Relevant bibliographies by topics / LOPAC

Academic literature on the topic 'LOPAC'

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Published: 6 September 2023

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Journal articles on the topic "LOPAC"

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McCallum, Megan M., Premchendar Nandhikonda, Jonathan J. Temmer, Charles Eyermann, Anton Simeonov, Ajit Jadhav, Adam Yasgar, David Maloney, and Alexander (Leggy) Arnold. "High-Throughput Identification of Promiscuous Inhibitors from Screening Libraries with the Use of a Thiol-Containing Fluorescent Probe." Journal of Biomolecular Screening 18, no. 6 (February 27, 2013): 705–13. http://dx.doi.org/10.1177/1087057113476090.

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Testing small molecules for their ability to modify cysteine residues of proteins in the early stages of drug discovery is expected to accelerate our ability to develop more selective drugs with lesser side effects. In addition, this approach also enables the rapid evaluation of the mode of binding of new drug candidates with respect to thiol reactivity and metabolism by glutathione. Herein, we describe the development of a fluorescence-based high-throughput assay that allows the identification of thiol-reactive compounds. A thiol-containing fluorescent probe, MSTI, was synthesized and used to evaluate small molecules from the Library of Pharmacologically Active Compounds (LOPAC) collection of bioactive molecules. LOPAC compounds that are known to react with sulfur nucleophiles were identified with this assay, for example, irreversible protease inhibitors, nitric oxide–releasing compounds, and proton-pump inhibitors. The results confirm that both electrophilic and redox reactive compounds can be quickly identified in a high-throughput manner, enabling the assessment of screening libraries with respect to thiol-reactive compounds.
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Haus, Patricia, Michael Korbus, Michael Schröder, and Franz-Josef Meyer-Almes. "Identification of Selective Class II Histone Deacetylase Inhibitors Using a Novel Dual-Parameter Binding Assay Based on Fluorescence Anisotropy and Lifetime." Journal of Biomolecular Screening 16, no. 10 (October 25, 2011): 1206–16. http://dx.doi.org/10.1177/1087057111424605.

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Histone deacetylases (HDACs) are important epigenetic factors regulating a variety of vital cellular functions such as cell cycle progression, differentiation, cell migration, and apoptosis. Consequently, HDACs have emerged as promising targets for cancer therapy. The drugability of HDACs has been shown by the discovery of several structural classes of inhibitors (HDACis), particularly by the recent approval of two HDACis, vorinostat (ZOLINZA) and romidepsin (Istodax), for the treatment of cutaneous T-cell lymphoma by the US Food and Drug Administration. The outstanding potential of HDACis, with a defined isoform selectivity profile as drugs against a plurality of diseases, vindicates increased effort in developing high-throughput capable assays for screening campaigns. In this study, a dual-competition assay exploiting changes in fluorescence anisotropy and lifetime was used to screen the LOPAC (Sigma-Aldrich, St Louis, MO) library against the bacterial histone deacetylase homologue HDAH from Bordetella, which shares 35% identity with the second deacetylase domain of HDAC6. The binding assay proved to be highly suitable for high-throughput screening campaigns. Several LOPAC compounds have been identified to inhibit HDAH in the lower micromolar range. Most interestingly, some of the hit compounds turned out to be weak but selective inhibitors of human class IIa and IIb HDACs.
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Chen, Xian, Sudini Ranshaya Fernando, Yin-Lau Lee, William Shu-Biu Yeung, Ernest Hung-Yu Ng, Raymond Hang-Wun Li, and Kai-Fai Lee. "High-Throughput In Vitro Screening Identified Nemadipine as a Novel Suppressor of Embryo Implantation." International Journal of Molecular Sciences 23, no. 9 (May 3, 2022): 5073. http://dx.doi.org/10.3390/ijms23095073.

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Current contraceptive methods interfere with folliculogenesis, fertilization, and embryo implantation by physical or hormonal approaches. Although hormonal contraceptive pills are effective in regulating egg formation, they are less effective in preventing embryo implantation. To explore the use of non-hormonal compounds that suppress embryo implantation, we established a high-throughput spheroid-endometrial epithelial cell co-culture assay to screen the Library of Pharmacologically Active Compounds (LOPAC) for compounds that affect trophoblastic spheroid (blastocyst surrogate) attachment onto endometrial epithelial Ishikawa cells. We identified 174 out of 1280 LOPAC that significantly suppressed BeWo spheroid attachment onto endometrial Ishikawa cells. Among the top 20 compounds, we found the one with the lowest cytotoxicity in Ishikawa cells, P11B5, which was later identified as Nemadipine-A. Nemadipine-A at 10 µM also suppressed BeWo spheroid attachment onto endometrial epithelial RL95-2 cells and primary human endometrial epithelial cells (hEECs) isolated from LH +7/8-day endometrial biopsies. Mice at 1.5 days post coitum (dpc) treated with a transcervical injection of 100 µg/kg Nemadipine-A or 500 µg/kg PRI-724 (control, Wnt-inhibitor), but not 10 µg/kg Nemadipine-A, suppressed embryo implantation compared with controls. The transcript expressions of endometrial receptivity markers, integrin αV (ITGAV) and mucin 1 (MUC1), but not β-catenin (CTNNB1), were significantly decreased at 2.5 dpc in the uterus of treated mice compared with controls. The reduction of embryo implantation by Nemadipine-A was likely mediated through suppressing endometrial receptivity molecules ITGAV and MUC1. Nemadipine-A is a potential novel non-hormonal compound for contraception.
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Turek-Etienne, Tammy C., Eliza C. Small, Sharon C. Soh, Tianpei A. Xin, Priti V. Gaitonde, Ellen B. Barrabee, Richard F. Hart, and Robert W. Bryant. "Evaluation of Fluorescent Compound Interference in 4 Fluorescence Polarization Assays: 2 Kinases, 1 Protease, and 1 Phosphatase." Journal of Biomolecular Screening 8, no. 2 (April 2003): 176–84. http://dx.doi.org/10.1177/1087057103252304.

