Relevant bibliographies by topics / Lipid membranes Biotechnology

Academic literature on the topic 'Lipid membranes Biotechnology'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Lipid membranes Biotechnology"

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Jackman, Joshua, Wolfgang Knoll, and Nam-Joon Cho. "Biotechnology Applications of Tethered Lipid Bilayer Membranes." Materials 5, no. 12 (December 7, 2012): 2637–57. http://dx.doi.org/10.3390/ma5122637.

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Efimova, S. S., T. E. Tertychnaya, S. N. Lavrenov, and O. S. Ostroumova. "The Mechanisms of Action of Triindolylmethane Derivatives on Lipid Membranes." Acta Naturae 11, no. 3 (September 15, 2019): 38–45. http://dx.doi.org/10.32607/20758251-2019-11-3-38-45.

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The effects of new synthetic antibacterial agents - tris(1-pentyl-1H-indol-3-yl)methylium chloride (LCTA-1975) and (1-(4-(dimethylamino)-2,5-dioxo-2,5-dihydro-1H-pyrrol-3-yl)-1H-indol-3-yl)bis(1-propyl- 1H-indol-3-yl)methylium chloride (LCTA-2701 - on model lipid membranes were studied. The ability of the tested agents to form ion-conductive transmembrane pores, influence the electrical stability of lipid bilayers and the phase transition of membrane lipids, and cause the deformation and fusion of lipid vesicles was investigated. It was established that both compounds exert a strong detergent effect on model membranes. The results of differential scanning microcalorimetry and measuring of the threshold transmembrane voltage that caused membrane breakdown before and after adsorption of LCTA-1975 and LCTA-2701 indicated that both agents cause disordering of membrane lipids. Synergism of the uncoupling action of antibiotics and the alkaloid capsaicin on model lipid membranes was shown. The threshold concentration of the antibiotic that caused an increase in the ion permeability of the lipid bilayer depended on the membrane lipid composition. It was lower by an order of magnitude in the case of negatively charged lipid bilayers than for the uncharged membranes. This can be explained by the positive charge of the tested agents. At the same time, LCTA-2701 was characterized by greater efficiency than LCTA-1975. In addition to its detergent action, LCTA-2701 can induce ion-permeable transmembrane pores: step-like current fluctuations corresponding to the opening and closing of individual ion channels were observed. The difference in the mechanisms of action might be related to the structural features of the antibiotic molecules: in the LCTA-1975 molecule, all three substituents at the nitrogen atoms of the indole rings are identical and represent n-alkyl (pentyl) groups, while LCTA-2701 contains a maleimide group, along with two alkyl substituents (n-propyl). The obtained results might be relevant to our understanding of the mechanism of action of new antibacterial agents, explaining the difference in the selectivity of action of the tested agents on the target microorganisms and their toxicity to human cells. Model lipid membranes should be used in further studies of the trends in the modification and improvement of the structures of new antibacterial agents.
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Schuster, Bernhard, and Uwe B. Sleytr. "S-layer-supported lipid membranes." Reviews in Molecular Biotechnology 74, no. 3 (September 2000): 233–54. http://dx.doi.org/10.1016/s1389-0352(00)00014-3.

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Beard, Jason, George S. Attard, and Matthew J. Cheetham. "Integrative feedback and robustness in a lipid biosynthetic network." Journal of The Royal Society Interface 5, no. 22 (October 16, 2007): 533–43. http://dx.doi.org/10.1098/rsif.2007.1155.

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The homeostatic control of membrane lipid composition appears to be of central importance for cell functioning and survival. However, while lipid biosynthetic reaction networks have been mapped in detail, the underlying control architecture which underpins these networks remains elusive. A key problem in determining the control architectures of lipid biosynthetic pathways, and the mechanisms through which control is achieved, is that the compositional complexity of lipid membranes makes it difficult to determine which membrane parameter is under homeostatic control. Recently, we reported that membrane stored elastic energy provides a physical feedback signal which modulates the activity in vitro of CTP:phosphocholine cytidylyltransferase (CCT), an extrinsic membrane enzyme which catalyses a key step in the synthesis of phosphatidylcholine lipids in the Kennedy pathway (Kennedy 1953 J. Am. Chem. Soc . 75 , 249–250). We postulate that stored elastic energy may be the main property of membranes that is under homeostatic control. Here we report the results of simulations based on this postulate, which reveal a possible control architecture for lipid biosynthesis networks in vivo .
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Schuster, Bernhard, and Uwe B. Sleytr. "Biomimetic interfaces based on S-layer proteins, lipid membranes and functional biomolecules." Journal of The Royal Society Interface 11, no. 96 (July 6, 2014): 20140232. http://dx.doi.org/10.1098/rsif.2014.0232.

