Journal articles on the topic 'Lon range contacts'

Afro-Hispanic or bozal Spanish, from the sixteenth century to the early twentieth century, exhibited numerous cases of "epenthetic" nasal consonants, exemplified by Punto Rico < Puerto Rico; limbre < libre 'free'; pincueso < pescuezo 'neck'; and monosyllabic clitics such as lon < lo(s), lan < la(s), and so on. The present study, based on a comparison of Afro-Hispanic (AH) language data from a wide range of regions and time periods, provides alternative models for spontaneous nasalization. The first involves vowel nasalization, analyzed as the linking of a free (nasal) autosegment to the first available vowel of relevant words; Spanish speakers in turn reinterpreted the nasal vowels as a nasal consonant homorganic to the preceding consonant. Cases of apparent word-final nasal epenthesis, invariably involving phrase-internal clitics, resulted from prenasalization of following word-initial obstruents, a well-documented process in Afro-Iberian linguistic contacts. The preference for voiced obstruents to pre-nasalize is attributed to the lack of the normal fricative pronunciation of /b/, /d/, and /g/ in AH speech. In general, Spanish voiced obstruents are pronounced as stops only following nasals. The stop pronunciation of Pol, /d/, and /g/ by AH speakers was reinterpreted as an additional Root node, to which a floating (nasal) autosegment could be linked. AH nasalization generally seems to stem from Africans' underspecification of Spanish vowels and consonants, resulting from the precarious conditions under which Spanish was learned by speakers of various African languages.

Territoriality in animal populations creates spatial structure that is thought to naturally buffer disease invasion. Often, however, territorial populations also include highly mobile, non-residential individuals that potentially serve as disease superspreaders. Using long-term data from the Serengeti Lion Project, we characterize the contact network structure of a territorial wildlife population and address the epidemiological impact of nomadic individuals. As expected, pride contacts are dominated by interactions with neighbouring prides and interspersed by encounters with nomads as they wander throughout the ecosystem. Yet the pride–pride network also includes occasional long-range contacts between prides, making it surprisingly small world and vulnerable to epidemics, even without nomads. While nomads increase both the local and global connectivity of the network, their epidemiological impact is marginal, particularly for diseases with short infectious periods like canine distemper virus. Thus, territoriality in Serengeti lions may be less protective and non-residents less important for disease transmission than previously considered.

The work described was aimed at an analysis of the principal factors affecting the adsorption of 60Co ions onto a peat from Lin Tao County (in the south of Gansu Province, P. R. China). The adsorption of 60Co ions onto the peat was studied as a function of contact time, ratio of solution volume (V) to solid mass (m), pH, ionic strength and the initial concentration of Co ions. It was found that the relative adsorption rate was quite rapid, that adsorption gradually decreased with increasing values of V/m and that ionic strength had a moderate effect on the process. In addition, the pH value strongly influenced the extent of adsorption. Over the high concentration range, the adsorption equilibrium could be described by the Freundlich equation, with this equation being reduced to the Henry equation, i.e. a linear adsorption isotherm, over the low concentration range. No attempt has been made at understanding the mechanism of 60Co ion adsorption onto peat in the present work.

Contact patterns in group-structured populations determine the course of infectious disease outbreaks. Network-based models have revealed important connections between group-level contact patterns and the dynamics of epidemics, but these models typically ignore heterogeneities in within-group composition. Here, we analyse a flexible mathematical model of disease transmission in a hierarchically structured wildlife population, and find that increased variation in group size reduces the epidemic threshold, making social animal populations susceptible to a broader range of pathogens. Variation in group size also increases the likelihood of an epidemic for mildly transmissible diseases, but can reduce the likelihood and expected size of an epidemic for highly transmissible diseases. Further, we introduce the concept of epidemiological effective group size , which we define to be the group size of a hypothetical population containing groups of identical size that has the same epidemic threshold as an observed population. Using data from the Serengeti Lion Project, we find that pride-living Serengeti lions are epidemiologically comparable to a homogeneous population with up to 20 per cent larger prides.

Frequency response function (FRF) data can provide considerably more information on damage in the desired frequency range as compared to modal data extracted from a very limited range around resonances. Among structural health monitoring techniques, FRF-based methods have the potential to locate structural damage. Conventional structural damage detection technology collects structural response data using contact systems, such as displacement or acceleration transducers. However, installing these contact systems can be costly in terms of labor, cost, and time. Several noncontact measurement technologies, such as optical, laser, radar, and GPS, have been developed to overcome these obstacles. Given the rapid advances in optical imaging hardware technology, the use of digital photography in structural monitoring systems has attracted considerable attention. This study develops a displacement FRF-based approach to locate damage to building structures. The proposed damage location index, CurveFRFDI, improves the sensitivity of SubFRFDI, which is a substructure FRF-based damage location index proposed by Lin et al. (2012). Moreover, the feasibility of applying the proposed approach to locate damage to building structures using displacement measured by a digital camera combined with digital image correlation techniques is also investigated in this study. A numerical example and an experimental example are presented to demonstrate the feasibility of using the proposed approach to locate damage to building structures for single and multiple nonadjacent damage locations.

Abstract A recent resurgence of interest in Old Norse linguistic borrowings in Old English has greatly expanded our knowledge of the contact situation between these two speech communities in the early medieval period and beyond. However, there are a significant number of words that have been considered borrowings in the “other” direction, i. e. from Old English to Old Norse, which have not attracted the same amount of attention in current scholarship. Much of this material requires reassessment and this paper provides a case study of two parallel compound formations in both languages – OE bærsynnig [mann]/ON bersynðugr [maðr] (‘one who is openly sinful; publican’), and OE healsbōc/ON hálsbók (‘phylactery, amulet’, lit. ‘neck-book’) – that have traditionally been considered loan translations from Old English to Old Norse with little evidence other than their formation from cognate elements. In the absence of clear-cut linguistic criteria for identifying loan translations between these two closely related languages, this paper draws on a range of literary evidence to argue for a strong likelihood of a relationship between the two compounds. Both words offer important evidence for biblical translation practices, and contribute to our knowledge about the Christianisation of Norse speaking peoples and Anglo-Norse language contact in Viking Age England.

African lions (Panthera leo) are bred in captivity on commercial farms across South Africa and often have close contact with farm staff, tourists, and other industry workers. As transmission of zoonotic diseases occurs through close proximity between wildlife and humans, these commercial captive breeding operations pose a potential risk to thousands of captive lions and to public health. An understanding of pathogens known to affect lions is needed to effectively assess the risk of disease emergence and transmission within the industry. Here, we conduct a systematic search of the academic literature, identifying 148 peer-reviewed studies, to summarize the range of pathogens and parasites known to affect African lions. A total of 63 pathogenic organisms were recorded, belonging to 35 genera across 30 taxonomic families. Over half were parasites (35, 56%), followed by viruses (17, 27%) and bacteria (11, 17%). A number of novel pathogens representing unidentified and undescribed species were also reported. Among the pathogenic inventory are species that can be transmitted from lions to other species, including humans. In addition, 83 clinical symptoms and diseases associated with these pathogens were identified. Given the risks posed by infectious diseases, this research highlights the potential public health risks associated with the captive breeding industry. We recommend that relevant authorities take imminent action to help prevent and manage the risks posed by zoonotic pathogens on lion farms.

Abstract The Pleistocene glacial period shaped the current genetic structure of numerous species. The last glacial dynamics has been proposed to have split the South American sea lion, Otaria flavescens, into two Evolutionarily Significant Units (ESUs), one on each side of the continent. However, previous studies have not provided genetic information on colonies found along 3000 km of coastline of the southernmost limit of the species distribution, where gene flow could occur. We conducted an exhaustive phylogeographical analysis of O. flavescens using a mtDNA marker, including, for the first time, data from colonies living south of latitude 45° S, in the Argentinian provinces of Santa Cruz and Tierra del Fuego. Our results indicated the presence of five Conservation Units across the distribution range of O. flavescens and suggest that the Patagonian population must have expanded about 150 000 BP. We found evidence for gene flow across the entire species range, supporting a scenario of secondary contact in Tierra del Fuego where representatives of the oldest lineages coexist. The presence of gene flow between oceans leads us to reject the assumption of complete reciprocal monophyly for mtDNA between the presumed ESUs, suggesting that the species constitutes a single Evolutionarily Significant Unit.

Fossil evidence of barnacle encrustation of vertebrate bones is reported from the middle Pleistocene Port Orford Formation of southern coastal Oregon. This material includes two associated thoracic vertebrae and a femur referable to the extinct sea lion Proterozetes ulysses that are encrusted by 1400+ individual barnacles (cf. Hesperibalanus hesperius), and a scapula of Zalophus californianus with barnacle attachment scars. In areas, the encrusting barnacles exhibit a roughly bimodal size range, and small barnacles are observed directly encrusting other larger individuals. The size, probable age, and lifespan of extant Hesperibalanus hesperius indicates a minimum period of four to seven months of seafloor exposure between decomposition and burial, although this estimate must be longer because at least two colonization events are represented. Barnacle attachment traces are identified as Anellusichnus circularis. The wide distribution of barnacles on some of these bones suggests these were regularly overturned by bottom currents, which would prevent barnacles from being smothered by prolonged contact with the sediment. Detailed study of barnacle-induced trace fossils on these specimens suggests that episkeletozoans and their traces can be useful sources of data regarding the biostratinomic history of vertebrate fossils.

e19064 Background: Alerta Rosa is a navigation program in Nuevo Leon, Mexico that aims to reduce delays in breast cancer (BC) diagnosis and treatment initiation irrespective of healthcare coverage. Via a call center, a navigator registers each patient’s initial concern and schedules a medical evaluation. This study aimed to assess the implementation of a triage system to guide appointment prioritization for undiagnosed BC patients with abnormal breast findings or imaging studies. Methods: Women that contacted Alerta Rosa were stratified according to their clinical characteristics into three priority groups. Asymptomatic women seeking check-up information were classified as low-priority (“Green”), those with nonspecific symptoms (e.g. mastalgia) or indeterminate image studies (BIRADS 0) as intermediate-priority (“Yellow”), and patients with suspicious symptoms (i.e. palpable mass, nipple retraction or bloody discharge), abnormal image studies (BIRADS 3-5) or an established BC diagnosis as high-priority (“Red”) for an appointment. The triage system’s ability to detect undiagnosed BC was evaluated against the number of patients per category in whom BC was later confirmed. Differences in time from initial contact to first medical evaluation were explored with an independent t-test. Results: From December 2017 to 2019, 561 women with a median age of 44 years (range: 8-89) contacted Alerta Rosa. 369 (66%) reported breast symptoms, 92 (16%) sought check-up information, 68 (12%) had an indeterminate/abnormal image study and 32 (6%) had received a BC diagnosis and wanted a second opinion. Accordingly, 16% of patients were classified as “Green”, 25% “Yellow” and 59% “Red”. The median time from stratification to medical evaluation was 4 days for the “Red” group and 7 days for those in other categories ( p= 0.003). A total of 558 appointments were scheduled, of which 441 (79%) were attended. Excluding those who had received a prior BC diagnosis, 20/299 patients from the “Red” group had BC confirmed (6.7%) compared to 1/138 patient from the “Yellow” (0.7%) and none from the “Green” categories. Therefore, the “Red” category achieved a sensitivity of 95.2% (CI95%: 76.2-99.9%) and specificity of 53.8% (CI95%: 49.3-58.3%) for BC. Conclusions: The triage system adequately identified women with different probabilities of having BC. Thus, the implementation of a stratification system could help identify high risk patients in limited-resource settings where screening programs are ineffective and efforts to prioritize access to medical attention is crucial to achieve early-stage diagnoses.

Aluminium alloy sheets cause many problems in sheet metal forming processes owing to their tendency to gall the surface of the tool. The paper presents a method for the determination of the kinematic friction coefficient of friction pairs. The determination of coefficient of friction (COF) in sheet metal forming requires specialised devices that ‘simulate’ friction conditions in specific areas of the formed sheet. In this article, the friction behaviour of aluminium alloy sheets was determined using the strip drawing test. The 1-mm-thick 6082 aluminium alloy sheets in T6 temper were used as test material. Different values for nominal pressures (4.38, 6.53, 8.13, 9.47, 10.63, and 11.69 MPa) and different sliding speeds (10 and 20 mm/min.) were considered. The change of friction conditions was also realised with several typical oils (hydraulic oil LHL 32, machine oil LAN 46 and engine oil SAE 5W-40 C3) commonly used in sheet metal forming operations. Friction tests were conducted at room temperature (24 °C). The main tribological mechanisms accompanying friction (adhesion, flattening, ploughing) were identified using a scanning electron microscope (SEM). The influence of the parameters of the friction process on the value of the COF was determined using artificial neural networks. The lowest value of the COF was recorded when lubricating the sheet metal surface with SAE 5W40 C3 engine oil, which is characterised as the most viscous of all tested lubricants. In dry friction conditions, a decreasing trend of the COF with increasing contact pressure was observed. In the whole range of applied contact pressures (4.38–11.69 MPa), the value of the COF during lubrication with SAE 5W40 C3 engine oil was between 0.14 and 0.17 for a sliding speed of 10 mm/min and between 0.13 and 0.16 for a sliding speed of 20 mm/min. The value of the COF during dry friction was between 0.23 and 0.28 for a sliding speed of 10 mm/min and between 0.22 and 0.26 for a sliding speed of 20 mm/min. SEM micrographs revealed that the main friction mechanism of 6082-T6 aluminium alloys sheet in contact with cold-work tool steel flattens surface asperities. The sensitivity analysis of the input parameters on the value of COF revealed that oil viscosity has the greatest impact on the value of the COF, followed by contact pressure and sliding speed.

European Environmental Agency estimates that about 120 million people in the EU (over 30% of the total population) are exposed to traffic noise above 55 Ldn dB. It is estimated that 10% of the EU population is exposed to noise associated with the rail traffic. The two main sources of traffic noise comes from vehicles engines and the noise generated in the contact between the wheel and the road. In the latter the considerable part the noise is due to phenomena occurring in a wheel tram – rail system. Therefore, the problem of reducing the noise generated by railway vehicles is the subject of many studies, both experimental and theoretical. Commonly used wheel trams so called "resilient wheels" are equipped with layer made of a resilient material, e.g. rubber, between the tread and the wheel disc. But the monobloc tram wheel is the standard design against which should be carried out the studies on reduction of noise in wheel-rail system. This paper presents the results of calculations related to eigenforms, eigenfrequencies and Frequency Response Function of a three-dimensional model of a monobloc tram wheel. The calculations were carried out using the finite element method. Vibration analysis was performed for the range to 5 kHz. Analysis carried out has shown that the wheel tread plays a more important role in the generation of high-frequency vibrations.

