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Dissertations / Theses on the topic 'Lignocellulosic inhibitor'

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Published: 9 March 2023

Last updated: 31 July 2025

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1

Gasperoni, Alessia. "Removal of inhibitors from birch pretreatment liquor by nanofiltration: Mechanisms of separation and influence of operational variables and mode." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2018. http://amslaurea.unibo.it/15147/.

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Lignocellulosic biomass has attracted considerable attention as an alternative feedstock for the production of fuels, energy and chemicals, due to its renewability, abundance and reduced cost. Pretreatment and hydrolysation of lignocellulose releases sugars that are subsequently converted by fermentation. However, by-products such as aliphatic acids and furans could be generated during the upstream processes, which could inhibit enzymes and fermenting microorganisms. In addition, fermentation of low-concentrated sugars would lead to low products concentration and, consequently, to higher recov

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2

BERTAGNOLI, STEFANO. "Improving robustness and metabolic profile of saccharomyces cerevisiae for industrial bioprocesses." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2012. http://hdl.handle.net/10281/28926.

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The fossil energy resources decrease and climate changes, caused by carbon dioxide (CO2) emissions, have led most industrialized countries to undertake policies aimed at the development and use of renewable energy sources. Among the renewable energies, vegetal biomasses play a key role because widely available and potentially able to cover up to 200% of the global energy demand. Vegetal biomasses can be used mainly as raw materials for the production of chemicals, biofuels and energy, in the increasingly important green economy concept based on biorefineries creation. Although the vegetal biom

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3

Leung, Ka Kay. "Analysis of yeast resistance to lignocellulosic-derived inhibitors." Thesis, University of Nottingham, 2015. http://eprints.nottingham.ac.uk/32589/.

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The rapid depletion of fossil fuel reserves and concurrent increase in global temperatures has resulted in global demand for the production of alternative environmentally friendly fuels. First-generation biofuels that utilise cash crops for the extraction of fermentable sugars currently exist, but are highly controversial due to socioeconomic and environmental reasons such as diverting food production or deforestation. Therefore, second-generation biofuels that utilise lignocellulosic waste materials are a more attractive prospect. In Europe, lignocellulosic biomass wastes such as wheat straw,

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4

Frazão, Cláudio José Remédios. "Challenges of ethanol production from lignocellulosic biomass." Master's thesis, Universidade de Aveiro, 2014. http://hdl.handle.net/10773/13657.

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Mestrado em Biotecnologia - Biotecnologia Industrial e Ambiental<br>The present work aimed to tackle two of the major challenges in bioethanol production from lignocellulosic feedstocks: (i) high tolerance of microorganisms to lignocellulosic inhibitors, and (ii) microbial contamination avoidance. Lignocellulosic inhibitors are an important fraction of spent sulphite liquor (SSL), a by-product of the pulp and paper industries. Hardwood SSL (HSSL) is rich in pentose sugars, mainly xylose, which can be converted to ethanol by the yeast Scheffersomyces stipitis. In this work, a population of S.

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5

Du, Bowen Chambliss C. Kevin. "Effect of varying feedstock-pretreatment chemistry combinations on the production of potentially inhibitory degradation products in biomass hydrolysates." Waco, Tex. : Baylor University, 2009. http://hdl.handle.net/2104/5319.

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6

Boukari, Imen. "Définition des critères d'efficacité d'une hémicellulase pour l'hydrolyse de substrats lignocellulosiques complexes et insolubles." Reims, 2010. http://ebureau.univ-reims.fr/slide/files/quotas/SCD/theses/sciences/2010REIMS011.pdf.

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Le développement de technologies enzymatiques constitue un enjeu majeur pour le fractionnement maîtrisé et la valorisation des ressources lignocellulosiques (biocarburants, biopolymères, synthons…). L’efficacité de ces biocatalyseurs est cependant limitée par de multiples facteurs liés à la fois à leurs caractéristiques structurales et fonctionnelles, mais également à la nature complexe de la biomasse lignocellulosique (riche en parois secondaires lignifiées). Dans le but d’identifier les paramètres clés pour une conversion efficace des hémicelluloses, constituants majeurs des lignocelluloses,

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7

Cavka, Adnan. "Biorefining of lignocellulose : Detoxification of inhibitory hydrolysates and potential utilization of residual streams for production of enzymes." Doctoral thesis, Umeå universitet, Kemiska institutionen, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-82486.