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With the increasing use of fluorescence-based assays in high-throughput screening (HTS), the possibility of interference by fluorescent compounds needs to be considered. To investigate compound interference, a well-defined sample set of biologically active compounds, LOPAC™, was evaluated using 4 fluorescein-based fluorescence polarization (FP) assays. Two kinase assays, a protease assay, and a phosphatase assay were studied. Fluorescent compound interference and light scattering were observed in both mixture- and single-compound testing under certain circumstances. In the kinase assays, which used low levels (1-3 nM) of fluorophore, an increase in total fluorescence, an abnormal decrease in mP readings, and negative inhibition values were attributed to compound fluorescence. Light scattering was observed by an increase in total fluorescence and minimal reduction in mP, leading to false positives. The protease and phosphatase assays, which used a higher concentration of fluorophore (20-1200 nM) than the kinase assays, showed minimal interference from fluorescent compounds, demonstrating that an increase in the concentration of the fluorophore minimized potential fluorescent compound interference. The data also suggests that mixtures containing fluorescent compounds can result in either false negatives that can mask a potential “hit” or false positives, depending on the assay format. Cy™ dyes (e.g., Cy3B™ and Cy5™) excite and emit further into the red region than fluorescein and, when used in place of fluorescein in kinase 1, eliminate fluorescence interference and light scattering by LOPAC™ compounds. This work demonstrates that fluorescent compound and light scattering interferences can be overcome by increasing the fluorophore concentration in an assay or by using longer wavelength dyes. ( Journal of Biomolecular Screening 2003:176-184)
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Naghaei, R., and M. J. Monem. "Development of a Mathematical Model of Lopac Gates in Accordance with the ICSS Hydrodynamic Model." Journal of Irrigation and Drainage Engineering 142, no. 10 (October 2016): 04016043. http://dx.doi.org/10.1061/(asce)ir.1943-4774.0001066.

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Rabjohns, Jennifer L. A., Yoon-Dong Park, Jean Dehdashti, Wei Sun, Christina Henderson, Adrian Zelazny, Steven J. Metallo, Wei Zheng, and Peter R. Williamson. "A High-Throughput Screening Assay for Fungicidal Compounds against Cryptococcus neoformans." Journal of Biomolecular Screening 19, no. 2 (July 29, 2013): 270–77. http://dx.doi.org/10.1177/1087057113496847.

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Cryptococcus neoformans is a pathogenic fungus that causes meningitis worldwide, particularly in human immunodeficiency virus (HIV)–infected individuals. Although amphotericin B is the “gold standard” treatment for cryptococcal meningitis, the toxicity and inconvenience of intravenous injection emphasize a need for development of new anticryptocccal drugs. Recent data from humans and animal studies suggested that a nutrient-deprived host environment may exist in cryptococcal meningitis. Thus, a screening assay for identifying fungicidal compounds under nutrient-deprived conditions may provide an alternative strategy to develop new anticryptococcal drugs for this disease. A high-throughput fungicidal assay was developed using a profluorescent dye, alamarBlue, to detect residual metabolic activity of C. neoformans under nutrient-limiting conditions. Screening the Library of Pharmacologically Active Compounds (LOPAC) with this assay identified a potential chemical scaffold, 10058-F4, that exhibited fungicidal activity in the low micromolar range. These results thus demonstrate the feasibility of this alamarBlue-based assay for high-throughput screening of fungicidal compounds under nutrient-limiting conditions for new anticryptococcal drug development.
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Caporale, Andrea, Fabiola Mascanzoni, Biancamaria Farina, Mattia Sturlese, Gianluigi Di Sorbo, Roberto Fattorusso, Menotti Ruvo, and Nunzianna Doti. "FRET-Protease-Coupled Peptidyl-Prolyl cis-trans Isomerase Assay." Journal of Biomolecular Screening 21, no. 7 (July 10, 2016): 701–12. http://dx.doi.org/10.1177/1087057116650402.

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In this work, a sensitive and convenient protease-based fluorimetric high-throughput screening (HTS) assay for determining peptidyl-prolyl cis-trans isomerase activity was developed. The assay was based on a new intramolecularly quenched substrate, whose fluorescence and structural properties were examined together with kinetic constants and the effects of solvents on its isomerization process. Pilot screens performed using the Library of Pharmacologically Active Compounds (LOPAC) and cyclophilin A (CypA), as isomerase model enzyme, indicated that the assay was robust for HTS, and that comparable results were obtained with a CypA inhibitor tested both manually and automatically. Moreover, a new compound that inhibits CypA activity with an IC50 in the low micromolar range was identified. Molecular docking studies revealed that the molecule shows a notable shape complementarity with the catalytic pocket confirming the experimental observations. Due to its simplicity and precision in the determination of extent of inhibition and reaction rates required for kinetic analysis, this assay offers many advantages over other commonly used assays.
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Ajit, Seena K., Mark H. Pausch, Jeffrey D. Kennedy, and Edward J. Kaftan. "Development of a FLIPR Assay for the Simultaneous Identification of MrgD Agonists and Antagonists from a Single Screen." Journal of Biomedicine and Biotechnology 2010 (2010): 1–8. http://dx.doi.org/10.1155/2010/326020.

