantum-well semiconductor optical amplifiers." IEEE Photonics Technology Letters 18, no. 12 (June 2006): 1362–64. http://dx.doi.org/10.1109/lpt.2006.877334.

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9

Loayssa, A., J. Capmany, M. Sagues, and J. Mora. "Demonstration of incoherent microwave photonic filters with all-optical complex coefficients." IEEE Photonics Technology Letters 18, no. 16 (August 2006): 1744–46. http://dx.doi.org/10.1109/lpt.2006.879535.

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10

Villafranca, A., J. Lasobras, R. Alonso, F. M. Lopez, and I. Garces. "Complex Spectrum Analysis of Modulated Optical Signals Using Stimulated Brillouin Scattering." IEEE Photonics Technology Letters 20, no. 23 (December 2008): 1938–40. http://dx.doi.org/10.1109/lpt.2008.2005423.

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11

Bazargani, Hamed Pishvai, Jean-Baptiste Quelene, Patrick Dumais, Antonio Malacarne, Matteo Clerici, Roberto Morandotti, Claire L. Callender, and Jose Azana. "On-Chip, Single-Shot Characterization of GHz-Rate Complex Optical Signals." IEEE Photonics Technology Letters 26, no. 23 (December 1, 2014): 2345–48. http://dx.doi.org/10.1109/lpt.2014.2354636.

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12

Che, Di, Qian Hu, Feng Yuan, Qi Yang, and William Shieh. "Enabling Complex Modulation of Directly Modulated Signals Using Laser Frequency Chirp." IEEE Photonics Technology Letters 27, no. 22 (November 15, 2015): 2407–10. http://dx.doi.org/10.1109/lpt.2015.2467171.

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13

Wu, Di, R. Ohnishi, R. Uemura, T. Yamaguchi, and S. Ohnuki. "Finite-Difference Complex-Frequency-Domain Method for Optical and Plasmonic Analyses." IEEE Photonics Technology Letters 30, no. 11 (June 1, 2018): 1024–27. http://dx.doi.org/10.1109/lpt.2018.2828167.

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14

Sciara, Stefania, Piotr Roztocki, Cristina Rimoldi, Mario Chemnitz, Bennet Fischer, Luis Romero Cortes, William J. Munro, et al. "Generation and Processing of Complex Photon States With Quantum Frequency Combs." IEEE Photonics Technology Letters 31, no. 23 (December 1, 2019): 1862–65. http://dx.doi.org/10.1109/lpt.2019.2944564.

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15

Alweimine, A. Ould Baba, O. Bamaarouf, A. Rachadi, and H. Ez-Zahraouy. "Implementing beneficial prioritization of traffic flow in complex networks." International Journal of Modern Physics B 32, no. 24 (September 13, 2018): 1850273. http://dx.doi.org/10.1142/s0217979218502739.

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Despite the reality of traffic prioritization in real networks as the internet, this has almost been ignored in designed routing protocols. In this work, we propose a priority policy where packets are prioritized according to their destination. This procedure is applied to a fraction f of nodes; packets are classified as High Priority if their destinations are among the fraction f, otherwise the packets are treated as Low Priority. Using numerical simulation we found that the prioritization of nodes with high degree (hubs) is always more efficient than the prioritization of nodes with small degree or the random prioritization of nodes. Moreover, we observed three regimes based on the prioritized fraction f in the network: the first one is characterized by an improvement of the High Priority Traffic (HPT) flow without any degradation of the flow of the Low Priority Traffic (LPT). In the second regime, the HPT gains some performance at the expense of a loss of the performance in the LPT flow. While in the last regime the LPT experiences a low performance without any noticeable improvement in HPT compared to the normal flow.

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16

Lee, Seong Min, Woo Hyun Kim, and Kyung Cheol Choi. "Localized Surface Plasmon Coupled Photoluminescence of Divalent Europium Complex With Silver Nanoparticles." IEEE Photonics Technology Letters 23, no. 19 (October 2011): 1415–17. http://dx.doi.org/10.1109/lpt.2011.2162626.

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17

Zhang, Yamei, and Shilong Pan. "Complex Coefficient Microwave Photonic Filter Using a Polarization-Modulator-Based Phase Shifter." IEEE Photonics Technology Letters 25, no. 2 (January 2013): 187–89. http://dx.doi.org/10.1109/lpt.2012.2227692.