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Lignocellulosic biomass is a renewable resource that can be utilized for the production of biofuels, chemicals, and bio-based materials. Biochemical conversion of lignocellulose to advanced biofuels, such as cellulosic ethanol, is generally performed through microbial fermentation of sugars generated by thermochemical pretreatment of the biomass followed by an enzymatic hydrolysis of the cellulose. The aims of the research presented in this thesis were to address problems associated with pretreatment by-products that inhibit microbial and enzymatic biocatalysts, and to investigate the potentia

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8

Zautsen, Remigius Reinerus Maria 1977. "Fermentação alcoólica e extração líquido-líquido simultânea de etanol e de inibidores provenientes de caldo hidrolítico de biomassa lignocelulósica." [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256499.

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Orientador: Francisco Maugeri Filho<br>Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos<br>Made available in DSpace on 2018-08-19T06:57:12Z (GMT). No. of bitstreams: 1 Zautsen_RemigiusReinerusMaria_D.pdf: 4385252 bytes, checksum: 3906f1725b3c49ed542429ca357c57ea (MD5) Previous issue date: 2011<br>Resumo: Na fermentação de produtos como etanol, utilizando biomassa lignocelulósica como matéria-prima, existem dois fatores principais que limitam a produtividade e eficiência do processo: inibição pelo produto e inibição por substâncias no caldo hidrolític

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9

Siqueira, Marcos Rechi. "Efeitos dos produtos de hidrólise de materiais lignocelulósicos sobre a produção de H2 por fermentação." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/59/59138/tde-13042015-114341/.

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O hidrogênio é uma fonte de energia limpa, pois sua combustão gera apenas água. Porém, ainda há a necessidade de se encontrar soluções tecnologicamente eficientes, econômicas e seguras para sua geração e uso. A produção do H2 por vias biológicas, conhecido como biohidrogênio, vem ganhando grande destaque nos últimos anos, pois possibilita o uso de materiais renováveis como matéria-prima. Materiais lignocelulósicos são potenciais substratos para a produção de H2 por fermentação, no entanto se faz necessário dispor de métodos de hidrólise que disponibilizem os componentes destes materiais para a

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10

Westman, Johan. "Ethanol production from lignocellulose using high local cell density yeast cultures. Investigations of flocculating and encapsulated Saccharomyces cerevisiae." Doctoral thesis, Högskolan i Borås, Institutionen Ingenjörshögskolan, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-3685.

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Efforts are made to change from 1st to 2nd generation bioethanol production, using lignocellulosics as raw materials rather than using raw materials that alternatively can be used as food sources. An issue with lignocellulosics is that a harsh pretreatment step is required in the process of converting them into fermentable sugars. In this step, inhibitory compounds such as furan aldehydes and carboxylic acids are formed, leading to suboptimal fermentation rates. Another issue is that lignocellulosics may contain a large portion of pentoses, which cannot be fermented simultaneously with glucose

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11

Jourdier, Etienne. "Modélisation et optimisation de la production de cellulases par Trichoderma reesei pour les bioraffineries lignocellulosiques." Thesis, Clermont-Ferrand 2, 2012. http://www.theses.fr/2012CLF22264.

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Dans le contexte énergétique et climatique mondial, le coût élevé des enzymes Cellulolytiques (cellulases) freine le développement des bioraffineries lignocellulosiques, pour produire des biocarburants et composés chimiques à partir d'une matière première végétale renouvelable. L'objectif de ce travail est de caractériser et de modéliser le métabolisme du micro-organisme Trichoderma reesei, afin d'optimiser le protocole industriel de production de cellulases. Cette étude a été réalisée sur des milieux modèles représentatifs de ceux attendus à l'échelle industrielle. Tout d'abord, la stoechiomé

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12

Axelsson, Josefin. "Separate Hydrolysis and Fermentation of Pretreated Spruce." Thesis, Linköpings universitet, Institutionen för fysik, kemi och biologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-69487.

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Bioethanol from lignocellulose is expected to be the most likely fuel alternative in the near future. SEKAB E-Technology in Örnsköldsvik, Sweden develops the technology of the 2nd generation ethanol production; to produce ethanol from lignocellulosic raw material. The objective of this master’s thesis was to achieve a better knowledge of the potential and limitations of separate hydrolysis and fermentation (SHF) as a process concept for the 2nd generation ethanol production. The effects of enzyme concentration, temperature and pH on the glucose concentration in the enzymatic hydrolysis were in

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13

Zhang, Yan. "Detoxification of Lignocellulose-derived Microbial Inhibitory Compounds by Clostridium beijerinckii NCIMB 8052 during Acetone-Butanol-Ethanol Fermentation." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1366384921.

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14

Basso, Thalita Peixoto. "Improvement of Saccharomyces cerevisiae by hybridization for increased tolerance towards inhibitors from second-generation ethanol substrate." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-29042015-132341/.

