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CARDOSO, MENDES MOURA ELISABETE CRISTINA. "TARGETING THE LIPOPOLYSACCHARIDE TRANSPORT TO DEVELOP NOVEL ANTIMICROBIAL DRUGS." Doctoral thesis, Università degli Studi di Milano, 2021. http://hdl.handle.net/2434/789419.
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The emergence of multidrug-resistant strains of Gram-negative pathogens that rapidly spread in the clinic is of great concern, since the range of antibiotics still effective against these organisms is limited and will continue to diminish. Therefore, the identification of novel and unexplored drug targets is an urgent need. Gram-negative bacteria possess an outer membrane (OM) with highly selective permeability properties due to its asymmetric structure with LPS in the outer leaflet and phospholipids in the inner leaflet. Dissecting the biogenesis of the OM and gaining insights into the multiprotein machineries that assemble this structure is vital if we want to succeed in developing novel antibiotic compounds that can target these machineries. This thesis focuses on the machinery that transports lipopolysaccharide (LPS) to the cell surface: the LPS transport (Lpt) machinery. In Escherichia coli, the Lpt system is composed of seven essential proteins spanning the cell envelope: the ABC transporter LptB2FGC powers the LPS extraction from the inner membrane (IM) and its transport along the periplasmic bridge, comprising LptA, to the OM LptDE translocon, which assembles LPS on the cell surface.
Due to its vital role in cell physiology, the Lpt system represents a good target for the development of antibiotics with an innovative mechanism of action. Encouragingly, two promising inhibitors of this machinery have been discovered: murepavadin, which is currently in preclinical development, and thanatin. The research project of this thesis focuses on two main topics: elucidating the mechanism behind thanatin’s antibacterial activity, and the characterization of a mutant six-component Lpt machinery that is functional without LptC.
Thanatin is a host-defence antimicrobial peptide recently shown to cause defects in membrane assembly and to bind to the N-terminal β-strand of LptA in vitro (Vetterli et al., 2018). Since this region is involved in both LptA dimerization and interaction with LptC, we implemented the Bacterial Adenylate Cyclase Two-Hybrid (BACTH) system to detect these interactions in the periplasm and probe which is the target of thanatin. With this technique, we found that thanatin targets both interactions and has a stronger inhibitory effect on the LptC-LptA interaction (Moura et al., 2020: https://doi.org/10.3389/fmicb.2020.00909). Further demonstrating a direct effect upon the LPS transport, we observed in thanatin-treated cells the degradation of LptA and the accumulation of LPS decorated with colanic acid (Moura et al., 2020), both of which have been previously reported to be indicative of LPS transport defects (Sperandeo et al., 2008, 2011). We further explored how thanatin affects the integrity of the cell envelope and observed that it induces promoters regulated by envelope-specific stress response systems (unpublished data).
Although all seven Lpt proteins have been shown to be essential, viable mutants lacking LptC but carrying suppressor mutations at the residue R212 in the periplasmic domain of LptF were isolated by our group (Benedet et al., 2016). Interestingly, LptC was recently proposed to have a regulatory role on the LptB2FGC transporter by modulating its ATPase activity (Owens et al., 2019; Li et al., 2019), thus adding to the mystery of how the suppressor mutants can survive without LptC. In the second part of the project, we elucidated how the cell can bypass the presence of LptC and its regulatory role in the machinery by performing a biochemical characterization of the most representative suppressor mutant (manuscript ready for submission). Moreover, by analysing the interaction networks around the residue R212 of LptF, we also formulated a putative mechanism adopted by the Lpt transporter to regulate LPS transfer from LptB2FGC to LptA.
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Sreeramakavacham, Bindu. "FILM GROWTH OF NOVEL FREQUENCY AGILE COMPLEX-OXIDE PIEZOELECTRIC MATERIAL." Master's thesis, University of Central Florida, 2007. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/3104.
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Piezoelectric materials are well known for their applications in surface (SAW) and bulk acoustic wave (BAW) devices such as oscillators, resonators and sensors. Quartz has been the main material used in such applications. Ternary calcium gallium germanate (CGG) structure-type materials, so-called langasites, recently emerged as very promising because of their piezoelectric properties superior to quartz. This thesis discusses the growth of langasite-type La3Ga5.5Ta0.5O14 (LGT) films by liquid phase epitaxy (LPE) technique and their chemical and structural characterization. In addition, the different techniques suitable for the growth of LGT are discussed and compared. To adjust the materials properties for given applications, doping by selected ions can be used. However, the dopants must be homogeneously distributed. In the current study, Al, Ti, Cr and Ca were investigated as dopants. In an earlier study, Al and Ti had been chosen because of their ability to substitute the octahedral site of LGT, normally occupied by Ga (CN=VI) with a segregation coefficient near unity in Czochralski growth. Doping with Ca and Cr has never been reported before, and therefore, the segregation behavior was unknown. In this study, Al, Ti and co-doping with Cr and Ca has been investigated for both X and Y-oriented films. The dopant distribution in the films was quantitatively evaluated by Secondary Ion Mass Spectroscopy (SIMS), using ion-implanted LGT substrates as standards. The drop of dopant concentration, in the SIMS profile, allows for the identification of the film-substrate interface and to accurately measure the thickness of the films. The film thickness is found to be typically of the order 0.5 to 2µm, depending on growth conditions. The solvent was found a reliable choice, as solvent ions were not incorporated in the films above the detection limits of the characterization techniques. A lead oxide solvent system is used as a solvent for the growth of LGT LPE films with different orientations. Extensive structural characterization was performed. The crystallinity of substrates and films grown with different orientations was compared by X-ray diffraction (XRD). The films show a very high structural perfection, with typically FWHM values of 0.035 for the (004) reflection of the XRD rocking curve. The films were also characterized by TEM. The optical transmittance of the films was characterized by Varian optical spectrophotometer, and the value obtained of approximately 80% is comparable with the transmittance value of the Czochralski grown polished substrate.M.S.M.S.E.
Department of Mechanical, Materials and Aerospace Engineering
Engineering and Computer Science
Materials Science & Engr MSMSE
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Bodewits, Karin. "Biosynthesis pathway & transport of endotoxin : promising antibacterial drug targets in the Burkholderia cepacia complex (BCC)." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/5791.
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Burkholderia cepacia complex (Bcc) species are opportunistic pathogens in patients with cystic fibrosis (CF), which are able to cause lethal infections. The Bcc are inherently resistant to most classes of antibiotics, which makes successful treatment problematic. Lipid A (also known as endotoxin), the hydrophobic anchor of lipopolysaccaride (LPS), is the bio-active component of LPS. One of several unique characteristics of the lipid A of the Bcc, is the permanent attachment of 4-amino-4-deoxy-L-arabinose (L-Ara4N) to the lipid A molecule. Also, the genes involved in L-Ara4N biosynthesis are necessary for viability in B. cenocepacia. Here we present research on lipid A biosynthesis, modi cation, and transport in the Bcc and highlight promising antimicrobial targets. The synthetic antibiotic CHIR-090 is an inhibitor of LpxC, an enzyme involved in the lipid A biosynthetic pathway. I investigated the activity of CHIR-090 against the Bcc and found that sensitivity to this antibiotic was both species- and strain-specific. CHIR-090 displayed MICs between 0.1 and 12.5 μg/ml against a panel of B. multivorans, the most prevalent Burkholderia species in CF. The species- and strain-specific sensitivity towards CHIR-090 was further explored and a strong correlation was found between the presence of a unique open reading frame, named LpxC2, in resistant species. To address the problem of multiple drug-resistance of the Bcc, we investigated the activity of the pyridoxal 50-phosphate (PLP)-dependent enzyme inhibitor cycloserine (CS) against the Bcc. CS is used as a second line of defense against M. tuberculosis. The activity of the D-enantiomer of CS (DCS) against the Bcc was tested and displayed MICs between 2 and 128 μg/ml and acted bactericidal towards the Bcc. Additionally, DCS inhibition of recombinant ArnB from B. cenocepacia J2315, a PLP-dependent enzyme necessary for viability in the Bcc, was studied. ArnB was inhibited reversibly by DCS. ArnB was further explored as a promising drug-target in the Bcc, but only CS has been identified as an inhibitor so far. In this thesis it was attempted to find the reason why is L-Ara4N modification of lipid A necessary for viability in B. cenocepacia. Therefore, two proteins were characterised, which are involved in lipid A transport: LptA, the periplasmic lipid A binding protein, and LptB, the cytoplasmic ATP-ase. LptA was found to be able to bind both modified and unmodified lipid A in vitro and therefore is not L-Ara4N specific. Furthermore, LptA could bind deep-rough-, rough-, and smooth- LPS, similar to that described for Escherichia coli LptA. The kinetic parameters of LptB were determined in vitro (kcat = 5.71 min-1 and KM = 0.88 mM), and were comparable to E. coli LptB. The ATP-ase activity of LptB was not influenced by the presence of any forms of LPS (modified or non-modified). Therefore, we concluded that both B. cenocepacia J2315 LptA and LptB are not L-Ara4N specific.
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Shen, Xueying. "Complex lot Sizing problem with parallel machines and setup carryover." Thesis, Paris Sciences et Lettres (ComUE), 2017. http://www.theses.fr/2017PSLED057/document.
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Dans cette thèse, nous étudions deux problèmes de planification de production motivés par des applications du monde réel. Tout d'abord, un problème de planification de production pour un projet de fabrication de vêtements est étudié et un outil d'optimisation est développé pour le résoudre. Deuxièmement, une version restreinte du problème de dimensionnement du lot de capacité avec des configurations dépendantes de la séquence est explorée. Diverses formulations mathématiques sont développées et une analyse de complexité est effectuée pour donner une première analyse du problèmeIn this thesis, we study two production planning problems motivated by challenging real-world applications. First, a production planning problem for an apparel manufacturing project is studied and an optimization tool is developed to tackle it. Second, a restricted version of the capacitated lot sizing problem with sequence dependent setups is explored. Various mathematical formulations are developed and complexity analysis is performed to offer a first glance to the problem
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Kelm, Travis R. "LOT complexes and the Whitehead conjecture /." view abstract or download file of text, 2002. http://wwwlib.umi.com/cr/uoregon/fullcit?p3055693.
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Thesis (Ph. D.)--University of Oregon, 2002.Typescript. Includes vita and abstract. Includes bibliographical references (leaves 71-73). Also available for download via the World Wide Web; free to University of Oregon users.
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Charles, Mehdi. "Modeling and solving complex multi-item lot-sizing problems with inventory constraints." Thesis, Lyon, 2021. http://www.theses.fr/2021LYSEM039.
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Nous nous sommes intéressés au problème de lot-sizing multi-produits avec capacité, temps de lancement et ventes perdues. Nous avons étendu ce problème afin de prendre en compte des aspects industriels importants, en particulier des contraintes sur les stocks. Nous avons d'abord étudié les effets de fin d’horizon des solutions aux problèmes de lot-sizing, qui peuvent entraîner des coûts importants même pour des horizons temporels très longs. Pour compenser ces effets, nous avons proposé de rajouter une contrainte de stock final minimal ainsi que des contraintes de stock maximal par produit. Ces valeurs ont été déduites d'une analyse d'un problème de lot-sizing cyclique avec temps de lancement et capacité dont la relaxation linéaire peut être résolue de manière analytique. Par la suite, nous nous sommes intéressés à la modélisation de l'évolution des stocks intra-périodes. Cet aspect est particulièrement important lorsque les capacités de stockage sont limitées. Nous avons proposé des nouvelles contraintes qui différent en fonction des hypothèses sur la production et la demande. L'objectif est de limiter les excès et les déficits de stock lors de l'ordonnancement détaillé du plan de production à chaque période. Nos résultats numériques ont montré que ces nouvelles contraintes permettent de construire des plans de production respectant davantage les contraintes sur les stocks. Des méthodes de résolution génériques et plus particulièrement des méthodes de décomposition (relaxation Lagrangienne, relax-and-fix) ont été développées. Une approche originale de parallélisation a été proposée, dont l’objectif est de réduire la taille des sous-problèmes à résoudre et d'utiliser les outils disponibles à DecisionBrain. L'objectif final de cette thèse a été l'implémentation des heuristiques proposées dans l'outil d'optimisation développé par DecisionBrain ainsi que des tests de performance sur des instances industriellesIn this thesis, we considered the capacitated multi-item lot-sizing problem with setup times and lost sales. We extended this problem to take into account important industrial aspects, especially with regards to inventory management. We first studied the end-of-horizon effects on optimal solutions of lot-sizing problems that, even on a rolling horizon, can lead to important additional costs. To reduce these effects, we have added a global minimum ending inventory constraint as well as a maximum ending inventory constraint for each item. These values were deduced from the analysis of a cyclical capacitated lot-sizing problem with setup times, whose linear relaxation can be analytically solved. Then, we modeled the inventory evolution within each period. This point is especially relevant when the storage capacity is limited. We added new inventory constraints to better respect inventory bounds when scheduling productions within each period. The constraints differ based on hypotheses on the shapes of evolution of production and demand. Numerical experiments showed that these new constraints enable to schedule production plans with a better inventory management. Decomposition approaches (Lagrangian relaxation, relax-and-fix) were developed in order to propose generic approaches to solve capacitated lot-sizing problems with setup times. An original use of parallelization was proposed in order to reduce the size of the subproblems to solve and to use Decisionbrain's tools.Finally, the parallelized relax-and-fix was implemented into DecisionBrain's optimization tool and tests were performed on industrial instances
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Yang, Yiying. "Mécanismes de biogenèse et de maintien de la membrane externe des bactéries à Gram négatif." Thesis, Toulouse 3, 2021. http://www.theses.fr/2021TOU30279.