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MrgD, a member of the Mas-related gene family, is expressed exclusively in small diameter neurons in the dorsal root ganglion. This unique expression pattern, the presence of a single copy of MrgD in rodents and humans, and the identification of a putative ligand, beta-alanine, make it an experimentally attractive therapeutic target for pain with limited likelihood of side effects. We have devised a high throughput calcium mobilization assay that enables identification of both agonists and antagonists from a single screen for MrgD. Screening of the Library of Pharmacologically Active Compounds (LOPAC) validated this assay approach, and we identified both agonists and antagonists active at micromolar concentrations in MrgD expressing but not in parental CHO-DUKX cell line. Further characterization was performed using a subset of these screening hits. Our results demonstrated that the dual agonist/antagonist assay format is feasible and likely can be extended to most GPCRs with known agonist.
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Camara, Ali, Alyssa George, Evan Hebner, Anika Mahmood, Jashun Paluru, and Seema Mattoo. "A Fluorescence Polarization-Based High-Throughput Screen to Identify the First Small-Molecule Modulators of the Human Adenylyltransferase HYPE/FICD." International Journal of Molecular Sciences 21, no. 19 (September 27, 2020): 7128. http://dx.doi.org/10.3390/ijms21197128.

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The covalent transfer of the AMP portion of ATP onto a target protein—termed adenylylation or AMPylation—by the human Fic protein HYPE/FICD has recently garnered attention as a key regulatory mechanism in endoplasmic reticulum homeostasis, neurodegeneration, and neurogenesis. As a central player in such critical cellular events, high-throughput screening (HTS) efforts targeting HYPE-mediated AMPylation warrant investigation. Herein, we present a dual HTS assay for the simultaneous identification of small-molecule activators and inhibitors of HYPE AMPylation. Employing the fluorescence polarization of an ATP analog fluorophore—Fl-ATP—we developed and optimized an efficient, robust assay that monitors HYPE autoAMPylation and is amenable to automated, high-throughput processing of diverse chemical libraries. Challenging our pilot screen with compounds from the LOPAC, Spectrum, MEGx, and NATx libraries yielded 0.3% and 1% hit rates for HYPE activators and inhibitors, respectively. Further, these hits were assessed for dose-dependency and validated via orthogonal biochemical AMPylation assays. We thus present a high-quality HTS assay suitable for tracking HYPE’s enzymatic activity, and the resultant first small-molecule manipulators of HYPE-promoted autoAMPylation.
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Weizhen Wu, Jin Shang, Yue Feng, Chris M. Thompson, Sarah Horwitz, John R. Thompson, Euan D. Macintyre, et al. "Identification of Glucose-Dependent Insulin Secretion Targets in Pancreatic β Cells by Combining Defined-Mechanism Compound Library Screening and siRNA Gene Silencing." Journal of Biomolecular Screening 13, no. 2 (January 23, 2008): 128–34. http://dx.doi.org/10.1177/1087057107313763.

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Identification and validation of novel drug targets continues to be a major bottleneck in drug development, particularly for polygenic complex diseases such as type 2 diabetes. Here, the authors describe an approach that allows researchers to rapidly identify and validate potential drug targets by combining chemical tools and RNA interference technology. As a proof-of-concept study, the known mechanism Sigma LOPAC library was used to screen for glucose-dependent insulin secretion (GDIS) in INS-1 832/13 cells. In addition to several mechanisms that are known to regulate GDIS (such as cyclic adenosine monophosphate—specific phosphodiesterases, adrenoceptors, and Ca2+ channels), the authors find that several of the dopamine receptor ( DRD) antagonists significantly enhance GDIS, whereas DRD agonists profoundly inhibit GDIS. Subsequent siRNA studies in the same cell line indicate that knockdown of DRD2 enhanced GDIS. Furthermore, selective DRD2 antagonists and agonists also enhance or suppress, respectively, GDIS in isolated rat islets. The data support that the approach described here offers a rapid and effective way for target identification and validation. ( Journal of Biomolecular Screening 2008;128-134)
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Dissertations / Theses on the topic "LOPAC"

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Hrňa, Lukáš. "Úprava rychloupínacího zařízení pro aplikace prosévacích lopat." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2013. http://www.nusl.cz/ntk/nusl-230539.

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This thesis deals with the adjustment of quit camping equipment for application on screener crusher bucket Allu SC 3-20 in order to streamline both digging into the bucket, and the actual screening. Using simulation methods in the Adams programme, forces are detected for the digging with the original suspension. Imperfections are revealed for the current full suspension and suggestions made for the new proposedsuspension. The new shape quick release mechanism is designed in CATIA modeler and the I-Deas programme provides final strength control of parts for the proposed mechanism. Finally, both variants are coMPared in terms of digging, sifting the material complexity of the design and the necessary restrictive criteria.
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Frank, Matthew I. "LoPC-- modeling contention in parallel algorithms." Thesis, Massachusetts Institute of Technology, 1997. http://hdl.handle.net/1721.1/47439.