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18

Sagues, M., A. Loayssa, and J. Capmany. "Multitap Complex-Coefficient Incoherent Microwave Photonic Filters Based on Stimulated Brillouin Scattering." IEEE Photonics Technology Letters 19, no. 16 (August 2007): 1194–96. http://dx.doi.org/10.1109/lpt.2007.901493.

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19

Liang, Hu, Zhenhong Wang, Ruijing He, Yange Liu, Hongye Li, Lifa Ni, and Zhi Wang. "Evolution of Complex Pulse-Bunches in a Bound-State Soliton Fiber Laser." IEEE Photonics Technology Letters 30, no. 16 (August 15, 2018): 1475–78. http://dx.doi.org/10.1109/lpt.2018.2856863.

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20

Jandieri, Vakhtang, Paolo Baccarelli, Guido Valerio, Kiyotoshi Yasumoto, and Giuseppe Schettini. "Modal Propagation in Periodic Chains of Circular Rods: Real and Complex Solutions." IEEE Photonics Technology Letters 32, no. 17 (September 1, 2020): 1053–56. http://dx.doi.org/10.1109/lpt.2020.3011321.

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21

Xiang, Yating, Yizhao Chen, Junda Chen, Li Wang, Ting Jiang, and Ming Tang. "Phase Retrieval of Complex OFDM Signal by Solving Temporal Transport-of-Intensity Equation." IEEE Photonics Technology Letters 33, no. 18 (September 15, 2021): 1006–9. http://dx.doi.org/10.1109/lpt.2021.3085403.

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22

Gupta, J. A., P. J. Barrios, J. Lapointe, G. C. Aers, C. Storey, and P. Waldron. "Modal Gain of 2.4-$\mu$m InGaAsSb–AlGaAsSb Complex-Coupled Distributed-Feedback Lasers." IEEE Photonics Technology Letters 21, no. 20 (October 2009): 1532–34. http://dx.doi.org/10.1109/lpt.2009.2029244.

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23

Singh, G., Eng Leong Tan, and Zhi Ning Chen. "Efficient Complex Envelope ADI-FDTD Method for the Analysis of Anisotropic Photonic Crystals." IEEE Photonics Technology Letters 23, no. 12 (June 2011): 801–3. http://dx.doi.org/10.1109/lpt.2011.2138123.

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24

Palavicini, C., G. Campuzano, B. Thedrez, Y. Jaouen, and P. Gallion. "Analysis of optical-injected distributed feedback lasers using complex optical low-coherence reflectometry." IEEE Photonics Technology Letters 15, no. 12 (December 2003): 1683–85. http://dx.doi.org/10.1109/lpt.2003.819734.

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25

Gui-Rong Zhou, Ning-Ning Feng, and Xun Li. "A digital filter approach for complex frequency-shifted perfectly matched layer in semivectorial FDTD." IEEE Photonics Technology Letters 15, no. 11 (November 2003): 1552–54. http://dx.doi.org/10.1109/lpt.2003.818650.

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26

Liang, C. J., Wallace C. H. Choy, and ChunHui Huang. "The Electroluminescent Decay Mechanism of Rare-Earth Ions in OLEDs Based on a Terbium Complex." IEEE Photonics Technology Letters 19, no. 15 (August 2007): 1178–80. http://dx.doi.org/10.1109/lpt.2007.901599.

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27

Yan, Yu, and Jianping Yao. "A Tunable Photonic Microwave Filter With a Complex Coefficient Using an Optical RF Phase Shifter." IEEE Photonics Technology Letters 19, no. 19 (October 2007): 1472–74. http://dx.doi.org/10.1109/lpt.2007.903753.

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28

Zhang, Qian, Yubin Hou, Shuxian Qi, and Pu Wang. "Low-Noise Single-Frequency 1.5- $\mu$ m Fiber Laser With a Complex Optical-Feedback Loop." IEEE Photonics Technology Letters 29, no. 2 (January 15, 2017): 193–96. http://dx.doi.org/10.1109/lpt.2016.2632747.

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29

Zhou, Quanbin, Mingsheng Xu, Qixin Li, and Hong Wang. "Improved Efficiency of GaN-Based Green LED by a Nano-Micro Complex Patterned Sapphire Substrate." IEEE Photonics Technology Letters 29, no. 12 (June 15, 2017): 983–86. http://dx.doi.org/10.1109/lpt.2017.2700854.