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Les bactéries à Gram négatif comprennent un certain nombre d'agents pathogènes animaux redoutables qui sont particulièrement résistants aux thérapies antibiotiques grâce à la fonction protectrice de leur enveloppe bactérienne. L'enveloppe des bactéries à Gram négatif est composée d'une membrane interne (MI) et d'une membrane externe (ME), séparées par un espace, le périplasme, contenant le peptidoglycane (PG). Le feuillet externe de la bicouche de la ME contient des lipopolysaccharides (LPS) qui forment une barrière imperméable à certaines molécules toxiques, tels que les détergents ou des petites molécules hydrophobes. Les nutriments sont transportés par les protéines (OMP) de la ME. D'autres OMP remplissent des fonctions de biogenèse de l'enveloppe, notamment l'assemblage des OMP et des LPS. Les OMP sont assemblées dans la ME par le complexe d'assemblage en tonneau bêta (BAM), un hétéro-pentamère contenant l'OMP essentielle BamA et quatre lipoprotéines BamBCDE. L'assemblage du LPS nécessite une autre OMP essentielle, LptD, qui s'associe de manière stable avec la lipoprotéine LptE. Un assemblage défectueux des OMP provoque un stress de l'enveloppe et rend les bactéries Gram-négatives sensibles aux antibiotiques et aux détergents. Le complexe BAM représente donc une cible prometteuse pour le développement de nouveaux antibiotiques. La fonction moléculaire du complexe BAM dans la biogénèse des ME est mal comprise. En utilisant une stratégie de spectrométrie de masse quantitative, le laboratoire d'accueil a récemment identifié deux nouveaux interacteurs putatifs du complexe BAM, les lipoprotéines DolP (anciennement YraP) et YifL, toutes deux de fonctions inconnues. L'objectif de ce travail de thèse était de caractériser les rôles respectifs de DolP et YifL au sein du complexe BAM. DolP est une lipoprotéine de la ME de 20 kDa. L’accumulation d’OMP mal repliées dans le périplasme induit un stress de l’enveloppe qui, à son tour, stimule la production à la fois de BamA et de DolP. Cependant, le rôle de cette dernière dans la biogenèse des OMP n’est pas connu. Dans cette étude, en utilisant un crible génétique, nous avons montré que DolP est critique pour la croissance des cellules qui subissent un stress d'enveloppe. Nous avons montré que l’accumulation de BamA dans la ME est toxique pour les cellules mais que DolP est capable de contrecarrer cette toxicité en favorisant le bon repliement de BamA (Ranavaco-first; Yangco-first; Orenday-Tapiaco-first, et al., 2021). En parallèle, la caractérisation de l'interaction BAM-YifL a permis de montrer que YifL interagit avec BamA et BamD. De manière intéressante, nous avons découvert que YifL interagit également avec la machinerie de sécrétion des LPS, LptDE, qui est assemblée par le complexe BAM dans la ME. Ce processus nécessite également la machinerie de repliement oxydatif DsbA pour oxyder les 4 résidus de cystéine dans LptD de façon séquentielle. Une analyse génétique a montré que la délétion de yifL exacerbe la sensibilité des cellules bamB ou dsbA au SDS, un détergent normalement exclu par la couche de LPS. Nous avons constaté qu'en l'absence de YifL, LptD se fixe transitoirement au complexe BAM sous une forme intermédiaire partiellement oxydée. L'oxydation efficace de LptD pour former une protéine mature et fonctionnelle nécessite YifL. Nos résultats révèlent pour la première fois que YifL est critique pour la biogenèse efficace du complexe LptDEGram-negative bacteria include a number of dreadful animal pathogens that are particularly resistant to antibiotic therapies thanks to the sheltering function of their bacterial envelope. The envelope is composed of an inner and an outer membrane (IM and OM), and the separating periplasm containing the peptidoglycan (PG). The outer leaflet of the OM bilayer largely consists of lipopolysaccharide (LPS) that forms a permeability barrier against toxic molecules, including detergents and small hydrophobic molecules. Nutrients are transported via OM-spanning proteins (OMPs). Other OMPs perform envelope biogenesis functions, including the assembly of OMPs and LPS. OMPs are assembled into the OM by the beta-barrel assembly machinery (BAM), a heteropentamer containing the essential OMP BamA and four lipoproteins BamBCDE. The assembly of LPS requires another essential OMP, LptD, which stably associates with the lipoprotein LptE. Defective assembly of OMPs causes envelope stress and renders Gram-negative bacteria sensitive to antibiotics and detergents. Hence, the BAM complex represents a promising target for the development of new therapies. The mechanistic details of how the BAM complex functions ensuring efficient OM biogenesis are only marginally understood. By using a quantitative mass-spectrometry strategy the hosting lab has recently identified two novel putative interactors of the BAM complex of Escherichia coli, the lipoproteins DolP (formerly YraP) and YifL, both of unknown functions. The aim of this PhD thesis work was to characterize the roles of DolP and YifL at the BAM complex. DolP is a ~20 kDa OM lipoprotein that localizes in the periphery of E. coli cells and accumulates at the mid-cell specifically during a late step of cell division. DolP is upregulated during envelope stress caused by the accumulation of unfolded OMPs in the periplasm. Whether DolP plays any role in OMP biogenesis, however, was unknown. In this study, by using a genetic screen, we have shown that DolP is critical for the fitness of cells that undergo envelope stress. We have demonstrated that an increment of BamA in the OM, which is also upregulated during envelope stress, is potentially toxic for the cells. We provide evidence that DolP promotes proper folding and function of BamA thereby counteracting its toxicity. The mid-cell recruitment of DolP had been linked to regulation of septal peptidoglycan hydrolysis by an unknown mechanism. Our study reveals that during envelope stress DolP loses its association with the mid-cell, thus revealing a mechanistic link between impaired OMP biogenesis and a late step of cell division (Ranavaco-first; Yangco-first; Orenday-Tapiaco-first, et al., 2021). Next the BAM-YifL interaction was characterized. We showed that the ~7 kDa YifL interacts with BamA and BamD. Interestingly, we have found that YifL also interacts with the LPS secretory machinery LptDE, which is assembled by the BAM complex into the OM
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Nebenfuhr, Mario T. "The LPS-Receptor Complex on Human Monocytes Investigated using Single Particle Fluorescence Imaging and Proteomics." Thesis, University of Essex, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.520101.
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Attallah, Aly [Verfasser]. "Distributed Control for Complex Mission Scenarios With Non-Holonomic Agents - An LPV Approach / Aly Attallah." München : Verlag Dr. Hut, 2021. http://d-nb.info/1238423078/34.
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Carvalho, Jozélio Freire de. ""Antilipoproteína lipase (LPL): um novo componente no complexo processo aterosclerótico do lúpus eritematoso sistêmico?"." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/5/5145/tde-27092005-130705/.
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Dislipidemia é implicada no processo aterosclerótico do LES. A descrição de aLPL no LES associado a hipertrigliceridemia levou-nos a analisar esse anticorpo no contexto da inflamação envolvida na aterogênese. aLPL foi encontrado em 38% dos pacientes com LES com altos níveis de triglicérides. Correlação positiva significante foi observada entre aLPL e PCR, VHS, SLEDAI, anti-DNA, anti-cardiolipina e CH100 baixo. Análise de regressão múltipla confirmou a forte associação entre aLPL e PCR. Esses dados dão suporte à associação entre inflamação, resposta imune e dislipidemia, introduzindo o aLPL como um novo componente nos complexos eventos da aterogênese do LESDyslipidemia is implicated in the atherosclerosis process of SLE. The description of aLPL in SLE associated with hypertrigliceridemia prompted us to analyze this antibody in the context of the inflammation involved in the atherogenesis. aLPL was found in 38 por cento of SLE patients with high levels of triglycerides. Significant positive correlation was observed between aLPL and CRP, ESR, SLEDAI, anti-DNA, anti-cardiolipin and low CH100. Multiple regression analysis confirmed the strong association between aLPL and CRP. These data support the link between inflammation, immune response and dyslipidemia, introducing anti-LPL as new player in the complex events of atherogenesis in SLE
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Santos, Maria Verônica dos. "Imunização nasal com antígenos de membrana externa de Neisseria meningitidis B selecionados para a maior expressão do imunotipo de LPS 3, 7, 9 com anticorpos monoclonais e Bordetella pertussis como adjuvante em camundongos neonatos." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-13012009-155309/.
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O habitat natural da Neisseria meningitis é a nasofaringe humana e a transmissão da bactéria é por contato direto ou por inalação de partículas durante a fase de transmissão N. meningitis é uma bactéria Gram-negativa responsável por uma significante mortalidade em todo o mundo. Embora existam vacinas polissacárides contras os sorogrupos A, C, W135 e Y , não há uma vacina adequada para crianças menores de 4 anos para o sorogrupo B. Estudos estão sendo direcionadas para pesquisa de antígenos vacinais que são derivados da proteínas de membrana externa(NOMV). Entretanto vacinas baseadas em NOMV são consideradas pouco imunogênicas , fazendo com que o uso de adjuvantes seja necessário. Este estudo investiga a imunogenicidade da NOMV de N. meningitidis administrada pela via intranasal/intramuscular em camundongos neonatos BALC/c, usando proteína de membrana externa (NOMC) obtido de uma cepa epidêmica de N. meningitidis B:4:P1:15. As cepas usadas para imunização dos camundongos foram selecionadas por colony-blot, usando anticorpo monoclonal anti L3,7,9 para maior expressão do LPS contra o imunotipo L3,7,9 presente na cepa (B:4:P1:15 3,7,9). Como adjuvantes de mucosa foram utilizados Bordetella pertussis (células íntegras) ou sobrenadante de cultura com 48 horas ou hidróxido de alumínio [Al(OH)3]. O soro dos camundongos imunizados foram analisados pelo método de ELISA à fim de se comparar os diferentes adjuvantes utilisados. O índice de avidez também foi determinado. IgG e IgM foram detectados nos soros dos camundongos após imunização, com índices de intermediária e alta avidez. Todos os adjuvantes foram capazes de aumentar a resposta imune contra NOMV de N.meningitidis. A via intranasal foi adequada para sensibilizar as células do sistema imune que foram rapidamente estimuladas pela via intramuscular usando os adjuvantes utilizados na presente investigação. Dados sugerem que o estudo da NOMV é importante na indução da imunidade de mucosa para N. meningitidis B, e que a qualidade e magnitude da resposta imune gerada pelas vacinas de mucosa são influenciadas tanto pelo adjuvante como pelo antígeno. Concluímos que NOMV juntamente com adjuvantes de mucosa tem considerável potencial no desenvolvimento de vacinas contra o meningococo do sorogrupo B.The natural habitat of Neisseria meningitidis is the human nasopharynx, and the bacterium is transmitted by direct mouth-to-mouth contact or by the inhalation of released mucous particles during close contact. N meningitidis is a Gram-negative bacterium responsible for significant mortality worldwide. While effective polysaccharide-based vaccines exist against serogroups A, C, W135, and Y, no similar vaccine is suitable for children under 4 years against disease caused by serogroup B strains. Current studies are searching for vaccinal antigens that are derived from the native outer membrane (NOMV). However, vaccines based on NOMV are considered weak, making the use of adjuvants necessary. This study investigated the immunogenicity of NOMV of N. meningitidis administered intranasal/intramuscular in neonate BALB/c mice, using the native outer membrane complex (NOMC) obtained from an epidemic strain of N. meningitidis B:4:P1.15. The strains used for immunization of mice were selected by colony-blot, using anti L3,7,9 monoclonal antibodies, for the highest expression of LPS among the immunotypes (B:4:P1:15 L9á). As mucosal adjuvants, we used Bordetella pertussis (whole cells) or the supernatant of 48 h culture of this bacterium, followed by an intramuscular dose of the same protein adsorbed onto , B. pertussis (whole cells) or 48-h B. pertussis culture supernatant or aluminum hydroxide [Al(OH)3]. Sera of immunized mice were evaluated by ELISA in order to compare the different adjuvants used. We also determined their avidity index. IgG and IgM were detected in the serum of mice after immunization, with avidity indices that ranged from intermediate to high. All adjuvants were capable of increasing the immune response against NOMV of N. meningitidis in the homologous prime/boost schedule used. The intranasal route was suitable for sensitizing the cells of the immune system which were quickly stimulated by the intramuscular route using the adjuvants analysed in the present invertigation. Data suggest that the NOMV studied is important in the induction of mucosal immunity to N. meningitidis B, and that the quality and magnitude of the immune responses generated by mucosal vaccines are influenced by the adjuvant as well as the antigen. In conclusion, nasal delivery of NoMV with mucosal adjuvants has considerable potential in the development of a mucosal vaccine against serogroup B meningococci.
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Attallah, Aly [Verfasser]. "Distributed control for complex mission scenarios with non-holonomic agents – an LPV approach / Aly Saeed Aly Aly Attallah." Hamburg : Universitätsbibliothek der Technischen Universität Hamburg-Harburg, 2021. http://nbn-resolving.de/urn:nbn:de:gbv:830-882.0135993.
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Singh, Shalini. "Amphiphilic Peptide Interactions with Complex Biological Membranes : Effect of peptide properties on antimicrobial and anti-inflammatory effects." Doctoral thesis, Uppsala universitet, Institutionen för farmaci, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-282781.
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With increasing problem of resistance development in bacteria against conventional antibiotics, as well as problems associated with diseases either triggered or enhanced by infection, there is an urgent need to identify new types of effective therapeutics for the treatment of infectious diseases and its consequences. Antimicrobial and anti-inflammatory peptides have attracted considerable interest as potential new antibiotics in this context. While antimicrobial function of such peptides is being increasingly understood demonstrated to be due to bacterial membrane disruption, the mechanisms of their anti-inflammatory function are poorly understood. Since bacterial membrane component lipopolysaccharide triggers inflammation, this thesis aims at clarifying importance of lipopolysaccharide (LPS)-peptide interactions while investigating possible modes of action of peptides exhibiting anti-inflammatory effect. Furthermore, effect of poly(ethylene)glycol (PEG)-conjugation was investigated to increase performance of such peptides. Results presented in this thesis demonstrate that peptide-induced LPS- and lipid A binding/scavenging is necessary but not sufficient criterium for anti-inflammatory effects of peptides. Furthermore, preferential binding to LPS over lipid membrane, as well as higher binding affinity to the lipid A moiety within LPS, are seen for these peptides. In addition, results demonstrate that apart from direct LPS scavenging, membrane-localized peptide-induced LPS scavenging seem to contribute partially to anti-inflammatory effect. Furthermore, fragmentation and densification of LPS aggregates, in turn dependent on the peptide secondary structure on LPS binding, as well as aromatic packing interactions, correlate to the anti-inflammatory effect, thus promoting peptide-induced packing transition in LPS aggregates as key for anti-inflammatory functionality. Thus, peptide-induced LPS aggregate disruption together with reduction of the negative charge of LPS suggests the importance of phagocytosis as an alternative to the inflammatory pathway, which needs to be further investigated. Furthermore, PEG conjugation of peptide results in strongly reduced toxicity at a cost of reduced antimicrobial activity but markedly retained anti-inflammatory effect. Taken together, the results obtained in this work have demonstrated several key issues which need to be taken into consideration in the development of effective and selective anti-inflammatory peptide therapeutics for the treatment of severe Gram-negative bacterial infections.