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Designing and utilization of biomimetic membrane systems generated by bottom-up processes is a rapidly growing scientific and engineering field. Elucidation of the supramolecular construction principle of archaeal cell envelopes composed of S-layer stabilized lipid membranes led to new strategies for generating highly stable functional lipid membranes at meso- and macroscopic scale. In this review, we provide a state-of-the-art survey of how S-layer proteins, lipids and polymers may be used as basic building blocks for the assembly of S-layer-supported lipid membranes. These biomimetic membrane systems are distinguished by a nanopatterned fluidity, enhanced stability and longevity and, thus, provide a dedicated reconstitution matrix for membrane-active peptides and transmembrane proteins. Exciting areas in the (lab-on-a-) biochip technology are combining composite S-layer membrane systems involving specific membrane functions with the silicon world. Thus, it might become possible to create artificial noses or tongues, where many receptor proteins have to be exposed and read out simultaneously. Moreover, S-layer-coated liposomes and emulsomes copying virus envelopes constitute promising nanoformulations for the production of novel targeting, delivery, encapsulation and imaging systems.
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Dymond, Marcus K., Charlotte V. Hague, Anthony D. Postle, and George S. Attard. "An in vivo ratio control mechanism for phospholipid homeostasis: evidence from lipidomic studies." Journal of The Royal Society Interface 10, no. 80 (March 6, 2013): 20120854. http://dx.doi.org/10.1098/rsif.2012.0854.

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While it is widely accepted that the lipid composition of eukaryotic membranes is under homeostatic control, the mechanisms through which cells sense lipid composition are still the subject of debate. It has been postulated that membrane curvature elastic energy is the membrane property that is regulated by cells, and that lipid composition is maintained by a ratio control function derived from the concentrations of type II and type 0 lipids, weighted appropriately. We assess this proposal by seeking a signature of ratio control in quantified lipid composition data obtained by electrospray ionization mass spectrometry from over 40 independent asynchronous cell populations. Our approach revealed the existence of a universal ‘pivot’ lipid, which marks the boundary between type 0 lipids and type II lipids, and which is invariant between different cell types or cells grown under different conditions. The presence of such a pivot species is a distinctive signature of the operation in vivo , in human cell lines, of a control function that is consistent with the hypothesis that membrane elastic energy is homeostatically controlled.
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Avis, Tyler J., Mélanie Michaud, and Russell J. Tweddell. "Role of Lipid Composition and Lipid Peroxidation in the Sensitivity of Fungal Plant Pathogens to Aluminum Chloride and Sodium Metabisulfite." Applied and Environmental Microbiology 73, no. 9 (March 2, 2007): 2820–24. http://dx.doi.org/10.1128/aem.02849-06.

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ABSTRACT Aluminum chloride and sodium metabisulfite have shown high efficacy at low doses in controlling postharvest pathogens on potato tubers. Direct effects of these two salts included the loss of cell membrane integrity in exposed pathogens. In this work, four fungal potato pathogens were studied in order to elucidate the role of membrane lipids and lipid peroxidation in the relative sensitivity of microorganisms exposed to these salts. Inhibition of mycelial growth in these fungi varied considerably and revealed sensitivity groups within the tested fungi. Analysis of fatty acids in these fungi demonstrated that sensitivity was related to high intrinsic fatty acid unsaturation. When exposed to the antifungal salts, sensitive fungi demonstrated a loss of fatty acid unsaturation, which was accompanied by an elevation in malondialdehyde content (a biochemical marker of lipid peroxidation). Our data suggest that aluminum chloride and sodium metabisulfite could induce lipid peroxidation in sensitive fungi, which may promote the ensuing loss of integrity in the plasma membrane. This direct effect on fungal membranes may contribute, at least in part, to the observed antimicrobial effects of these two salts.
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Huffer, Sarah, Melinda E. Clark, Jonathan C. Ning, Harvey W. Blanch, and Douglas S. Clark. "Role of Alcohols in Growth, Lipid Composition, and Membrane Fluidity of Yeasts, Bacteria, and Archaea." Applied and Environmental Microbiology 77, no. 18 (July 22, 2011): 6400–6408. http://dx.doi.org/10.1128/aem.00694-11.

