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ABSTRACT Ehrlichia chaffeensis is an obligately intracellular gram-negative bacterium and is the etiologic agent of human monocytic ehrlichiosis (HME). Although E. chaffeensis induces the generation of several cytokines and chemokines by leukocytes, E. chaffeensis lacks lipopolysaccharide and peptidoglycan. Bioinfomatic analysis of the E. chaffeensis genome, however, predicted genes encoding 15 lipoproteins and 3 posttranslational lipoprotein-processing enzymes. The present study showed that by use of multidimensional liquid chromatography followed by tandem mass spectrometry, all predicted lipoproteins as well as lipoprotein-processing enzymes were expressed by E. chaffeensis cultured in the human promyelocytic leukemia cell line HL-60. Consistent with this observation, a signal peptidase II inhibitor, globomycin, was found to inhibit E. chaffeensis infection and lipoprotein processing in HL-60 cell culture. To study in vivo E. chaffeensis lipoprotein expression and host immune responses to E. chaffeensis lipoproteins, 13 E. chaffeensis lipoprotein genes were cloned into a mammalian expression vector. When the DNA constructs were inoculated into naïve dogs, or when dogs were infected with E. chaffeensis, the animals developed delayed-type hypersensitivity reactions at cutaneous sites of the DNA construct deposition and serum antibodies to these lipoproteins. This is the first demonstration of lipoprotein expression and elicitation of immune responses by a member of the order Rickettsiales. Multiple lipoproteins expressed by E. chaffeensis in vitro and in vivo may play key roles in pathogenesis and immune responses in HME.
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Polyakov, L. M., D. V. Sumenkova, R. A. Knyazev, and L. E. Panin. "The analysis of interaction between lipoproteins and steroid hormones." Biomeditsinskaya Khimiya 57, no. 3 (2011): 308–13. http://dx.doi.org/10.18097/pbmc20115703308.
Using the methods of ultracentrifugation, gel-filtration and fluorescence quenching, we demonstrated, that plasma lipoproteins bind steroid hormones and can therefore play a role of their active transport form in an organism. High density lipoproteins have revealed the highest affinity to steroids for. It has been found, that protein component of lipoproteins takes part in the formation of lipoprotein-steroid complex. The apolipoprotein A-I, the main protein component of high density lipoproteins, is responsible for binding of steroid hormones. The calculated constants formation of the complexes of lipoproteins with steroid hormones testifies to specificity of linkage. The results obtained allow to considering real opportunity of transfer of steroid hormones into cell by a receptor-mediated endocytosis in structure of lipoproteins complexes.
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März, W., R. Siekmeier, H. Scharnagl, U. B. Seiffert, and W. Gross. "Fast lipoprotein chromatography: new method of analysis for plasma lipoproteins." Clinical Chemistry 39, no. 11 (November 1, 1993): 2276–81. http://dx.doi.org/10.1093/clinchem/39.11.2276.
Abstract Fast lipoprotein chromatography (FLPC) is a novel method for quantifying lipoproteins. Plasma proteins are separated by fast-flow gel filtration. Lipoproteins are detected by post-column derivatization with an enzymatic cholesterol reagent. FLPC resolves very-low-, low-, and high-density lipoproteins (VLDL, LDL, and HDL, respectively) and completely separates apolipoprotein Al- and apolipoprotein B-containing lipoproteins. CVs for VLDL-cholesterol, LDL-cholesterol, and HDL-cholesterol are 5.8%, 2.0%, and 1.9%, respectively. We compared FLPC with a combined ultracentrifugation and precipitation method and obtained correlation of r = 0.979, 0.978, and 0.933 for VLDL-cholesterol, LDL-cholesterol, and HDL-cholesterol, respectively. Triglyceride concentrations up to 9.00 g/L did not interfere with the quantification of lipoproteins by FLPC. We conclude that FLPC is a precise and reliable method for the analysis of plasma lipoproteins that complements conventional techniques.
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Remans, Kim, Ken Vercammen, Josselin Bodilis, and Pierre Cornelis. "Genome-wide analysis and literature-based survey of lipoproteins in Pseudomonas aeruginosa." Microbiology 156, no. 9 (September 1, 2010): 2597–607. http://dx.doi.org/10.1099/mic.0.040659-0.
Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen able to cause acute or chronic infections. Like all other Pseudomonas species, P. aeruginosa has a large genome, >6 Mb, encoding more than 5000 proteins. Many proteins are localized in membranes, among them lipoproteins, which can be found tethered to the inner or the outer membrane. Lipoproteins are translocated from the cytoplasm and their N-terminal signal peptide is cleaved by the signal peptidase II, which recognizes a specific sequence called the lipobox just before the first cysteine of the mature lipoprotein. A majority of lipoproteins are transported to the outer membrane via the LolCDEAB system, while those having an avoidance signal remain in the inner membrane. In Escherichia coli, the presence of an aspartate residue after the cysteine is sufficient to cause the lipoprotein to remain in the inner membrane, while in P. aeruginosa the situation is more complex and involves amino acids at position +3 and +4 after the cysteine. Previous studies indicated that there are 185 lipoproteins in P. aeruginosa, with a minority in the inner membrane. A reanalysis led to a reduction of this number to 175, while new retention signals could be predicted, increasing the percentage of inner-membrane lipoproteins to 20 %. About one-third (62 out of 175) of the lipoprotein genes are present in the 17 Pseudomonas genomes sequenced, meaning that these genes are part of the core genome of the genus. Lipoproteins can be classified into families, including those outer-membrane proteins having a structural role or involved in efflux of antibiotics. Comparison of various microarray data indicates that exposure to epithelial cells or some antibiotics, or conversion to mucoidy, has a major influence on the expression of lipoprotein genes in P. aeruginosa.
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Storf, Stefanie, Friedhelm Pfeiffer, Kieran Dilks, Zhong Qiang Chen, Saheed Imam, and Mechthild Pohlschröder. "Mutational and Bioinformatic Analysis of Haloarchaeal Lipobox-Containing Proteins." Archaea 2010 (2010): 1–11. http://dx.doi.org/10.1155/2010/410975.
A conserved lipid-modified cysteine found in a protein motif commonly referred to as a lipobox mediates the membrane anchoring of a subset of proteins transported across the bacterial cytoplasmic membrane via the Sec pathway. Sequenced haloarchaeal genomes encode many putative lipoproteins and recent studies have confirmed the importance of the conserved lipobox cysteine for signal peptide processing of three lipobox-containing proteins in the model archaeonHaloferax volcanii. We have extended thesein vivoanalyses to additionalHfx. volcaniisubstrates, supporting our previousin silicopredictions and confirming the diversity of predictedHfx. volcaniilipoproteins. Moreover, using extensive comparative secretome analyses, we identified genes encodining putative lipoproteins across a wide range of archaeal species. While ourin silicoanalyses, supported byin vivodata, indicate that most haloarchaeal lipoproteins are Tat substrates, these analyses also predict that many crenarchaeal species lack lipoproteins altogether and that other archaea, such as nonhalophilic euryarchaeal species, transport lipoproteins via the Sec pathway. To facilitate the identification of genes that encode potential haloarchaeal Tat-lipoproteins, we have developed TatLipo, a bioinformatic tool designed to detect lipoboxes in haloarchaeal Tat signal peptides. Our results provide a strong foundation for future studies aimed at identifying components of the archaeal lipoprotein biogenesis pathway.
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Mahmoodi, Bakhtawar, Ron Gansevoort, Friso Muntinghe, Robin Dullaart, Hanneke Kluin-Nelemans, Nic Veeger, Inge van Schouwenburg, and Karina Meijer. "Lipid levels do not influence the risk of venous thromboembolism." Thrombosis and Haemostasis 108, no. 11 (2012): 923–29. http://dx.doi.org/10.1160/th12-06-0426.
SummaryStudies on the association between lipid profile and venous thromboembolism (VTE) are inconsistent. This could be caused by classical lipoproteins being inferior to apolipoproteins as markers for VTE risk. Therefore, we examined whether apolipoproteins are more strongly related to VTE than lipoproteins. For this analysis we used the PREVEND prospective community based observational cohort study. Levels of apolipoprotein A1 (ApoA1), apolipoprotein B (ApoB), total cholesterol (TC), high-density lipoprotein (HDL), non-HDL, low-density lipoprotein (LDL), triglycerides (TG), lipoprotein(a), ApoB/ApoA1 and TC/HDL ratio were assessed. Subjects with VTE were identified using databases of the national registries of hospital discharge diagnoses, death certificates, and the regional anticoagulation clinic. Out of 7,627 subjects, 110 developed VTE during a median follow-up of 10.5 years. In both univariate and multivariable analyses no significant associations between apolipoproteins and overall VTE were observed. Of the classical lipoproteins, TC, non-HDL, LDL, TG, and TC/HDL ratio were significantly associated with overall VTE in univariate analysis. Significant associations were no longer present in multivariable analysis. TGL and LDL were significantly associated with unprovoked VTE in univariate analysis. After adjustment for age and sex this significance was lost. No significant associations between (apo-) lipoproteins and provoked VTE were found. We conclude that apolipoproteins are not better in predicting VTE risk than the classical lipoproteins. Our population-based cohort study does not show an association between both apolipoproteins and the classical lipoproteins and VTE risk.
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Trentalance, A., G. Bruscalupi, L. Conti Devirgiliis, S. Leoni, M. T. Mangiantini, L. Rossini, S. Spagnuolo, and S. K. Erickson. "Changes in lipoprotein binding and uptake by hepatocytes during rat liver regeneration." Bioscience Reports 9, no. 2 (April 1, 1989): 231–41. http://dx.doi.org/10.1007/bf01116000.