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Kutzer, Pia Elfriede Seyra [Verfasser], and Kai [Gutachter] Lopau. "Langzeitergebnisse einer steroidfreien Immunsuppression ein Jahr nach Nierentransplantation / Pia Elfriede Seyra Kutzer ; Gutachter: Kai Lopau." Würzburg : Universität Würzburg, 2016. http://d-nb.info/1122021011/34.

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Silva, Edlaine Correia Sinézio da Silva. "Desenvolvimento da metodologia Lopa-Bayesiana em dois estágios / Edlaine Correia Sinézio da Silva." Universidade Federal de Pernambuco, 2013. https://repositorio.ufpe.br/handle/123456789/11845.

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Submitted by Irene Nascimento (irene.kessia@ufpe.br) on 2015-03-10T19:43:19Z No. of bitstreams: 2 DISSERTAÇÃO Edlaine Correia Sinézio da Silva.pdf: 4116734 bytes, checksum: 217d9a6dc7fa0ef298e86b4692361334 (MD5) license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5)
Made available in DSpace on 2015-03-10T19:43:19Z (GMT). No. of bitstreams: 2 DISSERTAÇÃO Edlaine Correia Sinézio da Silva.pdf: 4116734 bytes, checksum: 217d9a6dc7fa0ef298e86b4692361334 (MD5) license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Previous issue date: 2013-05-23
Capes
Nas últimas décadas o Gás Natural Liquefeito- GNL tem se destacado enquanto promissora fonte de energia e consequentemente sua utilização vem crescendo consideravelmente. Todavia, devido à natureza inflamável do metano- principal componente do GNL- a ocorrência de acidentes com o seu vazamento nos terminais de transporte e armazenamento podem proporcionar perigo para a sociedade. Dentre os principais perigos associados ao GNL, está transição rápida de fase (RTP), incêndio em poça, incêndio em nuvem e explosões. Neste contexto, a Análise de Camadas de Proteção (LOPA) é uma forma simplificada de avaliação de risco que fornece resultados quantificados de risco com menos tempo e esforço do que a Análise Quantitativa de Riscos (AQR), por exemplo. A LOPA é um método semi-quantitativo que gera uma estimativa numérica da frequência de falha do cenário mitigado. Para o cálculo da frequência de falha do cenário, é necessário obter dados de falha. Contudo, por tratar-se de um terminal de GNL, os dados de falhas de equipamentos são esparsos, não sendo estatisticamente confiáveis por tratar-se de uma indústria recente. Neste caso, a análise Bayesiana é uma ótima ferramenta, pois possibilita utilizar dados específicos da planta em estudo e dados genéricos. Sejam os dados genéricos obtidos nos bancos de dados procedentes de várias indústrias, operando em diferentes condições, faz-se necessário considerar a não-homogeneidade da população. No entanto, na literatura encontra-se aplicações clássica da análise Bayesiana. Sendo assim, esta pesquisa propôs melhorar a metodologia apresentada na literatura utilizando os mesmos dados, porém empregando a Análise Bayesiana em Dois Estágios. O primeiro estágio é uma análise não homogênea, que considera a variabilidade populacional dos dados de falha entre os bancos de dados, e o segundo estágio gera uma distribuição a posteriori atualizada após a introdução dos dados específicos da planta. Finalmente, esta pesquisa comprovou que a metodologia LOPA-Bayesiana em Dois Estágios é mais viável, pois ela apresentou para a frequênca dos cenários mitigados, valores superiores aos encontrados em pesquisa anterior, o que confirma a subestimação do nível de incerteza.
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Steigenberger, Jana Su [Verfasser], Kai [Gutachter] Lopau, and Ingo [Gutachter] Klein. "Kosten der Nierentransplantation in Abhängigkeit von der Transplantatfunktion / Jana Su Steigenberger. Gutachter: Kai Lopau ; Ingo Klein." Würzburg : Universität Würzburg, 2015. http://d-nb.info/1111124930/34.

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Muniz, Márcio Vinicios Pereira. "Análise crítica da contribuição da técnica lopa na gestão de segurança de processo na indústria." Universidade Federal Fluminense, 2016. https://appdesenv.uff.br/riuff/handle/1/1935.