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ABSTRACTIncreased membrane fluidity, which causes cofactor leakage and loss of membrane potential, has long been documented as a cause for decreased cell growth during exposure to ethanol, butanol, and other alcohols. Reinforcement of the membrane with more complex lipid components is thus thought to be beneficial for the generation of more tolerant organisms. In this study, organisms with more complex membranes, namely, archaea, did not maintain high growth rates upon exposure to alcohols, indicating that more complex lipids do not necessarily fortify the membrane against the fluidizing effects of alcohols. In the presence of alcohols, shifts in lipid composition to more saturated and unbranched lipids were observed in most of the organisms tested, including archaea, yeasts, and bacteria. However, these shifts did not always result in a decrease in membrane fluidity or in greater tolerance of the organism to alcohol exposure. In general, organisms tolerating the highest concentrations of alcohols maintained membrane fluidity after alcohol exposure, whereas organisms that increased membrane rigidity were less tolerant. Altered lipid composition was a common response to alcohol exposure, with the most tolerant organisms maintaining a modestly fluid membrane. Our results demonstrate that increased membrane fluidity is not the sole cause of growth inhibition and that alcohols may also denature proteins within the membrane and cytosol, adversely affecting metabolism and decreasing cell growth.
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Moore, Eli K., Ellen C. Hopmans, W. Irene C. Rijpstra, Laura Villanueva, Svetlana N. Dedysh, Irina S. Kulichevskaya, Hans Wienk, Frans Schoutsen, and Jaap S. Sinninghe Damsté. "Novel Mono-, Di-, and Trimethylornithine Membrane Lipids in Northern Wetland Planctomycetes." Applied and Environmental Microbiology 79, no. 22 (August 30, 2013): 6874–84. http://dx.doi.org/10.1128/aem.02169-13.

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ABSTRACTNorthern peatlands represent a significant global carbon store and commonly originate fromSphagnummoss-dominated wetlands. These ombrotrophic ecosystems are rain fed, resulting in nutrient-poor, acidic conditions. Members of the bacterial phylumPlanctomycetesare highly abundant and appear to play an important role in the decomposition ofSphagnum-derived litter in these ecosystems. High-performance liquid chromatography coupled to high-resolution accurate-mass mass spectrometry (HPLC-HRAM/MS) analysis of lipid extracts of four isolated planctomycetes from wetlands of European north Russia revealed novel ornithine membrane lipids (OLs) that are mono-, di-, and trimethylated at the ε-nitrogen position of the ornithine head group. Nuclear magnetic resonance (NMR) analysis of the isolated trimethylornithine lipid confirmed the structural identification. Similar fatty acid distributions between mono-, di-, and trimethylornithine lipids suggest that the three lipid classes are biosynthetically linked, as in the sequential methylation of the terminal nitrogen in phosphatidylethanolamine to produce phosphatidylcholine. The mono-, di-, and trimethylornithine lipids described here represent the first report of methylation of the ornithine head groups in biological membranes. Various bacteria are known to produce OLs under phosphorus limitation or fatty-acid-hydroxylated OLs under thermal or acid stress. The sequential methylation of OLs, leading to a charged choline-like moiety in the trimethylornithine lipid head group, may be an adaptation to provide membrane stability under acidic conditions without the use of scarce phosphate in nutrient-poor ombrotrophic wetlands.
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Efimova, S. S., and O. S. Ostroumova. "Dipole Modifiers Regulate Lipid Lateral Heterogeneity in Model Membranes." Acta Naturae 9, no. 2 (June 15, 2017): 67–74. http://dx.doi.org/10.32607/20758251-2017-9-2-67-74.