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Briand, Bernard. "Caractérisation des métamagmatites en domaine profond et reconnaissance des sites géotectoniques : exemple du complexe leptyno-amphibolique de la vallée du Lot (Massif Central français)." Lyon 1, 1986. http://www.theses.fr/1986LYO19039.
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Le "groupe leptyno-amphibolique" de la vallee du lot est une association magmatique bimodale affectee par une evolution metamorphique de haut degre. Il est constitue des metagabbros metadolerites, pyrigarnites, eclogites et leptynites, encaisses dans un ensemble de gneiss d'origine sedimentaire et volcano-sedimentaire. La composition lithologique et les caracteres de ses constituants excluent, pour ce complexe, toute analogie avec les associations ophiolitiques; ils sont en acord avec un cadre de mise en place ensialique, dans un environnement de type marginal
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Petrauskas, Karolis. "Computational Modelling of Biosensors of Complex Geometry." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2011. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20110701_105911-89480.
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Biosensors are analytical devices mainly used to detect analytes and measure their concentrations. Mathematical modeling is widely used for optimizing and analyzing an operation of biosensors for reducing price of development of new biosensors. The object of this research is mathematical and computer models, describing an operation of biosensors, made of several parts with different properties. The dissertation covers models, formulated in one and two-dimensional spaces by partial differential equations with non-linear members, and solved numerically, using the method of finite differences. The numerical models are implemented by a computer program. An original mathematical model for a biosensor with a carbon nanotube electrode is presented in the dissertation. The conditions at which the one-dimensional mathematical model can be used instead of two-dimensional one for accurate prediction of the biosensor response are investigated. Elements, used to build models of biosensors with a complex structure, were systemized. The biosensor description language is proposed and the computer software, simulating an operation of biosensors in the one-dimensional space and a rectangular domain of the two-dimensional space, is developed. An adequateness of the model for the biosensor with the carbon nanotube electrode and the impact of structural and geometrical properties on a response of the biosensor were investigated, performing computer experiments using the developed software.Biojutikliai yra įrenginiai, skirti medžiagoms aptikti bei jų koncentracijoms matuoti. Siekiant sumažinti biojutiklių gamybos kaštus yra pasitelkiamas matematinis biojutikliuose vykstančių procesų modeliavimas. Disertacijoje nagrinėjami matematiniai ir kompiuteriniai biojutiklių modeliai, aprašantys biojutiklių, sudarytų iš kelių, skirtingas savybes turinčių dalių, veikimą. Nagrinėjami modeliai yra formuluojami vienmatėje bei dvimatėje erdvėse, aprašomi diferencialinėmis lygtimis dalinėmis išvestinėmis su netiesiniais nariais ir yra sprendžiami skaitiškai, naudojant baigtinių skirtumų metodą. Skaitiniai modeliai yra įgyvendinami kompiuterine programa. Disertacijoje pateikiamas originalus matematinis modelis biojutikliui su anglies nanovamzdelių elektrodu, nustatyti kriterijai, apibrėžiantys, kada biojutiklį su perforuota membrana galima modeliuoti vienmačiu modeliu. Darbe susisteminti elementai, naudojami biojutiklių modelių formulavimui, pagrindinį dėmesį skiriant biojutiklio struktūrinėms savybėms modeliuoti. Apibrėžta biojutiklių modelių aprašo kalba ir sukurta programinė įranga, leidžianti modeliuoti biojutiklių veikimą vienmačiais modeliais arba modeliais, formuluojamais stačiakampėje dvimatės erdvės srityje. Taikant sukurtą biojutiklių modeliavimo programinę įrangą, ištirtas biojutiklio su anglies nanovamzdelių elektrodu modelio adekvatumas ir struktūrinių bei geometrinių savybių įtaka biojutiklio elgsenai.
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Chantot, Sandrine. "Aspects cytogenetiques des leucemies prolymphocytaires - b (lpl-b) : etude sequentielle en fish - multiplex (m - fish) des anomalies chromosomiques complexes dans un cas de lpl-b evolutive." Paris 5, 1999. http://www.theses.fr/1999PA05N086.
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Gaidamauskaitė, Evelina. "Computational Modeling of Complex Reactions Kinetics in Biosensors." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2011. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20111122_102523-68545.
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Biosensors are analytical devices made up of a combination of a biological entity, usually an enzyme, that recognizes a specific analyte (substrate) and the transducer that translates the biorecognition event into a signal. In order to create new types of biosensors the corresponding experimental studies are necessary. Computational experiments could very well replace very expensive physical ones. However, the multi-step character of a chemical reaction scheme must be considered and modeled accordingly. In this thesis such reaction schemes were studied in great details. Original mathematical models were developed for optical peroxidase-based and amperometric laccase-based biosensors. The deterministic nature of model construction allows the automated models to be built. Based on this assumption flexible model for computational modeling of different practical multistep biosensors was developed. In order to optimize the numerical solution of the reaction-diffusion type equations common finite difference schemes were compared. The comparison shows that the fastest schemes to achieve the required relative error are implicit and Hopscotch schemes. For the problems where accuracy is not a significant factor but the speed is, the simplest explicit scheme should be used. Applying the new flexible model a computational modeling of the multi-step biosensors were produced. The modeling of laccase biosensor explained and confirmed the synergistic effect. The computational modeling of the... [to full text]Biojutikliai yra analitiniai įtaisai sudaryti iš biologiškai aktyvios bei selektyviai atpažįstančios substratą medžiagos, dažniausiai fermento, ir keitiklio formuojančio makroskopinį fizinį signalą. Naujų įtaisų kūrimui būtini lygiagretūs eksperimentiniai tyrimai. Skaitiniai eksperimentai gali patikimai pakeisti fizinius. Modeliuojant tokius biojutiklius, būtina atsižvelgti į juose vykstančių procesų daugiapakopį pobūdį. Šiame darbe nuodugniai ištirtos tokių reakcijų schemų savybės. Sudaryti originalūs matematiniai modeliai optiniam peroksidaziniam bei amperometriniam lakaziniam daugiapakopiams biojutikliams. Deterministinė modelių sudarymo proceso prigimtis leidžia jį automatizuoti. Remiantis šiuo principu sukurtas bendras įrankis kompiuteriniam daugiapakopių biojutiklių modeliavimui. Siekiant optimizuoti skaitinį sprendimą palygintos dažniausiai naudojamos baigtinių skirtumų skaitinio sprendimo schemos sprendžiant reakcijos - difuzijos lygtis. Pastarasis palyginimas parodė, kad greičiausiai reikiamas sprendinio tikslumas pasiekiamas taikant neišreikštinę bei Hopscotch schemas. Uždaviniams, kuriems sparta svarbesnė už tikslumą, turėtų būti taikoma išreikštinė schema. Taikant naują įrankį atliktas kompiuterinis daugiapakopių biojutiklių modeliavimas. Kompiuterinis lakazinio biojutiklio modeliavimas teoriškai paaiškino eksperimentiškai stebėtą sinergetinę mediatoriaus įtaką biojutiklio atsakui. Peroksidazinio biojutiklio kompiuterinio modeliavimo rezultatai parodė, kad plataus... [toliau žr. visą tekstą]
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Kazlauskienė, Eglė. "Sorption of metal complex dyes onto ion exchanger resins." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2012. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2012~D_20120131_092413-89661.
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Metal complex dyes are a problematic group of substances, present in the negatively charged anion form in mixed industrial wastewater that should be removed. Therefore, a complete removal of these hazardous dyes from wastewaters is necessary to prevent them from release into the environment. Adsorption on activated carbon of dyes has been investigated widely. However some disadvantages using activated carbon in practice were observed, e.g. high regeneration costs and production of fines due to the brittle nature. Since their adsorption capacities, mechanical strength, and other properties need further improvement for wider application, the polymeric sorbents are still under development as a potential alternative to activated carbons. The main aim of the present work was to investigate absorption regularity metal complex Lanasyl Navy M-DNL and Acid Blue 249 (copper (II) phthalocyanine) dyes on synthetic ion exchangers under static conditions and evaluate facility of ion exchangers to removal dyes from wastewaters by dynamic conditions. Screening with chromium-complex dye (acid brown NKM, Lanasyn Navy M-DNL) solutions wide range sorbents, according to the physical chemical characteristics of sorbents and established dye removal parameters (sorption capacity, distribution coefficient and sorbent regeneration) has shown that the polyacrylic, gel type, weakly basic anion exchanger Purolite A 847 (A 845); polystyrene, macroporous, strongly basic anion exchanger Purolite A 500 PS... [to full text]Pagrindinis aplinkosauginės politikos bruožas yra taršos metalo dažikliais mažinimas gamybos metu ir efektyvių šalinimo iš nuotekų metodų paieška. Regeneruojami, mechaniškai atsparūs ir nekenksmingi gamtai polimeriniai sorbentai naudojami dažikliams valyti yra pranašesni už plačiai paplitusias aktyvintąsias anglis. Ištyrus polimerinių sorbentų sorbcines ir regeneracines savybes, nustačius palankias darbines sąlygas, sorbentai gali būti pritaikomi nuotekų valymui, o išvalytas vanduo grąžinamas atgal į gamybinius procesus. Tokiu būdu, vandens ištekliai gali būti apsaugoti nuo taršos sunkiaisiais metalais ir organiniais junginiais, sumažinti gamybos kaštai, nes regeneruoti sorbentai naudojami pakartotinai. Šiame darbe tiriama metalo kompleksinių dažiklių Navy M-DNL (1:2 chromo monoazo kompleksas) (Navy) ir Acid Blue 249 (Vario (II) ftalocianino tetrasulforūgšties tetranatrio druska) (CuPc) sorbcijos sintetiniais jonitais dėsningumus statinėmis sąlygomis ir įvertinti jonitų galimybes valyti nuotekas nuo dažiklių dinaminėmis sąlygomis. Geriausiai iš vandeninių tirpalų šalinantys dažiklius sorbentai atrinkti pagal geriausią sorbcinė gebą ir regeneraciją, dažiklio pasiskirstymo tirpale koeficientą yra: silpnai baziniai anijonitai Purolite A 847 (A 845), stipriai bazinis anijonitas Purolite A 500PS, nejoninis makrotinklinis sorbentas Macronet MN 200. Statinėmis sąlygomis nustatyta dažiklių (Navy ir CuPc) sorbcijai palankiausia terpė (pH 2) ir temperatūra atitinkamai dažikliui Navy... [toliau žr. visą tekstą]
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Amini, Nahid. "Novel Solid Phase Extraction and Mass Spectrometry Approaches to Multicomponent Analyses in Complex Matrices." Doctoral thesis, Stockholms universitet, Institutionen för analytisk kemi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-38625.
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Analysis of compounds present in complex matrices is always a challenge, which can be partly overcome by applying various sample preparation techniques prior to detection. Ideally, the extraction techniques should be as selective as possible, to minimize the concentration of interfering substances. In addition, results can be improved by efficient chromatographic separation of the sample components. The elimination of interfering substances is especially important when utilizing mass spectrometry (MS) as a detection technique since they influence the ionization yields. It is also important to optimize ionization methods in order to minimize detection limits. In the work this thesis is based upon, selective solid phase extraction (SPE) materials, a restricted access material (RAM) and graphitized carbon black (GCB) were employed for clean up and/or pre-concentration of analytes in plasma, urine and agricultural drainage water prior to liquid chromatography/mass spectrometry (LC/MS). Two SPE formats, in which GCB was incorporated in µ-traps and disks, were developed for cleaning up small and large volume samples, respectively. In addition, techniques based on use of sub-2 µm C18 particles at elevated temperatures and a linear ion trap (LIT) mass spectrometer were developed to improve the efficiency of LC separation and sensitivity of detection of 6-formylindolo[3,2-b]carbazole (FICZ) metabolites in human urine. It was also found that GCB can serve not only as a SPE sorbent, but also as a valuable surface for surface-assisted laser desorption ionization (SALDI) of small molecules. The dual functionality of GCB was utilized in a combined screening-identification/quantification procedure for fast elimination of negative samples. This may be particularly useful when processing large numbers of samples. SALDI analyses of small molecules was further investigated and improved by employing two kinds of new surfaces: oxidized GCB nanoparticles and silicon nitride.
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Kerfai, Syrine. "Etude d'un procédé chromatographique d'échange d'ions pour la séparation de la ribulose 1,5-diphosphate carboxylase (Rubisco) dans le cadre de la valorisation d'un sous produit agricole." Thesis, Toulouse, INSA, 2011. http://www.theses.fr/2011ISAT0014/document.