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Submitted by Marcia Silva (marcia@latec.uff.br) on 2016-07-19T19:28:45Z No. of bitstreams: 1 Dissertacao_MSG_Marcio_Muniz_Final.pdf: 2620890 bytes, checksum: b3ca9261af59621b5f9407cc5ee2bb4c (MD5)
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O objetivo da presente dissertação é desenvolver uma análise crítica da contribuição da metodologia LOPA para a gestão de segurança de processo na indústria e perpetuar sua aplicação. Como modelo metodológico, utilizou-se a pesquisa bibliográfica e a pesquisa exploratória, através de um questionário aplicado. Utilizaram-se os resultados do questionário para avaliar o perfil dos respondentes, testou-se a confiabilidade interna das escalas do questionário através da utilização do alfa de Cronbach e executou-se um modelo de triangulação a luz do referencial teórico, dos resultados do questionário e da experiência e dos conhecimentos teóricos do pesquisador. Como resultados, detectou-se a baixa disponibilidade de estudos guiados pela comunidade científica internacional que efetivamente relacionem a utilização da metodologia LOPA com a gestão de segurança de processos, confirmou-se o baixo nível de difusão do conhecimento e de utilização da metodologia LOPA e detectou-se a alta relevância de inclusão da metodologia em um sistema de gestão de segurança de processos. As confiabilidades internas calculadas para os clusters do questionário obtiveram classificação alta e muito alta.
The main goal of this Dissertation is develop a critical analysis of the LOPA methodology contribution to the Process Safety Management in the Industry and to disseminate the LOPA. The Dissertation methodological model included a Bibliographical Research and an Exploratory Research (Through a survey applied, mainly, to the process safety professionals. The Dissertation tested the internal reliability of the survey scales (Through the Cronbach alfa tool), evaluated the survey participants profile and used a triangulation model considering the theoretical framework, the survey results and the experience and theoretical knowledge of the researcher. The Results detected the low availability of studies guided by international scientific community that effectively connect the methodology LOPA with Process Safety Management, the low level of dissemination of knowledge and the low utilization frequency of methodology LOPA and detected the high significance of LOPA inclusion in a Process Safety Management System. The internal reliability values calculated to the survey clusters obtained high and very high classification.
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Au, Thi Huong. "Optimisation et manipulation d'une source de photons uniques par des structures photoniques 2D et 3D à base de matériau polymère à température ambiante." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLN046.

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Cette thèse a été consacrée à l'étude théorique et expérimentale du couplage contrôlé de la source de photon unique (SPS) aux structures photoniques multidimensionnelles, par l'utilisation de la méthode dite écriture directe par laser (DLW) par absorption ultra-faible à un photon (LOPA). La thèse est constitutée de trois parties principales suivantes:La première partie concerne le caractérisation et l'optimisation des dots quantiques (QD) colloïdaux CdSe/CdS. La dépendance de la longeur d'onde d'excitation a été étudiée. En utilisant une excitation à 532 nm, seul le core est excité et l’effet d'Auger est donc supprimé. Cette approche permet donc d'obtenir avec la suppression de la commutation intermittente et une source de photon unique très stable à température ambiante. Afin d’obtenir une meilleure performance des QDs sur une longue période, nous avons ensuite étudié l'influence du milieu dans lequel les QDs sont logés sur leurs propriétés optiques. En intégrant les QDs dans les matrices de polymère SU-8, nous avons montré que l'environnement polymérique permet non seulement de conserver de bonnes caractéristiques des QDs CdSe/CdS avec une photostabilité élevée, mais également de nous offrir une excellente accessibilité à la fabrication des structures en polymère contenant une particule unique.Dans la deuxième partie, la technique LOPA DLW est utilisée pour le couplage des QDs uniques dans diverses structures photoniques. Deux dispositifs, l’antenne du type ''pilier diélectrique'' sous-lambda et la membrane de réseau circulaire (cavité du type ''bulleye''), ont été étudiés théoriquement et expérimentalement pour améliorer l’émission du QD couplé en termes de l'émission spatiale et de l’émission radiative spontanée de l’émetteur.Dans la troisième partie, la manipulation de la SPS est démontrée en couplant le QD unique à des structures magnéto-photoniques multidimensionnelles. À l'aide d'un champ magnétique externe, le mouvement contrôlable d'un seul QD a été démontré dans un environnement fluidique. En contrôlant l'amplitude et l'orientation du champ magnétique externe, la position et l'orientation de la SPS à base d'un QD ont été manipulées à la demande. Les propriétés optiques, magnétiques et mécaniques des dispositifs magnéto-photoniques hybrides ont été étudiées pour montrer les capacités multifonctionnelles de telles structures
The thesis has been devoted to study the controlled coupling of a colloidal quantum dot (QD) based single photon source (SPS) into multidimensional polymeric photonic structures by using low-one photon absorption (LOPA) direct laser writing (DLW) technique. The thesis consists of three main parts:The first part addresses the characteristic optimization of the CdSe/CdS based SPS. The excitation wavelength dependence of the QDs was investigated. By using 532 nm, only the core of the QD is excited with the suppression of the Auger effects. Thus, this approach allows for obtaining the suppression of fluorescence intermittency and a stable single-photon emission at ambient conditions. In order to obtain the long-term high fluorescence quality of the QDs, we then studied the influence of the local dielectric medium on the optical properties of the QDs. By incorporating the QDs into a photoresist (SU-8), we demonstrated that the polymeric environment not only enables the long-term preservation of the QD with high photostability but also provides us excellent accessibility to fabricate polymeric structures containing SPS.In the second part, the LOPA-based DLW is employed for the coupling of single QD into various photonic structures. Two devices including submicropillar dielectric antenna and 3D membrane bulleye cavity are theoretically and experimentally investigated to enhance the fluorescence emission of the single QD in terms of far-field angular radiation pattern and the spontaneous radiative emission of the emitter.In the third part, the manipulation of SPS is demonstrated by coupling the single QD into multidimensional magneto-photonic structures. With the aid of an external magnetic field, the controllable movement of the coupled QD was performed in the fluidic environment. The position and orientation of the SPS coupled in the structure were manipulated on demand. The mechanical, magnetic and optical properties of the device are investigated showing the multifunctional capabilities of magneto-photonic structures
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Kurtz, Stefanie Corinne [Verfasser], Kai [Gutachter] Lopau, and Hubertus [Gutachter] Riedmiller. "Die Anwendung von Donor-Score-Systemen am Beispiel des Nierentransplantationsprogrammes Würzburg / Stefanie Corinne Kurtz. Gutachter: Kai Lopau ; Hubertus Riedmiller." Würzburg : Universität Würzburg, 2015. http://d-nb.info/1111783950/34.