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In this study we report on experimental observations of giant unilamellar liposomes composed of ternary mixtures of cholesterol (Chol), phospholipids with relatively low Tmelt (DOPC, POPC, or DPoPC) and high Tmelt (sphingomyelin (SM), or tetramyristoyl cardiolipin (TMCL)) and their phase behaviors in the presence and absence of dipole modifiers. It was shown that the ratios of liposomes exhibiting noticeable phase separation decrease in the series POPC, DOPC, DPoPC regardless of any high-Tmelt lipid. Substitution of SM for TMCL led to increased lipid phase segregation. Taking into account the fact that the first and second cases corresponded to a reduction in the thickness of the lipid domains enriched in low- and high-Tmelt lipids, respectively, our findings indicate that the phase behavior depends on thickness mismatch between the ordered and disordered domains. The dipole modifiers, flavonoids and styrylpyridinium dyes, reduced the phase segregation of membranes composed of SM, Chol, and POPC (or DOPC). The other ternary lipid mixtures tested were not affected by the addition of dipole modifiers. It is suggested that dipole modifiers address the hydrophobic mismatch through fluidization of the ordered and disordered domains. The ability of a modifier to partition into the membrane and fluidize the domains was dictated by the hydrophobicity of modifier molecules, their geometric shape, and the packing density of domain-forming lipids. Phloretin, RH 421, and RH 237 proved the most potent among all the modifiers examined.
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Dissertations / Theses on the topic "Lipid membranes Biotechnology"

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Danial, John Shokri Hanna. "Imaging lipid phase separation on droplet interface bilayers." Thesis, University of Oxford, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.711943.

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Villar, Gabriel. "Aqueous droplet networks for functional tissue-like materials." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:602f9161-368c-48c0-9619-7974f743f2f2.

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An aqueous droplet in a solution of lipids in oil acquires a lipid monolayer coat, and two such droplets adhere to form a bilayer at their interface. Networks of droplets have been constructed in this way that function as light sensors, batteries and electrical circuits by using membrane proteins incorporated into the bilayers. However, the droplets have been confined to a bulk oil phase, which precludes direct communication with physiological environments. Further, the networks typically have been assembled manually, which limits their scale and complexity. This thesis addresses these limitations, and thereby enables prospective medical and technological applications for droplet networks. In the first part of the work, defined droplet networks are encapsulated within mm-scale drops of oil in water to form structures called multisomes. The encapsulated droplets adhere to one another and to the surface of the oil drop to form interface bilayers that allow them to communicate with each other and with the surrounding aqueous environment through membrane pores. The contents of the droplets can be released by changing the pH or temperature of the surrounding solution. Multisomes have potential applications in synthetic biology and medicine. In the second part of the work, a three-dimensional printing technique is developed that allows the construction of complex networks of tens of thousands of heterologous droplets ~50 µm in diameter. The droplets form a self-supporting material in bulk oil or water analogous to biological tissue. The mechanical properties of the material are calculated to be similar to those of soft tissues. Membrane proteins can be printed in specific droplets, for example to establish a conductive pathway through an otherwise insulating network. Further, the networks can be programmed by osmolarity gradients to fold into designed shapes. Printed droplet networks can serve as platforms for soft devices, and might be interfaced with living tissues for medical applications.
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Thompson, James Russell. "Imaging the assembly of the Staphylococcal pore-forming toxin alpha-Hemolysin." Thesis, University of Oxford, 2009. http://ora.ox.ac.uk/objects/uuid:e320004a-6118-4dac-af2a-eca6e90be7ac.