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Les milieux biologiques bruts, provenant des opérations de transformation de biomasse sont souvent caractérisés à la fois par leur caractère polluant et par un potentiel de valorisation important. Le développement de procédés adaptés au traitement de tels milieux complexes présente ainsi beaucoup d’intérêt. Les jus verts générés par la déshydratation de la luzerne (Medicago Sativa) sont caractérisés par une forte teneur en protéines. Outre leur valeur nutritionnelle importante, ces protéines ont des applications potentielles dans plusieurs domaines, notamment environnemental de part leur teneur élevée en Ribulose 1,5 Bisphosphate Carboxylase Oxydase (Rubisco), enzyme responsable de la fixation du CO2 chez les plantes. Dans ce travail la séparation sélective de la Rubisco à partir du jus de luzerne industriel centrifugé par chromatographie d’échange d’ions a été étudiée. Dans un premier temps une méthode d’analyse qualitative et quantitative a été mise au point pour la détection et la quantification de la Rubisco en solution et ainsi le suivi du procédé de séparation. Dans un deuxième temps, le procédé de séparation a été étudié en colonne, en lit fixe et en lit expansé, en utilisant le support échangeur d’anions Q Hyper Z et l’effet de la dilution du milieu sur la capacité dynamique du procédé a été analysé dans les deux cas. Les résultats obtenus ont montré que les deux modes de contact permettent d’avoir des capacités dynamiques de rétention du même ordre de grandeur que celles de la littérature. Après élution, la Rubisco a été concentrée jusqu’à 21 fois et les fractions produites étaient caractérisées par un grand degré de pureté. Par ailleurs, des études d’équilibre et cinétique d’échange ont été initiées dans ce travail et ont démontré que malgré la taille importante de la protéine d’intérêt (560 kDa) les limitations stériques à son transfert ne sont pas plus importantes que dans le cas de protéines plus simples et plus petites et que le support Q Hyper Z présente effectivement une grande affinité pour la protéine. Enfin une première approche théorique a été conduite pour la compréhension des interactions entre la protéine et l’échangeur dans ce milieu complexe. Elle a permis de confirmer l’importance de la prise en compte de la présence d’autres biomolécules dans le milieu sur la rétention de la Rubisco, peut être même plus que celle des selsBiological raw material derived from bio-refinery processes, is often considered a source of pollution but it seems also to be a promising alternative to potential material recovery. The development of suitable processes for handling such complex biological material has so many concerns. Green juice produced from mechanical dehydration of Alfalfa (Medicago sativa) is an excellent source of protein with high nutritional quality. Ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco) is the most abundant protein in the green juice, with potential applications in many fields, such as human nutrition, pharmaceuticals, environmental… The aim of this study is to isolate and recover Rubisco produced from an industrial alfalfa green juice, by ion exchange chromatography process. First of all, a qualitative and quantitative analytical method was developed to provide reliable information about Rubisco content monitoring in the separation process. In a second step, the separation process was performed in fixed and expanded bed, using the anion exchanger Q Hyper Z. In both cases, the effect of the dilution of the green juice on the dynamic capacity of the columns was studied. The results showed that the dynamic capacity retention was similar in both columns to those reported in literature. After elution step, Rubisco was concentrated 21 times and produced with high level of purity. Furthermore, kinetic of ion exchange study was initiated. Despite the large size of the protein (560 kDa), steric limitations to mass transfer were not very significant when compared to those of conventional small proteins. The support Q Hyper Z showed an excellent affinity for the protein recovery. Finally, a first theoretical investigation has been conducted for understanding the retention mechanism between the protein and the separation column. This study shows the importance of taking into account the presence of other bio-molecules in order to perform the retention of Rubisco, perhaps even more than that of salts
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Žuramskaitė, Laura. "Dieveniškių ir Lazūnų šnektų prijungiamieji sakiniai." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2005. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2005~D_20050621_114009-17942.
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The work is distinguished by its novelty because the syntax of dialects of the Lithuanian language has been hardly explored so far. The goal of this work was to make a thorough description of complex sentences of the East Upper Lithuanians, Vilnius people Dieveniškės and Lazūnai subdialects. While achieving the purpose, the following tasks had to be solved: to explore the typology of complex sentences; to describe the means of subordination; to make an analysis of the structure peculiarity of complex sentences.
The resources used in this work are a book written D.Mikulėnienė and K.Morkūnas “Texts of Dieveniškės subdialects”, published in 1997, “Litauische mundartliche Texte aus der Wilnaer Gegend mit grammatischen Anmerkungen” by Peter Aruma, published in Dorpat in 1930. The latter contains texts of Dieveniškės and Lazūnai subdialects. The third resource that was used is “The vocabulary of Lazūnai dialect” by J.Petrauskas and A.Vidugiris, published in 1985. The material was collected from all the available resources and it comprises 2276 complex sentences.
Having carried out the research, it appeared that complex sentence components of the subdialects are joined by conjunctions, relative adverbs, relative pronouns, particle ar and responsive words. Complex sentences might have one or several subordinate components. The sentences having one subordinate component might be of functional (explanatory, modifying, suppositional) and of semantic (time, reason, condition... [to full text]
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Briand, Bernard. "Caractérisation des métamagmatites en domaine profond et reconnaissance des sites géotechtoniques exemple du complexe Leptyno-Amphibolique de la Vallée du Lot, Massif-Central français." Grenoble 2 : ANRT, 1986. http://catalogue.bnf.fr/ark:/12148/cb37596436m.
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Motuzienė, Violeta. "Complex analysis of the influence of glazing on energy demand of public buildings." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101228_125700-05528.
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Applying building energy simulations, the dissertation investigates the issu-es of energy efficiency of public buildings. The main object of research – influence of the glazing of the public building on its energy demand. Next to its main function to ensure daylighting, glazing must to be energy efficient. So these requirements conflict.
The aim of this dissertation is to perform the complex analysis of the influence of glazing characteristics on the building‘s microclimate and lighting systems energy demand, taking into account DL demand. Also define, what glazing characteristics in Lithuanian and similar climate countries, are able to decrease buildings energy demand to the level of the low energy building. The paper approaches two major tasks. Task 1 is performance of the detailed analysis of influence of glazing on energy demand of the public building. Task 2 is determination of the efficient glazing characteristics of the energy efficient building.
The dissertation consists of Introduction, 4 chapters, Conclusions, references and list of published works of the author on the topic of dissertation.
The introduction reveals the investigated problem, importance of the thesis and the object of research and describes the purpose and tasks of the paper, research methodology, scientific novelty, the practical significance of results examined in the paper and defended statements. The introduction ends in presenting the author’s publications on the subject of the defended... [to full text]Disertacijoje, taikant dinaminį pastatų energijos poreikių modeliavimą, nag-rinėjamos viešosios paskirties pastatų energinio efektyvumo problemos. Pagrin-dinis tyrimo objektas yra viešosios paskirties pastato fasado įstiklinimo įtaka energijos poreikiams. Greta savo pagrindinės funkcijos – pakankamo natūralaus apšvietimo užtikrinimo, fasado įstiklinimas privalo būti energiškai efektyvus. Tai prieštaringi reikalavimai. Pagrindinis disertacijos tikslas – įvertinant natūralaus apšvietimo poreikį, kompleksiškai išanalizuoti viešosios paskirties pastato įstiklinimo charakteristi-kų įtaką pastato mikroklimato ir apšvietimo sistemų energijos poreikiams bei nustatyti, kokioms pastato įstiklinimo charakteristikoms esant, Lietuvoje bei pa-našaus klimato šalyse pastato energijos poreikius galima būtų sumažinti iki ma-žai energijos vartojančio pastato lygio. Darbe sprendžiami du pagrindiniai užda-viniai: pirmasis – atliekama įstiklinimo įtakos kondicionuojamo pastato energijos poreikiams analizė; antrasis – nustatomos efektyvios energiškai efek-tyvaus pastato įstiklinimo charakteristikos. Disertaciją sudaro įvadas, keturi skyriai, rezultatų apibendrinimas, naudotos literatūros ir autoriaus publikacijų disertacijos tema sąrašai. Įvadiniame skyriuje aptariama tiriamoji problema, darbo aktualumas, apra-šomas tyrimų objektas, formuluojamas darbo tikslas bei uždaviniai, aprašoma tyrimų metodika, darbo mokslinis naujumas, darbo rezultatų praktinė reikšmė, ginamieji teiginiai, pristatomos... [toliau žr. visą tekstą]
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Šliužas, Pranas. "Paslaugų kokybės gerinimas viešojo maitinimo sferoje." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2009~D_20140626_184520-53805.
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Vartotojų aptarnavimo kokybės gerinimas paslaugų sferoje yra labai svarbus dalykas kiekvienai šiuolaikines rinkos įmonei. Bet kuri įmonė siekia pelno, ir dalies paslaugų rinkos, dėl to yra priversti vykdyti savo mikro ir makro aplinkos bei marketingo komplekso elementų analizę. Kadangi daugelis paslaugų įmonių yra mažos, jos neturi išteklių, reikalingų siekiant pagerinti klientų aptarnavimo kokybę, ko pasekoje, prarandamas potencialius klientus, kas vėliau labai ryškiai atsispindi jos finansiniuose pasiekimuose. Mūsų tyrimui suteikia svarbą ir tai, kad paslaugų veikla Lietuvoje yra pakankamai jauna, todėl ne iki galo išanalizuota, trūksta praktinio pritaikymo paslaugų rinkoje.Consumer service quality improvement in services is a very relevant thing for each of the modern market company. Any firm seeking a profit, and part of the service market is forced to go to their micro and macro environments, analysis of marketing, which is one of the most important elements of people, or otherwise - by consumers. As many service firms are small, they are not the resources needed to improve the quality of customer service resulting in a loss of potential customers, who then very sharply reflected in the financing of its achievements. Our investigation gives relevance and the fact that as marketing activities, including market research and concept, Lithuania is young enough, and therefore not fully analyzed, and is limited to the practical use of the service companies.
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Kawamura, Márcio Seiti. "Dimensionamento e sequenciamento de lotes de produção na indústria de bens de consumo de higiene pessoal." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/3/3136/tde-30052012-114954/.
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O presente trabalho trata do problema integrado de dimensionamento e sequenciamento de lotes de produção. O processo de dimensionar e sequenciar lotes de produção consiste em determinar quanto produzir de cada produto e a sequência de produção desses lotes em cada máquina a cada período a fim de atender a uma demanda prevista sob as condições e capacidades operacionais existentes. O caso estudado nesse trabalho aborda o cenário de uma empresa de grande porte da indústria de bens de consumo de higiene pessoal, um mercado bastante concorrido no qual o bom nível de serviço de atendimento e a gestão de custos mostram-se essenciais na competição pelos clientes. Nessa empresa, existe um ambiente operacional complexo, composto por máquinas distintas em paralelo com capacidade limitada de produção cujos tempos de preparação (setup) são dependentes da sequência de produção, além de uma restrição de capacidade de armazenagem dos produtos fabricados, característica não encontrada na literatura existente. Os clientes permitem que ocorram atrasos de atendimento da demanda, porém isso é extremamente indesejável. Esse tipo de problema é NP-difícil, sendo geralmente tratado na literatura por heurísticas. Nesse trabalho, elaboramos nove diferentes estratégias de resolução baseadas na heurística relax-and-fix. O objetivo é, não somente resolver um problema real complexo, como também avaliar se o modo de articionamento e a sequência de resolução dos subproblemas influencia no desempenho da heurística. Os testes computacionais foram conduzidos em instâncias geradas aleatoriamente e em casos reais. Os resultados mostraram um bom desempenho e robustez da abordagem proposta. Técnicas alternativas foram aplicadas na estratégia com os melhores resultados para potencializar seu desempenho.This work adresses the integrated lot sizing and scheduling problem. The process of lot sizing and scheduling consists of determining how much to produce of each product and the scheduling of these lots in each machine in order to meet the demand under existing restrictions and operational capabilities. The case studied in this work describes the scenario of a big company in the industry of consumer goods for personal hygiene, a very competitive market in which the good service level for customers and the cost management show up in the competition for the clients. In this company, there is a complex operational environment, composed of distinct parallel machines with limited production capacity and sequence dependente setup times. There is also a limited finished goods storage capacity, a characteristic not found in the existing literature. Backordering is allowed but it is extremely undesirable. This problem is NP-hard and it has been treated by heuristics in the literature. In this work, we developed nine different solving strategies based on the relax-and-fix heuristics. The aim of this approach is not only to solve a complex real problem but also assess whether the form of partitioning and the sequence of solving the subproblems influences the performance of the relax-and-fix heuristics. The computational experiments were conducted on ramdomly generated instances and real problems. The results showed the good performance and the robustness of the proposed approach. Alternative techniques were applied in the strategy with the best results in the previous tests to enhance its performance.
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Salnikaitė, Violeta. "UAB Baltic Express rinkodaros komplekso tobulinimo galimybės." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2006. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2006~D_20061214_141434-41828.
Full textAbstract:
Magistro baigiamajame darbe analizuojamas UAB Baltic Express rinkodaros kompleksas ir jo tobulinimo galimybės. UAB Baltic Express yra logistinė įmonė teikianti transportavimo, sandėliavimo, krovinių draudimo, 3PL (trečiosios šalies logistika) paslaugas. Konkurencingoje logistikos rinkoje svarbus vaidmuo tenka įmonės rinkodarai, kuris vaidina svarbų vaidmenį įmonės vadyboje. Darbą sudaro trys pagrindinės dalys: Paslaugų rinkodaros komplekso elementų ypatumai, UAB Baltic Express rinkodaros komplekso įvertinimas ir UAB Baltic Express rinkodaros komplekso tobulinimo kryptys. Pirmąją dalį sudaro du skyriai, kuriuose detaliai analizuojama rinkodaros ir paslaugų rinkodaros komplekso definicija ir turinys ir rinkodaros komplekso elementų pagrindiniai aspektai.The MA paper analyses the marketing complex of Co. Ltd. Baltic Express and the possibilities to develop it. Co. Ltd. Baltic Express is a logistic company providing services of transportation, storage, insurance of cargos, 3PL (logistic services of the third company). In the competitive market of logistics, the major role falls on the marketing system of the company, which has an important position in the management process of the company. The current thesis consists of three major parts: The peculiarities of elements in the service marketing complex, Evaluation of Co. Ltd. Baltic Express marketing complex and the directions of developing the marketing complex of Co. Ltd. Baltic Express. The first part consists of two chapters, where a detailed analysis on the marketing, the definition of the service marketing complex, content and major aspects of marketing complex elements are expressed. The most extensive and exhaustive is the second part of the MA paper – where the evaluation of Co. Ltd. Baltic Express marketing complex is given and compiles 8 chapters. The Second chapter of the second part of MA paper presents the Co. Ltd. Baltic Express. The organizational structure of the company is introduced. The major attention is paid to the second chapter of the second part of the thesis – the evaluation of Co. Ltd. Baltic Express marketing complex analysis – where the results of the respondents analysis are proposed, the services of the company are evaluated, the strategy of... [to full text]
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Voronkevič, Alicija. "Tėvų ir specialistų bendradarbiavimas ugdant vaikus su kompleksine negalia." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2005. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2005~D_20050615_191116-20659.
Full textAbstract:
The following MA written work is about the cooperation between the parents and the specialists (the working staff of the educational institution), educating the students of the age from 12 till 21 years old with complex disability. The students with the complex disability are known as the students with many intellectual, hearing, visual, body and position, speech and communication disorders. During the educational process of the students with complex disability, of the age from 12 to 21 years old, many difficulties are coming up. They are: the difficulty to estimate the level of their perception, the difficulty to adapt the programs, the difficulty to fix the impact of the program on the child’s development.