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Fernandes, Sergio. "Produção de proteína LOPAP recombinante (protease ativadora de protrombina da lagarta Lonomia obliqua), purificação, avaliação de estabilidade e estudos estruturais." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-27012015-100207/.

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LOPAP, proteína isolada da toxina de lagartas Lonomia obliqua, possui ação ativadora de protrombina, efeito pró-coagulante e ação citoprotetora em células do endotélio humano, em cultura. Tem cadeia única com 181 resíduos de aminoácidos e 21 kDa. Sua estrutura terciária é formada por oito folhas-b fechadas em uma extremidade, mantidas juntas por pontes de hidrogênio, em formato de barril. Está classificada como pertencente ao grupo das Lipocalinas (proteínas de transporte). Neste trabalho estudou-se o LOPAP, que foi produzido recombinante em cultivo de Pichia pastoris em biorreator e purificado. Avaliou-se sua estabilidade quanto às atividades enzimática e citoprotetora, e sua estrutura secundária. Não foi detectada ativação de protrombina para o r-LOPAP obtido, mas foi observada ação citoprotetora. Considerando estes resultados e a análise de sua estrutura secundária por dicroísmo circular, concluiu-se que a proteína foi expressa com tamanho e sequência corretos, mas sem uma estrutura terciária correta, o que é determinante para a atividade enzimática.
LOPAP, a protein isolated from the toxin of Lonomia obliqua caterpillars, has prothrombin activation action, procoagulant effect and cytoprotection action in human endothelium cells culture. It has only chain with 181 amino acid residues and 21 kDa of size. Its tertiary structure is made by eight b-sheets closed at one end, hold together by hydrogen bonds, barrel-shaped. It is classified as belonging to the Lipocalin group (proteins of transport). This work studied the LOPAP, which was produced recombinant in Pichia pastoris culture in bioreactor, was purified, and it was evaluated its stability related to enzymatic and cytoprotection activities, and its secondary structure. It was not detected prothrombin activation for the r-LOPAP obtained, but it was observed a cytoprotective effect. Regarding these results and the analysis of its secondary structure, by circular dichroism, it was concluded that the protein was expressed with correct size and sequence, but without a correct tertiary structure, which is determinant for the enzymatic activity.
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Wlian, Luana [UNIFESP]. "Ação de um Peptídeo Derivado do Lopap em Cultura de Fibroblastos e em Modelo Experimental de Lesão Cutânea em Ratos." Universidade Federal de São Paulo (UNIFESP), 2009. http://repositorio.unifesp.br/handle/11600/8863.

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Made available in DSpace on 2015-07-22T20:49:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-06-24. Added 1 bitstream(s) on 2015-08-11T03:25:28Z : No. of bitstreams: 1 Publico-00275a.pdf: 1984852 bytes, checksum: b59b9eec047c1dde85258ab2627be073 (MD5). Added 1 bitstream(s) on 2015-08-11T03:25:28Z : No. of bitstreams: 2 Publico-00275a.pdf: 1984852 bytes, checksum: b59b9eec047c1dde85258ab2627be073 (MD5) Publico-00275b.pdf: 1888267 bytes, checksum: ec53e005babae0faeb59e5531e84717a (MD5). Added 1 bitstream(s) on 2015-08-11T03:25:28Z : No. of bitstreams: 3 Publico-00275a.pdf: 1984852 bytes, checksum: b59b9eec047c1dde85258ab2627be073 (MD5) Publico-00275b.pdf: 1888267 bytes, checksum: ec53e005babae0faeb59e5531e84717a (MD5) Publico-00275c.pdf: 1700699 bytes, checksum: 0b64f1d6737aec3687de494ae30df256 (MD5)
O processo de reparação tecidual é um complexo de reações entre células e mediadores bioquímicos que são desencadeados a partir de uma lesão. Neste estudo avaliamos a ação do peptídeo 4 obtido a partir da sequência de aminoácidos do Lopap (Lonomia obliqua prothrombin activator protease) em cultura de fibroblastos para se avaliar a expressão de moléculas de matriz extracelular e receptores de membrana de interleucina-8 e em modelo experimental de lesão cutânea em ratos para se analisar o efeito da molécula em estudo no processo de reparação tecidual. Foram utilizados 40 ratos Wistar machos de 6 a 8 semanas, randomizados em 5 grupos experimentais. Após a anestesia, os ratos foram submetidos a procedimento cirúrgico, onde oito secções circulares de pele de 6 mm de diâmetro foram circunscritas na região dorsal e tratadas com solução salina ou com o peptídeo 4. Após diferentes períodos (3, 7, 14 e 21 dias) realizaram-se análises para se avaliar o processo de reparação tecidual entre os grupos. Concluímos que o peptídeo 4 é capaz de estimular fibroblastos em cultura, além de promover o aceleramento do processo de reparação tecidual em modelo in vivo, com maior deposição de colágeno, glicosaminoglicanos e melhor reorganização tecidual.
The process of tissue repair is a complex of reactions between cells and biochemical mediators that are triggered from an injury. This study evaluated the effect of peptide 4 derived from the amino acid sequence of Lopap (Lonomia obliqua prothrombin activator protease) in culture of fibroblasts to evaluate the expression of molecules of extracellular matrix and the membrane receptors of interleukin-8 and in experimental model of skin lesion in rats to examine the effect of the molecule in the process of tissue repair. We used 40 male Wistar rats with 6 to 8 weeks, randomized to 5 experimental groups. After anesthesia, the rats were submitted to surgery, where eight circular full-thickness of skin from 6 mm of diameter were surrounded in the back and treated with saline or with peptide 4. After different periods (days 3, 7, 14 and 21 days after wounding) were carried out tests to evaluate the process of tissue repair between the groups. We conclude that peptide 4 is able to stimulate fibroblasts in culture and promote the acceleration of the process of tissue repair in vivo model with greater deposition of collagen, glycosaminoglycans and better tissue reorganization.
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Books on the topic "LOPAC"