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Alpha-hemolysin is a pore-forming toxin secreted by pathogenic Staphylococcus aureus. Its spontaneous oligomerization and assembly into a trans-bilayer beta-barrel pore is a model for the assembly of many other pore-forming toxins. It is studied here in vitro as a means to probe general membrane protein oligomerization and lipid bilayer insertion. This thesis details the results of experiments to develop and implement a novel in vitro lipid bilayer system, Droplet-on-Hydrogel Bilayers (DHBs) for the single-molecule imaging of alpha-hemolysin assembly. Chapter 2 describes the development of DHBs and their electrical characterization. Experiments show the detection of membrane channels in SDS-PAGE gels post-electrophoresis and DHBs use as a platform for nanopore stochastic sensing. Chapter 3 describes the engineering and characterization of fluorescently-labelled monomeric alpha-hemolysin for use in protein assembly imaging experiments described in Chapter 6. Chapter 4 describes the characterization of DHB lipid fluidity and suitability for single-molecule studies of membrane protein diffusion. In addition, a novel single-particle tracking algorithm is described. Chapter 5 describes experiments demonstrating simultaneous electrical and fluorescence measurements of alpha-hemolysin pores embedded within DHBs. The first multiple-pore stochastic sensing in a single-lipid bilayer is also described. Chapter 6 describes experiments studying the assembly of alpha-hemolysin monomers in DHBs. Results show that alpha-hemolysin assembles rapidly into its oligomeric state, with no detection of long-lived intermediate states.
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Li, Pin. "Effects of carbon nanotubes on airway epithelial cells and model lipid bilayers : proteomic and biophysical studies." Thesis, 2014. http://hdl.handle.net/1805/5968.

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Indiana University-Purdue University Indianapolis (IUPUI)
Carbon nanomaterials are widely produced and used in industry, medicine and scientific research. To examine the impact of exposure to nanoparticles on human health, the human airway epithelial cell line, Calu-3, was used to evaluate changes in the cellular proteome that could account for alterations in cellular function of airway epithelia after 24 h exposure to 10 μg/mL and 100 ng/mL of two common carbon nanoparticles, singleand multi-wall carbon nanotubes (SWCNT, MWCNT). After exposure to the nanoparticles, label-free quantitative mass spectrometry (LFQMS) was used to study differential protein expression. Ingenuity Pathway Analysis (IPA) was used to conduct a bioinformatics analysis of proteins identified by LFQMS. Interestingly, after exposure to a high concentration (10 μg/mL; 0.4 μg/cm2) of MWCNT or SWCNT, only 8 and 13 proteins, respectively, exhibited changes in abundance. In contrast, the abundance of hundreds of proteins was altered in response to a low concentration (100 ng/mL; 4 ng/cm2) of either CNT. Of the 281 and 282 proteins that were significantly altered in response to MWCNT or SWCNT, respectively, 231 proteins were the same. Bioinformatic analyses found that the proteins common to both kinds of nanotubes are associated with the cellular functions of cell death and survival, cell-to-cell signaling and interaction, cellular assembly and organization, cellular growth and proliferation, infectious disease, molecular transport and protein synthesis. The decrease in expression of the majority proteins suggests a general stress response to protect cells. The STRING database was used to analyze the various functional protein networks. Interestingly, some proteins like cadherin 1 (CDH1), signal transducer and activator of transcription 1 (STAT1), junction plakoglobin (JUP), and apoptosis-associated speck-like protein containing a CARD (PYCARD), appear in several functional categories and tend to be in the center of the networks. This central positioning suggests they may play important roles in multiple cellular functions and activities that are altered in response to carbon nanotube exposure. To examine the effect of nanotubes on the plasma membrane, we investigated the interaction of short purified MWCNT with model lipid membranes using a planar bilayer workstation. Bilayer lipid membranes were synthesized using neutral 1, 2-diphytanoylsn-glycero-3-phosphocholine (DPhPC) in 1 M KCl. The ion channel model protein, Gramicidin A (gA), was incorporated into the bilayers and used to measure the effect of MWCNT on ion transport. The opening and closing of ion channels, amplitude of current, and open probability and lifetime of ion channels were measured and analyzed by Clampfit. The presence of an intermediate concentration of MWCNT (2 μg/ml) could be related to a statistically significant decrease of the open probability and lifetime of gA channels. The proteomic studies revealed changes in response to CNT exposure. An analysis of the changes using multiple databases revealed alterations in pathways, which were consistent with the physiological changes that were observed in cultured cells exposed to very low concentrations of CNT. The physiological changes included the break down of the barrier function and the inhibition of the mucocillary clearance, both of which could increase the risk of CNT’s toxicity to human health. The biophysical studies indicate MWCNTs have an effect on single channel kinetics of Gramicidin A model cation channel. These changes are consistent with the inhibitory effect of nanoparticles on hormone stimulated transepithelial ion flux, but additional experiments will be necessary to substantiate this correlation.
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Books on the topic "Lipid membranes Biotechnology"