The binding and uptake of cholesterol enriched lipoproteins by isolated hepatocytes was decreased at 16 hours after partial hepatectomy, with a tendency to return to control values as the regeneration proceeds. The number of lipoprotein binding sites of total cellular membranes remained similar to control at 16 and 24 hours. The plasma lipoprotein pattern, determined by electrophoretic analysis, showed a lower per cent of very low density lipoproteins (VLDL) and a higher per cent of low density lipoproteins (LDL) at 16 and 24 hours post-partial hepatectomy. At these times, plasma lecithin: cholesterol acyltransferase (LCAT) activity was decreased. It is intriguing to suggest that the regenerating liver could regulated the blood lipoprotein pattern and the uptake of lipoproteins by modulating the surface expression of the receptors.
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Edelberg, J. M., M. Weissler, and S. V. Pizzo. "Kinetic analysis of the effects of glycosaminoglycans and lipoproteins on urokinase-mediated plasminogen activation." Biochemical Journal 276, no. 3 (June 15, 1991): 785–91. http://dx.doi.org/10.1042/bj2760785.
The glycosaminoglycans (GAGs) heparin, heparan sulphate and chondroitin 6-sulphate stimulate the rate of urokinase activation of human plasminogen. Kinetic analysis of plasminogen activation demonstrates that heparin, heparan sulphate and chondroitin 6-sulphate increased the catalytic rate (Kcat) by 5.3-, 3.5- and 2.5-fold respectively. These stimulatory GAGs had no effect on the affinity of urokinase for plasminogen, since the Km of the reaction is unaltered by the GAGs. The GAGs may enhance the rate of plasminogen activation through an interaction with the catalytic domain of the urokinase, with dissociation constants of approx. 30 nM. Additionally, the lipoproteins, lipoprotein (a) [Lp(a)] and low-density lipoprotein (LDL) inhibit heparin and heparan sulphate stimulation of plasmin formation. Lp(a) is a competitive inhibitor (Kic 20 nM) and LDL is a mixed inhibitor of heparin-enhanced urokinase-mediated plasminogen activation (Kic 24 nM and Kiu 60 nM). These inhibition constants correlate with physiological concentrations of these lipoproteins. These data suggest that these GAGs and lipoproteins may play an important role in vivo in regulating urokinase-mediated plasmin formation.
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Asmal, A. Cader. "Kinetic Analysis of Lipoproteins." JAMA: The Journal of the American Medical Association 253, no. 7 (February 15, 1985): 978. http://dx.doi.org/10.1001/jama.1985.03350310060015.
Asmal, A. C. "Kinetic analysis of lipoproteins." JAMA: The Journal of the American Medical Association 253, no. 7 (February 15, 1985): 978–79. http://dx.doi.org/10.1001/jama.253.7.978.
Dissertations / Theses on the topic "Lipoproteins Analysis":
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Barrett, P. Hugh R. "The kinetic analysis and computer modelling of lipoprotein metabolism in man." Title page, table of contents and abstract only, 1989. http://web4.library.adelaide.edu.au/theses/09PH/09phb274.pdf.
Leigh, Spencer A. "Functional analysis of the mycoplasma fermentans P29 adhesin." free to MU campus, to others for purchase, 2000. http://wwwlib.umi.com/cr/mo/fullcit?p9999300.
Chandra, Richa. "The analysis of triglyceride-rich lipoproteins in human serum for clinical studies." [College Station, Tex. : Texas A&M University, 2006. http://hdl.handle.net/1969.1/ETD-TAMU-1745.
Kung, Sin-yan. "Analysis of vitellogenin gene (MeVg2) from the sand shrimp (Metapenaeusensis): gene organization andexpression study." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B30497292.
Huang, Haibin. "Analysis of lipoproteins, outer membrane proteins, and genetic diversities of Ehrlichia and Anaplasma species." Columbus, Ohio : Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1155154668.
Ayyobi, Amir Fardad. "Analysis of lecithin : cholesterol acyltransferase (LCAT) protein structure and its influence on binding to plasma lipoproteins." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0014/NQ56499.pdf.
YAMADA, SHIN'YA, KATSUMI YAMANAKA, SHIN'YA ISHIHARA, HISATAKA SAKAKIBARA, TAKA-AKI KONDO, MASASHI FURUTA, and MASARU MIYAO. "The Relationship of High-Density Lipoprotein Cholesterol to Obesity, Drinking and Smoking Habits." Nagoya University School of Medicine, 1993. http://hdl.handle.net/2237/17534.
Conway, James Patrick. "Systems biology analysis of macrophage foam cells finding a novel function for Peroxiredoxin I /." Connect to text online, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=case1156961185.