1

Vuković, Momo. Župski lonac. Nikšić: Slobodna misao, 2006.

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Močvarni Lovac. Zagreb: Znanje, 1995.

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Lovac mirisa. Vinkovci: Privlačica, 1994.

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Menković, Osman. Bosanski lonac. Sanski Most: Kalem, 2021.

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Hosseini, Khaled. Lovac na zmajeve. 3rd ed. Beograd: Laguna, 2008.

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Lovac i kazna. Vršac: Književna opština Vršac, 2007.

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Lovac na zene. Beograd: Apostrof, 2000.

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Dukić, Zlatko. Lovac na štakore: Roman. Sarajevo: BZK Preporod, 2011.

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Lovac na smiren dah. Zagreb: Matica hrvatska, 2004.

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Lovac u labirintu: Eseji & elzeviri. Zagreb: Naklada Ljevak, 2006.

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Book chapters on the topic "LOPAC"

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Gooch, Jan W. "Lopac." In Encyclopedic Dictionary of Polymers, 434. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_7032.

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Lu, Sha, Lin Liu, Jiuyong Li, Thuc Duy Le, and Jixue Liu. "LoPAD: A Local Prediction Approach to Anomaly Detection." In Advances in Knowledge Discovery and Data Mining, 660–73. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-47436-2_50.

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Singh, Ashish, and Kakali Chatterjee. "LoBAC: A Secure Location-Based Access Control Model for E-Healthcare System." In Algorithms for Intelligent Systems, 621–28. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-5243-4_58.

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Villanueva, Jesús David Terán, Héctor Joaquín Fraire Huacuja, Rodolfo Pazos Rangel, Juan Martín Carpio Valadez, Héctor José Puga Soberanes, and Juan Javier González Barbosa. "Iterated Local Search Algorithm for the Linear Ordering Problem with Cumulative Costs (LOPCC)." In Studies in Computational Intelligence, 395–404. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-15534-5_24.

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Overton, Tim, and Tim Wagner. "Use of Layer of Protection Analysis (LOPA) within the Dow Chemical Company." In Emergency Planning Preparedness, Prevention & Response, 347–53. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470924839.ch29.

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Villanueva, David Terán, Héctor Joaquín Fraire Huacuja, Abraham Duarte, Rodolfo Pazos R., Juan Martín Carpio Valadez, and Héctor José Puga Soberanes. "Improving Iterated Local Search Solution for the Linear Ordering Problem with Cumulative Costs (LOPCC)." In Knowledge-Based and Intelligent Information and Engineering Systems, 183–92. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-15390-7_19.

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"Lopac." In Encyclopedic Dictionary of Polymers, 583. New York, NY: Springer New York, 2007. http://dx.doi.org/10.1007/978-0-387-30160-0_6922.

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"Implementing LOPA." In Layer of Protection Analysis, 151–62. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470935590.ch9.

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"Advanced LOPA Topics." In Layer of Protection Analysis, 173–90. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470935590.ch11.

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"Overview of LOPA." In Layer of Protection Analysis, 11–30. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470935590.ch2.

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Conference papers on the topic "LOPAC"

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Das, Viswanath, Tomáš Fürst, Soňa Gurská, Petr Džubák, and Marián Hajdúch. "Abstract 3254: Identification of potent anti-tumor agents from the LOPAC library using a unified 2D/3D cell culture screening approach." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-3254.

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Frank, Matthew I., Anant Agarwal, and Mary K. Vernon. "LoPC." In the sixth ACM SIGPLAN symposium. New York, New York, USA: ACM Press, 1997. http://dx.doi.org/10.1145/263764.263803.

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Garagic, Denis, Jacob Peskoe, Fang Liu, Manuel Cuevas, Andrew M. Freeman, and Bradley J. Rhodes. "Long-range dismount activity classification: LODAC." In SPIE Defense + Security, edited by Michael A. Kolodny. SPIE, 2014. http://dx.doi.org/10.1117/12.2053090.