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Ottova-Leitmannova, Angelica, and H. Ti Tien. Advances in planar lipid bilayers and liposomes. Edited by Iglic Ales. Amsterdam: Elsevier/Academic Press, 2005.

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Paul, Gaber Bruce, Schnur Joel M, Chapman Dennis 1927-, and Workshop on Biotechnological Applications of Membranes Studies (1987 : San Sebastian, Spain), eds. Biotechnological applications of lipid microstructures. New York: Plenum Press, 1988.

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Hanke, W. Planar lipid bilayers: Methods and applications. London: Academic Press, 1993.

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Paul, Gaber Bruce, Schnur Joel M, and Chapman Dennis 1927-, eds. Biotechnological applications of lipid microstructures. Plenum, 1988.

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Liu, A. Leitmannova. Advances in Planar Lipid Bilayers and Liposomes, Volume 6 (Advances in Planar Lipid Bilayers and Liposomes) (Advances in Planar Lipid Bilayers and Liposomes). Academic Press, 2007.

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Book chapters on the topic "Lipid membranes Biotechnology"

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Lindholm-Sethson, Britta. "Supported Lipid Membranes for Reconstitution of Membrane Proteins." In Physics and Chemistry Basis of Biotechnology, 131–65. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/0-306-46891-3_5.

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Grainger, D. W., K. M. Maloney, X. Huang, M. Ahlers, A. Reichert, H. Ringsdorf, C. Salesse, J. N. Herron, V. Hlady, and K. Lim. "Binding, Interaction, and Organization of Proteins with Lipid Model Membranes." In Progress in Membrane Biotechnology, 64–82. Basel: Birkhäuser Basel, 1991. http://dx.doi.org/10.1007/978-3-0348-7454-0_6.

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Nieva, José-Luis, and Alicia Alonso. "On the Mechanism of Phospholipase C-Induced Fusion of Pure Lipid Membranes." In Progress in Membrane Biotechnology, 177–94. Basel: Birkhäuser Basel, 1991. http://dx.doi.org/10.1007/978-3-0348-7454-0_12.

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Chrost, B., J. Falk, B. Kernebeck, H. Mölleken, and K. Krupinska. "Tocopherol Biosynthesis in Senescing Chloroplasts - A Mechanism to Protect Envelope Membranes against Oxidative Stress and a Prerequisite for Lipid Remobilization ?" In The Chloroplast: From Molecular Biology to Biotechnology, 171–76. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4788-0_27.

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Keough, K. M. W., J. Pérez-Gil, G. Simatos, J. Tucker, K. Nag, C. Boland, J. Stewart, et al. "Hydrophobic Pulmonary Surfactant Proteins in Model Lipid Systems." In Progress in Membrane Biotechnology, 241–52. Basel: Birkhäuser Basel, 1991. http://dx.doi.org/10.1007/978-3-0348-7454-0_17.

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Gaber, Bruce Paul, David C. Turner, Krishnan Namboodiri, William R. Light, and Albert Hybl. "Tools for Molecular Graphics Depictions of Lipid Structures." In Progress in Membrane Biotechnology, 40–51. Basel: Birkhäuser Basel, 1991. http://dx.doi.org/10.1007/978-3-0348-7454-0_4.

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Quinn, P. J., and L. J. Lis. "Dynamic X-Ray Diffraction Studies of Phase Transitions in Lipid-Water Systems." In Progress in Membrane Biotechnology, 12–29. Basel: Birkhäuser Basel, 1991. http://dx.doi.org/10.1007/978-3-0348-7454-0_2.