Thesis (Ph. D.)--Case Western Reserve University, 2006. [School of Medicine] Department of Physiology and Biophysics. Includes bibliographical references. Available online via OhioLINK's ETD Center.
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Amigó, Grau Núria. "Lipoprotein analysis by 2d diffusion-ordered 1h-nmr spectroscopy." Doctoral thesis, Universitat Rovira i Virgili, 2017. http://hdl.handle.net/10803/665148.
The need for predictors of cardiovascular disease is increasing due to the pandemic levels that metabolic disorders are achieving. One of the main areas where new biomarkers can be looked for is in the lipids and lipoproteins field. NMR spectroscopy appears to be a suitable analytical tool to develop novel methodologies, which will aid in the assessment and management of cardiovascular events and cardio-metabolic disease, due to its sensitivity to the lipoprotein content and size, robustness and the possibility of automation.
Part of the research presented in this thesis is focused on the development and industrialization of the Liposcale test: an advanced lipoprotein test based on 2D diffusion ordered NMR spectroscopy (2D-DOSY NMR), born in a pure research framework, aiming to prepare it for the clinical use. Additionally, the scientific interest for the results derived from the lipoprotein characterization by 2D-DOSY NMR has been demonstrated over the course of the present thesis as reflected by the participation in several research studies from diverse areas of application, in which the test has given insights into the lipoprotein metabolism.
Alternatively, we have used 2D-DOSY NMR experiments for the study of the HDL isolated fraction for the evaluation and monitoring of diabetic dislipemia in combination with two other biophysical techniques -Atomic Force Microscopy (AFM) and fluorimetry, to explore lipoprotein properties beyond lipid content, such as the molecular lipoprotein structure and surface; favoring to understand their functionality in processes that deal with metabolic disorders.
Finally, the thesis explores how non-pharmacological nutritional interventions and diet modulates the lipoprotein profile; and how its characterization by using NMR can be used as an alternative to the traditional lipid characterization being sensitive to the lipoprotein distribution between subfractions and size, and therefore a better tool to evaluate different types of interventions, both pharmacological and nutritional.
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Douvris, Adrianna. "Functional Analysis of the TRIB1 Locus in Coronary Artery Disease." Thèse, Université d'Ottawa / University of Ottawa, 2011. http://hdl.handle.net/10393/20115.
The TRIB1 locus (8q24.13) is a novel locus associated with plasma TGs and CAD risk. Trib1 is a regulator of MAPK activity, and has been shown to regulate hepatic lipogenesis and VLDL production in mice. However, the functional relationship between common SNPs at the TRIB1 locus and plasma lipid traits is unknown; TRIB1 has not been identified as an eQTL. This cluster of SNPs falls within an intergenic region 25kb to 50kb downstream of the TRIB1 coding region. By phylogenetic footprinting analysis and DNA genotyping, we identified an evolutionarily conserved region (CNS1) within the risk locus that harbours two common SNPs in tight LD with GWAS risk SNPs and significantly associated with CAD. We investigated the regulatory function of CNS1 by luciferase reporter assays in HepG2 cells and demonstrate that this region has promoter activity. In addition, the rs2001844 risk allele significantly reduces luciferase activity, suggesting that altered expression of the EST-based gene may be associated with plasma TGs. We identified an EST within the risk locus directly downstream of CNS1. We performed 5'/3' RACE using HepG2 RNA, identified multiple variants of this EST-based gene, and confirmed its transcription start site within CNS1. We hypothesize that this EST is a long noncoding RNA due to low abundance, poor conservation, and absence of significant ORF. Over-expression of a short variant implicates its function in the regulation of target gene transcription, although the mechanism of action remains unknown. We conclude that the risk locus at 8q24.13 harbours a novel EST-based gene that may explain the relationship between GWAS SNPs at this locus and plasma lipid traits.
National Cholesterol Education Program (U.S.). Working Group on Lipoprotein Measurement. Recommendations on lipoprotein measurement: From the Working Group on Lipoprotein Measurement. [Bethesda, MD]: National Institutes of Health, National Heart, Lung and Blood Institute, 1995.
Workshop on Lipoprotein Heterogeneity (1986 Rockville, Md.). Proceedings of the Workshop on Lipoprotein Heterogeneity, Rockville, Maryland, September 29, 30, and October 1, 1986. Edited by Lippel Kenneth. [Bethesda, Md.]: U.S. Dept. of Health and Human Services, National Institutes of Health, 1987.
Walker, Janice. Analysis of the long term effect of physical activity level on high-density and low-density lipoproteins. Ottawa: National Library of Canada = Bibliothèque nationale du Canada, 1992.