The fist step educating these students is to realize their physical and intellectual abilities, their state of health and the needs of their family. The working staff of the educational institution and the parents of the students with complex disability creating and putting into practice in their everyday life an education plan, come to realize that working together in a team is an effective way to achieve the goals set in the education plan. The parents need to be motivated to become active participants, to be open to explain what kind of assistance they expect, what kind of help they need.
Here are the conclusions made according to the results of the research:
§ It became clear, that the specialists are cooperating with the parents of the students with complex... [to full text]
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Uljanionok, Julija. "Role of Cu(I) complexes in the electrochemical reduction of glycinate and maleate Cu(II) complexes." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20100204_100357-81350.
Full textAbstract:
A comparative investigation of electrochemical characteristics of two complex systems, viz. Cu|Cu(II), glycine and Cu|Cu(II), maleic acid, was carried out. The equations were obtained for quantitative description of pH-metric and spectrophotometric data, which were used for determination of equilibrium characteristics in Cu(II)-maleic acid solutions. Thermodynamic analysis shows that deep changes are possible in this system resulting in 90 % transform of Cu(II) into Cu(I). The rates of Cu corrosion and Cu2O formation are estimated to be of the same order (nmol cm-2 s-1). Regularities of formal electrochemical kinetics, which account for the mass transport of chemically interacting particles and for step-wise charge transfer process, are suitable for interpretation of voltammetric data of the Cu|Cu(II), glycine system. Kinetic parameters Cu(II) glycinate complex depend on the nature of the supporting electrolyte: the exchange current density decreases and the cathodic charge transfer coefficient increases in the sequence: Li+ - Na+ - K+ - Cs+. To enhance the Cu(I) generation in maleic acid system, the pre-electrolysis procedure was applied. It was found that its effect depends on solution pH. Applied theoretical model describes satisfactorily the steady-state voltammetric characteristics of Cu|Cu(II), maleic acid system, but some contradictory results were obtained in the case of time-dependent processes. Theoretical and experimental problems to be solved are discussed.Atliktas palyginamasis dviejų kompleksinių sistemų - Cu|Cu(II), glicinas ir Cu|Cu(II), maleino rūgštis - elektrocheminių charakteristikų tyrimas. Nustatytos maleino rūgšties tirpalų pusiausvyrinės charakteristikos. Kiekybiniam titravimo kreivių aprašymui išvestos lygtys, kuriose įvertinti medžiagų bei krūvių balansai bei atsižvelgta į praskiedimo efektus. Jų taikymas pH-metrinių duomenų analizei davė tokias maleato anijonų protonizacijos konstantų reikšmes: log = 6,05, log = 7,48. Nustatyta, kad tirpaluose su 0,3 M K2SO4 priedu protonizuotų ligando formų stabilumas sumažėja (log = 5,75, log = 7,30). Cu(II) maleatinių kompleksų stabilumui nustatyti panaudotas spektrofotometrijos metodas ir pasiūlyta duomenų analizės procedūra. Ji remiasi kiekybiniu absorbcijos spektrų aprašymu, taikant lygtis, išplaukiančias iš valdomo harmoninio osciliatoriaus teorijos. Išanalizavus įvairių sudėčių tirpalų absorbcijos maksimumo dydžius, prieita išvados, kad rūgščiose terpėse vyrauja monoligandinis kompleksas, kurio koncentracinė stabilumo konstanta log b1 = 2,2. Atlikta sistemos Cu|Cu(II), maleino rūgštis pusiausvyrų termodinaminė analizė. Nustatyta, kad esant metalinio vario ir tirpalų sąlyčiui, sistemoje galimi gilūs virsmai, kurių metu iki 90 % Cu(II) transformuojasi į Cu(I). Teorines išvadas patvirtina eksperimentiniai duomenys, gauti spektrofotometrijos ir elektrocheminės kvarco kristalo mikrogravimetrijos metodais. Įvertinti Cu korozijos bei fazinių Cu2O sluoksnių susidarymo... [toliau žr. visą tekstą]
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Petrėtienė, Erika. "Nusikaltimų aukų pagalbos ir paramos sistema Lietuvoje." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2009. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2008~D_20090122_111632-20985.
Full textAbstract:
Magistro baigiamajame darbe „Nusikaltimų aukų pagalbos ir paramos sistema Lietuvoje“ nagrinėjama Lietuvoje egzistuojanti pagalbos ir paramos nusikaltimų aukoms sistema, jos sudedamosios dalys ir elementai. Tema yra mažai nagrinėta ir aktuali tuo, kad tai naujas socialinių paslaugų sektorius Lietuvoje, kadangi iš esmės tuo susirūpinta vos prieš dešimtmetį, todėl dar nėra atlikta šios sistemos kompleksinė analizė, nėra tyrimų, kitų išsamesnių apibendrinančių darbų. Šio baigiamojo darbo objektas – nusikaltimų aukų pagalbos ir paramos sistema Lietuvoje, tikslas – pateikti rekomendacijas sistemai patobulinti. Siekiant šio tikslo buvo sprendžiami tokie uždaviniai kaip, valstybinių įstaigų bei nevyriausybinių organizacijų veiklos pagalbos nusikaltimų aukoms srityje nagrinėjimas ir vertinimas, pagalbos ir paramos nusikaltimo aukai sistemos Lietuvoje turinio atskleidimas, remiantis teisine literatūra ir teisės aktais, statistiniais duomenimis, internetinių svetainių informacija bei kitais informacijos šaltiniais, kai kurių Europos Sąjungos valstybių narių pagalbos ir paramos sistemos nagrinėjimas, pagalbos teikimo labiausiai pažeidžiamoms nusikaltimų aukoms – moterims ir vaikams analizė ir vertinimas, remiantis ekspertų apklausa. Atsižvelgiant į tyrimo objektą ir tikslą, magistro darbe buvo analizuojami tarptautiniai ir nacionaliniai teisės aktai, reglamentuojantys nusikaltimų aukų apsaugą, taip pat buvo vertinama institucijų ir organizacijų veikla šioje srityje, remiantis įvairiais... [toliau žr. visą tekstą]In Master’s final thesis „The System of Help and Support for Crime Victims in Lithuania“ there is researched an existing in Lithuania system of help and support for crime victims, its components and elements. This theme has not been widely researched and is relevant because it is a new sector of social services in Lithuania. For the reason that this issue was raised only ten years ago there is no complex analysis of this system; there are no researches and other comprehensive resumptive works. The object of this final thesis is the system of help and support for crime victims in Lithuania; its purpose is to give recommendations for the improvement of the system. For the attainment of the purpose there were set the following tasks: the research and evaluation of the activity of public companies and nongovernmental organizations in the field of help and support for crime victims; the revelation of the content of system of help and support for crime victims in Lithuania on the ground of juridical literature, legal acts, data, information of Internet pages and other information sources; the analysis of some help and support systems of the member states of the European Union; the analysis and evaluation of help and support provided to the most vulnerable crime victims – women and children on the ground of the inquiry of experts. Considering the object and purpose of the research in this Master’s thesis there were analyzed international and national legal acts regulating the... [to full text]
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Gaidamauskaitė, Evelina. "Kompiuterinis daugiapakopių reakcijų kinetikos biojutikliuose modeliavimas." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2011. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20111122_102635-72506.
Full textAbstract:
Biojutikliai yra analitiniai įtaisai sudaryti iš biologiškai aktyvios bei selektyviai atpažįstančios substratą medžiagos, dažniausiai fermento, ir keitiklio formuojančio makroskopinį fizinį signalą. Naujų įtaisų kūrimui būtini lygiagretūs eksperimentiniai tyrimai. Skaitiniai eksperimentai gali patikimai pakeisti fizinius. Modeliuojant tokius biojutiklius, būtina atsižvelgti į juose vykstančių procesų daugiapakopį pobūdį. Šiame darbe nuodugniai ištirtos tokių reakcijų schemų savybės. Sudaryti originalūs matematiniai modeliai optiniam peroksidaziniam bei amperometriniam lakaziniam daugiapakopiams biojutikliams. Deterministinė modelių sudarymo proceso prigimtis leidžia jį automatizuoti. Remiantis šiuo principu sukurtas bendras įrankis kompiuteriniam daugiapakopių biojutiklių modeliavimui. Siekiant optimizuoti skaitinį sprendimą palygintos dažniausiai naudojamos baigtinių skirtumų skaitinio sprendimo schemos sprendžiant reakcijos - difuzijos lygtis. Pastarasis palyginimas parodė, kad greičiausiai reikiamas sprendinio tikslumas pasiekiamas taikant neišreikštinę bei Hopscotch schemas. Uždaviniams, kuriems sparta svarbesnė už tikslumą, turėtų būti taikoma išreikštinė schema. Taikant naują įrankį atliktas kompiuterinis daugiapakopių biojutiklių modeliavimas. Kompiuterinis lakazinio biojutiklio modeliavimas teoriškai paaiškino eksperimentiškai stebėtą sinergetinę mediatoriaus įtaką biojutiklio atsakui. Peroksidazinio biojutiklio kompiuterinio modeliavimo rezultatai parodė, kad plataus... [toliau žr. visą tekstą]Biosensors are analytical devices made up of a combination of a biological entity, usually an enzyme, that recognizes a specific analyte (substrate) and the transducer that translates the biorecognition event into a signal. In order to create new types of biosensors the corresponding experimental studies are necessary. Computational experiments could very well replace very expensive physical ones. However, the multi-step character of a chemical reaction scheme must be considered and modeled accordingly. In this thesis such reaction schemes were studied in great details. Original mathematical models were developed for optical peroxidase-based and amperometric laccase-based biosensors. The deterministic nature of model construction allows the automated models to be built. Based on this assumption flexible model for computational modeling of different practical multistep biosensors was developed. In order to optimize the numerical solution of the reaction-diffusion type equations common finite difference schemes were compared. The comparison shows that the fastest schemes to achieve the required relative error are implicit and Hopscotch schemes. For the problems where accuracy is not a significant factor but the speed is, the simplest explicit scheme should be used. Applying the new flexible model a computational modeling of the multi-step biosensors were produced. The modeling of laccase biosensor explained and confirmed the synergistic effect. The computational modeling of the... [to full text]
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Ambrozaitytė, Laima. "Determination of genomic variants of the complex aetiology cleft lip and (or) palate in Lithuanian patient group." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2011. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20110628_134730-72245.
Full textAbstract:
The incidence of cleft lip and (or) palate (CL/P) varies from 0.4 to 2.0 in 1000 live births across populations. More and more CL/P candidate loci are being confirmed using novel genome-wide methods of molecular genetics and tools of statistical analysis. The aim of this study was to identify the alleles of the candidate genes for cleft lip with or without cleft palate and isolated cleft palate in the Lithuanian patient group, applying the molecular genotyping of the genomic markers in 42 CL/P candidate genes and association analysis using transmission disequilibrium test approach. Using families’ studies and case – control studies approach CL/P candidate genes TIMP2, BMP2, FN1 variants were confirmed for cleft lip with or without cleft palate and COL11A1, COL11A2, COL2A1 for cleft palate only in the population of Lithuania. Estimating the association analysis results of the CL/P patients of Lithuania, Latvia and Estonia, FGF1, TIMP2 were identified as candidate genes for cleft lip with or without cleft palate and COL2A1, COL11A2 for isolated cleft palate in the North East European populations. Different genomic risk variants have influence for cleft lip with or without cleft palate and for cleft palate only, which confirms the hypothesis of different mechanisms for these two phenotypes.Kaukolės veidinės dalies anomalijos, ypač lūpos ir (arba) gomurio nesuaugimai (L/GN), yra vienos dažniausių žmogaus įgimtų ydų. Įvairių autorių duomenimis jų dažnumas svyruoja nuo 0,4 iki 2,0 1000 gimusiųjų. Sparčiai tobulėjant molekulinės genetikos metodams ir statistinės analizės įrankiams vis daugiau kandidatinių L/GN genetinių sričių identifikuojama plataus masto viso genomo tyrimais. Šio darbo tikslas buvo nustatyti genų kandidatų alelius, lemiančius polinkį (nesindrominiam) lūpos nesuaugimui su arba be gomurio nesuaugimo ir izoliuotam gomurio nesuaugimui Lietuvos pacientų grupėje, tiriant 42–jų genų galimai lemiančių L/GN genominius žymenis molekuliniais genetiniais metodais ir taikant statistinę asociacijos analizę nepusiausviro perdavimo testu. Taikant šeimų tyrimo ir atvejo – kontrolės tyrimo asociacijos analizės strategijas pacientų su lūpos nesuaugimu su arba be gomurio nesuaugimo grupėje, patvirtinti TIMP2, BMP2, FN1 genų kandidatų variantai lemiantys lūpos nesuaugimus su arba be gomurio nesuaugimo ir gomurio nesuaugimus Lietuvos populiacijoje, COL11A1, COL11A2, COL2A1 genų kandidatų variantai lemiantys gomurio nesuaugimus Lietuvos populiacijoje. Įvertinus Lietuvos, Latvijos ir Estijos pacientų su L/GN asociacijos analizės rezultatus, buvo nustatyta, kad Šiaurės Rytų Europos populiacijose polinkį lūpos nesuaugimui su arba be gomurio nesuaugimo lemia FGF1, TIMP2 genų variantai ir polinkį gomurio nesuaugimui COL2A1, COL11A2 genų variantai. Lūpos nesuaugimus su arba... [toliau žr. visą tekstą]
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Ruseckas, Jonas. "Daugiabučių namų rekonstrukcija." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2009. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2009~D_20090702_141958-38900.