The paper presents imaging properties of the peacock eye optical element. Its abilities for imaging with extended depth of field is illustrated experimentally in monochromatic as well as polychromatic light. According to the obtained results the element makes possible to maintain the acceptable resolution, contrast and brightness of the output images for a wide range of defocusing. Full Text: PDF ReferencesJ. Ares, R. Flores, S. Bara, and Z. Jaroszewicz, "Presbyopia Compensation with a Quartic Axicon", Optom. Vis. Sci. 82, 107 (2005). CrossRef G.-m. Dai, "Optical surface optimization for the correction of presbyopia", Appl. Opt. 45, 4184 (2006). CrossRef Z. Liu, A. Flores, M. R. Wang, and J. J. Yang, "Diffractive infrared lens with extended depth of focus", Opt. Eng. 46, 018002 (1-9) (2007). CrossRef E. E. Garcia-Guerrero, E. R. Mendez, H. M. Escamilla, T. A. Leskova, and A. A. Maradudin, "Design and fabrication of random phase diffusers for extending the depth of focus", Opt. Express 15, 910 (2007). CrossRef J. Lin, J. Liu, J. Ye, and S. Liu, "Design of microlenses with long focal depth based on the general focal length function", J. Opt. Soc. Am. A 24, 1747 (2007). CrossRef N. Davidson, A. A. Friesem, and E. Hasman, "Holographic axilens: high resolution and long focal depth", Opt. Lett. 16, 523 (1991). CrossRef A. Kołodziejczyk, S. Bara, Z. Jaroszewicz, and M. Sypek, "The Light Sword Optical Element?a New Diffraction Structure with Extended Depth of Focus", J. Mod. Opt. 37, 1283 (1990). CrossRef K. Petelczyc, S. Bará, A. Ciro Lopez, Z. Jaroszewicz, K. Kakarenko, A. Kolodziejczyk, and M. Sypek, "Imaging properties of the light sword optical element used as a contact lens in a presbyopic eye model", Opt. Express 19, 25602 (2011). CrossRef K. Kakarenko, I. Ducin, K. Grabowiecki, Z. Jaroszewicz, A. Kolodziejczyk, A. Mira-Agudelo, K. Petelczyc, A. Składowska, M. Sypek, "Assessment of imaging with extended depth-of-field by means of the light sword lens in terms of visual acuity scale", Biomed Opt Express 6, 1738 (2015). CrossRef Z. Jaroszewicz, A. Kołodziejczyk, D. Mouriz, and J. Sochacki, "Generalized Zone Plates Focusing Light into Arbitrary Line Segments", J. Mod. Opt. 40, 601 (1993). CrossRef L. A. Romero, M. S. Millían, Z. Jaroszewicz, A. Kolodziejczyk, "Double peacock eye optical element for extended focal depth imaging with ophthalmic applications", J. Biomed. Opt. 17 046013 (2012). CrossRef

The Ansil massive sulfide deposit occurs at the contact of the underlying Northwest Rhyolite and the overlying Rusty Ridge Andesite, in the lower part of the Central Mine sequence of the Blake River Group. The orebody, which is roughly ellipsoidal in outline and up to 200 m × 150 m across, contained reserves of 1.58 Mt of massive sulfide grading 7.2% Cu, 0.9% Zn, 1.6 g/t Au, and 26.5 g/t Ag. Production began in 1989. Least-altered host rocks are low-K basaltic andesites and low-K rhyolites. These rocks have Zr/Y ratios of ~5 and LaN/YbN ratios of ~2.3, typical of tholeiitic volcanic rocks, although their major-element chemistry is transitional between tholeiitic and calc-alkaline volcanic rocks.The Ansil deposit, which dips ~50° east, is a single orebody comprising two main massive sulfide lenses (up to ~35 m thick) connected laterally via a thinner blanket of massive sulfides, with thin discontinuous but conformable massive magnetite units at the base and top of the orebody. Sulfide ore consists of massive to banded pyrrhotite–chalcopyrite. In the downplunge lens, up to 10 m of massive magnetite are capped by up to 10 m of massive sulfide. Finely banded cherty tuff, with sphalerite–pyrite–chalcopyrite, forms a discontinuous fringe to the deposit.The two main lenses of massive sulfide have the highest contents of Cu, Ag, and Au and are thought to have formed in areas of major hydrothermal input. Altered feeder zones contain either chlorite + chalcopyrite + pyrrhotite ± magnetite, or chlorite + magnetite ± sulfides. Footwall mineralization forms semiconformable zones ~5–10 m thick that directly underlie the orebody and high-angle pipelike zones that extend at least 50 m into the footwall. Ti–Zr–Al plots indicate that almost all altered footwall rocks were derived from a homogeneous rhyolite precursor. Hanging-wall andesites were also altered. Despite some severe alteration, all initial volcanic rock compositions can be readily identified, and thus mass changes can be calculated. Silica has been both significantly added or removed from the footwall, whereas K has been added except in feeder pipes. Oxygen-isotope compositions up to at least 50 m into the hanging wall and footwall are typically depleted in δ18O by 2–6‰. These rocks have gained Fe + Mg and lost Si. Altered samples in general range from light-rare-earth-element (REE) depleted to light-REE enriched, although some samples exhibit little REE modification despite strong alkali depletion. Mineralized volcanic rocks immediately below the orebody are enriched in Eu (as are some Cu-rich sulfides in the orebody).Contact and petrographic relations generally suggest that the main zone of massive magnetite formed by replacement of cp–po-rich sulfides, although local relations are ambiguous. Magnetite formation may reflect waning hydrothermal activity, during which fluids mixed with seawater and became cooler and more oxidized. Cu-rich feeder pipes that cut magnetite-rich footwall indicate a renewal of Cu-sulfide mineralization after magnetite deposition. Chloritic zones with disseminated sulfides occur up to a few hundred metres above the orebody, attesting to continuing hydrothermal activity.

Oxide semiconductors have opened a new era for large-area, flexible and transparent applications. Despite the progresses, a bottleneck issue of oxide thin-film transistors (TFTs) is the instability either under bias stress or when used as a current source. Furthermore, the carrier mobility and current driving capability need to be improved for high-spec displays. It is still hugely challenging to overcome both issues using the conventional device structure and oxide semiconductor materials. Here, we review our recent work on novel oxide TFTs that show a few desirable properties. Rather than using an ohmic metal contact as the source electrode, a high work-function Schottky source contact enables depletion around the TFT source region, which results in intrinsic immunity to the negative bias illumination stress, no obvious short channel effect, and superb current saturation over a wide range of drain voltage1. The flat saturation current gives rise to an extremely high voltage gain reaching 23,000, which is, to the best of our knowledge, the highest gain ever achieved by a solid-state transistor to date. The threshold voltage is also found to remain stable under different drain voltages, in contrast to standard TFTs, which may be useful in larger-area displays where the drain voltages of the drive TFTs can differ2. Furthermore, the depletion provided by the Schottky source electrode allows utilizing semi-metal ITO to replace IGZO as the TFT channel layer, which significantly enhances the carrier mobility and current driving capability. Other related work may also be discussed in the talk including oxide Schottky diodes operating beyond 10 GHz3, oxide TFTs operating beyond 1 GHz4, significantly enhanced carrier mobility by self-assembled monolayer treatment5,6, and CMOS-like oxide-based logic circuits7,8. References Extremely high-gain source-gated transistors, J Zhang, J Wilson, G Auton, Y Wang, M Xu, Q Xin, A Song, Proceedings of the National Academy of Sciences 116 (11), 4843-4848 (2019) Comparative Study of Short-Channel Effects Between Source-Gated Transistors and Standard Thin-Film Transistors, Zhenze Wang, Li Luo, Yiming Wang, Jiawei Zhang, and Aimin Song, IEEE Transactions on Electron Devices, 69(2), 561 - 566 (2022). Flexible indium–gallium–zinc–oxide Schottky diode operating beyond 2.45 GHz, J Zhang, Y Li, B Zhang, H Wang, Q Xin, A Song, Nature communications 6 (1), 1-7 (2015). Amorphous-InGaZnO thin-film transistors operating beyond 1 GHz achieved by optimizing the channel and gate dimensions, Y Wang, J Yang, H Wang, J Zhang, H Li, G Zhu, Y Shi, Y Li, Q Wang, Qian Xin, Zhongchao Fan, Fuhua Yang, Aimin Song, IEEE Transactions on Electron Devices 65 (4), 1377-1382 (2018). Significant Performance Improvement of Oxide Thin‐Film Transistors by a Self‐Assembled Monolayer Treatment, W Cai, J Zhang, J Wilson, J Brownless, S Park, L Majewski, A Song, Advanced Electronic Materials 6 (5), 1901421 (2020). Significant performance enhancement of very thin InGaZnO thin-film transistors by a self-assembled monolayer treatment, W Cai, J Wilson, J Zhang, J Brownless, X Zhang, LA Majewski, A Song, ACS Applied Electronic Materials 2 (1), 301-308 (2020). Complementary integrated circuits based on p-type SnO and n-type IGZO thin-film transistors, Y Li, J Yang, Y Wang, P Ma, Y Yuan, J Zhang, Z Lin, L Zhou, Q Xin, Aimin Song, IEEE Electron Device Letters 39 (2), 208-211 (2017). Thin Film Sequential Circuits: Flip-Flops and a Counter Based on p-SnO and n-InGaZnO, Y Yuan, J Yang, Y Wang, Z Hu, L Zhou, Q Xin, A Song, IEEE Electron Device Letters 42 (1), 62-65 (2020).

As is well known, the design of coastal or offshore structures whether a ship, wave energy device or other fixed or floating structure, needs to consider its operation in a very hostile environment, including heavy storms. For example, an extremely high or steep wave impact on the bow or stern of a moored FPSO may result in a large amount of water on deck. Known as green water, this may cause severe damage to the deck house or other deckside equipment. Thus, there is great need for simulation tools to predict impact loadings and to provide more insight into the physics of local impact phenomena. Published research or prediction work on the water impact problem has mostly related to studies in 2D. For example, Greehow& Lin (1983), Greenhow (1987), Zhao & Faltinsen (1993), Mei et al.(1999) have studied the hydrodynamics of rigid bodies entering water both theoretically and experimentally. More recently, a laboratory investigation of the pressure distribution on a free-falling wedge entering water by Yettou et al.(2006 has been compared a numerical and experimental study carried out by Campbell and Weynberg (1980). Water impact and green water loading in 3D has been simulated by Kleefsman et al. (2005) using a VOF method, which for dam break and water entry problems. In this study, we have developed the AMAZON-3D code for studies of water impact problems involving various 3D rigid solid bodies. The in-house Cartesian cut cell approach has been used to simulate 3D water impact involving both moving rigid solid bodies and the free surface. The Cartesian cut cell method in the AMAZON-3D code is unrestricted in terms of boundary complexity or range of boundary movement. Solid objects are carved out of a background mesh, leaving a set of irregularly shaped cells aligned with the surface boundary. The advantages of the cut cell approach have been outlined previously by Causon et al. (2000, 2001) and Hu et al.(2009) including its flexibility for dealing with arbitrarily complex geometries and moving bodies. There is no requirement to re-mesh globally or even locally for the case of a moving body. All that is required is to update the cut cell data at the body contour for as long as the body motion continues. The AMAZON-3D finite volume code solves the incompressible Navier-Stokes equations in both air and water regions simultaneously treating the free surface as a contact surface in the density field that is captured automatically in a manner analogous to shock capturing in compressible flow. A time-accurate artificial compressibility method and high Godunov-type scheme replaces the pressure correction solver used in other methods (see Qian et al. 2006). We believe that the success of a study of water impact depends ultimately on the problem under consideration and the computer resources available and for each method there is a class of problem for which one method may perform better another. Each method has its own advantages and disadvantages and it is not possible to assert conclusively that one method is uniformly superior. However, we believe we can demonstrate that our method can be used successfully to study real local impact phenomena including the egress of an arbitrary rigid body from air to water or vice versa, the splash zone and entrapment of one fluid into the other. The code has been validated by recourse to a number of test cases including a cone undergoing forced oscillations and water impact of a rigid wedge with constant entry velocity where data and/or analytical results are available for comparison purposes. A range of results including the free surface elevation and force calculations will be presented for the water impact of various 3D rigid bodies.

Macroscopic objects such as fibers and films formed from a combination of nanocarbon materials and polymers have promising electrical [1], thermal [2], thermoelectric [3], and mechanical [4] properties. In particular, composites of nanocarbon and conductive polymers revealed that they can constitute key parts of batteries [1] and thermoelectric generators [3]. In literature, it is possible to find many synthesis methods of this type of material. One of the most common approaches is electrochemical, wherein monomers are polymerized onto the nanocarbon surface due to its highly conducting nature [5]. Polyaniline (PANI) is the oldest and still one of the most popular conductive polymers used in this area. That is because PANI is relatively simple to manufacture at low cost and effort. Moreover, it has good mechanical strength and tunable electrical conductivity, which depend on the kind of PANI. This polymer has three forms: pernigraniline – fully oxidized (purpure/red), emeraldine – partially oxidized (blue/violet), and leucoemeraldine – fully reduced (black) [6]. Emeraldine achieves the highest conductivity when doped with Brøstead acids such as HCl, H2SO4, HBF4 [7]. The goal of this study was to exploit the phenomenon of the hydrophilic surface of carbon nanostructure caused by thermal annealing, described by us previously [8], to electropolymerize aniline onto free-standing films from nanocarbon. The films were manufactured by the wet method [8] using single-walled nanotubes (SWCNTs) exclusively and SWCNTs/graphene nanoplatelet composite. Electropolymerization was used to synthesize PANI on the surface of these materials. To establish the parameters of synthesis of different forms of PANI, voltage ranges between [(-1.6 V) – (1.6 V)] were investigated. The films were tested as a counter and a working electrode in this process. After the synthesis, the composites were investigated by optical and Scanning Electron Microscopy, Raman spectroscopy, Atomic Force Microscopy. Moreover, water contact angles, electrical conductivity values, and Seebeck coefficients were determined. Composites containing all three PANI forms have been synthesized and thoroughly analyzed to elucidate the structure-property relations. Consequently, correlations between the forms of PANI and the characteristics of nanocomposites were established. Depending on the amount and type of PANI on the surface, the character of the nanocarbon films was affected considerably. For example, coating of the material with emeraldine salts enhanced the electrical conductivity of the films by about 60% [9], while simultaneously making the material much stronger. [1] L. Xiao, Y.H. Sehlleier, S. Dobrowolny, F. Mahlendorf, A. Heinzel, C. Schulz, H. Wiggers, Novel Si-CNT/polyaniline nanocomposites as Lithium-ion battery anodes for improved cycling performance, Mater. Today Proc. 4 (2017) S263–S268. doi:10.1016/J.MATPR.2017.09.197. [2] Z. Duan, Y. Luo, Z. Luo, W. Yu, C. Liu, S. Fan, The influence of charging and discharging on the thermal properties of a carbon nanotube/polyaniline nanocomposite electrode, RSC Adv. 9 (2019) 7629–7634. doi:10.1039/C9RA00151D. [3] R. Wu, H. Yuan, C. Liu, J. Le Lan, X. Yang, Y.H. Lin, Flexible PANI/SWCNT thermoelectric films with ultrahigh electrical conductivity, RSC Adv. 8 (2018) 26011–26019. doi:10.1039/c8ra04863k. [4] M.R. Saeb, P. Zarrintaj, Polyaniline/graphene-based nanocomposites, Fundam. Emerg. Appl. Polyaniline. (2019) 165–175. doi:10.1016/B978-0-12-817915-4.00010-5. [5] C. Oueiny, S. Berlioz, F.X. Perrin, Carbon nanotube–polyaniline composites, Prog. Polym. Sci. 39 (2014) 707–748. doi:10.1016/J.PROGPOLYMSCI.2013.08.009. [6] S.C. Rasmussen, The Early History of Polyaniline: Discovery and Origins, An Int. J. Hist. Chem. Subst. 1 (2017) 99–109. doi:10.13128/substantia-30. [7] Q. Qin, Y. Guo, Preparation and characterization of nano-polyaniline film on ITO conductive glass by electrochemical polymerization, J. Nanomater. 2012 (2012). doi:10.1155/2012/519674. [8] D. Janas, G. Stando, Unexpectedly strong hydrophilic character of free-standing thin films from carbon nanotubes, Sci. Rep. 7 (2017) 12274. doi:10.1038/s41598-017-12443-y. [9] G.Stando, P. Stando, P. Chulkin, M. Salhman, M. Lundström, D. Janas, Electropolymerization of aniline onto hydrophilic nanocarbon films (in preparation) G.S. and P.S. would like to thank the Ministry of Science and Higher Education of Poland for financial support of research (under Diamond Grant, grant agreement 0036/DIA/201948). G.S. also would like to thank European Union for thanks for financing the costs of the conference (European Social Fund, grant nr POWR.03.05.00-00-Z305) and National Agency for Academic Exchange of Poland (under the Iwanowska program, grant agreement PPN/IWA/2019/1/00017/UO/00001) for financial support during the stay at the University of Pittsburgh in the USA. G.S. and H.L. acknowledge NSF (CBET-2028826) for partial support of this work. P. S. acknowledges the National Agency for Academic Exchange of Poland (under the Academic International Partnerships program, grant agreement PPI/APM/2018/1/00004) for supporting training in the Aalto University. G.S, P.S and D.J. would like to thank the National Centre for Research and Development, Poland (under the Leader program, grant agreement LIDER/0001/L-8/16/NCBR/2017).