International Atherosclerosis Conference (3rd 1983 Vienna, Austria). Detection and treatment of lipid and lipoprotein disorders of childhood: Proceedings of the Third International Atherosclerosis Conference, held in Vienna, Austria, April 4-9, 1983. Edited by Widhalm Kurt and Naito Herbert K. New York: Liss, 1985.
Weyer, Karl Aloys. Isolierung und Sequenzierung der Proteinuntereinheiten des photosynthetischen Reaktionszentrums von Rhodopseudomonas viridis: Entdeckung und Strukturaufklärung des Lipoprotein-Membranankers der Cytochrom-Untereinheit. Gauting bei München: Intemann, 1987.
Book chapters on the topic "Lipoproteins Analysis":
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de Lalla, Oliver F., and John W. Gofman. "Ultracentrifugal Analysis of Serum Lipoproteins." In Methods of Biochemical Analysis, 459–78. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470110171.ch16.
Fruchart, Jean-Charles, Monique Koffigan, Catherine Fievet, Claude Cachera, Ngoc Vu Dac, Jean-Claude Gesquière, and Pascal Puchois. "Molecular Analysis of Lipoproteins: Clinical Applications." In Advances in Experimental Medicine and Biology, 225–31. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4684-1268-0_33.
Yang, Chao-Yuh, Natalia V. Valentinova, Manlan Yang, Zi-Wei Gu, John R. Guyton, and Antonio M. Gotto. "Immunological Approach to Study the Structure of Oxidized Low Density Lipoproteins." In Methods in Protein Structure Analysis, 327–34. Boston, MA: Springer US, 1995. http://dx.doi.org/10.1007/978-1-4899-1031-8_28.
Burillo, Elena, Jesus Vazquez, and Inmaculada Jorge. "Quantitative Proteomics Analysis of High-Density Lipoproteins by Stable 18O-Isotope Labeling." In Methods in Molecular Biology, 139–56. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-405-0_11.
Thomas, C. E. "Nitrone spin traps as reagents for the study of oxidative modification of low density lipoproteins: Implications for atherosclerosis." In Analysis of Free Radicals in Biological Systems, 127–43. Basel: Birkhäuser Basel, 1995. http://dx.doi.org/10.1007/978-3-0348-9074-8_10.
Lehmann, Rainer. "Lipoprotein Analysis." In Clinical and Forensic Applications of Capillary Electrophoresis, 113–44. Totowa, NJ: Humana Press, 2001. http://dx.doi.org/10.1007/978-1-59259-120-6_7.
King, Sarah M., and Ronald M. Krauss. "Ion Mobility Lipoprotein Analysis." In Contemporary Cardiology, 537–44. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-56514-5_28.
Sasaki, Jun. "Molecular Analysis of Apolipoprotein A-I and E Mutants in Japan." In Lipoprotein Metabolism and Atherogenesis, 44–47. Tokyo: Springer Japan, 2000. http://dx.doi.org/10.1007/978-4-431-68424-4_7.
Miyake, Yasuko, Taku Yamamura, Keiko Oi, and Hideshi Hori. "Molecular Analysis on the LDL Receptors in Two Patients with Homozygous Familial Hypercholesterolemia." In Lipoprotein Metabolism and Atherogenesis, 59–61. Tokyo: Springer Japan, 2000. http://dx.doi.org/10.1007/978-4-431-68424-4_12.
Conference papers on the topic "Lipoproteins Analysis":
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Robbins, David L., John P. Nolan, James H. Jett, Richard A. Keller, and Larry A. Sklar. "Analysis of individual lipoproteins and liposomes." In BiOS '97, Part of Photonics West, edited by Gerald E. Cohn and Steven A. Soper. SPIE, 1997. http://dx.doi.org/10.1117/12.274353.
Damirchi, Behzad, Amir Rouhollahi, Salman Sohrabi, and Seyyed Mahdi Nemati Mehr. "Modeling and Stability Analysis of Truncated High Density Lipoprotein (HDL) System Using Martini Coarse Grain Technique." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-64808.
Lipoproteins are biochemical compounds containing both proteins and lipids. These particles carry chemicals like cholesterol and triglycerides that are not soluble in aqueous solutions. This paper presents modeling of lipoprotein system using coarse grain molecular dynamics technique and stability analysis of this system in a water solution like blood. A high density lipoprotein (HDL) that consists of two annular monomers is modeled. Also there are lipid bilayers located in center of the rings, so the whole HDL and lipid bilayers are called lipoprotein system. First, all atom model is provided and then coarse-grain model is obtained using MARTINI technique. Modeling of the system in all atom and coarse-grain is performed by VMD and simulation is executed by NAMD. System is simulated for 400ns with time step of 20fs in NPT ensemble. System temperature assumed similar to normal human body temperature. Finally the structure shape and stability of system were considered and results were analyzed.