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Global climate change and volatility of petroleum price have driven the necessity to reduce fossil fuel utilization and replace it by renewable energy. Bioethanol production in the United States and Brazil from cornstarch and sugarcane, respectively, is already established. However, the bioethanol industry appears unsustainable in view of the potential stress that its production places on food commodities. In contrast, second-generation biofuels produced from cheap and abundant lignocellulosic biomass, has been viewed as one plausible solution to this \"food versus fuel\" problem. Sugarcane ba

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15

Gomes, Márcia Andréa. "Destoxificação de hidrolisados lignocelulósico visando à obtenção de etanol 2G." Universidade Federal de Alagoas, 2015. http://www.repositorio.ufal.br/handle/riufal/1203.

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The sugarcane bagasse has a high content of lignocellulosic material, which enables the study for the production of second-generation ethanol, requiring the application of a pretreatment that promotes the rupture of the fiber, to make accessible sugars for fermentation. There are several pretreatments aimed at the break and in the search for the most productive one, it is applied severe conditions of temperature and pressure. This promotes the formation of undesirable products in the bioethanol production process, requiring detoxification step for removal of inhibitors. In this study, we used

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16

Miranda, Elisângela de Souza. "Seleção de linhagens de Saccharomyces cerevisiae tolerantes aos inibidores presentes no hidrolisado de bagaço de cana-de-açúcar." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-29042016-175443/.

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A busca por soluções sustentáveis, levando a um processo energético mais eficiente induziu a novas tecnologias e esforços estão sendo realizados para viabilizar o etanol de segunda geração, com o aproveitamento da biomassa celulolítica como substrato para a fermentação alcoólica. Contudo, durante a hidrólise do bagaço, muitos compostos tóxicos à levedura são formados como o furfural, hidroximetilfurfural, ácido acético, e compostos fenólicos com efeitos depressivos sobre a fermentação. A adição de melaço no hidrolisado de bagaço poderia permitir uma fermentação com maior teor alcoólico, contri

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17

Silvello, Cristiane. "Obtenção de leveduras tolerantes aos inibidores do hidrolisado de bagaço de cana-de-açúcar mediante hibridação." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-26092016-133126/.

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O desenvolvimento de alternativas aos combustíveis fósseis como fonte de energia é uma prioridade global. A biomassa celulósica representa uma alternativa para satisfazer a procura de bicombustíveis renováveis. O bagaço de cana-de-açúcar é um abundante subproduto proveniente da produção atual de etanol no Brasil. Tal subproduto pode ser hidrolisado a fim de se obter açúcares fermentáveis para a produção do etanol de segunda-geração. Porém, no processo de pré-tratamento são gerados diversos inibidores como ácido acético, furfural e hidroximetilfurfural que causam efeitos adversos para a levedur

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18

Barbosa, Mariana de Almeida. "Estudos de inibição de β-glicosidases bacterianas por fenóis solúveis". Botucatu, 2019. http://hdl.handle.net/11449/181944.

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Orientador: Mario de Oliveira Neto<br>Resumo: A biomassa lignocelulósica pode ser usada para a produção de energia ou de novos bioprodutos potenciais substitutos de químicos convencionais. Porém a conversão dos polissacarídeos estruturais presentes na parede celular vegetal das células que compõe a biomassa não é simples. Isto se deve principalmente pela presença da lignina, que juntamente com a hemicelulose, formam uma estrutura coesa de microfibrilas que entrelaçam a celulose. Compostos que inibem as enzimas celulolíticas, incluindo fenólicos solúveis (derivados da lignina), açúcares solúvei

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19

Budde, Jörn. "Improving digestibility of cattle waste by thermobarical treatment." Doctoral thesis, Humboldt-Universität zu Berlin, Lebenswissenschaftliche Fakultät, 2015. http://dx.doi.org/10.18452/17191.

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Im Laborversuch konnte der positive Einfluss einer thermobarischen Vorbehandlung auf die Hydrolysier- und Vergärbarkeit von Rinderfestmist und Rindergülle nachgewiesen werden. Die Laborergebnisse wurden innerhalb eines theoretischen Modells in den Praxismaßstab übertragen, um den Einfluss auf Treibhausgasemissionen, Energiebilanz und Ökonomie zu bewerten. Die Vorbehandlungstemperaturen im Labor lagen zwischen 140 und 220°C in Schritten von 20 K und einer Vorbehandlungszeit von jeweils 5 Minuten. Die höchste Methanmehr¬ausbeute von 58 % konnte bei einer Temperatur von 180°C ermittelt werden. D

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20

Bin, Hussin Mohd Hazwan. "Extraction, modification and characterization of lignin from oil palm fronds as corrosion inhibitors for mild steel in acidic solution." Thesis, Université de Lorraine, 2014. http://www.theses.fr/2014LORR0135/document.