The purpose of the study. The article discusses the research and further development of modern cyber-physical systems of the Internet of Things (CPS IoT). Researchers in the field of psychology, logic, lin- guistics, philosophy, for the study of artificial intelligence, used many different models of this phenomenon of “man”. Currently, the search for new approaches continues. One of the interesting approaches lies in the development of the application of cognitive mechanisms that a person uses in solving everyday tasks. The application of methods of gestalt processing of states of the CPS IoT worldview is considered to solve an ever-expanding range of tasks. Hybrid or, as they are also called, combined models can be used for gestalt processing to solve various tasks of cyber-physical systems of the Internet of Things related to forecasting indexes of the functioning of socio-economic systems. To do this, you can use a long-known approach to hybrid- ization, the essence of which is to split the entire set of initial data into subsets of initial data. The main stages of gestalt processing and one of the initial stages – the formation of a need are shown. The significant moments of the organization of interaction of CPS IoT modules with the environment are presented. In the demo example, the CPS IoT of the estate museum is considered. The main elements of the formation of the need for CPS IoT in organizing and conducting excursions are described.Materials and methods of research. To solve problems within the framework of gestalt processing of CPS IoT, new methods and developments of specialists in the field of intelligent systems are re- quired. Hybrid, combined models can be used for gestalt processing for various tasks of cyber-physical systems of the Internet of Things related to forecasting indexes of the functioning of socio-economic systems. You can use a long-known approach to hybridization, the essence of which is to split the entire set of initial data into subsets of initial data. It is possible to use gestalt processing within the framework of a holistic approach when splitting into subsets of the initial data - clusters. The cognitive approach uses sensory images, concept-representations, concept-scenarios and gestalts. The CPS IoT gestalt can be considered as one of the approaches to solving modern problems within the framework of Industry 4.0 technology. The article describes intelligent cyber-physical systems using methods of gestalt processing of their states - a picture of the world. Approaches to the use of needs and one of the many approaches to the formation of the needs of cyber-physical systems of the Internet of Things are considered.Results. Within the framework of the cognitive approach, con- cepts-representations of CPS IoT for arising (emerging) needs and their fixation are proposed. After fixing the need, as the main stages of its formation, the following are highlighted: concretization of the event, collection of relevant initial data for the need; formation (folding of perceived information) of the gestalt: checking the state of the necessary elements, searching for resources in the environment; organization of contact with the environment. These stages of the formation of the need relate to CPS IoT of any nature and are fo- cused on any tasks of the Internet of Things. It is advisable to save scenarios for the formation of needs for possible reuse in the future. The demo example shows an example of the formation of a need for CPS IoT during an excursion.Conclusion. The article considers a cognitive approach related to the use and development of intelligent cyber-physical systems for the Internet of Things and the Internet of everything. The main stages of the formation of the need for CPS IoT are considered. The implementation of the proposed approach, the development and use of gestalt concepts will allow reflecting CPS IoT with new emergent properties.

Cell-type specific transcriptional programs are regulated by the activity of tissue-specific transcription factors and enhancer elements within structurally defined topologically associating domains (TADs). The coordinated and dynamic changes in chromatin architecture are highly regulated as transcriptional output is influenced by chromatin accessibility, histone modification, promoter enhance interactions, and recruitment of transcriptional co-activator complexes. The genes which contribute to transcriptional regulation, including members of the cohesin complex, are frequently mutated in human cancers, including leukemias, Ewing sarcoma, and glioblastoma multiforme. Despite this, the mechanistic role of STAG2 in gene regulation, hematopoietic function, and tumor suppression has not been delineated. We show that somatic Stag2 deletion in hematopoietic stem/progenitor cells (HSPC) results in altered hematopoietic function, increased self-renewal, and impaired differentiation across all three lineages consistent with myelodysplasia. Chromatin immunoprecipitation sequencing of Stag2-deficient HSPCs revealed that Stag2 and Stag1 have both shared and non-redundant cistromes with Stag1/2 common binding sites enriching at TAD boundaries with CTCF occupancy. This maintains TAD integrity in the setting of Stag2 loss of function which we confirmed with Hi-C chromosome capture. High resolution of the Hi-C data at 10kB resolution identified a specific role for Stag2, but not Stag1, in maintaining short-range chromatin interactions, specifically at genes with PU.1 and IRF8 motifs. While co-deletion of Stag2 and Stag1 resulted in synthetic lethality, Stag2 loss alone resulted in decreased chromatin accessibility and reduced transcriptional output at key PU.1 target loci involved in lineage-specification, including reduced Ebf1 and Pax5 expression resulting in impaired B-lineage differentiation. We investigated whether expression of PU.1 could overcome the non-permissive chromatin state at these key downstream targets and rescue hematopoietic differentiation; however, PU.1 expression could not restore Ebf1 expression or B cell differentiation and did not attenuate the serial replating capacity of Stag2 deficient hematopoietic stem/progenitor cells (HSPCs). Given this transcriptional "choke-point" we investigated whether expression of Ebf1 would have a more significant impact on Stag2 deficient cells. Indeed Ebf1 rescue restored B cell colony formation/differentiation in vitro and in vivo and abrogated serial replating of Stag2 deficient HSPCs. These data highlight the non-hierarchical and non-redundant relationship between transcription factors and chromatin architecture and demonstrate a key role for Stag2-regulated local interactions in transcription factor output and hematopoietic differentiation. Nonetheless, the mechanistic underpinnings of the structural basis for transcriptional regulation remain associative. We have recently been able to reduce the cell input for Hi-C assays such that we can now analyze the chromatin architecture of purified populations and model the structural transition from Lin- Sca-1+ Kit+ hematopoietic stem cells to committed granulocyte macrophage precursor cells both in normal hematopoiesis and in the Stag2 deficient setting. Previous studies using in vitro systems have shown that complete cohesin depletion results in the loss of structural loop components; however, cohesin levels are reduced, but not absent, in cancer cells. As such, our studies highlight a key role for locus-specific alterations in gene regulation and DNA interactions in Stag2 deficient cells, which results in altered gene expression and contributes to transformation. Taken together, these data identify a key role for Stag2 loss in transcriptional dysregulation distinct from its shared role with Stag1 in chromosomal segregation. Moreover, we illustrate a critical link between cohesin, chromosomal contacts, and gene regulation that contributes to hematopoietic transformation. Disclosures Viny: Mission Bio: Other: Sponsored travel; Hematology News: Membership on an entity's Board of Directors or advisory committees. Dekker:Arima Genomics: Membership on an entity's Board of Directors or advisory committees. Levine:Imago Biosciences: Membership on an entity's Board of Directors or advisory committees; Amgen: Honoraria; Lilly: Honoraria; Gilead: Consultancy; Novartis: Consultancy; Prelude Therapeutics: Research Funding; Roche: Consultancy, Research Funding; Celgene: Consultancy, Research Funding; C4 Therapeutics: Membership on an entity's Board of Directors or advisory committees; Isoplexis: Membership on an entity's Board of Directors or advisory committees; Qiagen: Membership on an entity's Board of Directors or advisory committees; Loxo: Membership on an entity's Board of Directors or advisory committees.

Abstract Background: Tissue factor pathway inhibitor (TFPI) plays a major role in regulation of tissue factor (TF)-initiated coagulation in a FXa-dependent manner. Andexanet alfa (AnXa) is a modified, recombinant, catalytically inactive form of human FXa being developed to reverse the anticoagulant activity of FXa inhibitors in patients during episodes of major bleeding. As a modified FXa, AnXa retained high binding affinity to fXa inhibitors, including TFPI. Our previous in vitro studies demonstrated that AnXa reverses rivaroxaban-induced inhibition of thrombin generation (TG) initiated by either the extrinsic or intrinsic pathways. However, the thromboelastography (TEG) results showed that AnXa-TFPI interaction enhanced thrombin formation at low TF conditions, which suggested potential increase in the fibrin degradation product in the presence of recombinant tissue plasminogen activator (rtPA) (Lu, G., et al, ASH2016). In the current study, we further characterized the differential effect of AnXa on thrombin formation and clot lysis via the two different pathways, and correlated the TG and TEG parameters with the coagulation markers in the TF-initiated reaction in plasma, including prothrombin fragment (F1+2), thrombin-antithrombin complex (TAT), and fibrin degradation product (D-dimer). Methods: TF-initiated TG in human plasma was measured using a calibrated automated thrombogram (TF-CAT) and the PPP-reagent (5 pM TF, Diagnostica Stago). Non-TF-initiated TG was measured using CAT and an aPTT reagent, Actin FS (Siemens Healthcare) as the activator (Actin FS-CAT). Clot formation was measured in plasma using a TEG 5000 analyzer (Haemonetics). TF (1 pM) and Actin FS (1:240 final dilution) were used as the activators with or without rtPA (150 ng/mL). Time-dependent thrombin formation and clot lysis were monitored by quenching samples at various times (0, 2.5, 5, 10, 15, 30, 60 min) following initiation of the reaction by TF (1 pM) in plasma with or without rtPA (150 ng/mL) and AnXa (4.0 µM). Corn trypsin inhibitor (CTI, 50 µg/mL) was added to block contact activation. F1+2, TAT and D-dimer were quantified according to manufacturers' recommendations. Results: The effect of AnXa on TF-CAT and Actin FS-CAT were compared side-by-side with AnXa at low (0 - 20 nM) and high (0 - 4.0 µM) concentration ranges. AnXa (5 nM) increased TF-CAT parameters sensitive to TFPI activity (i.e., peak thrombin), with less effect on endogenous thrombin potential (ETP). No further increase in these parameters was observed at AnXa therapeutic concentrations (e.g., 2.0 and 4.0 µM), consistent with the expected effect of AnXa on the activity of TFPI, which is present at low plasma concentration (~2.4 nM). In contrast, AnXa did not significantly affect the Actin FS-CAT parameters. The effect of AnXa-TFPI interaction on coagulation and fibrinolytic pathways in human plasma were compared using TEG, with or without rtPA. AnXa (4.0 µM) reduced the TF-TEG-R parameter (lag time, equivalent to clotting time) and resulted in an increased area under the fibrinolytic profile with rtPA as previously shown. In contrast, AnXa and the buffer controls had similar profiles in the Actin FS-TEG assay under similar conditions, with or without rtPA (150 ng/mL) in the current studies, suggesting lack of an AnXa-TFPI effect. We further investigated the effect of AnXa-TFPI interaction on coagulation markers following TF (1pM)-initiated thrombin formation in plasma. AnXa (4 µM) increased F1+2 and TAT levels at early time points following initiation (2.5, 5, 10, 15 min), but had no difference at or after 30 min, compared to buffer control. A similar pattern was observed for D-dimer level when exogenous rtPA (150 ng/mL) was added. Compared to buffer control, AnXa caused elevation of D-dimer level at early time points but had no difference at or after 30 min following initiation of the reaction. Conclusions: AnXa-TFPI interaction can modulate the TF/TFPI activity in the initiation phase of the extrinsic coagulation pathway and increase parameters sensitive to TFPI activity in the TF-CAT and TF-TEG assays, but has minimal effect on the later phase of the reactions and the overall thrombin formation potential. Consistent with clinical observations, AnXa-TFPI interaction increased the F1+2 and TAT levels, as well as D-dimer level in the presence of rtPA in the early phase of the reaction following initiation of the reaction by TF. Disclosures Lu: Portola Pharmaceuticals, Inc.: Employment. Lin: Portola Pharmaceuticals, Inc.: Employment. Curnutte: 3-V Biosciences: Equity Ownership; Sea Lane Biotechnologies: Consultancy; Portola Pharmaceuticals, Inc.: Employment, Equity Ownership, Patents & Royalties. Conley: Portola Pharmaceuticals, Inc.: Employment, Equity Ownership, Patents & Royalties.