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Aursnes, I., P. Smith, and H. Arnesen. "EPIDEMIOLOGICAL ASPECTS OF ANTITHROMBIN-III, SELENIUM AND LIPOPROTEIN COMPONENTS IN CORONARY DISEASE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643030.
The known risk factors for coronary disease can only “explain” a proportion of the incidence of the disease. Looking for supplementary risk factors we thus selected for detailed study both a group of patients with normal levels of risk factors (normo-tensives, non-smokers with normal serum cholesterol) and a group with high conventional risk factors, comparing both groups with an age and sex matched control group. Subgroups were formed by individuals aged below (young) and above (old) 60 years.Total- and HDL-cholesterol, apo-lipoproteins A-I and B and triglycerides showed co-variation with each others and with fatty acids in serum. With "factor analysis" seven "factors" were extracted and the factor scores for sub-groups were calculated. Two factors discriminated between young, high risk patients and controls. One was a positive risk factor and the other a negative one. The factors may be dependent on the existence of two unknown sub-groups of serum lipoproteins which were characterized by high concentrations of certain fatty acids.Coronary patients were found to have 9.1% higher antithrombin-III (AT-III) activity in their plasma than controls (p=0.037). Plasma selenium levels were slightly less in patients than in controls. There was a slight, but significant (r = 0.29 , p = 0.01 5) positive correlation between selenium and AT-III concentrations. Multivariate statistical anlysis indicated that selenium was significantly negatively correlated with disease.It is concluded that antithrombin-III tend to be high and that plasma selenium levels are relatively sub-normal in some coronary patients. It is also suggested that fatty acid analysis may be useful in the characterization of lipoproteins that are involved in the development of atherosclerosis.
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Prabhu, Anmiv S., Alejandro Moraga, Michael Cecchini, Rafael Mulero, Stephen Olsen, Young I. Cho, and Min Jun Kim. "Synthetic Nanoscale Architectures for Lipoprotein Separation." In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-66535.
Current low density lipoprotein (LDL) apheresis procedures are expensive and time consuming. We report here a novel technique to detect and separate nanoparticles using solid state nanopores. Our technique relies on the resistive pulse phenomenon used in coulter counters. We used a 150nm diameter nanopore to detect nanoparticles that closely resembled HDL and LDL in terms of their size and surface charge. Statistical analysis of the translocation data revealed that our setup preferentially allowed the particles resembling HDL to pass thorough while restricting the translocation of the particles that resembled LDL.
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Adelita, Sela Putri, Eti Poncorini Pamungkasari, and Bhisma Murti. "Meta Analysis: The Effect of High-Intensity Interval Training on Low Density Lipoprotein Level in Patients with Type 2 Diabetes Melitus." In The 7th International Conference on Public Health 2020. Masters Program in Public Health, Universitas Sebelas Maret, 2020. http://dx.doi.org/10.26911/the7thicph.05.42.
ABSTRACT Background: High intensity interval training (HIIT) is a protocol of short work intervals of vigorous to high intensity interspersed with active or passive (cessation of movement) recovery periods. HIIT has been employed since the mid-20th century to improve athletic exercise performance. Regular exercise reduces elevated low-density lipoprotein (LDL), atherosclerosis formation, and risk factors of cardiovascular disease (CVD). This study aimed to examine the effect of high-intensity interval training on low density lipoprotein level in patients with type 2 diabetes mellitus (DM). Subjects and Method: This was meta-analysis and systematic review. The study was conducted by search published article from year 2010 to 2020 in PubMed, Science Direct, Research Gate, and Google Scholar databases. The inclusion criteria were full text, using randomized controlled trial study design, high-intensity interval training intervention, and reporting mean and standard deviation. Study subjects were type 2 DM patients aged 25-65 years. The study outcome was LDL reduction. The articles were analyzed by PRISMA flow chart and Revman 5.3. Results: 7 studies from America, Europe, Australia, and Asia showed that high intensity interval training reduced LDL level in type 2 DM patients (Mean Difference= -0.06; 95% CI= 1.32 to -0.47; p<0.001) with I2= 92% (p <0.93). Conclusion: High-intensity interval training reduces LDL level in type 2 DM patients. Keywords: high-intensity interval training, low density lipoprotein Correspondence: Sela Putri Adelita. Masters Program in Public Health, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta 57126, Central Java. Email: Selaadelita558@gmail.com. Mobile: 085357117517. DOI: https://doi.org/10.26911/the7thicph.05.42
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Zinman, B., C. Mathieu, S. Kaspers, HJ Woerle, and D. Fitchett. "Empagliflozin reduces mortality in analyses adjusted for control of blood pressure, low density lipoprotein cholesterol and HbA1c over time." In Diabetes Kongress 2018 – 53. Jahrestagung der DDG. Georg Thieme Verlag KG, 2018. http://dx.doi.org/10.1055/s-0038-1641901.