Full textAbstract:
Baigiamajame magistro darbe nagrinėjamos rekonstrukcijos atsiradimo priežastys, kompleksinės daugiabučių gyvenamųjų namų rekonstrukcijos problematika. Tyrimo objektas – gyvenamųjų namų ir juos supančios aplinkos rekonstrukcija. Šiuo metu gyvenamųjų namų modernizavimas vykdomas tvarkant atskirus pastatus ir pagrinde termodinaminiu požiūriu. Darbe tyrimo objektas išplečiamas iki kvartalo (mikrorajono) apibūdinant tai kompleksine rekonstrukcija. Analizuojamos pagrindinių sąvokų - renovacija, rekonstrukcija, kapitaliai renovuojamas pastatas, reikšmės. Pabrėžiami pagrindiniai kompleksinės rekonstrukcijos tyrimo ir vykdymo etapai. Aptariamos statinio statybos rūšys ir formos rekonstrukcijos vykdymui įgyvendinti. Darbą sudaro 5 dalys: įvadas, du skyriai, išvados, literatūros sąrašas. Darbo apimtis – 60 p. teksto be priedų, 17 iliustr., 5 schemos, 2 lent., 41 bibliografinis šaltinis.The final master’s paper deals with the reasons of the origin of reconstruction as well as with the problematics of complex reconstruction of apartment houses. Subject of the research is reconstruction of dwelling houses and surrounding environment thereof. For the time being, modernisation of dwelling houses is carried out through reconstruction of individual buildings, basically in terms of thermodynamics. In the paper, the subject of the research is evolved into a quarter (microdistrict) characterising this by complex reconstruction. The meanings of the main concepts, i.e. renovation, reconstruction, building under major renovation, are analysed. The main stages of complex reconstruction research and implementation are highlighted. Building construction types and forms for implementation of reconstruction are discussed. The paper consists of the following 5 parts: introduction, two sections, conclusions, list of references. Size of the paper contains 60 pages of text without annexes, 17 illustrations, 5 diagrams, 2 tables, 41 references.
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Callipo, Manuela. "Pour l’histoire du « discours bien construit » : le livre I du De contructione d’Apollonius Dyscole et les reprises chez Priscien." Thesis, Lille 3, 2013. http://www.theses.fr/2013LIL30015.
Full textAbstract:
Le sujet de ce travail, c'est le De constructione d'Apollonios Dyscole, le premier traité de syntaxe de l'antiquité, et en particulier son premier livre, étudié par rapport au livre XVII de constructione des Institutions grammaticae de Priscien, qui s'est inspiré d'Apollonios au début du VIe siècle ap. J.-C. Le texte grec, imprimé selon l'édition réalisée par G. Uhlig en 1910, à l'occurence modifiée à cause de choix ecdotiques différents de ceux du philologue allemand, est suivi par sa première traduction en langue italienne ; de plus, pour permettre une comparaison avec le traité latin, on a imprimé et traduit en italien les passages correspondants du livre XVII de Priscien. Le commentaire a pour but d'expliquer les choix critiques, mais une attention particulière est aussi dédiée à l'ordre des parties du discours établi par Apollonios et à leurs rapports avec la tradition grammaticale grecque et latine, même sur papyrus. En ce qui concerne Priscien, on a transcrit - pour les parties traduites - le manuscrit Vaticanus lat. 3313, qui permet d'améliorer, ne fût-Ce que dans le détail, le texte de l'édition de Hertz (1855-1859). Textes et traductions sont précédés par une introduction qui, après un excursus sur la méthode analogique d'Apollonios, examine les rapports entre grammaire et philosophie, en particulier stoïcienne et platonicienne, à fin de montrer qu'il n'est pas possible de corriger Apollonios par Priscien et Priscien par Apollonios : l'arrière-Plan néoplatonicien de Prisicen ne semble pas agir chez Apollonios, qui vécut quatre siècles avant. Enfin, on a retracé l'histoire de la tradition manuscrite et des éditions modernes des deux traités De constructioneThe subject of this research is the De constructione by Apollonius Dyscolus, the first treatise on syntax written in classical antiquity, and in particular its first book, studied in comparison with the book XVII de constructione of the Institutiones grammaticae by Priscian, who looked back to Apollonius at the beginning of the VI century. The Greek text, printed according to the edition by G. Uhlig (1910), sometimes modifed because of different textual choices, is followed by its first Italian translation ; furthermore, to allow a comparison with the latin treatise, the corresponding passages of the book XVII by Priscian have been printed and translated too. The commentary aims to explain the critical choices, but particular attention has also been paid to the order of the parts of speech set by Apollonius and to their relationship with Greek and latin grammatical tradition, also on papyrus. With respect to the translated sections of Priscian, a new manuscript, the Vaticanus lat. 3313, has been transcribed ; this made it possible to improve, although only in the details, the text of the edition by Hertz (1855-1859). Texts and translations are preceded by an introduction which, after an excursus about Apollonius' analogical method, analyses the relationship between grammar and philosophy, especially Stoicism and Platonicism, to show that it is not possible to correct Apollonius through Priscian through Apollonius : Priscian's Neoplatonic background seems not to be active in Apollonius, who lived four centuries earlier. Finally, this work offers a short history of manuscript tradition and of modern editions of the two treatises De constructione
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Olšauskas, Martynas. "Sezoninis surenkamų konstrukcijų pramogų kompleksas." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2006. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2006~D_20060114_231340-60167.
Full textAbstract:
SUMMARY
Master’s Thesis “Equipment of Seasonal Entertainment Complex Made out of Portable Constructions” encompasses several objects. This is a work of complex design where a sense of ensemble and harmony is very important. While creating the parts of the complex, unfailing resources of bionic forms were the base for it. Improvised forms of animate nature enable to create unusual objects which have specific stylistics, tectonics, rhythmic and character. Exactly nature is the unfailing source of new ideas according to both functional and aesthetical points of view. Light constructions and dynamic bionic forms integrate the entertainment complex into a natural environment organically. Moreover, it functions as equipoise to hard and cold stylistics of blocks of flats. The complex is a seasonal one; that is why an idea of portable constructions was chosen. Thanks to this a seasonal and complex space for entertainment and recreation is formed.
Even an elementary object of architecture cannot be imagined without a structural constructional framework. In modern architecture, structure is one of the elements of plastic expression (Mogilnickas (1995) Techninės estetikos pradmenys / Basics of Technical Aesthetics [in Lithuanian]).
Portable metal constructions and modern textile architecture form an impression of lightness and provide the entertainment complex with an exceptional character.
When operating with the material and creating a material, functional form, professionalism... [to full text]
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Veliutė, Ingrida. "Kaunas Fortress Historical and Architectural Heritage and Its Animation Possibilities." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2012. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2012~D_20121122_154756-26186.
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Cultural heritage objects’ protection has always been closely related to country‘s social, economic and political changes. These changes influence heritage protection techniques and methodology, also stipulate heritage objects’ selection and protection priorities. Brutal and dissonant defence heritage of XIX-XX centuries was already perceived and absorbed in many European countries started being recognized in Lithuania. Basis of analysis is defence objects, administrative buildings, chapels, infrastructure and other Kaunas Fortress elements, built in 1882–1915, which have influenced changes in Kaunas city and its surroundings as well as the further city development. Kaunas fortress is presented as complex of various purposes objects, which were closely related to each other and could not function separately. Thesis analyses two fortification heritage changes process lines, occurred since construction to the present: the first – Kaunas Fortress complex and its separate components visual relation to environment; the second – Fortress complex objects and users relationship and utilization changes. Thesis is focusing more on military complex appearance, its ideological context, also on the development of social, economic and political factors during construction and defence time. This directly influences the creation of high-quality story and cultural heritage animation. Dissertation emphasizes Kaunas Fortress complex evaluation and acknowledgment as the object of heritage... [to full text]Paveldo objektų apsauga visada buvo ir išlieka glaudžiai susijusi su šalies socialiniais, ekonominiais ir politiniais pokyčiais. Šie pokyčiai įtakoja paveldo saugojimo būdus ir metodiką bei sąlygoja paveldo objektų selekciją ir apsaugos prioritetus. Ypatingoje situacijoje atsiduria disonuojantis, brutalus, daugelyje Europos šalių jau įsisąmonintas ir įsisavintas, o Lietuvoje dar tik pradedamas pripažinti gynybinis XIX – XX a. sandūros paveldas. Disertacijos analizės pagrindas yra 1882–1915 m. pastatyti Kauno tvirtovės gynybiniai objektai, administraciniai pastatai, maldos namai, infrastruktūra ir kiti tvirtovės elementai, turėję įtakos Kauno miesto ir apylinkių kraštovaizdžio pokyčiams ir tolesnei miesto plėtrai. Kauno tvirtovė nagrinėta kaip įvairios paskirties objektų kompleksas, kur visi elementai buvo glaudžiai tarpusavyje susiję ir negalėjo funkcionuoti atskirai vieni nuo kitų. Darbe analizuotos dvi fortifikacijos paveldo kaitos proceso kryptys, vykusiosos nuo statybos pradžios iki šių dienų: pirmoji – Kauno tvirtovės komplekso bei atskirų jos komponentų vizualinis ryšys su aplinka; antroji – tvirtovės komplekso objektų ir naudotojų tarpusavio ryšys bei panaudos kaita. Dėmesys koncentruojamas į karinio komplekso atsiradimo ideologinį kontekstą bei socialinių, ekonominių, politinių veiksnių plėtotę jo statybos ir gynybos metu, kas tiesiogiai daro įtaką kokybiško pasakojimo kūrimui ir paveldo animavimui. Disertacijoje išryškintas Kauno tvirtovės komplekso įvertinimo ir... [toliau žr. visą tekstą]
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Nabiyeva, Svetlana. "Water-based headspace single-drop microextraction and capillary electrophoresis for the determination of volatile inorganic compounds in complex matrices." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101214_103540-08331.
Full textAbstract:
The objective of the present work was systematic investigation of water-based headspace single-drop microextraction (SDME) technique, its conjunction with capillary electrophoresis, and application for the analysis of volatile inorganic compounds (cyanide and ammonium) in biomedical and environmental samples. Theoretically evaluated and experimentally confirmed that the manipulation in the volatility of the analyte by its chemical modification in the sample and/or in the acceptor phase is the most effective way to enhance the extraction performance in headspace SDME. Maximum extraction efficiency for cyanide was observed in the pH range 4.5–7.5, where cyanide anion is completely transferred into volatile HCN. Complete cyanide recoveries (≥96%) from metal-cyanide complexes were obtained by using two ligand-exchange reagents (ethylenediamine and dithizone). Maximum extraction efficiency was observed by the extraction of ammonia from alkaline samples (pH≥11) with neutral or slightly acidic acceptor phase (pH 3-7). Compared to existing methods water-based headspace SDME is significantly faster and simpler. In addition, proposed technique does not require any sample pre-treatment (deproteinization, acidic/alkaline distillation, etc.) and thus is much less susceptible to interferences. Finally, it offers possibility to choice of a wider variety of solvents and, consequently, enhances the range of extractable analytes as well as the range of analytical methods that can be coupled... [to full text]Daktaro disertacijoje nuodugniai ištirtas mikroekstrakcijos iš viršerdvės vandens lašu metodas, apjungtas su kapiliarine elektroforeze bei pritaikytas lakių neorganinių junginių (cianido ir amonio) koncentravimui ir nustatymui biomedicininiuose ir aplinkos objektuose. Teoriniais skaičiavimais parodyta ir eksperimentiškai patvirtinta, kad efektyviausias būdas mikroekstrakcijos iš viršerdvės efektyvumui pagerinti – analitės lakumo didinimas chemiškai modifikuojant ją prieš ekstrakciją ir/arba jos lakumo mažinimas modifikuojant ją akceptorinėje fazėje. Maksimalus cianido mikroekstrakcijos efektyvumas pasiekiamas pH srityje 4,5- 7,5, kur cianidas kiekybiškai pervedamas į lakų HCN. Ištyrus cianido mikroekstrakciją tirpiklio lašu iš metalų cianidinių kompleksų tirpalų nustatyta, kad kiekybiškas silpnai surišto cianido suardymas (cianido išgavos siekia ≥96%) pasiekiamas panaudojus ligandų mainus etilendiamino ir ditizono mišiniu. Maksimalus amonio mikroekstrakcijos efektyvumas pasiekiamas ekstrahuojant jį iš pašarmintų mėginių (pH≥11) akceptorine faze, kurios pH 3-7. Lyginant su standartiniais metodais, siūlomas metodas yra greitesnis ir paprastesnis, visiškai eliminuojama mėginio matricos įtaka, nereikalingos jokios drastiškos (deproteinizacija, veikimas rūgštimis/šarmais, distiliacija ir pan.) manipuliacijos su mėginiu, todėl išvengiama mėginio matricos įtakos. Mikroekstrakcija iš viršerdvės vandens lašu praplečia mikroekstrakcijos metodų taikymo sritis: atveriama galimybė labai... [toliau žr. visą tekstą]
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Grabliauskaitė, Aušra. "ES šiaurinio regiono vaidmuo įgyvendinant ES energetinį saugumą XXI amžiuje." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2009. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2009~D_20090612_103411-39900.
Full textAbstract:
Pagrindinis darbo tikslas yra išanalizuoti Europos Sąjungos šiaurinio regiono, akcentuojant Rusijos Federacijos ir Norvegijos Karalystės įtaką regione, vaidmenį, užtikrinant energetinį saugumą Europos Sąjungoje, bei numatyti galimas ES energetikos politikos perspektyvas netolimoje ateityje. Siekiant geriau atskleisti darbo tikslą, keliami šie uždaviniai: apibrėžti saugumo sąvokos pagrindines dimensijas, veiksnius bei regioninio saugumo komplekso svarbą ir reikšmę Europos Sąjungos kontekste; atskleisti Europos Sąjungos šiaurinio ir Arkties regionų vaidmenį ES energetikos politikoje; išanalizuoti dabartinę ES energetinę padėtį ir priklausomybę nuo energetinių išteklių importo; išsiaiškinti Rusijos Federacijos ir Norvegijos Karalystės vietą ES energetinio saugumo kontekste; numatyti ES kaip galimo energetinio saugumo komplekso energetikos politikos ateities gaires.