Summary Evaluation and improvement of sweep efficiency are important for miscible displacement of medium-viscosity oils. A high-pressure quarter-five-spot cell was used to conduct multicontact miscible (MCM) water-alternating-gas (WAG) displacements at reservoir conditions. A dead reservoir oil (78 cp) was displaced by ethane. The minimum miscibility pressure (MMP) for ethane with the reservoir oil is approximately 4.14 MPa (600 psi). Gasflood followed by waterflood improves the oil recovery over waterflood alone in the quarter five-spot. As the pressure decreases, the gasflood oil recovery increases slightly in the pressure range of 4.550-9.514 MPa (660-1,380 psi) for this undersaturated viscous oil. WAG improves the sweep efficiency and oil recovery in the quarter five-spot over the continuous gas injection. WAG injection slows down gas breakthrough. A decrease in the solvent amount lowers the oil recovery in WAG floods, but significantly more oil can be recovered with just 0.1 pore volume (PV) solvent (and water) injection than with waterflood alone. Use of a horizontal production well lowers the sweep efficiency over the vertical production well during WAG injection. Sweep efficiency is higher for the nine-spot pattern than for the five-spot pattern during gas injection. Sweep efficiency during WAG injection increases with the WAG ratio in the five-spot model. Introduction As the light-oil reservoirs get depleted, there is increasing interest in producing more-viscous-oil reservoirs. Thermal techniques are appropriate for heavy-oil reservoirs. But gasflooding can play an important role in medium-viscosity-oil (30-300 cp) reservoirs and is the subject of this paper. Roughly 20 billion to 25 billion bbl of medium-weight- to heavy-weight-oil deposits are estimated in the North Slope of Alaska. Approximately 10 billion to 12 billion bbl exist in West Sak/Schrader Bluff formation alone (McGuire et al. 2005). Miscible gasflooding has been proved to be a cost-effective enhanced oil recovery technique. There are approximately 80 gasflooding projects (CO2, flue gas, and hydrocarbon gas) in the US and approximately 300,000 B/D is produced from gasflooding, mostly from light-oil reservoirs (Moritis 2004). The recovery efficiency [10-20% of the original oil in place (OOIP)] and solvent use (3-12 Mcf/bbl) need to be improved. The application of miscible and immiscible gasflooding needs to be extended to medium-viscosity-oil reservoirs. McGuire et al. (2005) have proposed an immiscible WAG flooding process, called viscosity-reduction WAG, for North Slope medium-visocisty oils. Many of these oils are depleted in their light-end hydrocarbons C7-C13. When a mixture of methane and natural gas liquid is injected, the ethane and components condense into the oil and decrease the viscosity of oil, making it easier for the water to displace the oil. From reservoir simulation, this process is estimated to enhance oil recovery compared to waterflood from 19 to 22% of the OOIP, which still leaves nearly 78% of the OOIP. Thus, further research should be directed at improving the recovery efficiency of these processes for viscous-oil reservoirs. Recovery efficiency depends on microscopic displacement efficiency and sweep efficiency. Microscopic displacement efficiency depends on pressure, (Dindoruk et al. 1992; Wang and Peck 2000) composition of the solvent and oil (Stalkup 1983; Zick 1986), and small-core-scale heterogeneity (Campbell and Orr 1985; Mohanty and Johnson 1993). Sweep efficiency of a miscible flood depends on mobility ratio (Habermann 1960; Mahaffey et al. 1966; Cinar et al. 2006), viscous-to-gravity ratio (Craig et al. 1957; Spivak 1974; Withjack and Akervoll 1988), transverse Peclet number (Pozzi and Blackwell 1963), well configuration, and reservoir heterogeneity, (Koval 1963; Fayers et al. 1992) in general. The effect of reservoir heterogeneity is difficult to study at the laboratory scale and is addressed mostly by simulation (Haajizadeh et al. 2000; Jackson et al. 1985). Most of the laboratory sweep-efficiency studies (Habermann 1960; Mahaffey et al. 1966; Jackson et al. 1985; Vives et al. 1999) have been conducted with first-contact fluids or immiscible fluids at ambient pressure/temperature and may not be able to respresent the displacement physics of multicontact fluids at reservoir conditions. In fact, four methods are proposed for sweep improvement in gasflooding: WAG (Lin and Poole 1991), foams (Shan and Rossen 2002), direct thickeners (Xu et al. 2003), and dynamic-profile control in wells (McGuire et al. 1998). To evaluate any sweep-improvement methods, one needs controlled field testing. Field tests generally are expensive and not very controlled; two different tests cannot be performed starting with identical initial states, and, thus, results are often inconclusive. Field-scale modeling of compositionally complex processes can be unreliable because of inadequate representation of heterogeneity and process complexity in existing numerical simulators. There is a need to conduct laboratory sweep-efficiency studies with the MCM fluids at reservoir conditions to evaluate various sweep-improvement techniques. Reservoir-conditions laboratory tests can be used to calibrate numerical simulators and evaluate qualitative changes in sweep efficiency. We have built a high-pressure quarter-five-spot model where reservoir-conditions multicontact WAG floods can be conducted and evaluated (Dao et al. 2005). The goal of this paper is to evaluate various WAG strategies for a model oil/multicontact solvent in this high-pressure laboratory cell. In the next section, we outline our experimental techniques. The results are summarized in the following section.

Abstract Background and Rationale: Axi-cel is a standard-of-care treatment for relapsed or refractory LBCL after 2 or more lines of systemic therapy. Some patients who would have been ineligible for ZUMA-1 criteria due to comorbidities have been treated with axi-cel in the real-world setting. We interrogated a large registry of patients treated with standard-of-care axi-cel for a definitive report on the impact of age or certain comorbidities on safety and efficacy outcomes after axi-cel infusion. Methods: From October 2017 to August 2020, 1500 patients were enrolled in the postapproval safety observational study of axi-cel treated at 79 centers. Patients eligible for the protocol and followed up for at least 6 months with complete data entry by the time of analyses were included. One patient rescinded consent and 1 was deemed ineligible. Patients previously treated with immune effector cell therapy (n=31), with incomplete follow-up reporting (n=92), or alive but last contacted &lt;180 days postinfusion (n=32) were excluded from analysis. Main efficacy outcomes included complete and objective response rates (CR and ORR), duration of response (DOR), progression-free and overall survival (PFS and OS). Safety endpoints of interest included cytokine release syndrome (CRS) and immune effector cell-associated neurotoxicity syndrome (ICANS). Outcomes were assessed and compared by age and preselected coexisting disease or organ impairment within 3 months prior to the infusion included in the hematopoietic cell transplantation comorbidity index. Multivariate logistic and Cox regression models were used to assess the impact of age or coexistent organ dysfunction on outcomes via odds ratio (OR) or hazard ratio (HR) and their 95% CIs. Results A total of 1343 patients were included in the analysis with a median 25.1 months (range, 10.3-42.7 months) for potential follow-up, defined from infusion to data cutoff date, and a median 11.8 months (range, 0.1-38.8 months) for actual follow-up time, defined from infusion to date of death or last contact. Of these, 38% were 65 years or older (median 62 years), 4% had a performance score ≥2, 13% had cardiac comorbidities, 16% had a prior cancer, 9% were obese, 2% had moderate to severe hepatic comorbidities, and 2% had renal comorbidities. Transformed lymphoma, double or triple-hit by fluorescence in situ hybridization, prior autologous transplant and refractory disease were present in 28%, 15%, 27% and 66%, respectively. Bridging therapy was administered in 21% of patients. Overall, ORR was 74%, (CR 56%), and probabilities of DOR, PFS, and OS at 18 months were 61% (95% CI, 57-65%), 42% (95% CI, 39-45%) and 52% (95% CI, 49-55%), respectively. Overall rates of CRS and ICANS were 83% and 55%, respectively. ORR was 78% (CR 62%, median OS 17.5 [95% CI, 16.0-not evaluated (NE)] months) for patients ≥65 years, ORR 57% (CR 29%; median OS 4.3 [95% CI, 2.5-8.3] months) for patients with hepatic dysfunction, ORR 70% (CR 43%; median OS 8.9 [95% CI, 3.8-NE] months) for patients with renal dysfunction; and ORR 47% (CR 20%, median OS 4 [95% CI, 2.6-6.9] months) for patients with Eastern Cooperative Oncology Group (ECOG) 2 or 3. Multivariate analyses indicated that advanced age (≥65 years vs &lt;65 years; OR 1.38; 95% CI, 1.05-1.83), moderate to severe pulmonary disease (yes vs no; OR 0.74; 95% CI, 0.55-0.98), and ECOG (2-3 vs 0-1; OR 0.31, 95% CI, 0.18-0.53) impacted ORR (Table). Age ≥65 years did not have an impact on survival (HR 1.05, 95% CI, 0.88-1.26), although it was associated with CRS (OR 1.42, 95% CI, 1.03-1.96) and ICANS (OR 1.78, 95% CI, 1.39-2.28). Coexistent cardiac disease (HR 1.44, 95% CI, 1.12-1.83), renal disease (HR 2.13, 95% CI, 1.32-3.46) and hepatic dysfunction (HR 2.65, 95% CI, 1.69-4.14) had an impact on OS, but not on response, CRS, and ICANS. ECOG significantly impacted all efficacy outcomes. None of the other comorbidities tested significantly impacted outcomes. Conclusion: Advanced age (≥65 years) was not associated with worse efficacy outcomes after axi-cel, despite higher rates of CRS and ICANS, which require closer monitoring. Performance status rather than age should be accounted for in patient selection and treatment decisions with axi-cel. Most coexistent organ dysfunctions have no clinically significant impact on axi-cel objective response and safety outcomes. Figure 1 Figure 1. Disclosures Locke: Moffitt Cancer Center: Patents & Royalties: field of cellular immunotherapy; Allogene Therapeutics: Consultancy, Other: Scientific Advisory Role, Research Funding; Amgen: Consultancy, Other: Scientific Advisory Role; Bluebird Bio: Consultancy, Other: Scientific Advisory Role; EcoR1: Consultancy; Emerging Therapy Solutions: Consultancy; Gerson Lehrman Group: Consultancy; Iovance Biotherapeutics: Consultancy, Other: Scientific Advisory Role; Takeda: Consultancy, Other; Novartis: Consultancy, Other, Research Funding; Wugen: Consultancy, Other; GammaDelta Therapeutics: Consultancy, Other: Scientific Advisory Role; Umoja: Consultancy, Other; Cellular Biomedicine Group: Consultancy, Other: Scientific Advisory Role; Calibr: Consultancy, Other: Scientific Advisory Role; Cowen: Consultancy; BMS/Celgene: Consultancy, Other: Scientific Advisory Role; Legend Biotech: Consultancy, Other; Kite, a Gilead Company: Consultancy, Other: Scientific Advisory Role, Research Funding; Janssen: Consultancy, Other: Scientific Advisory Role. Jacobson: Axis: Speakers Bureau; Nkarta: Consultancy, Honoraria; Pfizer: Consultancy, Honoraria, Other: Travel support, Research Funding; AbbVie: Consultancy, Honoraria; Celgene: Consultancy, Honoraria, Other: Travel support; Novartis Pharmaceuticals Corporation: Consultancy, Honoraria, Other: Travel support; Precision Biosciences: Consultancy, Honoraria, Other: Travel support; Kite, a Gilead Company: Consultancy, Honoraria, Other: Travel support; Lonza: Consultancy, Honoraria, Other: Travel support; Humanigen: Consultancy, Honoraria, Other: Travel support; Clinical Care Options: Speakers Bureau. Ma: Kite, a Gilead Company: Current Employment. Dong: Kite, a Gilead Company: Current Employment. Hu: Kite, a Gilead Company: Research Funding; Novartis: Research Funding; Celgene: Research Funding. Siddiqi: BMS: Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; Oncternal: Research Funding; Janssen: Speakers Bureau; Juno Therapeutics: Membership on an entity's Board of Directors or advisory committees, Research Funding; Kite Pharma: Membership on an entity's Board of Directors or advisory committees, Research Funding; BeiGene: Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; AstraZeneca: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Celgene: Membership on an entity's Board of Directors or advisory committees; Pharmacyclics LLC, an AbbVie Company: Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; TG Therapeutics: Research Funding. Ahmed: Seagen: Research Funding; Tessa Therapeutics: Membership on an entity's Board of Directors or advisory committees, Research Funding; Merck: Research Funding; Xencor: Research Funding. Ghobadi: Atara: Consultancy; Wugen: Consultancy; Amgen: Consultancy, Research Funding; Kite, a Gilead Company: Consultancy, Honoraria, Research Funding; Celgene: Consultancy. Miklos: Pharmacyclics: Patents & Royalties; Kite, a Gilead Company, Amgen, Atara, Wugen, Celgene, Novartis, Juno-Celgene-Bristol Myers Squibb, Allogene, Precision Bioscience, Adicet, Pharmacyclics, Janssen, Takeda, Adaptive Biotechnologies and Miltenyi Biotechnologies: Consultancy; Pharmacyclics, Amgen, Kite, a Gilead Company, Novartis, Roche, Genentech, Becton Dickinson, Isoplexis, Miltenyi, Juno-Celgene-Bristol Myers Squibb, Allogene, Precision Biosciences, Adicet, Adaptive Biotechnologies: Research Funding; Adaptive Biotechnologies, Novartis, Juno/Celgene-BMS, Kite, a Gilead Company, Pharmacyclics-AbbVie, Janssen, Pharmacyclics, AlloGene, Precision Bioscience, Miltenyi Biotech, Adicet, Takeda: Membership on an entity's Board of Directors or advisory committees. Lin: Kite, a Gilead Company: Consultancy, Research Funding; Celgene: Consultancy, Research Funding; Legend: Consultancy; Vineti: Consultancy; Sorrento: Consultancy; Gamida Cell: Consultancy; Janssen: Consultancy, Research Funding; Juno: Consultancy; Bluebird Bio: Consultancy, Research Funding; Novartis: Consultancy; Merck: Research Funding; Takeda: Research Funding. Perales: Bristol-Myers Squibb: Honoraria; Takeda: Honoraria; Nektar Therapeutics: Honoraria, Other; Miltenyi Biotec: Honoraria, Other; MorphoSys: Honoraria; NexImmune: Honoraria; Sellas Life Sciences: Honoraria; Cidara: Honoraria; Merck: Honoraria; Omeros: Honoraria; Servier: Honoraria; Celgene: Honoraria; Novartis: Honoraria, Other; Kite/Gilead: Honoraria, Other; Incyte: Honoraria, Other; Medigene: Honoraria; Karyopharm: Honoraria; Equilium: Honoraria. Lunning: Daiichi-Sankyo: Consultancy; TG Therapeutics: Consultancy; Kite, a Gilead Company: Consultancy; ADC Therapeutics: Consultancy; Spectrum: Consultancy; Beigene: Consultancy; Celgene, a Bristol Myers Squibb Co.: Consultancy; Legend: Consultancy; AbbVie: Consultancy; AstraZeneca: Consultancy; Morphosys: Consultancy; Verastem: Consultancy; Novartis: Consultancy; Myeloid Therapeutics: Consultancy; Janssen: Consultancy; Acrotech: Consultancy; Kyowa Kirin: Consultancy; Karyopharm: Consultancy. Hill: Gentenech: Consultancy, Honoraria, Research Funding; Incyte/Morphysis: Consultancy, Honoraria, Research Funding; Beigene: Consultancy, Honoraria, Research Funding; Pfizer: Consultancy, Honoraria; Kite, a Gilead Company: Consultancy, Honoraria, Other: Travel Support, Research Funding; Karyopharm: Consultancy, Honoraria, Research Funding; AstraZenica: Consultancy, Honoraria; AbbVie: Consultancy, Honoraria, Research Funding; Novartis: Consultancy, Honoraria, Research Funding; Epizyme: Consultancy, Honoraria; Celgene (BMS): Consultancy, Honoraria, Research Funding. Nikiforow: Kite/Gilead: Other: ad HOC Advisory Boards; Novartis: Other: ad Hoc Advisory Boards; Iovance: Other: ad Hoc Advisory Boards; Glaxo Smith Kline (GSK): Other: ad Hoc Advisory Boards. Xu: Gilead Sciences: Other: stock or other ownership ; Kite, A Gilead Company: Current Employment. Pasquini: Bristol Myers Squibb: Consultancy, Research Funding; Novartis: Research Funding; Kite Pharma: Research Funding; GlaxoSmithKline: Research Funding.