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30

Ferndandez-Diaz, J. M., A. Guinea, and S. L. Palacios. "Solution of nonlinear wave equations of the complex quintic Ginzburg-Landau and nonlinear Schrodinger type." IEEE Photonics Technology Letters 14, no. 6 (June 2002): 807–9. http://dx.doi.org/10.1109/lpt.2002.1003100.

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31

Mahnkopf, S., M. Kamp, M. Arlt, R. Marz, F. Lelarge, G. H. Duan, and A. Forchel. "Widely Tunable Complex-Coupled Distributed Feedback Laser With Photonic Crystal Mirrors and Integrated Optical Amplifier." IEEE Photonics Technology Letters 16, no. 3 (March 2004): 729–31. http://dx.doi.org/10.1109/lpt.2004.823765.

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32

Dong, H., P. Shum, Y. D. Gong, M. Yan, J. Q. Zhou, and C. Q. Wu. "Virtual Generalized Mueller Matrix Method for Measurement of Complex Polarization-Mode Dispersion Vector in Optical Fibers." IEEE Photonics Technology Letters 19, no. 1 (January 2007): 27–29. http://dx.doi.org/10.1109/lpt.2006.887888.

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33

Xiaoxu Deng, Zhuangqi Cao, Qishun Shen, and Wen Yuan. "Measurement of electrically induced change in complex refractive index of nonlinear polymer by improved attenuated-total-reflection technique." IEEE Photonics Technology Letters 15, no. 12 (December 2003): 1755–57. http://dx.doi.org/10.1109/lpt.2003.819744.

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34

Vetterli, Stefan U., Katja Zerbe, Maik Müller, Matthias Urfer, Milon Mondal, Shuang-Yan Wang, Kerstin Moehle, et al. "Thanatin targets the intermembrane protein complex required for lipopolysaccharide transport inEscherichia coli." Science Advances 4, no. 11 (November 2018): eaau2634. http://dx.doi.org/10.1126/sciadv.aau2634.

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With the increasing resistance of many Gram-negative bacteria to existing classes of antibiotics, identifying new paradigms in antimicrobial discovery is an important research priority. Of special interest are the proteins required for the biogenesis of the asymmetric Gram-negative bacterial outer membrane (OM). Seven Lpt proteins (LptA to LptG) associate in most Gram-negative bacteria to form a macromolecular complex spanning the entire envelope, which transports lipopolysaccharide (LPS) molecules from their site of assembly at the inner membrane to the cell surface, powered by adenosine 5′-triphosphate hydrolysis in the cytoplasm. The periplasmic protein LptA comprises the protein bridge across the periplasm, which connects LptB2FGC at the inner membrane to LptD/E anchored in the OM. We show here that the naturally occurring, insect-derived antimicrobial peptide thanatin targets LptA and LptD in the network of periplasmic protein-protein interactions required to assemble the Lpt complex, leading to the inhibition of LPS transport and OM biogenesis inEscherichia coli.

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35

Denis, Kevin, Johan Philips, Herman Bruyninckx, and Eric Demeester. "Clothoidal Local Path Template for Intention Estimation by Assistive Mobile Robots." Proceedings of the International Conference on Automated Planning and Scheduling 29 (May 25, 2021): 689–97. http://dx.doi.org/10.1609/icaps.v29i1.3537.

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This work proposes and evaluates various improvements to a circular local path template (LPT) that we have designed in the past to estimate driver intents and to provide navigation assistance to wheelchair drivers. This LPT may also be useful in other mobile robotics applications such as for intuitive teleoperated control, for fast collision checking in path planning or for obstacle avoidance algorithms. The LPT consists of a large but fixed set of paths in the mobile robot’s local neighborhood. Based on an efficient look-up table, the LPT paths’ lengths are adjusted such that they are collisionfree. However, experiments have shown that in dense environments insufficient circular paths are found, which impedes correct intention estimation and thus navigation assistance. In this work, the use of clothoidal paths rather than circular paths is evaluated. This substantially improves the capability to find complex paths in dense areas. Furthermore, we adapt the LPT to deal with dynamic obstacles of random shape using motion prediction estimates of these objects.