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Gheyasi, Seyed Mohsen, and Mohammad Pourgol-Mohammad. "Modified Layer of Protection Analysis for Nuclear Safety Assessment." In 2014 22nd International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/icone22-30828.

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Layer of Protection Analysis (LOPA) is extensively used for safety analysis of non-nuclear facilities e.g., chemical processes. It is simpler and less expensive methodology comparing full probabilistic risk assessment methods. It evaluates the probability of failure per demand for the safety system failures and the resulting consequences. LOPA is widely used in chemical process safety analysis however it is introduced as a practical technique for risk assessing in many other industries. This research examines utilization of LOPA method for nuclear systems as a quick evaluation of the safety level. The changes are proposed to classic LOPA method by using event tree method for event scenario identification and development. The so-called modified layer of protection analysis (Modified-LOPA) methodology utilizes Bayesian updating for more precise quantification of the failure probabilities and updating data. A simple example of a fire protection system shows application of this method.
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King, A. G. "SIL determination and the application of LOPA." In 4th IET Seminar on SIL Determination: Minimising the Risk of your Systems. IEE, 2008. http://dx.doi.org/10.1049/ic:20080659.

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Chambers, C. "SIL determination: pitfalls found in LOPA studies." In 4th IET Seminar on SIL Determination: Minimising the Risk of your Systems. IEE, 2008. http://dx.doi.org/10.1049/ic:20080661.

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Jiang, Ying, Zhi-Guo Yang, and Lian-Gang Lu. "The localization of marine mammal with LoPAS-D system." In 2016 IEEE/OES China Ocean Acoustics (COA). IEEE, 2016. http://dx.doi.org/10.1109/coa.2016.7535773.

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Lokhande, Smita, Vishnu P. Menon, and Yogesh Kumar Bichpuriya. "Modelling of demand response for utility's loac forecasting." In 2017 14th International Conference on the European Energy Market (EEM). IEEE, 2017. http://dx.doi.org/10.1109/eem.2017.7981985.

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Scott-Murrell, Emrys, David Lanza, and Michael J. Schertzer. "Impedimetric Feedback in Particle Laden Digital Microfluidic Devices." In ASME 2015 13th International Conference on Nanochannels, Microchannels, and Minichannels collocated with the ASME 2015 International Technical Conference and Exhibition on Packaging and Integration of Electronic and Photonic Microsystems. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/icnmm2015-48494.

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Impedimetric measurement methods are a novel approach to the characterization of fluid in biological applications. Lab on a chip (LOAC) technologies could be combined with impedimetrics to benefit these applications. LOAC devices are currently being developed to pursue the miniaturization of larger scale processes. Current research shows great flexibility in using LOAC devices to reproduce biological processes such as those used in medical diagnostic applications. With a smaller form factor, testing that generally requires off-site lab usage can be deployed at the point-of-care. LOAC devices also have the potential to lower operating costs by reducing reagent volumes, labor costs, and cycle times. Digital microfluidic devices (DMF) are one promising LOAC platform. These devices manipulate discrete droplets of fluid using electric fields. As such, DMF devices can create, move, merge, and mix droplets while eliminating mechanical components like channels, pumps, and valves. Manipulation of discrete volumes over a planar array of electrodes allows for the possibility of highly flexible, reconfigurable devices. Addressable positions on a DMF device have conductive planes above and below the droplets which form a parallel plate capacitor. Using this principle, the electrical properties of the system can be measured in the same circuit that is used for droplet manipulation, removing the need for additional sensing components. This research tests the hypothesis that the impedance of a particle laden droplet in a DMF device can be modelled using an equivalent circuit model for particles that span more than half the gap height. The fundamental understanding gained increases sensitivity in impedimetric measurements, and can also be used for DMF applications in medical diagnostics, cell manipulation and observation, and condition based maintenance. This research presents an analytical model based on an equivalent circuit of a particle laden droplet. The proposed model predicts that droplet impedance is a function of device geometry, particle size, particle concentration, and the electrical properties of the particles and the surrounding medium.
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Neves Da Silva, Ed, Luiz Maia Neto, and Sergio Pinto Amaral. "LOPA as a PHA complementary tool: a Case Study." In SPE International Conference on Health, Safety and Environment in Oil and Gas Exploration and Production. Society of Petroleum Engineers, 2010. http://dx.doi.org/10.2118/127254-ms.

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Reports on the topic "LOPAC"

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Laurinat, J. E. Analysis of SPRIHTE LOPA flow excursion tests. Office of Scientific and Technical Information (OSTI), February 1993. http://dx.doi.org/10.2172/10176444.

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Checkley, Jr, Herman David M., and Alex W. Assessing Plankton and Particles with an Autonomous Imaging LOPC. Fort Belvoir, VA: Defense Technical Information Center, September 2007. http://dx.doi.org/10.21236/ada573251.

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Sachs, A. D. RELIABILITY of FUEL ASSEMBLY EFFLUENT TEMPERATURES UNDER L0CA/LOPA CONDITIONS. Office of Scientific and Technical Information (OSTI), June 1999. http://dx.doi.org/10.2172/8703.

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Kamps, Charles T. ACSC Quick-Look: LOAC: Time for A Reevaluation? Quick-Look 05-09. Fort Belvoir, VA: Defense Technical Information Center, January 2005. http://dx.doi.org/10.21236/ada430953.

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