International visitor surveys (IVS) are traditionally designed to provide destinations with marketing data and intelligence. The New Zealand Tourism Research Institute has been developing new approaches to IVS implementation and data collection in the Pacific Islands that can provide a much richer source of information [1]. The research outlined here is the first to utilise an IVS to explore the positioning of cuisine in the culinary identity of a destination – specifically, the cuisine of the Cook Islands. The Cook Islands is known primarily for its sun, sea and sand features, rather than its culinary attributes. Drawing on data mining of the Cook Islands IVS (2012–2016) and a web audit of destination websites and menus, this paper considers the positioning of food and food-related activities within the Pacific nation’s tourism experience. National tourism organisations are increasingly seeking competitive advantage by utilising their local cuisines as tourist attractions. Research suggests that distinctive local cuisines can act as both a tourism attraction, and as a means of shaping the identity of a destination [2, 3]. In addition to providing an important source of marketable images, local cuisine can also provide a unique experience for tourists. This reinforces the competitiveness and sustainability of the destination [2]. The cuisine of the Cook Islands has come up repeatedly in recommendations for how the country can grow its tourism revenue. Recommendations have been made to improve the food product on offer, develop a distinctive Cook Islands cuisine based on fresh, local produce, and to promote a Cook Islands cuisine experience [4, 5], and to use these to market the Cook Islands as a destination for local food tourism experiences [4]. Despite these recommendations, Cook Island cuisine features less prominently than stereotypical sun, sea, and sand marketing images, and little is known about tourists’ perceptions of and satisfaction with food and food-related activities [6]. Our research addresses this gap by mining IVS data to gain a deeper understanding of tourists’ experiences and perceptions of food in the Cook Islands and assessing whether local food can be positioned as means of creating a unique destination identity. Two methods were used to develop a picture of where food sits in the Cook Islands tourist experience: one focussed on tourist feedback; and the other focused on how food is portrayed in relevant online media. Analysis of all food-related data collected as part of the national IVS between 1 April 2012 and 30 June 2016 was conducted (N = 10,950). A web audit also focused on how food is positioned as part of the Cook Islands tourism product. After identifying the quantitative food-related questions in the IVS, satisfaction with these activities was analysed. Qualitative comments related to food experiences were also examined. The results suggest that participation in food-related activities is generally a positive feature of the visitor experience. The web-audit revealed, however, that food is not a salient feature in the majority of Cook Islands-related websites, and when food did feature, it tended to be oriented towards international cuisine with a ‘touch of the Pacific’ rather than specifically Cook Islands cuisine. This reinforced findings from the IVS data mining that Cook Islands food is presented as a generic tropical ‘seafood and fruit’ cuisine that, largely, lacks the defining and differentiating features of authentic Cook Island cuisine. High participation rates in food-related activities and overall positive evaluations by visitors emerged from the IVS data, yet a dearth of images and information on the country’s food suggests that the Cook Islands is not exploiting its cuisine and food experiences to their full potential. As a direct result of this secondary analysis of IVS data, which highlighted the importance of and potential for food-related activities, the Cook Islands Government is now actively addressing this gap by developing a range of food-related resources and information that can better link tourism to local cuisine. In addition to developing a greater presence of local food in online resources, the Cook Islands Tourism Corporation has also taken on board the messages from the IVS to drive the development of Takurua [7] – an initiative to develop and document local, traditional cuisine and share it with the world. This approach is part of a broader ongoing effort to differentiate the Cook Islands from other South Pacific destinations through its unique cultural attributes. Data mining and secondary analysis of IVS data has not been restricted to the identification of food-related opportunities. Secondary analysis of IVS data in the Pacific has also been used to investigate the impact of other niche markets such as events [8] and to gauge the impact of environmental incidents, for example Cyclone Pam in Vanuatu [9] and algal bloom in the Cook Islands [10], thus reinforcing that IVS data are a rich source of information and are indeed more than just numbers. Corresponding author Tracy Berno can be contacted at tracy.berno@aut.ac.nz References (1) New Zealand Tourism Research Institute (NZTRI). Cook Islands Resources and Outputs; NZTRI: Auckland. http://www.nztri.org.nz/cook-islands-resources (accessed Jun 10, 2019). (2) Lin, Y.; Pearson, T.; Cai, L. Food as a Form of Destination Identity: A Tourism Destination Brand Perspective. Tourism and Hospitality Research 2011, 11, 30–48. https://doi.org/10.1057/thr.2010.22 (3) Okumus, F.; Kock, G.; Scantlebury, M. M.; Okumus, B. Using Local Cuisines when Promoting Small Caribbean Island Destinations. Journal of Travel & Tourism Marketing 2013, 30 (4), 410–429. (4) Food and Agricultural Organization (FAO). Linking Farmers to Markets: Realizing Opportunities for Locally Produced Food on Domestic and Tourist Markets in Cook Islands. FAO Sub-regional Office of the Pacific Islands: Apia, Samoa, 2014. (5) United Nations. “Navigating Stormy Seas through Changing winds”: Developing an Economy whilst Preserving a National Identity and the Modern Challenges of a Small Island Developing State. The Cook Islands National Report for the 2014 Small Islands Developing States (SIDS) Conference and post 2015 Sustainable Development Goals (SDGs). https://sustainabledevelopment.un.org/content/documents/1074217Cook%20Is%20_%20Final%20NATIONAL%20SIDS%20Report.pdf (accessed Jun 10, 2019). (6) Boyera, S. Tourism-led Agribusiness in the South Pacific Countries; Technical Centre for Agriculture and Rural Cooperation (CTA): Brussels, 2016. (7) Cook Islands Tourism Corporation (CITC). Takurua: Food and Feasts of the Cook Islands; CITC: Avarua, Cook Islands, 2018. (8) Thorburn, E.; Milne, S.; Histen, S.; Sun, M.; Jonkers, I. Do Events Attract Higher Yield, Culturally Immersive Visitors to the Cook Islands? In CAUTHE 2016: The Changing Landscape of Tourism and Hospitality: The Impact of Emerging Markets and Emerging Destinations; Scerri, M., Ker Hui, L., Eds.; Blue Mountains International Hotel Management School: Sydney, 2016; pp 1065–1073. (9) Sun, M.; Milne, S. The Impact of Cyclones on Tourist Demand: Pam and Vanuatu. In CAUTHE 2017: Time for Big Ideas? Re-thinking the Field for Tomorrow; Lee, C., Filep, S., Albrecht, J. N., Coetzee, W. JL, Eds.; Department of Tourism, University of Otago: Dunedin, 2017; pp 731–734. (10) Thorburn, E.; Krause, C.; Milne, S. The Impacts of Algal Blooms on Visitor Experience: Muri Lagoon, Cook Islands. In CAUTHE 2017: Time for Big Ideas? Re-thinking the Field For Tomorrow; Lee, C., Filep, S., Albrecht, J. N., Coetzee, W. JL, Eds., Department of Tourism, University of Otago: Dunedin, 2017; pp 582–587.

Abstract Both anthropogenic actions and abiotic parameters, such as rainfall, temperature and photoperiod, can affect fruit and flower availability for animals, which consequently affects nutritional status and thus animals’ health. Herein, we investigated whether abiotic factors are related to changes in fruit availability that can lead to changes in feeding behavior and, consequently, in endoparasite load and general health status in two groups of golden-headed lion tamarins (Leontopithecus chrysomelas) living in degraded fragments of Atlantic forest in Southern Bahia, Brazil. We detected that there was a high variation in availability of ripe fruits throughout the year, with lower availability occurring at the end of spring and beginning of summer. Despite this, there was no difference in tamarins’ general health status, body mass and blood counts between seasons. This is probably because during native fruit scarcity, the tamarins eat cultivated species, such as banana (Musa spp.) and jackfruit (Artocarpus heterophyllus). Temperature and daylength were negatively correlated with golden-headed lion tamarin endoparasite loads. Contrary to our expectations, endoparasite loads are not linked to fruit scarcity and consequent changes in feeding behavior. Nevertheless, we found higher parasite diversity in the group of golden-headed lion tamarins that occupied the smallest home range. The smaller the area available, the greater the contact with parasites the animal will have, as they are forced to travel constantly along the same routes in the forest, increasing infection risk and re-infection rates. Our results highlight how animals’ health is associated with environmental health as well as the need for constant monitoring to ensure the effective conservation of endangered species, such as the golden-headed lion tamarin.

AbstractA new algorithm for the quantification of uncertainty in thermal conductivity measurements on polymers according to the Haakvort method is presented. This fast and convenient method using differential scanning calorimetry has been established as DIN EN ISO Standard 11357–8 with an error margin of 5–10%, which is a rather large value when considering that this is an important material parameter for many applications and is often used in combined quantities, such as thermal diffusivity or thermal effusivity. Unfortunately, the DIN EN ISO standard does not provide useful information on the dependence of the error range on the number of specimens or important parameters, such as the height of the specimens or their real contact area. Applying a rigorous statistical approach, based on the law of large numbers (LLN) and different techniques which are also used in well-known methods, such as Monte-Carlo- or Markov chain Monte Carlo (MCMC) algorithms, we establish and investigate a method to optimize the experimental effort to a specific target, especially the number of specimens, the aspect ratio and the real contact surface of the specimen.

Purpose: To determine the effect of pupil dilation on different biometric measurements of eye in high myopes. Study Design: Descriptive observational study. Place and Duration of Study: The study was carried out eye out-patient department of Mayo Hospital, Lahore from August 2018 to December 2018. Methods: We recruited 72 (144 eyes) young, non-cataractous myopes of -6 diopters or more (mean spherical equivalent -7.41 ± 1.35) and age above 15 years (range 15 – 38 years). Sampling was based on non-probability convenient sampling whereas those with any ocular pathology and history of surgery were excluded. Biometric measurements were taken with non-contact biometer Len Star (LS900) with Haigis formula used for IOL power calculation, before and after pupil dilation Data was analyzed on SPSS (v25) and P < .001 was taken as significant. Results: Mean axial length (AL) difference between pre- and post-dilation was -0.006 ± 0.17 (p = 0.66), mean central corneal thickness (CCT) difference was -1.70 ± 8.95 (p = 0.024), mean anterior chamber depth (ACD) difference was -0.018 ± 0.04 (p = 0.001), mean lens thickness (LT) difference was 0.001 ± 0.53 (p = 0.81), mean Keratometry difference was 0.0013 ± 0.14 (p = 0.91), mean white to white (WTW) difference was 0.003 ± 0.01(p = 0.67) and mean IOL power difference was 0.000 ± 0.22 (p = 1.00). Bland Altman plots were drawn to indicate the 95% limits of agreement between pre- and post-dilation measurements. Conclusion: There was a statistically significant difference between pre and post dilation measurements of ACD but no clinically significant change was noted in IOL power calculations indicating that pupil dilation does not affect the biometric measurements. Key Words: Biometry, Len Star, Myopia, Axial length, Anterior chamber depth.

Farm mechanization is the main indicator of modernizing the agriculture and use of farm machines that can take the place of human and animal power in agricultural processes. During field operation the weight of farm machinery compact the agricultural soil due to the contact with the tires or tracks of tractors. The aim of the article is to study the effects of soil texture and moisture content on soil compaction. During field experimental test 5 different depth (5, 10, 15, 20 and 25 cm) were selected for taking soil compaction data and soil samples at three depth range (0-10, 10-20 and 20-30 cm) from Kulumsa Agricultural research center farm land at two seasons. During compaction test 15 sample point data were taken from 0.6 ha farm land. The result of the study shows from average of all sample point the maximum and minimum value of soil compaction value where 3.73 Mpa and 3.032 Mpa during harvesting season and 1.37 Mpa and 1.19 Mpa during seeding season respectively. The laboratory result shows farm land soil is clay with 25.0% of sand, 51% of clay and 24 % silt during both experimental seasons. The maximum and minimum percentage of soil moisture value where 28.92% and 20.04% during harvesting season and 41.8% and 30.8% at 20 to 30 cm and 0 to 10 cm depth respectively.