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36

Chih-Wei Hu, Feng-Ming Lee, Kun-Fu Huang, Meng-Chyi Wu, Chia-Lung Tsai, Yin-Hsun Huang, and C. C. Lin. "Uncooled 1.3-/spl mu/m complex-coupled DFB BH laser diodes with the Fe-doped InGaAsP-InP hybrid current-blocking grating." IEEE Photonics Technology Letters 18, no. 14 (July 2006): 1551–53. http://dx.doi.org/10.1109/lpt.2006.878154.

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37

Xiang, Quanju, Haiyan Wang, Zhongshan Wang, Yizheng Zhang, and Changjiang Dong. "Characterization of lipopolysaccharide transport protein complex." Open Life Sciences 9, no. 2 (February 1, 2014): 131–38. http://dx.doi.org/10.2478/s11535-013-0250-5.

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AbstractLipopolysaccharide (LPS) is an essential component of the outer membranes (OM) of most Gram-negative bacteria, which plays a crucial role in protection of the bacteria from toxic compounds and harsh conditions. The LPS is biosynthesized at the cytoplasmic side of inner membrane (IM), and then transported across the aqueous periplasmic compartment and assembled correctly at the outer membrane. This process is accomplished by seven LPS transport proteins (LptA-G), but the transport mechanism remains poorly understood. Here, we present findings by pull down assays in which the periplasmic component LptA interacts with both the IM complex LptBFGC and the OM complex LptDE in vitro, but not with complex LptBFG. Using purified Lpt proteins, we have successfully reconstituted the seven transport proteins as a complex in vitro. In addition, the LptC may play an essential role in regulating the conformation of LptBFG to secure the lipopolysaccharide from the inner membrane. Our results contribute to the understanding of lipopolysaccharide transport mechanism and will provide a platform to study the detailed mechanism of the LPS transport in vitro.

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38

Kotera, Hiroaki. "Double Ring Model for Foveated Imaging." Color and Imaging Conference 2020, no. 28 (November 4, 2020): 336–41. http://dx.doi.org/10.2352/issn.2169-2629.2020.28.53.

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Human visual system has a space-variant resolution nature. In the retinal receptive field, the resolution is not uniform but sampled finest in the central fovea and coarser in the peripheral. This variable resolution mapping function is born by the cerebral primary visual cortex V1. It has a clear visual field map of spatial information, and this spatial mapping structure is called Retinotopy. The forward mapping to visual cortex from retina is characterized with complex LPT (Log-PolarTransform) by Schwartz. The retinal receptive field image is reconstructed by inverse projection LPT-1 from V1. This reconstructed process is called F oveated I maging. Since the spatial information is concentrated in the center of the visual field, the Foveated Imaging is applied to image compression, pattern recognition, robot vision, and/or computer vision. The retinal receptive field image is suitable for material appearance expression with natural blurring due to peripheral vision.<br/> However, the complexity of the inverse transform LPT-1 was a bottleneck. This paper proposes a Double- Ring-structured novel Foveated Imaging method using positive and negative Gaussian blur masks without using the inverse transform LPT-1 of Schwartz theory and reports the evaluation of reproduction errors.

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39

Martorana, Alessandra M., Mattia Benedet, Elisa A. Maccagni, Paola Sperandeo, Riccardo Villa, Gianni Dehò, and Alessandra Polissi. "Functional Interaction between the Cytoplasmic ABC Protein LptB and the Inner Membrane LptC Protein, Components of the Lipopolysaccharide Transport Machinery in Escherichia coli." Journal of Bacteriology 198, no. 16 (May 31, 2016): 2192–203. http://dx.doi.org/10.1128/jb.00329-16.