Roan, Esra, Alex Bada, and Randy Buddington. "Mechanical Characterization of Preterm Neonate Pig Liver as a Function of High-Density Lipoprotein (HDL)." In ASME 2010 International Mechanical Engineering Congress and Exposition. ASMEDC, 2010. http://dx.doi.org/10.1115/imece2010-39363.
Elastography, a non-invasive imaging modality, utilizes mechanical properties of tissue as markers for disease diagnosis or staging. In the case of liver, there have been a number of studies focusing on the relationship between elastic mechanical properties and underlying disease, i.e. fibrosis and cirrhosis. In summary, these studies indicate the feasibility of elastographic tools in detecting liver diseases such as fibrosis and steatosis. There have not been any studies looking at the mechanical properties of the preterm neonate liver to date, which is important, because preterm neonates are at a greater risk for developing liver complications due to their aggressive dietary needs that are met with total parenteral nutrition (TPN). They use of elastography may be less from the use of elastographic tools since the concerns over noise levels in measurements resulting from abdominal wall thickness may be less influential. Therefore, it is necessary to establish basic preterm neonate liver mechanical properties. In this study, we measured the nonlinear (hyperelastic) mechanical properties of livers from preterm pigs that were fed common neaonatal diets, i.e. colostrum, total parenteral nutrition (TPN). 16 neonate pigs survived the feeding regime. Mechanical evaluation of 15 of these neonatal pigs was achieved with the use of uniaxial compression experiments at 0.01 s−1 strain rate. The livers averaging a weight of 34.7±7.0 (SD), were stored in phosphate buffered saline solution at 4°C until experimentation, which occurred within 30 minutes of the animal sacrifice. A minimum of three specimens from each liver was required for the computation of averaged mechanical properties. In addition to mechanical testing samples, blood serum was also obtained from these animals and common chemical parameters for liver health were measured (bilirubin, ALT, AST, HDL, LDL, etc.) Exponential form of the hyperelastic strain energy function, W = b1exp[b2(L2 + 2/L-3)], where bi are the material parameters and L is the stretch ratio, was utilized to describe the hyperelastic mechanical behavior of the preterm neonate pig livers. With the use of E = 6b1b2, a small-strain regime estimate of the elastic modulus of the neonate liver tissue was also computed. The mean b1 and b2 parameters are determined to be 97.00±44.15(SD) Pa and 1.90±0.28(SD) (n = 71). The mean elastic modulus exhibited an linear dependence on the HDL values obtained from chemical analysis of the blood serum. Moreover, although relatively weak, the ratio of the HDL over LDL also correlated with the elastic modulus. To our knowledge, this is the only study to date that has focused on the mechanical properties of preterm neonatal pigs and its correlation with liver lipid profile in neonates. Future work will focus on correlating this information with histology and then devising multi-scale material characterization approaches that link underlying neonatal liver structure to its overall mechanical properties.
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Hassall, D. C., R. F. G. Booth, A. C. Honey, and J. F. Martin. "EXTRAVASCULAR INJURY CAUSES FOAM CELL FORMATION, ACCUMULATION OF CHOLESTEROL (C) and CHOLESTEROL ESTER (CE) IN THE CAROTID ARTERY." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643414.
In atherosclerotic arterial tissue, cholesterol is delivered to smooth muscle cells by low-density lipoprotein (LDL) and to macrophages via modified LDL. The reasons for accumulation of excess lipid are unknown although increased uptake of C and CE occurs in regions of arterial de-endothelialisation. We now report that the positioning of a silastic collar containing saline around the outside of arteries induces accumulation of C and CE within those tissues. 9 rabbits were separated into 3 groups, each group was fed a normal laboratory chow, 2 groups were supplemented with lg/day cholesterol; these rabbits were used for C and CE determinations. Three parallel groups of rabbits were set up for histological analysis. At day O, under anaesthesia, a silastic collar was* placed around the left carotid artery. The collar was filled with saline and carefully sealed without causing constriction of the vessel. The vessels were replaced and the animals allowed to recover. After 14 days the carotids from animals for C and CE determination were rapidly removed and divided up into a region from the middle of the collar, a region proximal to the collar and a distal region. The tissues were freeze clamped in liquid nitrogen and lipids extracted with C and CE determined for each region. The carotids for histological analysis were perfuse-fixed in situ and similarly subdivided.We conclude 1) Animals fed high cholesterol accumulate more C than CE within arterial tissue, furthermore this accumulation is greater than in animals fed a normal diet 2) In both normal diet and high cholesterol fed animals an enhanced accumulation of C + CE occurs within arteries with a collar 3) CE appears to be preferentially accumulated within the collar region.
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Scheefers, H., A. Kobus, and R. Geyer. "CARBOHYDRATE COMPOSITION AND LECTIN BINDING AFFINITIES OF HUMAN PLACENTAL TISSUE FACTOR." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643737.