Affect theory is generally associated with the lifetime’s work of Silvan S. Tomkins, whose four volume work, Affect, Imagery, Consciousness, was published between 1962-92. The volumes argue that humans are subject to a range of innate affects: two positive (interest/excitement; enjoyment/joy), one neutral (surprise/startle) and six negative (distress/anguish; fear/terror; anger/rage; shame/humiliation; dissmell [reaction to a bad smell]; disgust). In a crude “advanced search” using Google, affect is related to emotion in 3,620,000 Web references; to intellect in 1,530,000 instances; and to both intellect and emotion in 1,670,000 cases (Google). Affect may consequently be constructed as a common but complicated response which cannot be simply elided with either emotion or intellect but which involves the integration of both. In particular, affect is generally constructed as a human response to a precipitating stimulus (be it an idea, a physical event, etc). If this is accepted, then Tomkins’s Affect theory might imply that the innate affects only reach conscious awareness as a result of a change in circumstance (e.g., idea or event) which requires a response. The importance of affect as a motivator for action has long been put to good use by advertising and marketing professionals who recognised early in their professions’ development that it is the ESP (emotional selling proposition) that delivers more punch, more quickly, than rational argument. An organisation’s (or individual’s) unique selling point can be rational or emotional, but it is easier for many people marketing a product or service to craft a perceived (unique) difference using emotion rather than logical rationality. For example, Coke and Pepsi are generally constructed as fighting their turf wars based on their emotional appeals, rather than any logical difference between the brands. This paper deals with the use of affect to craft an online therapeutic Website (HeartNET) as a joint ARC-Linkage research project between the National Heart Foundation of Australia (WA Division) and Edith Cowan University’s School of Communications and Multimedia. The research originally started with the idea that heart patients would appreciate the opportunity to communicate online with people going through similar experiences, and that this might create a virtual community of mutually supportive recovering participants. The reality held a few surprises along the way, as we discuss below. HeartNET has been designed to: 1) reduce the disadvantage experienced by people in regional and remote areas; 2) aid the secondary prevention of heart disease in Australia; and, 3) investigate whether increased interaction with an organisation-sponsored affective environment (e.g., the Website) impacts upon perceptions of the organisation. (This might have long-term implications for the financial viability of charitable organisations). In brief, the purpose of the research is to understand the meanings that Web-participants might generate in terms of affective responses to the notion of a shared HeartNET community, and investigate whether these meanings are linked to lifestyle change and responses to the host charity. Ultimately the study aims to determine whether the Website can add value to the participants’ communication and support strategies. The study is still ongoing and has another 18 months to run. Some early results, however, indicate that we need more than a Website and a common life experience to build an affective relationship with others online. The added extra might be what makes the difference between interaction and affective interaction: this needs conscious strategies to generate involvement, aided by the construction of a dynamic (and evolving) Web environment. In short, one stimulus is not enough to generate persistent affective response; the environment has to sustain multiple, evolving and complex stimuli. Online support groups are proliferating because they are satisfying unmet needs and offering an alternative to face-to-face support programs (Madara). Social support also combines some elements of affective community, namely belongingness, intimacy and reciprocity. These community elements can be observed through three levels or layers of social support: 1) belongingness or a sense of integration, 2) bonding which is somewhat more personal and involves linkages between people, and, 3) binding whereby a sense of responsibility for others is experienced and expressed (Lin). Here, social support may prompt an affective response and provide a useful measure of community because it incorporates other elements. Initial Design The project was initially designed to build “an affective interactive space” in the belief that an effective online community might develop thereafter. However, the first stumbling block came in terms of recruiting participants: this took almost nine-months longer than anticipated (even once Ethics approval had been granted). Partly this was due to a specific focus on recruiting people born between 1946–64 (“baby boomers”), partly it was due to the requirement that participants had access to the Web, and partly it was because we sought to specifically recruit non-metropolitan Western Australians who had suffered a health-challenging heart-related episode. We were hoping to identify at least 80 such people, to allow for a control group in addition to the people invited to join the online community. Stage 1 was to be the analysis of the functioning of the online community; Stage 2 would take the form of interviews of both community members and the control group. One aspect of the research was to determine whether online participants perceived themselves as belonging to an online community (as opposed to “interacting on a Website”) and whether this community was constructed as therapeutic, or in other ways beneficial. Once the requisite number of people had been recruited, the Website went “live”. Usage was extremely hesitant, and this was the case even though more people were added to the Website than originally planned. (In the end we had to rely upon the help of cardiologists publicising the research among their heart patients. This had a continuing trickle effect that meant that the Website ultimately had 68 people who agreed to participate, of whom 15 never logged in. Of the remaining 53 participants, 31 logged in but never posted anything. Of the 22 people who posted, 17 made between one and four contributions. The remaining five people posted five or more times, and included the researcher and an experienced facilitator, Sven (name changed), who was serving in a “professionally-supportive” role (as well as a recovering heart patient himself). This was hardly the vibrant, affectively-supportive environment for which we had been planning. Even with the key researcher-moderator calling people individually and talking them through the mechanics of how to post, the interactions fell away and eventually ceased, more or less, altogether after 11 weeks. One of the particularly distressing implications of the lack of interaction was the degree of self-revelation that some participants had offered when first logging onto the site. New members, for example, were encouraged to “share their heart story”. Susan’s (name changed) is an example of how open these could be: I had a heart attack in February 2004. This came as a huge shock. I didn’t have any of the usual risk factors. Although my father has Coronary Vascular Disease, he didn’t have any symptoms until his mid 60s and never had a heart attack. I had angioplasty and a stent. I accept I will be taking medication for the rest of my life. I’m fine physically but am having treatment for depression, which was diagnosed 6 months after my heart attack. In normal social situations an affective revelation such as “I’m fine physically but am having treatment for depression” would elicit a sympathetic response. In fact, such “stories” did often get responses from active members (and always got a response from the researcher-moderator), but the original poster would often not log in again and would thus not receive the group’s feedback. In this case, it was particularly relevant that the poster should have learned that other site users were aware that some heart medication has depression as a common side effect and were urging Susan to ask her doctor whether this could be a factor in her case. A further problem was that there was no visible traffic on much of the Website. During the first 12 weeks, only seven of 155 posts were made to the discussion forums. Instead, participants tended to leave individual messages for each other in “private spaces” that had been designed as blogs, to allow people to keep online diaries (and where blog-visitors had the opportunity to post comments, feedback and encouragement). It was speculated that this pattern of invisible interaction was symptomatic of a generation that felt most comfortable with using the internet for e-mail, and were unfamiliar with discussion boards. (Privacy, ethics, research design and good practice meant that the only way that participants could contact each other was via the Website; they couldn’t use a private e-mail address.) The absence of visible interactive feedback was a disincentive to participation for even the most active posters and it was clear that, while some people felt able to reveal aspects of themselves and their heart condition online, they needed more that this opportunity to encourage them to return and participate further. Effectively, the research was in crisis. Crisis Measures After 10 weeks of the HeartNET interaction stalling, and then crashing, it was decided to do four things: write up what had been learned about what didn’t work (before the site was “polluted” by what we hoped would be the solution); redesign the Website to allow more ways to interact privately as well as publicly; throw it open to anyone who wished to join so that there was a more dynamic, developing momentum; use a “newbie” icon to indicate new network members joining in the previous seven days so that these people could be welcomed by existing members (who would also have an incentive to log in at least weekly). Five weeks into the revamped Website a number of things have become apparent. There is some “incidental traffic” apart from research-recruited participants and word-of-mouth, for example (Jane): “I discovered this site while surfing the net. I haven’t really sought much support since my heart attack which was nearly a year ago, but wish I had since it would have made those darker days a lot easier to get through.” An American heart patient has joined the community (Sam): “I have a lot to be positive about and feel grateful to have found this site full of caring people.” Further, some returnees, who had experienced the first iteration of the site, were warm with acknowledgement (Betty): “the site is taking off in leeps [sic] and bounds. You should all be so proud.” People are making consecutive postings, updating and developing their stories, revealing their need for support and recognising the help when they receive it. It is not hard to empathise with “Wonky” (name changed) who may not have family in whom s/he can confide: (Wonky, post 12, Wed) [I need] preventative surgery of this aorta [addressing a bi-cuspid aortic valve] before it has an aneurysm or dissects … and YES I AM SCARED … but trying to be brave cos at least now I know what is wrong with me and its kinda fixable … After being asked by interested members to update the community on his/her progress, Wonky makes the following posts: (Wonky, post 13, Wed) […] I am currently petrified … And anxiously waiting to see the cardio at 3 pm Thursday regarding the results of my aorta echo … and when they are going to decide I need lifesaving surgery … (Wonky, post 15, Fri) ok…so I am up to Friday morning and fasting for the CT scan of the dodgy aorta etc … this morning … why do I get hungry when I have to fast yet any other day I really have to force myself to remember to even eat … (Sven, online support person, Fri) great news [Wonky] and I sense a more ‘coming to terms’ understanding of your situation on your part. You’re in good hands believe you me and you are surrounded by a great number of friends who are here to cheer you on. Keep smiling. […] (Wonky, post 16, Sun) Yes [Sven], you are exactly right […] [declining health] I guess is what scared me and plus I had pretty-much not bothered to research into the condition early on when I was first diagnosed … but yeah … my cardio guy is wonderful and has assured me I am not going to drop dead any-time soon from this … For people who had experienced heart disease without support, the value of the HeartNET site was self-evident (Jace): “My heart attack was 18 months ago and I knew no one with a similar experience. My family and friends were very supportive but they were as shocked as me. Heartnet has given me the opportunity to hear other people’s stories.” Almost two weeks later, Jace was able to offer the benefit of her experience to someone suffering from panic attacks: I had several panic attacks post my heart attack. They are very frightening aren’t they? They seemed to come out of nowhere and I felt very out of control. I found making myself breath[e] more slowly and deeply, while telling myself to calm down, helped a lot. I also started listening to relaxation CDs as well. Take care, [Jace]. Others have asked for advice: (Anne): “Everyone, and I mean everyone, has been saying ‘are you sure you want to go [back to work]?’ Does anyone have coping strategies for those well meaning colleagues and bosses who think you need to be wrapped up in cotton wool?” Several people have taken the opportunity to confide their deepest fear: (Marc): “Why me? Why now? Can I get back to work normally? Every twinge you feel, you think is the big one or another attack that will get you this time.” (Anne): “I decided to spend last night in A&E [accident and emergency] after a nice little ambulance ride. It turned out to be nothing more than stress and indigestion but it scared the crap out of me. I have taken it so easy today and intend to rest up from now on in.” Some of the posts are both celebratory and inspirational (although the one cited below required a rider to the effect that any change in activity should be checked with a GP or specialist): (Joggy) I mentioned on an earlier post that I was going to run the 4km in the City to Surf and I actually did it. This is from someone who has probably run no more than 100 metres in one go in her life and guess what, I quite like it now […] I know that I am way fitter now than I have ever been and in a nutshell it’s great. Others see support as a two-way street: (Drew) “If you no longer fell [sic] YOU need the support, keep in mind others may benefit from YOUR support.” Discussion Tomkins’s Affect theory suggests that humans are subject to two positive affects: interest/excitement; enjoyment/joy, and one neutral affect: surprise/startle, along with six negative affects. All these affects are decoded/interpreted from facial expressions and require face-to-face interactions to be fully perceived. When we look at what affective prompts may be inciting people to log into HeartNET and communicate online, however, it becomes hard to second guess the affective motivation. Interest/excitement may be overstating the emotional impulse while enjoyment/joy may be an extreme way to describe the pleasure of recognition and identification with others in a similar situation. Arguably, HeartNET offers an opportunity to minimise negative affect, in particular “distress/anguish; fear/terror; anger/rage; shame/humiliation” – all of which may be present in some people’s experiences of heart disease. A strategy for reducing negative affect may be as valuable as the promise of increasing the experience of positive affect. As for the rational or emotional impact, it seems clear from the first stages of the research that rationally people were willing to take part in the trial and agreed to participate, but a large majority then failed to either log in or post any contribution. The site came to emotional life only when it was less obviously a “research project” (in the sense that all participants still had to log in via an ethics disclosure and informed consent screen) in that people could join when and if they were motivated to do so, and were invited to participate by those who were already online. Since the Website was revamped and relaunched on 2 August 2005 a further 124 people have joined. It appears that HeartNET is now both an affective and effective success. References “Affective Therapy.” Affective Therapy Website: Tomkins and Affect. 9 Oct. 2005 http://www.affectivetherapy.co.uk/Tomkins_Affect.htm>. “Google Advanced Search.” Google. 1 Nov. 2005 http://www.google.com.au/advanced_search>. Lin, Nan. Conceptualizing Social Support: Social Support, Life Events, and Depression. Ed. Nan Lin, Alfred Dean, & Walter Ensel. Orlando: Florida, Academic Press, 1986. Madara, Edward. “The Mutual-Aid Self-Help Online Revolution”. Social Policy 27 (1997): 20. Tomkins, Silvan S. Affect, Imagery, Consciousness (Volume 1): The Positive Affects. New York: Springer, 1962. ———. Affect, Imagery, Consciousness (Volume 2): The Negative Affects. New York: Springer, 1963. ———. Affect, Imagery, Consciousness (Volume 3): The Negative Affects: Anger and Fear. New York: Springer, 1991. ———. Affect, Imagery, Consciousness (Volume 4): Cognition: Duplication and Transformation of Information. New York: Springer, 1992. Citation reference for this article MLA Style Bonniface, Leesa, Lelia Green, and Maurice Swanson. "Affect and an Effective Online Therapeutic Community." M/C Journal 8.6 (2005). echo date('d M. Y'); ?> <http://journal.media-culture.org.au/0512/05-bonnifacegreenswanson.php>. APA Style Bonniface, L., L. Green, and M. Swanson. (Dec. 2005) "Affect and an Effective Online Therapeutic Community," M/C Journal, 8(6). Retrieved echo date('d M. Y'); ?> from <http://journal.media-culture.org.au/0512/05-bonnifacegreenswanson.php>.