Parašius darbą pavyko patvirtinti iškeltą ginamąjį teiginį, jog nacionalinių valstybių kova dėl energetinių išteklių ir individualūs sprendimai sudaro sąlygas energetiniam nesaugumui Europos Sąjungoje, kadangi ES deklaruojamas siekis kalbėti „vienu balsu“ ir įgyvendinti bendrą energetinę politiką, prasilenkia su valstybių nacionaliniais interesais ir lieka neįgyvendintas. Nors Europos Sąjunga gali būti laikoma energetiniu saugumo kompleksu, kuriame energetinės priklausomybės santykis tarp šalių yra suvokiamas kaip grėsmė (kurios šaltinis iš esmės yra vienas – Rusijos Federacija), tačiau didėjantis vartojimas ir... [toliau žr. visą tekstą]The main object of this study is to analyze the role of the North region of European Union, ensuring energy security in the EU and foresee the possible perspectives of the EU’s energy policy in the nearest future. The North region is analyzed emphasizing two countries which are main energy suppliers for Europe – Russian Federation and Norway. For a better understanding of the object, the specific proposition has been formulated: the struggle of the national states for energy resources and individual decisions allow energy insecurity in the European Union since the EU’s declared objective „to speak in one voice“ is inconsistent with national interests of EU’s member states. Although the European Union can be defined as the complex of energy security in which the relation of dependence is comprehended as a threat (the main source of this threat is the Russian Federation’s energy monopoly), however the growing consumption and competition for energy resources encourage the countries to search for alternative energy resources and its suppliers. These actions disrupt the initiatives of the common European Union’s energy policy. The political consciousness is not yet grown in the EU’s countries and it determinates the weakness of the EU as a political construct in case of competition with the rising economical powers (such as India and China) for energy resources. On the other hand the consumption is growing in the Russian market as well, which implies possible insecurity of supply... [to full text]
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Jakutytė-Sungailienė, Asta. "Įmonė kaip civilinių teisių objektas." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101108_125841-35971.
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Šio tyrimo tikslas – išsamiai ir sistemiškai išanalizuoti įmonės, kaip civilinių teisių objekto, teisinę prigimtį bei civilinį apyvartumą, nustatyti aktualiausias teorines ir praktines šio instituto aiškinimo ir taikymo problemas ir, remiantis užsienio valstybių patirtimi bei pasaulinėmis tendencijomis, pateikti pasiūlymus ir rekomendacijas šioms problemoms spręsti bei esamam teisiniam reguliavimui tobulinti, taip papildant nacionalinės teisės doktriną šioje srityje.
Disertacijoje nagrinėjama problema aktuali tiek moksliniu, tiek praktiniu požiūriu, nes iki šiol Lietuvos teisės doktrinoje įmonės, kaip civilinių teisių objekto, specifika yra beveik nenagrinėta, išskyrus fragmentišką atskirų sandorių, kurių objektas yra įmonė, (pvz., įmonės pirkimą-pardavimą, įmonės įkeitimą) aptarimą. Šioje disertacijoje pirmą kartą Lietuvoje pateikiama visapusiška, nuodugni ir sisteminė įmonės, kaip civilinių teisių objekto, ypatumų analizė, todėl toks kompleksinis tyrimas, pirmiausia, yra reikšmingas teoriniu-doktrininiu požiūriu. Be to, šis tyrimas svarbus ir praktiniu požiūriu. Tyrimo metu išanalizuoti nacionaliniai ir užsienio valstybių teisės aktai, doktrina ir teismų praktika, susijusi su įmonės, kaip turtinio komplekso, teisinio statuso išskirtinumu bei sudaromų sandorių specifika. Šios analizės pagrindu suformuluoti praktiniai pasiūlymai Lietuvos įstatymų leidėjui, siekiant supaprastinti ir taip padidinti įmonės, kaip turtinio komplekso, civilinį apyvartumą (pvz., dėl kreditorių... [toliau žr. visą tekstą]The purpose of the research is thorough and systematic analysis of the legal nature and civil turnover of enterprise as an object of civil rights, identifying the most relevant theoretical and practical problems in interpretation and application of legal norms regarding enterprise, providing with suggestions and recommendations for resolution of identified problems and improvement of existing legal regulation according to the experience of foreign countries and contemporary global trends, and contributing to the national legal doctrine. The subject-matter of the research is relevant both, in scientific and in practical terms, as the peculiarities of enterprise as an object civil rights lacks thorough analysis in the legal doctrine of Lithuania. Apart from several narrow problem-orientated publications regarding separate transactions with enterprise (e. g. sale-purchase and mortgage of enterprise), until now the legal doctrine of Lithuania lacks sufficiently profound analysis of peculiarities of enterprise as an object of civil rights. For the first time in Lithuania this dissertation delivers a thorough and systematic analysis of peculiarities of enterprise as an object of civil rights, thus such integrated research, firstly, is relevant in theoretical terms. Moreover this research is also relevant to legal practice. During the research the legal acts, court practice and legal doctrine of Lithuania and foreign countries regarding the peculiar legal status and specific... [to full text]
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Kaziukonis, Laimis. "Vilniaus geležinkelio stoties renovacija." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2011. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20110622_155025-52152.
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Magistro studijų baigiamojo darbo tikslas – kompleksiškai spręsti sudėtingų transporto komunikacijų, urbanistinių struktūrų renovacijos problemas. Baigiamojo darbo analitinėje dalyje nagrinėjama geležinkelio stočių morfologinė raida, sandara ir funkciniai ryšiai. Remiantis metodine literatūra ir užsienio patirtimi suformuojamos pagrindinės urbanistinės geležinkelio stočių renovacijos tendencijos. Tiriamojoje darbo dalyje atliekama Vilniaus geležinkelio stoties istorinė ir urbanistinė analizė, bei pateikiami paminklosauginiai tvarkymo siūlymai ir rekomendacijos. Eksperimentinėje dalyje pateikti variantai vertinami pagal analitinės dalies išvadas. Privalumai ir trūkumai nagrinėjami pagal penkis pagrindinius kriterijus: tipologiją, infrastruktūrą, urbanistinę struktūrą, vidinę struktūrą ir istorinį kontekstą. Projektinėje baigiamojo darbo dalyje detalizuojamas stoties-parko variantas, atitinkantis šiuolaikinius sudėtingų transporto komunikacijų renovacijos metodus ir tendencijas, bei kompleksiškai sprendžiantis ne tik atskiro pastato, bet ir urbanistines teritorijos problemas.The main objective of Final work of Architecture master studies is to find solution for complex transport communication and urban renovation problems. Morphological development of railway station, structure and functional intercourse are analyzed in analytical part of Final work. Main tendencies of urban renovation of railway stations are formulated with reference to methodological literature and foreign experience. Proposals and recommendations for regulation of historical heritage are made after historical and urban analysis of Vilnius railway station in explorative part of Final work. In experimental part project proposals are evaluated by conclusions of analytical part. Strengths and weakness analyzed by five main criteria: morphology, infrastructure, urban structure, plan and historical context. Version of station-park is developed in Final work project part. It corresponds to contemporary transport communication renovation methods and tendencies and offers complex solution not only for unique building problems, but for whole urban territory complex.
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Šiupšinskas, Laimonas. "Kompleksinis studentų fizinio aktyvumo vertinimas fizinės sveikatos, fizinio pajėgumo ir kūno masės kompozicijos rodikliais." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2007. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2006~D_20070102_132605-43549.
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Physical activity level of students is decreasing. Students are specific population group with similar patterns of habitual physical activity influenced by study process. That formed requirement to search for a new ways to assess physical activity of the students indirectly. Offered method assesses level of physical health, measures physical fitness and evaluates body composition. The aim of the study is to evaluate indirectly measured health-enhanced physical activity of the students by physical health, physical fitness and body composition parameters. Objectives: 1) To evaluate student’s physical development and physiological parameters related to physical health. 2) To measure student’s physical fitness and analyze its changes in 2003/2004 – 2005/2006 academic years. 3) To evaluate peculiarities of body composition and its components. 4) To propose complex evaluation methodology for indirect measured physical activity and to determine student’s level of physical health, physical fitness and body composition. According G. Apanasenka scoring system student’s physical health was lower than average. Physical fitness of males tested by Eurofit tests was in the average. Female’s physical fitness results were lowest of physical activity related components. Majority of assessed students (56% of males and 67% of females) had normal body weight. It was proposed method for the complex evaluation of indirectly measured physical activity and its components: physical health, physical... [to full text]
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Povilaitytė, Vaida. "Edukacinių paslaugų marketingo komplekso formavimo modelis." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2008. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2008~D_20080925_114955-67255.
Full textAbstract:
Darbe palyginami pagrindiniai marketingo modeliai, nagrinėjama konkurencingumo problema bei marketingo įrankių panaudojimo galimybės veiklos tobulinimui. Analizuojamos mokymo paslaugų ypatybės, aukštųjų mokyklų orientavimosi į rinką stadijos, tikslinės rinkos ir atskirų vartotojų grupių poreikiai. Akcentuojama, jog esminė edukacinių paslaugų ypatybė yra ta, kad egzistuoja kelios vartotojų grupės, kurios turi esamų ir potencialių interesų: tai ne tik studentai, bet ir jų tėvai, darbdaviai, vyriausybinės organizacijos, aukštosios mokyklos darbuotojai ir t.t. Kiekviena iš šių grupių sudaro mokyklos tikslinę rinką ir joms reikia sukurti ir taikyti skirtingą marketingo priemonių kompleksą. Siūloma atsižvelgti ir į studentų kaip mokymo paslaugų vartotojų poreikių diferenciaciją. Įvertinus ŠU Edukologijos fakulteto studentų lūkesčių patenkinimo lygį, siūlomos esamos marketingo komplekso tobulinimo kryptys, užtikrinant šio proceso tęstinumą bei vykdymą kiekviename aukštosios mokyklos lygyje. Pateikiamas edukacinių paslaugų marketingo komplekso formavimo modelis.In the present thesis, there are compared the main marketing models, considered the problem of competitive ability and possibilities to use marketing tools for activity improvement. The peculiarities of education services, the stages of market orientation of higher education schools, purpose markets and the needs of different groups of consumers are analysed. It is emphasised that the essential peculiarity of educational services is existence of several consumer groups, which have their existing and potential interests: these are not only students, however, also their parents, employers, governmental organisations, employees of higher education schools and etc. Each of these groups makes a purpose market of a school and they need different sets of marketing tools to be developed and applied. Having assessed the level of satisfaction of expectations of the students of Šiauliai University Educology Department, the trends and directions to improve existing marketing complex are proposed ensuring the continuity of the process and its performance on each level of a higher education school. The model of formation of educational service marketing complex is provided herein.
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Kazlauskienė, Eglė. "Metalo kompleksinių dažiklių sorbcija jonitais." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2012. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2012~D_20120131_092423-21579.
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Pagrindinis aplinkosauginės politikos bruožas yra taršos metalo dažikliais mažinimas gamybos metu ir efektyvių šalinimo iš nuotekų metodų paieška. Regeneruojami, mechaniškai atsparūs ir nekenksmingi gamtai polimeriniai sorbentai naudojami dažikliams valyti yra pranašesni už plačiai paplitusias aktyvintąsias anglis. Ištyrus polimerinių sorbentų sorbcines ir regeneracines savybes, nustačius palankias darbines sąlygas, sorbentai gali būti pritaikomi nuotekų valymui, o išvalytas vanduo grąžinamas atgal į gamybinius procesus. Tokiu būdu, vandens ištekliai gali būti apsaugoti nuo taršos sunkiaisiais metalais ir organiniais junginiais, sumažinti gamybos kaštai, nes regeneruoti sorbentai naudojami pakartotinai.
Šiame darbe tiriama metalo kompleksinių dažiklių Navy M-DNL (1:2 chromo monoazo kompleksas) (Navy) ir Acid Blue 249 (Vario (II) ftalocianino tetrasulforūgšties tetranatrio druska) (CuPc) sorbcijos sintetiniais jonitais dėsningumus statinėmis sąlygomis ir įvertinti jonitų galimybes valyti nuotekas nuo dažiklių dinaminėmis sąlygomis.
Geriausiai iš vandeninių tirpalų šalinantys dažiklius sorbentai atrinkti pagal geriausią sorbcinė gebą ir regeneraciją, dažiklio pasiskirstymo tirpale koeficientą yra: silpnai baziniai anijonitai Purolite A 847 (A 845), stipriai bazinis anijonitas Purolite A 500PS, nejoninis makrotinklinis sorbentas Macronet MN 200. Statinėmis sąlygomis nustatyta dažiklių (Navy ir CuPc) sorbcijai palankiausia terpė (pH 2) ir temperatūra atitinkamai dažikliui Navy... [toliau žr. visą tekstą]Metal complex dyes are a problematic group of substances, present in the negatively charged anion form in mixed industrial wastewater that should be removed. Therefore, a complete removal of these hazardous dyes from wastewaters is necessary to prevent them from release into the environment. Adsorption on activated carbon of dyes has been investigated widely. However some disadvantages using activated carbon in practice were observed, e.g. high regeneration costs and production of fines due to the brittle nature. Since their adsorption capacities, mechanical strength, and other properties need further improvement for wider application, the polymeric sorbents are still under development as a potential alternative to activated carbons. The main aim of the present work was to investigate absorption regularity metal complex Lanasyl Navy M-DNL and Acid Blue 249 (copper (II) phthalocyanine) dyes on synthetic ion exchangers under static conditions and evaluate facility of ion exchangers to removal dyes from wastewaters by dynamic conditions. Screening with chromium-complex dye (acid brown NKM, Lanasyn Navy M-DNL) solutions wide range sorbents, according to the physical chemical characteristics of sorbents and established dye removal parameters (sorption capacity, distribution coefficient and sorbent regeneration) has shown that the polyacrylic, gel type, weakly basic anion exchanger Purolite A 847 (A 845); polystyrene, macroporous, strongly basic anion exchanger Purolite A 500 PS... [to full text]
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Eurelija, Venskaitytė. "Širdies ir kraujagyslių sistemos funkcinių rodiklių sąsajų kaita vertinant sportuojančiųjų organizmo būsenas." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2011. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20110705_140525-62609.