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ABSTRACTThe assembly of lipopolysaccharide (LPS) in the outer leaflet of the outer membrane (OM) requires the transenvelope Lpt (lipopolysaccharide transport) complex, made inEscherichia coliof seven essential proteins located in the inner membrane (IM) (LptBCFG), periplasm (LptA), and OM (LptDE). At the IM, LptBFG constitute an unusual ATP binding cassette (ABC) transporter, composed by the transmembrane LptFG proteins and the cytoplasmic LptB ATPase, which is thought to extract LPS from the IM and to provide the energy for its export across the periplasm to the cell surface. LptC is a small IM bitopic protein that binds to LptBFG and recruits LptA via its N- and C-terminal regions, and its role in LPS export is not completely understood. Here, we show that the expression level oflptBis a critical factor for suppressing lethality of deletions in the C-terminal region of LptC and the functioning of a hybrid Lpt machinery that carriesPa-LptC, the highly divergent LptC orthologue fromPseudomonas aeruginosa. We found that LptB overexpression stabilizes C-terminally truncated LptC mutant proteins, thereby allowing the formation of a sufficient amount of stable IM complexes to support growth. Moreover, the LptB level seems also critical for the assembly of IM complexes carryingPa-LptC which is otherwise defective in interactions with theE. coliLptFG components. Overall, our data suggest that LptB and LptC functionally interact and support a model whereby LptB plays a key role in the assembly of the Lpt machinery.IMPORTANCEThe asymmetric outer membrane (OM) of Gram-negative bacteria contains in its outer leaflet an unusual glycolipid, the lipopolysaccharide (LPS). LPS largely contributes to the peculiar permeability barrier properties of the OM that prevent the entry of many antibiotics, thus making Gram-negative pathogens difficult to treat. InEscherichia colithe LPS transporter (the Lpt machine) is made of seven essential proteins (LptABCDEFG) that form a transenvelope complex. Here, we show that increased expression of the membrane-associated ABC protein LptB can suppress defects of LptC, which participates in the formation of the periplasmic bridge. This reveals functional interactions between these two components and supports a role of LptB in the assembly of the Lpt machine.

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40

Villa, R., A. M. Martorana, S. Okuda, L. J. Gourlay, M. Nardini, P. Sperandeo, G. Deho, M. Bolognesi, D. Kahne, and A. Polissi. "The Escherichia coli Lpt Transenvelope Protein Complex for Lipopolysaccharide Export Is Assembled via Conserved Structurally Homologous Domains." Journal of Bacteriology 195, no. 5 (January 4, 2013): 1100–1108. http://dx.doi.org/10.1128/jb.02057-12.

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41

Іваницька, Марія. "Translator’s Personality Shaping: Competence Approach." PSYCHOLINGUISTICS 26, no. 2 (November 12, 2019): 135–56. http://dx.doi.org/10.31470/2309-1797-2019-26-2-135-156.

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The aim of the article is to highlight the role of psycholinguistic factors in shaping the linguistic personality of the translator and in developing the translator’s professional competence. The research method is based on the notions of the linguistic personality of the translator (LPT) and comprehensive professional competence of the translator (CPCT). LPT is defined as the sum total of language abilities, skills, and language behaviour of a bilingual individual, determined inter alia, by socio-cultural and psycho-cognitive conditions of formation/development of his or her personality. CPCT is treated as a complex of interrelated subcompetences that includes such components: abilities, knowledge, language behaviour and skills. The procedure of research proceeds from the postulate that LPT becomes apparent in translation decisions, style, language priorities, forms of speech activity, aims and guidelines of the translator. The development possibilities of LPT and CPCT are studied on the basis of such empirical methods: a) observation of novice translators’ work with the non-standard translation commissions that presuppose creativity, research and text-creating competences; b) psycholinguistic analysis of translation decisions; c) identification of the deviations that occur in understanding of the translator’s task. Findings. The paper demonstrates the development possibilities of LPT and CPCT by means of orienting the academic process to the purposeful acquisition of all the subcompetences: from language and cross-cultural ones through translator, research and technological ones even to socio-communicative and personality ones, i.e. by orienting academic aims to the formation of CPCT. It presents the work on a German-Ukrainian translation textbook that is based on the theory of functional translation by Ch. Nord and is directed at fostering those academic aims, which progressively lead to the shaping of CPCT. The paper analyzes the results of the textbook’s approbation on the basis of exercises offered in the textbook. Conclusions. It is proven that the progression of academic aims is an important aspect of translators’ training. These aims presuppose gradual development of all the translator’s subcompetences, personality subcompetence, in particular, and take into consideration all four components of CPCT that are equally relevant for the successful performance of contemporary translators on the labour market.

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42

ORDÁS, B., L. SERRANO, A. ORDÁS, A. BUTRÓN, and P. REVILLA. "Transition between vegetative phases in maize: genetic effects and variances and associated markers." Journal of Agricultural Science 147, no. 5 (June 3, 2009): 547–54. http://dx.doi.org/10.1017/s0021859609990025.