Introduction: Brutal?The word “brutal” has associations with cruelty, inhumanity, and aggression. Within the field of architecture, however, the term “Brutalism” refers to a post-World War II Modernist style, deriving from the French phrase betón brut, which means raw concrete (Clement 18). Core traits of Brutalism include functionalist design, daring geometry, overbearing scale, and the blatant exposure of structural materials, chiefly concrete and steel (Meades 1).The emergence of Brutalism coincided with chronic housing shortages in European countries ravaged by World War II (Power 5) and government-sponsored slum clearance in the UK (Power 190; Baker). Brutalism’s promise to accommodate an astonishing number of civilians within a minimal area through high-rise configurations and elevated walkways was alluring to architects and city planners (High Rise Dreams). Concrete was the material of choice due to its affordability, durability, and versatility; it also allowed buildings to be erected quickly (Allen and Iano 622).The Brutalist style was used for cultural centres, such as the Perth Concert Hall in Western Australia, educational institutions such as the Yale School of Architecture, and government buildings such as the Secretariat Building in Chandigarh, India. However, as pioneering Brutalist architect Alison Smithson explained, the style achieved full expression by “thinking on a much bigger scale somehow than if you only got [sic] one house to do” (Smithson and Smithson, Conversation 40). Brutalism, therefore, lent itself to the design of large residential complexes. It was consequently used worldwide for public housing developments, that is, residences built by a government authority with the aim of providing affordable housing. Notable examples include the Western City Gate in Belgrade, Serbia, and Habitat 67 in Montreal, Canada.Brutalist architecture polarised opinion and continues to do so to this day. On the one hand, protected cultural heritage status has been awarded to some Brutalist buildings (Carter; Glancey) and the style remains extremely influential, for example in the recent award-winning work of architect Zaha Hadid (Niesewand). On the other hand, the public housing projects associated with Brutalism are widely perceived as failures (The Great British Housing Disaster). Many Brutalist objects currently at risk of demolition are social housing estates, such as the Smithsons’ Robin Hood Gardens in London, UK. Whether the blame for the demise of such housing developments lies with architects, inhabitants, or local government has been widely debated. In the UK and USA, local authorities had relocated families of predominantly lower socio-economic status into the newly completed developments, but were unable or unwilling to finance subsequent maintenance and security costs (Hanley 115; R. Carroll; The Pruitt-Igoe Myth). Consequently, the residents became fearful of criminal activity in staircases and corridors that lacked “defensible space” (Newman 9), which undermined a vision of “streets in the sky” (Moran 615).In spite of its later problems, Brutalism’s architects had intended to develop a style that expressed 1950s contemporary living in an authentic manner. To them, this meant exposing building materials in their “raw” state and creating an aesthetic for an age of science, machine mass production, and consumerism (Stadler 264; 267; Smithson and Smithson, But Today 44). Corporeal sensations did not feature in this “machine” aesthetic (Dalrymple). Exceptionally, acclaimed Brutalist architect Ernö Goldfinger discussed how “visual sensation,” “sound and touch with smell,” and “the physical touch of the walls of a narrow passage” contributed to “sensations of space” within architecture (Goldfinger 48). However, the effects of residing within Brutalist objects may not have quite conformed to predictions, since Goldfinger moved out of his Brutalist construction, Balfron Tower, after two months, to live in a terraced house (Hanley 112).An abstract perspective that favours theorisation over subjective experiences characterises discourse on Brutalist social housing developments to this day (Singh). There are limited data on the everyday lived experience of residents of Brutalist social housing estates, both then and now (for exceptions, see Hanley; The Pruitt-Igoe Myth; Cooper et al.).Yet, our bodily interaction with the objects around us shapes our lived experience. On a broader physical scale, this includes the structures within which we live and work. The importance of the interaction between architecture and embodied being is increasingly recognised. Today, architecture is described in corporeal terms—for example, as a “skin” that surrounds and protects its human inhabitants (Manan and Smith 37; Armstrong 77). Biological processes are also inspiring new architectural approaches, such as synthetic building materials with life-like biochemical properties (Armstrong 79), and structures that exhibit emergent behaviour in response to human presence, like a living system (Biloria 76).In this article, we employ an autoethnographic perspective to explore the corporeal effects of Brutalist buildings, thereby revealing a new dimension to the anthropological significance of these controversial structures. We trace how they shape the physicality of the bodies interacting within them. Our approach is one step towards considering the historically under-appreciated subjective, corporeal experience elicited in interaction with Brutalist objects.Method: An Autoethnographic ApproachAutoethnography is a form of self-narrative research that connects the researcher’s personal experience to wider cultural understandings (Ellis 31; Johnson). It can be analytical (Anderson 374) or emotionally evocative (Denzin 426).We investigated two Brutalist residential estates in London, UK:(i) The Barbican Estate: This was devised to redevelop London’s severely bombed post-WWII Cripplegate area, combining private residences for middle class professionals with an assortment of amenities including a concert hall, library, conservatory, and school. It was designed by architects Chamberlin, Powell, and Bon. Opened in 1982, the Estate polarised opinion on its aesthetic qualities but has enjoyed success with residents and visitors. The development now comprises extremely expensive housing (Brophy). It was Grade II-listed in 2001 (Glancey), indicating a status of architectural preservation that restricts alterations to significant buildings.(ii) Trellick Tower: This was built to replace dilapidated 19th-century housing in the North Kensington area. It was designed by Hungarian-born architect Ernő Goldfinger to be a social housing development and was completed in 1972. During the 1980s and 1990s, it became known as the “Tower of Terror” due to its high level of crime (Hanley 113). Nevertheless, Trellick Tower was granted Grade II listed status in 1998 (Carter), and subsequent improvements have increased its desirability as a residence (R. Carroll).We explored the grounds, communal spaces, and one dwelling within each structure, independently recording our corporeal impressions and sensations in detailed notes, which formed the basis of longhand journals written afterwards. Our analysis was developed through co-constructed autoethnographic reflection (emerald and Carpenter 748).For reasons of space, one full journal entry is presented for each Brutalist structure, with an excerpt from each remaining journal presented in the subsequent analysis. To identify quotations from our journals, we use the codes R- and N- to refer to RB’s and NC’s journals, respectively; we use -B and -T to refer to the Barbican Estate and Trellick Tower, respectively.The Barbican Estate: Autoethnographic JournalAn intricate concrete world emerges almost without warning from the throng of glass office blocks and commercial buildings that make up the City of London's Square Mile. The Barbican Estate comprises a multitude of low-rise buildings, a glass conservatory, and three enormous high-rise towers. Each modular building component is finished in the same coarse concrete with burnished brick underfoot, whilst the entire structure is elevated above ground level by enormous concrete stilts. Plants hang from residential balconies over glimmering pools in a manner evocative of concrete Hanging Gardens of Babylon.Figure 1. Barbican Estate Figure 2. Cromwell Tower from below, Barbican Estate. Figure 3: The stairwell, Cromwell Tower, Barbican Estate. Figure 4. Lift button pods, Cromwell Tower, Barbican Estate.R’s journalMy first footsteps upon the Barbican Estate are elevated two storeys above the street below, and already an eerie calm settles on me. The noise of traffic and the bustle of pedestrians have seemingly been left far behind, and a path of polished brown brick has replaced the paving slabs of the city's pavement. I am made more aware of the sound of my shoes upon the ground as I take each step through the serenity.Running my hands along the walkway's concrete sides as we proceed further into the estate I feel its coarseness, and look up to imagine the same sensation touching the uppermost balcony of the towers. As we travel, the cold nature and relentless employ of concrete takes over and quickly becomes the norm.Our route takes us through the Barbican's central Arts building and into the Conservatory, a space full of plant-life and water features. The noise of rushing water comes as a shock, and I'm reminded just how hauntingly peaceful the atmosphere of the outside estate has been. As we leave the conservatory, the hush returns and we follow another walkway, this time allowing a balcony-like view over the edge of the estate. I'm quickly absorbed by a sensation I can liken only to peering down at the ground from a concrete cloud as we observe the pedestrians and traffic below.Turning back, we follow the walkways and begin our approach to Cromwell Tower, a jagged structure scraping the sky ahead of us and growing menacingly larger with every step. The estate has up till now seemed devoid of wind, but even so a cold begins to prickle my neck and I increase my speed toward the door.A high-ceilinged foyer greets us as we enter and continue to the lifts. As we push the button and wait, I am suddenly aware that carpet has replaced bricks beneath my feet. A homely sensation spreads, my breathing slows, and for a brief moment I begin to relax.We travel at heart-racing speed upwards to the 32nd floor to observe the view from the Tower's fire escape stairwell. A brief glance over the stair's railing as we enter reveals over 30 storeys of stair casing in a hard-edged, triangular configuration. My mind reels, I take a second glance and fail once again to achieve focus on the speck of ground at the bottom far below. After appreciating the eastward view from the adjacent window that encompasses almost the entirety of Central London, we make our way to a 23rd floor apartment.Entering the dwelling, we explore from room to room before reaching the balcony of the apartment's main living space. Looking sheepishly from the ledge, nothing short of a genuine concrete fortress stretches out beneath us in all directions. The spirit and commotion of London as I know it seems yet more distant as we gaze at the now miniaturized buildings. An impression of self-satisfied confidence dawns on me. The fortress where we stand offers security, elevation, sanctuary and I'm furnished with the power to view London's chaos at such a distance that it's almost silent.As we leave the apartment, I am shadowed by the same inherent air of tranquillity, pressing yet another futuristic lift access button, plummeting silently back towards the ground, and padding across the foyer's soft carpet to pursue our exit route through the estate's sky-suspended walkways, back to the bustle of regular London civilization.Trellick Tower: Autoethnographic JournalThe concrete majesty of Trellick Tower is visible from Westbourne Park, the nearest Tube station. The Tower dominates the skyline, soaring above its neighbouring estate, cafes, and shops. As one nears the Tower, the south face becomes visible, revealing the suspended corridors that join the service tower to the main body of flats. Light of all shades and colours pours from its tightly stacked dwellings, which stretch up into the sky. Figure 5. Trellick Tower, South face. Figure 6. Balcony in a 27th-floor flat, Trellick Tower.N’s journalOutside the tower, I sense danger and experience a heightened sense of awareness. A thorny frame of metal poles holds up the tower’s facade, each pole poised as if to slip down and impale me as I enter the building.At first, the tower is too big for comprehension; the scale is unnatural, gigantic. I feel small and quite squashable in comparison. Swathes of unmarked concrete surround the tower, walls that are just too high to see over. Who or what are they hiding? I feel uncertain about what is around me.It takes some time to reach the 27th floor, even though the lift only stops on every 3rd floor. I feel the forces of acceleration exert their pressure on me as we rise. The lift is very quiet.Looking through the windows on the 27th-floor walkway that connects the lift tower to the main building, I realise how high up I am. I can see fog. The city moves and modulates beneath me. It is so far away, and I can’t reach it. I’m suspended, isolated, cut off in the air, as if floating in space.The buildings underneath appear tiny in comparison to me, but I know I’m tiny compared to this building. It’s a dichotomy, an internal tension, and feels quite unreal.The sound of the wind in the corridors is a constant whine.In the flat, the large kitchen window above the sink opens directly onto the narrow, low-ceilinged corridor, on the other side of which, through a second window, I again see London far beneath. People pass by here to reach their front doors, moving so close to the kitchen window that you could touch them while you’re washing up, if it weren’t for the glass. Eye contact is possible with a neighbour, or a stranger. I am close to that which I’m normally separated from, but at the same time I’m far from what I could normally access.On the balcony, I have a strong sensation of vertigo. We are so high up that we cannot be seen by the city and we cannot see others. I feel physically cut off from the world and realise that I’m dependent on the lift or endlessly spiralling stairs to reach it again.Materials: sharp edges, rough concrete, is abrasive to my skin, not warm or welcoming. Sharp little stones are embedded in some places. I mind not to brush close against them.Behind the tower is a mysterious dark maze of sharp turns that I can’t see around, and dark, narrow walkways that confine me to straight movements on sloping ramps.“Relentless Employ of Concrete:” Body versus Stone and HeightThe “relentless employ of concrete” (R-B) in the Barbican Estate and Trellick Tower determined our physical interactions with these Brutalist objects. Our attention was first directed towards texture: rough, abrasive, sharp, frictive. Raw concrete’s potential to damage skin, should one fall or brush too hard against it, made our bodies vulnerable. Simultaneously, the ubiquitous grey colour and the constant cold anaesthetised our senses.As we continued to explore, the constant presence of concrete, metal gratings, wire, and reinforced glass affected our real and imagined corporeal potentialities. Bodies are powerless against these materials, such that, in these buildings, you can only go where you are allowed to go by design, and there are no other options.Conversely, the strength of concrete also has a corporeal manifestation through a sense of increased physical security. To R, standing within the “concrete fortress” of the Barbican Estate, the object offered “security, elevation, sanctuary,” and even “power” (R-B).The heights of the Barbican’s towers (123 metres) and Trellick Tower (93 metres) were physically overwhelming when first encountered. We both felt that these menacing, jagged towers dominated our bodies.Excerpt from R’s journal (Trellick Tower)Gaining access to the apartment, we begin to explore from room to room. As we proceed through to the main living area we spot the balcony and I am suddenly aware that, in a short space of time, I had abandoned the knowledge that some 26 floors lay below me. My balance is again shaken and I dig my heels into the laminate flooring, as if to achieve some imaginary extra purchase.What are the consequences of extreme height on the body? Certainly, there is the possibility of a lethal fall and those with vertigo or who fear heights would feel uncomfortable. We discovered that height also affects physical instantiation in many other ways, both empowering and destabilising.Distance from ground-level bustle contributed to a profound silence and sense of calm. Areas of intermediate height, such as elevated communal walkways, enhanced our sensory abilities by granting the advantage of observation from above.Extreme heights, however, limited our ability to sense the outside world, placing objects beyond our range of visual focus, and setting up a “bizarre segregation” (R-T) between our physical presence and that of the rest of the world. Height also limited potentialities of movement: no longer self-sufficient, we depended on a working lift to regain access to the ground and the rest of the city. In the lift itself, our bodies passively endured a cycle of opposing forces as we plummeted up or down numerous storeys in mere seconds.At both locations, N noticed how extreme height altered her relative body size: for example, “London looks really small. I have become huge compared to the tiny city” (N-B). As such, the building’s lift could be likened to a cake or potion from Lewis Carroll’s Alice in Wonderland. This illustrates how the heuristics that we use to discern visual perspective and object size, which are determined by the environment in which we live (Segall et al.), can be undermined by the unusual scales and distances found in Brutalist structures.Excerpt from N’s journal (Barbican Estate)Warning: These buildings give you AFTER-EFFECTS. On the way home, the size of other buildings seems tiny, perspectives feel strange; all the scales seem to have been re-scaled. I had to become re-used to the sensation of travelling on public trains, after travelling in the tower lifts.We both experienced perceptual after-effects from the disproportional perspectives of Brutalist spaces. Brutalist structures thus have the power to affect physical sensations even when the body is no longer in direct interaction with them!“Challenge to Privacy:” Intersubjective Ideals in Brutalist DesignAs embodied beings, our corporeal manifestations are the primary transducers of our interactions with other people, who in turn contribute to our own body schema construction (Joas). Architects of Brutalist habitats aimed to create residential utopias, but we found that the impact of their designs on intersubjective corporeality were often incoherent and contradictory. Brutalist structures positioned us at two extremes in relation to the bodies of others, forcing either an uncomfortable intersection of personal space or, conversely, excessive separation.The confined spaces of the lifts, and ubiquitous narrow, low-ceilinged corridors produced uncomfortable overlaps in the personal space of the individuals present. We were fascinated by the design of the flat in Trellick Tower, where the large kitchen window opened out directly onto the narrow 27th-floor corridor, as described in N’s journal. This enforced a physical “challenge to privacy” (R-T), although the original aim may have been to promote a sense of community in the “streets in the sky” (Moran 615). The inter-slotting of hundreds of flats in Trellick Tower led to “a multitude of different cooking aromas from neighbouring flats” (R-T) and hence a direct sensing of the closeness of other people’s corporeal activities, such as eating.By contrast, enormous heights and scales constantly placed other people out of sight, out of hearing, and out of reach. Sharp-angled walkways and blind alleys rendered other bodies invisible even when they were near. In the Barbican Estate, huge concrete columns, behind which one could hide, instilled a sense of unease.We also considered the intersubjective interaction between the Brutalist architect-designer and the inhabitant. The elements of futuristic design—such as the “spaceship”-like pods for lift buttons in Cromwell Tower (N-B)—reconstruct the inhabitant’s physicality as alien relative to the Brutalist building, and by extension, to the city that commissioned it.ReflectionsThe strength of the autoethnographic approach is also its limitation (Chang 54); it is an individual’s subjective perspective, and as such we cannot experience or represent the full range of corporeal effects of Brutalist designs. Corporeal experience is informed by myriad factors, including age, body size, and ability or disability. Since we only visited these structures, rather than lived in them, we could have experienced heightened sensations that would become normalised through familiarity over time. Class dynamics, including previous residences and, importantly, the amount of choice that one has over where one lives, would also affect this experience. For a full perspective, further data on the everyday lived experiences of residents from a range of different backgrounds are necessary.R’s reflectionDespite researching Brutalist architecture for years, I was unprepared for the true corporeal experience of exploring these buildings. Reading back through my journals, I'm struck by an evident conflict between stylistic admiration and physical uneasiness. I feel I have gained a sympathetic perspective on the notion of residing in the structures day-to-day.Nevertheless, analysing Brutalist objects through a corporeal perspective helped to further our understanding of the experience of living within them in a way that abstract thought could never have done. Our reflections also emphasise the tension between the physical and the psychological, whereby corporeal struggle intertwines with an abstract, aesthetic admiration of the Brutalist objects.N’s reflectionIt was a wonderful experience to explore these extraordinary buildings with an inward focus on my own physical sensations and an outward focus on my body’s interaction with others. On re-reading my journals, I was surprised by the negativity that pervaded my descriptions. How does physical discomfort and alienation translate into cognitive pleasure, or delight?ConclusionBrutalist objects shape corporeality in fundamental and sometimes contradictory ways. The range of visual and somatosensory experiences is narrowed by the ubiquitous use of raw concrete and metal. Materials that damage skin combine with lethal heights to emphasise corporeal vulnerability. The body’s movements and sensations of the external world are alternately limited or extended by extreme heights and scales, which also dominate the human frame and undermine normal heuristics of perception. Simultaneously, the structures endow a sense of physical stability, security, and even power. By positioning multiple corporealities in extremes of overlap or segregation, Brutalist objects constitute a unique challenge to both physical privacy and intersubjective potentiality.Recognising these effects on embodied being enhances our current understanding of the impact of Brutalist residences on corporeal sensation. This can inform the future design of residential estates. Our autoethnographic findings are also in line with the suggestion that Brutalist structures can be “appreciated as challenging, enlivening environments” exactly because they demand “physical and perceptual exertion” (Sroat). 