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Dinaminiai žmogaus organizmo kaip kompleksinės sistemos procesai atsispindi, registruojant fiziologinių procesų signalus, kuriems būdingos įvairaus laipsnio svyravimai, pavyzdžiui – elektrokardiogramos (EKG) signalai. Paprastai šių signalų analizei pasirenkami statistinės analizės metodai, kurie labiau taikytini globaliems organizmo procesams ir kuriems reikalingas didelis kiekis informacijos. Atitinkamai matematinės analizės metodai taikomi lokaliems organizmo procesams nagrinėti ir tuo pačiu nereikalingas toks didelis kiekis informacijos (mūsų atveju pakanka trijų atskaitymų). Fiziologinių signalų matricinė analizė padeda atskleisti sudėtingus žmogaus organizmo kaip KAS bei nuovargio fenomenus, parodančius netiesinių organizmo funkcinės būklės ir adaptacijos procesų fraktalinio ir chaotinio pobūdžio ypatumus. Atsižvelgiant į tai, šio tyrimo tikslas buvo nustatyti širdies ir kraujagyslių sistemos funkcinių rodiklių sąsajų kaitos ypatumus, vertinant sportuojančių asmenų organizmo būsenas.The dynamic of the human body as a complex system of processes is reflected in the registration process of physiological signals which are characterized by varying degrees of oscillations, for example - an electrocardiogram (ECG) signals. Typically, these signals are selected for the statistical analysis methods that are more applicable to global processes of the body and requiring large amounts of information. Accordingly, the mathematical analysis methods are used to examine the local processes of the body and thus do not require such a large amount of information (in our case, enough of the three deductions). Physiological signal analysis matrix helps reveal the complex human organism as complex adaptive system and fatigue phenomena, which reflect the functional state of homeostasis and adaptation processes in fractal and chaotic nature of features. In this context, the aim of the study is to reveal the dynamical peculiarities of interactions of cardiovascular system indices in evaluation of sportsmen body’s functional state.
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Pranaitis, Mindaugas. "Study of carrier transport, trapping and optical nonlinearities in polymers promising for optoelectronic applications." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2012. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2012~D_20121001_093403-67813.
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The main goals of the thesis are advanced characterization by complementary optical and electrical methods of organic semiconductors and the complexes of DNA (deoxyribonucleic acid) designed with purposefully controllable properties for opto-, photo- and electrical applications in modern device engineering. It was demonstrated that the polymer photovoltaic device having an active layer with donor/transmitter/acceptor structure bearing polar molecules exhibits the improvement of the external quantum efficiency associated with the growth of the mobility and reduced potential barrier for charge injection or extraction from the electrodes. The impact of the trapping states and their energetical and spatial distribution on the charge transport properties of organic semiconductors was revealed by several different complementary methods. It was demonstrated that carrier trapping is effectively involved in the charge transport phenomena, depending on the exciting light spectral range and applied electrical field. The influence of the hybrid DNA, dyes and silica material complexes on the optical properties of bio-organic materials was proven. Later the new cationic surfactant with a high efficiency third order nonlinear optical properties was demonstrated, which extended the range of available solvents for DNA complex.Disertacija skirta išanalizuoti veiksnius, įtakojančius krūvio pernašą ir pagavimą šiuolaikinėse organinėse medžiagose skirtose optoelektronikai, bei ištirti DNR bio-molekulių įtaką elektrinėms bei optinėms medžiagų savybėms. Eksperimentiškai nustatyta, kad polimerinės medžiagos turinčios aktyviojo sluoksnio struktūrą: donoras/pernašos grandis/akceptorius ir chemiškai sujungtos su polinėmis molekulėmis, pasižymi išorinio kvantinio našumo padidėjimu, susijusiu su judrio išaugimu ir sumažėjusiu potencialo barjeru krūvininkų injekcijai ar ekstrakcijai iš elektrodų. Panaudojant keletą skirtingų, bet papildančių eksperimentinių metodų, parodyta pagavimo būsenų ir jų energetinio bei erdvinio pasiskirstymo įtaka krūvininkų pernašos savybėms organiniuose puslaidininkiuose. Pademonstruota, kad krūvininkų pagavimas efektyviai lemia krūvio pernašos reiškinius ir priklauso nuo žadinančios šviesos spektro pločio bei pridėto elektrinio lauko. Nustatyta DNR komplekso, sudaryto iš dažo ir silikagelio matricos, įtaka optinėms medžiagos savybėms. Taip pat pademonstruoti nauji katijoniniai surfaktantai su efektyviomis trečios eilės netiesinės optikos savybėmis, kurie išplečia tirpiklių pasirinkimą gaminant DNR kompleksus.
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Maksimavičius, Ričardas. "Ro-ro vežimų tyrimai Baltijos jūroje." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2004. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2004~D_20041112_121030-83290.
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Currently Port of Klaipėda has been interlinked by ro-ro transit lines with Germany, Sweden and Denmark. It has been sought not only for improvement but also for development and expansion of the ro-ro market in order to answer continuously growing needs of clients.
Lithuanian ro-ro liner shippers and operators although confronted by severe competition in ro-ro shipping market, strive to retain their position, conquer and manage new ro-ro markets. Yet it is to be mentioned that close cooperation with other transport industries and ro-ro terminals is not always attainable what consequently enhance the impact of factors affecting ro-ro transport and lessen attractiveness of such transportation, reduce cargo flow in general.
Relevance of the problem. Considerable part of ro-ro lines are forced to close down soon after they are opened as having no prospects for they fail to attract sufficient freight and cargo flow due to multiple reasons like inadequate assessment and underestimation of advantages, shortcomings and risks (threats) and overestimated possibilities, insufficient research into cargo flow, inconsideration or failed acknowledgement of factors affecting ro-ro transportation. Therefore the relevance of the issue involved in this dissertation reveals not only for developing of new ro-ro shipping lines, but also for restructuring of existing lines and restoring of former ro-ro lines, it applies also in view of intended increase in competitiveness of ro-ro freightage... [to full text]
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Svetikas, Mantas. "KIŠKONIŲ KIAULIŲ KOMPLEKSO PROBLEMOS IR JŲ SPRENDIMAI." Bachelor's thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20100907_095043-18062.
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Visą laiką kiaulininkystė Lietuvoje buvo antroji gyvulininkystės šaka, turinti senas ūkininkavimo tradicijas [40]. Kiaulės lyginant su kitomis gyvulių rūšimis, auginti patogu dėl gana trumpo kiaulių auginimo laikotarpio, per kurį bandą galima greitai atkurti arba didinti. Kiaulienos gamybos apimtys pasaulyje pastebimai didesnės už kitų gyvūnų rūšių mėsos gamybą. Pagal ES žemės ūkio produktų rinkos vystimosi prognozes 2006-2013 m. tikimasi, jog bendras mėsos suvartojimas Europos Sąjungoje per artimiausius 3 metus padidės iki 89 kg/gyventojui, o didžiąją jo dalį sudarys kiauliena – apie 44,4 kg/gyventojui. Visame pasaulyje prognozuojamas spartus mėsos poreikio didėjimas, kuriam patenkinti numatoma 20 procentų pasaulinės mėsos gamybos iki 2020 metų augimas. Kiaulininkystė bus svarbi šaka tenkinant šį poreikį. Iš kitos pusės, visuomenė atkreipia dėmesį į kiaulininkystės plėtros pasekmes aplinkai, ypač dėl kiaulių mėšlo įtakos vandeniui ir orui. Keliamos problemos dėl žmonių sveikatos, ypač tose teritorijose, kuriose plėtojamas šios šakos verslas ar žmonės gyvena netoli stambių kiaulininkystės objektų.“Saerimner” Ltd. is Danish capital based company maintainings its activities in Lithuania starting with the year 1999. The company maintains and controls three swine farm complexes, namely Šalnaičių (Pasvalys region), Rupinskų (Ignalina region) and Mūšos (Pakruojis region). “Saerimner” Ltd. main activities involve the growing and feeding of pigs centered in “Sajas“ Ltd. company branch. “Sajas“ Ltd. is a joint venture branch of “Saerimner” Ltd. In this research paper the problems related to the swine farm complexes were analysed and certain measures or suggestions to cope with them have been provided.
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Žukaitis, Vytautas. "Kaimo turizmo paslaugų komplekso formavimas Varėnos rajone." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2006. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2006~D_20060516_094404-78429.
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SUMMARY
MODELLING A COUNTRY TOURISM COMPLEX IN VARENA DISTRICT
Key words: turism, country turism, complex of country tourism services
Problem. Tourism is one of the most rapidly developing business sectors in the world, influencing social and economical wealth of individual regions and fostering the growth of various business sectors (food supply, accomodation, transport, etc.). In Lithuania, the tourism sector can be characterised in growing stage and it has favourable conditions for developing. One of the most promising sectors of Lithuanian tourism is country tourism, defined as a type of tourism containing all tourism services related with getting familiar with country environment and resting in it. The development of country tourism is characterised in state level, where the goal is not only to extend the tourism sector, but also to increase the employment of country people and to accelerate the economical development of country. Science literature which studies the specifics of country tourism is analyzed in this work. The empirical research based on the analysis mentioned allows to define the main spheres that require more attention from country tourism service providers in order to ensure the tourist satisfaction. The research is conducted while analysing the attitudes of country tourism owners and their clients in Varena Disctrict towards the complex of country tourism services.
The object of research – country tourism services in Varena District.
The objective... [to full text]
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Montvidienė, Kristina. "Traukos riedmenų įsigijimo terminų AB „Lietuvos geležinkeliai“ prognozavimas." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2005. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2005~D_20050616_150451-44570.
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In this work are present analysis of traction rolling – stock in Lithuania. There are traversed main criterias of 2M62 series lokomotyve of Radviliškis lokomotyve depot – expenses of maintenance and outlays of fuel, oil, unplanned repairs, running repairs ER-1 and technical handling TP-3.According to complex quality rate is determined critical technical state of traction rolling-stock. There is designed expedient technique of selection of traction rolling-stock and terms of purchase, invoking which we could get maximum economical effect.Inferences are present in the end of this work.
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Petrauskas, Karolis. "Kompiuterinis sudėtinės geometrijos biojutiklių modeliavimas." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2011. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20110701_105902-81823.
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Biojutikliai yra įrenginiai, skirti medžiagoms aptikti bei jų koncentracijoms matuoti. Siekiant sumažinti biojutiklių gamybos kaštus yra pasitelkiamas matematinis biojutikliuose vykstančių procesų modeliavimas. Disertacijoje nagrinėjami matematiniai ir kompiuteriniai biojutiklių modeliai, aprašantys biojutiklių, sudarytų iš kelių, skirtingas savybes turinčių dalių, veikimą. Nagrinėjami modeliai yra formuluojami vienmatėje bei dvimatėje erdvėse, aprašomi diferencialinėmis lygtimis dalinėmis išvestinėmis su netiesiniais nariais ir yra sprendžiami skaitiškai, naudojant baigtinių skirtumų metodą. Skaitiniai modeliai yra įgyvendinami kompiuterine programa. Disertacijoje pateikiamas originalus matematinis modelis biojutikliui su anglies nanovamzdelių elektrodu, nustatyti kriterijai, apibrėžiantys, kada biojutiklį su perforuota membrana galima modeliuoti vienmačiu modeliu. Darbe susisteminti elementai, naudojami biojutiklių modelių formulavimui, pagrindinį dėmesį skiriant biojutiklio struktūrinėms savybėms modeliuoti. Apibrėžta biojutiklių modelių aprašo kalba ir sukurta programinė įranga, leidžianti modeliuoti biojutiklių veikimą vienmačiais modeliais arba modeliais, formuluojamais stačiakampėje dvimatės erdvės srityje. Taikant sukurtą biojutiklių modeliavimo programinę įrangą, ištirtas biojutiklio su anglies nanovamzdelių elektrodu modelio adekvatumas ir struktūrinių bei geometrinių savybių įtaka biojutiklio elgsenai.Biosensors are analytical devices mainly used to detect analytes and measure their concentrations. Mathematical modeling is widely used for optimizing and analyzing an operation of biosensors for reducing price of development of new biosensors. The object of this research is mathematical and computer models, describing an operation of biosensors, made of several parts with different properties. The dissertation covers models, formulated in one and two-dimensional spaces by partial differential equations with non-linear members, and solved numerically, using the method of finite differences. The numerical models are implemented by a computer program. An original mathematical model for a biosensor with a carbon nanotube electrode is presented in the dissertation. The conditions at which the one-dimensional mathematical model can be used instead of two-dimensional one for accurate prediction of the biosensor response are investigated. Elements, used to build models of biosensors with a complex structure, were systemized. The biosensor description language is proposed and the computer software, simulating an operation of biosensors in the one-dimensional space and a rectangular domain of the two-dimensional space, is developed. An adequateness of the model for the biosensor with the carbon nanotube electrode and the impact of structural and geometrical properties on a response of the biosensor were investigated, performing computer experiments using the developed software.
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Naujokas, Tomas. "Duomenų prieinamumo ir saugumo duomenų bazėse metodiniai nurodymai." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2013. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2012~D_20131105_103132-65944.
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Šio darbo tikslas buvo sukurti reikalavimais grindžiamą duomenų saugumo ir matomumo metodų pasirinkimo bei jų kombinavimo metodinę medžiagą. Pateikti reikalavimas grindžiamą kompleksinės saugos modelį. Pašalinti kompleksinės apsaugos metodų informacijos trūkumą. Darbe išnagrinėti ir palyginti garsių pasaulio saugos specialistų kompleksinės saugos sprendimai. Atlikta sistemos pažeidimų analizė ir sisteminimas. Darbe buvo siekiama atskleisti svarbiausius pažeidimus, jų veikimo principus ir kaip nuo jų tinkamai apsisaugoti. Praktinėje dalyje aprašytas kompleksinis saugos modelis, kuris vėliau smulkinamas į detalius apsaugos modelius. Modelis buvo pritaikytas šiandien populiariausiose kombinuotoje sistemose Microsoft Windows Server 2008 serveryje ir Microsoft SQL Server 2008 duomenų bazių valdymo sistemoje. Naudojantis metodika galima atlikti esamos sistemos saugumo analizę ir remiantis veiklos modeliais teisingai konfigūruoti esamą ar naujai kuriamą sistemą.This work destination was to create requirements based on data security and availability method choosing and their combination methodology. Introduce requirements based on complex security model. Eliminate information lack of complex security. In work analyzed and compared complex security solutions of famous the word security specialists. Accomplished system vulnerability analysis and systematized information. During the work revealed most important vulnerabilities, explain how it works and how correctly secure of them. In this research described security model of complex security, which later detailed as smaller part of model. Complex security model were used at nowadays most popular combined information system. For research were used Microsoft Windows Server 2008 and Microsoft SQL Server 2008. Created methodology is useful then necessary to test existing or creating new configuration of system.