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Wirtz, K. W. A., T. W. J. Gadella, J. Verbist, P. J. Somerharju, and A. J. W. G. Visser. "Fluorescent Analogues of Phosphoinositides in Studies on Lipid-Protein Interactions and Membrane Dynamics." In Progress in Membrane Biotechnology, 52–63. Basel: Birkhäuser Basel, 1991. http://dx.doi.org/10.1007/978-3-0348-7454-0_5.

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Han, Xiaojun, Guodong Qi, Xingtao Xu, and Lei Wang. "Lipid Bilayer Membrane Arrays: Fabrication and Applications." In Advances in Biochemical Engineering/Biotechnology, 121–52. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/10_2012_135.

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Krull, U. J., R. S. Brown, R. N. Koilpillai, R. Nespolo, and E. T. Vandenberg. "Receptor Modulated State-Switching of Lipid Membrane Biosensors." In Biotechnology Research and Applications, 165–74. Dordrecht: Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-009-1371-4_16.

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Conference papers on the topic "Lipid membranes Biotechnology"

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Lee, HeaYeon, and JuKyung Lee. "Advanced Biomimetic Nanodevice Using Nanotechnology Addressable Lipid Rafts Nanoarrays Toward Advanced Nanomaterials." In ASME 2013 2nd Global Congress on NanoEngineering for Medicine and Biology. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/nemb2013-93286.

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In recent years, a new paradigm of nanobiomedical devices combining miniaturization and integration has been exploited in areas such as combinational chemistry, biotechnology, engineering, proteomics and clinical diagnostics. One of the critical issues in the development of nanobiomedical system is how to differentiate signal-to-noise ratio per very small amount of signal. Biocompatible integrated nanopattern requires the fabrication of appropriately designed nanomatrix for high sensitivity homogenous assays, which are capable of ultimately mimic the physiological environment. We reported the nanomatrix geometry of a well-oriented nanowell array derived from nanofabrication technology which can easily be employed for digital detection with a high S/N ratio, miniaturization, integrated assays and single molecule analysis. In this present, we describe a nano(submicro) array of tethered lipid bilayer raft membranes comprising a biosensing platform.
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"Analysis of the distribution of parameter of membrane lipid phase state." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-144.

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Huang, Yong, and Boris Rubinsky. "A Microfabricated Chip for the Study of Cell Electroporation." In ASME 2000 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2000. http://dx.doi.org/10.1115/imece2000-2233.

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Abstract It has been observed that when certain electrical potentials are applied across a cell they can induce the formation of pores in the cell membrane and consequently increase the permeability of the cell to macromolecules. This phenomenon is known as electroporation. Since the first report on gene transfer by electroporation1, it has become a standard method for introduction of macromolecules into cells2 3 4. Currently, electroporation is normally done in batches of cells between electrodes and there is little control over the permeabilization of individual cells. Therefore, it is very difficult to study the fundamental biophysics of cell membrane electro-permeabilization and to design optimal electroporation protocols for individual cells2 3 . Although the biophysics of electroporation are still not fully understood, indirect evidence shows that micro aqueous pores with diameters of tens to hundreds of angstroms are created in the cell membrane due to the electrical field induced structural rearrangement of the lipid bilayer5. It occurred to us that if electroporation induces pores in the cell membrane, then in a state of electroporation, a measurable current should flow through the individual cell. From this idea, we have developed a new micro-electroporation technology that employs a “bionic” chip to study and control the electroporation process in individual cells. The micro-electroporation chip, shown schematically in Figure 1, is designed and fabricated using standard silicon microfabrication technology. Each chip is a three-layer device that consists of two translucent poly silicon electrodes and a silicon nitride membrane, which all together form two fluid chambers. The two chambers are interconnected only through a micro hole through the dielectric silicon nitride membrane. In a typical process, the two chambers are filled with conductive solutions and one chamber contains biological cells. Individual cells can be captured in the micro hole and thus incorporated into the electrical circuit between the two electrodes of the chip. When the cell is in its normal state no current flows through the insulating lipid bilayer and consequently between the electrodes. However, when the electrical potential across the electrodes is sufficient to induce electroporation, a measurable current will flow through the pores of the cell membrane and between the electrodes. Measuring the currents through the bionic chip in real time will reveal the information of the state of electropermeabilization in cell membrane. The breakdown potential of irreversible electroporation, the most critical parameter in electroporation process, can be detected by analyzing current signals as well. Figure 2 illustrates a typical electrical signature in an irreversible electroporation process. Once the target cell is electroporated by the application of sufficient electroporation electrical potentials, macromolecules that are normally impermeant to cell membrane can be uploaded into the cell. Figure 3 shows how a cell entrapped in a hole is loaded during electroporation with a fluorescent die. With the ability to manipulate individual cells and detect the electrical potentials that induce electroporation in each cell, the chip can be used to study the fundamental biophysics of membrane electropermeabilization on the single cell level and in biotechnology, for controlled introduction of macromolecules, such as DNA fragments, into individual cells. We anticipate that this new technology will change the way in which electroporation is done and will provide key understanding of the biophysical processes that lead to cell electroporation. This paper will discuss the design, fabrication of the micro-electroporation chip, the experiment system as well as experiments carried out to precisely detect the parameters of electroporation of individual biological cells.
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4