Tissue factor (TF) is a widely distibuted membrane glycoprotein and the most potent trigger of bloodcoagulation. It serves as an essential cofactor for the activation of Factor IX and X by Factor Vll/VIIa.TF is a lipoprotein composed of a phospholipid portion and a glycosylated apoprotein (apo-TF). The procoagulant activity of bovine brain TF is inhibited bythe lectin Con A indicating that the carbohydrates of TF might play a functional role in its interactionwith Factor Vll/VIIa.In the present study apo-TF was purified from human placenta by repeated SDS-PAGE to a purity of 95%. The carbohydrates of apo-TF wereanalyzed by capillary gas- liquid-chromatography andmass-fragmentography. This analysis revealed that apo-TF contains about 16% (w/w) carbohydrate consistingof 50.4 mole% N-acetylglucosamine, 22.2 mole% mannose, 21.0 mole% galactose, 3.2 mole% fucose and 3.2 mole% N-acetylgalactosamine. Further information on the structure of the carbohydrate moieties of the apoTF was achieved by determining the binding affinities of the apo-TF to ten different lectins. For this purpose a semiquantitative spot lectino sorbent assaywas developed. This assay is based on the detection of peroxidase-labeled lectins after being bound to the carbohydrate moieties of apo-TF adsorbed onto a nitrocellulose membrane. Human placental apo-TF showed the strongest affinity to wheat germ agglutinin which specifically binds to N-acetylglucosamine and sialic acid residues.In contrast to bovine brain apo-TF, human placental apo-TF only weakly interacted with Con A, which is known to recognize mannosyl residues in mannose-rich, hybrid- and biantennary glycans,but not in tri- or tetraantennary oligosaccharides of the complex type. From the carbohydrate constituent analysis and from the lectin binding studies it can be concluded that human placental apo-TF carriesabout four N-linked higher branched oligosaccharide chains.
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Weigensberg, B. I., M. Alavi, S. Moore, J. O. Lough, H. Riml, and M. H. Lee. "PROTEOGLYCAN IN AORTIC WHITE MURAL THROMBUS AND IN RESULTING THROMBOATHEROSCLEROTIC LESIONS IN RABBITS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643408.
Thromboatherosclerotic lesions in normolipidemic rabbits may contain variable amounts of lipid but the cause of this variable lipid content is not understood. Proteoglycan (PG) has been implicated in the trapping of lipoproteins in atherosclerosis. The object of this investigation was to study the quantity and quality of PG and glycosaminoglycan (GAG ) in early white mural thrombus induced with an indwelling catheter in the aorta of the rabbit as it evolves into thrombo atherosclerosis and to determine if the Alcian Blue stainable PG correlates with lipid accumulation. Lesions were compared with the normal aortic wall and a gut Alcian Blue standard embedded in the same block and cut and stained with Alcian Blue under the same conditions and analysed with a computerized color system that measures unstained lipid and Alcian Blue in a delineated lesion. The normal rabbit aorta had a mean ALCIAN BLUE COLOR VALUE “ ABCV ”of 10 equivalent to 14 ug GAG per ( mg dlt) mg delipidated tissue. Early mural thrombus in normolipidemic rabbits had virtually no Alcian Blue stainable proteoglycan and sin ABCV of 1 to 2 . After 12 weeks the resulting thrombo atherosclerotic lesions had a mean ABCV of 28±12 ( equivalent to 39±17 ug GAG per mg dlt. The ABCV in areas with large numbers of macrophages laden with lipid droplets and platelet debris ranged from 5 to 15. It is concluded that proteoglycans play a less significant role in the accumulation of lipid and platelet debris by the macrophages and this lipid and platelet debris may regress readily; but probably plays a more important role in the accumulation of interstitial lipid adjacent to smooth muscle cells. The interstitial lipid and PG deposits adjacent to numerous smooth muscle cells may be more resistant to regression. These two types of lipid deposits which are both variable determines the total lipid concentration in these lesions.
Reports on the topic "Lipoproteins Analysis":
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Cheng, Wenke, Julia Boettner, Tina Fischer-Schaepman, Sarah Werner, Angela Kricke, Holger Thiele, and Petra Buettner. High-density lipoprotein Cholesterol Efflux Capacity and the Risk of Cardiovascular Diseases: A Systematic Review and Meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, July 2021. http://dx.doi.org/10.37766/inplasy2021.7.0006.
Wang, Zeng-mian. Association between serum lipoprotein levels and cognitive impairment in acute cerebral infarction: a protocol for systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review Protocols, April 2020. http://dx.doi.org/10.37766/inplasy2020.4.0018.
Wang, Zeng-mian. Association between serum lipoprotein levels and neurological function in patients with acute ischemic stroke: a protocol of systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, April 2020. http://dx.doi.org/10.37766/inplasy2020.4.0043.