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SUMMARYTransition from juvenile to adult vegetative phase in maize is associated with pest and disease resistance. However, reports are not consistent on the significance and relative importance of additive or dominance genetic effects and variances. The purpose of the present research was to elucidate the genetic effects and variances and to identify molecular markers associated with phase transition. Three cycles of divergent selection were carried out in a maize synthetic accelerating and delaying phase transition. Three and four inbred lines were released from the third cycles of late phase transition (LPT) and early phase transition (EPT), respectively. Generation mean analyses were performed from two LPT×EPT crosses in order to calculate genetic effects and variances. Markers associated with vegetative phase transition were identified by contrasting simple sequence repeat (SSR) alleles between LPT and EPT inbreds and selection cycles, and by testing whether drift could explain the allelic changes observed in the respective third cycles of selection. Juvenile traits are mainly regulated by additive genetic effects and variances, whereas adult traits have a complex regulation involving dominance and epistatic effects. Based on error variances, EPT improves phenotypic stability. The SSRs phi028, phi112 and umc1725 were associated with selection for phase transition, suggesting that these genome regions are involved in the regulation of vegetative phase transition of maize, although none of the genes previously associated with phase transition has been located in those regions.

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43

Ettore D’Ortona, Nicolino, Gabriella Marcarelli, and Giuseppe Melisi. "Loss portfolio transfer treaties within Solvency II capital system: a reinsurer’s point of view." Insurance Markets and Companies 11, no. 1 (March 26, 2020): 1–10. http://dx.doi.org/10.21511/ins.11(1).2020.01.

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Loss portfolio transfer (LPT) is a reinsurance treaty in which an insurer cedes the policies that have already incurred losses to a reinsurer. This operation can be carried out by an insurance company in order to reduce reserving risk and consequently reduce its capital requirement calculated, according to Solvency II. From the viewpoint of the reinsurance company, being a very complex operation, importance must be given to the methodology used to determine the price of the treaty.Following the collective risk approach, the paper examines the risk profiles and the reinsurance pricing of LPT treaties, taking into account the insurance capital requirements established by European law. For this purpose, it is essential to calculate the capital need for the risk deriving from the LPT transaction. In the case analyzed, this requirement is calculated under Solvency II legislation, considering the measure of variability determined via simulation. This quantification was also carried out for different levels of the cost of capital rate, providing a range of possible loadings to be applied to the premium. In the case of the Cost of Capital (CoC) approach, the results obtained provide a lower level of premium compared to the percentile-based method with a range between 2.69% and 1.88%. Besides, the CoC approach also provides the advantage of having an explicit parameter, the CoC rate whose specific level can be chosen by the reinsurance company based on the risk appetite.

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44

Kielb, Robert, Jack Barter, Olga Chernycheva, and Torsten Fransson. "Flutter of Low Pressure Turbine Blades With Cyclic Symmetric Modes: A Preliminary Design Method." Journal of Turbomachinery 126, no. 2 (April 1, 2004): 306–9. http://dx.doi.org/10.1115/1.1650380.

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A current preliminary design method for flutter of low pressure turbine blades and vanes only requires knowledge of the reduced frequency and mode shape (real). However, many low pressure turbine (LPT) blade designs include a tip shroud that mechanically connects the blades together in a structure exhibiting cyclic symmetry. A proper vibration analysis produces a frequency and complex mode shape that represents two real modes phase shifted by 90 deg. This paper describes an extension to the current design method to consider these complex mode shapes. As in the current method, baseline unsteady aerodynamic analyses must be performed for the three fundamental motions, two translations and a rotation. Unlike the current method work matrices must be saved for a range of reduced frequencies and interblade phase angles. These work matrices are used to generate the total work for the complex mode shape. Since it still only requires knowledge of the reduced frequency and mode shape (complex), this new method is still very quick and easy to use. Theory and an example application are presented.

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Riccardi, Nicholas, Chris Rorden, Julius Fridriksson, and Rutvik H. Desai. "Canonical Sentence Processing and the Inferior Frontal Cortex: Is There a Connection?" Neurobiology of Language 3, no. 2 (2022): 318–44. http://dx.doi.org/10.1162/nol_a_00067.