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La biomasse lignocellulosique en Malaisie peut être considérée comme l'une des sources d'énergie renouvelable prometteuse. Elle est principalement composée de cellulose, d'hémicellulose et de lignine et est adaptée pour des applications dans les domaines de l'énergie et de la chimie en raison de sa disponibilité suffisante, de son faible coût et de son caractère renouvelable. La production de biomasse lignocellulosique en Malaisie est considérée comme élevée et est issue en grande partie de l'industrie de l'huile de palme (environ 60 millions de tonnes de déchets d'huile de palme sont générés

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21

Cunha, Joana Filipa Torres Pinheiro. "Engineering yeast tolerance to inhibitory lignocellulosic biomass." Master's thesis, 2013. http://hdl.handle.net/1822/28630.

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Dissertação de mestrado em Bioengenharia<br>The progressive depletion of fossil fuels reserves in the last years led to the necessity for biotechnological manufacturing based on lignocellulosic feedstocks. Lignocellulosic biomass, such as straw, is an abundant low-cost source for production of biofuels, such as bioethanol, that does not compete for food needs. However, lignocellulose-to-ethanol process involves pre-treatment of biomass to obtain readily fermentable sugars, which leads to the accumulation of inhibitory by-products (e.g. furan derivatives, phenolic compounds, organic acids). Sig

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22

Richardson, Terri. "Strain improvement of Scheffersomyces stipitis for the bioconversion of lignocellulosic biomass into ethanol." Thesis, 2013. http://hdl.handle.net/10214/6624.

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Pretreatment of recalcitrant lignocellulosic biomass to release sugars for bioconversion into ethanol produces fermentation inhibitors. Increasing yeast inhibitor tolerance should reduce production time and cost. UV mutagenesis followed by genome shuffling using cross mating was performed on Scheffersomyces stipitis strain GS301, a genome shuffled strain with increased tolerance to spent sulphite liquor (SSL). The main fermentation inhibitors in SSL are acetic acid, hydroxymethylfurfural (HMF), and various phenolics. UV mutagenesis resulted in acetic acid tolerant mutants, but they were pheno

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23

"Inhibitory effects of acids found in crude glycerol and lignocellulosic biomass on clostridium pasteurianum for butanol production." Tulane University, 2020.

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archives@tulane.edu<br>As fossil fuel resources decrease while pollution and greenhouse gas emissions from gasoline increase, the need for an alternative transportation fuel is pressing (Cassia et al. 2018). Current substitutes include ethanol-mixed gasolines, which have lower efficiency than gasoline and still rely on fossil fuels. Butanol, another possible gasoline substitution, is promising. It is a much more efficient fuel than ethanol and can be directly substituted for gasoline (Seggiani et al. 2012). Current techniques to mass-produce butanol depend on fermentation of sugars for butanol

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24

TSAI, JIA-YIN, and 蔡佳吟. "Effect of lignocellulosic hydrolysis inhibitors on biotransforming 5-hydroxy-methylfurfural into 2,5-furan-dicarboxylic acid using immobilized cells of Burkholderia cepacia." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/n54ss3.

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碩士<br>國立雲林科技大學<br>環境與安全衛生工程系<br>105<br>Lignocellulose is the most abundant biomass on Earth, and its cellulosic polysaccharide is suitable used as raw material for bioenergy production. To release polysaccharide from lignocellulose and further hydrolyze polysaccharide into simple sugar, pretreatment is necessary and beneficial for subsequent fermentation process. Thermal acid hydrolysis is extensively applied and econamic pretreatment method to deal with lignocellulosic biomasses, but inhibitors are inevitably formed during this process. 5-Hydroxymethylfurfural (5-HMF) is the main inhibitor co

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Soudham, Venkata Prabhakar. "Biochemical conversion of biomass to biofuels : pretreatment–detoxification–hydrolysis–fermentation." Doctoral thesis, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-102722.

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The use of lignocellulosic materials to replace fossil resources for the industrial production of fuels, chemicals, and materials is increasing. The carbohydrate composition of lignocellulose (i.e. cellulose and hemicellulose) is an abundant source of sugars. However, due to the feedstock recalcitrance, rigid and compact structure of plant cell walls, access to polysaccharides is hindered and release of fermentable sugars has become a bottle-neck. Thus, to overcome the recalcitrant barriers, thermochemical pretreatment with an acid catalyst is usually employed for the physical or chemical disr

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