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Introduction I am on the phone with a journalist discussing my research into anti-vaccination. As the conversation winds up, they ask a question I have come to expect: "how big do you think this is?" My answer is usually some version of the following: that we have no way of knowing. I and my fellow researchers can only see the information that is public or in the sunlight. How anti-vaccination information spreads through private networks is dark to us. It is private and necessarily so. This means that we cannot track how these conversations spread in the private or parochial spaces of Facebook, nor can we consider how they might extend into other modes of mediated communication. Modern communication is a complex and multiplatform accomplishment. Consider this: I am texting with my friend, I send her a selfie, in the same moment I hear a notification, she has DMed me a relevant Instagram post via that app. I move to Instagram and share another post in response; we continue our text message conversation there. Later in the day, I message her on Facebook Messenger while participating in a mutual WhatsApp group chat. The next day we Skype, and while we talk, we send links back and forth, which in hindsight are as clear as hieroglyphics before the Rosetta stone. I comment on her Twitter post, and we publicly converse back and forth briefly while other people like our posts. None of these instances are discrete conversational events, even though they occur on different platforms. They are iterations on the same themes, and the archival properties of social media and private messaging apps mean that neither of you forgets where you left off. The conversation slides not only between platforms and contexts but in and out of visibility. Digitally mediated conversation hums in the background of daily life (boring meetings, long commutes and bad dates) and expands our understanding of the temporal and sequential limits of conversation. In this article, I will explore digitally-mediated cross-platform conversation as a problem in two parts, and how we can understand it as part of the 'dark social'. Specifically, I want to draw attention to how 'dark' online spaces are part of our everyday communicative practices and are not necessarily synonymous with the illicit, illegal, or deviant. I argue that the private conversations we have online are also part of the dark social web, insofar as they are hidden from the public eye. When I think of dark social spaces, I think of what lies beneath the surface of murky waters, what hides behind in backstage areas, and the moments between platforms. In contrast, 'light' (or public) social spaces are often perceived as siloed. The boundaries between these platforms are artificially clean and do not appear to leak into other spaces. This article explores the dark and shadowed spaces of online conversation and considers how we might approach them as researchers. Conversations occur in the backchannels of social media platforms, in private messaging functions that are necessarily invisible to the researcher's gaze. These spaces are distinct from the social media activity analysed by Marwick and boyd. Their research examining teens' privacy strategies on social media highlights how social media posts that multiple audiences may view often hold encoded meanings. Social media posts are a distinct and separate category of activity from meditated conversations that occur one to one, or in smaller group chat settings. Second is the disjunction between social media platforms. Users spread their activity across any number of social media platforms, according to social and personal logics. However, these movements are difficult to capture; it is difficult to see in the dark. Platforms are not hermeneutically sealed off from each other, or the broader web. I argue that understanding how conversation moves between platforms and in the backstage spaces of platforms are two parts of the same dark social puzzle. Conversation Online Digital media have changed how we maintain our social connections across time and space. Social media environments offer new possibilities for communication and engagement as well as new avenues for control. Calls and texts can be ignored, and our phones are often used as shields. Busying ourselves with them can help us avoid unwanted face-to-face conversations. There are a number of critiques regarding the pressure of always-on contact, and a growing body of research that examines how users negotiate these demands. By examining group messaging, Mannell highlights how the boundaries of these chats are porous and flexible and mark a distinct communicative break from previous forms of mobile messaging, which were largely didactic. The advent of group chats has also led to an increasing complication of conversation boundaries. One group chat may have several strands of conversation sporadically re-engaged with over time. Manell's examination of group chats empirically illustrates the complexity of digitally-mediated conversations as they move across private, parochial, and public spaces in a way that is not necessarily temporally linear. Further research highlights the networked nature of digitally mediated interpersonal communication and how conversations sprawl across multiple platforms (Burchell). Couldry (16, 17) describes this complex web as the media manifold. This concept encompasses the networked platforms that comprise it and refers to its embeddedness in daily life. As we no longer “log on” to the internet to send and receive email, the manifold is both everywhere and nowhere; so too are our conversations. Gershon has described the ways we navigate the communicative affordances of these platforms as “media ideologies" which are the "beliefs, attitudes, and strategies about the media they [individuals] use" (391). Media ideologies also contain implicit assumptions about which platforms are best for delivering which kinds of messages. Similarly, Burchell argues that the relational ordering of available media technologies is "highly idiosyncratic" (418). Burchell contends that this idiosyncratic ordering is interdependent and relational, and that norms about what to do when are both assumed by individuals and learnt in their engagement with others (418). The influence of others allows us to adjust our practices, or as Burchell argues, "to attune and regulate one's own conduct … and facilitate engagement despite the diverse media practices of others" (418). In this model, individuals are constantly learning and renegotiating norms of conversation on a case by case, platform by platform basis. However, I argue that it is more illuminating to consider how we have collectively developed an implicit and unconscious set of norms and signals that govern our (collective) conduct, as digitally mediated conversation has become embedded in our daily lives. This is not to say that everyone has the same conversational skill level, but rather that we have developed a common toolbox for understanding the ebb and flow of digitally mediated conversations across platforms. However, these norms are implicit, and we only have a partial understanding of how they are socially achieved in digitally-mediated conversation. What Lies Beneath Most of what we do online is assumed not to be publicly visible. While companies like Facebook trace us across the web and peer into every nook and cranny of our private use patterns, researchers have remained focussed on what lies above in the light, not below, in the dark. This has meant an overwhelming focus on single platform studies that rely on the massification of data as a default measure for analysing sentiment and behaviour online. Sociologically, we know that what occurs in dark social spaces, or backstage, is just as important to social life as what happens in front of an audience (Goffman). Goffman's research uses the metaphor of the theatre to analyse how social life is accomplished as a performance. He highlights that (darkened) backstage spaces are those where we can relax, drop our front, and reveal parts of our (social) self that may be unpalatable to a broader audience. Simply, the public data accessible to researchers on social media are “trace data”, or “trace conversation”, from the places where conversations briefly leave (public) footprints and can be tracked and traced before vanishing again. Alternatively, we can visualise internet researchers as swabbing door handles for trace evidence, attempting to assemble a narrative out of a left-behind thread or a stray fingerprint. These public utterances, often scraped through API access, are only small parts of the richness of online conversation. Conversations weave across multiple platforms, yet single platforms are focussed on, bracketing off their leaky edges in favour of certainty. We know the social rules of platforms, but less about the rules between platforms, and in their darker spaces. Conversations briefly emerge into the light, only to disappear again. Without understanding how conversation is achieved and how it expands and contracts and weaves in and out of the present, we are only ever guessing about the social dynamics of mediated conversation as they shift between light, dark, and shadow spaces. Small things can cast large shadows; something that looms large may be deceptively small. Online they could be sociality distorted by disinformation campaigns or swarms of social bots. Capturing the Unseen: An Ethnomethodological Approach Not all data are measurable, computable, and controllable. There is uncertainty beyond what computational logics can achieve. Nooks and crannies of sociality exist beyond the purview of computable data. This suggests that we can apply pre-digital social research methods to capture these “below the surface” conversations and understand their logics. Sociologists have long understood that conversation is a social accomplishment. In the 1960s, sociologist Harvey Sacks developed conversation analysis as an ethnomethodological technique that seeks to understand how social life is accomplished in day-to-day conversation and micro-interactions. Conversation analysis is a detailed and systematic account of how naturally-occurring talk is socially ordered, and has been applied across a number of social contexts, including news interviews, judicial settings, suicide prevention hotlines, therapy sessions, as well as regular phone conversations (Kitzinger and Frith). Conversation analysis focusses on fine-grained detail, all of the little patterns of speech that make up a conversation; for example, the pauses, interruptions, self-corrections, false starts, and over-speaking. Often these too are hidden features of conversation, understood implicitly, but hovering on the edges of our social knowledge. One of the most interesting uses of conversational analysis is to understand refusal, that is, how we say 'no' as a social action. This body of research turns common-sense social knowledge – that saying no is socially difficult – into a systemic schema of social action. For instance, acceptance is easy to achieve; saying yes typically happens quickly and without hesitation. Acceptances are not qualified; a straightforward 'yes' is sufficient (Kitzinger and Frith). However, refusals are much more socially complex. Refusal is usually accomplished by apologies, compliments, and other palliative strategies that seek to cushion the blow of refusals. They are delayed and indirect conversational routes, indicating their status as a dispreferred social action, necessitating their accompaniment by excuses or explanations (Kitzinger and Frith). Research by Kitzinger and Frith, examining how women refuse sexual advances, illustrates that we all have a stock of common-sense knowledge about how refusals are typically achieved, which persists across various social contexts, including in our intimate relationships. Conversation analysis shows us how conversation is achieved and how we understand each other. To date, conversation analysis techniques have been applied to spoken conversation but not yet extended into text-based mediated conversation. I argue that we could apply insights from conversation analysis to understand the rules that govern digitally mediated conversation, how conversation moves in the spaces between platforms, and the rules that govern its emergence into public visibility. What rules shape the success of mediated communication? How can we understand it as a social achievement? When conversation analysis walks into the dark room it can be like turning on the light. How can we apply conversation analysis, usually concerned with the hidden aspects of plainly visible talk, to conversation in dark social spaces, across platforms and in private back channels? There is evidence that the norms of refusal, as highlighted by conversation analysis, are persistent across platforms, including in people's private digitally-mediated conversations. One of the ways in which we can identify these norms in action is by examining technology resistance. Relational communication via mobile device is pervasive (Hall and Baym). The concentration of digitally-mediated communication into smartphones means that conversational norms are constantly renegotiated, alongside expectations of relationship maintenance in voluntary social relationships like friendship (Hall and Baym). Mannell also explains that technology resistance can include lying by text message when explaining non-availability. These small, habitual, and often automatic lies are categorised as “butler lies” and are a polite way of achieving refusal in digitally mediated conversations that are analogous to how refusal is accomplished in face-to-face conversation. Refusals, rejections, and, by extension, unavailability appear to be accompanied by the palliative actions that help us achieve refusal in face-to-face conversation. Mannell identifies strategies such as “feeling ill” to explain non-availability without hurting others' feelings. Insights from conversation analysis suggest that on balance, it is likely that all parties involved in both the furnishing and acceptance of a butler lie understand that these are polite fabrications, much like the refusals in verbal conversation. Because of their invisibility, it is easy to assume that conversations in the dark social are chaotic and disorganised. However, there are tantalising hints that the reverse is true. Instead of arguing that individuals construct conversational norms on a case by case, platform by platform basis, I suggest that we now have a stock of common-sense social knowledge that we also apply to cross-platform mediated communication. In the spaces where gaps in this knowledge exist, Szabla and Blommaert argue that actors use existing norms of interactions and can navigate a range of interaction events even in online environments where we would expect to see a degree of context collapse and interactional disorganisation. Techniques of Detection How do we see in the dark? Some nascent research suggests a way forward that will help us understand the rhythms of cross-platform mediated conversation. Apps have been used to track participants' messaging and calling activities (Birnholtz, Davison, and Li). This research found a number of patterns that signal a user's attention or inattention, including response times and linguistic clues. Similarly, not-for-profit newsroom The Markup built a Facebook inspector called the citizen browser, a "standalone desktop application that was distributed to a panel of more than 1000 paid participants" (Mattu et al.). The application works by being connected to a participant's Facebook account and periodically capturing data from their Facebook feeds. The data is automatically deidentified but is still linked to the demographic information that participants provide about themselves, such as gender, race, location, and age. Applications like these point to how researchers might reliably collect interaction data from Facebook to glimpse into the hidden networks and interactions that drive conversation. User-focussed data collection methods also help us, as researchers, to sever our reliance on API access. API-reliant research is dependent on the largesse of social media companies for continued access and encourages research on the macro at the micro's expense. After all, social media and other digital platforms are partly constituted by the social acts of their users. Without speech acts that constitute mediated conversation, liking, sharing GIFs, and links, as well as the gaps and silences, digital platforms cease to exist. Digital platforms are not just archives of “big data”, but rather they are collections of speech and records of how our common-sense knowledge about how to communicate has stretched and expanded beyond face-to-face contexts. A Problem of Bots Ethnomethodological approaches have been critiqued as focussing too much on the small details of conversation, on nit-picking small details, and thus, as unable to comment on macro social issues of oppression and inequality (Kitzinger and Frith 311). However, understanding digitally-mediated conversation through the lens of talk-as-human-interaction may help us untangle our most pressing social problems across digital platforms. Extensive research examines platforms such as Twitter for “inauthentic” behaviour, primarily identifying which accounts are bots. Bots accounts are programmed Twitter accounts (for example) that automatically tweet information on political or contentious issues, while mimicking genuine engagement. Bots can reply to direct messages too; they converse with us as they are programmed to act as “humanly” as possible. Despite this, there are patterns of behaviour and engagement that distinguish programmed bot accounts, and a number of platforms are dedicated to their detection. However, bots are becoming increasingly sophisticated and better able to mimic “real” human engagement online. But there is as yet no systematic framework regarding what “real” digitally mediated conversation looks like. An ethnomethodological approach to understanding this would better equip platforms to understand inauthentic activity. As Yang and colleagues succinctly state, "a supervised machine learning tool is only as good as the data used for its training … even the most advanced [bot detection] algorithms will fail with outdated training datasets" (8). On the flipside, organisations are using chat bots to deliver cognitive behavioural therapy and assist people in moments of psychological distress. But the bots do not feel human; they reply instantly to any message sent. Some require responses in the form of emojis. The basis of therapy is talk. Understanding more accurately how naturally-occurring talk functions in online spaces could create more sensitive and genuinely therapeutic tools. Conclusion It is easy to forget that social media have largely mainstreamed over the last decade; in this decade, crucial social norms about how we converse online have developed. These norms allow us to navigate our conversations, with intimate friends and strangers alike across platforms, both in and out of public view, in ways that are often temporally non-sequential. Dark social spaces are a matter of intense marketing interest. Advertising firm Disruptive Advertising identified the very spaces that are the focus of this article as “dark social”: messaging apps, direct messaging, and native mobile apps facilitate user activity that is "not as easily controlled nor tracked". Dark social traffic continues to grow, yet our understanding of why, how, and for whom trails behind. To make sense of our social world, which is increasingly indistinguishable from online activity, we need to examine the spaces between and behind platforms, and how they co-mingle. Where are the spaces where the affordances of multiple platforms and technologies scrape against each other in uncomfortable ways? How do users achieve intelligible conversation not just because of affordances, but despite them? Focussing on micro-sociological encounters and conversations may also help us understand what could build a healthy online ecosystem. How are consensus and agreement achieved online? What are the persistent speech acts (or text acts) that signal when consensus is achieved? To begin where I started, to understand the scope and power of anti-vaccination sentiment, we need to understand how it is shared and discussed in dark social spaces, in messaging applications, and other backchannel spaces. Taking an ethnomethodological approach to these conversational interactions could also help us determine how misinformation is refused, accepted, and negotiated in mediated conversation. Focussing on “dark conversation” will help us more richly understand our social world and add much needed insight into some of our pressing social problems. References Burchell, Kenzie. 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