Huang, Yong, and Boris Rubinsky. "A Microfabricated Chip for the Study of Cell Electroporation." In ASME 2000 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2000. http://dx.doi.org/10.1115/imece2000-2496.

Full text
Abstract:
Abstract It has been observed that when certain electrical potentials are applied across a cell they can induce the formation of pores in the cell membrane and consequently increase the permeability of the cell to macromolecules. This phenomenon is known as electroporation. Since the first report on gene transfer by electroporation1, it has become a standard method for introduction of macromolecules into cells2 3 4. Currently, electroporation is normally done in batches of cells between electrodes and there is little control over the permeabilization of individual cells. Therefore, it is very difficult to study the fundamental biophysics of cell membrane electro-permeabilization, which is not yet understood, and to design optimal and reversible electroporation protocols for individual cells2 3. Although the biophysics of electroporation are still not fully understood, indirect evidence shows that micro aqueous pores with diameters of tens to hundreds of angstroms are created in cell membrane due to the electrical field induced structural rearrangement of the lipid bilayer5. It occurred to us that if electroporation induces pores in the cell membrane than, in a state of electroporation, a measurable current should flow through the individual cell. From this idea, we have developed a new micro-electroporation technology that employs a “bionic” chip to study and control the electroporation process in individual cells. The micro-electroporation chips are designed and fabricated using standard silicon microfabrication technology. Figure 1 shows the schematic of the chip in cross section. Each chip is a three-layer device that consists of two translucent poly silicon electrodes and a silicon nitride membrane, which all together form two fluid chambers. The two chambers are interconnected only through a micro hole on the dielectric silicon nitride membrane. In a typical process, the two chambers are filled with conductive solutions and one chamber contains biological cells. Individual cells can be captured in the micro hole and thus incorporated in the electrical circuit between the two electrodes of the chip. When the cell is in its normal state no current flows through the insulating lipid bilayer and consequently between the electrodes. However, when the electrical potential across the electrodes is sufficient to induce electroporation, a measurable current will flow through the pores of the cell membrane and between the electrodes. Measuring currents through the bionic chip as a function of electrical potential will determine the potential that induces the electroporation. The chip behaves somewhat similarly to an electrical diode, with no current at potentials that do not induce electroporation and currents at potentials that induce electroporation. With the ability to manipulate individual cells and detect the electrical potentials that induce electroporation in each cell, the chip can be used to study the fundamental biophysics of membrane electro-permeabilization on single cell level and in biotechnology, for controlled introduction of macromolecules, such as gene constructs, into individual cells. We anticipate that this new technology will change the way in which electroporation is done and will provide key understanding of the biophysical processes that lead to cell electroporation. In this paper, first the design, fabrication process and modeling of the microelectroporation chip are described in details. Subsequently, experiment methods and results are presented and discussed, demonstrating the feasibility of altering cell membrane permeability and facilitating intercellular mass transfer in a more controlled way on single cell level. Finally, the potential applications of the micro-electroporation chips and future research directions are discussed. Figure 2 demonstrates how cell membrane electroporation can be investigated through monitoring and analyzing chip current-voltage signatures.
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