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Abstract The role of left inferior frontal cortex (LIFC) in canonical sentence comprehension is controversial. Many studies have found involvement of LIFC in sentence production or complex sentence comprehension, but negative or mixed results are often found in comprehension of simple or canonical sentences. We used voxel-, region-, and connectivity-based lesion symptom mapping (VLSM, RLSM, CLSM) in left-hemisphere chronic stroke survivors to investigate canonical sentence comprehension while controlling for lexical-semantic, executive, and phonological processes. We investigated how damage and disrupted white matter connectivity of LIFC and two other language-related regions, the left anterior temporal lobe (LATL) and posterior temporal-inferior parietal area (LpT-iP), affected sentence comprehension. VLSM and RLSM revealed that LIFC damage was not associated with canonical sentence comprehension measured by a sensibility judgment task. LIFC damage was associated instead with impairments in a lexical semantic similarity judgment task with high semantic/executive demands. Damage to the LpT-iP, specifically posterior middle temporal gyrus (pMTG), predicted worse sentence comprehension after controlling for visual lexical access, semantic knowledge, and auditory-verbal short-term memory (STM), but not auditory single-word comprehension, suggesting pMTG is vital for auditory language comprehension. CLSM revealed that disruption of left-lateralized white-matter connections from LIFC to LATL and LpT-iP was associated with worse sentence comprehension, controlling for performance in tasks related to lexical access, auditory word comprehension, and auditory-verbal STM. However, the LIFC connections were accounted for by the lexical semantic similarity judgment task, which had high semantic/executive demands. This suggests that LIFC connectivity is relevant to canonical sentence comprehension when task-related semantic/executive demands are high.

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Haldeman, C. W., M. L. Krumanaker, and M. G. Dunn. "Influence of Clocking and Vane/Blade Spacing on the Unsteady Surface Pressure Loading for a Modern Stage and One-Half Transonic Turbine." Journal of Turbomachinery 125, no. 4 (October 1, 2003): 743–53. http://dx.doi.org/10.1115/1.1625398.

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This paper describes pressure measurements obtained for a modern one and one-half stage turbine. As part of the experimental effort, the position of the high-pressure turbine (HPT) vane was clocked relative to the downstream low-pressure turbine (LPT) vane to determine the influence of vane clocking on both the steady and unsteady pressure loadings on the LPT vane and the HPT blade. In addition, the axial location of the HPT vane relative to the HPT blade was changed to investigate the combined influence of vane/blade spacing and clocking on the unsteady pressure loading. Time-averaged and time-accurate surface-pressure results are presented for several spanwise locations on the vanes and blade. Results were obtained at four different HPT vane-clocking positions and at two different vane/blade axial spacings for three (of the four) clocking positions. For time-averaged results, the effect of clocking is small on the HPT blade and vane. The influence of clocking on the transition ducts and the LPT vane is slightly greater (on the order of ±1%). Reduced HPT vane/blade spacing has a larger effect than clocking on the HPT vanes and blades ±3% depending upon the particular surface. Examining the data at blade passing and the first fundamental frequency, the effect of spacing does not produce a dramatic influence on the relative changes that occur between clocking positions. The results demonstrate that clocking and spacing effects on the surface pressure loading are very complex and may introduce problems if the results of measurements or analysis made at one span or location in the machine are extrapolated to other sections.

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Zraly, Claudia B., Abdul Zakkar, John Hertenstein Perez, Jeffrey Ng, Kevin P. White, Matthew Slattery, and Andrew K. Dingwall. "The Drosophila MLR COMPASS complex is essential for programming cis-regulatory information and maintaining epigenetic memory during development." Nucleic Acids Research 48, no. 7 (February 13, 2020): 3476–95. http://dx.doi.org/10.1093/nar/gkaa082.

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Abstract The MLR COMPASS complex monomethylates H3K4 that serves to epigenetically mark transcriptional enhancers to drive proper gene expression during animal development. Chromatin enrichment analyses of the Drosophila MLR complex reveals dynamic association with promoters and enhancers in embryos with late stage enrichments biased toward both active and poised enhancers. RNAi depletion of the Cmi (also known as Lpt) subunit that contains the chromatin binding PHD finger domains attenuates enhancer functions, but unexpectedly results in inappropriate enhancer activation during stages when hormone responsive enhancers are poised, revealing critical epigenetic roles involved in both the activation and repression of enhancers depending on developmental context. Cmi is necessary for robust H3K4 monomethylation and H3K27 acetylation that mark active enhancers, but not for the chromatin binding of Trr, the MLR methyltransferase. Our data reveal two likely major regulatory modes of MLR function, contributions to enhancer commissioning in early embryogenesis and bookmarking enhancers to enable rapid transcriptional re-activation at subsequent developmental stages.

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Simpson, Brent W., Janine M. May, David J. Sherman, Daniel Kahne, and Natividad Ruiz. "Lipopolysaccharide transport to the cell surface: biosynthesis and extraction from the inner membrane." Philosophical Transactions of the Royal Society B: Biological Sciences 370, no. 1679 (October 5, 2015): 20150029. http://dx.doi.org/10.1098/rstb.2015.0029.

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The cell surface of most Gram-negative bacteria is covered with lipopolysaccharide (LPS). The network of charges and sugars provided by the dense packing of LPS molecules in the outer leaflet of the outer membrane interferes with the entry of hydrophobic compounds into the cell, including many antibiotics. In addition, LPS can be recognized by the immune system and plays a crucial role in many interactions between bacteria and their animal hosts. LPS is synthesized in the inner membrane of Gram-negative bacteria, so it must be transported across their cell envelope to assemble at the cell surface. Over the past two decades, much of the research on LPS biogenesis has focused on the discovery and understanding of Lpt, a multi-protein complex that spans the cell envelope and functions to transport LPS from the inner membrane to the outer membrane. This paper focuses on the early steps of the transport of LPS by the Lpt machinery: the extraction of LPS from the inner membrane. The accompanying paper (May JM, Sherman DJ, Simpson BW, Ruiz N, Kahne D. 2015 Phil. Trans. R. Soc. B 370 , 20150027. ( doi:10.1098/rstb.2015.0027 )) describes the subsequent steps as LPS travels through the periplasm and the outer membrane to its final destination at the cell surface.

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Bryant, Jack A., Ian T. Cadby, Zhi-Soon Chong, Gabriela Boelter, Yanina R. Sevastsyanovich, Faye C. Morris, Adam F. Cunningham, et al. "Structure-Function Characterization of the Conserved Regulatory Mechanism of the Escherichia coli M48 Metalloprotease BepA." Journal of Bacteriology 203, no. 2 (October 26, 2020): e00434-20. http://dx.doi.org/10.1128/jb.00434-20.

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ABSTRACTThe asymmetric Gram-negative outer membrane (OM) is the first line of defense for bacteria against environmental insults and attack by antimicrobials. The key component of the OM is lipopolysaccharide, which is transported to the surface by the essential lipopolysaccharide transport (Lpt) system. Correct folding of the Lpt system component LptD is regulated by a periplasmic metalloprotease, BepA. Here, we present the crystal structure of BepA from Escherichia coli, solved to a resolution of 2.18 Å, in which the M48 protease active site is occluded by an active-site plug. Informed by our structure, we demonstrate that free movement of the active-site plug is essential for BepA function, suggesting that the protein is autoregulated by the active-site plug, which is conserved throughout the M48 metalloprotease family. Targeted mutagenesis of conserved residues reveals that the negative pocket and the tetratricopeptide repeat (TPR) cavity are required for function and degradation of the BAM complex component BamA under conditions of stress. Last, we show that loss of BepA causes disruption of OM lipid asymmetry, leading to surface exposed phospholipid.IMPORTANCE M48 metalloproteases are widely distributed in all domains of life. E. coli possesses four members of this family located in multiple cellular compartments. The functions of these proteases are not well understood. Recent investigations revealed that one family member, BepA, has an important role in the maturation of a central component of the lipopolysaccharide (LPS) biogenesis machinery. Here, we present the structure of BepA and the results of a structure-guided mutagenesis strategy, which reveal the key residues required for activity that inform how all M48 metalloproteases function.

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Sperandeo, P., R. Villa, A. M. Martorana, M. Samalikova, R. Grandori, G. Deho, and A. Polissi. "New Insights into the Lpt Machinery for Lipopolysaccharide Transport to the Cell Surface: LptA-LptC Interaction and LptA Stability as Sensors of a Properly Assembled Transenvelope Complex." Journal of Bacteriology 193, no. 5 (December 17, 2010): 1042–53. http://dx.doi.org/10.1128/jb.01037-10.

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